                         SEQUENCE LISTING

<110>  METABOLIC EXPLORER
       VOELKER, FRANCOIS
       DUMON-SEIGNOVERT, LAURENCE
       SOUCAILLE, PHILIPPE
 
<120>  Mutant methylglyoxal synthase (MGS) for the production of a biochemical
       by fermentation

<130>  D27288

<150>  EP09166815.2
<151>  2009-07-30

<150>  US 61/230,076
<151>  2009-07-30

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  152
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Glu Leu Thr Thr Arg Thr Leu Pro Ala Arg Lys His Ile Ala Leu 
1               5                   10                  15      


Val Ala His Asp His Cys Lys Gln Met Leu Met Ser Trp Val Glu Arg 
            20                  25                  30          


His Gln Pro Leu Leu Glu Gln His Val Leu Tyr Ala Thr Gly Thr Thr 
        35                  40                  45              


Gly Asn Leu Ile Ser Arg Ala Thr Gly Met Asn Val Asn Ala Met Leu 
    50                  55                  60                  


Ser Gly Pro Met Gly Gly Asp Gln Gln Val Gly Ala Leu Ile Ser Glu 
65                  70                  75                  80  


Gly Lys Ile Asp Val Leu Ile Phe Phe Trp Asp Pro Leu Asn Val Val 
                85                  90                  95      


Pro His Asp Pro Asp Val Lys Ala Leu Leu Arg Leu Ala Thr Val Trp 
            100                 105                 110         


Asn Ile Pro Val Ala Thr Asn Val Ala Thr Ala Asp Phe Ile Ile Gln 
        115                 120                 125             


Ser Pro His Phe Asn Asp Ala Val Asp Ile Leu Ile Pro Asp Tyr Gln 
    130                 135                 140                 


Arg Tyr Leu Ala Asp Arg Leu Lys 
145                 150         


<210>  2
<211>  152
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Glu Leu Thr Thr Arg Thr Leu Pro Ala Arg Lys His Ile Ala Leu 
1               5                   10                  15      


Val Ala His Asp Gln Cys Lys Gln Met Leu Met Ser Trp Val Glu Arg 
            20                  25                  30          


His Gln Pro Leu Leu Glu Gln His Val Leu Tyr Ala Thr Gly Thr Thr 
        35                  40                  45              


Gly Asn Leu Ile Ser Arg Ala Thr Gly Met Asn Val Asn Ala Met Leu 
    50                  55                  60                  


Ser Gly Pro Met Gly Gly Asp Gln Gln Val Gly Ala Leu Ile Ser Glu 
65                  70                  75                  80  


Gly Lys Ile Asp Val Leu Ile Phe Phe Trp Asp Pro Leu Asn Ala Val 
                85                  90                  95      


Pro His Asp Pro Asp Val Lys Ala Leu Leu Arg Leu Ala Thr Val Trp 
            100                 105                 110         


Asn Ile Pro Val Ala Thr Asn Val Ala Thr Ala Asp Phe Ile Ile Gln 
        115                 120                 125             


Ser Pro His Phe Asn Asp Ala Val Asp Ile Leu Ile Pro Asp Tyr Gln 
    130                 135                 140                 


Arg Tyr Leu Ala Asp Arg Leu Lys 
145                 150         


<210>  3
<211>  152
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Glu Leu Thr Thr Arg Thr Leu Pro Ala Arg Lys His Ile Ala Leu 
1               5                   10                  15      


Val Ala His Asp His Cys Lys Gln Met Leu Met Ser Trp Val Glu Arg 
            20                  25                  30          


His Gln Pro Leu Leu Glu Gln His Val Leu Tyr Ala Thr Gly Thr Thr 
        35                  40                  45              


Gly Asn Leu Ile Ser Arg Ala Thr Gly Met Asn Val Asn Ala Met Leu 
    50                  55                  60                  


Ser Gly Pro Met Gly Gly Asp Gln Gln Val Gly Ala Leu Ile Ser Glu 
65                  70                  75                  80  


Gly Lys Ile Asp Val Leu Ile Phe Phe Trp Asp Pro Leu Asn Ala Val 
                85                  90                  95      


Pro His Asp Pro Asp Val Lys Ala Leu Leu Arg Leu Ala Thr Val Trp 
            100                 105                 110         


Asn Ile Pro Leu Ala Thr Asn Val Ala Thr Ala Asp Phe Ile Ile Gln 
        115                 120                 125             


Ser Pro His Phe Asn Asp Ala Val Asp Ile Leu Ile Pro Asp Tyr Gln 
    130                 135                 140                 


Arg Tyr Leu Ala Asp Arg Leu Lys 
145                 150         


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  4
caccatggaa ctgacgactc gca                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  5
ttacttcaga cggtccgcga gat                                               23


<210>  6
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  6
ttctgggatc cactaaatgt cgtgccgcac gatcctgacg tcaaagcc                    48


<210>  7
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  7
ggctttgacg tcaggatcgt gcggcacgac atttagtgga tcccagaa                    48


<210>  8
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  8
gcgacggtat ggaacattcc gctcgcgacc aacgtggcaa cg                          42


<210>  9
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  9
cgttgccacg ttggtcgcga gcggaatgtt ccataccgtc gc                          42


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  10
ctggtggcac acgatcaatg caaacagatg ctgatgagct gggtg                       45


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  11
cacccagctc atcagcatct gtttgcattg atcgtgtgcc accag                       45


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  12
ccgacagtaa gacgggtaag cctg                                              24


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  13
agcttagtaa agccctcgct ag                                                22


<210>  14
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  14
catgggctag ctacgtatta attaaagatc tcctagggag ctcaccggtt aaaaataaga       60

gttaccttaa atggtaactc ttattttttt aggcgcgcca                            100


<210>  15
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  15
agcttggcgc gcctaaaaaa ataagagtta ccatttaagg taactcttat ttttaaccgg       60

tgagctccct aggagatctt taattaatac gtagctagcc                            100


<210>  16
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  16
cgatgcacgt catgaacaac tttaatctgc acaccccaac ccg                         43


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  17
ctagctagct tagcgggcgg cttcgtata                                         29


<210>  18
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  18
ggttcagaat ccaacgcaga agcggtgata tcccgtaaaa ccacaggc                    48


<210>  19
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  19
gcctgtggtt ttacgggata tcaccgcttc tgcgttggat tctgaacc                    48


<210>  20
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  20
gacaccaaaa tcgtcgctgg cacacctgca cgtctgctag cggcg                       45


<210>  21
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  21
cgccgctagc agacgtgcag gtgtgccagc gacgattttg gtgtc                       45


<210>  22
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  22
gttaactacg gatgtacatt atggaactga cgactcgcac tttacctgcg cggaaacata       60

ttgcgctggt ggcacacgat caggcctggt gatgatggcg ggatc                      105


<210>  23
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  23
gggaaattaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt       60

gatgccgttc cacccagctc atcagcatct gtttgcattc agaagaactc gtcaagaagg      120


<210>  24
<211>  100
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Oligonucleotide

<400>  24
tccagtcgcc gcatttcaac gacgcggtcg atattctgat ccccgattat cagcgttatc       60

tcgcggaccg tctgaagtaa tgtaggctgg agctgcttcg                            100


<210>  25
<211>  100
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Oligonucleotide

<400>  25
tgtggaaata ctgaaaaatc tggatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg       60

cgtttgccac ctgtgcaata catatgaata tcctccttag                            100


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Ptrc01 promoter

<400>  26
gagctgttga ctattaatca tccggctcga ataatgtgtg g                           41


<210>  27
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  27
atgcttgctg tcagaagatt aacaagatac acattcctta gtttatcatt atcaatactc       60

g                                                                       61


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  28
atcctcgaaa ctaagttctt ggt                                               23


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  29
aaacaccaga acttagtttc gaactagttt attcccactc tt                          42


<210>  30
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  30
tgaaatataa ataacgttct taatactaac ataactataa actagtatgg accgcattat       60

tc                                                                      62


<210>  31
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  31
cctggatgta tttacccggt gattgaataa tgcggtccat actagtttat agttatgtta       60

gtatta                                                                  66


<210>  32
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  32
gaggcaagct aaacagatct ctagacctag gtacccgccg gcaaattaaa gccttcgagc       60


<210>  33
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  33
gcttgagaag gttttgggac gctcgaaggc tttaatttgc taggtctaga gatctgttta       60

gc                                                                      62


<210>  34
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  34
ctattcgtca tcgatgtcta gctcttcaat catctccggt ccactagtgg atctgatatc       60

acct                                                                    64


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  35
atgcttgctg tcagaagatt                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  36
tagtatggac cgcattattc                                                   20


<210>  37
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  37
atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc agctggagct catagcttca       60


<210>  38
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  38
tgcgggttgg ggtgtgcaga ttaaagttgt tcatactagt ggatccacta gttctagaaa       60


<210>  39
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  39
catagcaatc taatctaagt tttctagaac tagtggatcc actagtatga acaactttaa       60


<210>  40
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  40
tgaaatataa ataacgttct taatactaac ataactataa actagtttag cgggcggctt       60


<210>  41
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  41
tgtcagccgc cgtatatacg aagccgcccg ctaaactagt ttatagttat gttagtatta       60


<210>  42
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  42
ctccatgtcg ctggccgggt gacccggcgg ggacgaggca gcaaattaaa gccttcgagc       60


<210>  43
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  43
gcttgagaag gttttgggac gctcgaaggc tttaatttgc tgcctcgtcc ccgccgggtc       60


<210>  44
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  44
ttaagcaagg attttcttaa cttcttcggc gacagcatca cagtatagcg accagcattc       60


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  45
atgtctgccc ctaagaagat c                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  46
actagtttag cgggcggctt                                                   20


<210>  47
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  47
atgtcgaaag ctacatataa ggaacgtgct gctactcatc gccagatctg tttagcttgc       60


<210>  48
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  48
gtgcgagtcg tcagttccat aatacgcaaa ccgcctctcc                             40


<210>  49
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  49
ggagaggcgg tttgcgtatt atggaactga cgactcgcac                             40


<210>  50
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  50
ttagttttgc tggccgcatc ttctcaaata tgcttccctt tacttcagac ggtccgcgag       60


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  51
ggaactgacg actcgcac                                                     18


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  52
ttagtttcta gagttgatga                                                   20


