                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Preparation of 1,4-diaminobutane

<130>  27102-WO-PCT

<160>  26    

<170>  PatentIn version 3.3

<210>  1
<211>  1371
<212>  DNA
<213>  Pseudomonas aeruginosa


<220>
<221>  CDS
<222>  (1)..(1371)
<223>  amino transferase gi9946143

<400>  1
atg aac agc caa atc acc aac gcc aag acc cgt gag tgg cag gcg ttg       48
Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu         
1               5                   10                  15              

agc cgc gac cac cat ctg ccg ccg ttc acc gac tac aag cag ttg aac       96
Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn         
            20                  25                  30                  

gag aag ggc gcg cgg atc atc acc aag gcc gaa ggc gtc tat atc tgg      144
Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp         
        35                  40                  45                      

gac agc gag ggc aac aag atc ctc gat gcg atg gcc ggc ctc tgg tgc      192
Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys         
    50                  55                  60                          

gtc aac gtc ggc tac ggc cgc gag gag ctg gtc cag gcc gcc acc cgg      240
Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg         
65                  70                  75                  80          

cag atg cgc gag ttg ccg ttc tac aac ctg ttc ttc cag acc gcc cac      288
Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His         
                85                  90                  95              

ccg ccg gtg gtc gag ctg gcc aag gcg atc gcc gac gtc gct ccg gaa      336
Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu         
            100                 105                 110                 

ggc atg aac cac gtg ttc ttc acc ggc tcc ggc tcc gag gcc aac gac      384
Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp         
        115                 120                 125                     

acc gtg ctg cgt atg gtc cgc cac tat tgg gcg acc aag ggc cag ccg      432
Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro         
    130                 135                 140                         

cag aag aaa gtg gtg atc ggc cgc tgg aac ggc tac cac ggc tcc acc      480
Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr         
145                 150                 155                 160         

gtc gcc ggc gtc agc ctg ggc ggc atg aag gcg ttg cat gag cag ggt      528
Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly         
                165                 170                 175             

gat ttc ccc atc ccg ggc atc gtc cac atc gcc cag ccc tac tgg tac      576
Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr         
            180                 185                 190                 

ggc gag ggc ggc gac atg tcg ccg gac gag ttc ggc gtc tgg gcc gcc      624
Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala         
        195                 200                 205                     

gag cag ttg gag aag aag att ctc gaa gtg ggc gag gaa aac gtc gcc      672
Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala         
    210                 215                 220                         

gcc ttc atc gcc gag ccg atc cag ggc gcc ggc ggc gtg atc gtc ccg      720
Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro         
225                 230                 235                 240         

ccg gac acc tac tgg ccg aag atc cgc gag atc ctc gcc aag tac gac      768
Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp         
                245                 250                 255             

atc ctg ttc atc gcc gac gaa gtg atc tgc ggc ttc ggc cgt acc ggc      816
Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly         
            260                 265                 270                 

gag tgg ttc ggc agc cag tac tac ggc aac gcc ccg gac ctg atg ccg      864
Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro         
        275                 280                 285                     

atc gcc aag ggc ctc acc tcc ggc tac atc ccc atg ggc ggg gtg gtg      912
Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val         
    290                 295                 300                         

gtg cgc gac gag atc gtc gaa gtg ctc aac cag ggc ggc gag ttc tac      960
Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr         
305                 310                 315                 320         

cac ggc ttc acc tat tcc ggt cac ccg gtg gcg gcc gcc gtg gcc ctg     1008
His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu         
                325                 330                 335             

gag aac atc cgc atc ctg cgc gaa gag aag atc atc gag aag gtg aag     1056
Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys         
            340                 345                 350                 

gcg gaa acg gca ccg tat ttg cag aaa cgc tgg cag gag ctg gcc gac     1104
Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp         
        355                 360                 365                     

cac ccg ttg gtg ggc gaa gcg cgc ggg gtc ggc atg gtc gcc gcc ctg     1152
His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu         
    370                 375                 380                         

gag ctg gtc aag aac aag aag acc cgc gag cgt ttc acc gac aag ggc     1200
Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly         
385                 390                 395                 400         

gtc ggg atg ctg tgc cgg gaa cat tgt ttc cgc aac ggt ttg atc atg     1248
Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met         
                405                 410                 415             

cgc gcg gtg ggc gac act atg att atc tcg ccg ccg ctg gtg atc gat     1296
Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp         
            420                 425                 430                 

ccg tcg cag atc gat gag ttg atc acc ctg gcg cgc aag tgc ctc gat     1344
Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp         
        435                 440                 445                     

cag acc gcc gcc gcc gtc ctg gct tga                                 1371
Gln Thr Ala Ala Ala Val Leu Ala                                         
    450                 455                                             


<210>  2
<211>  456
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  2

Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu 
1               5                   10                  15      


Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn 
            20                  25                  30          


Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp 
        35                  40                  45              


Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys 
    50                  55                  60                  


Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg 
65                  70                  75                  80  


Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His 
                85                  90                  95      


Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu 
            100                 105                 110         


Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp 
        115                 120                 125             


Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro 
    130                 135                 140                 


Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr 
145                 150                 155                 160 


Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly 
                165                 170                 175     


Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr 
            180                 185                 190         


Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala 
        195                 200                 205             


Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala 
    210                 215                 220                 


Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro 
225                 230                 235                 240 


Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp 
                245                 250                 255     


Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly 
            260                 265                 270         


Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro 
        275                 280                 285             


Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val 
    290                 295                 300                 


Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr 
305                 310                 315                 320 


His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu 
                325                 330                 335     


Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys 
            340                 345                 350         


Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp 
        355                 360                 365             


His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu 
    370                 375                 380                 


Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly 
385                 390                 395                 400 


Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met 
                405                 410                 415     


Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp 
            420                 425                 430         


Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp 
        435                 440                 445             


Gln Thr Ala Ala Ala Val Leu Ala 
    450                 455     


<210>  3
<211>  1407
<212>  DNA
<213>  Pseudomonas aeruginosa


<220>
<221>  CDS
<222>  (1)..(1407)
<223>  amino transferase gi9951072

<400>  3
atg aac gca aga ctg cac gcc acg tcc ccc ctc ggc gac gcc gac ctg       48
Met Asn Ala Arg Leu His Ala Thr Ser Pro Leu Gly Asp Ala Asp Leu         
1               5                   10                  15              

gtc cgt gcc gac cag gcc cac tac atg cac ggc tac cac gtg ttc gac       96
Val Arg Ala Asp Gln Ala His Tyr Met His Gly Tyr His Val Phe Asp         
            20                  25                  30                  

gac cac cgc gtc aac ggc tcg ctg aac atc gcc gcc ggc gac ggc gcc      144
Asp His Arg Val Asn Gly Ser Leu Asn Ile Ala Ala Gly Asp Gly Ala         
        35                  40                  45                      

tat atc tac gac acc gcc ggc aac cgc tac ctc gac gcg gtg ggc ggc      192
Tyr Ile Tyr Asp Thr Ala Gly Asn Arg Tyr Leu Asp Ala Val Gly Gly         
    50                  55                  60                          

atg tgg tgc acc aac atc ggc ctg ggg cgc gag gaa atg gct cgc acc      240
Met Trp Cys Thr Asn Ile Gly Leu Gly Arg Glu Glu Met Ala Arg Thr         
65                  70                  75                  80          

gtg gcc gag cag acc cgc ctg ctg gcc tat tcc aat ccc ttc tgc gac      288
Val Ala Glu Gln Thr Arg Leu Leu Ala Tyr Ser Asn Pro Phe Cys Asp         
                85                  90                  95              

atg gcc aac ccg cgc gcc atc gaa ctc tgc cgc aag ctc gcc gag ctg      336
Met Ala Asn Pro Arg Ala Ile Glu Leu Cys Arg Lys Leu Ala Glu Leu         
            100                 105                 110                 

gcc ccc ggc gac ctc gac cac gtg ttc ctc acc acc ggc ggt tcc acc      384
Ala Pro Gly Asp Leu Asp His Val Phe Leu Thr Thr Gly Gly Ser Thr         
        115                 120                 125                     

gcc gtg gac acc gcg atc cgc ctc atg cac tac tac cag aac tgc cgc      432
Ala Val Asp Thr Ala Ile Arg Leu Met His Tyr Tyr Gln Asn Cys Arg         
    130                 135                 140                         

ggc aag cgc gcc aag aag cac gtc atc acg cgg atc aac gcc tac cac      480
Gly Lys Arg Ala Lys Lys His Val Ile Thr Arg Ile Asn Ala Tyr His         
145                 150                 155                 160         

ggc tcg acc ttc ctc ggc atg tcg ctg ggc ggc aag agc gcc gac cgg      528
Gly Ser Thr Phe Leu Gly Met Ser Leu Gly Gly Lys Ser Ala Asp Arg         
                165                 170                 175             

ccg gcc gag ttc gac ttc ctc gac gag cgc atc cac cac ctc gcc tgt      576
Pro Ala Glu Phe Asp Phe Leu Asp Glu Arg Ile His His Leu Ala Cys         
            180                 185                 190                 

ccc tat tac tac cgc gct ccg gaa ggg ctg ggc gaa gcc gag ttc ctc      624
Pro Tyr Tyr Tyr Arg Ala Pro Glu Gly Leu Gly Glu Ala Glu Phe Leu         
        195                 200                 205                     

gat ggc ctg gtg gac gag ttc gaa cgc aag atc ctc gaa ctg ggc gcc      672
Asp Gly Leu Val Asp Glu Phe Glu Arg Lys Ile Leu Glu Leu Gly Ala         
    210                 215                 220                         

gac cgg gtg ggg gcg ttc atc tcc gag ccg gtg ttc ggc tcc ggc ggc      720
Asp Arg Val Gly Ala Phe Ile Ser Glu Pro Val Phe Gly Ser Gly Gly         
225                 230                 235                 240         

gtg atc gtc ccg ccc gcg ggc tac cac agg cgg atg tgg gag ctg tgc      768
Val Ile Val Pro Pro Ala Gly Tyr His Arg Arg Met Trp Glu Leu Cys         
                245                 250                 255             

cag cgc tac gac gtg ctg tac atc tcc gac gaa gtg gtg acc tcc ttc      816
Gln Arg Tyr Asp Val Leu Tyr Ile Ser Asp Glu Val Val Thr Ser Phe         
            260                 265                 270                 

ggc cgc ctc ggc cac ttc ttc gcc agc cag gcg gtg ttc ggc gta cag      864
Gly Arg Leu Gly His Phe Phe Ala Ser Gln Ala Val Phe Gly Val Gln         
        275                 280                 285                     

ccg gac atc atc ctc acc gcc aag ggc ctc acc tcc ggc tac cag ccg      912
Pro Asp Ile Ile Leu Thr Ala Lys Gly Leu Thr Ser Gly Tyr Gln Pro         
    290                 295                 300                         

ctg ggc gcg tgc atc ttc tcc cgg cgc atc tgg gag gtg atc gcc gag      960
Leu Gly Ala Cys Ile Phe Ser Arg Arg Ile Trp Glu Val Ile Ala Glu         
305                 310                 315                 320         

ccg gac aag ggc cgc tgc ttc agc cat ggt ttc acc tac tcc ggc cac     1008
Pro Asp Lys Gly Arg Cys Phe Ser His Gly Phe Thr Tyr Ser Gly His         
                325                 330                 335             

ccg gtg gcc tgc gcg gcg gcg ctg aag aac atc gag atc atc gag cgc     1056
Pro Val Ala Cys Ala Ala Ala Leu Lys Asn Ile Glu Ile Ile Glu Arg         
            340                 345                 350                 

gag ggc ttg ctc gcc cac gcc gac gag gtc ggc cgc tac ttc gag gag     1104
Glu Gly Leu Leu Ala His Ala Asp Glu Val Gly Arg Tyr Phe Glu Glu         
        355                 360                 365                     

cgc ctg caa agc ctc cgc gac ctg ccc atc gtc ggc gac gtg cgc ggg     1152
Arg Leu Gln Ser Leu Arg Asp Leu Pro Ile Val Gly Asp Val Arg Gly         
    370                 375                 380                         

atg cgc ttc atg gcc tgt gtc gag ttc gtc gcc gac aag gcg agc aag     1200
Met Arg Phe Met Ala Cys Val Glu Phe Val Ala Asp Lys Ala Ser Lys         
385                 390                 395                 400         

gcg ctg ttt ccg gaa agc ctg aac atc ggc gag tgg gtc cac ctg cgg     1248
Ala Leu Phe Pro Glu Ser Leu Asn Ile Gly Glu Trp Val His Leu Arg         
                405                 410                 415             

gcg cag aag cgc ggc ctg ctg gtt cgt ccg atc gtc cac ctg aac gtg     1296
Ala Gln Lys Arg Gly Leu Leu Val Arg Pro Ile Val His Leu Asn Val         
            420                 425                 430                 

atg tcg ccg ccg ctg atc ctc acc cgc gaa cag gtc gat acc gtg gtc     1344
Met Ser Pro Pro Leu Ile Leu Thr Arg Glu Gln Val Asp Thr Val Val         
        435                 440                 445                     

cgg gtg ctg cgc gag agc atc gag gaa acc gtg gag gat ctt gtc cgc     1392
Arg Val Leu Arg Glu Ser Ile Glu Glu Thr Val Glu Asp Leu Val Arg         
    450                 455                 460                         

gcc ggt cac cgg taa                                                 1407
Ala Gly His Arg                                                         
465                                                                     


<210>  4
<211>  468
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  4

Met Asn Ala Arg Leu His Ala Thr Ser Pro Leu Gly Asp Ala Asp Leu 
1               5                   10                  15      


Val Arg Ala Asp Gln Ala His Tyr Met His Gly Tyr His Val Phe Asp 
            20                  25                  30          


Asp His Arg Val Asn Gly Ser Leu Asn Ile Ala Ala Gly Asp Gly Ala 
        35                  40                  45              


Tyr Ile Tyr Asp Thr Ala Gly Asn Arg Tyr Leu Asp Ala Val Gly Gly 
    50                  55                  60                  


Met Trp Cys Thr Asn Ile Gly Leu Gly Arg Glu Glu Met Ala Arg Thr 
65                  70                  75                  80  


Val Ala Glu Gln Thr Arg Leu Leu Ala Tyr Ser Asn Pro Phe Cys Asp 
                85                  90                  95      


Met Ala Asn Pro Arg Ala Ile Glu Leu Cys Arg Lys Leu Ala Glu Leu 
            100                 105                 110         


Ala Pro Gly Asp Leu Asp His Val Phe Leu Thr Thr Gly Gly Ser Thr 
        115                 120                 125             


Ala Val Asp Thr Ala Ile Arg Leu Met His Tyr Tyr Gln Asn Cys Arg 
    130                 135                 140                 


Gly Lys Arg Ala Lys Lys His Val Ile Thr Arg Ile Asn Ala Tyr His 
145                 150                 155                 160 


Gly Ser Thr Phe Leu Gly Met Ser Leu Gly Gly Lys Ser Ala Asp Arg 
                165                 170                 175     


Pro Ala Glu Phe Asp Phe Leu Asp Glu Arg Ile His His Leu Ala Cys 
            180                 185                 190         


Pro Tyr Tyr Tyr Arg Ala Pro Glu Gly Leu Gly Glu Ala Glu Phe Leu 
        195                 200                 205             


Asp Gly Leu Val Asp Glu Phe Glu Arg Lys Ile Leu Glu Leu Gly Ala 
    210                 215                 220                 


Asp Arg Val Gly Ala Phe Ile Ser Glu Pro Val Phe Gly Ser Gly Gly 
225                 230                 235                 240 


Val Ile Val Pro Pro Ala Gly Tyr His Arg Arg Met Trp Glu Leu Cys 
                245                 250                 255     


Gln Arg Tyr Asp Val Leu Tyr Ile Ser Asp Glu Val Val Thr Ser Phe 
            260                 265                 270         


Gly Arg Leu Gly His Phe Phe Ala Ser Gln Ala Val Phe Gly Val Gln 
        275                 280                 285             


Pro Asp Ile Ile Leu Thr Ala Lys Gly Leu Thr Ser Gly Tyr Gln Pro 
    290                 295                 300                 


Leu Gly Ala Cys Ile Phe Ser Arg Arg Ile Trp Glu Val Ile Ala Glu 
305                 310                 315                 320 


Pro Asp Lys Gly Arg Cys Phe Ser His Gly Phe Thr Tyr Ser Gly His 
                325                 330                 335     


Pro Val Ala Cys Ala Ala Ala Leu Lys Asn Ile Glu Ile Ile Glu Arg 
            340                 345                 350         


Glu Gly Leu Leu Ala His Ala Asp Glu Val Gly Arg Tyr Phe Glu Glu 
        355                 360                 365             


Arg Leu Gln Ser Leu Arg Asp Leu Pro Ile Val Gly Asp Val Arg Gly 
    370                 375                 380                 


Met Arg Phe Met Ala Cys Val Glu Phe Val Ala Asp Lys Ala Ser Lys 
385                 390                 395                 400 


Ala Leu Phe Pro Glu Ser Leu Asn Ile Gly Glu Trp Val His Leu Arg 
                405                 410                 415     


Ala Gln Lys Arg Gly Leu Leu Val Arg Pro Ile Val His Leu Asn Val 
            420                 425                 430         


Met Ser Pro Pro Leu Ile Leu Thr Arg Glu Gln Val Asp Thr Val Val 
        435                 440                 445             


Arg Val Leu Arg Glu Ser Ile Glu Glu Thr Val Glu Asp Leu Val Arg 
    450                 455                 460                 


Ala Gly His Arg 
465             


<210>  5
<211>  1362
<212>  DNA
<213>  Vibrio fluvialis


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  omega-aminotransferase JS17

<400>  5
atg aac aaa ccg caa agc tgg gaa gcc cgg gcc gag acc tat tcg ctc       48
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu         
1               5                   10                  15              

tat ggt ttc acc gac atg cct tcg ctg cat cag cgc ggc acg gtc gtc       96
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val         
            20                  25                  30                  

gtg acc cat ggc gag gga ccc tat atc gtc gat gtg aat ggc cgg cgt      144
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg         
        35                  40                  45                      

tat ctg gac gcc aac tcg ggc ctg tgg aac atg gtc gcg ggc ttt gac      192
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp         
    50                  55                  60                          

cac aag ggg ctg atc gac gcc gcc aag gcc caa tac gag cgt ttt ccc      240
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro         
65                  70                  75                  80          

ggt tat cac gcc ttt ttc ggc cgc atg tcc gat cag acg gta atg ctg      288
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu         
                85                  90                  95              

tcg gaa aag ctg gtc gag gtg tcg ccc ttt gat tcg ggc cgg gtg ttc      336
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe         
            100                 105                 110                 

tat aca aac tcg ggg tcc gag gcg aat gac acc atg gtc aag atg cta      384
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu         
        115                 120                 125                     

tgg ttc ctg cat gca gcc gag ggc aaa ccg caa aag cgc aag atc ctg      432
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu         
    130                 135                 140                         

acc cgc tgg aac gcc tat cac ggc gtg acc gcc gtt tcg gcc agc atg      480
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met         
145                 150                 155                 160         

acc ggc aag ccc tat aat tcg gtc ttt ggc ctg ccg ctg ccg ggc ttt      528
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe         
                165                 170                 175             

gtg cat ctg acc tgc ccg cat tac tgg cgc tat ggc gaa gag ggc gaa      576
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu         
            180                 185                 190                 

acc gaa gag cag ttc gtc gcc cgc ctc gcc cgc gag ctg gag gaa acg      624
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr         
        195                 200                 205                     

atc cag cgc gag ggc gcc gac acc atc gcc ggt ttc ttt gcc gaa ccg      672
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro         
    210                 215                 220                         

gtg atg ggc gcg ggc ggc gtg att ccc ccg gcc aag ggc tat ttc cag      720
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln         
225                 230                 235                 240         

gcg atc ctg cca atc ctg cgc aaa tat gac atc ccg gtc atc tcg gac      768
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp         
                245                 250                 255             

gag gtg atc tgc ggt ttc gga cgc acc ggt aac acc tgg ggc tgc gtg      816
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val         
            260                 265                 270                 

acc tat gac ttt aca ccc gat gca atc atc tcg tcc aag aat ctt aca      864
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr         
        275                 280                 285                     

gcg ggc ttt ttc ccc atg ggg gcg gtg atc ctt ggc ccg gaa ctt tcc      912
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser         
    290                 295                 300                         

aaa cgg ctg gaa acc gca atc gag gcg atc gag gaa ttc ccc cat ggc      960
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly         
305                 310                 315                 320         

ttt acc gcc tcg ggc cat ccg gtc ggc tgt gct att gcg ctg aaa gca     1008
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala         
                325                 330                 335             

atc gac gtg gtg atg aat gaa ggg ctg gct gag aac gtc cgc cgc ctt     1056
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu         
            340                 345                 350                 

gcc ccc cgt ttc gag gaa agg ctg aaa cat atc gcc gag cgc ccg aac     1104
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn         
        355                 360                 365                     

atc ggt gaa tat cgc ggc atc ggc ttc atg tgg gcg ctg gag gct gtc     1152
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val         
    370                 375                 380                         

aag gac aag gca agc aag acg ccg ttc gac ggc aac ctg tcg gtc agc     1200
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser         
385                 390                 395                 400         

gag cgt atc gcc aat acc tgc acc gat ctg ggg ctg att tgc cgg ccg     1248
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro         
                405                 410                 415             

ctt ggt cag tcc gtc gtc ctt tgt ccg ccc ttt atc ctg acc gag gcg     1296
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala         
            420                 425                 430                 

cag atg gat gag atg ttc gat aaa ctc gaa aaa gcc ctt gat aag gtc     1344
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val         
        435                 440                 445                     

ttt gcc gag gtt gcc tga                                             1362
Phe Ala Glu Val Ala                                                     
    450                                                                 


<210>  6
<211>  453
<212>  PRT
<213>  Vibrio fluvialis

<400>  6

Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 


Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  7
<211>  1362
<212>  DNA
<213>  Artificial

<220>
<223>  codon optimised sequence of omega-aminotransferase JS17

<400>  7
atgaacaagc cgcagtcctg ggaagcgcgt gctgaaactt actctctgta cggcttcact     60

gacatgccgt ctctgcacca gcgtggtact gttgttgtta ctcacggtga aggtccgtac    120

atcgttgacg ttaacggtcg tcgttacctg gatgctaact ccggtctgtg gaacatggtt    180

gctggcttcg accacaaagg tctgatcgac gcagctaaag cgcagtacga acgtttccca    240

ggttaccacg cattcttcgg tcgtatgtct gaccagactg taatgctgtc tgagaagctg    300

gttgaagttt ctccgttcga ctccggtcgc gtattctaca ccaactccgg ttctgaagct    360

aacgacacca tggttaaaat gctgtggttc ctgcacgctg cagaaggtaa gccgcagaag    420

cgtaagatcc tgactcgctg gaacgcttac cacggcgtaa ctgctgtttc tgcttctatg    480

accggtaagc cgtacaactc cgtattcggt ctgccgctgc cgggcttcgt tcacctgact    540

tgcccgcact actggcgtta cggtgaagaa ggtgaaactg aagagcagtt cgttgctcgt    600

ctggcacgtg aactggaaga aaccattcag cgtgaaggtg ctgacaccat cgctggcttc    660

ttcgctgaac cggtaatggg cgcaggtggt gttatcccgc cagcaaaagg ttacttccag    720

gctatcctgc cgattctgcg taaatacgac atcccggtta tctctgacga agttatctgc    780

ggtttcggtc gtactggtaa cacctggggc tgcgtaactt acgacttcac tccggatgct    840

atcatctctt ccaaaaacct gactgcaggc ttcttcccga tgggtgctgt tatcctgggt    900

ccggaactgt ctaaacgtct ggaaactgct atcgaagcaa tcgaagagtt cccgcacggc    960

ttcactgctt ctggtcaccc ggttggttgc gcaatcgcgc tgaaagcaat cgacgttgta   1020

atgaacgaag gtctggcaga aaacgttcgt cgtctggcac cgcgcttcga agagcgtctg   1080

aaacacatcg ctgaacgtcc gaacatcggt gaataccgcg gtatcggctt catgtgggcg   1140

ctggaagcgg ttaaagacaa agcgtctaaa actccgttcg acggtaacct gtctgtttct   1200

gaacgtatcg ctaacacctg tactgacctg ggtctgatct gccgtccgct gggtcagtct   1260

gttgttctgt gcccgccgtt catcctgact gaagcgcaga tggacgaaat gttcgacaaa   1320

ctggaaaaag cgctggataa agttttcgct gaagttgcgt aa                      1362


<210>  8
<211>  1644
<212>  DNA
<213>  Lactococcus lactis


<220>
<221>  CDS
<222>  (1)..(1644)
<223>  decarboxylase KdcA

<400>  8
atg tat aca gta gga gat tac ctg tta gac cga tta cac gag ttg gga       48
Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly         
1               5                   10                  15              

att gaa gaa att ttt gga gtt cct ggt gac tat aac tta caa ttt tta       96
Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu         
            20                  25                  30                  

gat caa att att tca cgc gaa gat atg aaa tgg att gga aat gct aat      144
Asp Gln Ile Ile Ser Arg Glu Asp Met Lys Trp Ile Gly Asn Ala Asn         
        35                  40                  45                      

gaa tta aat gct tct tat atg gct gat ggt tat gct cgt act aaa aaa      192
Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys         
    50                  55                  60                          

gct gcc gca ttt ctc acc aca ttt gga gtc ggc gaa ttg agt gcg atc      240
Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile         
65                  70                  75                  80          

aat gga ctg gca gga agt tat gcc gaa aat tta cca gta gta gaa att      288
Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile         
                85                  90                  95              

gtt ggt tca cca act tca aaa gta caa aat gac gga aaa ttt gtc cat      336
Val Gly Ser Pro Thr Ser Lys Val Gln Asn Asp Gly Lys Phe Val His         
            100                 105                 110                 

cat aca cta gca gat ggt gat ttt aaa cac ttt atg aag atg cat gaa      384
His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu         
        115                 120                 125                     

cct gtt aca gca gcg cgg act tta ctg aca gca gaa aat gcc aca tat      432
Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Tyr         
    130                 135                 140                         

gaa att gac cga gta ctt tct caa tta cta aaa gaa aga aaa cca gtc      480
Glu Ile Asp Arg Val Leu Ser Gln Leu Leu Lys Glu Arg Lys Pro Val         
145                 150                 155                 160         

tat att aac tta cca gtc gat gtt gct gca gca aaa gca gag aag cct      528
Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro         
                165                 170                 175             

gca tta tct tta gaa aaa gaa agc tct aca aca aat aca act gaa caa      576
Ala Leu Ser Leu Glu Lys Glu Ser Ser Thr Thr Asn Thr Thr Glu Gln         
            180                 185                 190                 

gtg att ttg agt aag att gaa gaa agt ttg aaa aat gcc caa aaa cca      624
Val Ile Leu Ser Lys Ile Glu Glu Ser Leu Lys Asn Ala Gln Lys Pro         
        195                 200                 205                     

gta gtg att gca gga cac gaa gta att agt ttt ggt tta gaa aaa acg      672
Val Val Ile Ala Gly His Glu Val Ile Ser Phe Gly Leu Glu Lys Thr         
    210                 215                 220                         

gta act cag ttt gtt tca gaa aca aaa cta ccg att acg aca cta aat      720
Val Thr Gln Phe Val Ser Glu Thr Lys Leu Pro Ile Thr Thr Leu Asn         
225                 230                 235                 240         

ttt ggt aaa agt gct gtt gat gaa tct ttg ccc tca ttt tta gga ata      768
Phe Gly Lys Ser Ala Val Asp Glu Ser Leu Pro Ser Phe Leu Gly Ile         
                245                 250                 255             

tat aac ggg aaa ctt tca gaa atc agt ctt aaa aat ttt gtg gag tcc      816
Tyr Asn Gly Lys Leu Ser Glu Ile Ser Leu Lys Asn Phe Val Glu Ser         
            260                 265                 270                 

gca gac ttt atc cta atg ctt gga gtg aag ctt acg gac tcc tca aca      864
Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr         
        275                 280                 285                     

ggt gca ttc aca cat cat tta gat gaa aat aaa atg att tca cta aac      912
Gly Ala Phe Thr His His Leu Asp Glu Asn Lys Met Ile Ser Leu Asn         
    290                 295                 300                         

ata gat gaa gga ata att ttc aat aaa gtg gta gaa gat ttt gat ttt      960
Ile Asp Glu Gly Ile Ile Phe Asn Lys Val Val Glu Asp Phe Asp Phe         
305                 310                 315                 320         

aga gca gtg gtt tct tct tta tca gaa tta aaa gga ata gaa tat gaa     1008
Arg Ala Val Val Ser Ser Leu Ser Glu Leu Lys Gly Ile Glu Tyr Glu         
                325                 330                 335             

gga caa tat att gat aag caa tat gaa gaa ttt att cca tca agt gct     1056
Gly Gln Tyr Ile Asp Lys Gln Tyr Glu Glu Phe Ile Pro Ser Ser Ala         
            340                 345                 350                 

ccc tta tca caa gac cgt cta tgg cag gca gtt gaa agt ttg act caa     1104
Pro Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Ser Leu Thr Gln         
        355                 360                 365                     

agc aat gaa aca atc gtt gct gaa caa gga acc tca ttt ttt gga gct     1152
Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala         
    370                 375                 380                         

tca aca att ttc tta aaa tca aat agt cgt ttt att gga caa cct tta     1200
Ser Thr Ile Phe Leu Lys Ser Asn Ser Arg Phe Ile Gly Gln Pro Leu         
385                 390                 395                 400         

tgg ggt tct att gga tat act ttt cca gcg gct tta gga agc caa att     1248
Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile         
                405                 410                 415             

gcg gat aaa gag agc aga cac ctt tta ttt att ggt gat ggt tca ctt     1296
Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu         
            420                 425                 430                 

caa ctt acc gta caa gaa tta gga cta tca atc aga gaa aaa ctc aat     1344
Gln Leu Thr Val Gln Glu Leu Gly Leu Ser Ile Arg Glu Lys Leu Asn         
        435                 440                 445                     

cca att tgt ttt atc ata aat aat gat ggt tat aca gtt gaa aga gaa     1392
Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu         
    450                 455                 460                         

atc cac gga cct act caa agt tat aac gac att cca atg tgg aat tac     1440
Ile His Gly Pro Thr Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr         
465                 470                 475                 480         

tcg aaa tta cca gaa aca ttt gga gca aca gaa gat cgt gta gta tca     1488
Ser Lys Leu Pro Glu Thr Phe Gly Ala Thr Glu Asp Arg Val Val Ser         
                485                 490                 495             

aaa att gtt aga aca gag aat gaa ttt gtg tct gtc atg aaa gaa gcc     1536
Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala         
            500                 505                 510                 

caa gca gat gtc aat aga atg tat tgg ata gaa cta gtt ttg gaa aaa     1584
Gln Ala Asp Val Asn Arg Met Tyr Trp Ile Glu Leu Val Leu Glu Lys         
        515                 520                 525                     

gaa gat gcg cca aaa tta ctg aaa aaa atg ggt aaa tta ttt gct gag     1632
Glu Asp Ala Pro Lys Leu Leu Lys Lys Met Gly Lys Leu Phe Ala Glu         
    530                 535                 540                         

caa aat aaa tag                                                     1644
Gln Asn Lys                                                             
545                                                                     


<210>  9
<211>  547
<212>  PRT
<213>  Lactococcus lactis

<400>  9

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser Arg Glu Asp Met Lys Trp Ile Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Asp Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Tyr 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Gln Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ala Leu Ser Leu Glu Lys Glu Ser Ser Thr Thr Asn Thr Thr Glu Gln 
            180                 185                 190         


Val Ile Leu Ser Lys Ile Glu Glu Ser Leu Lys Asn Ala Gln Lys Pro 
        195                 200                 205             


Val Val Ile Ala Gly His Glu Val Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Thr Gln Phe Val Ser Glu Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ala Val Asp Glu Ser Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Lys Leu Ser Glu Ile Ser Leu Lys Asn Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asp Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Ile Ile Phe Asn Lys Val Val Glu Asp Phe Asp Phe 
305                 310                 315                 320 


Arg Ala Val Val Ser Ser Leu Ser Glu Leu Lys Gly Ile Glu Tyr Glu 
                325                 330                 335     


Gly Gln Tyr Ile Asp Lys Gln Tyr Glu Glu Phe Ile Pro Ser Ser Ala 
            340                 345                 350         


Pro Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Ser Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Thr Ile Phe Leu Lys Ser Asn Ser Arg Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ser Ile Arg Glu Lys Leu Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Thr Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Thr Phe Gly Ala Thr Glu Asp Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Val Asn Arg Met Tyr Trp Ile Glu Leu Val Leu Glu Lys 
        515                 520                 525             


Glu Asp Ala Pro Lys Leu Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys 
545         


<210>  10
<211>  1647
<212>  DNA
<213>  Lactococcus lactis


<220>
<221>  CDS
<222>  (1)..(1647)
<223>  decarboxylase KivD

<400>  10
atg tat aca gta gga gat tac cta tta gac cga tta cac gag tta gga       48
Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly         
1               5                   10                  15              

att gaa gaa att ttt gga gtc cct gga gac tat aac tta caa ttt tta       96
Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu         
            20                  25                  30                  

gat caa att att tcc cac aag gat atg aaa tgg gtc gga aat gct aat      144
Asp Gln Ile Ile Ser His Lys Asp Met Lys Trp Val Gly Asn Ala Asn         
        35                  40                  45                      

gaa tta aat gct tca tat atg gct gat ggc tat gct cgt act aaa aaa      192
Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys         
    50                  55                  60                          

gct gcc gca ttt ctt aca acc ttt gga gta ggt gaa ttg agt gca gtt      240
Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val         
65                  70                  75                  80          

aat gga tta gca gga agt tac gcc gaa aat tta cca gta gta gaa ata      288
Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile         
                85                  90                  95              

gtg gga tca cct aca tca aaa gtt caa aat gaa gga aaa ttt gtt cat      336
Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His         
            100                 105                 110                 

cat acg ctg gct gac ggt gat ttt aaa cac ttt atg aaa atg cac gaa      384
His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu         
        115                 120                 125                     

cct gtt aca gca gct cga act tta ctg aca gca gaa aat gca acc gtt      432
Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val         
    130                 135                 140                         

gaa att gac cga gta ctt tct gca cta tta aaa gaa aga aaa cct gtc      480
Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val         
145                 150                 155                 160         

tat atc aac tta cca gtt gat gtt gct gct gca aaa gca gag aaa ccc      528
Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro         
                165                 170                 175             

tca ctc cct ttg aaa aag gaa aac tca act tca aat aca agt gac caa      576
Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln         
            180                 185                 190                 

gaa att ttg aac aaa att caa gaa agc ttg aaa aat gcc aaa aaa cca      624
Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro         
        195                 200                 205                     

atc gtg att aca gga cat gaa ata att agt ttt ggc tta gaa aaa aca      672
Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr         
    210                 215                 220                         

gtc act caa ttt att tca aag aca aaa cta cct att acg aca tta aac      720
Val Thr Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn         
225                 230                 235                 240         

ttt ggt aaa agt tca gtt gat gaa gcc ctc cct tca ttt tta gga atc      768
Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile         
                245                 250                 255             

tat aat ggt aca ctc tca gag cct aat ctt aaa gaa ttc gtg gaa tca      816
Tyr Asn Gly Thr Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser         
            260                 265                 270                 

gcc gac ttc atc ttg atg ctt gga gtt aaa ctc aca gac tct tca aca      864
Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr         
        275                 280                 285                     

gga gcc ttc act cat cat tta aat gaa aat aaa atg att tca ctg aat      912
Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn         
    290                 295                 300                         

ata gat gaa gga aaa ata ttt aac gaa aga atc caa aat ttt gat ttt      960
Ile Asp Glu Gly Lys Ile Phe Asn Glu Arg Ile Gln Asn Phe Asp Phe         
305                 310                 315                 320         

gaa tcc ctc atc tcc tct ctc tta gac cta agc gaa ata gaa tac aaa     1008
Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys         
                325                 330                 335             

gga aaa tat atc gat aaa aag caa gaa gac ttt gtt cca tca aat gcg     1056
Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala         
            340                 345                 350                 

ctt tta tca caa gac cgc cta tgg caa gca gtt gaa aac cta act caa     1104
Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln         
        355                 360                 365                     

agc aat gaa aca atc gtt gct gaa caa ggg aca tca ttc ttt ggc gct     1152
Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala         
    370                 375                 380                         

tca tca att ttc tta aaa tca aag agt cat ttt att ggt caa ccc tta     1200
Ser Ser Ile Phe Leu Lys Ser Lys Ser His Phe Ile Gly Gln Pro Leu         
385                 390                 395                 400         

tgg gga tca att gga tat aca ttc cca gca gca tta gga agc caa att     1248
Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile         
                405                 410                 415             

gca gat aaa gaa agc aga cac ctt tta ttt att ggt gat ggt tca ctt     1296
Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu         
            420                 425                 430                 

caa ctt aca gtg caa gaa tta gga tta gca atc aga gaa aaa att aat     1344
Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn         
        435                 440                 445                     

cca att tgc ttt att atc aat aat gat ggt tat aca gtc gaa aga gaa     1392
Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu         
    450                 455                 460                         

att cat gga cca aat caa agc tac aat gat att cca atg tgg aat tac     1440
Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr         
465                 470                 475                 480         

tca aaa tta cca gaa tcg ttt gga gca aca gaa gat cga gta gtc tca     1488
Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Asp Arg Val Val Ser         
                485                 490                 495             

aaa atc gtt aga act gaa aat gaa ttt gtg tct gtc atg aaa gaa gct     1536
Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala         
            500                 505                 510                 

caa gca gat cca aat aga atg tac tgg att gag tta att ttg gca aaa     1584
Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys         
        515                 520                 525                     

gaa ggt gca cca aaa gta ctg aaa aaa atg ggc aaa cta ttt gct gaa     1632
Glu Gly Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu         
    530                 535                 540                         

caa aat aaa tca taa                                                 1647
Gln Asn Lys Ser                                                         
545                                                                     


<210>  11
<211>  548
<212>  PRT
<213>  Lactococcus lactis

<400>  11

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser His Lys Asp Met Lys Trp Val Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln 
            180                 185                 190         


Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro 
        195                 200                 205             


Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Thr Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Thr Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Lys Ile Phe Asn Glu Arg Ile Gln Asn Phe Asp Phe 
305                 310                 315                 320 


Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys 
                325                 330                 335     


Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala 
            340                 345                 350         


Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Ser Ile Phe Leu Lys Ser Lys Ser His Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Asp Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys 
        515                 520                 525             


Glu Gly Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys Ser 
545             


<210>  12
<211>  1263
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1263)
<223>  decarboxylase LysA

<400>  12
atg cca cat tca ctg ttc agc acc gat acc gat ctc acc gcc gaa aat       48
Met Pro His Ser Leu Phe Ser Thr Asp Thr Asp Leu Thr Ala Glu Asn         
1               5                   10                  15              

ctg ctg cgt ttg ccc gct gaa ttt ggc tgc ccg gtg tgg gtc tac gat       96
Leu Leu Arg Leu Pro Ala Glu Phe Gly Cys Pro Val Trp Val Tyr Asp         
            20                  25                  30                  

gcg caa att att cgt cgg cag att gca gcg ctg aaa cag ttt gat gtg      144
Ala Gln Ile Ile Arg Arg Gln Ile Ala Ala Leu Lys Gln Phe Asp Val         
        35                  40                  45                      

gtg cgc ttt gca cag aaa gcc tgt tcc aat att cat att ttg cgc tta      192
Val Arg Phe Ala Gln Lys Ala Cys Ser Asn Ile His Ile Leu Arg Leu         
    50                  55                  60                          

atg cgt gag cag ggc gtg aaa gtg gat tcc gtc tcg tta ggc gaa ata      240
Met Arg Glu Gln Gly Val Lys Val Asp Ser Val Ser Leu Gly Glu Ile         
65                  70                  75                  80          

gag cgt gcg ttg gcg gcg ggt tac aat ccg caa acg cac ccc gat gat      288
Glu Arg Ala Leu Ala Ala Gly Tyr Asn Pro Gln Thr His Pro Asp Asp         
                85                  90                  95              

att gtt ttt acg gca gat gtt atc gat cag gcg acg ctt gaa cgc gtc      336
Ile Val Phe Thr Ala Asp Val Ile Asp Gln Ala Thr Leu Glu Arg Val         
            100                 105                 110                 

agt gaa ttg caa att ccg gtg aat gcg ggt tct gtt gat atg ctc gac      384
Ser Glu Leu Gln Ile Pro Val Asn Ala Gly Ser Val Asp Met Leu Asp         
        115                 120                 125                     

caa ctg ggc cag gtt tcg cca ggg cat cgg gta tgg ctg cgc gtt aat      432
Gln Leu Gly Gln Val Ser Pro Gly His Arg Val Trp Leu Arg Val Asn         
    130                 135                 140                         

ccg ggg ttt ggt cac gga cat agc caa aaa acc aat acc ggt ggc gaa      480
Pro Gly Phe Gly His Gly His Ser Gln Lys Thr Asn Thr Gly Gly Glu         
145                 150                 155                 160         

aac agc aag cac ggt atc tgg tac acc gat ctg ccc gcc gca ctg gac      528
Asn Ser Lys His Gly Ile Trp Tyr Thr Asp Leu Pro Ala Ala Leu Asp         
                165                 170                 175             

gtg ata caa cgt cat cat ctg cag ctg gtc ggc att cac atg cac att      576
Val Ile Gln Arg His His Leu Gln Leu Val Gly Ile His Met His Ile         
            180                 185                 190                 

ggt tct ggc gtt gat tat gcc cat ctg gaa cag gtg tgt ggt gct atg      624
Gly Ser Gly Val Asp Tyr Ala His Leu Glu Gln Val Cys Gly Ala Met         
        195                 200                 205                     

gtg cgt cag gtc atc gaa ttc ggt cag gat tta cag gct att tct gcg      672
Val Arg Gln Val Ile Glu Phe Gly Gln Asp Leu Gln Ala Ile Ser Ala         
    210                 215                 220                         

ggc ggt ggg ctt tct gtt cct tat caa cag ggt gaa gag gcg gtt gat      720
Gly Gly Gly Leu Ser Val Pro Tyr Gln Gln Gly Glu Glu Ala Val Asp         
225                 230                 235                 240         

acc gaa cat tat tat ggt ctg tgg aat gcc gcg cgt gag caa atc gcc      768
Thr Glu His Tyr Tyr Gly Leu Trp Asn Ala Ala Arg Glu Gln Ile Ala         
                245                 250                 255             

cgc cat ttg ggc cac cct gtg aaa ctg gaa att gaa ccg ggt cgc ttc      816
Arg His Leu Gly His Pro Val Lys Leu Glu Ile Glu Pro Gly Arg Phe         
            260                 265                 270                 

ctg gta gcg cag tct ggc gta tta att act cag gtg cgg agc gtc aaa      864
Leu Val Ala Gln Ser Gly Val Leu Ile Thr Gln Val Arg Ser Val Lys         
        275                 280                 285                     

caa atg ggg agc cgc cac ttt gtg ctg gtt gat gcc ggg ttc aac gat      912
Gln Met Gly Ser Arg His Phe Val Leu Val Asp Ala Gly Phe Asn Asp         
    290                 295                 300                         

ctg atg cgc ccg gca atg tac ggt agt tac cac cat atc agt gcc ctg      960
Leu Met Arg Pro Ala Met Tyr Gly Ser Tyr His His Ile Ser Ala Leu         
305                 310                 315                 320         

gca gct gat ggt cgt tct ctg gaa cac gcg cca acg gtg gaa acc gtc     1008
Ala Ala Asp Gly Arg Ser Leu Glu His Ala Pro Thr Val Glu Thr Val         
                325                 330                 335             

gtc gcc gga ccg tta tgt gaa tcg ggc gat gtc ttt acc cag cag gaa     1056
Val Ala Gly Pro Leu Cys Glu Ser Gly Asp Val Phe Thr Gln Gln Glu         
            340                 345                 350                 

ggg gga aat gtt gaa acc cgc gcc ttg ccg gaa gtg aag gca ggt gat     1104
Gly Gly Asn Val Glu Thr Arg Ala Leu Pro Glu Val Lys Ala Gly Asp         
        355                 360                 365                     

tat ctg gta ctg cat gat aca ggg gca tat ggc gca tca atg tca tcc     1152
Tyr Leu Val Leu His Asp Thr Gly Ala Tyr Gly Ala Ser Met Ser Ser         
    370                 375                 380                         

aac tac aat agc cgt ccg ctg tta cca gaa gtt ctg ttt gat aat ggt     1200
Asn Tyr Asn Ser Arg Pro Leu Leu Pro Glu Val Leu Phe Asp Asn Gly         
385                 390                 395                 400         

cag gcg cgg ttg att cgc cgt cgc cag acc atc gaa gaa tta ctg gcg     1248
Gln Ala Arg Leu Ile Arg Arg Arg Gln Thr Ile Glu Glu Leu Leu Ala         
                405                 410                 415             

ctg gaa ttg ctt taa                                                 1263
Leu Glu Leu Leu                                                         
            420                                                         


<210>  13
<211>  420
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Pro His Ser Leu Phe Ser Thr Asp Thr Asp Leu Thr Ala Glu Asn 
1               5                   10                  15      


Leu Leu Arg Leu Pro Ala Glu Phe Gly Cys Pro Val Trp Val Tyr Asp 
            20                  25                  30          


Ala Gln Ile Ile Arg Arg Gln Ile Ala Ala Leu Lys Gln Phe Asp Val 
        35                  40                  45              


Val Arg Phe Ala Gln Lys Ala Cys Ser Asn Ile His Ile Leu Arg Leu 
    50                  55                  60                  


Met Arg Glu Gln Gly Val Lys Val Asp Ser Val Ser Leu Gly Glu Ile 
65                  70                  75                  80  


Glu Arg Ala Leu Ala Ala Gly Tyr Asn Pro Gln Thr His Pro Asp Asp 
                85                  90                  95      


Ile Val Phe Thr Ala Asp Val Ile Asp Gln Ala Thr Leu Glu Arg Val 
            100                 105                 110         


Ser Glu Leu Gln Ile Pro Val Asn Ala Gly Ser Val Asp Met Leu Asp 
        115                 120                 125             


Gln Leu Gly Gln Val Ser Pro Gly His Arg Val Trp Leu Arg Val Asn 
    130                 135                 140                 


Pro Gly Phe Gly His Gly His Ser Gln Lys Thr Asn Thr Gly Gly Glu 
145                 150                 155                 160 


Asn Ser Lys His Gly Ile Trp Tyr Thr Asp Leu Pro Ala Ala Leu Asp 
                165                 170                 175     


Val Ile Gln Arg His His Leu Gln Leu Val Gly Ile His Met His Ile 
            180                 185                 190         


Gly Ser Gly Val Asp Tyr Ala His Leu Glu Gln Val Cys Gly Ala Met 
        195                 200                 205             


Val Arg Gln Val Ile Glu Phe Gly Gln Asp Leu Gln Ala Ile Ser Ala 
    210                 215                 220                 


Gly Gly Gly Leu Ser Val Pro Tyr Gln Gln Gly Glu Glu Ala Val Asp 
225                 230                 235                 240 


Thr Glu His Tyr Tyr Gly Leu Trp Asn Ala Ala Arg Glu Gln Ile Ala 
                245                 250                 255     


Arg His Leu Gly His Pro Val Lys Leu Glu Ile Glu Pro Gly Arg Phe 
            260                 265                 270         


Leu Val Ala Gln Ser Gly Val Leu Ile Thr Gln Val Arg Ser Val Lys 
        275                 280                 285             


Gln Met Gly Ser Arg His Phe Val Leu Val Asp Ala Gly Phe Asn Asp 
    290                 295                 300                 


Leu Met Arg Pro Ala Met Tyr Gly Ser Tyr His His Ile Ser Ala Leu 
305                 310                 315                 320 


Ala Ala Asp Gly Arg Ser Leu Glu His Ala Pro Thr Val Glu Thr Val 
                325                 330                 335     


Val Ala Gly Pro Leu Cys Glu Ser Gly Asp Val Phe Thr Gln Gln Glu 
            340                 345                 350         


Gly Gly Asn Val Glu Thr Arg Ala Leu Pro Glu Val Lys Ala Gly Asp 
        355                 360                 365             


Tyr Leu Val Leu His Asp Thr Gly Ala Tyr Gly Ala Ser Met Ser Ser 
    370                 375                 380                 


Asn Tyr Asn Ser Arg Pro Leu Leu Pro Glu Val Leu Phe Asp Asn Gly 
385                 390                 395                 400 


Gln Ala Arg Leu Ile Arg Arg Arg Gln Thr Ile Glu Glu Leu Leu Ala 
                405                 410                 415     


Leu Glu Leu Leu 
            420 


<210>  14
<211>  1362
<212>  DNA
<213>  Paracoccus denitrificans


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  aminotransferase zp00628577

<400>  14
atg aac caa ccg caa agc tgg gaa gcc cgg gcc gag acc tat tcg ctc       48
Met Asn Gln Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu         
1               5                   10                  15              

tac ggt ttc acc gac atg ccc tcg gtc cat cag cgg ggc acg gtc gtc       96
Tyr Gly Phe Thr Asp Met Pro Ser Val His Gln Arg Gly Thr Val Val         
            20                  25                  30                  

gtg acc cat ggc gag ggg ccc tat atc gtc gat gtc cat ggc cgc cgc      144
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg         
        35                  40                  45                      

tat ctg gat gcc aat tcg ggc ctg tgg aac atg gtc gcg ggc ttc gac      192
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp         
    50                  55                  60                          

cac aag ggc ctg atc gag gcc gcc aag gcg caa tac gac cgc ttt ccc      240
His Lys Gly Leu Ile Glu Ala Ala Lys Ala Gln Tyr Asp Arg Phe Pro         
65                  70                  75                  80          

ggc tat cac gcc ttt ttc ggc cgc atg tcc gac cag acc gtg atg ctg      288
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu         
                85                  90                  95              

tcg gaa aag ctg gtc gag gtc tcg cca ttc gac aac ggc cgg gtc ttc      336
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Asn Gly Arg Val Phe         
            100                 105                 110                 

tat acc aat tcc ggc tcc gag gcg aac gac acc atg gtc aag atg ctg      384
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu         
        115                 120                 125                     

tgg ttc ctg cat gcc gcc gag ggc aag ccg caa aag cgc aag atc ctg      432
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu         
    130                 135                 140                         

acg cgc tgg aac gcc tat cac ggc gtg acc gcg gtt tcg gcc tcg atg      480
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met         
145                 150                 155                 160         

acc ggc aag ccc tac aac tcg gtc ttc ggc ctg ccg ctg ccc ggc ttc      528
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe         
                165                 170                 175             

atc cac ctg acc tgc ccg cat tac tgg cgc tat ggc gag gaa ggc gag      576
Ile His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu         
            180                 185                 190                 

acc gag gcg caa ttc gtc gcc cgc ctg gca cgc gag ctt gag gat acc      624
Thr Glu Ala Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Asp Thr         
        195                 200                 205                     

atc acc cgc gag ggc gcc gac acc atc gcc ggc ttc ttc gcc gag ccg      672
Ile Thr Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro         
    210                 215                 220                         

gtg atg ggc gcg ggg ggg gtg atc ccg ccg gcg aag ggt tat ttc cag      720
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln         
225                 230                 235                 240         

gcc atc ctg ccg atc ttg cgc aag tat gac atc ccg atg atc tcg gac      768
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Met Ile Ser Asp         
                245                 250                 255             

gag gtg atc tgc ggc ttc ggg cgc acc ggc aac acc tgg ggc tgc ctg      816
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Leu         
            260                 265                 270                 

acc tac gac ttc atg ccc gat gcg atc atc tcg tcc aag aac ctg act      864
Thr Tyr Asp Phe Met Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr         
        275                 280                 285                     

gcg ggc ttc ttc ccg atg ggc gcc gtc atc ctc ggg ccc gac ctc gcc      912
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Asp Leu Ala         
    290                 295                 300                         

aag cgg gtc gag gcc gcg gtc gag gcg atc gag gag ttc ccg cac ggc      960
Lys Arg Val Glu Ala Ala Val Glu Ala Ile Glu Glu Phe Pro His Gly         
305                 310                 315                 320         

ttc acc gcc tcg ggc cat ccg gtc ggc tgc gcc atc gcg ctg aag gcc     1008
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala         
                325                 330                 335             

atc gac gtg gtg atg aac gag ggg ctg gcc gag aat gtc cgc cgc ctc     1056
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu         
            340                 345                 350                 

gca ccc cgc ttc gag gcg ggg ctg aag cgc atc gcc gac cgc ccg aac     1104
Ala Pro Arg Phe Glu Ala Gly Leu Lys Arg Ile Ala Asp Arg Pro Asn         
        355                 360                 365                     

atc ggc gaa tac cgc ggc atc ggc ttc atg tgg gcg ctg gag gcg gtc     1152
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val         
    370                 375                 380                         

aag gac aag ccg acc aag acc ccc ttc gac gcc aat ctt tcg gtc agc     1200
Lys Asp Lys Pro Thr Lys Thr Pro Phe Asp Ala Asn Leu Ser Val Ser         
385                 390                 395                 400         

gag cgc atc gcc aat acc tgc acc gat ctg ggg ctg atc tgc cgg ccg     1248
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro         
                405                 410                 415             

ctg ggc cag tcc atc gtg ctg tgc ccg ccc ttc atc ctg acc gag gcg     1296
Leu Gly Gln Ser Ile Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala         
            420                 425                 430                 

cag atg gac gag atg ttc gaa aag ctg gaa aag gcg ctc gac aag gtc     1344
Gln Met Asp Glu Met Phe Glu Lys Leu Glu Lys Ala Leu Asp Lys Val         
        435                 440                 445                     

ttt gcc gag gtg gcc tga                                             1362
Phe Ala Glu Val Ala                                                     
    450                                                                 


<210>  15
<211>  453
<212>  PRT
<213>  Paracoccus denitrificans

<400>  15

Met Asn Gln Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Gly Phe Thr Asp Met Pro Ser Val His Gln Arg Gly Thr Val Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Glu Ala Ala Lys Ala Gln Tyr Asp Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Asn Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Ile His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Ala Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Asp Thr 
        195                 200                 205             


Ile Thr Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Met Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Leu 
            260                 265                 270         


Thr Tyr Asp Phe Met Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Asp Leu Ala 
    290                 295                 300                 


Lys Arg Val Glu Ala Ala Val Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 


Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Ala Gly Leu Lys Arg Ile Ala Asp Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Pro Thr Lys Thr Pro Phe Asp Ala Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Ile Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Glu Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  16
<211>  1350
<212>  DNA
<213>  Bacillus weihenstephanensis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  aminotransferase zp01186960

<400>  16
atg caa gcg acg gag caa aca caa agt ttg aaa aaa aca gat gaa aag       48
Met Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys         
1               5                   10                  15              

tac ctt tgg cat gcg atg aga gga gca gcc cct agt cca acg aat tta       96
Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu         
            20                  25                  30                  

att atc aca aaa gca gaa ggg gca tgg gtg acg gat att gat gga aac      144
Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn         
        35                  40                  45                      

cgt tat tta gac ggt atg tcc ggt ctt tgg tgc gtg aat gtt ggg tat      192
Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr         
    50                  55                  60                          

ggt cga aaa gaa ctt gca aga gcg gcg ttt gaa cag ctt gaa gaa atg      240
Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met         
65                  70                  75                  80          

ccg tat ttc cct ctg act caa agt cat gtt cct gct att aaa tta gca      288
Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala         
                85                  90                  95              

gaa aaa ttg aat gaa tgg ctt gat gat gaa tac gtc att ttc ttt tct      336
Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser         
            100                 105                 110                 

aac agt gga tcg gaa gcg aat gaa aca gca ttt aaa att gct cgt caa      384
Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln         
        115                 120                 125                     

tat cat caa caa aaa ggt gat cat gga cgc tat aag ttt att tcc cgc      432
Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg         
    130                 135                 140                         

tac cgc gct tat cac ggt aac tca atg gga gct ctt gca gca aca ggt      480
Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly         
145                 150                 155                 160         

caa gca cag cga aag tat aaa tat gaa cca ctc ggg caa gga ttc ctg      528
Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu         
                165                 170                 175             

cat gta gca ccg cct gat acg tat cga aat cca gag gat gtt cat aca      576
His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr         
            180                 185                 190                 

ctg gca agt gct gag gaa atc gat cgt gtc atg aca tgg gag tta agc      624
Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser         
        195                 200                 205                     

caa aca gta gcc ggt gtg att atg gag cca atc att act ggg ggc gga      672
Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly         
    210                 215                 220                         

att tta atg cct cct gat gga tat atg gga aaa gta aaa gaa att tgc      720
Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys         
225                 230                 235                 240         

gag aag cac ggt gcg ttg ctc att tgt gat gaa gtt ata tgt gga ttt      768
Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe         
                245                 250                 255             

ggc cgg aca ggg aag cca ttt gga ttt atg aat tat ggc gtc aaa cca      816
Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro         
            260                 265                 270                 

gat atc att aca atg gca aaa ggt att aca agt gcg tat ctt cct ttg      864
Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu         
        275                 280                 285                     

tca gca aca gca gtt aga cga gag gtt tat gag gca ttc gta ggt agt      912
Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser         
    290                 295                 300                         

gat gat tat gat cgc ttc cgc cat gta aat acg ttc gga ggg aat cct      960
Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro         
305                 310                 315                 320         

gct gct tgc gct tta gct ttg aag aat tta gaa att atg gag aat gag     1008
Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu         
                325                 330                 335             

aaa ctc att gaa cgt tcc aaa gaa ttg ggt gaa cga ctg tta tat gag     1056
Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu         
            340                 345                 350                 

cta gag gat gta aaa gag cat cca aac gta ggg gat gtt cgc gga aag     1104
Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys         
        355                 360                 365                     

ggc ctt ctt tta ggc att gaa cta gtg gaa gat aag caa aca aaa gaa     1152
Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu         
    370                 375                 380                         

ccg gct tcc att gaa aag atg aac aaa gtc atc aat gct tgt aaa gaa     1200
Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu         
385                 390                 395                 400         

aaa ggt cta att att ggt aaa aat ggt gac act gtc gca ggt tac aat     1248
Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn         
                405                 410                 415             

aat att ttg cag ctt gca cct cca tta agc atc aca gag gaa gac ttt     1296
Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe         
            420                 425                 430                 

act ttt atc gtt aaa aca atg aaa gaa tgt tta tcc cgc att aac ggg     1344
Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly         
        435                 440                 445                     

cag taa                                                             1350
Gln                                                                     
                                                                        


<210>  17
<211>  449
<212>  PRT
<213>  Bacillus weihenstephanensis

<400>  17

Met Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys 
1               5                   10                  15      


Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu 
            20                  25                  30          


Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn 
        35                  40                  45              


Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr 
    50                  55                  60                  


Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met 
65                  70                  75                  80  


Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala 
                85                  90                  95      


Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser 
            100                 105                 110         


Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln 
        115                 120                 125             


Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg 
    130                 135                 140                 


Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly 
145                 150                 155                 160 


Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu 
                165                 170                 175     


His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr 
            180                 185                 190         


Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser 
        195                 200                 205             


Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly 
    210                 215                 220                 


Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys 
225                 230                 235                 240 


Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe 
                245                 250                 255     


Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro 
            260                 265                 270         


Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu 
        275                 280                 285             


Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser 
    290                 295                 300                 


Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro 
305                 310                 315                 320 


Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu 
                325                 330                 335     


Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu 
            340                 345                 350         


Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys 
        355                 360                 365             


Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu 
    370                 375                 380                 


Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu 
385                 390                 395                 400 


Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn 
                405                 410                 415     


Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe 
            420                 425                 430         


Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly 
        435                 440                 445             


Gln 
    


<210>  18
<211>  1350
<212>  DNA
<213>  artificial

<220>
<223>  coodon-optimized sequence of aminotransferase zp01186960

<400>  18
atgcaggcta ccgaacaaac ccaatctctg aaaaagactg acgaaaaata tctgtggcac     60

gcgatgcgcg gtgcagctcc gtctccgacc aacctgatta ttaccaaagc tgaaggcgcg    120

tgggtgaccg acattgacgg taaccgttat ctggatggca tgagcggcct gtggtgtgtt    180

aatgtcggtt atggccgtaa ggagctggcg cgcgcggcat ttgaacaact ggaagaaatg    240

ccgtacttcc cgctgactca aagccatgtg ccggctatca aactggcgga aaaactgaac    300

gaatggctgg acgacgaata cgtgattttc ttctctaatt ctggctccga agcaaacgaa    360

accgcattca aaatcgcccg tcaatatcac cagcagaaag gtgaccacgg ccgctataaa    420

ttcatcagcc gttatcgtgc ataccatggt aattctatgg gtgcgctggc tgctaccggt    480

caggctcagc gcaaatacaa gtacgaaccg ctgggtcagg gttttctgca cgttgcacca    540

ccggatacct accgtaaccc ggaagacgtc cacaccctgg cttctgccga agaaatcgat    600

cgtgttatga cctgggagct gtcccagact gttgcgggtg ttatcatgga acctattatt    660

accggtggtg gcattctgat gccgccggac ggttatatgg gtaaagtcaa ggaaatctgc    720

gaaaaacacg gcgcgctgct gatctgcgat gaagttatct gtggcttcgg tcgcaccggc    780

aaaccatttg gcttcatgaa ttatggcgta aaacctgaca ttattaccat ggctaaaggc    840

attacttccg cttatctgcc gctgagcgcg accgcagttc gccgcgaagt ttatgaagcg    900

tttgttggtt ctgatgatta cgaccgtttc cgtcatgtaa acacgtttgg cggtaaccca    960

gcggcatgtg cgctggcgct gaaaaacctg gaaatcatgg aaaacgaaaa gctgatcgaa   1020

cgtagcaaag aactgggtga acgtctgctg tacgaactgg aagatgtcaa agaacacccg   1080

aacgtgggcg atgttcgcgg taaaggcctg ctgctgggta ttgaactggt tgaagacaaa   1140

cagaccaagg aaccggcttc cattgaaaag atgaacaaag tgattaacgc gtgcaaagag   1200

aaaggcctga tcattggtaa gaacggtgat accgtggcag gttataacaa cattctgcag   1260

ctggcgccgc ctctgagcat cactgaagaa gatttcacct tcatcgtcaa aactatgaag   1320

gagtgcctga gccgcatcaa tggtcagtaa                                    1350


<210>  19
<211>  300
<212>  PRT
<213>  Bacillus amyloliquefaciens


<220>
<221>  mat_peptide
<222>  (1)..(300)
<223>  Protease B

<400>  19

Ala Ala Thr Thr Gly Thr Gly Thr Thr Leu Lys Gly Lys Thr Val Ser 
1               5                   10                  15      


Leu Asn Ile Ser Ser Glu Ser Gly Lys Tyr Val Leu Arg Asp Leu Ser 
            20                  25                  30          


Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg Glu 
        35                  40                  45              


Tyr Asn Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Asn Gln Phe Thr 
    50                  55                  60                  


Thr Ser Ser Gln Arg Ala Ala Val Asp Ala His Tyr Asn Leu Gly Lys 
65                  70                  75                  80  


Val Tyr Asp Tyr Phe Tyr Gln Lys Phe Asn Arg Asn Ser Tyr Asp Asn 
                85                  90                  95      


Lys Gly Gly Lys Ile Val Ser Ser Val His Tyr Gly Ser Arg Tyr Asn 
            100                 105                 110         


Asn Ala Ala Trp Ile Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp Gly 
        115                 120                 125             


Ser Phe Phe Ser Pro Leu Ser Gly Ser Met Asp Val Thr Ala His Glu 
    130                 135                 140                 


Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu Asn Tyr Glu Asn 
145                 150                 155                 160 


Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr Phe 
                165                 170                 175     


Asn Asp Thr Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Val Ser Gln 
            180                 185                 190         


Pro Ala Leu Arg Ser Leu Ser Asn Pro Thr Lys Tyr Gly Gln Pro Asp 
        195                 200                 205             


Asn Phe Lys Asn Tyr Lys Asn Leu Pro Asn Thr Asp Ala Gly Asp Tyr 
    210                 215                 220                 


Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr Asn 
225                 230                 235                 240 


Thr Ile Thr Lys Ile Gly Val Asn Lys Ala Glu Gln Ile Tyr Tyr Arg 
                245                 250                 255     


Ala Leu Thr Val Tyr Leu Thr Pro Ser Ser Thr Phe Lys Asp Ala Lys 
            260                 265                 270         


Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Gln Asp Ala 
        275                 280                 285             


Ala Ser Val Glu Ala Ala Trp Asn Ala Val Gly Leu 
    290                 295                 300 


<210>  20
<211>  274
<212>  PRT
<213>  Bacillus licheniformis


<220>
<221>  mat_peptide
<222>  (1)..(274)
<223>  Delvolase

<400>  20

Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      


Gln Ala Gln Gly Phe Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  


Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  


Leu Gly Val Ala Pro Ser Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      


Ser Ser Gly Ser Gly Thr Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         


Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             


Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 


Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 


Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     


Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Phe Ser Ser Val Gly 
            180                 185                 190         


Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             


Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 


His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 


Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     


Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         


Ala Gln 
        


<210>  21
<211>  1647
<212>  DNA
<213>  Sus scrofa


<220>
<221>  CDS
<222>  (1)..(1647)
<223>  alternative pig liver esterase

<400>  21
ggg cag cca gcc tcg ccg cct gtt gtg gac act gcc cag ggc cga gtc       48
Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val         
1               5                   10                  15              

ctg ggg aag tac gtc agc tta gaa ggc ctg gca cag ccg gtg gcc gtc       96
Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val         
            20                  25                  30                  

ttc ctg gga gtc cct ttt gcc aag ccc cct ctc gga tcc ttg agg ttt      144
Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe         
        35                  40                  45                      

gct ccg ccg cag cct gca gaa cca tgg agc ttc gtg aag aac acc acc      192
Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr         
    50                  55                  60                          

tcc tac cct ccc atg tgc tgc caa gag cca att ggg gga cag atg ctc      240
Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly Gly Gln Met Leu         
65                  70                  75                  80          

tca gat cta ttt acc aac aga aag gag agg ctc att ccg gag ttt tct      288
Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile Pro Glu Phe Ser         
                85                  90                  95              

gaa gac tgt ctc tac cta aat att tac acc cct gct gac ctg aca aag      336
Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys         
            100                 105                 110                 

agg ggc aga ctg ccg gtg atg gtg tgg atc cac gga gga ggt ctg gtg      384
Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val         
        115                 120                 125                     

gtg ggc ggg gct tcc acc tat gat gga ctg gcc ctc gct gcg cat gaa      432
Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu         
    130                 135                 140                         

aac gtg gtg gtg gtg gcc atc cag tac cgc ctg ggc atc tgg gga ttc      480
Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe         
145                 150                 155                 160         

ttc agc aca ggg gac gaa cac agc cgg ggc aac tgg ggt cac ttg gac      528
Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp         
                165                 170                 175             

cag gtg gcc gca ctg cac tgg gtc cag gag aac atc gcc aac ttt gga      576
Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly         
            180                 185                 190                 

ggc gac cca ggc tct gtg acc atc ttt gga gag tca gca gga ggg gaa      624
Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu         
        195                 200                 205                     

agt gtc tct gtt ctg gtg ttg tct ccc ttg gcc aag aac ctc ttc cac      672
Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His         
    210                 215                 220                         

cgg gcc atc tct gag agt ggc gtg gcc ttc act gct ggc ctg gtc agg      720
Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg         
225                 230                 235                 240         

aag gac atg aag gct gca gct aag caa att gct gtc ctt gct ggg tgt      768
Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys         
                245                 250                 255             

aaa acc acc acc tcg gct gtc ttt gtt cac tgc ctg cgc cag aag tcg      816
Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser         
            260                 265                 270                 

gag gac gag ctc ttg gac tta acg ctg aag atg aaa ttt ttc gct ctt      864
Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Phe Ala Leu         
        275                 280                 285                     

gat ttg cat gga gac ccc aga gag agc cat ccc ttc ctg acc act gtg      912
Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val         
    290                 295                 300                         

gtg gat gga gtg ctg ctg ccc aag atg cct gaa gag att ctg gct gaa      960
Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu         
305                 310                 315                 320         

aag gat ttc aac act gtc ccc tac atc gtg gga atc aac aag caa gag     1008
Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu         
                325                 330                 335             

ttt ggc tgg ctt ctg cca acg atg atg ggc ttc ccc ctc tct gaa ggc     1056
Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly         
            340                 345                 350                 

aag ctg gac cag aag acg gcc acg tca ctc ctg tgg aag tcc tac ccc     1104
Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro         
        355                 360                 365                     

atc gct aac atc cct gag gaa ctg act cca gtg gcc act gac aag tat     1152
Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr         
    370                 375                 380                         

ttg ggg ggg aca gac gac ccc gtc aaa aag aaa gac ctg ttc ctg gac     1200
Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp         
385                 390                 395                 400         

ttg atg ggg gat gtg gtg ttt ggt gtc cca tct gtg acg gtg gcc cgt     1248
Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg         
                405                 410                 415             

caa cac aga gat gca gga gcc ccc acc tac atg tat gag ttt cag tat     1296
Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr         
            420                 425                 430                 

cgc cca agc ttc tca tcg gac aag aaa ccc aag acg gtg atc ggg gac     1344
Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp         
        435                 440                 445                     

cac ggg gat gag atc ttc tcc gtc ttt ggt ttt cca ctg tta aaa ggc     1392
His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly         
    450                 455                 460                         

gat gcc cca gaa gag gag gtc agt ctc agc aag acg gtg atg aaa ttc     1440
Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe         
465                 470                 475                 480         

tgg gcc aac ttt gct cgc agt ggg aac ccc aat ggg gag ggg ctg ccc     1488
Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro         
                485                 490                 495             

cat tgg ccg atg tac gac cag gaa gaa ggg tac ctt cag atc ggc gtc     1536
His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val         
            500                 505                 510                 

aac acc cag gca gcc aag agg ctg aaa ggt gaa gaa gtg gcc ttc tgg     1584
Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp         
        515                 520                 525                     

aac gat ctc ctg tcc aag gag gca gca aag aag cca ccc aag ata aag     1632
Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys         
    530                 535                 540                         

cat gct gag ctg tga                                                 1647
His Ala Glu Leu                                                         
545                                                                     


<210>  22
<211>  548
<212>  PRT
<213>  Sus scrofa

<400>  22

Gly Gln Pro Ala Ser Pro Pro Val Val Asp Thr Ala Gln Gly Arg Val 
1               5                   10                  15      


Leu Gly Lys Tyr Val Ser Leu Glu Gly Leu Ala Gln Pro Val Ala Val 
            20                  25                  30          


Phe Leu Gly Val Pro Phe Ala Lys Pro Pro Leu Gly Ser Leu Arg Phe 
        35                  40                  45              


Ala Pro Pro Gln Pro Ala Glu Pro Trp Ser Phe Val Lys Asn Thr Thr 
    50                  55                  60                  


Ser Tyr Pro Pro Met Cys Cys Gln Glu Pro Ile Gly Gly Gln Met Leu 
65                  70                  75                  80  


Ser Asp Leu Phe Thr Asn Arg Lys Glu Arg Leu Ile Pro Glu Phe Ser 
                85                  90                  95      


Glu Asp Cys Leu Tyr Leu Asn Ile Tyr Thr Pro Ala Asp Leu Thr Lys 
            100                 105                 110         


Arg Gly Arg Leu Pro Val Met Val Trp Ile His Gly Gly Gly Leu Val 
        115                 120                 125             


Val Gly Gly Ala Ser Thr Tyr Asp Gly Leu Ala Leu Ala Ala His Glu 
    130                 135                 140                 


Asn Val Val Val Val Ala Ile Gln Tyr Arg Leu Gly Ile Trp Gly Phe 
145                 150                 155                 160 


Phe Ser Thr Gly Asp Glu His Ser Arg Gly Asn Trp Gly His Leu Asp 
                165                 170                 175     


Gln Val Ala Ala Leu His Trp Val Gln Glu Asn Ile Ala Asn Phe Gly 
            180                 185                 190         


Gly Asp Pro Gly Ser Val Thr Ile Phe Gly Glu Ser Ala Gly Gly Glu 
        195                 200                 205             


Ser Val Ser Val Leu Val Leu Ser Pro Leu Ala Lys Asn Leu Phe His 
    210                 215                 220                 


Arg Ala Ile Ser Glu Ser Gly Val Ala Phe Thr Ala Gly Leu Val Arg 
225                 230                 235                 240 


Lys Asp Met Lys Ala Ala Ala Lys Gln Ile Ala Val Leu Ala Gly Cys 
                245                 250                 255     


Lys Thr Thr Thr Ser Ala Val Phe Val His Cys Leu Arg Gln Lys Ser 
            260                 265                 270         


Glu Asp Glu Leu Leu Asp Leu Thr Leu Lys Met Lys Phe Phe Ala Leu 
        275                 280                 285             


Asp Leu His Gly Asp Pro Arg Glu Ser His Pro Phe Leu Thr Thr Val 
    290                 295                 300                 


Val Asp Gly Val Leu Leu Pro Lys Met Pro Glu Glu Ile Leu Ala Glu 
305                 310                 315                 320 


Lys Asp Phe Asn Thr Val Pro Tyr Ile Val Gly Ile Asn Lys Gln Glu 
                325                 330                 335     


Phe Gly Trp Leu Leu Pro Thr Met Met Gly Phe Pro Leu Ser Glu Gly 
            340                 345                 350         


Lys Leu Asp Gln Lys Thr Ala Thr Ser Leu Leu Trp Lys Ser Tyr Pro 
        355                 360                 365             


Ile Ala Asn Ile Pro Glu Glu Leu Thr Pro Val Ala Thr Asp Lys Tyr 
    370                 375                 380                 


Leu Gly Gly Thr Asp Asp Pro Val Lys Lys Lys Asp Leu Phe Leu Asp 
385                 390                 395                 400 


Leu Met Gly Asp Val Val Phe Gly Val Pro Ser Val Thr Val Ala Arg 
                405                 410                 415     


Gln His Arg Asp Ala Gly Ala Pro Thr Tyr Met Tyr Glu Phe Gln Tyr 
            420                 425                 430         


Arg Pro Ser Phe Ser Ser Asp Lys Lys Pro Lys Thr Val Ile Gly Asp 
        435                 440                 445             


His Gly Asp Glu Ile Phe Ser Val Phe Gly Phe Pro Leu Leu Lys Gly 
    450                 455                 460                 


Asp Ala Pro Glu Glu Glu Val Ser Leu Ser Lys Thr Val Met Lys Phe 
465                 470                 475                 480 


Trp Ala Asn Phe Ala Arg Ser Gly Asn Pro Asn Gly Glu Gly Leu Pro 
                485                 490                 495     


His Trp Pro Met Tyr Asp Gln Glu Glu Gly Tyr Leu Gln Ile Gly Val 
            500                 505                 510         


Asn Thr Gln Ala Ala Lys Arg Leu Lys Gly Glu Glu Val Ala Phe Trp 
        515                 520                 525             


Asn Asp Leu Leu Ser Lys Glu Ala Ala Lys Lys Pro Pro Lys Ile Lys 
    530                 535                 540                 


His Ala Glu Leu 
545             


<210>  23
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer for aminotransferase of Pseudomonas aerogenosa 
       gi9946143

<400>  23
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaaca gccaaatcac     60

caacgccaag                                                            70


<210>  24
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer for aminotransferase of Pseudomonas aeruginosa 
       gi9946143

<400>  24
ggggaccact ttgtacaaga aagctgggtt caagccagga cggcggcgg                 49


<210>  25
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer for aminotransferase of Pseudomonas aeruginosa 
       gi9951072

<400>  25
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaacg caagactgca     60

cgccac                                                                66


<210>  26
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer for aminotransferase of Pseudomonas aeruginosa 
       gi9951072

<400>  26
ggggaccact ttgtacaaga aagctgggtt taccggtgac cggcgcgg                  48


