                               SEQUENCE LISTING

<110> PEREIRA, SUZETTE
      DAS, TAPAS
      KRISHNAN, PADMAVATHY
      MUKERJI, PRADIP
 
<120> NOVEL DELTA9-ELONGASE FOR PRODUCTION OF POLYUNSATURATED 
      FATTY ACID-ENRICHED OILS

<130> 28072-58 (9914USL1)

<140>
<141>

<160> 32    

<170> PatentIn version 3.5

<210> 1
<211> 302
<212> PRT
<213> Pavlova salina

<400> 1
Met Lys Ala Ala Ala Gly Lys Val Gln Gln Glu Ala Glu Arg Leu Thr 
1               5                   10                  15      


Ala Gly Leu Trp Leu Pro Met Met Leu Ala Ala Gly Tyr Leu Leu Val 
            20                  25                  30          


Leu Ser Ala Asn Arg Ala Ser Phe Tyr Glu Asn Ile Asn Asn Glu Lys 
        35                  40                  45              


Gly Ala Tyr Ser Thr Ser Trp Phe Ser Leu Pro Cys Val Met Thr Ala 
    50                  55                  60                  


Val Tyr Leu Gly Gly Val Phe Gly Leu Thr Lys Tyr Phe Glu Gly Arg 
65                  70                  75                  80  


Lys Pro Met Gln Gly Leu Lys Asp Tyr Met Phe Thr Tyr Asn Leu Tyr 
                85                  90                  95      


Gln Val Ile Ile Asn Val Trp Cys Ile Ala Ala Phe Val Val Glu Val 
            100                 105                 110         


Arg Arg Ala Gly Met Ser Ala Val Gly Asn Lys Val Asp Leu Gly Pro 
        115                 120                 125             


Asn Ser Phe Arg Leu Gly Phe Val Thr Trp Val His Tyr Asn Asn Lys 
    130                 135                 140                 


Tyr Val Glu Leu Leu Asp Thr Leu Trp Met Val Leu Arg Lys Lys Thr 
145                 150                 155                 160 


Gln Gln Val Ser Phe Leu His Val Tyr His His Val Leu Leu Ile Trp 
                165                 170                 175     


Ala Trp Phe Cys Val Val Lys Phe Cys Asn Gly Gly Asp Ala Tyr Phe 
            180                 185                 190         


Gly Gly Met Leu Asn Ser Ile Ile His Val Met Met Tyr Ser Tyr Tyr 
        195                 200                 205             


Thr Met Ala Leu Leu Gly Trp Ser Cys Pro Trp Lys Arg Tyr Leu Thr 
    210                 215                 220                 


Gln Ala Gln Leu Val Gln Phe Cys Ile Cys Leu Ala His Ala Thr Trp 
225                 230                 235                 240 


Ala Ala Ala Thr Gly Val Tyr Pro Phe His Ile Cys Leu Val Glu Ile 
                245                 250                 255     


Trp Val Met Val Ser Met Leu Tyr Leu Phe Thr Lys Phe Tyr Asn Ser 
            260                 265                 270         


Ala Tyr Lys Gly Ala Ala Lys Gly Ala Ala Ala Ser Ser Asn Gly Ala 
        275                 280                 285             


Ala Ala Pro Ser Gly Ala Lys Pro Lys Ser Ile Lys Ala Asn 
    290                 295                 300         


<210> 2
<211> 263
<212> PRT
<213> Isochrysis galbana

<400> 2
Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr 
1               5                   10                  15      


Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro 
            20                  25                  30          


Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg 
        35                  40                  45              


Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu 
    50                  55                  60                  


Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly 
65                  70                  75                  80  


Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln 
                85                  90                  95      


Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys 
            100                 105                 110         


Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu 
        115                 120                 125             


Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe 
    130                 135                 140                 


Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly 
145                 150                 155                 160 


Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr 
                165                 170                 175     


Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu 
        195                 200                 205             


Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys 
    210                 215                 220                 


Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu 
225                 230                 235                 240 


Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser 
                245                 250                 255     


Ala Lys Ala Gly Lys Gln Leu 
            260             


<210> 3
<211> 263
<212> PRT
<213> Eutreptiella sp.

<400> 3
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Ala Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210> 4
<211> 258
<212> PRT
<213> Euglena gracialis

<400> 4
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210> 5
<211> 258
<212> PRT
<213> Euglena anabena

<400> 5
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Gln Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210> 6
<211> 744
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide

<400> 6
tttcggtccg gattcccggg aatttttttt cgtgttgcct ggctagtacg cccccctccc       60

tcctgtgacc ctccaccaca caccccccaa aggatggacg tcgcgactac gctggctggc      120

atcgcggcgg acgtgctgcc ccgcgtggac tacgcgcggc ttgggcgcga cgccgccgcc      180

tgcgaggttc tatacctttc gctgttcttc atcgccatga agttcatcct tcgccccctc      240

ggcgacaagg ggcaggcccg cctcaagtcg ctcttcaccc tctacaacct cgtgatgtcc      300

atctactccc tcggatcttt cgttgtaatg ggctacgcct tggcggatat cggagtgctc      360

ggtggtgatt gcgggaaagc attctcaaat cccatgttcc gcctcaccgc tcagttgttc      420

tacatcagca agtacgttga gtacatcgat tccttctacg tgcttctcac caacaagccc      480

ctgacctacc tgcagttctt ccaccacctc ggagcccccg tcgacctctg gctcttcctg      540

cagtacgaaa acgaggcgct gtggatcttc gtcttcctca acggcttcat ccacttcatc      600

atgtacgggt actactgggc ccggctggtg aagctcccgt tccccgtgcc gaagtcgttc      660

atcacctcca tgcagatcat ccagttcaac ctgggcttct acctcgtgtg gcggtaccac      720

acaatcccgt gctaccgaca ggac                                             744


<210> 7
<211> 217
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 7
Met Asp Val Ala Thr Thr Leu Ala Gly Ile Ala Ala Asp Val Leu Pro 
1               5                   10                  15      


Arg Val Asp Tyr Ala Arg Leu Gly Arg Asp Ala Ala Ala Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Leu Phe Phe Ile Ala Met Lys Phe Ile Leu Arg Pro 
        35                  40                  45              


Leu Gly Asp Lys Gly Gln Ala Arg Leu Lys Ser Leu Phe Thr Leu Tyr 
    50                  55                  60                  


Asn Leu Val Met Ser Ile Tyr Ser Leu Gly Ser Phe Val Val Met Gly 
65                  70                  75                  80  


Tyr Ala Leu Ala Asp Ile Gly Val Leu Gly Gly Asp Cys Gly Lys Ala 
                85                  90                  95      


Phe Ser Asn Pro Met Phe Arg Leu Thr Ala Gln Leu Phe Tyr Ile Ser 
            100                 105                 110         


Lys Tyr Val Glu Tyr Ile Asp Ser Phe Tyr Val Leu Leu Thr Asn Lys 
        115                 120                 125             


Pro Leu Thr Tyr Leu Gln Phe Phe His His Leu Gly Ala Pro Val Asp 
    130                 135                 140                 


Leu Trp Leu Phe Leu Gln Tyr Glu Asn Glu Ala Leu Trp Ile Phe Val 
145                 150                 155                 160 


Phe Leu Asn Gly Phe Ile His Phe Ile Met Tyr Gly Tyr Tyr Trp Ala 
                165                 170                 175     


Arg Leu Val Lys Leu Pro Phe Pro Val Pro Lys Ser Phe Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Leu Gly Phe Tyr Leu Val Trp Arg Tyr 
        195                 200                 205             


His Thr Ile Pro Cys Tyr Arg Gln Asp 
    210                 215         


<210> 8
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer 


<220>
<221> modified_base
<222> (57)..(57)
<223> a, c, g or t

<400> 8
aagcagtggt atcaacgcag agtacttttt tttttttttt tttttttttt tttttvn          57


<210> 9
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 9
aggcgctgtg gatcttcgtc ttcc                                              24


<210> 10
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 10
ctaatacgac tcactatagc aagcagtggt atcaacgcag agt                         43


<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 11
ctaatacgac tcactatagg gc                                                22


<210> 12
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 12
tccccgtgcc gaagtcgttc atcacc                                            26


<210> 13
<211> 548
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide 

<400> 13
tccccgtgcc gaagtcgttc atcacctcca tgcagatcat ccagttcaac ctgggcttct       60

acctcgtgtg gcggtaccac actatcccgt gctaccgaca ggacccaatg cgaatgttcg      120

cttggctctt caactacttc tacgtgggag tggtcttact gctgtttttg aatttctacg      180

tgcacacgta cgtgatcaag aaggcgcggc ggctggcgaa ggatgagaag aaagtggact      240

agcggagccg gcggcgccca ctgggacccg gtggctccgt gcgcccttcc tcgcccggca      300

tcgaacccac ccaytccccc actagcctcc acgatactcc cttccctcct ccccagtcca      360

ccgtcgaaag gtatccaggc ccttcgactc acacttgcga ccagatggcg gtttaacctc      420

tgcgcgactc ggagagttgc cctaaccatc tgttctagaa ctcgcgattg gactgtgttg      480

aactggatcc gatgaccctc gtttttccat accgttgtwm aaaaaaaaaa aaaaaaaaaa      540

aaaaaaaa                                                               548


<210> 14
<211> 79
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 14
Pro Val Pro Lys Ser Phe Ile Thr Ser Met Gln Ile Ile Gln Phe Asn 
1               5                   10                  15      


Leu Gly Phe Tyr Leu Val Trp Arg Tyr His Thr Ile Pro Cys Tyr Arg 
            20                  25                  30          


Gln Asp Pro Met Arg Met Phe Ala Trp Leu Phe Asn Tyr Phe Tyr Val 
        35                  40                  45              


Gly Val Val Leu Leu Leu Phe Leu Asn Phe Tyr Val His Thr Tyr Val 
    50                  55                  60                  


Ile Lys Lys Ala Arg Arg Leu Ala Lys Asp Glu Lys Lys Val Asp 
65                  70                  75                  


<210> 15
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 15
caccatggat ccatggacgt cgcgactacg ctgg                                   34


<210> 16
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 16
acgcgtaagc ttctagtcca ctttcttctc atccttc                                37


<210> 17
<211> 789
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide

<400> 17
atggacgtcg cgactacgct ggctggcatc gcggcggacg tgctgccccg cgtggactac       60

gcgcggcttg ggcgcgacgc cgccgcctgc gaggttctat acctttcgct gttcttcatc      120

gccatgaagt tcatccttcg ccccctcggc gacaaggggc aggcccgcct caagtcgctc      180

ttcaccctct acaacctcgt gatgtccatc tactccctcg gatctttcgt tgtaatgggc      240

tacgccttgg cggatatcgg agtgctcggt ggtgattgcg ggaaagcatt ctcaaatccc      300

atgttccgcc tcaccgctca gttgttctac atcagcaagt acgttgagta catcgattcc      360

ttctacgtgc ttctcaccaa caagcccctg acctacctgc agttcttcca ccacctcgga      420

gcccccgtcg acctctggct cttcctgcag tacgaaaacg aggcgctgtg gatcttcgtc      480

ttcctcaacg gcttcatcca cttcatcatg tacgggtact actgggcccg gctggtgaag      540

ctcccgttcc ccgtgccgaa gtcgttcatc acctccatgc agatcatcca gttcaacctg      600

ggcttctacc tcgtgtggcg gtaccacaca atcccgtgct accgacagga cccaatgcga      660

atgttcgctt ggctcttcaa ctacttctac gtgggagtgg tcttactgct gtttttgaat      720

ttctacgtgc acacgtacgt gatcaagaaa gcgcggcggc tggcgaagga tgagaagaaa      780

gtggactag                                                              789


<210> 18
<211> 262
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 18
Met Asp Val Ala Thr Thr Leu Ala Gly Ile Ala Ala Asp Val Leu Pro 
1               5                   10                  15      


Arg Val Asp Tyr Ala Arg Leu Gly Arg Asp Ala Ala Ala Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Leu Phe Phe Ile Ala Met Lys Phe Ile Leu Arg Pro 
        35                  40                  45              


Leu Gly Asp Lys Gly Gln Ala Arg Leu Lys Ser Leu Phe Thr Leu Tyr 
    50                  55                  60                  


Asn Leu Val Met Ser Ile Tyr Ser Leu Gly Ser Phe Val Val Met Gly 
65                  70                  75                  80  


Tyr Ala Leu Ala Asp Ile Gly Val Leu Gly Gly Asp Cys Gly Lys Ala 
                85                  90                  95      


Phe Ser Asn Pro Met Phe Arg Leu Thr Ala Gln Leu Phe Tyr Ile Ser 
            100                 105                 110         


Lys Tyr Val Glu Tyr Ile Asp Ser Phe Tyr Val Leu Leu Thr Asn Lys 
        115                 120                 125             


Pro Leu Thr Tyr Leu Gln Phe Phe His His Leu Gly Ala Pro Val Asp 
    130                 135                 140                 


Leu Trp Leu Phe Leu Gln Tyr Glu Asn Glu Ala Leu Trp Ile Phe Val 
145                 150                 155                 160 


Phe Leu Asn Gly Phe Ile His Phe Ile Met Tyr Gly Tyr Tyr Trp Ala 
                165                 170                 175     


Arg Leu Val Lys Leu Pro Phe Pro Val Pro Lys Ser Phe Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Leu Gly Phe Tyr Leu Val Trp Arg Tyr 
        195                 200                 205             


His Thr Ile Pro Cys Tyr Arg Gln Asp Pro Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Leu Phe Asn Tyr Phe Tyr Val Gly Val Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val His Thr Tyr Val Ile Lys Lys Ala Arg Arg Leu Ala Lys 
                245                 250                 255     


Asp Glu Lys Lys Val Asp 
            260         


<210> 19
<211> 789
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide 

<400> 19
atggacgtcg cgactacgct ggccggcatc gcggcggacg tgctgccccg cgtggactac       60

gcgcggcttg ggcgcgacgc cgtcgcctgc gaggttctat acctttcgct gttcttcatc      120

gccatgaagt tcatccttcg ccccctcggc gacaaggggc aggcccgcct caagtcgctc      180

ttcaccctct acaacctcgt gatgtccatc tactccctcg gatctttcgt tataatgggc      240

tacgccttgg cggatatcgg agtgctcggt ggtgattgcg ggaaagcatt ctcaaatccc      300

ttgttccgca tcaccgctca gttgttctac atcagcaagt acgttgagta catcgattcc      360

ttctacgtgc ttctcaccaa caagcccctg acctacctgc agttcttcca ccacctcgga      420

gcccccgtcg acctctggct cttcctgcag tacgaaaacg aggcgctgtg gatcttcgtc      480

ttcctcaacg gcttcatcca cttcatcatg tacgggtact actgggcccg gctggtgaag      540

ctcccgttcc ccgtgccgaa gtcgttcatc acctccatgc agatcatcca gttcaacctg      600

ggcttctacc tcgtgtggcg gtaccacact atcccgtgct accgacagga cccaatgcga      660

atgttcgctt ggctcttcaa ctacttctac gtgggagtgg tcttactgct gtttttgaat      720

ttctacgtgc acacgtacgt gatcaagaag gcgcggcggc tggcgaagga tgagaagaaa      780

gtggactag                                                              789


<210> 20
<211> 262
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide 

<400> 20
Met Asp Val Ala Thr Thr Leu Ala Gly Ile Ala Ala Asp Val Leu Pro 
1               5                   10                  15      


Arg Val Asp Tyr Ala Arg Leu Gly Arg Asp Ala Val Ala Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Leu Phe Phe Ile Ala Met Lys Phe Ile Leu Arg Pro 
        35                  40                  45              


Leu Gly Asp Lys Gly Gln Ala Arg Leu Lys Ser Leu Phe Thr Leu Tyr 
    50                  55                  60                  


Asn Leu Val Met Ser Ile Tyr Ser Leu Gly Ser Phe Val Ile Met Gly 
65                  70                  75                  80  


Tyr Ala Leu Ala Asp Ile Gly Val Leu Gly Gly Asp Cys Gly Lys Ala 
                85                  90                  95      


Phe Ser Asn Pro Leu Phe Arg Ile Thr Ala Gln Leu Phe Tyr Ile Ser 
            100                 105                 110         


Lys Tyr Val Glu Tyr Ile Asp Ser Phe Tyr Val Leu Leu Thr Asn Lys 
        115                 120                 125             


Pro Leu Thr Tyr Leu Gln Phe Phe His His Leu Gly Ala Pro Val Asp 
    130                 135                 140                 


Leu Trp Leu Phe Leu Gln Tyr Glu Asn Glu Ala Leu Trp Ile Phe Val 
145                 150                 155                 160 


Phe Leu Asn Gly Phe Ile His Phe Ile Met Tyr Gly Tyr Tyr Trp Ala 
                165                 170                 175     


Arg Leu Val Lys Leu Pro Phe Pro Val Pro Lys Ser Phe Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Leu Gly Phe Tyr Leu Val Trp Arg Tyr 
        195                 200                 205             


His Thr Ile Pro Cys Tyr Arg Gln Asp Pro Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Leu Phe Asn Tyr Phe Tyr Val Gly Val Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val His Thr Tyr Val Ile Lys Lys Ala Arg Arg Leu Ala Lys 
                245                 250                 255     


Asp Glu Lys Lys Val Asp 
            260         


<210> 21
<211> 312
<212> PRT
<213> Mus musculus

<400> 21
Met Gly Leu Leu Asp Ser Glu Pro Gly Ser Val Leu Asn Ala Met Ser 
1               5                   10                  15      


Thr Ala Phe Asn Asp Thr Val Glu Phe Tyr Arg Trp Thr Trp Thr Ile 
            20                  25                  30          


Ala Asp Lys Arg Val Ala Asp Trp Pro Leu Met Gln Ser Pro Trp Pro 
        35                  40                  45              


Thr Ile Ser Ile Ser Thr Leu Tyr Leu Leu Phe Val Trp Leu Gly Pro 
    50                  55                  60                  


Lys Trp Met Lys Asp Arg Glu Pro Phe Gln Met Arg Leu Val Leu Ile 
65                  70                  75                  80  


Ile Tyr Asn Phe Gly Met Val Leu Leu Asn Leu Phe Ile Phe Arg Glu 
                85                  90                  95      


Leu Phe Met Gly Ser Tyr Asn Ala Gly Tyr Ser Tyr Ile Cys Gln Ser 
            100                 105                 110         


Val Asp Tyr Ser Asn Asp Val Asn Glu Val Arg Ile Ala Ala Ala Leu 
        115                 120                 125             


Trp Trp Tyr Phe Val Ser Lys Gly Val Glu Tyr Leu Asp Thr Val Phe 
    130                 135                 140                 


Phe Ile Leu Arg Lys Lys Asn Asn Gln Val Ser Phe Leu His Val Tyr 
145                 150                 155                 160 


His His Cys Thr Met Phe Thr Leu Trp Trp Ile Gly Ile Lys Trp Val 
                165                 170                 175     


Ala Gly Gly Gln Ala Phe Phe Gly Ala Gln Met Asn Ser Phe Ile His 
            180                 185                 190         


Val Ile Met Tyr Ser Tyr Tyr Gly Leu Thr Ala Phe Gly Pro Trp Ile 
        195                 200                 205             


Gln Lys Tyr Leu Trp Trp Lys Arg Tyr Leu Thr Met Leu Gln Leu Val 
    210                 215                 220                 


Gln Phe His Val Thr Ile Gly His Thr Ala Leu Ser Leu Tyr Thr Asp 
225                 230                 235                 240 


Cys Pro Phe Pro Lys Trp Met His Trp Ala Leu Ile Ala Tyr Ala Ile 
                245                 250                 255     


Ser Phe Ile Phe Leu Phe Leu Asn Phe Tyr Thr Arg Thr Tyr Asn Glu 
            260                 265                 270         


Pro Lys Gln Ser Lys Thr Gly Lys Thr Ala Thr Asn Gly Ile Ser Ser 
        275                 280                 285             


Asn Gly Val Asn Lys Ser Glu Lys Ala Leu Glu Asn Gly Lys Pro Gln 
    290                 295                 300                 


Lys Asn Gly Lys Pro Lys Gly Glu 
305                 310         


<210> 22
<211> 296
<212> PRT
<213> Homo sapiens

<400> 22
Met Glu His Leu Lys Ala Phe Asp Asp Glu Ile Asn Ala Phe Leu Asp 
1               5                   10                  15      


Asn Met Phe Gly Pro Arg Asp Ser Arg Val Arg Gly Trp Phe Met Leu 
            20                  25                  30          


Asp Ser Tyr Leu Pro Thr Phe Phe Leu Thr Val Met Tyr Leu Leu Ser 
        35                  40                  45              


Ile Trp Leu Gly Asn Lys Tyr Met Lys Asn Arg Pro Ala Leu Ser Leu 
    50                  55                  60                  


Arg Gly Ile Leu Thr Leu Tyr Asn Leu Gly Ile Thr Leu Leu Ser Ala 
65                  70                  75                  80  


Tyr Met Leu Ala Glu Leu Ile Leu Ser Thr Trp Glu Gly Gly Tyr Asn 
                85                  90                  95      


Leu Gln Cys Gln Asp Leu Thr Ser Ala Gly Glu Ala Asp Ile Arg Val 
            100                 105                 110         


Ala Lys Val Leu Trp Trp Tyr Tyr Phe Ser Lys Ser Val Glu Phe Leu 
        115                 120                 125             


Asp Thr Ile Phe Phe Val Leu Arg Lys Lys Thr Ser Gln Ile Thr Phe 
    130                 135                 140                 


Leu His Val Tyr His His Ala Ser Met Phe Asn Ile Trp Trp Cys Val 
145                 150                 155                 160 


Leu Asn Trp Ile Pro Cys Gly Gln Ser Phe Phe Gly Pro Thr Leu Asn 
                165                 170                 175     


Ser Phe Ile His Ile Leu Met Tyr Ser Tyr Tyr Gly Leu Ser Val Phe 
            180                 185                 190         


Pro Ser Met His Lys Tyr Leu Trp Trp Lys Lys Tyr Leu Thr Gln Ala 
        195                 200                 205             


Gln Leu Val Gln Phe Val Leu Thr Ile Thr His Thr Met Ser Ala Val 
    210                 215                 220                 


Val Lys Pro Cys Gly Phe Pro Phe Gly Cys Leu Ile Phe Gln Ser Ser 
225                 230                 235                 240 


Tyr Met Leu Thr Leu Val Ile Leu Phe Leu Asn Phe Tyr Val Gln Thr 
                245                 250                 255     


Tyr Arg Lys Lys Pro Met Lys Lys Asp Met Gln Glu Pro Pro Ala Gly 
            260                 265                 270         


Lys Glu Val Lys Asn Gly Phe Ser Lys Ala Tyr Phe Thr Ala Ala Asn 
        275                 280                 285             


Gly Val Met Asn Lys Lys Ala Gln 
    290                 295     


<210> 23
<211> 288
<212> PRT
<213> Caenorhabditis elegans

<400> 23
Met Ala Gln His Pro Leu Val Gln Arg Leu Leu Asp Val Lys Phe Asp 
1               5                   10                  15      


Thr Lys Arg Phe Val Ala Ile Ala Thr His Gly Pro Lys Asn Phe Pro 
            20                  25                  30          


Asp Ala Glu Gly Arg Lys Phe Phe Ala Asp His Phe Asp Val Thr Ile 
        35                  40                  45              


Gln Ala Ser Ile Leu Tyr Met Val Val Val Phe Gly Thr Lys Trp Phe 
    50                  55                  60                  


Met Arg Asn Arg Gln Pro Phe Gln Leu Thr Ile Pro Leu Asn Ile Trp 
65                  70                  75                  80  


Asn Phe Ile Leu Ala Ala Phe Ser Ile Ala Gly Ala Val Lys Met Thr 
                85                  90                  95      


Pro Glu Phe Phe Gly Thr Ile Ala Asn Lys Gly Ile Val Ala Ser Tyr 
            100                 105                 110         


Cys Lys Val Phe Asp Phe Thr Lys Gly Glu Asn Gly Tyr Trp Val Trp 
        115                 120                 125             


Leu Phe Met Ala Ser Lys Leu Phe Glu Leu Val Asp Thr Ile Phe Leu 
    130                 135                 140                 


Val Leu Arg Lys Arg Pro Leu Met Phe Leu His Trp Tyr His His Ile 
145                 150                 155                 160 


Leu Thr Met Ile Tyr Ala Trp Tyr Ser His Pro Leu Thr Pro Gly Phe 
                165                 170                 175     


Asn Arg Tyr Gly Ile Tyr Leu Asn Phe Val Val His Ala Phe Met Tyr 
            180                 185                 190         


Ser Tyr Tyr Phe Leu Arg Ser Met Lys Ile Arg Val Pro Gly Phe Ile 
        195                 200                 205             


Ala Gln Ala Ile Thr Ser Leu Gln Ile Val Gln Phe Ile Ile Ser Cys 
    210                 215                 220                 


Ala Val Leu Ala His Leu Gly Tyr Leu Met His Phe Thr Asn Ala Asn 
225                 230                 235                 240 


Cys Asp Phe Glu Pro Ser Val Phe Lys Leu Ala Val Phe Met Asp Thr 
                245                 250                 255     


Thr Tyr Leu Ala Leu Phe Val Asn Phe Phe Leu Gln Ser Tyr Val Leu 
            260                 265                 270         


Arg Gly Gly Lys Asp Lys Tyr Lys Ala Val Pro Lys Lys Lys Asn Asn 
        275                 280                 285             


<210> 24
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 24
tatagaattc aaatggacgt cgcgactacg ctg                                    33


<210> 25
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 25
tattctcgag ttctagtcca ctttcttctc atccttc                                37


<210> 26
<211> 801
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide 

<400> 26
ggatccatgg acgtcgcgac tacgctggct ggcatcgcgg cggacgtgct gccccgcgtg       60

gactacgcgc ggcttgggcg cgacgccgcc gcctgcgagg ttctatacct ttcgctgttc      120

ttcatcgcca tgaagttcat ccttcgcccc ctcggcgaca aggggcaggc ccgcctcaag      180

tcgctcttca ccctctacaa cctcgtgatg tccatctact ccctcggatc tttcgttgta      240

atgggctacg ccttggcgga tatcggagtg ctcggtggtg attgcgggaa agcattctca      300

aatcccatgt tccgcctcac cgctcagttg ttctacatca gcaagtacgt tgagtacatc      360

gattccttct acgtgcttct caccaacaag cccctgacct acctgcagtt cttccaccac      420

ctcggagccc ccgtcgacct ctggctcttc ctgcagtacg aaaacgaggc gctgtggatc      480

ttcgtcttcc tcaacggctt catccacttc atcatgtacg ggtactactg ggcccggctg      540

gtgaagctcc cgttccccgt gccgaagtcg ttcatcacct ccatgcagat catccagttc      600

aacctgggct tctacctcgt gtggcggtac cacacaatcc cgtgctaccg acaggaccca      660

atgcgaatgt tcgcttggct cttcaactac ttctacgtgg gagtggtctt actgctgttt      720

ttgaatttct acgtgcacac gtacgtgatc aagaaagcgc ggcggctggc gaaggatgag      780

aagaaagtgg actagaagct t                                                801


<210> 27
<211> 801
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polynucleotide 

<400> 27
ggatccatgg acgtcgcgac tacgctggcc ggcatcgcgg cggacgtgct gccccgcgtg       60

gactacgcgc ggcttgggcg cgacgccgtc gcctgcgagg ttctatacct ttcgctgttc      120

ttcatcgcca tgaagttcat ccttcgcccc ctcggcgaca aggggcaggc ccgcctcaag      180

tcgctcttca ccctctacaa cctcgtgatg tccatctact ccctcggatc tttcgttata      240

atgggctacg ccttggcgga tatcggagtg ctcggtggtg attgcgggaa agcattctca      300

aatcccttgt tccgcatcac cgctcagttg ttctacatca gcaagtacgt tgagtacatc      360

gattccttct acgtgcttct caccaacaag cccctgacct acctgcagtt cttccaccac      420

ctcggagccc ccgtcgacct ctggctcttc ctgcagtacg aaaacgaggc gctgtggatc      480

ttcgtcttcc tcaacggctt catccacttc atcatgtacg ggtactactg ggcccggctg      540

gtgaagctcc cgttccccgt gccgaagtcg ttcatcacct ccatgcagat catccagttc      600

aacctgggct tctacctcgt gtggcggtac cacactatcc cgtgctaccg acaggaccca      660

atgcgaatgt tcgcttggct cttcaactac ttctacgtgg gagtggtctt actgctgttt      720

ttgaatttct acgtgcacac gtacgtgatc aagaaggcgc ggcggctggc gaaggatgag      780

aagaaagtgg actagaagct t                                                801


<210> 28
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide


<220>
<221> MOD_RES
<222> (2)..(3)
<223> Any amino acid

<400> 28
His Xaa Xaa His His 
1               5   


<210> 29
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide 


<220>
<221> MOD_RES
<222> (2)..(3)
<223> Any amino acid

<400> 29
Gln Xaa Xaa His His 
1               5   


<210> 30
<211> 57
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 30
Arg Ser Arg Arg Arg Pro Leu Gly Pro Gly Gly Ser Val Arg Pro Ser 
1               5                   10                  15      


Ser Pro Gly Ile Glu Pro Thr His Ser Pro Thr Ser Leu His Asp Thr 
            20                  25                  30          


Pro Phe Pro Pro Pro Gln Ser Thr Val Glu Arg Tyr Pro Gly Pro Ser 
        35                  40                  45              


Thr His Thr Cys Asp Gln Met Ala Val 
    50                  55          


<210> 31
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 31
Pro Leu Arg Asp Ser Glu Ser Cys Pro Asn His Leu Phe 
1               5                   10              


<210> 32
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide


<220>
<221> MOD_RES
<222> (21)..(21)
<223> Any amino acid

<400> 32
Asn Ser Arg Leu Asp Cys Val Glu Leu Asp Pro Met Thr Leu Val Phe 
1               5                   10                  15      


Pro Tyr Arg Cys Xaa Lys Lys Lys Lys Lys Lys Lys Lys Lys 
            20                  25                  30  


