                         SEQUENCE LISTING

<110>  Bayer HealthCare LLC
       Brooks, Alan
       Patel, Chandra
       Jiang, Xiaoqiao
       Gritzan, Uwe
       Apeler, Heiner
       Wang, Jun
 
<120>  MODIFIED FACTOR IX POLYPEPTIDES AND USES THEREOF

<130>  BHC 10 5 004

<150>  US 61/230,551
<151>  2009-07-31

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  415
<212>  PRT
<213>  Homo sapiens

<400>  1

Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      


Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          


Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              


Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  


Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  


Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      


Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         


Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             


Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 


Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 


Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     


Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         


Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             


Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 


Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 


Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     


His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         


Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             


Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 


Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 


Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     


Leu Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         


Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             


His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 


Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 


Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
                405                 410                 415 


<210>  2
<211>  415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FIX variants


<220>
<221>  MISC_FEATURE
<222>  (85)..(85)
<223>  X is selected from D, F, G, H, I, M, N, R, S, W, and Y

<220>
<221>  MISC_FEATURE
<222>  (86)..(86)
<223>  X is selected from A, D, E, G, H, I, L, M, N, P, Q, R, S, T, and 
       V

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  X is selected from F, I, K, M, R, T, V, and W

<220>
<221>  MISC_FEATURE
<222>  (338)..(338)
<223>  X A, F, I, L, M, R, S, T, V, and W

<220>
<221>  MISC_FEATURE
<222>  (410)..(410)
<223>  X is selected from E, N, and Q

<400>  2

Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      


Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          


Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              


Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  


Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  


Asn Cys Glu Leu Xaa Xaa Xaa Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      


Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         


Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             


Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 


Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 


Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     


Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         


Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             


Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 


Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 


Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     


His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         


Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             


Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 


Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 


Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     


Leu Xaa Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         


Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             


His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 


Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 


Val Ser Arg Tyr Val Asn Trp Ile Lys Xaa Lys Thr Lys Leu Thr 
                405                 410                 415 


<210>  3
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIXF1 5' primer

<400>  3
atcataagct tgccaccatg cagcgcgtga acatg                                  35


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIXR3 3' primer

<400>  4
atcataagct tgattagtta gtgagaggcc ctg                                    33


<210>  5
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86A primer

<400>  5
aggaaagaac tgtgaattag atgccacatg taacattaag aatggca                     47


<210>  6
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86A primer

<400>  6
tgccattctt aatgttacat gtggcatcta attcacagtt ctttcct                     47


<210>  7
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86P primer

<400>  7
ggaaagaact gtgaattaga tcccacatgt aacattaaga atggcag                     47


<210>  8
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86P primer

<400>  8
ctgccattct taatgttaca tgtgggatct aattcacagt tctttcc                     47


<210>  9
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86E primer

<400>  9
ggaaagaact gtgaattaga tgagacctgt aacattaaga atggcag                     47


<210>  10
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86E primer

<400>  10
ctgccattct taatgttaca ggtctcatct aattcacagt tctttcc                     47


<210>  11
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86S primer

<400>  11
ggaaagaact gtgaattaga tagcacctgt aacattaaga atggcag                     47


<210>  12
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86S primer

<400>  12
ctgccattct taatgttaca ggtgctatct aattcacagt tctttcc                     47


<210>  13
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86I primer

<400>  13
ggaaagaact gtgaattaga tatcacctgt aacattaaga atggcag                     47


<210>  14
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86I primer

<400>  14
ctgccattct taatgttaca ggtgatatct aattcacagt tctttcc                     47


<210>  15
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86R primer

<400>  15
ggaaagaact gtgaattaga tagaacctgt aacattaaga atggcag                     47


<210>  16
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86R primer

<400>  16
ctgccattct taatgttaca ggttctatct aattcacagt tctttcc                     47


<210>  17
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86Q primer

<400>  17
ggaaagaact gtgaattaga tcagacatgt aacattaaga atggcag                     47


<210>  18
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86Q primer

<400>  18
ctgccattct taatgttaca tgtctgatct aattcacagt tctttcc                     47


<210>  19
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86T primer

<400>  19
ggaaagaact gtgaattaga taccacctgt aacattaaga atggcag                     47


<210>  20
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86T primer

<400>  20
ctgccattct taatgttaca ggtggtatct aattcacagt tctttcc                     47


<210>  21
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86D primer

<400>  21
ggaaagaact gtgaattaga tgacacctgt aacattaaga atggcag                     47


<210>  22
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86D primer

<400>  22
ctgccattct taatgttaca ggtgtcatct aattcacagt tctttcc                     47


<210>  23
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86H primer

<400>  23
ggaaagaact gtgaattaga tcacacctgt aacattaaga atggcag                     47


<210>  24
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86H primer

<400>  24
ctgccattct taatgttaca ggtgtgatct aattcacagt tctttcc                     47


<210>  25
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86N primer

<400>  25
ggaaagaact gtgaattaga taacacctgt aacattaaga atggcag                     47


<210>  26
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86N primer

<400>  26
ctgccattct taatgttaca ggtgttatct aattcacagt tctttcc                     47


<210>  27
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86L primer

<400>  27
ggaaagaact gtgaattaga tctgacatgt aacattaaga atggcag                     47


<210>  28
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86L primer

<400>  28
ctgccattct taatgttaca tgtcagatct aattcacagt tctttcc                     47


<210>  29
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86M primer

<400>  29
ggaaagaact gtgaattaga tatgacctgt aacattaaga atggcag                     47


<210>  30
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86M primer

<400>  30
ctgccattct taatgttaca ggtcatatct aattcacagt tctttcc                     47


<210>  31
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86Y primer

<400>  31
ggaaagaact gtgaattaga ttacacctgt aacattaaga atggcag                     47


<210>  32
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86Y primer

<400>  32
ctgccattct taatgttaca ggtgtaatct aattcacagt tctttcc                     47


<210>  33
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86K primer

<400>  33
ggaaagaact gtgaattaga taagacatgt aacattaaga atggcag                     47


<210>  34
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86K primer

<400>  34
ctgccattct taatgttaca tgtcttatct aattcacagt tctttcc                     47


<210>  35
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86F primer

<400>  35
ggaaagaact gtgaattaga tttcacctgt aacattaaga atggcag                     47


<210>  36
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86F primer

<400>  36
ctgccattct taatgttaca ggtgaaatct aattcacagt tctttcc                     47


<210>  37
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86C primer

<400>  37
ggaaagaact gtgaattaga ttgcacctgt aacattaaga atggcag                     47


<210>  38
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86C primer

<400>  38
ctgccattct taatgttaca ggtgcaatct aattcacagt tctttc                      46


<210>  39
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86W primer

<400>  39
ggaaagaact gtgaattaga ttggacctgt aacattaaga atggcag                     47


<210>  40
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86W primer

<400>  40
ctgccattct taatgttaca ggtccaatct aattcacagt tctttcc                     47


<210>  41
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86G primer

<400>  41
gaaggaaaga actgtgaatt agatggcacc tgtaacatta agaatggcag atgcg            55


<210>  42
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V86G primer

<400>  42
cgcatctgcc attcttaatg ttacaggtgc catctaattc acagttcttt ccttc            55


<210>  43
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E410N primer

<400>  43
tcccggtatg tcaactggat taagaacaaa acaaagctca cttaatgaaa g                51


<210>  44
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E410N primer

<400>  44
ctttcattaa gtgagctttg ttttgttctt aatccagttg acataccggg a                51


<210>  45
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E410Q primer

<400>  45
tcccggtatg tcaactggat taagcagaaa acaaagctca cttaatgaaa g                51


<210>  46
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E410Q primer

<400>  46
ctttcattaa gtgagctttg ttttctgctt aatccagttg acataccggg a                51


<210>  47
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  47
ctgtttttcc tgatgtggac tacgtagcct ctactgaagc tgaaaccatt ct               52


<210>  48
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  48
gaagctgaaa ccattctaga tgccatcact caaagcaccc aatc                        44


<210>  49
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  49
cttgttgacc gagccacatg ccttgcatct acaaagttca ccatc                       45


<210>  50
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  50
cttgttgacc gagccacatg ccttctgtct acaaagttca ccatc                       45


<210>  51
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  51
gaccgagcca catgccttgt gtctacaaag ttcaccatc                              39


<210>  52
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  52
gttgaccgag ccacatgcct tatctctaca aagttcacca tctataac                    48


<210>  53
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  53
gttgaccgag ccacatgcct tttctctaca aagttcacca tctataac                    48


<210>  54
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  54
cttgttgacc gagccacatg cctttggtct acaaagttca ccatc                       45


<210>  55
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  55
cacttgttga ccgagccaca tgccttatgt ctacaaagtt caccatc                     47


<210>  56
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  56
cttgttgacc gagccacatg ccttagctct acaaagttca ccatc                       45


<210>  57
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  57
gttgaccgag ccacatgcct tacctctaca aagttcacca tc                          42


