                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  HCV NS3/4A Replicon Shuttle Vectors

<130>  26299 WO

<150>  US 61/238426
<151>  2009-08-31

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector AsiSI sense primer

<400>  1
cggggacgtg gttttccttt gaaaaacgcg atcgcaccat ggcgcctatt acg              53


<210>  2
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector AsiSI anti-senser primer

<400>  2
cgtaataggc gccatggtgc gatcgcgttt ttcaaaggaa aaccacgtcc ccg              53


<210>  3
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector FseI sense primer

<400>  3
gatgagatgg aagggccggc ctcacacctc ccttacatcg                             40


<210>  4
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector FseI anti-senser primer

<400>  4
cgatgtaagg gaggtgtgag gccggccctt ccatctcatc                             40


<210>  5
<211>  10076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSC_1b_NS3/4A/lacZ_AsiSI_FseI

<400>  5
cctgcaggta atacgactca ctatagccag cccccgattg ggggcgacac tccaccatag       60

atcactcccc tgtgaggaac tactgtcttc acgcagaaag cgtctagcca tggcgttagt      120

atgagtgtcg tgcagcctcc aggacccccc ctcccgggag agccatagtg gtctgcggaa      180

ccggtgagta caccggaatt gccaggacga ccgggtcctt tcttggatca acccgctcaa      240

tgcctggaga tttgggcgtg cccccgcgag actgctagcc gagtagtgtt gggtcgcgaa      300

aggccttgtg gtactgcctg atagggtgct tgcgagtgcc ccgggaggtc tcgtagaccg      360

tgcaccgttt aaacccccgt gctgctggaa gtcgatttcg cttagggtaa ccgtggacct      420

cgaaaacaga cgcacaaaac caagttcaat agaagggggt acaaaccagt accaccacga      480

acaagcactt ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg      540

gatccgttat ccgcttatgt acttcgagaa gcccagtacc acctcggaat cttcgatgcg      600

ttgcgctcag cactcaaccc cagagtgtag cttaggctga tgagtctgga catccctcac      660

cggtgacggt ggtccaggct gcgttggcgg cctacctatg gctaacgcca tgggacgcta      720

gttgtgaaca aggtgtgaag agcctattga gctacataag aatcctccgg cccctgaatg      780

cggctaatcc caacctcgga gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg      840

caagtccgtg gcggaaccga ctactttggg tgtccgtgtt tccttttatt ttattgtggc      900

tgcttatggt gacaatcaca gattgttatc ataaagcgaa ttggattggc catccggtga      960

aagtgagact cattatctat ctgtttgctg gatccgctcc attgagtgtg tttactctaa     1020

gtacaatttc aacagttatt tcaatcagac aattgtatca taatggcggg cccagaagac     1080

gccaaaaaca taaaggaagg cccggcgcca ttctatcctc ttgaggatgg aaccgctgga     1140

gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca     1200

gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg     1260

gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa     1320

aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg     1380

cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct     1440

accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta     1500

ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg     1560

atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca     1620

gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg     1680

ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga     1740

gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc     1800

catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc     1860

ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa     1920

agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa     1980

tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc     2040

ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact     2100

gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt     2160

aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc     2220

gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac     2280

aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata     2340

gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa     2400

tacaaaggtt atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac     2460

atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc     2520

gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc     2580

agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg     2640

aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag     2700

aagggcggaa agtccaaatt gtaagcggcc gcgttgttaa acagaccaca acggtttccc     2760

tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata     2820

aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt     2880

gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     2940

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     3000

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     3060

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     3120

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     3180

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     3240

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     3300

gaaccacggg gacgtggttt tcctttgaaa aacgcgatcg caccgcgttg gccgattcat     3360

taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt     3420

aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt     3480

atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat     3540

tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa     3600

ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa     3660

tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga     3720

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct     3780

ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc     3840

tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg     3900

ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgggc     3960

cggcctcaca cctcccttac atcgaacagg gaatgcagct cgccgaacaa ttcaaacaga     4020

aggcaatcgg gttgctgcaa acagccacca agcaagcgga ggctgctgct cccgtggtgg     4080

aatccaagtg gcggaccctc gaagccttct gggcgaagca tatgtggaat ttcatcagcg     4140

ggatacaata tttagcaggc ttgtccactc tgcctggcaa ccccgcgata gcatcactga     4200

tggcattcac agcctctatc accagcccgc tcaccaccca acataccctc ctgtttaaca     4260

tcctgggggg atgggtggcc gcccaacttg ctcctcccag cgctgcttct gctttcgtag     4320

gcgccggcat cgctggagcg gctgttggca gcataggcct tgggacggtg cttgtggata     4380

ttttggcagg ttatggagca ggggtggcag gcgcgctcgt ggcctttaag gtcatgagcg     4440

gcgagatgcc ctccaccgag gacctggtta acctactccc tgctatcctc tcccctggcg     4500

ccctagtcgt cggggtcgtg tgcgcagcga tactgcgtcg gcacgtgggc ccaggggagg     4560

gggctgtgca gtggatgaac cggctgatag cgttcgcttc gcggggtaac cacgtctccc     4620

ccacgcacta tgtgcctgag agcgacgctg cagcacgtgt cactcagatc ctctctagtc     4680

ttaccatcac tcagctgctg aagaggcttc accagtggat caacgaggac tgctccacgc     4740

catgctccgg ctcgtggcta agagatgttt gggattggat atgcacggtg ttgactgatt     4800

tcaagacctg gctccagtcc aagctcctgc cgcgattgcc gggagtcccc ttcttctcat     4860

gtcaacgtgg gtacaaggga gtctggcggg gcgacggcat catgcaaacc acctgcccat     4920

gtggagcaca gatcaccgga catgtgaaaa acggttccat gaggatcgtg gggcctagga     4980

cctgtagtaa cacgtggcat ggaacattcc ccattaacgc gtacaccacg ggcccctgca     5040

cgccctcccc ggcgccaaat tattctaggg cgctgtggcg ggtggctgct gaggagtacg     5100

tggaggttac gcgggtgggg gatttccact acgtgacggg catgaccact gacaacgtaa     5160

agtgcccgtg tcaggttccg gcccccgaat tcttcacaga agtggatggg gtgcggttgc     5220

acaggtacgc tccagcgtgc aaacccctcc tacgggagga ggtcacattc ctggtcgggc     5280

tcaatcaata cctggttggg tcacagctcc catgcgagcc cgaaccggac gtagcagtgc     5340

tcacttccat gctcaccgac ccctcccaca ttacggcgga gacggctaag cgtaggctgg     5400

ccaggggatc tcccccctcc ttggccagct catcagctag ccagctgtct gcgccttcct     5460

tgaaggcaac atgcactacc cgtcatgact ccccggacgc tgacctcatc gaggccaacc     5520

tcctgtggcg gcaggagatg ggcgggaaca tcacccgcgt ggagtcagaa aataaggtag     5580

taattttgga ctctttcgag ccgctccaag cggaggagga tgagagggaa gtatccgttc     5640

cggcggagat cctgcggagg tccaggaaat tccctcgagc gatgcccata tgggcacgcc     5700

cggattacaa ccctccactg ttagagtcct ggaaggaccc ggactacgtc cctccagtgg     5760

tacacgggtg tccattgccg cctgccaagg cccctccgat accacctcca cggaggaaga     5820

ggacggttgt cctgtcagaa tctaccgtgt cttctgcctt ggcggagctc gccacaaaga     5880

ccttcggcag ctccgaatcg tcggccgtcg acagcggcac ggcaacggcc tctcctgacc     5940

agccctccga cgacggcgac gcgggatccg acgttgagtc gtactcctcc atgccccccc     6000

ttgaggggga gccgggggat cccgatctca gcgacgggtc ttggtctacc gtaagcgagg     6060

aggctagtga ggacgtcgtc tgctgctcga tgtcctacac atggacaggc gccctgatca     6120

cgccatgcgc tgcggaggaa accaagctgc ccatcaatgc actgagcaac tctttgctcc     6180

gtcaccacaa cttggtctat gctacaacat ctcgcagcgc aagcctgcgg cagaagaagg     6240

tcacctttga cagactgcag gtcctggacg accactaccg ggacgtgctc aaggagatga     6300

aggcgaaggc gtccacagtt aaggctaaac ttctatccgt ggaggaagcc tgtaagctga     6360

cgcccccaca ttcggccaga tctaaatttg gctatggggc aaaggacgtc cggaacctat     6420

ccagcaaggc cgttaaccac atccgctccg tgtggaagga cttgctggaa gacactgaga     6480

caccaattga caccaccatc atggcaaaaa atgaggtttt ctgcgtccaa ccagagaagg     6540

ggggccgcaa gccagctcgc cttatcgtat tcccagattt gggggttcgt gtgtgcgaga     6600

aaatggccct ttacgatgtg gtctccaccc tccctcaggc cgtgatgggc tcttcatacg     6660

gattccaata ctctcctgga cagcgggtcg agttcctggt gaatgcctgg aaagcgaaga     6720

aatgccctat gggcttcgca tatgacaccc gctgttttga ctcaacggtc actgagaatg     6780

acatccgtgt tgaggagtca atctaccaat gttgtgactt ggcccccgaa gccagacagg     6840

ccataaggtc gctcacagag cggctttaca tcgggggccc cctgactaat tctaaagggc     6900

agaactgcgg ctatcgccgg tgccgcgcga gcggtgtact gacgaccagc tgcggtaata     6960

ccctcacatg ttacttgaag gccgctgcgg cctgtcgagc tgcgaagctc caggactgca     7020

cgatgctcgt atgcggagac gaccttgtcg ttatctgtga aagcgcgggg acccaagagg     7080

acgaggcgag cctacgggcc ttcacggagg ctatgactag atactctgcc ccccctgggg     7140

acccgcccaa accagaatac gacttggagt tgataacatc atgctcctcc aatgtgtcag     7200

tcgcgcacga tgcatctggc aaaagggtgt actatctcac ccgtgacccc accacccccc     7260

ttgcgcgggc tgcgtgggag acagctagac acactccagt caattcctgg ctaggcaaca     7320

tcatcatgta tgcgcccacc ttgtgggcaa ggatgatcct gatgactcat ttcttctcca     7380

tccttctagc tcaggaacaa cttgaaaaag ccctagattg tcagatctac ggggcctgtt     7440

actccattga gccacttgac ctacctcaga tcattcaacg actccatggc cttagcgcat     7500

tttcactcca tagttactct ccaggtgaga tcaatagggt ggcttcatgc ctcaggaaac     7560

ttggggtacc gcccttgcga gtctggagac atcgggccag aagtgtccgc gctaggctac     7620

tgtcccaggg ggggagggct gccacttgtg gcaagtacct cttcaactgg gcagtaagga     7680

ccaagctcaa actcactcca atcccggctg cgtcccagtt ggatttatcc agctggttcg     7740

ttgctggtta cagcggggga gacatatatc acagcctgtc tcgtgcccga ccccgctggt     7800

tcatgtggtg cctactccta ctttctgtag gggtaggcat ctatctactc cccaaccgat     7860

gaacggggag ctaaacactc caggccaata ggccatcctg tttttttccc tttttttttt     7920

tctttttttt tttttttttt tttttttttt ttttttctcc tttttttttc ctcttttttt     7980

ccttttcttt cctttggtgg ctccatctta gccctagtca cggctagctg tgaaaggtcc     8040

gtgagccgct tgactgcaga gagtgctgat actggcctct ctgcagatca agtactacta     8100

gtagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct     8160

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     8220

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     8280

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     8340

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     8400

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     8460

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     8520

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     8580

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     8640

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     8700

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     8760

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     8820

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     8880

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     8940

acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga     9000

tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt     9060

ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt     9120

catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat     9180

ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag     9240

caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct     9300

ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt     9360

tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg     9420

cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca     9480

aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt     9540

tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat     9600

gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac     9660

cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa     9720

aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt     9780

tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt     9840

tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa     9900

gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt     9960

atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa    10020

taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accatt        10076


<210>  6
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector FspAI sense primer

<400>  6
gtctccggga gctgggtgcg catcacacct cccttaca                               38


<210>  7
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector FspAI anti-sense primer

<400>  7
tgtaagggag gtgtgatgcg cacccagctc ccggagac                               38


<210>  8
<211>  10076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSC_1b_NS3/4A/lacZ_AsiSI_FspA

<400>  8
cctgcaggta atacgactca ctatagccag cccccgattg ggggcgacac tccaccatag       60

atcactcccc tgtgaggaac tactgtcttc acgcagaaag cgtctagcca tggcgttagt      120

atgagtgtcg tgcagcctcc aggacccccc ctcccgggag agccatagtg gtctgcggaa      180

ccggtgagta caccggaatt gccaggacga ccgggtcctt tcttggatca acccgctcaa      240

tgcctggaga tttgggcgtg cccccgcgag actgctagcc gagtagtgtt gggtcgcgaa      300

aggccttgtg gtactgcctg atagggtgct tgcgagtgcc ccgggaggtc tcgtagaccg      360

tgcaccgttt aaacccccgt gctgctggaa gtcgatttcg cttagggtaa ccgtggacct      420

cgaaaacaga cgcacaaaac caagttcaat agaagggggt acaaaccagt accaccacga      480

acaagcactt ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg      540

gatccgttat ccgcttatgt acttcgagaa gcccagtacc acctcggaat cttcgatgcg      600

ttgcgctcag cactcaaccc cagagtgtag cttaggctga tgagtctgga catccctcac      660

cggtgacggt ggtccaggct gcgttggcgg cctacctatg gctaacgcca tgggacgcta      720

gttgtgaaca aggtgtgaag agcctattga gctacataag aatcctccgg cccctgaatg      780

cggctaatcc caacctcgga gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg      840

caagtccgtg gcggaaccga ctactttggg tgtccgtgtt tccttttatt ttattgtggc      900

tgcttatggt gacaatcaca gattgttatc ataaagcgaa ttggattggc catccggtga      960

aagtgagact cattatctat ctgtttgctg gatccgctcc attgagtgtg tttactctaa     1020

gtacaatttc aacagttatt tcaatcagac aattgtatca taatggcggg cccagaagac     1080

gccaaaaaca taaaggaagg cccggcgcca ttctatcctc ttgaggatgg aaccgctgga     1140

gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca     1200

gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg     1260

gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa     1320

aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg     1380

cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct     1440

accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta     1500

ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg     1560

atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca     1620

gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg     1680

ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga     1740

gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc     1800

catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc     1860

ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa     1920

agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa     1980

tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc     2040

ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact     2100

gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt     2160

aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc     2220

gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac     2280

aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata     2340

gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa     2400

tacaaaggtt atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac     2460

atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc     2520

gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc     2580

agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg     2640

aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag     2700

aagggcggaa agtccaaatt gtaagcggcc gcgttgttaa acagaccaca acggtttccc     2760

tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata     2820

aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt     2880

gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     2940

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     3000

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     3060

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     3120

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     3180

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     3240

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     3300

gaaccacggg gacgtggttt tcctttgaaa aacgcgatcg caccgcgttg gccgattcat     3360

taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt     3420

aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt     3480

atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat     3540

tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa     3600

ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa     3660

tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga     3720

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct     3780

ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc     3840

tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg     3900

ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgggt     3960

gcgcatcaca cctcccttac atcgaacagg gaatgcagct cgccgaacaa ttcaaacaga     4020

aggcaatcgg gttgctgcaa acagccacca agcaagcgga ggctgctgct cccgtggtgg     4080

aatccaagtg gcggaccctc gaagccttct gggcgaagca tatgtggaat ttcatcagcg     4140

ggatacaata tttagcaggc ttgtccactc tgcctggcaa ccccgcgata gcatcactga     4200

tggcattcac agcctctatc accagcccgc tcaccaccca acataccctc ctgtttaaca     4260

tcctgggggg atgggtggcc gcccaacttg ctcctcccag cgctgcttct gctttcgtag     4320

gcgccggcat cgctggagcg gctgttggca gcataggcct tgggacggtg cttgtggata     4380

ttttggcagg ttatggagca ggggtggcag gcgcgctcgt ggcctttaag gtcatgagcg     4440

gcgagatgcc ctccaccgag gacctggtta acctactccc tgctatcctc tcccctggcg     4500

ccctagtcgt cggggtcgtg tgcgcagcga tactgcgtcg gcacgtgggc ccaggggagg     4560

gggctgtgca gtggatgaac cggctgatag cgttcgcttc gcggggtaac cacgtctccc     4620

ccacgcacta tgtgcctgag agcgacgctg cagcacgtgt cactcagatc ctctctagtc     4680

ttaccatcac tcagctgctg aagaggcttc accagtggat caacgaggac tgctccacgc     4740

catgctccgg ctcgtggcta agagatgttt gggattggat atgcacggtg ttgactgatt     4800

tcaagacctg gctccagtcc aagctcctgc cgcgattgcc gggagtcccc ttcttctcat     4860

gtcaacgtgg gtacaaggga gtctggcggg gcgacggcat catgcaaacc acctgcccat     4920

gtggagcaca gatcaccgga catgtgaaaa acggttccat gaggatcgtg gggcctagga     4980

cctgtagtaa cacgtggcat ggaacattcc ccattaacgc gtacaccacg ggcccctgca     5040

cgccctcccc ggcgccaaat tattctaggg cgctgtggcg ggtggctgct gaggagtacg     5100

tggaggttac gcgggtgggg gatttccact acgtgacggg catgaccact gacaacgtaa     5160

agtgcccgtg tcaggttccg gcccccgaat tcttcacaga agtggatggg gtgcggttgc     5220

acaggtacgc tccagcgtgc aaacccctcc tacgggagga ggtcacattc ctggtcgggc     5280

tcaatcaata cctggttggg tcacagctcc catgcgagcc cgaaccggac gtagcagtgc     5340

tcacttccat gctcaccgac ccctcccaca ttacggcgga gacggctaag cgtaggctgg     5400

ccaggggatc tcccccctcc ttggccagct catcagctag ccagctgtct gcgccttcct     5460

tgaaggcaac atgcactacc cgtcatgact ccccggacgc tgacctcatc gaggccaacc     5520

tcctgtggcg gcaggagatg ggcgggaaca tcacccgcgt ggagtcagaa aataaggtag     5580

taattttgga ctctttcgag ccgctccaag cggaggagga tgagagggaa gtatccgttc     5640

cggcggagat cctgcggagg tccaggaaat tccctcgagc gatgcccata tgggcacgcc     5700

cggattacaa ccctccactg ttagagtcct ggaaggaccc ggactacgtc cctccagtgg     5760

tacacgggtg tccattgccg cctgccaagg cccctccgat accacctcca cggaggaaga     5820

ggacggttgt cctgtcagaa tctaccgtgt cttctgcctt ggcggagctc gccacaaaga     5880

ccttcggcag ctccgaatcg tcggccgtcg acagcggcac ggcaacggcc tctcctgacc     5940

agccctccga cgacggcgac gcgggatccg acgttgagtc gtactcctcc atgccccccc     6000

ttgaggggga gccgggggat cccgatctca gcgacgggtc ttggtctacc gtaagcgagg     6060

aggctagtga ggacgtcgtc tgctgctcga tgtcctacac atggacaggc gccctgatca     6120

cgccatgcgc tgcggaggaa accaagctgc ccatcaatgc actgagcaac tctttgctcc     6180

gtcaccacaa cttggtctat gctacaacat ctcgcagcgc aagcctgcgg cagaagaagg     6240

tcacctttga cagactgcag gtcctggacg accactaccg ggacgtgctc aaggagatga     6300

aggcgaaggc gtccacagtt aaggctaaac ttctatccgt ggaggaagcc tgtaagctga     6360

cgcccccaca ttcggccaga tctaaatttg gctatggggc aaaggacgtc cggaacctat     6420

ccagcaaggc cgttaaccac atccgctccg tgtggaagga cttgctggaa gacactgaga     6480

caccaattga caccaccatc atggcaaaaa atgaggtttt ctgcgtccaa ccagagaagg     6540

ggggccgcaa gccagctcgc cttatcgtat tcccagattt gggggttcgt gtgtgcgaga     6600

aaatggccct ttacgatgtg gtctccaccc tccctcaggc cgtgatgggc tcttcatacg     6660

gattccaata ctctcctgga cagcgggtcg agttcctggt gaatgcctgg aaagcgaaga     6720

aatgccctat gggcttcgca tatgacaccc gctgttttga ctcaacggtc actgagaatg     6780

acatccgtgt tgaggagtca atctaccaat gttgtgactt ggcccccgaa gccagacagg     6840

ccataaggtc gctcacagag cggctttaca tcgggggccc cctgactaat tctaaagggc     6900

agaactgcgg ctatcgccgg tgccgcgcga gcggtgtact gacgaccagc tgcggtaata     6960

ccctcacatg ttacttgaag gccgctgcgg cctgtcgagc tgcgaagctc caggactgca     7020

cgatgctcgt atgcggagac gaccttgtcg ttatctgtga aagcgcgggg acccaagagg     7080

acgaggcgag cctacgggcc ttcacggagg ctatgactag atactctgcc ccccctgggg     7140

acccgcccaa accagaatac gacttggagt tgataacatc atgctcctcc aatgtgtcag     7200

tcgcgcacga tgcatctggc aaaagggtgt actatctcac ccgtgacccc accacccccc     7260

ttgcgcgggc tgcgtgggag acagctagac acactccagt caattcctgg ctaggcaaca     7320

tcatcatgta tgcgcccacc ttgtgggcaa ggatgatcct gatgactcat ttcttctcca     7380

tccttctagc tcaggaacaa cttgaaaaag ccctagattg tcagatctac ggggcctgtt     7440

actccattga gccacttgac ctacctcaga tcattcaacg actccatggc cttagcgcat     7500

tttcactcca tagttactct ccaggtgaga tcaatagggt ggcttcatgc ctcaggaaac     7560

ttggggtacc gcccttgcga gtctggagac atcgggccag aagtgtccgc gctaggctac     7620

tgtcccaggg ggggagggct gccacttgtg gcaagtacct cttcaactgg gcagtaagga     7680

ccaagctcaa actcactcca atcccggctg cgtcccagtt ggatttatcc agctggttcg     7740

ttgctggtta cagcggggga gacatatatc acagcctgtc tcgtgcccga ccccgctggt     7800

tcatgtggtg cctactccta ctttctgtag gggtaggcat ctatctactc cccaaccgat     7860

gaacggggag ctaaacactc caggccaata ggccatcctg tttttttccc tttttttttt     7920

tctttttttt tttttttttt tttttttttt ttttttctcc tttttttttc ctcttttttt     7980

ccttttcttt cctttggtgg ctccatctta gccctagtca cggctagctg tgaaaggtcc     8040

gtgagccgct tgactgcaga gagtgctgat actggcctct ctgcagatca agtactacta     8100

gtagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct     8160

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     8220

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     8280

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     8340

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     8400

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     8460

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     8520

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     8580

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     8640

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     8700

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     8760

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     8820

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     8880

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     8940

acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga     9000

tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt     9060

ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt     9120

catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat     9180

ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag     9240

caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct     9300

ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt     9360

tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg     9420

cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca     9480

aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt     9540

tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat     9600

gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac     9660

cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa     9720

aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt     9780

tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt     9840

tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa     9900

gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt     9960

atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa    10020

taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accatt        10076


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse transcription anti-sense primer


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  where s is g or c

<400>  9
accaggtcct csgtggagg                                                    19


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1st round PCR sense primer

<400>  10
gagaccaaga tcatcacc                                                     18


<210>  11
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2nd round PCR sense primer

<400>  11
ccaccacggg agcagc                                                       16


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2nd round PCR sense primer

<400>  12
ggcagacacc gcggcgtgtg g                                                 21


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2nd round PCR anti-sense primer

<400>  13
ttccacatgt gcttcgccc                                                    19


<210>  14
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer AsiSI

<400>  14
ctgtctgtct gcgatcgcac catggcgcct attacggcct actc                        44


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer FspAI

<400>  15
agggaggtgt gatgcgcact cttccatctc                                        30


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer FseI

<400>  16
cgcactcttc catctcatcg aactc                                             25


