                         SEQUENCE LISTING

<110>  DNA2.0, Inc
 
<120>  BIOSYNTHETIC ROUTES TO LONG-CHAIN ALPHA,OMEGA-HYDROXYACIDS, 
       DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS

<130>  11548-021-228

<140>  PCT/US10/001362
<141>  2010-05-06

<150>  US 12/436,729
<151>  2009-05-06

<160>  149   

<170>  PatentIn version 3.5

<210>  1
<211>  4186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Contains flp recominase from Saccharomyces cerevisiae with 
       flanking regions as well as the gene encoding resistance to the 
       Nourseothricin resistance marker from transposon Tn1825

<400>  1
ctcgaggaag ttcctatact ttctagagaa taggaacttc ggatccaata atgattggtt       60

tgatattttt gtctagtacc atctgtacca ttacacttaa attatcttta tatctgtcta      120

actcgactgt ctggatttca ttgatgtagt cgtatgcatc gttagttcca aaaaatattg      180

tcatcaattt gatattggtt tccgactcta aaatttttgg aagaatttgt ctagcgtgct      240

ctgagttgta gccactgaaa ccacggttaa taacatccaa ttttcggata tacacattct      300

gtaatgctgg atgaaagcca tactgggtac aactaaactg ggtgatggag tcaccgaaca      360

acacaaattt accgtattcc atgattgcta tggttgagaa tttttttttt ttcttgtccc      420

acgccatttt tcaaattatg cagttgagaa tgttagtttt tgtgtacacc ccgttcgctg      480

aatatttcgg aataattcaa agattgggga gtgggggagg cgatagacga agacacggta      540

taaaaatggg caaaattttc cccaactttt tgcagtggtt taactaataa tcgtcgacat      600

gccacaattt gatatattat gtaaaacacc acctaaggtg cttgttcgtc agtttgtgga      660

aaggtttgaa agaccttcag gtgagaaaat agcattatgt gctgctgaac taacctattt      720

atgttggatg attacacata acggaacagc aatcaagaga gccacattca tgagctataa      780

tactatcata agcaattcgt tgagtttcga tattgtcaat aaatcactcc agtttaaata      840

caagacgcaa aaagcaacaa ttttggaagc ctcattaaag aaattgattc ctgcttggga      900

atttacaatt attccttact atggacaaaa acatcaatct gatatcactg atattgtaag      960

tagtttgcaa ttacagttcg aatcatcgga agaagcagat aagggaaata gccacagtaa     1020

aaaaatgctt aaagcacttc taagtgaggg tgaaagcatc tgggagatca ctgagaaaat     1080

actaaattcg tttgagtata cttcgagatt tacaaaaaca aaaactttat accaattcct     1140

cttcctagct actttcatca attgtggaag attcagcgat attaagaacg ttgatccgaa     1200

atcatttaaa ttagtccaaa ataagtattt gggagtaata atccagtgtt tagtgacaga     1260

gacaaagaca agcgttagta ggcacatata cttctttagc gcaaggggta ggatcgatcc     1320

acttgtatat ttggatgaat ttttgaggaa ttctgaacca gtcctaaaac gagtaaatag     1380

gaccggcaat tcttcaagca ataaacagga ataccaatta ttaaaagata acttagtcag     1440

atcgtacaat aaagctttga agaaaaatgc gccttattca atctttgcta taaaaaatgg     1500

cccaaaatct cacattggaa gacatttgat gacctcattt ctttcaatga agggcctaac     1560

ggagttgact aatgttgtgg gaaattggag cgataagcgt gcttctgccg tggccaggac     1620

aacgtatact catcagataa cagcaatacc tgatcactac ttcgcactag tttctcggta     1680

ctatgcatat gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat     1740

tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc     1800

cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct acataaatag     1860

acgcatataa gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc ttgttattct     1920

tctttttgtt attacatata taacttgtta cttttttaaa aaaatctttg tatattttat     1980

aaatatataa aactaaattt aagaaaaaga gaaaaatgtt ttatttgaga gattgaaatt     2040

ttacttgaat ttagcttagc ttttataaag tattattatg taaaaaaaca aaacaaatat     2100

acattaaaaa gttaagacta taaaatagcc acccaaggca tttctatatc ttgttgttgt     2160

tgttttcatc ttctgtatca gaggaactta ttttattatt ttcgtcacgg gtattttctc     2220

ttgtttgatg attcatccca ttcattccat cataaaatgt cgagcgtcaa aactagagaa     2280

taataaagaa aacgatcttt tcaaaaagaa aaaacctttt agttttcctt tgttgttgtt     2340

gtgggtgtgt gctatttata ttatatagtt tactcataat accataaaat attcggtttg     2400

attaggttat tttaataagc taatttgttt ctaatcgtgt aatttatgct gtgtatatta     2460

agtagtgtgt gcactgccca aaaatgtttg ttgtttatag tcggttaaag agaaaaaaga     2520

aaaaaagatc catacacaca cgttaattag ttgttcaacg taatacactc atattttgtt     2580

cttatttgct ttcggtcgct gttctcacca agatttattg ccaacgaaac aatttttttt     2640

tatatatttt cagatttttc tttttttcct ttcctttcct tttctaattt tcactcctgg     2700

ttttctttct ttcttagaaa cattatctcg atattaatat taaaaaaata taatcattca     2760

aaatggacgg tggtatgttt tagtttagct tcaattctaa ttgattgatt aatcagttga     2820

ttggtttcaa tatgacaaat gggtagggtg ggaaaacttc attttcaatt cagatcaaac     2880

ttttttgttg tcgacataat atttctcgtt tgggatgtta ctgtcacatt aataatacac     2940

acacatcagc ttataatttt gaaagtaatt tatcagatat gttgtgacga tcaatggaaa     3000

tggctaactt caatgtatct gttcttcccc tttttcaaag ttcacgtttt ttgattgatt     3060

gattgatctg tcggcagtgg tttcaaaacc attcggtgag taatcctatc aatcaatgtt     3120

acgacaaaag gctcaatatt caaaattgca atgttttatg ttttcctacg tgtacttgtg     3180

caaggcaatt gattcaacat tgcttttggt gtttgacgag tttctagttt ggacttgtgt     3240

tgttatctgg gctatacaga tttcccggct cactatgaat tttttttttc gacgctcagt     3300

gcacacaact ataaacaaca caaacacaaa cacagcaaga aaaaaaaaaa acgaacattg     3360

aattgaaacc aagccaactg aaaaattcct tatttaaatg actgtcatac taacccattt     3420

ttatagaaga agttgctgct ttagttatcg ataacggttc tcatatgaaa atttcggtga     3480

tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt     3540

tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc     3600

ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca     3660

tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta     3720

tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg     3780

aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa     3840

cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg     3900

acctcttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact     3960

ggttctcggg agcacaggat gacgcctaac atatgtgaag tgtgaagggg gagattttca     4020

ctttattaga tttgtatata tgtataataa ataaataaat aagttaaata aataattaga     4080

taagggtggt aattattact atttacaatc aaaggtggtc ctgcaggaag ttcctatact     4140

ttctagagaa taggaacttc agatccacta gttctagagc ggccgc                    4186


<210>  2
<211>  3826
<212>  DNA
<213>  Candida tropicalis

<400>  2
tggagtcgcc agacttgctc acttttgact cccttcgaaa ctcaaagtac gttcaggcgg       60

tgctcaacga aacgctccgt atctacccgg gggtaccacg aaacatgaag acagctacgt      120

gcaacacgac gttgccacgc ggaggaggca aagacggcaa ggaacctatc ttggtgcaga      180

agggacagtc cgttgggttg attactattg ccacgcagac ggacccagag tattttgggg      240

ccgacgctgg tgagtttaag ccggagagat ggtttgattc aagcatgaag aacttggggt      300

gtaaatactt gccgttcaat gctgggccac ggacttgctt ggggcagcag tacactttga      360

ttgaagcgag ctacttgcta gtccggttgg cccagaccta ccgggcaata gatttgcagc      420

caggatcggc gtacccacca agaaagaagt cgttgatcaa catgagtgct gccgacgggg      480

tgtttgtaaa gctttataag gatgtaacgg tagatggata gttgtgtagg aggagcggag      540

ataaattaga tttgattttg tgtaaggttt tggatgtcaa cctactccgc acttcatgca      600

gtgtgtgtga cacaagggtg tactacgtgt gcgtgtgcgc caagagacag cccaaggggg      660

tggtagtgtg tgttggcgga agtgcatgtg acacaacgcg tgggttctgg ccaatggtgg      720

actaagtgca ggtaagcagc gacctgaaac attcctcaac gcttaagaca ctggtggtag      780

agatgcggac caggctattc ttgtcgtgct acccggcgca tggaaaatca actgcgggaa      840

gaataaattt atccgtagaa tccacagagc ggataaattt gcccacctcc atcatcaacc      900

acgccgccac taactacatc actcccctat tttctctctc tctctttgtc ttactccgct      960

cccgtttcct tagccacaga tacacaccca ctgcaaacag cagcaacaat tataaagata     1020

cgccaggccc accttctttc tttttcttca cttttttgac tgcaactttc tacaatccac     1080

cacagccacc accacagccg ctatgattga acaactccta gaatattggt atgtcgttgt     1140

gccagtgttg tacatcatca aacaactcct tgcatacaca aagactcgcg tcttgatgaa     1200

aaagttgggt gctgctccag tcacaaacaa gttgtacgac aacgctttcg gtatcgtcaa     1260

tggatggaag gctctccagt tcaagaaaga gggcagggct caagagtaca acgattacaa     1320

gtttgaccac tccaagaacc caagcgtggg cacctacgtc agtattcttt tcggcaccag     1380

gatcgtcgtg accaaagatc cagagaatat caaagctatt ttggcaaccc agtttggtga     1440

tttttctttg ggcaagaggc acactctttt taagcctttg ttaggtgatg ggatcttcac     1500

attggacggc gaaggctgga agcacagcag agccatgttg agaccacagt ttgccagaga     1560

acaagttgct catgtgacgt cgttggaacc acacttccag ttgttgaaga agcatattct     1620

taagcacaag ggtgaatact ttgatatcca ggaattgttc tttagattta ccgttgattc     1680

ggccacggag ttcttatttg gtgagtccgt gcactcctta aaggacgaat ctattggtat     1740

caaccaagac gatatagatt ttgctggtag aaaggacttt gctgagtcgt tcaacaaagc     1800

ccaggaatac ttggctatta gaaccttggt gcagacgttc tactggttgg tcaacaacaa     1860

ggagtttaga gactgtacca agctggtgca caagttcacc aactactatg ttcagaaagc     1920

tttggatgct agcccagaag agcttgaaaa gcaaagtggg tatgtgttct tgtacgagct     1980

tgtcaagcag acaagagacc ccaatgtgtt gcgtgaccag tctttgaaca tcttgttggc     2040

cggaagagac accactgctg ggttgttgtc gtttgctgtc tttgagttgg ccagacaccc     2100

agagatctgg gccaagttga gagaggaaat tgaacaacag tttggtcttg gagaagactc     2160

tcgtgttgaa gagattacct ttgagagctt gaagagatgt gagtacttga aagcgttcct     2220

taatgaaacc ttgcgtattt acccaagtgt cccaagaaac ttcagaatcg ccaccaagaa     2280

cacgacattg ccaaggggcg gtggttcaga cggtacctcg ccaatcttga tccaaaaggg     2340

agaagctgtg tcgtatggta tcaactctac tcatttggac cctgtctatt acggccctga     2400

tgctgctgag ttcagaccag agagatggtt tgagccatca accaaaaagc tcggctgggc     2460

ttacttgcca ttcaacggtg gtccaagaat ctgtttgggt cagcagtttg ccttgacgga     2520

agctggctat gtgttggtta gattggtgca agagttctcc cacgttaggc tggacccaga     2580

cgaggtgtac ccgccaaaga ggttgaccaa cttgaccatg tgtttgcagg atggtgctat     2640

tgtcaagttt gactagcggc gtggtgaatg cgtttgattt tgtagtttct gtttgcagta     2700

atgagataac tattcagata aggcgagtgg atgtacgttt tgtaagagtt tccttacaac     2760

cttggtgggg tgtgtgaggt tgaggttgca tcttggggag attacacctt ttgcagctct     2820

ccgtatacac ttgtactctt tgtaacctct atcaatcatg tggggggggg ggttcattgt     2880

ttggccatgg tggtgcatgt taaatccgcc aactacccaa tctcacatga aactcaagca     2940

cactaaaaaa aaaaaagatg ttgggggaaa actttggttt cccttcttag taattaaaca     3000

ctctcactct cactctcact ctctccactc agacaaacca accacctggg ctgcagacaa     3060

ccagaaaaaa aaagaacaaa atccagatag aaaaacaaag ggctggacaa ccataaataa     3120

acaatctagg gtctactcca tcttccactg tttcttcttc ttcagactta gctaacaaac     3180

aactcacttc accatggatt acgcaggcat cacgcgtggc tccatcagag gcgaggcctt     3240

gaagaaactc gcagaattga ccatccagaa ccagccatcc agcttgaaag aaatcaacac     3300

cggcatccag aaggacgact ttgccaagtt gttgtctgcc accccgaaaa tccccaccaa     3360

gcacaagttg aacggcaacc acgaattgtc tgaggtcgcc attgccaaaa aggagtacga     3420

ggtgttgatt gccttgagcg acgccacaaa agacccaatc aaagtgacct cccagatcaa     3480

gatcttgatt gacaagttca aggtgtactt gtttgagttg cctgaccaga agttctccta     3540

ctccatcgtg tccaactccg tcaacatcgc cccctggacc ttgctcgggg agaagttgac     3600

cacgggcttg atcaacttgg ccttccagaa caacaagcag cacttggacg aggtcattga     3660

catcttcaac gagttcatcg acaagttctt tggcaacacg gagccgcaat tgaccaactt     3720

cttgaccttg tgcggtgtgt tggacgggtt gattgaccat gccaacttct tgagcgtgtc     3780

ctcgcggacc ttcaagatct tcttgaactt ggactcgtat gtggac                    3826


<210>  3
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Comprises two sequences from the 5' and 3' ends of the CYP52A17 
       cytochrome P450 from Candida tropicalis (i) separated by a 
       sequence comprising a NotI restriction site and a 20 bp stuffer 
       fragment and an XhoI restriciton site and (ii) flanked by XhoI 
       

<400>  3
cgtctcatga ttgaacaact cctagaatat tggtatgtcg ttgtgccagt gttgtacatc       60

atcaaacaac tccttgcata cacaaagact cgcgtcttga tgaaaaagtt gggtgctgct      120

ccagtcacaa acaagttgta cgacaacgct ttcggtatcg tcaatggatg gaaggctctc      180

cagttcaaga aagagggcag ggctcaagag tacaacgatt acaagtttga ccactccaag      240

aacccaagcg tgggcaccta cgtcagtatt cttttcggca ccaggatcgt cgtgaccaaa      300

gatgcggccg ctagatcttg cgaagctcca tctcgagatc aactctactc atttggaccc      360

tgtctattac ggccctgatg ctgctgagtt cagaccagag agatggtttg agccatcaac      420

caaaaagctc ggctgggctt acttgccatt caacggtggt ccaagaatct gtttgggtca      480

gcagtttgcc ttgacggaag ctggctatgt gttggttaga ttggtgcaag agttctccca      540

cgttaggctg gacccagacg aggtgtaccc gccaaagagg ttgaccaact tgaccatgtg      600

tttgcaggat ggtgctattg tcaagtttga ctaggagacg                            640


<210>  4
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A17

<400>  4
cgtctcatga ttgaacaact cctagaatat tggtatgtcg ttgtgccagt gttgtacatc       60

atcaaacaac tccttgcata cacaaagact cgcgtcttga tgaaaaagtt gggtgctgct      120

ccagtcacaa acaagttgta cgacaacgct ttcggtatcg tcaatggatg gaaggctctc      180

cagttcaaga aagagggcag ggctcaagag tacaacgatt acaagtttga ccactccaag      240

aacccaagcg tgggcaccta cgtcagtatt cttttcggca ccaggatcgt cgtgaccaaa      300

gatgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgaga tcaactctac     4500

tcatttggac cctgtctatt acggccctga tgctgctgag ttcagaccag agagatggtt     4560

tgagccatca accaaaaagc tcggctgggc ttacttgcca ttcaacggtg gtccaagaat     4620

ctgtttgggt cagcagtttg ccttgacgga agctggctat gtgttggtta gattggtgca     4680

agagttctcc cacgttaggc tggacccaga cgaggtgtac ccgccaaaga ggttgaccaa     4740

cttgaccatg tgtttgcagg atggtgctat tgtcaagttt gactaggaga cg             4792


<210>  5
<211>  3948
<212>  DNA
<213>  Candida tropicalis

<400>  5
gacctgtgac gcttccggtg tcttgccacc agtctccaag ttgaccgacg cccaagtcat       60

gtaccacttt atttccggtt acacttccaa gatggctggt actgaagaag gtgtcacgga      120

accacaagct actttctccg cttgtttcgg tcaaccattc ttggtgttgc acccaatgaa      180

gtacgctcaa caattgtctg acaagatctc gcaacacaag gctaacgcct ggttgttgaa      240

caccggttgg gttggttctt ctgctgctag aggtggtaag agatgctcat tgaagtacac      300

cagagccatt ttggacgcta tccactctgg tgaattgtcc aaggttgaat acgaaacttt      360

cccagtcttc aacttgaatg tcccaacctc ctgtccaggt gtcccaagtg aaatcttgaa      420

cccaaccaag gcctggaccg gaaggtgttg actccttcaa caaggaaatc aagtctttgg      480

ctggtaagtt tgctgaaaac ttcaagacct atgctgacca agctaccgct gaagtgagag      540

ctgcaggtcc agaagcttaa agatatttat tcattattta gtttgcctat ttatttctca      600

ttacccatca tcattcaaca ctatatataa agttacttcg gatatcattg taatcgtgcg      660

tgtcgcaatt ggatgatttg gaactgcgct tgaaacggat tcatgcacga agcggagata      720

aaagattacg taatttatct cctgagacaa ttttagccgt gttcacacgc ccttctttgt      780

tctgagcgaa ggataaataa ttagacttcc acagctcatt ctaatttccg tcacgcgaat      840

attgaagggg ggtacatgtg gccgctgaat gtgggggcag taaacgcagt ctctcctctc      900

ccaggaatag tgcaacggag gaaggataac ggatagaaag cggaatgcga ggaaaatttt      960

gaacgcgcaa gaaaagcaat atccgggcta ccaggttttg agccagggaa cacactccta     1020

tttctgctca atgactgaac atagaaaaaa caccaagacg caatgaaacg cacatggaca     1080

tttagacctc cccacatgtg atagtttgtc ttaacagaaa agtataataa gaacccatgc     1140

cgtccctttt ctttcgccgc ttcaactttt ttttttttat cttacacaca tcacgaccat     1200

gactgtacac gatattatcg ccacatactt caccaaatgg tacgtgatag taccactcgc     1260

tttgattgct tatagagtcc tcgactactt ctatggcaga tacttgatgt acaagcttgg     1320

tgctaaacca tttttccaga aacagacaga cggctgtttc ggattcaaag ctccgcttga     1380

attgttgaag aagaagagcg acggtaccct catagacttc acactccagc gtatccacga     1440

tctcgatcgt cccgatatcc caactttcac attcccggtc ttttccatca accttgtcaa     1500

tacccttgag ccggagaaca tcaaggccat cttggccact cagttcaacg atttctcctt     1560

gggtaccaga cactcgcact ttgctccttt gttgggtgat ggtatcttta cgttggatgg     1620

cgccggctgg aagcacagca gatctatgtt gagaccacag tttgccagag aacagatttc     1680

ccacgtcaag ttgttggagc cacacgttca ggtgttcttc aaacacgtca gaaaggcaca     1740

gggcaagact tttgacatcc aggaattgtt tttcagattg accgtcgact ccgccaccga     1800

gtttttgttt ggtgaatccg ttgagtcctt gagagatgaa tctatcggca tgtccatcaa     1860

tgcgcttgac tttgacggca aggctggctt tgctgatgct tttaactatt cgcagaatta     1920

tttggcttcg agagcggtta tgcaacaatt gtactgggtg ttgaacggga aaaagtttaa     1980

ggagtgcaac gctaaagtgc acaagtttgc tgactactac gtcaacaagg ctttggactt     2040

gacgcctgaa caattggaaa agcaggatgg ttatgtgttt ttgtacgaat tggtcaagca     2100

aaccagagac aagcaagtgt tgagagacca attgttgaac atcatggttg ctggtagaga     2160

caccaccgcc ggtttgttgt cgtttgtttt ctttgaattg gccagaaacc cagaagttac     2220

caacaagttg agagaagaaa ttgaggacaa gtttggactc ggtgagaatg ctagtgttga     2280

agacatttcc tttgagtcgt tgaagtcctg tgaatacttg aaggctgttc tcaacgaaac     2340

cttgagattg tacccatccg tgccacagaa tttcagagtt gccaccaaga acactaccct     2400

cccaagaggt ggtggtaagg acgggttgtc tcctgttttg gtgagaaagg gtcagaccgt     2460

tatttacggt gtctacgcag cccacagaaa cccagctgtt tacggtaagg acgctcttga     2520

gtttagacca gagagatggt ttgagccaga gacaaagaag cttggctggg ccttcctccc     2580

attcaacggt ggtccaagaa tctgtttggg acagcagttt gccttgacag aagcttcgta     2640

tgtcactgtc aggttgctcc aggagtttgc acacttgtct atggacccag acaccgaata     2700

tccacctaag aaaatgtcgc atttgaccat gtcgcttttc gacggtgcca atattgagat     2760

gtattagagg gtcatgtgtt attttgattg tttagtttgt aattactgat taggttaatt     2820

catggattgt tatttattga taggggtttg cgcgtgttgc attcacttgg gatcgttcca     2880

ggttgatgtt tccttccatc ctgtcgagtc aaaaggagtt ttgttttgta actccggacg     2940

atgttttaaa tagaaggtcg atctccatgt gattgttttg actgttactg tgattatgta     3000

atctgcggac gttatacaag catgtgattg tggttttgca gccttttgca cgacaaatga     3060

tcgtcagacg attacgtaat ctttgttaga ggggtaaaaa aaaacaaaat ggcagccaga     3120

atttcaaaca ttctgcaaac aatgcaaaaa atgggaaact ccaacagaca aaaaaaaaaa     3180

ctccgcagca ctccgaaccc acagaacaat ggggcgccag aattattgac tattgtgact     3240

tttttacgct aacgctcatt gcagtgtagt gcgtcttaca cggggtattg ctttctacaa     3300

tgcaagggca cagttgaagg tttgcaccta acgttgcccc gtgtcaactc aatttgacga     3360

gtaacttcct aagctcgaat tatgcagctc gtgcgtcaac ctatgtgcag gaaagaaaaa     3420

atccaaaaaa atcgaaaatg cgactttcga ttttgaataa accaaaaaga aaaatgtcgc     3480

acttttttct cgctctcgct ctctcgaccc aaatcacaac aaatcctcgc gcgcagtatt     3540

tcgacgaaac cacaacaaat aaaaaaaaca aattctacac cacttctttt tcttcaccag     3600

tcaacaaaaa acaacaaatt atacaccatt tcaacgattt ttgctcttat aaatgctata     3660

taatggttta attcaactca ggtatgttta ttttactgtt ttcagctcaa gtatgttcaa     3720

atactaacta cttttgatgt ttgtcgcttt tctagaatca aaacaacgcc cacaacacgc     3780

cgagcttgtc gaatagacgg tttgtttact cattagatgg tcccagatta cttttcaagc     3840

caaagtctct cgagttttgt ttgctgtttc cccaattcct aactatgaag ggtttttata     3900

aggtccaaag accccaaggc atagtttttt tggttccttc ttgtcgtg                  3948


<210>  6
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis CYP52A13

<400>  6
cgtctcatga ctgtacacga tattatcgcc acatacttca ccaaatggta cgtgatagta       60

ccactcgctt tgattgctta tagagtcctc gactacttct atggcagata cttgatgtac      120

aagcttggtg ctaaaccatt tttccagaaa cagacagacg gctgtttcgg attcaaagct      180

ccgcttgaat tgttgaagaa gaagagcgac ggtaccctca tagacttcac actccagcgt      240

atccacgatc tcgatcgtcc cgatatccca actttcacat tcccggtctt ttccatcaac      300

cttgcggccg ctagatcttg cgaagctcca tctcgaggtc tacgcagccc acagaaaccc      360

agctgtttac ggtaaggacg ctcttgagtt tagaccagag agatggtttg agccagagac      420

aaagaagctt ggctgggcct tcctcccatt caacggtggt ccaagaatct gtttgggaca      480

gcagtttgcc ttgacagaag cttcgtatgt cactgtcagg ttgctccagg agtttgcaca      540

cttgtctatg gacccagaca ccgaatatcc acctaagaaa atgtcgcatt tgaccatgtc      600

gcttttcgac ggtgccaata ttgagatgta ttaggagacg                            640


<210>  7
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A13

<400>  7
cgtctcatga ctgtacacga tattatcgcc acatacttca ccaaatggta cgtgatagta       60

ccactcgctt tgattgctta tagagtcctc gactacttct atggcagata cttgatgtac      120

aagcttggtg ctaaaccatt tttccagaaa cagacagacg gctgtttcgg attcaaagct      180

ccgcttgaat tgttgaagaa gaagagcgac ggtaccctca tagacttcac actccagcgt      240

atccacgatc tcgatcgtcc cgatatccca actttcacat tcccggtctt ttccatcaac      300

cttgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgagg tctacgcagc     4500

ccacagaaac ccagctgttt acggtaagga cgctcttgag tttagaccag agagatggtt     4560

tgagccagag acaaagaagc ttggctgggc cttcctccca ttcaacggtg gtccaagaat     4620

ctgtttggga cagcagtttg ccttgacaga agcttcgtat gtcactgtca ggttgctcca     4680

ggagtttgca cacttgtcta tggacccaga caccgaatat ccacctaaga aaatgtcgca     4740

tttgaccatg tcgcttttcg acggtgccaa tattgagatg tattaggaga cg             4792


<210>  8
<211>  3910
<212>  DNA
<213>  Candida tropicalis

<400>  8
ttacaatcat ggagctcgct aggaacccag atgtctggga gaagctccgc gaagaggtca       60

acacgaactt tggcatggag tcgccagact tgctcacttt tgactctctt agaagctcaa      120

agtacgttca ggcggtgctc aacgaaacgc ttcgtatcta cccgggggtg ccacgaaaca      180

tgaagacagc tacgtgcaac acgacgttgc cgcgtggagg aggcaaagac ggtaaggaac      240

ctattttggt gcagaagggc cagtccgttg ggttgattac tattgccacg cagacggacc      300

cagagtattt tggggcagat gctggtgagt tcaaaccgga gagatggttt gattcaagca      360

tgaagaactt ggggtgtaag tacttgccgt tcaatgctgg gccccggact tgtttggggc      420

agcagtacac tttgattgaa gcgagctatt tgctagtcag gttggcgcag acctaccggg      480

taatcgattt gctgccaggg tcggcgtacc caccaagaaa gaagtcgttg atcaatatga      540

gtgctgccga tggggtggtt gtaaagtttc acaaggatct agatggatat gtaaggtgtg      600

taggaggagc ggagataaat tagatttgat tttgtgtaag gtttagcacg tcaagctact      660

ccgcactttg tgtgtaggga gcacatactc cgtctgcgcc tgtgccaaga gacggcccag      720

gggtagtgtg tggtggtgga agtgcatgtg acacaatacc ctggttctgg ccaattgggg      780

atttagtgta ggtaagctgc gacctgaaac actcctcaac gcttgagaca ctggtgggta      840

gagatgcggg ccaggaggct attcttgtcg tgctacccgt gcacggaaaa tcgattgagg      900

gaagaacaaa tttatccgtg aaatccacag agcggataaa tttgtcacat tgctgcgttg      960

cccacccaca gcattctctt ttctctctct ttgtcttact ccgctcctgt ttccttatcc     1020

agaaatacac accaactcat ataaagatac gctagcccag ctgtctttct ttttcttcac     1080

tttttttggt gtgttgcttt tttggctgct actttctaca accaccacca ccaccaccac     1140

catgattgaa caaatcctag aatattggta tattgttgtg cctgtgttgt acatcatcaa     1200

acaactcatt gcctacagca agactcgcgt cttgatgaaa cagttgggtg ctgctccaat     1260

cacaaaccag ttgtacgaca acgttttcgg tatcgtcaac ggatggaagg ctctccagtt     1320

caagaaagag ggcagagctc aagagtacaa cgatcacaag tttgacagct ccaagaaccc     1380

aagcgtcggc acctatgtca gtattctttt tggcaccaag attgtcgtga ccaaggatcc     1440

agagaatatc aaagctattt tggcaaccca gtttggcgat ttttctttgg gcaagagaca     1500

cgctcttttt aaacctttgt taggtgatgg gatcttcacc ttggacggcg aaggctggaa     1560

gcatagcaga tccatgttaa gaccacagtt tgccagagaa caagttgctc atgtgacgtc     1620

gttggaacca cacttccagt tgttgaagaa gcatatcctt aaacacaagg gtgagtactt     1680

tgatatccag gaattgttct ttagatttac tgtcgactcg gccacggagt tcttatttgg     1740

tgagtccgtg cactccttaa aggacgaaac tatcggtatc aaccaagacg atatagattt     1800

tgctggtaga aaggactttg ctgagtcgtt caacaaagcc caggagtatt tgtctattag     1860

aattttggtg cagaccttct actggttgat caacaacaag gagtttagag actgtaccaa     1920

gctggtgcac aagtttacca actactatgt tcagaaagct ttggatgcta ccccagagga     1980

acttgaaaag caaggcgggt atgtgttctt gtatgagctt gtcaagcaga cgagagaccc     2040

caaggtgttg cgtgaccagt ctttgaacat cttgttggca ggaagagaca ccactgctgg     2100

gttgttgtcc tttgctgtgt ttgagttggc cagaaaccca cacatctggg ccaagttgag     2160

agaggaaatt gaacagcagt ttggtcttgg agaagactct cgtgttgaag agattacctt     2220

tgagagcttg aagagatgtg agtacttgaa agcgttcctt aacgaaacct tgcgtgttta     2280

cccaagtgtc ccaagaaact tcagaatcgc caccaagaat acaacattgc caaggggtgg     2340

tggtccagac ggtacccagc caatcttgat ccaaaaggga gaaggtgtgt cgtatggtat     2400

caactctacc cacttagatc ctgtctatta tggccctgat gctgctgagt tcagaccaga     2460

gagatggttt gagccatcaa ccagaaagct cggctgggct tacttgccat tcaacggtgg     2520

gccacgaatc tgtttgggtc agcagtttgc cttgaccgaa gctggttacg ttttggtcag     2580

attggtgcaa gagttctccc acattaggct ggacccagat gaagtgtatc caccaaagag     2640

gttgaccaac ttgaccatgt gtttgcagga tggtgctatt gtcaagtttg actagtacgt     2700

atgagtgcgt ttgattttgt agtttctgtt tgcagtaatg agataactat tcagataagg     2760

cgggtggatg tacgttttgt aagagtttcc ttacaaccct ggtgggtgtg tgaggttgca     2820

tcttagggag agatagcacc ttttgcagct ctccgtatac agttttactc tttgtaacct     2880

atgccaatca tgtggggatt cattgtttgc ccatggtggt gcatgcaaaa tccccccaac     2940

tacccaatct cacatgaaac tcaagcacac tagaaaaaaa agatgttgcg tgggttcttt     3000

tgatgttggg gaaaactttc gtttcctttc tcagtaatta aacgttctca ctcagacaaa     3060

ccacctgggc tgcagacaac cagaaaaaac aaaatccaga tagaagaaga aagggctgga     3120

caaccataaa taaacaacct agggtccact ccatctttca cttcttcttc ttcagactta     3180

tctaacaaac gactcacttc accatggatt acgcaggtat cacgcgtggg tccatcagag     3240

gcgaagcctt gaagaaactc gccgagttga ccatccagaa ccagccatcc agcttgaaag     3300

aaatcaacac cggcatccag aaggacgact ttgccaagtt gttgtcttcc accccgaaaa     3360

tccacaccaa gcacaagttg aatggcaacc acgaattgtc cgaagtcgcc attgccaaaa     3420

aggagtacga ggtgttgatt gccttgagcg acgccacgaa agaaccaatc aaagtcacct     3480

cccagatcaa gatcttgatt gacaagttca aggtgtactt gtttgagttg cccgaccaga     3540

agttctccta ctccatcgtg tccaactccg ttaacattgc cccctggacc ttgctcggtg     3600

agaagttgac cacgggcttg atcaacttgg cgttccagaa caacaagcag cacttggacg     3660

aagtcatcga catcttcaac gagttcatcg acaagttctt tggcaacaca gagccgcaat     3720

tgaccaactt cttgaccttg tccggtgtgt tggacgggtt gattgaccat gccaacttct     3780

tgagcgtgtc ctccaggacc ttcaagatct tcttgaactt ggactcgttt gtggacaact     3840

cggacttctt gaacgacgtg gagaactact ccgacttttt gtacgacgag ccgaacgagt     3900

accagaactt                                                            3910


<210>  9
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis CYP52A18

<400>  9
cgtctcagag atgcgggcca ggaggctatt cttgtcgtgc tacccgtgca cggaaaatcg       60

attgagggaa gaacaaattt atccgtgaaa tccacagagc ggataaattt gtcacattgc      120

tgcgttgccc acccacagca ttctcttttc tctctctttg tcttactccg ctcctgtttc      180

cttatccaga aatacacacc aactcatata aagatacgct agcccagctg tctttctttt      240

tcttcacttt ttttggtgtg ttgctttttt ggctgctact ttctacaacc accaccacca      300

ccaccaccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctagat      360

cttgcgaagc tccatctcga gtcgtatgag tgcgtttgat tttgtagttt ctgtttgcag      420

taatgagata actattcaga taaggcgggt ggatgtacgt tttgtaagag tttccttaca      480

accctggtgg gtgtgtgagg ttgcatctta gggagagata gcaccttttg cagctctccg      540

tatacagttt tactctttgt aacctatgcc aatcatgtgg ggattcattg tttgcccaag      600

gtggtgcatg caaaatcccc ccaactaccc aatctcacat gaaactcaag cacactagaa      660

aaaaaagatg ttgcgtgggt tgagacg                                          687


<210>  10
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polylinker sequence

<400>  10
ccatggggca atcagtgagt ctcgcaggta ccgcggagct c                           41


<210>  11
<211>  4839
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A18

<400>  11
cgtctcagag atgcgggcca ggaggctatt cttgtcgtgc tacccgtgca cggaaaatcg       60

attgagggaa gaacaaattt atccgtgaaa tccacagagc ggataaattt gtcacattgc      120

tgcgttgccc acccacagca ttctcttttc tctctctttg tcttactccg ctcctgtttc      180

cttatccaga aatacacacc aactcatata aagatacgct agcccagctg tctttctttt      240

tcttcacttt ttttggtgtg ttgctttttt ggctgctact ttctacaacc accaccacca      300

ccaccaccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctctag      360

aactagtgga tctgaagttc ctattctcta gaaagtatag gaacttcctg caggaccacc      420

tttgattgta aatagtaata attaccaccc ttatctaatt atttatttaa cttatttatt      480

tatttattat acatatatac aaatctaata aagtgaaaat ctcccccttc acacttcaca      540

tatgttaggc gtcatcctgt gctcccgaga accagtacca gtacatcgct gtttcgttcg      600

agacttgagg tctagtttta tacgtgaaga ggtcaatgcc gccgagagta aagccacatt      660

ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga      720

gctgtctgct tagtgcccac tttttcgcaa attcgatgag actgtgcgcg actcctttgc      780

ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc catgttgagt      840

tgagttcaat cttcccgaca agctcttggt cgatgaatgc gccatagcaa gcagagtctt      900

catcagagtc atcatccgag atgtaatcct tccggtaggg gctcacactt ctggtagata      960

gttcaaagcc ttggtcggat aggtgcacat cgaacacttc acgaacaatg aaatggttct     1020

cagcatccaa tgtttccgcc acctgctcag ggatcaccga aattttcata tgagaaccgt     1080

tatcgataac taaagcagca acttcttcta taaaaatggg ttagtatgac agtcatttaa     1140

ataaggaatt tttcagttgg cttggtttca attcaatgtt cgtttttttt ttttcttgct     1200

gtgtttgtgt ttgtgttgtt tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata     1260

gtgagccggg aaatctgtat agcccagata acaacacaag tccaaactag aaactcgtca     1320

aacaccaaaa gcaatgttga atcaattgcc ttgcacaagt acacgtagga aaacataaaa     1380

cattgcaatt ttgaatattg agccttttgt cgtaacattg attgatagga ttactcaccg     1440

aatggttttg aaaccactgc cgacagatca atcaatcaat caaaaaacgt gaactttgaa     1500

aaaggggaag aacagataca ttgaagttag ccatttccat tgatcgtcac aacatatctg     1560

ataaattact ttcaaaatta taagctgatg tgtgtgtatt attaatgtga cagtaacatc     1620

ccaaacgaga aatattatgt cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt     1680

tcccacccta cccatttgtc atattgaaac caatcaactg attaatcaat caattagaat     1740

tgaagctaaa ctaaaacata ccaccgtcca ttttgaatga ttatattttt ttaatattaa     1800

tatcgagata atgtttctaa gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag     1860

gaaaggaaaa aaagaaaaat ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa     1920

tcttggtgag aacagcgacc gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa     1980

caactaatta acgtgtgtgt atggatcttt ttttcttttt tctctttaac cgactataaa     2040

caacaaacat ttttgggcag tgcacacact acttaatata cacagcataa attacacgat     2100

tagaaacaaa ttagcttatt aaaataacct aatcaaaccg aatattttat ggtattatga     2160

gtaaactata taatataaat agcacacacc cacaacaaca acaaaggaaa actaaaaggt     2220

tttttctttt tgaaaagatc gttttcttta ttattctcta gttttgacgc tcgacatttt     2280

atgatggaat gaatgggatg aatcatcaaa caagagaaaa tacccgtgac gaaaataata     2340

aaataagttc ctctgataca gaagatgaaa acaacaacaa caagatatag aaatgccttg     2400

ggtggctatt ttatagtctt aactttttaa tgtatatttg ttttgttttt ttacataata     2460

atactttata aaagctaagc taaattcaag taaaatttca atctctcaaa taaaacattt     2520

ttctcttttt cttaaattta gttttatata tttataaaat atacaaagat ttttttaaaa     2580

aagtaacaag ttatatatgt aataacaaaa agaagaataa caagaataca aaaccagatt     2640

tccagatttc cagaatttca ctcttatatg cgtctattta tgtaggatga aaggtagtct     2700

agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta     2760

ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct     2820

atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga     2880

tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta     2940

tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag     3000

gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa     3060

ggcgcatttt tcttcaaagc tttattgtac gatctgacta agttatcttt taataattgg     3120

tattcctgtt tattgcttga agaattgccg gtcctattta ctcgttttag gactggttca     3180

gaattcctca aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag     3240

aagtatatgt gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact     3300

cccaaatact tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg     3360

aatcttccac aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt     3420

gtaaatctcg aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt     3480

tcaccctcac ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct     3540

tcttccgatg attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga     3600

tgtttttgtc catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat     3660

gaggcttcca aaattgttgc tttttgcgtc ttgtatttaa actggagtga tttattgaca     3720

atatcgaaac tcaacgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg     3780

attgctgttc cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat     3840

gctattttct cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta     3900

ggtggtgttt tacataatat atcaaattgt ggcatgtcga cgattattag ttaaaccact     3960

gcaaaaagtt ggggaaaatt ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc     4020

cactccccaa tctttgaatt attccgaaat attcagcgaa cggggtgtac acaaaaacta     4080

acattctcaa ctgcataatt tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa     4140

ccatagcaat catggaatac ggtaaatttg tgttgttcgg tgactccatc acccagttta     4200

gttgtaccca gtatggcttt catccagcat tacagaatgt gtatatccga aaattggatg     4260

ttattaaccg tggtttcagt ggctacaact cagagcacgc tagacaaatt cttccaaaaa     4320

ttttagagtc ggaaaccaat atcaaattga tgacaatatt ttttggaact aacgatgcat     4380

acgactacat caatgaaatc cagacagtcg agttagacag atataaagat aatttaagtg     4440

taatggtaca gatggtacta gacaaaaata tcaaaccaat cattattgga tccgaagttc     4500

ctattctcta gaaagtatag gaacttcctc gagtcgtatg agtgcgtttg attttgtagt     4560

ttctgtttgc agtaatgaga taactattca gataaggcgg gtggatgtac gttttgtaag     4620

agtttcctta caaccctggt gggtgtgtga ggttgcatct tagggagaga tagcaccttt     4680

tgcagctctc cgtatacagt tttactcttt gtaacctatg ccaatcatgt ggggattcat     4740

tgtttgccca aggtggtgca tgcaaaatcc ccccaactac ccaatctcac atgaaactca     4800

agcacactag aaaaaaaaga tgttgcgtgg gttgagacg                            4839


<210>  12
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NotI-XhoI and 20 base pair stuffer

<400>  12
gcggccgcta gatcttgcga agctccatct cgag                                   34


<210>  13
<211>  3755
<212>  DNA
<213>  Candida tropicalis

<400>  13
gctcaacaat tgtctgacaa gatctcgcaa cacaaggcta acgcctggtt gttgaacact       60

ggttgggttg gttcttctgc tgctagaggt ggtaagagat gttcattgaa gtacaccaga      120

gccattttgg acgctatcca ctctggtgaa ttgtccaagg ttgaatacga gactttccca      180

gtcttcaact tgaatgtccc aacctcctgc ccaggtgtcc caagtgaaat cttgaaccca      240

accaaggcct ggaccgaagg tgttgactcc ttcaacaagg aaatcaagtc tttggctggt      300

aagtttgctg aaaacttcaa gacctatgct gaccaagcta ccgctgaagt tagagctgca      360

ggtccagaag cttaaagata tttattcact atttagtttg cctatttatt tctcatcacc      420

catcatcatt caacaatata tataaagtta tttcggaact catatatcat tgtaatcgtg      480

cgtgttgcaa ttgggtaatt tgaaactgta gttggaacgg attcatgcac gatgcggaga      540

taacacgaga ttatctccta agacaatttt ggcctcattc acacgccctt cttctgagct      600

aaggataaat aattagactt cacaagttca ttaaaatatc cgtcacgcga aaactgcaac      660

aataaggaag gggggggtag acgtagccga tgaatgtggg gtgccagtaa acgcagtctc      720

tctctccccc cccccccccc ccccctcagg aatagtacaa cgggggaagg ataacggata      780

gcaagtggaa tgcgaggaaa attttgaatg cgcaaggaaa gcaatatccg ggctatcagg      840

ttttgagcca ggggacacac tcctcttctg cacaaaaact taacgtagac aaaaaaaaaa      900

aactccacca agacacaatg aatcgcacat ggacatttag acctccccac atgtgaaagc      960

ttctctggcg aaagcaaaaa aagtataata aggacccatg ccttccctct tcctgggccg     1020

tttcaacttt ttctttttct ttgtctatca acacacacac acctcacgac catgactgca     1080

caggatatta tcgccacata catcaccaaa tggtacgtga tagtaccact cgctttgatt     1140

gcttataggg tcctcgacta cttttacggc agatacttga tgtacaagct tggtgctaaa     1200

ccgtttttcc agaaacaaac agacggttat ttcggattca aagctccact tgaattgtta     1260

aaaaagaaga gtgacggtac cctcatagac ttcactctcg agcgtatcca agcgctcaat     1320

cgtccagata tcccaacttt tacattccca atcttttcca tcaaccttat cagcaccctt     1380

gagccggaga acatcaaggc tatcttggcc acccagttca acgatttctc cttgggcacc     1440

agacactcgc actttgctcc tttgttgggc gatggtatct ttaccttgga cggtgccggc     1500

tggaagcaca gcagatctat gttgagacca cagtttgcca gagaacagat ttcccacgtc     1560

aagttgttgg agccacacat gcaggtgttc ttcaagcacg tcagaaaggc acagggcaag     1620

acttttgaca tccaagaatt gtttttcaga ttgaccgtcg actccgccac tgagtttttg     1680

tttggtgaat ccgttgagtc cttgagagat gaatctattg ggatgtccat caatgcactt     1740

gactttgacg gcaaggctgg ctttgctgat gcttttaact actcgcagaa ctatttggct     1800

tcgagagcgg ttatgcaaca attgtactgg gtgttgaacg ggaaaaagtt taaggagtgc     1860

aacgctaaag tgcacaagtt tgctgactat tacgtcagca aggctttgga cttgacacct     1920

gaacaattgg aaaagcagga tggttatgtg ttcttgtacg agttggtcaa gcaaaccaga     1980

gacaggcaag tgttgagaga ccagttgttg aacatcatgg ttgccggtag agacaccacc     2040

gccggtttgt tgtcgtttgt tttctttgaa ttggccagaa acccagaggt gaccaacaag     2100

ttgagagaag aaatcgagga caagtttggt cttggtgaga atgctcgtgt tgaagacatt     2160

tcctttgagt cgttgaagtc atgtgaatac ttgaaggctg ttctcaacga aactttgaga     2220

ttgtacccat ccgtgccaca gaatttcaga gttgccacca aaaacactac ccttccaagg     2280

ggaggtggta aggacgggtt atctcctgtt ttggtcagaa agggtcaaac cgttatgtac     2340

ggtgtctacg ctgcccacag aaacccagct gtctacggta aggacgccct tgagtttaga     2400

ccagagaggt ggtttgagcc agagacaaag aagcttggct gggccttcct tccattcaac     2460

ggtggtccaa gaatttgctt gggacagcag tttgccttga cagaagcttc gtatgtcact     2520

gtcagattgc tccaagagtt tggacacttg tctatggacc ccaacaccga atatccacct     2580

aggaaaatgt cgcatttgac catgtccctt ttcgacggtg ccaacattga gatgtattag     2640

aggatcatgt gttatttttg attggtttag tctgtttgta gctattgatt aggttaattc     2700

acggattgtt atttattgat agggggtgcg tgtgtgtgtg tgtgttgcat tcacatggga     2760

tcgttccagg ttgttgtttc cttccatcct gttgagtcaa aaggagtttt gttttgtaac     2820

tccggacgat gtcttagata gaaggtcgat ctccatgtga ttgtttgact gctactctga     2880

ttatgtaatc tgtaaagcct agacgttatg caagcatgtg attgtggttt ttgcaacctg     2940

tttgcacgac aaatgatcga cagtcgatta cgtaatccat attatttaga ggggtaataa     3000

aaaataaatg gcagccagaa tttcaaacat tttgcaaaca atgcaaaaga tgagaaactc     3060

caacagaaaa aataaaaaaa ctccgcagca ctccgaacca acaaaacaat ggggggcgcc     3120

agaattattg actattgtga ctttttttta ttttttccgt taactttcat tgcagtgaag     3180

tgtgttacac ggggtggtga tggtgttggt ttctacaatg caagggcaca gttgaaggtt     3240

tccacataac gttgcaccat atcaactcaa tttatcctca ttcatgtgat aaaagaagag     3300

ccaaaaggta attggcagac cccccaaggg gaacacggag tagaaagcaa tggaaacacg     3360

cccatgacag tgccatttag cccacaacac atctagtatt cttttttttt tttgtgcgca     3420

ggtgcacacc tggactttag ttattgcccc ataaagttaa caatctcacc tttggctctc     3480

ccagtgtctc cgcctccaga tgctcgtttt acaccctcga gctaacgaca acacaacacc     3540

catgagggga atgggcaaag ttaaacactt ttggtttcaa tgattcctat ttgctactct     3600

cttgttttgt gttttgattt gcaccatgtg aaataaacga caattatata taccttttcg     3660

tctgtcctcc aatgtctctt tttgctgcca ttttgctttt tgctttttgc ttttgcactc     3720

tctcccactc ccacaatcag tgcagcaaca cacaa                                3755


<210>  14
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis CYP52A14

<400>  14
cgtctcgata acggatagca agtggaatgc gaggaaaatt ttgaatgcgc aaggaaagca       60

atatccgggc tatcaggttt tgagccaggg gacacactcc tcttctgcac aaaaacttaa      120

cgtagacaaa aaaaaaaaac tccaccaaga cacaatgaat cgcacatgga catttagacc      180

tccccacatg tgaaagcttc tctggcgaaa gcaaaaaaag tataataagg acccatgcct      240

tccctcttcc tgggccgttt caactttttc tttttctttg tctatcaaca cacacacacc      300

tcacgaccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctagat      360

cttgcgaagc tccatctcga gaggatcatg tgttattttt gattggttta gtctgtttgt      420

agctattgat taggttaatt cacggattgt tatttattga tagggggtgc gtgtgtgtgt      480

gtgtgttgca ttcacatggg atcgttccag gttgttgttt ccttccatcc tgttgagtca      540

aaaggagttt tgttttgtaa ctccggacga tgtcttagat agaaggtcga tctccatgtg      600

attgtttgac tgctactctg attatgtaat ctgtaaagcc tagacgttat gcaagcatgt      660

gattgtggtt tttgcaacct ggagacg                                          687


<210>  15
<211>  4839
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A14

<400>  15
cgtctcgata acggatagca agtggaatgc gaggaaaatt ttgaatgcgc aaggaaagca       60

atatccgggc tatcaggttt tgagccaggg gacacactcc tcttctgcac aaaaacttaa      120

cgtagacaaa aaaaaaaaac tccaccaaga cacaatgaat cgcacatgga catttagacc      180

tccccacatg tgaaagcttc tctggcgaaa gcaaaaaaag tataataagg acccatgcct      240

tccctcttcc tgggccgttt caactttttc tttttctttg tctatcaaca cacacacacc      300

tcacgaccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctctag      360

aactagtgga tctgaagttc ctattctcta gaaagtatag gaacttcctg caggaccacc      420

tttgattgta aatagtaata attaccaccc ttatctaatt atttatttaa cttatttatt      480

tatttattat acatatatac aaatctaata aagtgaaaat ctcccccttc acacttcaca      540

tatgttaggc gtcatcctgt gctcccgaga accagtacca gtacatcgct gtttcgttcg      600

agacttgagg tctagtttta tacgtgaaga ggtcaatgcc gccgagagta aagccacatt      660

ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga      720

gctgtctgct tagtgcccac tttttcgcaa attcgatgag actgtgcgcg actcctttgc      780

ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc catgttgagt      840

tgagttcaat cttcccgaca agctcttggt cgatgaatgc gccatagcaa gcagagtctt      900

catcagagtc atcatccgag atgtaatcct tccggtaggg gctcacactt ctggtagata      960

gttcaaagcc ttggtcggat aggtgcacat cgaacacttc acgaacaatg aaatggttct     1020

cagcatccaa tgtttccgcc acctgctcag ggatcaccga aattttcata tgagaaccgt     1080

tatcgataac taaagcagca acttcttcta taaaaatggg ttagtatgac agtcatttaa     1140

ataaggaatt tttcagttgg cttggtttca attcaatgtt cgtttttttt ttttcttgct     1200

gtgtttgtgt ttgtgttgtt tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata     1260

gtgagccggg aaatctgtat agcccagata acaacacaag tccaaactag aaactcgtca     1320

aacaccaaaa gcaatgttga atcaattgcc ttgcacaagt acacgtagga aaacataaaa     1380

cattgcaatt ttgaatattg agccttttgt cgtaacattg attgatagga ttactcaccg     1440

aatggttttg aaaccactgc cgacagatca atcaatcaat caaaaaacgt gaactttgaa     1500

aaaggggaag aacagataca ttgaagttag ccatttccat tgatcgtcac aacatatctg     1560

ataaattact ttcaaaatta taagctgatg tgtgtgtatt attaatgtga cagtaacatc     1620

ccaaacgaga aatattatgt cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt     1680

tcccacccta cccatttgtc atattgaaac caatcaactg attaatcaat caattagaat     1740

tgaagctaaa ctaaaacata ccaccgtcca ttttgaatga ttatattttt ttaatattaa     1800

tatcgagata atgtttctaa gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag     1860

gaaaggaaaa aaagaaaaat ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa     1920

tcttggtgag aacagcgacc gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa     1980

caactaatta acgtgtgtgt atggatcttt ttttcttttt tctctttaac cgactataaa     2040

caacaaacat ttttgggcag tgcacacact acttaatata cacagcataa attacacgat     2100

tagaaacaaa ttagcttatt aaaataacct aatcaaaccg aatattttat ggtattatga     2160

gtaaactata taatataaat agcacacacc cacaacaaca acaaaggaaa actaaaaggt     2220

tttttctttt tgaaaagatc gttttcttta ttattctcta gttttgacgc tcgacatttt     2280

atgatggaat gaatgggatg aatcatcaaa caagagaaaa tacccgtgac gaaaataata     2340

aaataagttc ctctgataca gaagatgaaa acaacaacaa caagatatag aaatgccttg     2400

ggtggctatt ttatagtctt aactttttaa tgtatatttg ttttgttttt ttacataata     2460

atactttata aaagctaagc taaattcaag taaaatttca atctctcaaa taaaacattt     2520

ttctcttttt cttaaattta gttttatata tttataaaat atacaaagat ttttttaaaa     2580

aagtaacaag ttatatatgt aataacaaaa agaagaataa caagaataca aaaccagatt     2640

tccagatttc cagaatttca ctcttatatg cgtctattta tgtaggatga aaggtagtct     2700

agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta     2760

ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct     2820

atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga     2880

tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta     2940

tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag     3000

gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa     3060

ggcgcatttt tcttcaaagc tttattgtac gatctgacta agttatcttt taataattgg     3120

tattcctgtt tattgcttga agaattgccg gtcctattta ctcgttttag gactggttca     3180

gaattcctca aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag     3240

aagtatatgt gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact     3300

cccaaatact tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg     3360

aatcttccac aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt     3420

gtaaatctcg aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt     3480

tcaccctcac ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct     3540

tcttccgatg attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga     3600

tgtttttgtc catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat     3660

gaggcttcca aaattgttgc tttttgcgtc ttgtatttaa actggagtga tttattgaca     3720

atatcgaaac tcaacgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg     3780

attgctgttc cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat     3840

gctattttct cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta     3900

ggtggtgttt tacataatat atcaaattgt ggcatgtcga cgattattag ttaaaccact     3960

gcaaaaagtt ggggaaaatt ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc     4020

cactccccaa tctttgaatt attccgaaat attcagcgaa cggggtgtac acaaaaacta     4080

acattctcaa ctgcataatt tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa     4140

ccatagcaat catggaatac ggtaaatttg tgttgttcgg tgactccatc acccagttta     4200

gttgtaccca gtatggcttt catccagcat tacagaatgt gtatatccga aaattggatg     4260

ttattaaccg tggtttcagt ggctacaact cagagcacgc tagacaaatt cttccaaaaa     4320

ttttagagtc ggaaaccaat atcaaattga tgacaatatt ttttggaact aacgatgcat     4380

acgactacat caatgaaatc cagacagtcg agttagacag atataaagat aatttaagtg     4440

taatggtaca gatggtacta gacaaaaata tcaaaccaat cattattgga tccgaagttc     4500

ctattctcta gaaagtatag gaacttcctc gagaggatca tgtgttattt ttgattggtt     4560

tagtctgttt gtagctattg attaggttaa ttcacggatt gttatttatt gatagggggt     4620

gcgtgtgtgt gtgtgtgttg cattcacatg ggatcgttcc aggttgttgt ttccttccat     4680

cctgttgagt caaaaggagt tttgttttgt aactccggac gatgtcttag atagaaggtc     4740

gatctccatg tgattgtttg actgctactc tgattatgta atctgtaaag cctagacgtt     4800

atgcaagcat gtgattgtgg tttttgcaac ctggagacg                            4839


<210>  16
<211>  4296
<212>  DNA
<213>  Candida tropicalis

<400>  16
tgcatactcg gagcatatcg ccatcgtcca tatcgttggc actccatcca ctgagtcagc       60

caagaagcaa ttgttgttgc accacacctt aggtaatggt gactttactg ttttccacaa      120

gatctcgtca ttcatcagtg ccactactgc tgggttgacc gacccagaca ccgccgctga      180

tgaaattgat agagtgattg agtcagccta catcaaccag cgtccaacgt acttgggatt      240

cccttccaac atggttgacg ttcaagtgcc agtcagcaag ttggacaagc cattgaactt      300

aaccccacct gcaaacaatc caaagatcca gtctgaggtc ttgagcgaca ttattgcctt      360

gattgaaacc gccaaggatc cagttatcat cattgatgct tgttgtggaa ggcacaatgc      420

taccccagag gcacagaagt tgattgagtt gacaaagttc aagtttgctg tcaccccaat      480

ggctaaaggg tctaaggaca ttgatgaaag tgatccgaag ttcattggtt gctacgttgg      540

tgacttgtct tatccaagag tcaaagagtt ggttgaaagc tcggacttgg tcttgtcctt      600

gggtgctgtc ttgtctgatt tcaacactgg ttcgttctca tactctttgg acaatgccaa      660

ggttgttgaa ttccactccg actacactca aatcaagagc gctcagtacc caggtatcag      720

aatgaaggaa ttgttgggca agttggttga ggagccagaa ttggtcaaga cgtgttccaa      780

gatcccagca aagaagttgg tcactgacaa ctttgaacca ttggtcttgc caccggacca      840

caagctcacc caatcctggt tgtggagtaa cttgggtaat tggttgaaag aaggtgatgt      900

gattgttacc gaaaccggta cttccaattt cggtattgtc cagaccaaat tcccaaagaa      960

tgctgtcggt atctcgcaag tcttgtgggg ttccattggc tactcggtcg gttctgccgc     1020

tggtgccgtt atcgccgccg aggagcttga tcccagccgt agagtcatct tgtttgttgg     1080

tgacggttct ttgcagttga ccgtgcagga aatctccacc atggccagac acaagaacaa     1140

catctacatc tttgtcttga acaacaacgg tttcaccatt gaaagattga ttcacggtcc     1200

agaagctggt tacaacagta ttcaagaatg ggagaacgct gagttattga agactttcaa     1260

ggctaccaac tacgagagtt tcaccgtcaa gactgtcggc gaacttgaca aggtgttcaa     1320

ggatgaaaag tttgccgtca acgacaagat tagattggtt gagatcatgt tagacacttt     1380

cgatgctcca gagaacttgg ttaagcaagc tgagagatct gccaacacca acaagtagag     1440

tttgtctatg ttttccgttt gccttttctt tctagtacga gacgttattg aacgaagttt     1500

ttatatatct agatctaata catattccat gtctgttcat ttttgacgga gtttcataag     1560

gtggcagttt ctaatcaaag gtccgtcatt ggcgtcgtgg cattggcggc tcgcatcaac     1620

tcgtatgtca atattttctg ttaactccgc cagacatacg atcaaaacct acaagcaaaa     1680

aaattccaca tgctttgttt gagatctcca caaacaacaa cggggtaaga aaatcatggg     1740

gcgattaatc atgccatctt tgtaaatttc tttgtttcaa catcaccctc tttagtcaaa     1800

ccttcacagg actgtctgct ctactttgcc acccagttca tatataaatt accaacttcc     1860

accgagcacc accaacacct caccccactc tctccccccc cctttttttt ccagcttaga     1920

cacacacttc aaactcgaca tggctccatt tttgcccgac caggtcgact acaaacacgt     1980

cgacaccctt atgttattat gtgacgggat catccacgaa accaccgtgg acgaaatcaa     2040

agacgtcatt gcccctgact tccccgccga caaatacgag gagtacgtca ggacattcac     2100

caaaccctcc gaaaccccag ggttcaggga aaccgtctac aacaccgtca acgcaaacac     2160

catggatgca atccaccagt tcattatctt gaccaatgtt ttgggatcaa gggtcttggc     2220

accagctttg accaactcgt tgactcctat caaggacatg agcttggaag accgtgaaaa     2280

gttgttagcc tcgtggcgtg actcccctat tgctgctaaa aggaagttgt tcaggttggt     2340

ttctacgctt accttggtca cgttcacgag attggccaat gagttgcatt tgaaagccat     2400

tcattatcca ggaagagaag accgtgaaaa ggcttatgaa acccaggaga ttgacccttt     2460

taagtaccag tttttggaaa aaccgaagtt ttacggcgct gagttgtact tgccagatat     2520

tgatgtgatc attattggat ctggggccgg tgctggtgtc gtggcccaca ctttgaccaa     2580

cgacggcttc aagagtttgg ttttggaaaa gggcagatac tttagcaact ccgagttgaa     2640

ctttgatgac aaggacgggg ttcaagaatt ataccaaagt ggaggtactt tgaccaccgt     2700

caaccagcag ttgtttgttc ttgctggttc cacttttggt ggtggtacca ctgtcaattg     2760

gtcggcctgt cttaaaacgc cattcaaggt gcgtaaggaa tggtatgatg agtttggcgt     2820

tgactttgct gccgatgaag cctacgacaa agcacaggat tatgtttggc agcaaatggg     2880

agcttctacc gaaggcatca cccactcttt ggctaacgag attattattg aaggtggcaa     2940

gaaattaggt tacaaggcca aggtattaga ccaaaacagc ggtggtcatc ctcatcacag     3000

atgcggtttc tgttatttgg gttgtaagca cggtatcaag cagggctctg ttaataactg     3060

gtttagagac gcagctgccc acggttctca gttcatgcaa caggttagag ttttgcaaat     3120

ccttaacaag aagggcatcg cttatggtat cttgtgtgag gatgttgtaa ccggtgccaa     3180

gttcaccatt actggcccca aaaagtttgt tgttgccgcc ggcgccttaa acactccatc     3240

tgtgttggtc aactccggat tcaagaacaa gaacatcggt aagaacttaa ctttgcatcc     3300

agtttctgtc gtgtttggtg attttggcaa agacgttcaa gcagatcact tccacaactc     3360

catcatgact gctctttgtt cagaagccgc tgatttagac ggcaagggtc atggatgcag     3420

aattgaaacc atcttgaacg ctccattcat ccaggcttca ttcttaccat ggagaggtag     3480

taacgaggct agacgagact tgttgcgtta caacaacatg gtggccatgt tacttcttag     3540

tcgtgatacc accagtggtt ccgtttcgtc ccatccaact aaacctgaag cattagttgt     3600

cgagtacgac gtgaacaagt ttgacagaaa ctccatcttg caggcattgt tggtcactgc     3660

tgacttgttg tacattcaag gtgccaagag aatccttagt ccccaaccat gggtgccaat     3720

ttttgaatcc gacaagccaa aggataagag atcaatcaag gacgaggact atgtcgaatg     3780

gagagccaag gttgccaaga ttccttttga cacctacggc tcgccttatg gttcggcgca     3840

tcaaatgtct tcttgtcgta tgtcaggtaa gggtcctaaa tacggtgctg ttgataccga     3900

tggtagattg tttgaatgtt cgaatgttta tgttgctgac gctagtcttt tgccaactgc     3960

tagcggtgct aatcctatgg tcaccaccat gactcttgca agacatgttg cgttaggttt     4020

ggcagactcc ttgaagacca aggccaagtt gtagttctgt atacgtatct tataatttag     4080

atttcctttt attgacggta aacattcagg ataggtacta cccttgctgc aaaagcccag     4140

cacgccccaa tcgcgatgac ttgagcgaag caaacacgca cacaaaaggg gtacacaaaa     4200

aataacgaga tgcccttgaa gcacacaccc aaacacgatg gaacacaaga tggccctaga     4260

aagtacaaaa aaagtaaagc cacttgattc cgccca                               4296


<210>  17
<211>  609
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis FAO1

<400>  17
cgtctcctgt taactccgcc agacatacga tcaaaaccta caagcaaaaa aattccacat       60

gctttgtttg agatctccac aaacaacaac ggggtaagaa aatcatgggg cgattaatca      120

tgccatcttt gtaaatttct ttgtttcaac atcaccctct ttagtcaaac cttcacagga      180

ctgtctgctc tactttgcca cccagttcat atataaatta ccaacttcca ccgagcacca      240

ccaacacctc accccactct ctcccccccc cttttttttc cagcttagac acacacttca      300

aactcgccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctagat      360

cttgcgaagc tccatctcga gttctgtata cgtatcttat aatttagatt tccttttatt      420

gacggtaaac attcaggata ggtactaccc ttgctgcaaa agcccagcac gccccaatcg      480

cgatgacttg agcgaagcaa acacgcacac aaaaggggta cacaaaaaat aacgagatgc      540

ccttgaagca cacacccaaa cacgatggaa cacaagatgg ccctagaaag tacaaaaaaa      600

gtagagacg                                                              609


<210>  18
<211>  4761
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis FAO1

<400>  18
cgtctcctgt taactccgcc agacatacga tcaaaaccta caagcaaaaa aattccacat       60

gctttgtttg agatctccac aaacaacaac ggggtaagaa aatcatgggg cgattaatca      120

tgccatcttt gtaaatttct ttgtttcaac atcaccctct ttagtcaaac cttcacagga      180

ctgtctgctc tactttgcca cccagttcat atataaatta ccaacttcca ccgagcacca      240

ccaacacctc accccactct ctcccccccc cttttttttc cagcttagac acacacttca      300

aactcgccat ggggcaatca gtgagtctcg caggtaccgc ggagctcgcg gccgctctag      360

aactagtgga tctgaagttc ctattctcta gaaagtatag gaacttcctg caggaccacc      420

tttgattgta aatagtaata attaccaccc ttatctaatt atttatttaa cttatttatt      480

tatttattat acatatatac aaatctaata aagtgaaaat ctcccccttc acacttcaca      540

tatgttaggc gtcatcctgt gctcccgaga accagtacca gtacatcgct gtttcgttcg      600

agacttgagg tctagtttta tacgtgaaga ggtcaatgcc gccgagagta aagccacatt      660

ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga      720

gctgtctgct tagtgcccac tttttcgcaa attcgatgag actgtgcgcg actcctttgc      780

ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc catgttgagt      840

tgagttcaat cttcccgaca agctcttggt cgatgaatgc gccatagcaa gcagagtctt      900

catcagagtc atcatccgag atgtaatcct tccggtaggg gctcacactt ctggtagata      960

gttcaaagcc ttggtcggat aggtgcacat cgaacacttc acgaacaatg aaatggttct     1020

cagcatccaa tgtttccgcc acctgctcag ggatcaccga aattttcata tgagaaccgt     1080

tatcgataac taaagcagca acttcttcta taaaaatggg ttagtatgac agtcatttaa     1140

ataaggaatt tttcagttgg cttggtttca attcaatgtt cgtttttttt ttttcttgct     1200

gtgtttgtgt ttgtgttgtt tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata     1260

gtgagccggg aaatctgtat agcccagata acaacacaag tccaaactag aaactcgtca     1320

aacaccaaaa gcaatgttga atcaattgcc ttgcacaagt acacgtagga aaacataaaa     1380

cattgcaatt ttgaatattg agccttttgt cgtaacattg attgatagga ttactcaccg     1440

aatggttttg aaaccactgc cgacagatca atcaatcaat caaaaaacgt gaactttgaa     1500

aaaggggaag aacagataca ttgaagttag ccatttccat tgatcgtcac aacatatctg     1560

ataaattact ttcaaaatta taagctgatg tgtgtgtatt attaatgtga cagtaacatc     1620

ccaaacgaga aatattatgt cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt     1680

tcccacccta cccatttgtc atattgaaac caatcaactg attaatcaat caattagaat     1740

tgaagctaaa ctaaaacata ccaccgtcca ttttgaatga ttatattttt ttaatattaa     1800

tatcgagata atgtttctaa gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag     1860

gaaaggaaaa aaagaaaaat ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa     1920

tcttggtgag aacagcgacc gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa     1980

caactaatta acgtgtgtgt atggatcttt ttttcttttt tctctttaac cgactataaa     2040

caacaaacat ttttgggcag tgcacacact acttaatata cacagcataa attacacgat     2100

tagaaacaaa ttagcttatt aaaataacct aatcaaaccg aatattttat ggtattatga     2160

gtaaactata taatataaat agcacacacc cacaacaaca acaaaggaaa actaaaaggt     2220

tttttctttt tgaaaagatc gttttcttta ttattctcta gttttgacgc tcgacatttt     2280

atgatggaat gaatgggatg aatcatcaaa caagagaaaa tacccgtgac gaaaataata     2340

aaataagttc ctctgataca gaagatgaaa acaacaacaa caagatatag aaatgccttg     2400

ggtggctatt ttatagtctt aactttttaa tgtatatttg ttttgttttt ttacataata     2460

atactttata aaagctaagc taaattcaag taaaatttca atctctcaaa taaaacattt     2520

ttctcttttt cttaaattta gttttatata tttataaaat atacaaagat ttttttaaaa     2580

aagtaacaag ttatatatgt aataacaaaa agaagaataa caagaataca aaaccagatt     2640

tccagatttc cagaatttca ctcttatatg cgtctattta tgtaggatga aaggtagtct     2700

agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta     2760

ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct     2820

atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga     2880

tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta     2940

tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag     3000

gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa     3060

ggcgcatttt tcttcaaagc tttattgtac gatctgacta agttatcttt taataattgg     3120

tattcctgtt tattgcttga agaattgccg gtcctattta ctcgttttag gactggttca     3180

gaattcctca aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag     3240

aagtatatgt gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact     3300

cccaaatact tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg     3360

aatcttccac aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt     3420

gtaaatctcg aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt     3480

tcaccctcac ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct     3540

tcttccgatg attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga     3600

tgtttttgtc catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat     3660

gaggcttcca aaattgttgc tttttgcgtc ttgtatttaa actggagtga tttattgaca     3720

atatcgaaac tcaacgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg     3780

attgctgttc cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat     3840

gctattttct cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta     3900

ggtggtgttt tacataatat atcaaattgt ggcatgtcga cgattattag ttaaaccact     3960

gcaaaaagtt ggggaaaatt ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc     4020

cactccccaa tctttgaatt attccgaaat attcagcgaa cggggtgtac acaaaaacta     4080

acattctcaa ctgcataatt tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa     4140

ccatagcaat catggaatac ggtaaatttg tgttgttcgg tgactccatc acccagttta     4200

gttgtaccca gtatggcttt catccagcat tacagaatgt gtatatccga aaattggatg     4260

ttattaaccg tggtttcagt ggctacaact cagagcacgc tagacaaatt cttccaaaaa     4320

ttttagagtc ggaaaccaat atcaaattga tgacaatatt ttttggaact aacgatgcat     4380

acgactacat caatgaaatc cagacagtcg agttagacag atataaagat aatttaagtg     4440

taatggtaca gatggtacta gacaaaaata tcaaaccaat cattattgga tccgaagttc     4500

ctattctcta gaaagtatag gaacttcctc gagttctgta tacgtatctt ataatttaga     4560

tttcctttta ttgacggtaa acattcagga taggtactac ccttgctgca aaagcccagc     4620

acgccccaat cgcgatgact tgagcgaagc aaacacgcac acaaaagggg tacacaaaaa     4680

ataacgagat gcccttgaag cacacaccca aacacgatgg aacacaagat ggccctagaa     4740

agtacaaaaa aagtagagac g                                               4761


<210>  19
<211>  1992
<212>  DNA
<213>  Candida tropicalis

<400>  19
cttatgttat tatgtgacgg gatcatccac gaaaccaccg tcgaccaaat caaagacgtt       60

attgctcctg acttccctgc tgacaagtac gaagagtacg tcaggacatt caccaaaccc      120

tccgaaaccc cagggttcag ggaaaccgtc tacaacacag tcaacgcaaa caccacggac      180

gcaatccacc agttcattat cttgaccaat gttttggcat ccagggtctt ggctccagct      240

ttgaccaact cgttgacgcc tatcaaggac atgagcttgg aagaccgtga aaaattgttg      300

gcctcgtggc gcgactcccc aatcgctgcc aaaaggaaat tgttcaggtt ggtttccacg      360

cttaccttgg ttactttcac gagattggcc aatgagttgc atttgaaagc cattcactat      420

ccaggaagag aagaccgtga aaaggcttat gaaacccagg agattgaccc tttcaagtac      480

cagtttatgg aaaagccaaa gtttgacggc gctgagttgt acttgccaga tattgatgtt      540

atcattattg gatctggtgc cggtgctggt gttgtggccc acactttggc caacgatggc      600

ttcaagagtt tggttttgga aaagggcaaa tactttagca actccgagtt gaactttgat      660

gacaaggacg gcgttcaaga attataccaa agtggaggta ctttgactac agtcaaccaa      720

cagttgtttg ttcttgctgg ttccactttt ggtggcggta ccactgtcaa ttggtcagcc      780

tgtcttaaga cgccattcaa ggtgcgtaag gaatggtatg atgagtttgg tgttgacttt      840

gctgctgatg aagcatacga taaagcgcag gattatgttt ggcagcaaat gggagcttct      900

accgaaggca tcacccactc tttggctaac gagattatta ttgaaggtgg taagaaatta      960

ggttacaagg ccaaggtatt agaccaaaac agcggtggtc atcctcagca cagatgcggt     1020

ttctgttatt tgggctgtaa gcacggtatc aagcagggtt ctgttaataa ctggtttaga     1080

gacgcagctg cccacggttc ccagttcatg caacaggtta gagttttgca aatacttaac     1140

aagaagggga tcgcttacgg tatcttgtgt gaggatgttg taaccggcgc caagttcacc     1200

attactggcc ccaaaaagtt tgttgttgct gccggtgctt tgaacactcc atctgtgttg     1260

gtcaactccg gcttcaagaa caagaacatc ggtaagaact taactttgca cccagtttct     1320

gtcgtgtttg gtgattttgg caaagacgtt caagcagacc acttccacaa ctccatcatg     1380

actgcccttt gttcagaagc cgctgattta gacggcaagg gccatggatg cagaattgaa     1440

accatcttga acgctccatt catccaggct tcattcttac catggagagg tagtaacgag     1500

gctagacgag acttgttgcg ttacaacaac atggtggcga tgttgctcct tagtcgtgac     1560

accaccagtg gttccgtttc tgctcatcca accaaacctg aagctttggt tgtcgagtac     1620

gacgtgaaca agtttgacag aaactcgatc ttgcaggcat tgttggtcac tgctgacttg     1680

ttgtatatcc aaggtgccaa gagaatcctt agtccacagg catgggtgcc aatttttgaa     1740

tccgacaagc caaaggataa gagatcaatc aaggacgagg actatgtcga atggagagcc     1800

aaggttgcca agattccttt cgacacctac ggctcacctt atggttcggc acatcaaatg     1860

tcttcttgcc gtatgtcagg taagggtcct aaatacggtg ctgttgacac cgatggtaga     1920

ttgtttgaat gttcgaatgt ttatgttgcc gatgcaagtc ttttgccaac tgcaagcggt     1980

gctaatccta tg                                                         1992


<210>  20
<211>  603
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis FAO1

<400>  20
cgaaaccacc gtcgaccaaa tcaaagacgt tattgctcct gacttccctg ctgacaagta       60

cgaagagtac gtcaggacat tcaccaaacc ctccgaaacc ccagggttca gggaaaccgt      120

ctacaacaca gtcaacgcaa acaccacgga cgcaatccac cagttcatta tcttgaccaa      180

tgttttggca tccagggtct tggctccagc tttgaccaac tcgttgacgc ctatcaagga      240

catgagcttg gaagaccgtg aaaaattgtt ggcctcgtgg cgcgactagg cggccgctag      300

atcttgcgaa gctccatctc gagttgttgt atatccaagg tgccaagaga atccttagtc      360

cacaggcatg ggtgccaatt tttgaatccg acaagccaaa ggataagaga tcaatcaagg      420

acgaggacta tgtcgaatgg agagccaagg ttgccaagat tcctttcgac acctacggct      480

caccttatgg ttcggcacat caaatgtctt cttgccgtat gtcaggtaag ggtcctaaat      540

acggtgctgt tgacaccgat ggtagattgt ttgaatgttc gaatgtttat gttgccgatg      600

caa                                                                    603


<210>  21
<211>  4755
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis FAO1

<400>  21
cgaaaccacc gtcgaccaaa tcaaagacgt tattgctcct gacttccctg ctgacaagta       60

cgaagagtac gtcaggacat tcaccaaacc ctccgaaacc ccagggttca gggaaaccgt      120

ctacaacaca gtcaacgcaa acaccacgga cgcaatccac cagttcatta tcttgaccaa      180

tgttttggca tccagggtct tggctccagc tttgaccaac tcgttgacgc ctatcaagga      240

catgagcttg gaagaccgtg aaaaattgtt ggcctcgtgg cgcgactagg cggccgctct      300

agaactagtg gatctgaagt tcctattctc tagaaagtat aggaacttcc tgcaggacca      360

cctttgattg taaatagtaa taattaccac ccttatctaa ttatttattt aacttattta      420

tttatttatt atacatatat acaaatctaa taaagtgaaa atctccccct tcacacttca      480

catatgttag gcgtcatcct gtgctcccga gaaccagtac cagtacatcg ctgtttcgtt      540

cgagacttga ggtctagttt tatacgtgaa gaggtcaatg ccgccgagag taaagccaca      600

ttttgcgtac aaattgcagg caggtacatt gttcgtttgt gtctctaatc gtatgccaag      660

gagctgtctg cttagtgccc actttttcgc aaattcgatg agactgtgcg cgactccttt      720

gcctcggtgc gtgtgcgaca caacaatgtg ttcgatagag gctagatcgt tccatgttga      780

gttgagttca atcttcccga caagctcttg gtcgatgaat gcgccatagc aagcagagtc      840

ttcatcagag tcatcatccg agatgtaatc cttccggtag gggctcacac ttctggtaga      900

tagttcaaag ccttggtcgg ataggtgcac atcgaacact tcacgaacaa tgaaatggtt      960

ctcagcatcc aatgtttccg ccacctgctc agggatcacc gaaattttca tatgagaacc     1020

gttatcgata actaaagcag caacttcttc tataaaaatg ggttagtatg acagtcattt     1080

aaataaggaa tttttcagtt ggcttggttt caattcaatg ttcgtttttt ttttttcttg     1140

ctgtgtttgt gtttgtgttg tttatagttg tgtgcactga gcgtcgaaaa aaaaaattca     1200

tagtgagccg ggaaatctgt atagcccaga taacaacaca agtccaaact agaaactcgt     1260

caaacaccaa aagcaatgtt gaatcaattg ccttgcacaa gtacacgtag gaaaacataa     1320

aacattgcaa ttttgaatat tgagcctttt gtcgtaacat tgattgatag gattactcac     1380

cgaatggttt tgaaaccact gccgacagat caatcaatca atcaaaaaac gtgaactttg     1440

aaaaagggga agaacagata cattgaagtt agccatttcc attgatcgtc acaacatatc     1500

tgataaatta ctttcaaaat tataagctga tgtgtgtgta ttattaatgt gacagtaaca     1560

tcccaaacga gaaatattat gtcgacaaca aaaaagtttg atctgaattg aaaatgaagt     1620

tttcccaccc tacccatttg tcatattgaa accaatcaac tgattaatca atcaattaga     1680

attgaagcta aactaaaaca taccaccgtc cattttgaat gattatattt ttttaatatt     1740

aatatcgaga taatgtttct aagaaagaaa gaaaaccagg agtgaaaatt agaaaaggaa     1800

aggaaaggaa aaaaagaaaa atctgaaaat atataaaaaa aaattgtttc gttggcaata     1860

aatcttggtg agaacagcga ccgaaagcaa ataagaacaa aatatgagtg tattacgttg     1920

aacaactaat taacgtgtgt gtatggatct ttttttcttt tttctcttta accgactata     1980

aacaacaaac atttttgggc agtgcacaca ctacttaata tacacagcat aaattacacg     2040

attagaaaca aattagctta ttaaaataac ctaatcaaac cgaatatttt atggtattat     2100

gagtaaacta tataatataa atagcacaca cccacaacaa caacaaagga aaactaaaag     2160

gttttttctt tttgaaaaga tcgttttctt tattattctc tagttttgac gctcgacatt     2220

ttatgatgga atgaatggga tgaatcatca aacaagagaa aatacccgtg acgaaaataa     2280

taaaataagt tcctctgata cagaagatga aaacaacaac aacaagatat agaaatgcct     2340

tgggtggcta ttttatagtc ttaacttttt aatgtatatt tgttttgttt ttttacataa     2400

taatacttta taaaagctaa gctaaattca agtaaaattt caatctctca aataaaacat     2460

ttttctcttt ttcttaaatt tagttttata tatttataaa atatacaaag atttttttaa     2520

aaaagtaaca agttatatat gtaataacaa aaagaagaat aacaagaata caaaaccaga     2580

tttccagatt tccagaattt cactcttata tgcgtctatt tatgtaggat gaaaggtagt     2640

ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac     2700

taccctttag ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg     2760

ctatcatttc ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt     2820

gatcaggtat tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct     2880

tatcgctcca atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg     2940

aggtcatcaa atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat     3000

aaggcgcatt tttcttcaaa gctttattgt acgatctgac taagttatct tttaataatt     3060

ggtattcctg tttattgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt     3120

cagaattcct caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa     3180

agaagtatat gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta     3240

ctcccaaata cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc     3300

tgaatcttcc acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt     3360

ttgtaaatct cgaagtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc     3420

tttcaccctc acttagaagt gctttaagca tttttttact gtggctattt cccttatctg     3480

cttcttccga tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt     3540

gatgtttttg tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta     3600

atgaggcttc caaaattgtt gctttttgcg tcttgtattt aaactggagt gatttattga     3660

caatatcgaa actcaacgaa ttgcttatga tagtattata gctcatgaat gtggctctct     3720

tgattgctgt tccgttatgt gtaatcatcc aacataaata ggttagttca gcagcacata     3780

atgctatttt ctcacctgaa ggtctttcaa acctttccac aaactgacga acaagcacct     3840

taggtggtgt tttacataat atatcaaatt gtggcatgtc gacgattatt agttaaacca     3900

ctgcaaaaag ttggggaaaa ttttgcccat ttttataccg tgtcttcgtc tatcgcctcc     3960

cccactcccc aatctttgaa ttattccgaa atattcagcg aacggggtgt acacaaaaac     4020

taacattctc aactgcataa tttgaaaaat ggcgtgggac aagaaaaaaa aaaaattctc     4080

aaccatagca atcatggaat acggtaaatt tgtgttgttc ggtgactcca tcacccagtt     4140

tagttgtacc cagtatggct ttcatccagc attacagaat gtgtatatcc gaaaattgga     4200

tgttattaac cgtggtttca gtggctacaa ctcagagcac gctagacaaa ttcttccaaa     4260

aattttagag tcggaaacca atatcaaatt gatgacaata ttttttggaa ctaacgatgc     4320

atacgactac atcaatgaaa tccagacagt cgagttagac agatataaag ataatttaag     4380

tgtaatggta cagatggtac tagacaaaaa tatcaaacca atcattattg gatccgaagt     4440

tcctattctc tagaaagtat aggaacttcc tcgagttgtt gtatatccaa ggtgccaaga     4500

gaatccttag tccacaggca tgggtgccaa tttttgaatc cgacaagcca aaggataaga     4560

gatcaatcaa ggacgaggac tatgtcgaat ggagagccaa ggttgccaag attcctttcg     4620

acacctacgg ctcaccttat ggttcggcac atcaaatgtc ttcttgccgt atgtcaggta     4680

agggtcctaa atacggtgct gttgacaccg atggtagatt gtttgaatgt tcgaatgttt     4740

atgttgccga tgcaa                                                      4755


<210>  22
<211>  4158
<212>  DNA
<213>  Candida tropicalis

<400>  22
tctcaccaag tacgagaacg agcttgttga tagagcttag acttgtcttt tgtatttgta       60

atctgacgtt gaccgtttga gtttttcctg tgatatcacg taaatctggc aaccagcttt      120

ctattttttt tgcaacactt tttctcttca ccactctcag aaccaatgcc accgaagaag      180

ggtctcaacc aggaggaaaa gctctcgtca atcctcgcct ggttccaaag cagccactgc      240

ttctatacgc tcaaagaggt tgaacagaag gctagcaaag cgtgcaagat ctcgtctatg      300

cagatcaagg acttggttgc aaccttagtc aacgaaggtt tagtggaaca ggaaaaatgt      360

gggaccacca acttgtactg gtcgttcccg tactcggaac acaaacgcaa gctacagaga      420

tacgagcagc taagacaatc cgttgccaaa cttcaagcga ataaaggcaa gttggcggaa      480

gagttgcgaa acgcgtgtgg tgagcgtgac atggacagca ataggctaaa ccggatgcaa      540

cagtgcgatc agcttgttca cgaggcggca cgcctccagg aggaactaaa actgtcgagg      600

cagagagata ccattgacga gttggttcag gccattgact tcttcaacga gctgatagag      660

accgtcctca gctacatcag ccatcagtcg gggaccagcg tgtcggtatt gaaaacggag      720

tttgagatac ccgcagaact agaagaggct ccccagataa acaatgccgg agttagtgcg      780

taaatcgagc atgcatacgt tggagagaaa tagagaaaca gatttccggt gaaacgctac      840

aacacagacg aggaatacag aatggaacat gacggaaata taatatccga ggaaagacga      900

aagtacgaca tggaactccg ttactgcaac atcgatcgtg ctagatacga catagaacaa      960

tgttgctatt acatggaaag ctgttgctac aatccagaat acggttgtac tcaagggaga     1020

tgaggctggg agccgagtgg tacataaata ggcatatagg accgtcactt ggtctaggat     1080

cgtgtagagg gtggaagagg taggcaagat ccattctaat ctactgagtg acggctaata     1140

tacgatcagc gttctcaggc gagcacagtc attcctcatt tctgtacata cgttgcccct     1200

ttatgttttt tttcacagga tgctcacgcc caacatttcc ccccacattt tattacccac     1260

attgagccgt caaatgcatt ttttttatcc gtcgcttgct aagacaaaat tccacatgct     1320

ttgtctcaga gtatataaac aacggggcaa aaaaacatgg ggttaatagc ttattcgtgg     1380

attgatattt ttatatttta gttcgcccct ttcgccacca agctcaattg gactatttgt     1440

cagtggtgta taagctagag attactagac tgcttttctg attcttgatt ttcccttttc     1500

attagttcca gtacctagag atgaatacct tcttgccaga cgtgctcgaa tacaaacacg     1560

tcgacaccct tttgttattg tgtgacggga tcatccacga aaccacagtc gatcagatca     1620

aggacgccat tgctcccgac ttccctgagg accagtacga ggagtatctc aagaccttca     1680

ccaagccatc tgagacccct gggttcagag aagccgtcta cgacacgatc aacgccaccc     1740

caaccgatgc cgtgcacatg tgtattgtct tgaccaccgc attggactcc agaatcttgg     1800

cccccacgtt gaccaactcg ttgacgccta tcaaggatat gaccttgaag gagcgtgaac     1860

aattgttggc ctcttggcgt gattccccga ttgcggcaaa gagaagattg ttcagattga     1920

tttcctcgct taccttgacg acgtttacga gattggccag cgaattgcac ttgaaagcca     1980

tccactaccc tggcagagac ttgcgtgaaa aggcgtatga aacccaggtg gttgaccctt     2040

tcaggtacct gtttatggag aaaccaaagt ttgacggcgc cgaattgtac ttgccagata     2100

tcgacgtcat catcattgga tcaggcgccg gtgctggtgt catggcccac actctcgcca     2160

acgacgggtt caagaccttg gttttggaaa agggaaagta tttcagcaac tccgagttga     2220

actttaatga cgctgatggc gtgaaagagt tgtaccaagg taaaggtgct ttggccacca     2280

ccaatcagca gatgtttatt cttgccggtt ccactttggg cggtggtacc actgtcaact     2340

ggtctgcttg ccttaaaaca ccatttaaag tgcgtaagga gtggtacgac gagtttggtc     2400

ttgaatttgc tgccgatgaa gcctacgaca aagcgcagga ttatgtttgg aaacaaatgg     2460

gtgcttcaac agatggaatc actcactcct tggccaacga agttgtggtt gaaggaggta     2520

agaagttggg ctacaagagc aaggaaattg agcagaacaa cggtggccac cctgaccacc     2580

catgtggttt ctgttacttg ggctgtaagt acggtattaa acagggttct gtgaataact     2640

ggtttagaga cgcagctgcc cacgggtcca agttcatgca acaagtcaga gttgtgcaaa     2700

tcctcaacaa gaatggcgtc gcttatggta tcttgtgtga ggatgtcgaa accggagtca     2760

ggttcactat tagtggcccc aaaaagtttg ttgtttctgc tggttctttg aacacgccaa     2820

ctgtgttgac caactccgga ttcaagaaca agcacattgg taagaacttg acgttgcacc     2880

cagtttccac cgtgtttggt gactttggca gagacgtgca agccgaccat ttccacaaat     2940

ctattatgac ttcgctttgt tacgaggttg ctgacttgga cggcaagggc cacggatgca     3000

gaatcgaaac catcttgaac gctccattca tccaagcttc tttgttgcca tggagaggaa     3060

gtgacgaggt cagaagagac ttgttgcgtt acaacaacat ggtggccatg ttgcttatca     3120

cgcgtgatac caccagtggt tcagtttctg ctgacccaaa gaagcccgac gctttgattg     3180

tcgactatga gattaacaag tttgacaaga atgccatctt gcaagctttc ttgatcactt     3240

ccgacatgtt gtacattgaa ggtgccaaga gaatcctcag tccacagcca tgggtgccaa     3300

tctttgagtc gaacaagcca aaggagcaaa gaacgatcaa ggacaaggac tatgttgagt     3360

ggagagccaa ggctgctaag atacctttcg acacctacgg ttctgcatat gggtccgcac     3420

atcaaatgtc cacctgtcgt atgtccggaa agggtcctaa atacggtgct gttgatactg     3480

atggtagatt gtttgaatgt tcgaatgtct atgttgctga tgctagtgtt ttgcctactg     3540

ccagcggtgc caacccaatg atatccacca tgacctttgc tagacagatt gcgttaggtt     3600

tggctgactc cttgaagacc aaacccaagt tgtagagaga cggaaatacg acacttatat     3660

actagatgta tcttacaatt tatattctcg atgatggctt ttactatctc ctatgttaca     3720

ctataatgac atcaccacaa cctctactac tgtctccagt atcctccttg ctgttgaccg     3780

tacccaccag cctgttgatt gaaccctgtg aactgtggtt gctgttgagc gtaccccacg     3840

ttagtgaact gcggttgttg ggcaaactgc tgtacgggct gttgctgctg ctgctgttgt     3900

tgttgttgtt gttgtcccgt gggctggttg tacaacgaca tgatgttctg cttgtttgtc     3960

tgttgggcaa ccaactgtgg gttattcatc tgcatcaact gctgctggtg ttgagggttg     4020

tttggatcca agtactcttg cccgttggcg tcgatataag aaatctgccc cgtgactggg     4080

tcagtgtact ggtatatctg tggcatgcca ccagcttgtg caggcatgcc ggttgccaat     4140

ggcacctgtg cttgcgtc                                                   4158


<210>  23
<211>  643
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis FAO2A

<400>  23
cgtctcatga ataccttctt gccagacgtg ctcgaataca aacacgtcga cacccttttg       60

ttattgtgtg acgggatcat ccacgaaacc acagtcgatc agatcaagga cgccattgct      120

cccgacttcc ctgaggacca gtacgaggag tatctcaaga ccttcaccaa gccatctgag      180

acccctgggt tcagagaagc cgtctacgac acgatcaacg ccaccccaac cgatgccgtg      240

cacatgtgta ttgtcttgac caccgcattg gactccagaa tcttggcccc cacgttgacc      300

aactaggcgg ccgctagatc ttgcgaagct ccatctcgag aaggacaagg actatgttga      360

gtggagagcc aaggctgcta agataccttt cgacacctac ggttctgcat atgggtccgc      420

acatcaaatg tccacctgtc gtatgtccgg aaagggtcct aaatacggtg ctgttgatac      480

tgatggtaga ttgtttgaat gttcgaatgt ctatgttgct gatgctagtg ttttgcctac      540

tgccagcggt gccaacccaa tgatatccac catgaccttt gctagacaga ttgcgttagg      600

tttggctgac tccttgaaga ccaaacccaa gttgtaggag acg                        643


<210>  24
<211>  4795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis FAO2A

<400>  24
cgtctcatga ataccttctt gccagacgtg ctcgaataca aacacgtcga cacccttttg       60

ttattgtgtg acgggatcat ccacgaaacc acagtcgatc agatcaagga cgccattgct      120

cccgacttcc ctgaggacca gtacgaggag tatctcaaga ccttcaccaa gccatctgag      180

acccctgggt tcagagaagc cgtctacgac acgatcaacg ccaccccaac cgatgccgtg      240

cacatgtgta ttgtcttgac caccgcattg gactccagaa tcttggcccc cacgttgacc      300

aactaggcgg ccgctctaga actagtggat ctgaagttcc tattctctag aaagtatagg      360

aacttcctgc aggaccacct ttgattgtaa atagtaataa ttaccaccct tatctaatta      420

tttatttaac ttatttattt atttattata catatataca aatctaataa agtgaaaatc      480

tcccccttca cacttcacat atgttaggcg tcatcctgtg ctcccgagaa ccagtaccag      540

tacatcgctg tttcgttcga gacttgaggt ctagttttat acgtgaagag gtcaatgccg      600

ccgagagtaa agccacattt tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgtc      660

tctaatcgta tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa ttcgatgaga      720

ctgtgcgcga ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc gatagaggct      780

agatcgttcc atgttgagtt gagttcaatc ttcccgacaa gctcttggtc gatgaatgcg      840

ccatagcaag cagagtcttc atcagagtca tcatccgaga tgtaatcctt ccggtagggg      900

ctcacacttc tggtagatag ttcaaagcct tggtcggata ggtgcacatc gaacacttca      960

cgaacaatga aatggttctc agcatccaat gtttccgcca cctgctcagg gatcaccgaa     1020

attttcatat gagaaccgtt atcgataact aaagcagcaa cttcttctat aaaaatgggt     1080

tagtatgaca gtcatttaaa taaggaattt ttcagttggc ttggtttcaa ttcaatgttc     1140

gttttttttt tttcttgctg tgtttgtgtt tgtgttgttt atagttgtgt gcactgagcg     1200

tcgaaaaaaa aaattcatag tgagccggga aatctgtata gcccagataa caacacaagt     1260

ccaaactaga aactcgtcaa acaccaaaag caatgttgaa tcaattgcct tgcacaagta     1320

cacgtaggaa aacataaaac attgcaattt tgaatattga gccttttgtc gtaacattga     1380

ttgataggat tactcaccga atggttttga aaccactgcc gacagatcaa tcaatcaatc     1440

aaaaaacgtg aactttgaaa aaggggaaga acagatacat tgaagttagc catttccatt     1500

gatcgtcaca acatatctga taaattactt tcaaaattat aagctgatgt gtgtgtatta     1560

ttaatgtgac agtaacatcc caaacgagaa atattatgtc gacaacaaaa aagtttgatc     1620

tgaattgaaa atgaagtttt cccaccctac ccatttgtca tattgaaacc aatcaactga     1680

ttaatcaatc aattagaatt gaagctaaac taaaacatac caccgtccat tttgaatgat     1740

tatatttttt taatattaat atcgagataa tgtttctaag aaagaaagaa aaccaggagt     1800

gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc tgaaaatata taaaaaaaaa     1860

ttgtttcgtt ggcaataaat cttggtgaga acagcgaccg aaagcaaata agaacaaaat     1920

atgagtgtat tacgttgaac aactaattaa cgtgtgtgta tggatctttt tttctttttt     1980

ctctttaacc gactataaac aacaaacatt tttgggcagt gcacacacta cttaatatac     2040

acagcataaa ttacacgatt agaaacaaat tagcttatta aaataaccta atcaaaccga     2100

atattttatg gtattatgag taaactatat aatataaata gcacacaccc acaacaacaa     2160

caaaggaaaa ctaaaaggtt ttttcttttt gaaaagatcg ttttctttat tattctctag     2220

ttttgacgct cgacatttta tgatggaatg aatgggatga atcatcaaac aagagaaaat     2280

acccgtgacg aaaataataa aataagttcc tctgatacag aagatgaaaa caacaacaac     2340

aagatataga aatgccttgg gtggctattt tatagtctta actttttaat gtatatttgt     2400

tttgtttttt tacataataa tactttataa aagctaagct aaattcaagt aaaatttcaa     2460

tctctcaaat aaaacatttt tctctttttc ttaaatttag ttttatatat ttataaaata     2520

tacaaagatt tttttaaaaa agtaacaagt tatatatgta ataacaaaaa gaagaataac     2580

aagaatacaa aaccagattt ccagatttcc agaatttcac tcttatatgc gtctatttat     2640

gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg     2700

tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt     2760

agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga     2820

aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc     2880

cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc     2940

cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt     3000

tatagcaaag attgaataag gcgcattttt cttcaaagct ttattgtacg atctgactaa     3060

gttatctttt aataattggt attcctgttt attgcttgaa gaattgccgg tcctatttac     3120

tcgttttagg actggttcag aattcctcaa aaattcatcc aaatatacaa gtggatcgat     3180

cctacccctt gcgctaaaga agtatatgtg cctactaacg cttgtctttg tctctgtcac     3240

taaacactgg attattactc ccaaatactt attttggact aatttaaatg atttcggatc     3300

aacgttctta atatcgctga atcttccaca attgatgaaa gtagctagga agaggaattg     3360

gtataaagtt tttgtttttg taaatctcga agtatactca aacgaattta gtattttctc     3420

agtgatctcc cagatgcttt caccctcact tagaagtgct ttaagcattt ttttactgtg     3480

gctatttccc ttatctgctt cttccgatga ttcgaactgt aattgcaaac tacttacaat     3540

atcagtgata tcagattgat gtttttgtcc atagtaagga ataattgtaa attcccaagc     3600

aggaatcaat ttctttaatg aggcttccaa aattgttgct ttttgcgtct tgtatttaaa     3660

ctggagtgat ttattgacaa tatcgaaact caacgaattg cttatgatag tattatagct     3720

catgaatgtg gctctcttga ttgctgttcc gttatgtgta atcatccaac ataaataggt     3780

tagttcagca gcacataatg ctattttctc acctgaaggt ctttcaaacc tttccacaaa     3840

ctgacgaaca agcaccttag gtggtgtttt acataatata tcaaattgtg gcatgtcgac     3900

gattattagt taaaccactg caaaaagttg gggaaaattt tgcccatttt tataccgtgt     3960

cttcgtctat cgcctccccc actccccaat ctttgaatta ttccgaaata ttcagcgaac     4020

ggggtgtaca caaaaactaa cattctcaac tgcataattt gaaaaatggc gtgggacaag     4080

aaaaaaaaaa aattctcaac catagcaatc atggaatacg gtaaatttgt gttgttcggt     4140

gactccatca cccagtttag ttgtacccag tatggctttc atccagcatt acagaatgtg     4200

tatatccgaa aattggatgt tattaaccgt ggtttcagtg gctacaactc agagcacgct     4260

agacaaattc ttccaaaaat tttagagtcg gaaaccaata tcaaattgat gacaatattt     4320

tttggaacta acgatgcata cgactacatc aatgaaatcc agacagtcga gttagacaga     4380

tataaagata atttaagtgt aatggtacag atggtactag acaaaaatat caaaccaatc     4440

attattggat ccgaagttcc tattctctag aaagtatagg aacttcctcg agaaggacaa     4500

ggactatgtt gagtggagag ccaaggctgc taagatacct ttcgacacct acggttctgc     4560

atatgggtcc gcacatcaaa tgtccacctg tcgtatgtcc ggaaagggtc ctaaatacgg     4620

tgctgttgat actgatggta gattgtttga atgttcgaat gtctatgttg ctgatgctag     4680

tgttttgcct actgccagcg gtgccaaccc aatgatatcc accatgacct ttgctagaca     4740

gattgcgtta ggtttggctg actccttgaa gaccaaaccc aagttgtagg agacg          4795


<210>  25
<211>  3753
<212>  DNA
<213>  Candida tropicalis

<400>  25
tgttgataga gcttagactt gtgttttgta tttgtaatct gacgttgatc gtttgatatt       60

ttcctgtgat atcacgtaaa ttcggcaacc aactttttac tttttgcaac actttctctt      120

caccactctc agaaccaatg ccaccgaaga agggtctcag ccaggaggaa aagctctcgg      180

cactcctcac ctggttccaa gccagtcatt gcttctacac actcaaggag gttgaacaga      240

aggcgagcaa agcgtgcaag atctcgtcta tgcagatcaa ggacttggtt gcaagcttag      300

tcaacgaagg tttggtagaa caggaaaagt gtgggaccac aaacttgtac tggtcgttcc      360

agtactcgga attcaaacgg aagctacaga gatacgggca gctaagacaa tcagccgcca      420

aacttcaagc ggataaaggc aagttggcgg aagagttgcg aaacgcatgt ggtgaacggg      480

acatggacaa caataggcaa gaccggatgc aacaatacga tcaccttgtt aacgaggcgg      540

cacgtctcca ggaggaacta aaactgtcaa ggcagataga taccattgac gagttagttc      600

aggccattga tttcttcaac gagctgatag agaccgtcct cagctacatc agccatcagt      660

cagggaccag cgtgtcgata ttgaaaacgg agtttgagat acccgcagaa ctagaagagg      720

ccccccagat aagcaatgcc ggagttagtg cgtaaatcga gcaggcatac attgcccctt      780

tgtatttttt cacaggatgc tcaccccacc acgcccaaca tttcccccca cattttatta      840

cccacattga gccgtcaaat gcattttttt atccgtcgct agctaaacca aaattccaca      900

tgcgttgcct cagagtatat aaacaacggg gcaaaaaaca tgggattaat agcttatttg      960

tggattgata tttttatatt ttagttcgcc ccttctacga ccaagctcaa ttggactatt     1020

tgtcagtggt gtataagcta gagattacta gactgctttt ctgattcttg atcatcccct     1080

tagttccagt gcctagagat gaataccttc ttgccagacg tgctcgaata caaacacgtc     1140

gatacccttt tgttattatg tgacgggatc atccacgaaa ccacagtcga ccagatcagg     1200

gacgccattg ctcccgactt ccctgaagac cagtacgagg agtatctcaa gaccttcacc     1260

aagccatctg agacccctgg gttcagagaa gccgtctacg acacgatcaa cagcacccca     1320

accgaggctg tgcacatgtg tattgtattg accaccgcat tggactcgag aatcttggcc     1380

cccacgttga ccaactcgtt gacgcctatc aaggatatga ccttgaaaga gcgtgaacaa     1440

ttgttggctg cctggcgtga ttccccgatc gcggccaaga gaagattgtt cagattgatt     1500

tcctcactta ccttgacgac ctttacgaga ttggccagcg acttgcactt gagagccatc     1560

cactaccctg gcagagactt gcgtgaaaag gcatatgaaa cccaggtggt tgaccctttc     1620

aggtacctgt ttatggaaaa accaaagttt gacggcaccg agttgtactt gccagatatc     1680

gacgtcatca tcattggatc cggtgccggt gctggtgtca tggcccacac tttagccaac     1740

gacgggtaca agaccttggt tttggaaaag ggaaagtatt tcagcaactc cgagttgaac     1800

tttaatgatg ccgatggtat gaaagagttg taccaaggta aatgtgcgtt gaccaccacg     1860

aaccagcaga tgtttattct tgccggttcc actttgggcg gtggtaccac tgttaactgg     1920

tctgcttgtc ttaaaacacc atttaaagtg cgtaaggagt ggtacgacga gtttggtctt     1980

gaatttgctg ccgacgaagc ctacgacaaa gcacaagact atgtttggaa acaaatgggc     2040

gcttctaccg aaggaatcac tcactctttg gcgaacgcgg ttgtggttga aggaggtaag     2100

aagttgggtt acaagagcaa ggaaatcgag cagaacaatg gtggccatcc tgaccacccc     2160

tgtggtttct gttacttggg ctgtaagtac ggtattaagc agggttctgt gaataactgg     2220

tttagagacg cagctgccca cgggtccaag ttcatgcaac aagtcagagt tgtgcaaatc     2280

ctccacaata aaggcgtcgc ttatggcatc ttgtgtgagg atgtcgagac cggagtcaaa     2340

ttcactatca gtggccccaa aaagtttgtt gtttctgcag gttctttgaa cacgccaacg     2400

gtgttgacca actccggatt caagaacaaa cacatcggta agaacttgac gttgcaccca     2460

gtttcgaccg tgtttggtga ctttggcaga gacgtgcaag ccgaccattt ccacaaatct     2520

attatgactt cgctctgtta cgaagtcgct gacttggacg gcaagggcca cggatgcaga     2580

atcgagacca tcttgaacgc tccattcatc caagcttctt tgttgccatg gagaggaagc     2640

gacgaggtca gaagagactt gttgcgttac aacaacatgg tggccatgtt gcttatcacc     2700

cgtgacacca ccagtggttc agtttctgct gacccaaaga agcccgacgc tttgattgtc     2760

gactatgaca tcaacaagtt tgacaagaat gccatcttgc aagctttctt gatcacctcc     2820

gacatgttgt acatcgaagg tgccaagaga atcctcagtc cacaggcatg ggtgccaatc     2880

tttgagtcga acaagccaaa ggagcaaaga acaatcaagg acaaggacta tgtcgaatgg     2940

agagccaagg ctgccaagat acctttcgac acctacggtt ctgcctatgg gtccgcacat     3000

caaatgtcca cctgtcgtat gtccggaaag ggtcctaaat acggcgccgt tgataccgat     3060

ggtagattgt ttgaatgttc gaatgtctat gttgctgatg ctagtgtttt gcctactgcc     3120

agcggtgcca acccaatgat ctccaccatg acgtttgcta gacagattgc gttaggtttg     3180

gctgactctt tgaagaccaa acccaagttg tagagagaga cagaaatacg acacttatat     3240

actagatgta tcttacaatt tatattttcg atgatggctt ttactatctc ctatgttaca     3300

ctataatgac atcaccacat cttctactac tgtctccagt atcctccttg ctgttgaccg     3360

tatccaccag cctgttggtt gaaccccgtg aactgtggtt gctgttgagc gtaccccacg     3420

ttagtgaact gcggttgttg ggtaaactgc tgtacgggct gttgttgctg ttgctgttgt     3480

tgctgttgtt gctgttgttg ctgttgttgc tgttgttgtt gttgtcccgt tggctggttg     3540

tacaacgaca tgatgttctg cttgtttgtc tgctgggcaa ccaactgtgg gttattcatc     3600

tgcatcaact gctgctggtg ctgagggttg tttggatcca agtactcctg cccgttggcg     3660

tcgatataag aaatctgccc cgtgactggg tcagtgtact ggtatatctg tggcatgcca     3720

cccgcttgtg caggcatgcc ggttgccaat ggc                                  3753


<210>  26
<211>  616
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis FAO2B

<400>  26
cgtctcatga ataccttctt gccagacgtg ctcgaataca aacacgtcga tacccttttg       60

ttattatgtg acgggatcat ccacgaaacc acagtcgacc agatcaggga cgccattgct      120

cccgacttcc ctgaagacca gtacgaggag tatctcaaga ccttcaccaa gccatctgag      180

acccctgggt tcagagaagc cgtctacgac acgatcaaca gcaccccaac cgaggctgtg      240

cacatgtgta ttgtattgac caccgcattg gactcgtagg cggccgctag atcttgcgaa      300

gctccatctc gagaaggaca aggactatgt cgaatggaga gccaaggctg ccaagatacc      360

tttcgacacc tacggttctg cctatgggtc cgcacatcaa atgtccacct gtcgtatgtc      420

cggaaagggt cctaaatacg gcgccgttga taccgatggt agattgtttg aatgttcgaa      480

tgtctatgtt gctgatgcta gtgttttgcc tactgccagc ggtgccaacc caatgatctc      540

caccatgacg tttgctagac agattgcgtt aggtttggct gactctttga agaccaaacc      600

caagttgtag gagacg                                                      616


<210>  27
<211>  4768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis FAO2B

<400>  27
cgtctcatga ataccttctt gccagacgtg ctcgaataca aacacgtcga tacccttttg       60

ttattatgtg acgggatcat ccacgaaacc acagtcgacc agatcaggga cgccattgct      120

cccgacttcc ctgaagacca gtacgaggag tatctcaaga ccttcaccaa gccatctgag      180

acccctgggt tcagagaagc cgtctacgac acgatcaaca gcaccccaac cgaggctgtg      240

cacatgtgta ttgtattgac caccgcattg gactcgtagg cggccgctct agaactagtg      300

gatctgaagt tcctattctc tagaaagtat aggaacttcc tgcaggacca cctttgattg      360

taaatagtaa taattaccac ccttatctaa ttatttattt aacttattta tttatttatt      420

atacatatat acaaatctaa taaagtgaaa atctccccct tcacacttca catatgttag      480

gcgtcatcct gtgctcccga gaaccagtac cagtacatcg ctgtttcgtt cgagacttga      540

ggtctagttt tatacgtgaa gaggtcaatg ccgccgagag taaagccaca ttttgcgtac      600

aaattgcagg caggtacatt gttcgtttgt gtctctaatc gtatgccaag gagctgtctg      660

cttagtgccc actttttcgc aaattcgatg agactgtgcg cgactccttt gcctcggtgc      720

gtgtgcgaca caacaatgtg ttcgatagag gctagatcgt tccatgttga gttgagttca      780

atcttcccga caagctcttg gtcgatgaat gcgccatagc aagcagagtc ttcatcagag      840

tcatcatccg agatgtaatc cttccggtag gggctcacac ttctggtaga tagttcaaag      900

ccttggtcgg ataggtgcac atcgaacact tcacgaacaa tgaaatggtt ctcagcatcc      960

aatgtttccg ccacctgctc agggatcacc gaaattttca tatgagaacc gttatcgata     1020

actaaagcag caacttcttc tataaaaatg ggttagtatg acagtcattt aaataaggaa     1080

tttttcagtt ggcttggttt caattcaatg ttcgtttttt ttttttcttg ctgtgtttgt     1140

gtttgtgttg tttatagttg tgtgcactga gcgtcgaaaa aaaaaattca tagtgagccg     1200

ggaaatctgt atagcccaga taacaacaca agtccaaact agaaactcgt caaacaccaa     1260

aagcaatgtt gaatcaattg ccttgcacaa gtacacgtag gaaaacataa aacattgcaa     1320

ttttgaatat tgagcctttt gtcgtaacat tgattgatag gattactcac cgaatggttt     1380

tgaaaccact gccgacagat caatcaatca atcaaaaaac gtgaactttg aaaaagggga     1440

agaacagata cattgaagtt agccatttcc attgatcgtc acaacatatc tgataaatta     1500

ctttcaaaat tataagctga tgtgtgtgta ttattaatgt gacagtaaca tcccaaacga     1560

gaaatattat gtcgacaaca aaaaagtttg atctgaattg aaaatgaagt tttcccaccc     1620

tacccatttg tcatattgaa accaatcaac tgattaatca atcaattaga attgaagcta     1680

aactaaaaca taccaccgtc cattttgaat gattatattt ttttaatatt aatatcgaga     1740

taatgtttct aagaaagaaa gaaaaccagg agtgaaaatt agaaaaggaa aggaaaggaa     1800

aaaaagaaaa atctgaaaat atataaaaaa aaattgtttc gttggcaata aatcttggtg     1860

agaacagcga ccgaaagcaa ataagaacaa aatatgagtg tattacgttg aacaactaat     1920

taacgtgtgt gtatggatct ttttttcttt tttctcttta accgactata aacaacaaac     1980

atttttgggc agtgcacaca ctacttaata tacacagcat aaattacacg attagaaaca     2040

aattagctta ttaaaataac ctaatcaaac cgaatatttt atggtattat gagtaaacta     2100

tataatataa atagcacaca cccacaacaa caacaaagga aaactaaaag gttttttctt     2160

tttgaaaaga tcgttttctt tattattctc tagttttgac gctcgacatt ttatgatgga     2220

atgaatggga tgaatcatca aacaagagaa aatacccgtg acgaaaataa taaaataagt     2280

tcctctgata cagaagatga aaacaacaac aacaagatat agaaatgcct tgggtggcta     2340

ttttatagtc ttaacttttt aatgtatatt tgttttgttt ttttacataa taatacttta     2400

taaaagctaa gctaaattca agtaaaattt caatctctca aataaaacat ttttctcttt     2460

ttcttaaatt tagttttata tatttataaa atatacaaag atttttttaa aaaagtaaca     2520

agttatatat gtaataacaa aaagaagaat aacaagaata caaaaccaga tttccagatt     2580

tccagaattt cactcttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc     2640

ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag     2700

ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc     2760

ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat     2820

tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca     2880

atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa     2940

atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt     3000

tttcttcaaa gctttattgt acgatctgac taagttatct tttaataatt ggtattcctg     3060

tttattgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcct     3120

caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa agaagtatat     3180

gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccaaata     3240

cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc     3300

acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct     3360

cgaagtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc tttcaccctc     3420

acttagaagt gctttaagca tttttttact gtggctattt cccttatctg cttcttccga     3480

tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg     3540

tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta atgaggcttc     3600

caaaattgtt gctttttgcg tcttgtattt aaactggagt gatttattga caatatcgaa     3660

actcaacgaa ttgcttatga tagtattata gctcatgaat gtggctctct tgattgctgt     3720

tccgttatgt gtaatcatcc aacataaata ggttagttca gcagcacata atgctatttt     3780

ctcacctgaa ggtctttcaa acctttccac aaactgacga acaagcacct taggtggtgt     3840

tttacataat atatcaaatt gtggcatgtc gacgattatt agttaaacca ctgcaaaaag     3900

ttggggaaaa ttttgcccat ttttataccg tgtcttcgtc tatcgcctcc cccactcccc     3960

aatctttgaa ttattccgaa atattcagcg aacggggtgt acacaaaaac taacattctc     4020

aactgcataa tttgaaaaat ggcgtgggac aagaaaaaaa aaaaattctc aaccatagca     4080

atcatggaat acggtaaatt tgtgttgttc ggtgactcca tcacccagtt tagttgtacc     4140

cagtatggct ttcatccagc attacagaat gtgtatatcc gaaaattgga tgttattaac     4200

cgtggtttca gtggctacaa ctcagagcac gctagacaaa ttcttccaaa aattttagag     4260

tcggaaacca atatcaaatt gatgacaata ttttttggaa ctaacgatgc atacgactac     4320

atcaatgaaa tccagacagt cgagttagac agatataaag ataatttaag tgtaatggta     4380

cagatggtac tagacaaaaa tatcaaacca atcattattg gatccgaagt tcctattctc     4440

tagaaagtat aggaacttcc tcgagaagga caaggactat gtcgaatgga gagccaaggc     4500

tgccaagata cctttcgaca cctacggttc tgcctatggg tccgcacatc aaatgtccac     4560

ctgtcgtatg tccggaaagg gtcctaaata cggcgccgtt gataccgatg gtagattgtt     4620

tgaatgttcg aatgtctatg ttgctgatgc tagtgttttg cctactgcca gcggtgccaa     4680

cccaatgatc tccaccatga cgtttgctag acagattgcg ttaggtttgg ctgactcttt     4740

gaagaccaaa cccaagttgt aggagacg                                        4768


<210>  28
<211>  4115
<212>  DNA
<213>  Candida tropicalis

<400>  28
catatgcgct aatcttcttt ttctttttat cacaggagaa actatcccac ccccacttcg       60

aaacacaatg acaactcctg cgtaacttgc aaattcttgt ctgactaatt gaaaactccg      120

gacgagtcag acctccagtc aaacggacag acagacaaac acttggtgcg atgttcatac      180

ctacagacat gtcaacgggt gttagacgac ggtttcttgc aaagacaggt gttggcatct      240

cgtacgatgg caactgcagg aggtgtcgac ttctccttta ggcaatagaa aaagactaag      300

agaacagcgt ttttacaggt tgcattggtt aatgtagtat ttttttagtc ccagcattct      360

gtgggttgct ctgggtttct agaataggaa atcacaggag aatgcaaatt cagatggaag      420

aacaaagaga taaaaaacaa aaaaaaactg agttttgcac caatagaatg tttgatgata      480

tcatccactc gctaaacgaa tcatgtgggt gatcttctct ttagttttgg tctatcataa      540

aacacatgaa agtgaaatcc aaatacacta cactccgggt attgtccttc gttttacaga      600

tgtctcattg tcttactttt gaggtcatag gagttgcctg tgagagatca cagagattat      660

cacactcaca tttatcgtag tttcctatct catgctgtgt gtctctggtt ggttcatgag      720

tttggattgt tgtacattaa aggaatcgct ggaaagcaaa gctaactaaa ttttctttgt      780

cacaggtaca ctaacctgta aaacttcact gccacgccag tctttcctga ttgggcaagt      840

gcacaaacta caacctgcaa aacagcactc cgcttgtcac aggttgtctc ctctcaacca      900

acaaaaaaat aagattaaac tttctttgct catgcatcaa tcggagttat ctctgaaaga      960

gttgcctttg tgtaatgtgt gccaaactca aactgcaaaa ctaaccacag aatgatttcc     1020

ctcacaatta tataaactca cccacatttc cacagaccgt aatttcatgt ctcactttct     1080

cttttgctct tcttttactt agtcaggttt gataacttcc ttttttatta ccctatctta     1140

tttatttatt tattcattta taccaaccaa ccaaccatgg ccacacaaga aatcatcgat     1200

tctgtacttc cgtacttgac caaatggtac actgtgatta ctgcagcagt attagtcttc     1260

cttatctcca caaacatcaa gaactacgtc aaggcaaaga aattgaaatg tgtcgatcca     1320

ccatacttga aggatgccgg tctcactggt attctgtctt tgatcgccgc catcaaggcc     1380

aagaacgacg gtagattggc taactttgcc gatgaagttt tcgacgagta cccaaaccac     1440

accttctact tgtctgttgc cggtgctttg aagattgtca tgactgttga cccagaaaac     1500

atcaaggctg tcttggccac ccaattcact gacttctcct tgggtaccag acacgcccac     1560

tttgctcctt tgttgggtga cggtatcttc accttggacg gagaaggttg gaagcactcc     1620

agagctatgt tgagaccaca gtttgctaga gaccagattg gacacgttaa agccttggaa     1680

ccacacatcc aaatcatggc taagcagatc aagttgaacc agggaaagac tttcgatatc     1740

caagaattgt tctttagatt taccgtcgac accgctactg agttcttgtt tggtgaatcc     1800

gttcactcct tgtacgatga aaaattgggc atcccaactc caaacgaaat cccaggaaga     1860

gaaaactttg ccgctgcttt caacgtttcc caacactact tggccaccag aagttactcc     1920

cagacttttt actttttgac caaccctaag gaattcagag actgtaacgc caaggtccac     1980

cacttggcca agtactttgt caacaaggcc ttgaacttta ctcctgaaga actcgaagag     2040

aaatccaagt ccggttacgt tttcttgtac gaattggtta agcaaaccag agatccaaag     2100

gtcttgcaag atcaattgtt gaacattatg gttgccggaa gagacaccac tgccggtttg     2160

ttgtcctttg ctttgtttga attggctaga cacccagaga tgtggtccaa gttgagagaa     2220

gaaatcgaag ttaactttgg tgttggtgaa gactcccgcg ttgaagaaat taccttcgaa     2280

gccttgaaga gatgtgaata cttgaaggct atccttaacg aaaccttgcg tatgtaccca     2340

tctgttcctg tcaactttag aaccgccacc agagacacca ctttgccaag aggtggtggt     2400

gctaacggta ccgacccaat ctacattcct aaaggctcca ctgttgctta cgttgtctac     2460

aagacccacc gtttggaaga atactacggt aaggacgcta acgacttcag accagaaaga     2520

tggtttgaac catctactaa gaagttgggc tgggcttatg ttccattcaa cggtggtcca     2580

agagtctgct tgggtcaaca attcgccttg actgaagctt cttatgtgat cactagattg     2640

gcccagatgt ttgaaactgt ctcatctgat ccaggtctcg aataccctcc accaaagtgt     2700

attcacttga ccatgagtca caacgatggt gtctttgtca agatgtaaag tagtcgatgc     2760

tgggtattcg attacatgtg tataggaaga ttttggtttt ttattcgttc ttttttttaa     2820

tttttgttaa attagtttag agatttcatt aatacataga tgggtgctat ttccgaaact     2880

ttacttctat cccctgtatc ccttattatc cctctcagtc acatgattgc tgtaattgtc     2940

gtgcaggaca caaactccct aacggactta aaccataaac aagctcagaa ccataagccg     3000

acatcactcc ttcttctctc ttctccaacc aatagcatgg acagacccac cctcctatcc     3060

gaatcgaaga cccttattga ctccataccc acctggaagc ccctcaagcc acacacgtca     3120

tccagcccac ccatcaccac atccctctac tcgacaacgt ccaaagacgg cgagttctgg     3180

tgtgcccgga aatcagccat cccggccaca tacaagcagc cgttgattgc gtgcatactc     3240

ggcgagccca caatgggagc cacgcattcg gaccatgaag caaagtacat tcacgagatc     3300

acgggtgttt cagtgtcgca gattgagaag ttcgacgatg gatggaagta cgatctcgtt     3360

gcggattacg acttcggtgg gttgttatct aaacgaagat tctatgagac gcagcatgtg     3420

tttcggttcg aggattgtgc gtacgtcatg agtgtgcctt ttgatggacc caaggaggaa     3480

ggttacgtgg ttgggacgta cagatccatt gaaaggttga gctggggtaa agacggggac     3540

gtggagtgga ccatggcgac gacgtcggat cctggtgggt ttatcccgca atggataact     3600

cgattgagca tccctggagc aatcgcaaaa gatgtgccta gtgtattaaa ctacatacag     3660

aaataaaaac gtgtcttgat tcattggttt ggttcttgtt gggttccgag ccaatatttc     3720

acatcatctc ctaaattctc caagaatccc aacgtagcgt agtccagcac gccctctgag     3780

atcttattta atatcgactt ctcaaccacc ggtggaatcc cgttcagacc attgttacct     3840

gtagtgtgtt tgctcttgtt cttgatgaca atgatgtatt tgtcacgata cctgaaataa     3900

taaaacatcc agtcattgag cttattactc gtgaacttat gaaagaactc attcaagccg     3960

ttcccaaaaa acccagaatt gaagatcttg ctcaactggt catgcaagta gtagatcgcc     4020

atgatctgat actttaccaa gctatcctct ccaagttctc ccacgtacgg caagtacggc     4080

aacgagctct ggaagctttg ttgtttgggg tcata                                4115


<210>  29
<211>  649
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis CYP52A12

<400>  29
cgtctctcac aggttgtctc ctctcaacca acaaaaaaat aagattaaac tttctttgct       60

catgcatcaa tcggagttat ctctgaaaga gttgcctttg tgtaatgtgt gccaaactca      120

aactgcaaaa ctaaccacag aatgatttcc ctcacaatta tataaactca cccacatttc      180

cacagaccgt aatttcatgt ctcactttct cttttgctct tcttttactt agtcaggttt      240

gataacttcc ttttttatta ccctatctta tttatttatt tattcattta taccaaccaa      300

ccaacctagg cggccgctag atcttgcgaa gctccatctc gagagtagtc gatgctgggt      360

attcgattac atgtgtatag gaagattttg gttttttatt cgttcttttt tttaattttt      420

gttaaattag tttagagatt tcattaatac atagatgggt gctatttccg aaactttact      480

tctatcccct gtatccctta ttatccctct cagtcacatg attgctgtaa ttgtcgtgca      540

ggacacaaac tccctaacgg acttaaacca taaacaagct cagaaccata agccgacatc      600

actccttctt ctctcttctc caaccaatag catggacaga cccgagacg                  649


<210>  30
<211>  4801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A12

<400>  30
cgtctctcac aggttgtctc ctctcaacca acaaaaaaat aagattaaac tttctttgct       60

catgcatcaa tcggagttat ctctgaaaga gttgcctttg tgtaatgtgt gccaaactca      120

aactgcaaaa ctaaccacag aatgatttcc ctcacaatta tataaactca cccacatttc      180

cacagaccgt aatttcatgt ctcactttct cttttgctct tcttttactt agtcaggttt      240

gataacttcc ttttttatta ccctatctta tttatttatt tattcattta taccaaccaa      300

ccaacctagg cggccgctct agaactagtg gatctgaagt tcctattctc tagaaagtat      360

aggaacttcc tgcaggacca cctttgattg taaatagtaa taattaccac ccttatctaa      420

ttatttattt aacttattta tttatttatt atacatatat acaaatctaa taaagtgaaa      480

atctccccct tcacacttca catatgttag gcgtcatcct gtgctcccga gaaccagtac      540

cagtacatcg ctgtttcgtt cgagacttga ggtctagttt tatacgtgaa gaggtcaatg      600

ccgccgagag taaagccaca ttttgcgtac aaattgcagg caggtacatt gttcgtttgt      660

gtctctaatc gtatgccaag gagctgtctg cttagtgccc actttttcgc aaattcgatg      720

agactgtgcg cgactccttt gcctcggtgc gtgtgcgaca caacaatgtg ttcgatagag      780

gctagatcgt tccatgttga gttgagttca atcttcccga caagctcttg gtcgatgaat      840

gcgccatagc aagcagagtc ttcatcagag tcatcatccg agatgtaatc cttccggtag      900

gggctcacac ttctggtaga tagttcaaag ccttggtcgg ataggtgcac atcgaacact      960

tcacgaacaa tgaaatggtt ctcagcatcc aatgtttccg ccacctgctc agggatcacc     1020

gaaattttca tatgagaacc gttatcgata actaaagcag caacttcttc tataaaaatg     1080

ggttagtatg acagtcattt aaataaggaa tttttcagtt ggcttggttt caattcaatg     1140

ttcgtttttt ttttttcttg ctgtgtttgt gtttgtgttg tttatagttg tgtgcactga     1200

gcgtcgaaaa aaaaaattca tagtgagccg ggaaatctgt atagcccaga taacaacaca     1260

agtccaaact agaaactcgt caaacaccaa aagcaatgtt gaatcaattg ccttgcacaa     1320

gtacacgtag gaaaacataa aacattgcaa ttttgaatat tgagcctttt gtcgtaacat     1380

tgattgatag gattactcac cgaatggttt tgaaaccact gccgacagat caatcaatca     1440

atcaaaaaac gtgaactttg aaaaagggga agaacagata cattgaagtt agccatttcc     1500

attgatcgtc acaacatatc tgataaatta ctttcaaaat tataagctga tgtgtgtgta     1560

ttattaatgt gacagtaaca tcccaaacga gaaatattat gtcgacaaca aaaaagtttg     1620

atctgaattg aaaatgaagt tttcccaccc tacccatttg tcatattgaa accaatcaac     1680

tgattaatca atcaattaga attgaagcta aactaaaaca taccaccgtc cattttgaat     1740

gattatattt ttttaatatt aatatcgaga taatgtttct aagaaagaaa gaaaaccagg     1800

agtgaaaatt agaaaaggaa aggaaaggaa aaaaagaaaa atctgaaaat atataaaaaa     1860

aaattgtttc gttggcaata aatcttggtg agaacagcga ccgaaagcaa ataagaacaa     1920

aatatgagtg tattacgttg aacaactaat taacgtgtgt gtatggatct ttttttcttt     1980

tttctcttta accgactata aacaacaaac atttttgggc agtgcacaca ctacttaata     2040

tacacagcat aaattacacg attagaaaca aattagctta ttaaaataac ctaatcaaac     2100

cgaatatttt atggtattat gagtaaacta tataatataa atagcacaca cccacaacaa     2160

caacaaagga aaactaaaag gttttttctt tttgaaaaga tcgttttctt tattattctc     2220

tagttttgac gctcgacatt ttatgatgga atgaatggga tgaatcatca aacaagagaa     2280

aatacccgtg acgaaaataa taaaataagt tcctctgata cagaagatga aaacaacaac     2340

aacaagatat agaaatgcct tgggtggcta ttttatagtc ttaacttttt aatgtatatt     2400

tgttttgttt ttttacataa taatacttta taaaagctaa gctaaattca agtaaaattt     2460

caatctctca aataaaacat ttttctcttt ttcttaaatt tagttttata tatttataaa     2520

atatacaaag atttttttaa aaaagtaaca agttatatat gtaataacaa aaagaagaat     2580

aacaagaata caaaaccaga tttccagatt tccagaattt cactcttata tgcgtctatt     2640

tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta     2700

tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg     2760

attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg     2820

agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat acgttgtcct     2880

ggccacggca gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag     2940

gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt     3000

ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctttattgt acgatctgac     3060

taagttatct tttaataatt ggtattcctg tttattgctt gaagaattgc cggtcctatt     3120

tactcgtttt aggactggtt cagaattcct caaaaattca tccaaatata caagtggatc     3180

gatcctaccc cttgcgctaa agaagtatat gtgcctacta acgcttgtct ttgtctctgt     3240

cactaaacac tggattatta ctcccaaata cttattttgg actaatttaa atgatttcgg     3300

atcaacgttc ttaatatcgc tgaatcttcc acaattgatg aaagtagcta ggaagaggaa     3360

ttggtataaa gtttttgttt ttgtaaatct cgaagtatac tcaaacgaat ttagtatttt     3420

ctcagtgatc tcccagatgc tttcaccctc acttagaagt gctttaagca tttttttact     3480

gtggctattt cccttatctg cttcttccga tgattcgaac tgtaattgca aactacttac     3540

aatatcagtg atatcagatt gatgtttttg tccatagtaa ggaataattg taaattccca     3600

agcaggaatc aatttcttta atgaggcttc caaaattgtt gctttttgcg tcttgtattt     3660

aaactggagt gatttattga caatatcgaa actcaacgaa ttgcttatga tagtattata     3720

gctcatgaat gtggctctct tgattgctgt tccgttatgt gtaatcatcc aacataaata     3780

ggttagttca gcagcacata atgctatttt ctcacctgaa ggtctttcaa acctttccac     3840

aaactgacga acaagcacct taggtggtgt tttacataat atatcaaatt gtggcatgtc     3900

gacgattatt agttaaacca ctgcaaaaag ttggggaaaa ttttgcccat ttttataccg     3960

tgtcttcgtc tatcgcctcc cccactcccc aatctttgaa ttattccgaa atattcagcg     4020

aacggggtgt acacaaaaac taacattctc aactgcataa tttgaaaaat ggcgtgggac     4080

aagaaaaaaa aaaaattctc aaccatagca atcatggaat acggtaaatt tgtgttgttc     4140

ggtgactcca tcacccagtt tagttgtacc cagtatggct ttcatccagc attacagaat     4200

gtgtatatcc gaaaattgga tgttattaac cgtggtttca gtggctacaa ctcagagcac     4260

gctagacaaa ttcttccaaa aattttagag tcggaaacca atatcaaatt gatgacaata     4320

ttttttggaa ctaacgatgc atacgactac atcaatgaaa tccagacagt cgagttagac     4380

agatataaag ataatttaag tgtaatggta cagatggtac tagacaaaaa tatcaaacca     4440

atcattattg gatccgaagt tcctattctc tagaaagtat aggaacttcc tcgagagtag     4500

tcgatgctgg gtattcgatt acatgtgtat aggaagattt tggtttttta ttcgttcttt     4560

tttttaattt ttgttaaatt agtttagaga tttcattaat acatagatgg gtgctatttc     4620

cgaaacttta cttctatccc ctgtatccct tattatccct ctcagtcaca tgattgctgt     4680

aattgtcgtg caggacacaa actccctaac ggacttaaac cataaacaag ctcagaacca     4740

taagccgaca tcactccttc ttctctcttc tccaaccaat agcatggaca gacccgagac     4800

g                                                                     4801


<210>  31
<211>  643
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis CYP52A12B

<400>  31
cgtctcatgg ccacacaaga aatcatcgat tctgtacttc cgtacttgac caaatggtac       60

actgtgatta ctgcagcagt attagtcttc cttatctcca caaacatcaa gaactacgtc      120

aaggcaaaga aattgaaatg tgtcgatcca ccatacttga aggatgccgg tctcactggt      180

attctgtctt tgatcgccgc catcaaggcc aagaacgacg gtagattggc taactttgcc      240

gatgaagttt tcgacgagta cccaaaccac accttctact tgtctgttgc cggtgctttg      300

aagtaggcgg ccgctagatc ttgcgaagct ccatctcgag gttgtctaca agacccaccg      360

tttggaagaa tactacggta aggacgctaa cgacttcaga ccagaaagat ggtttgaacc      420

atctactaag aagttgggct gggcttatgt tccattcaac ggtggtccaa gagtctgctt      480

gggtcaacaa ttcgccttga ctgaagcttc ttatgtgatc actagattgg cccagatgtt      540

tgaaactgtc tcatctgatc caggtctcga ataccctcca ccaaagtgta ttcacttgac      600

catgagtcac aacgatggtg tctttgtcaa gatgtaagag acg                        643


<210>  32
<211>  4795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis CYP52A12B

<400>  32
cgtctcatgg ccacacaaga aatcatcgat tctgtacttc cgtacttgac caaatggtac       60

actgtgatta ctgcagcagt attagtcttc cttatctcca caaacatcaa gaactacgtc      120

aaggcaaaga aattgaaatg tgtcgatcca ccatacttga aggatgccgg tctcactggt      180

attctgtctt tgatcgccgc catcaaggcc aagaacgacg gtagattggc taactttgcc      240

gatgaagttt tcgacgagta cccaaaccac accttctact tgtctgttgc cggtgctttg      300

aagtaggcgg ccgctctaga actagtggat ctgaagttcc tattctctag aaagtatagg      360

aacttcctgc aggaccacct ttgattgtaa atagtaataa ttaccaccct tatctaatta      420

tttatttaac ttatttattt atttattata catatataca aatctaataa agtgaaaatc      480

tcccccttca cacttcacat atgttaggcg tcatcctgtg ctcccgagaa ccagtaccag      540

tacatcgctg tttcgttcga gacttgaggt ctagttttat acgtgaagag gtcaatgccg      600

ccgagagtaa agccacattt tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgtc      660

tctaatcgta tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa ttcgatgaga      720

ctgtgcgcga ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc gatagaggct      780

agatcgttcc atgttgagtt gagttcaatc ttcccgacaa gctcttggtc gatgaatgcg      840

ccatagcaag cagagtcttc atcagagtca tcatccgaga tgtaatcctt ccggtagggg      900

ctcacacttc tggtagatag ttcaaagcct tggtcggata ggtgcacatc gaacacttca      960

cgaacaatga aatggttctc agcatccaat gtttccgcca cctgctcagg gatcaccgaa     1020

attttcatat gagaaccgtt atcgataact aaagcagcaa cttcttctat aaaaatgggt     1080

tagtatgaca gtcatttaaa taaggaattt ttcagttggc ttggtttcaa ttcaatgttc     1140

gttttttttt tttcttgctg tgtttgtgtt tgtgttgttt atagttgtgt gcactgagcg     1200

tcgaaaaaaa aaattcatag tgagccggga aatctgtata gcccagataa caacacaagt     1260

ccaaactaga aactcgtcaa acaccaaaag caatgttgaa tcaattgcct tgcacaagta     1320

cacgtaggaa aacataaaac attgcaattt tgaatattga gccttttgtc gtaacattga     1380

ttgataggat tactcaccga atggttttga aaccactgcc gacagatcaa tcaatcaatc     1440

aaaaaacgtg aactttgaaa aaggggaaga acagatacat tgaagttagc catttccatt     1500

gatcgtcaca acatatctga taaattactt tcaaaattat aagctgatgt gtgtgtatta     1560

ttaatgtgac agtaacatcc caaacgagaa atattatgtc gacaacaaaa aagtttgatc     1620

tgaattgaaa atgaagtttt cccaccctac ccatttgtca tattgaaacc aatcaactga     1680

ttaatcaatc aattagaatt gaagctaaac taaaacatac caccgtccat tttgaatgat     1740

tatatttttt taatattaat atcgagataa tgtttctaag aaagaaagaa aaccaggagt     1800

gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc tgaaaatata taaaaaaaaa     1860

ttgtttcgtt ggcaataaat cttggtgaga acagcgaccg aaagcaaata agaacaaaat     1920

atgagtgtat tacgttgaac aactaattaa cgtgtgtgta tggatctttt tttctttttt     1980

ctctttaacc gactataaac aacaaacatt tttgggcagt gcacacacta cttaatatac     2040

acagcataaa ttacacgatt agaaacaaat tagcttatta aaataaccta atcaaaccga     2100

atattttatg gtattatgag taaactatat aatataaata gcacacaccc acaacaacaa     2160

caaaggaaaa ctaaaaggtt ttttcttttt gaaaagatcg ttttctttat tattctctag     2220

ttttgacgct cgacatttta tgatggaatg aatgggatga atcatcaaac aagagaaaat     2280

acccgtgacg aaaataataa aataagttcc tctgatacag aagatgaaaa caacaacaac     2340

aagatataga aatgccttgg gtggctattt tatagtctta actttttaat gtatatttgt     2400

tttgtttttt tacataataa tactttataa aagctaagct aaattcaagt aaaatttcaa     2460

tctctcaaat aaaacatttt tctctttttc ttaaatttag ttttatatat ttataaaata     2520

tacaaagatt tttttaaaaa agtaacaagt tatatatgta ataacaaaaa gaagaataac     2580

aagaatacaa aaccagattt ccagatttcc agaatttcac tcttatatgc gtctatttat     2640

gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg     2700

tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt     2760

agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga     2820

aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc     2880

cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc     2940

cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt     3000

tatagcaaag attgaataag gcgcattttt cttcaaagct ttattgtacg atctgactaa     3060

gttatctttt aataattggt attcctgttt attgcttgaa gaattgccgg tcctatttac     3120

tcgttttagg actggttcag aattcctcaa aaattcatcc aaatatacaa gtggatcgat     3180

cctacccctt gcgctaaaga agtatatgtg cctactaacg cttgtctttg tctctgtcac     3240

taaacactgg attattactc ccaaatactt attttggact aatttaaatg atttcggatc     3300

aacgttctta atatcgctga atcttccaca attgatgaaa gtagctagga agaggaattg     3360

gtataaagtt tttgtttttg taaatctcga agtatactca aacgaattta gtattttctc     3420

agtgatctcc cagatgcttt caccctcact tagaagtgct ttaagcattt ttttactgtg     3480

gctatttccc ttatctgctt cttccgatga ttcgaactgt aattgcaaac tacttacaat     3540

atcagtgata tcagattgat gtttttgtcc atagtaagga ataattgtaa attcccaagc     3600

aggaatcaat ttctttaatg aggcttccaa aattgttgct ttttgcgtct tgtatttaaa     3660

ctggagtgat ttattgacaa tatcgaaact caacgaattg cttatgatag tattatagct     3720

catgaatgtg gctctcttga ttgctgttcc gttatgtgta atcatccaac ataaataggt     3780

tagttcagca gcacataatg ctattttctc acctgaaggt ctttcaaacc tttccacaaa     3840

ctgacgaaca agcaccttag gtggtgtttt acataatata tcaaattgtg gcatgtcgac     3900

gattattagt taaaccactg caaaaagttg gggaaaattt tgcccatttt tataccgtgt     3960

cttcgtctat cgcctccccc actccccaat ctttgaatta ttccgaaata ttcagcgaac     4020

ggggtgtaca caaaaactaa cattctcaac tgcataattt gaaaaatggc gtgggacaag     4080

aaaaaaaaaa aattctcaac catagcaatc atggaatacg gtaaatttgt gttgttcggt     4140

gactccatca cccagtttag ttgtacccag tatggctttc atccagcatt acagaatgtg     4200

tatatccgaa aattggatgt tattaaccgt ggtttcagtg gctacaactc agagcacgct     4260

agacaaattc ttccaaaaat tttagagtcg gaaaccaata tcaaattgat gacaatattt     4320

tttggaacta acgatgcata cgactacatc aatgaaatcc agacagtcga gttagacaga     4380

tataaagata atttaagtgt aatggtacag atggtactag acaaaaatat caaaccaatc     4440

attattggat ccgaagttcc tattctctag aaagtatagg aacttcctcg aggttgtcta     4500

caagacccac cgtttggaag aatactacgg taaggacgct aacgacttca gaccagaaag     4560

atggtttgaa ccatctacta agaagttggg ctgggcttat gttccattca acggtggtcc     4620

aagagtctgc ttgggtcaac aattcgcctt gactgaagct tcttatgtga tcactagatt     4680

ggcccagatg tttgaaactg tctcatctga tccaggtctc gaataccctc caccaaagtg     4740

tattcacttg accatgagtc acaacgatgg tgtctttgtc aagatgtaag agacg          4795


<210>  33
<211>  1305
<212>  DNA
<213>  Candida albicans

<400>  33
atgcaagcaa gcttattcag aattttcaga ggtgcgagtc tcaccactac cactgcagct       60

gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt gtctggatcc      120

actgtgctcc gaaaactgta taaaaggacc tattcatccc tggtcttatc ttctccagaa      180

ttattttttt ttcatcagtt taacaacaac aaacgttatt gtcatacaac aacaacaaca      240

aatacaaaaa caattatgtc tgaacaaatc ccaaaaactc aaaaagccgt tgtctttgat      300

accaatggtg gtcaattagt ctacaaggat tacccagttc caactccaaa gccaaatgaa      360

ttgttgattc acgtcaaata ctctggtgtc tgtcacactg atttacatgc ttggaaaggt      420

gactggccat tggctactaa attgccatta gttggtggtc acgaaggtgc cggtgtcgtt      480

gtcggtatgg gtgaaaacgt caaaggatgg aaaatcggtg actttgccgg tatcaaatgg      540

ttgaacggtt cttgtatgag ttgtgaattc tgtcaacaag gtgctgaacc aaactgtggt      600

gaagctgact tgtctggtta cactcacgat ggttcattcg aacaatacgc tactgctgat      660

gctgtccaag ccgctaaaat tccagctggt actgatttag ccaatgtcgc accaatctta      720

tgtgctggtg ttactgttta caaagcctta aagactgctg acttagcagc tggccaatgg      780

gttgctatct ccggtgctgg tggtggttta ggttctttgg ccgttcaata cgccagagcc      840

atgggtttga gagttgttgc tattgacggt ggtgacgaaa aaggtgaatt tgtcaaatca      900

ttgggtgctg aagcttacgt tgatttcacc aaagataaag atattgttga agctgttaag      960

aaggctactg atggtggtcc acacggtgct atcaatgtct ctgtttctga aaaagctatt     1020

gaccaatctg ttgaatatgt tagaccatta ggtaaagttg ttttggttgg tttaccagct     1080

cacgctaaag tcactgctcc agttttcgat gctgttgtca aatccattga aatcaaaggt     1140

tcttacgttg gtaacagaaa agatactgct gaagctattg acttcttctc cagaggttta     1200

atcaaatgcc caatcaagat tgtcggttta tctgacttgc cagaagtctt caaattgatg     1260

gaagaaggta aaatcttgag tagatacgta ttggacacca gttga                     1305


<210>  34
<211>  1308
<212>  DNA
<213>  Candida albicans

<400>  34
atggaagcaa ggtttttcag aattttcaag ggggggagtc tcaccactac cactgcagct       60

gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt gtctggatcc      120

actgtgctcc gaaaactgta taaaaggacc tattcatccc tggtcttatc ttctccagaa      180

ttattttttt ttcatcagtt taacaacaac aaacgttatt gtcatacaac aacaacaaca      240

aatacaaaaa caattatgtc tgaacaaatc ccaaaaactc aaaaagccgt tgtctttgat      300

accaatggtg gtcaattagt ctacaaggat tacccagttc caactccaaa gccaaatgaa      360

ttgttaatca acgtcaaata ctctggtgtc tgtcacactg atttacacgc ttggaaaggt      420

gactggccat tggctaccaa attgccatta gttggtggtc acgaaggtgc cggtgtcgtt      480

gtcggtatgg gtgaaaacgt caaaggatgg aaaatcggtg actttgccgg tatcaaatgg      540

ttgaacggtt cttgtatgag ctgtgaattc tgtcaacaag gtgctgaacc aaactgtggt      600

gaagctgact tgtctggtta cactcacgat ggttcattcg aacaatacgc tactgctgat      660

gctgtccaag ccgctaaaat tccagctggt actgatttag ccaatgtcgc accaatctta      720

tgtgctggtg ttactgttta caaagcctta aagactgctg acttagcagc tggccaatgg      780

gttgctatct ccggtgctgg tggtggttta ggttctttgg ccgttcaata cgccagagcc      840

atgggtttga gagttgttgc tattgacggt ggtgacgaaa aaggtgaatt tgttaaatca      900

ttgggtgctg aagcttacgt tgatttcacc aaagataaag atattgttga agctgtcaag      960

aaagctactg atggtggtcc acacggtgct atcaatgtct ctgtttctga aaaagccatt     1020

gaccaatctg ttgaatatgt tagaccatta ggtaaagttg ttttggttgg tttaccagct     1080

cacgctaaag tcactgctcc agttttcgat gctgttgtca aatccattga aatcaaaggt     1140

tcttacgttg gtaacagaaa agacactgct gaagctattg acttcttctc cagaggttta     1200

atcaaatgtc caatcaagat tgtcggttta tctgacttgc cagaagtctt caaattgatg     1260

gaagaaggta aaatcttggg tagatacgtc ttggacacca gtaaataa                  1308


<210>  35
<211>  1047
<212>  DNA
<213>  Candida albicans

<400>  35
atgtctgtcc caactactca aaaagctgtt attttcgaaa ccaacggtgg taagttggaa       60

tacaaagata tcccagtccc aaaaccaaaa gcaaatgaat tgttaatcaa cgttaaatac      120

tctggtgtct gtcacactga tttacacgct tggaaaggtg actggccatt ggctactaaa      180

ttgccattgg ttggtggtca cgaaggtgct ggtgttgttg tcgccttggg tgaaaacgtt      240

aaaggctgga aagttggtga ttacgctggt gttaaatggt tgaacggctc ttgtttgaac      300

tgtgaatact gtcaatcagg tgctgaacca aactgtgctg aagctgattt atccggttac      360

acccacgatg gttctttcca acaatatgct actgctgacg ctgttcaagc tgccagaatc      420

ccagctggta ccgacttagc caatgttgca ccaatcttat gtgctggtgt caccgtctac      480

aaagctttaa agactgctga attagaagcc ggtcaatggg ttgctatttc cggtgccgct      540

ggtggtttag gttctttagc tgttcaatac gccaaggcca tgggttacag agttcttgcc      600

atcgatggtg gtgaagacaa gggtgaattc gtcaaatcct tgggtgctga aacctttatt      660

gattttacca aagaaaaaga cgttgtcgaa gctgtcaaga aggccaccaa tggtggtcca      720

catggtgtta tcaatgtctc tgtctcagaa agagccattg gtcaatccac tgaatatgtc      780

agaactttag gtaaagttgt tttggttggt ttgccagctg gtgctaaaat tagtacccca      840

gtctttgatg ctgttatcaa gaccattcaa attaaaggtt cttatgtcgg taacagaaaa      900

gatactgctg aagccgttga tttcttcaca agaggtttga tcaaatgtcc aatcaagatt      960

gttggcttat ccgaattacc agaagtttac aaattgatgg aagaaggtaa aatcttgggt     1020

agatatgtct tggacaacga caaataa                                         1047


<210>  36
<211>  1047
<212>  DNA
<213>  Candida albicans

<400>  36
atgtctgtcc caactactca aaaagctgtt attttcgaaa ccaacggtgg taagttggaa       60

tacaaagata tcccagtccc aaaaccaaaa gcaaatgaat tgttaatcaa cgtcaaatac      120

tctggtgtct gtcacactga tttacacgct tggaaaggtg actggccatt ggctactaaa      180

ttgccattgg ttggtggtca cgaaggtgct ggtgttgttg tcgccttggg tgaaaacgtt      240

aaaggctgga aagttggtga ttacgctggt gttaaatggt tgaacggttc ttgtttgaac      300

tgtgaatact gtcaatcagg tgctgaacca aactgtgctg aagctgattt atccggttac      360

acccacgatg gttctttcca acaatatgct actgctgacg ctgttcaagc tgccagaatc      420

ccagctggta ccgacttagc caatgttgca ccaatcttat gtgctggtgt caccgtctac      480

aaagctttaa agactgctga attagaagcc ggtcaatggg ttgctatttc cggtgccgct      540

ggtggtttag gttctttagc tgttcaatac gccaaggcca tgggttacag agttcttgcc      600

atcgatggtg gtgaagacaa gggtgaattc gtcaaatcct tgggtgctga aacctttatt      660

gattttacca aagaaaaaga cgttgtcgaa gctgtcaaga aggccaccaa tggtggtcca      720

catggtgtta tcaatgtctc tgtctcagaa agagccattg gtcaatccac tgaatatgtc      780

agaactttag gtaaagttgt tttggttggt ttgccagctg gtgctaaaat tagtacccca      840

gtctttgatg ctgttatcaa gaccattcaa attaaaggtt cttatgtcgg taacagaaaa      900

gatactgctg aagccgttga tttcttcaca agaggtttga tcaaatgtcc aatcaagatt      960

gttggcttat ccgaattacc agaagtttac aaattgatgg aagaaggtaa aatcttgggt     1020

agatatgtct tggacaacga caaataa                                         1047


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Degenerate 5' oligonucleotide for PCR amplification of Candida 
       tropicalis alcohol dehydrogenase genes

<400>  37
actcaaaaag cygttrtytt ygawaccaay ggtgg                                  35


<210>  38
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Degenerate 3' oligonucleotide for PCR amplification of Candida 
       tropicalis alcohol dehydrogenase genes

<400>  38
gtccaakacr tatctacyca agattttacc ttcttc                                 36


<210>  39
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  39
gaattagaat acaaagatat cccagtgcca accccaaagg ccaacgaatt gctcatcaac       60

gtcaaatact cgggtgtctg ccacactgat ttgcacgcct ggaagggtga ctggccattg      120

gccaccaagt tgccattggt tggtggtcac gaaggtgctg gtgtcgttgt cggcatgggt      180

gaaaacgtca agggctggaa gattggtgac ttcgccggta tcaaatggtt gaacggttcc      240

tgtatgtcct gtgagttctg tcaacaaggt gctgaaccaa actgtggtga ggccgacttg      300

tctggttaca cccacgatgg ttctttcgaa caatacgcca ctgctgatgc tgttcaagcc      360

gccagaatcc cagctggtac tgatttggcc gaagttgccc caatcttgtg tgcgggtgtc      420

accgtctaca aagccttgaa gactgccgac ttggccgctg gtcaatgggt cgctatctcc      480

ggtgctggtg gtggtttggg ttccttggct gtccaatacg ccgtcgccat gggcttgaga      540

gtcgttgcca ttgacggtgg tgacgaaaag ggtgcctttg tcaagtcctt gggtgctgaa      600

gcctacattg atttcctcaa ggaaaaggac attgtctctg ctgtcaagaa ggccaccgat      660

ggaggtccac acggtgctat caatgtttcc gtttccgaaa aagccattga ccaatccgtc      720

gagtacgtta gaccattggg taaggttgtt ttggttggtt tgccagctgg ctccaaggtc      780

actgctggtg ttttcgaagc cgttgtcaag tccattgaaa tcaagggttc ctatgtcggt      840

aacagaaagg ataccgccga agccgttgac tttttctcca gaggcttgat caagtgtcca      900

atcaagattg ttggcttgag tgaattgcca caggtcttca agttgatg                   948


<210>  40
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  40
aagttagaat acaaagacgt gccggtccct gtccctaaac ccaacgaatt gcttgtcaac       60

gtcaagtact cgggtgtgtg tcattctgac ttgcatgtct ggaaaggcga ctggcccatt      120

cctgccaagt tgcccttggt gggaggtcac gaaggtgctg gtgtcgttgt cggcatgggt      180

gacaacgtca agggctggaa ggtgggggac ttggctggta tcaagtggtt gaatggttcg      240

tgtatgaact gtgagttttg ccaacagggc gcagaaccta actgttcaag agccgacatg      300

tctgggtata cccacgatgg aactttccaa caatacgcca ctgctgatgc tgtccaagct      360

gccaagatcc cagaaggcgc cgacatggct agtatcgccc cgatcttgtg cgctggtgtg      420

accgtgtaca aggctttgaa gaacgccgac ttgttggctg gccaatgggt ggctatctct      480

ggtgctggtg gtggtttggg ctccttgggt gtgcagtacg ctaaagccat gggttacaga      540

gtgttggcta tcgacggtgg tgacgagaga ggagagtttg tcaagtcctt gggcgccgaa      600

gtgtacattg acttccttaa ggaacaggac atcgttagtg ctatcagaaa ggcaactggt      660

ggtggtccac acggtgttat taacgtgtca gtgtccgaaa aggcaatcaa ccagtcggtg      720

gagtacgtca gaactttggg gaaagtggtt ttagttagct tgccggcagg tggtaaactc      780

actgctcctc ttttcgagtc tgttgctaga tcaatccaga ttagaactac gtgtgttggc      840

aacagaaagg atactactga agctattgat ttctttgtta gagggttgat cgattgccca      900

attaaagtcg ctggtttaag tgaagtgcca gagatttttg acttgatg                   948


<210>  41
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  41
ccattgcaat acatcgatat tccagtccca gtccctaagc caaacgattt gctcgtcaat       60

gtcaaatact ccggtctttg tcactcagat atacacctct ggaagggtga ctgattccca      120

gcatcaaaat tgccagttgt tggtggtcac gaaggtgcca gtgttgtcgt tgctattggt      180

gaaaacgtcc agggctggaa agtaggtgcc ttggcgggca taaagatgtt gaatggttcc      240

tgtatgaact gtgaattctg tcaacaaagt gcttaaccaa gctgtcccca tgctgatgtc      300

tcgggttact cccacgacgg cactttccaa cagtacgcta ccgctgatgc tgctcaagct      360

gctaaattcc cagctggttc tgatttagct agcatcgcac ctatatcctg tgccggtgtt      420

actgtttaca aagcattgaa gactgctggc ttgcatccgg gccaatgggt tgccatctcc      480

gatgctggtg gtggtttggg ttctttggcc gtgcaatacg ccaaggccat gggctacaga      540

gtggtggcca ttgactgcgg cggcgaaaat ggagtgtttg tcagatcgtt gggtactgaa      600

gctttcgttg attccaccaa ggaggccaat gtctctgagg ctatcatcaa ggctaccgac      660

ggtggtgtcc atggtgtcat caacgtttcc atttctgaaa aagccatcaa ccagtctgtt      720

gaaaatgtca gaactttggg tactgttgtc ttggttggtt tgccagctgg tgccaagctc      780

gaagcaccta tcttcaatgc cgttgccaaa tccatctaaa tcaaggattc ttacgtgggt      840

aaccgaagag acactgctga ggctgttgat ttcttcgcga aaggtttggt caagtgtcca      900

attaaggttg ttgagttgag tgaattgcca gagattttca aattgttg                   948


<210>  42
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  42
aaattagaat acaaggacat cccagttcca aagccaaagc caaacgaatt gctcatcaac       60

gtcaagtact ccggtgtctg ccacactgat ttacacgcct ggaagggtga ctggccattg      120

gacaccaagt tgccattggt gggtggtcac gaaggtgctg gtgttgttgt tgccattggt      180

gacaatgtca agggatggaa ggtcggtgat ttggccggtg tcaagtggtt gaacggttcc      240

tgtatgaact gtgagtactg tcaacagggt gccgaaccaa actgtccaca ggctgacttg      300

tctggttaca cccacgacgg ttctttccag caatacgcca ctgcagatgc cgtgcaagcc      360

gctagaattc cagctggtac tgatttagcc aacgttgccc ccatcttgtg tgctggtgtc      420

actgtttaca aggccttgaa gaccgccgac ttgcagccag gtcaatgggt cgccatttcc      480

ggtgccgctg gtggtttggg ttctttggcc gttcaatacg ccaaggccat gggctacaga      540

gttgtcgcca tcgatggtgg tgccgacaag ggtgagttcg tcaagtcttt gggcgctgag      600

gtctttgttg atttcctcaa ggaaaaggac attgttggtg ctgtcaagaa ggcaaccgat      660

ggtggcccac acggtgccgt taacgtttcc atctccgaaa aggccatcaa ccaatctgtc      720

gactacgtta gaaccttggg taaggttgtc ttggtcggtt tgccagctgg ctccaaggtt      780

tctgctccag tctttgactc cgtcgtcaag tccatccaaa tcaagggttc ctatgtcggt      840

aacagaaagg acactgccga agctgttgac tttttctcca gaggcttgat caagtgtcca      900

atcaaggttg tcggtttgag tgaattgcca gaagtctaca agttgatg                   948


<210>  43
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  43
ccattgcaat acaccgatat cccagttcca gtccctaagc caaacgaatt gctcgtccac       60

gtcaaatact ccggtgtttg tcactcagat atacacgtct ggaagggtga ctggttccca      120

gcatcgaaat tgcccgttgt tggtggtcac gaaggtgccg gtgttgtcgt tgccattggt      180

gaaaacgtcc aaggctggaa agtaggtgac ttggcaggta taaagatgtt gaatggttcc      240

tgtatgaact gtgaatactg tcaacaaggt gctgaaccaa actgtcccca cgctgatgtc      300

tcgggttact cccacgacgg tactttccaa cagtacgcta ccgccgatgc tgttcaagct      360

gctaaattcc cagctggttc tgatttagct agcatcgcac ctatatcctg cgccggtgtt      420

actgtttaca aagcattgaa aactgcaggc ttgcagccag gtcaatgggt tgccatctct      480

ggtgcagctg gtggtttggg ttctttggct gtgcaatacg ccaaggccat gggtttgaga      540

gtcgtggcca ttgacggtgg tgacgaaaga ggagtgtttg tcaaatcgtt gggtgctgaa      600

gttttcgttg atttcaccaa agaggccaat gtctctgagg ctatcatcaa ggctaccgac      660

ggtggtgccc atggcgtcat caacgtttcc atttctgaaa aagccatcaa ccagtctgtt      720

gaatatgtta gaactttggg aactgttgtc ttggttggtt tgccagctgg tgcaaagctc      780

gaagctccta tcttcaatgc cgttgccaaa tccatccaaa tcaaaggttc ttacgtggga      840

aacagaagag acactgctga ggctgttgat ttcttcgcta gaggtttggt caaatgtcca      900

attaaggttg ttgggttgag tgaattgcca gagattttca aattgttg                   948


<210>  44
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-A4

<400>  44
cgtctcaccc caaaggccaa cgaattgctc atcaacgtca aatactcggg tgtctgccac       60

actgatttgc acgcctggaa gggtgactgg ccattggcca ccaagttgcc attggttggt      120

ggtcacgaag gtgctggtgt cgttgtcggc atgggtgaaa acgtcaaggg ctggaagatt      180

ggtgacttcg ccggtatcaa atggtaggcg gccgctagat cttgcgaagc tccatctcga      240

ggagtacgtt agaccattgg gtaaggttgt tttggttggt ttgccagctg gctccaaggt      300

cactgctggt gttttcgaag ccgttgtcaa gtccattgaa atcaagggtt cctatgtcgg      360

taacagaaag gataccgccg aagccgttga ctttttctcc agaggcttga tcaagtgtcc      420

aatcaagatt gttggcttgg agacg                                            445


<210>  45
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-A4

<400>  45
cgtctcaccc caaaggccaa cgaattgctc atcaacgtca aatactcggg tgtctgccac       60

actgatttgc acgcctggaa gggtgactgg ccattggcca ccaagttgcc attggttggt      120

ggtcacgaag gtgctggtgt cgttgtcggc atgggtgaaa acgtcaaggg ctggaagatt      180

ggtgacttcg ccggtatcaa atggtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gaggagtacg ttagaccatt gggtaaggtt gttttggttg gtttgccagc     4440

tggctccaag gtcactgctg gtgttttcga agccgttgtc aagtccattg aaatcaaggg     4500

ttcctatgtc ggtaacagaa aggataccgc cgaagccgtt gactttttct ccagaggctt     4560

gatcaagtgt ccaatcaaga ttgttggctt ggagacg                              4597


<210>  46
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-A4B

<400>  46
cgtctctgtc aacaaggtgc tgaaccaaac tgtggtgagg ccgacttgtc tggttacacc       60

cacgatggtt ctttcgaaca atacgccact gctgatgctg ttcaagccgc cagaatccca      120

gctggtactg atttggccga agttgcccca atcttgtgtg cgggtgtcac cgtctacaaa      180

gccttgaaga ctgccgactt ggcctaggcg gccgctagat cttgcgaagc tccatctcga      240

gggtttgggt tccttggctg tccaatacgc cgtcgccatg ggcttgagag tcgttgccat      300

tgacggtggt gacgaaaagg gtgcctttgt caagtccttg ggtgctgaag cctacattga      360

tttcctcaag gaaaaggaca ttgtctctgc tgtcaagaag gccaccgatg gaggtccaca      420

cggtgctatc aatgtttccg agacg                                            445


<210>  47
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-A4B

<400>  47
cgtctctgtc aacaaggtgc tgaaccaaac tgtggtgagg ccgacttgtc tggttacacc       60

cacgatggtt ctttcgaaca atacgccact gctgatgctg ttcaagccgc cagaatccca      120

gctggtactg atttggccga agttgcccca atcttgtgtg cgggtgtcac cgtctacaaa      180

gccttgaaga ctgccgactt ggcctaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gagggtttgg gttccttggc tgtccaatac gccgtcgcca tgggcttgag     4440

agtcgttgcc attgacggtg gtgacgaaaa gggtgccttt gtcaagtcct tgggtgctga     4500

agcctacatt gatttcctca aggaaaagga cattgtctct gctgtcaaga aggccaccga     4560

tggaggtcca cacggtgcta tcaatgtttc cgagacg                              4597


<210>  48
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-B4

<400>  48
cgtctcaagc caaagccaaa cgaattgctc atcaacgtca agtactccgg tgtctgccac       60

actgatttac acgcctggaa gggtgactgg ccattggaca ccaagttgcc attggtgggt      120

ggtcacgaag gtgctggtgt tgttgttgcc attggtgaca atgtcaaggg atggaaggtc      180

ggtgatttgg ccggtgtcaa gtggtaggcg gccgctagat cttgcgaagc tccatctcga      240

ggactacgtt agaaccttgg gtaaggttgt cttggtcggt ttgccagctg gctccaaggt      300

ttctgctcca gtctttgact ccgtcgtcaa gtccatccaa atcaagggtt cctatgtcgg      360

taacagaaag gacactgccg aagctgttga ctttttctcc agaggcttga tcaagtgtcc      420

aatcaaggtt gtcggtttgg agacg                                            445


<210>  49
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-B4

<400>  49
cgtctcaagc caaagccaaa cgaattgctc atcaacgtca agtactccgg tgtctgccac       60

actgatttac acgcctggaa gggtgactgg ccattggaca ccaagttgcc attggtgggt      120

ggtcacgaag gtgctggtgt tgttgttgcc attggtgaca atgtcaaggg atggaaggtc      180

ggtgatttgg ccggtgtcaa gtggtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gaggactacg ttagaacctt gggtaaggtt gtcttggtcg gtttgccagc     4440

tggctccaag gtttctgctc cagtctttga ctccgtcgtc aagtccatcc aaatcaaggg     4500

ttcctatgtc ggtaacagaa aggacactgc cgaagctgtt gactttttct ccagaggctt     4560

gatcaagtgt ccaatcaagg ttgtcggttt ggagacg                              4597


<210>  50
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-B4B

<400>  50
cgtctctgtc aacagggtgc cgaaccaaac tgtccacagg ctgacttgtc tggttacacc       60

cacgacggtt ctttccagca atacgccact gcagatgccg tgcaagccgc tagaattcca      120

gctggtactg atttagccaa cgttgccccc atcttgtgtg ctggtgtcac tgtttacaag      180

gccttgaaga ccgccgactt gcagtaggcg gccgctagat cttgcgaagc tccatctcga      240

gggtttgggt tctttggccg ttcaatacgc caaggccatg ggctacagag ttgtcgccat      300

cgatggtggt gccgacaagg gtgagttcgt caagtctttg ggcgctgagg tctttgttga      360

tttcctcaag gaaaaggaca ttgttggtgc tgtcaagaag gcaaccgatg gtggcccaca      420

cggtgccgtt aacgtttccg agacg                                            445


<210>  51
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-B4B

<400>  51
cgtctctgtc aacagggtgc cgaaccaaac tgtccacagg ctgacttgtc tggttacacc       60

cacgacggtt ctttccagca atacgccact gcagatgccg tgcaagccgc tagaattcca      120

gctggtactg atttagccaa cgttgccccc atcttgtgtg ctggtgtcac tgtttacaag      180

gccttgaaga ccgccgactt gcagtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gagggtttgg gttctttggc cgttcaatac gccaaggcca tgggctacag     4440

agttgtcgcc atcgatggtg gtgccgacaa gggtgagttc gtcaagtctt tgggcgctga     4500

ggtctttgtt gatttcctca aggaaaagga cattgttggt gctgtcaaga aggcaaccga     4560

tggtggccca cacggtgccg ttaacgtttc cgagacg                              4597


<210>  52
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-A10

<400>  52
cgtctcgtcc ctaaacccaa cgaattgctt gtcaacgtca agtactcggg tgtgtgtcat       60

tctgacttgc atgtctggaa aggcgactgg cccattcctg ccaagttgcc cttggtggga      120

ggtcacgaag gtgctggtgt cgttgtcggc atgggtgaca acgtcaaggg ctggaaggtg      180

ggggacttgg ctggtatcaa gtggtaggcg gccgctagat cttgcgaagc tccatctcga      240

ggagtacgtc agaactttgg ggaaagtggt tttagttagc ttgccggcag gtggtaaact      300

cactgctcct cttttcgagt ctgttgctag atcaatccag attagaacta cgtgtgttgg      360

caacagaaag gatactactg aagctattga tttctttgtt agagggttga tcgattgccc      420

aattaaagtc gctggtttag agacg                                            445


<210>  53
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-A10

<400>  53
cgtctcgtcc ctaaacccaa cgaattgctt gtcaacgtca agtactcggg tgtgtgtcat       60

tctgacttgc atgtctggaa aggcgactgg cccattcctg ccaagttgcc cttggtggga      120

ggtcacgaag gtgctggtgt cgttgtcggc atgggtgaca acgtcaaggg ctggaaggtg      180

ggggacttgg ctggtatcaa gtggtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gaggagtacg tcagaacttt ggggaaagtg gttttagtta gcttgccggc     4440

aggtggtaaa ctcactgctc ctcttttcga gtctgttgct agatcaatcc agattagaac     4500

tacgtgtgtt ggcaacagaa aggatactac tgaagctatt gatttctttg ttagagggtt     4560

gatcgattgc ccaattaaag tcgctggttt agagacg                              4597


<210>  54
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-B11

<400>  54
cgtctcgtcc ctaagccaaa cgaattgctc gtccacgtca aatactccgg tgtttgtcac       60

tcagatatac acgtctggaa gggtgactgg ttcccagcat cgaaattgcc cgttgttggt      120

ggtcacgaag gtgccggtgt tgtcgttgcc attggtgaaa acgtccaagg ctggaaagta      180

ggtgacttgg caggtataaa gatgtaggcg gccgctagat cttgcgaagc tccatctcga      240

ggaatatgtt agaactttgg gaactgttgt cttggttggt ttgccagctg gtgcaaagct      300

cgaagctcct atcttcaatg ccgttgccaa atccatccaa atcaaaggtt cttacgtggg      360

aaacagaaga gacactgctg aggctgttga tttcttcgct agaggtttgg tcaaatgtcc      420

aattaaggtt gttgggttgg agacg                                            445


<210>  55
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-B11

<400>  55
cgtctcgtcc ctaagccaaa cgaattgctc gtccacgtca aatactccgg tgtttgtcac       60

tcagatatac acgtctggaa gggtgactgg ttcccagcat cgaaattgcc cgttgttggt      120

ggtcacgaag gtgccggtgt tgtcgttgcc attggtgaaa acgtccaagg ctggaaagta      180

ggtgacttgg caggtataaa gatgtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gaggaatatg ttagaacttt gggaactgtt gtcttggttg gtttgccagc     4440

tggtgcaaag ctcgaagctc ctatcttcaa tgccgttgcc aaatccatcc aaatcaaagg     4500

ttcttacgtg ggaaacagaa gagacactgc tgaggctgtt gatttcttcg ctagaggttt     4560

ggtcaaatgt ccaattaagg ttgttgggtt ggagacg                              4597


<210>  56
<211>  460
<212>  DNA
<213>  Candida tropicalis

<400>  56
tgaactgtga gttttgccaa cagggcgctg aacctaattg tccaagagcc gacatgtctg       60

gatataccca cgatgggact ttccaacaat atgctaccgc cgatgccgtc caagctgcca      120

agatcccaga aggcgcagac atggctagta tcgccccgat cttgtgtgct ggtgtgaccg      180

tgtacaaggc tttgaagaac gccgacttgt tggctggcca atgggtggct atctctggtg      240

ctggtggtgg tttgggctcc ttgggtgtgc agtacgctaa agccatgggt tacagagtgt      300

tagccatcga tggtggtgat gagagaggag agtttgtcaa gtcattgggc gccgaagtgt      360

acattgactt ccttaaggaa caggacattg ttagtgccat tagaaaggca actggtggtg      420

gcccacacgg tgttattaac gtctcggtgt ccgaaaaggc                            460


<210>  57
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-A10B

<400>  57
cgtctctgcc aacagggcgc tgaacctaat tgtccaagag ccgacatgtc tggatatacc       60

cacgatggga ctttccaaca atatgctacc gccgatgccg tccaagctgc caagatccca      120

gaaggcgcag acatggctag tatcgccccg atcttgtgtg ctggtgtgac cgtgtacaag      180

gctttgaaga acgccgactt gttgtaggcg gccgctagat cttgcgaagc tccatctcga      240

gggtttgggc tccttgggtg tgcagtacgc taaagccatg ggttacagag tgttagccat      300

cgatggtggt gatgagagag gagagtttgt caagtcattg ggcgccgaag tgtacattga      360

cttccttaag gaacaggaca ttgttagtgc cattagaaag gcaactggtg gtggcccaca      420

cggtgttatt aacgtgtcgg agacg                                            445


<210>  58
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-A10B

<400>  58
cgtctctgcc aacagggcgc tgaacctaat tgtccaagag ccgacatgtc tggatatacc       60

cacgatggga ctttccaaca atatgctacc gccgatgccg tccaagctgc caagatccca      120

gaaggcgcag acatggctag tatcgccccg atcttgtgtg ctggtgtgac cgtgtacaag      180

gctttgaaga acgccgactt gttgtaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gagggtttgg gctccttggg tgtgcagtac gctaaagcca tgggttacag     4440

agtgttagcc atcgatggtg gtgatgagag aggagagttt gtcaagtcat tgggcgccga     4500

agtgtacatt gacttcctta aggaacagga cattgttagt gccattagaa aggcaactgg     4560

tggtggccca cacggtgtta ttaacgtgtc ggagacg                              4597


<210>  59
<211>  948
<212>  DNA
<213>  Candida tropicalis

<400>  59
ccattgcaat acaccgatat cccagttcca gtccctaagc caaacgattt gctcgtcaat       60

gtcaaatact ccggtctttg tcactcagat atacacctct ggaagggtga ctgattccca      120

gcatcaaaat tgccagttgt tggtggtcac gaaggtgcca gtgttgtcgt tgctattggt      180

gaaaacgtcc agggctggaa agtaggtgcc ttggcgggca taaagatgtt gaatggttcc      240

tgtatgaact gtgaattctg tcaacaaagt gcttaaccaa gctgtcccca tgctgatgtc      300

tcgggttact cccacgacgg cactttccaa cagtacgcta ccgctgatgc tgctcaagct      360

gctaaattcc cagctggttc tgatttagct agcatcgcac ctatatcctg tgccggtgtt      420

actgtttaca aagcattgaa gactgctggc ttgcatccgg gccaatgggt tgccatctcc      480

gatgctggtg gtggtttggg ttctttggcc gtgcaatacg ccaaggccat gggctacaga      540

gtggtggcca ttgactgcgg cggcgaaaat ggagtgtttg tcagatcgtt gggtactgaa      600

gctttcgttg attccaccaa ggaggccaat gtctctgagg ctatcatcaa ggctaccgac      660

ggtggtgtcc atggtgtcat caacgtttcc atttctgaaa aagccatcaa ccagtctgtt      720

gaaaatgtca gaactttggg tactgttgtc ttggttggtt tgccagctgg tgccaagctc      780

gaagcaccta tcttcaatgc cgttgccaaa tccatctaaa tcaaggattc ttacgtgggt      840

aaccgaagag acactgctga ggctgttgat ttcttcgcga aaggtttggt caagtgtcca      900

attaaggttg ttgagttgag tgaattgcca gagattttca aattgttg                   948


<210>  60
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for Candida tropicalis ADH-B11B

<400>  60
cgtctctgtc aacaaagtgc ttaaccaagc tgtccccatg ctgatgtctc gggttactcc       60

cacgacggca ctttccaaca gtacgctacc gctgatgctg ctcaagctgc taaattccca      120

gctggttctg atttagctag catcgcacct atatcctgtg ccggtgttac tgtttacaaa      180

gcattgaaga ctgctggctt gcattaggcg gccgctagat cttgcgaagc tccatctcga      240

gggtttgggt tctttggccg tgcaatacgc caaggccatg ggctacagag tggtggccat      300

tgactgcggc ggcgaaaatg gagtgtttgt cagatcgttg ggtactgaag ctttcgttga      360

ttccaccaag gaggccaatg tctctgaggc tatcatcaag gctaccgacg gtggtgtcca      420

tggtgtcatc aacgtttccg agacg                                            445


<210>  61
<211>  4597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting construct for deletion of Candida tropicalis ADH-B11B

<400>  61
cgtctctgtc aacaaagtgc ttaaccaagc tgtccccatg ctgatgtctc gggttactcc       60

cacgacggca ctttccaaca gtacgctacc gctgatgctg ctcaagctgc taaattccca      120

gctggttctg atttagctag catcgcacct atatcctgtg ccggtgttac tgtttacaaa      180

gcattgaaga ctgctggctt gcattaggcg gccgctctag aactagtgga tctgaagttc      240

ctattctcta gaaagtatag gaacttcctg caggaccacc tttgattgta aatagtaata      300

attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac      360

aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt      420

gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta      480

tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca      540

ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac      600

tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca      660

acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca      720

agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag      780

atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat      840

aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc      900

acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca      960

acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg     1020

cttggtttca attcaatgtt cgtttttttt ttttcttgct gtgtttgtgt ttgtgttgtt     1080

tatagttgtg tgcactgagc gtcgaaaaaa aaaattcata gtgagccggg aaatctgtat     1140

agcccagata acaacacaag tccaaactag aaactcgtca aacaccaaaa gcaatgttga     1200

atcaattgcc ttgcacaagt acacgtagga aaacataaaa cattgcaatt ttgaatattg     1260

agccttttgt cgtaacattg attgatagga ttactcaccg aatggttttg aaaccactgc     1320

cgacagatca atcaatcaat caaaaaacgt gaactttgaa aaaggggaag aacagataca     1380

ttgaagttag ccatttccat tgatcgtcac aacatatctg ataaattact ttcaaaatta     1440

taagctgatg tgtgtgtatt attaatgtga cagtaacatc ccaaacgaga aatattatgt     1500

cgacaacaaa aaagtttgat ctgaattgaa aatgaagttt tcccacccta cccatttgtc     1560

atattgaaac caatcaactg attaatcaat caattagaat tgaagctaaa ctaaaacata     1620

ccaccgtcca ttttgaatga ttatattttt ttaatattaa tatcgagata atgtttctaa     1680

gaaagaaaga aaaccaggag tgaaaattag aaaaggaaag gaaaggaaaa aaagaaaaat     1740

ctgaaaatat ataaaaaaaa attgtttcgt tggcaataaa tcttggtgag aacagcgacc     1800

gaaagcaaat aagaacaaaa tatgagtgta ttacgttgaa caactaatta acgtgtgtgt     1860

atggatcttt ttttcttttt tctctttaac cgactataaa caacaaacat ttttgggcag     1920

tgcacacact acttaatata cacagcataa attacacgat tagaaacaaa ttagcttatt     1980

aaaataacct aatcaaaccg aatattttat ggtattatga gtaaactata taatataaat     2040

agcacacacc cacaacaaca acaaaggaaa actaaaaggt tttttctttt tgaaaagatc     2100

gttttcttta ttattctcta gttttgacgc tcgacatttt atgatggaat gaatgggatg     2160

aatcatcaaa caagagaaaa tacccgtgac gaaaataata aaataagttc ctctgataca     2220

gaagatgaaa acaacaacaa caagatatag aaatgccttg ggtggctatt ttatagtctt     2280

aactttttaa tgtatatttg ttttgttttt ttacataata atactttata aaagctaagc     2340

taaattcaag taaaatttca atctctcaaa taaaacattt ttctcttttt cttaaattta     2400

gttttatata tttataaaat atacaaagat ttttttaaaa aagtaacaag ttatatatgt     2460

aataacaaaa agaagaataa caagaataca aaaccagatt tccagatttc cagaatttca     2520

ctcttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat     2580

cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg     2640

ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg     2700

atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg     2760

atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac     2820

attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat     2880

gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc     2940

tttattgtac gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga     3000

agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc     3060

caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac     3120

gcttgtcttt gtctctgtca ctaaacactg gattattact cccaaatact tattttggac     3180

taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa     3240

agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc     3300

aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc     3360

tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg     3420

taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg     3480

aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca aaattgttgc     3540

tttttgcgtc ttgtatttaa actggagtga tttattgaca atatcgaaac tcaacgaatt     3600

gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt     3660

aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg     3720

tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat     3780

atcaaattgt ggcatgtcga cgattattag ttaaaccact gcaaaaagtt ggggaaaatt     3840

ttgcccattt ttataccgtg tcttcgtcta tcgcctcccc cactccccaa tctttgaatt     3900

attccgaaat attcagcgaa cggggtgtac acaaaaacta acattctcaa ctgcataatt     3960

tgaaaaatgg cgtgggacaa gaaaaaaaaa aaattctcaa ccatagcaat catggaatac     4020

ggtaaatttg tgttgttcgg tgactccatc acccagttta gttgtaccca gtatggcttt     4080

catccagcat tacagaatgt gtatatccga aaattggatg ttattaaccg tggtttcagt     4140

ggctacaact cagagcacgc tagacaaatt cttccaaaaa ttttagagtc ggaaaccaat     4200

atcaaattga tgacaatatt ttttggaact aacgatgcat acgactacat caatgaaatc     4260

cagacagtcg agttagacag atataaagat aatttaagtg taatggtaca gatggtacta     4320

gacaaaaata tcaaaccaat cattattgga tccgaagttc ctattctcta gaaagtatag     4380

gaacttcctc gagggtttgg gttctttggc cgtgcaatac gccaaggcca tgggctacag     4440

agtggtggcc attgactgcg gcggcgaaaa tggagtgttt gtcagatcgt tgggtactga     4500

agctttcgtt gattccacca aggaggccaa tgtctctgag gctatcatca aggctaccga     4560

cggtggtgtc catggtgtca tcaacgtttc cgagacg                              4597


<210>  62
<211>  1484
<212>  DNA
<213>  Candida tropicalis

<400>  62
tattaggcga agaggcatct agtagtagtg gcagtggtga gaacgtgggc gctgctatag       60

tgaacaatct ccagtcgatg gttaagaaga agagtgacaa accagcagtg aatgacttgt      120

ctgggtccgt gaggaaaaga aagaagcccg acacaaagga cagtaacgtc aagaaaccca      180

agaaataggg gggacctgtt tagatgtata ggaataaaaa ctccgagatg atctcaatgt      240

gtaatggagt tgtaatattg caaaggggga aaatcaagac tcaaacgtgt gtatgagtga      300

gcgtacgtat atctccgaga gtagtatgac ataatgatga ctgtgaatca tcgtaatctc      360

acacaaaaac cccattgtcg gccatatacc acaccaagca acaccacata tcccccggaa      420

aaaaaaacgt gaaaaaaaga aacaatcaaa actacaacct actccttgat cacacagtca      480

ttgatcaagt tacagttcct gctagggaat gaccaaggta caaatcagca ccttaatggt      540

tagcacgctc tcttactctc tctcacagtc ttccggcccc tattcaaaat tctgcacttc      600

catttgaccc cagggttggg aaacagggcc acaaaagaaa aacccgacgt gaatgaaaaa      660

actaagaaaa gaaaaaaaat tatcacacca gaaatttacc taattgggta attcccatcg      720

gtgtttttcc tggattgtcg cacgcacgca tgctgaaaaa agtgttcgag ttttgctttt      780

gcctcggagt ttcacgcaag tttttcgatc tcggaaccgg agggcggtcg ccttgttgtt      840

tgtgatgtcg tgctttgggt gttctaatgt gctgttattg tgctcttttt ttttcttctt      900

tttttggtga tcatatgata ttgctcggta gattactttc gtgtgtaggt attcttttag      960

acgtttggtt attgggtaga tatgagagag agagagtggg tgggggagga gttggttgta     1020

ggagggaccc ctgggaggaa gtgtagttga gttttccctg acgaatgaaa atacgttttt     1080

gagaagataa tacaggaaag gtgtgtcggt gaatttccat ctatccgagg atatgagtgg     1140

aggagagtcg tgtgcgtgtg gttaatttag gatcagtgga acacacaaag taactaagac     1200

agagagacag agagaaaaat ctggggaaga gacaaagagt cagagtgtgt gagttattct     1260

gtattgtgaa atttttttgc ccaactacat aatattgctg aaactaattt tacttaaaaa     1320

gaaaagccaa caacgtcccc agtaaaactt ttctataaat atcagcagtt ttccctttcc     1380

tccattcctc ttcttgtctt ttttcttact ttcccttttt tatacctttt cattatcatc     1440

ctttataatt gtctaaccaa caactatata tctatcaacc atgg                      1484


<210>  63
<211>  319
<212>  DNA
<213>  Candida tropicalis

<400>  63
aagaaaaaag aaaaggtaaa gaacttcatt tgagatgaac ttttgtatat gacttttagt       60

ttctactttt ttttttattt attgcttaat tttctttatt tcaatccccc atagtttgtg      120

tagaatatat ttattcattc tggtaactca aacacgtagc aagctcgttg catctcgcct      180

cgtcacgggt acagctctgg aaccaaagac aaaaaaaaaa gttgatccga accctctcgc      240

tattccttgc tatgctatcc acgagatggg gtttatcagc ccaggcaagt cactaaagag      300

acaaagaccc agaaagaat                                                   319


<210>  64
<211>  412
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  64
cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg       60

actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa attttccctc      120

tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga      180

ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt      240

tcttgaaatt ttttttttta gtttttttct ctttcagtga cctccattga tatttaagtt      300

aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt      360

ttttacttct tgttcattag aaagaaagca tagcaatcta atctaagggg cg              412


<210>  65
<211>  68
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EM7 promoter

<400>  65
gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga       60

actaaacc                                                                68


<210>  66
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zeocin-resistance gene optimized for Candida tropicali

<400>  66
atgtctaaat taacctctgc tgttccagtg ttaaccgccc gtgatgttgc cggtgcagtg       60

gaattttgga ctgaccgttt gggtttctca cgtgactttg tcgaagatga ttttgctggc      120

gttgtgcgtg atgacgtcac tttgttcatc tctgctgttc aggatcaggt cgtcccagac      180

aacactttgg cctgggtctg ggttcgtggt ttggacgaat tgtacgctga gtggagtgaa      240

gttgtgtcta caaactttcg tgatgcatca ggtccagcta tgaccgaaat tggcgaacaa      300

ccttggggcc gtgagttcgc tttacgtgat ccagccggta attgcgtgca cttcgttgct      360

gaggagcaag attag                                                       375


<210>  67
<211>  230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYC1 transcription terminator

<400>  67
cacgtccgac ggcggcccac gggtcccagg cctcggagat ccgtccccct tttcctttgt       60

cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct      120

aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt      180

tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc                 230


<210>  68
<211>  805
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC bacterial origin of replication

<400>  68
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc       60

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct      120

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa      180

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag      240

tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc      300

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg      360

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca      420

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat      480

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg      540

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc      600

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc      660

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc      720

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg      780

cctttgagtg agctgatacc gctcg                                            805


<210>  69
<211>  1554
<212>  DNA
<213>  Candida tropicalis

<400>  69
atggctgagc aattgttgga atattggtac gttgtggtgc cagttttgta tatcattaag       60

caattattgg cctacaccaa gaccagagtc ttaatgaaga agttgggtgc cgccccagtt      120

actaacaaat tgtacgacaa cgctttcgga attgtcaacg gatggaaggc tttgcaattt      180

aagaaggagg gtagagccca agaatacaat gattataagt ttgatcacag taaaaaccct      240

tccgtcggta cttacgtgtc cattttgttt ggcactagaa ttgtcgtcac caaagaccca      300

gaaaatatca aagctatctt ggctacacaa tttggtgact tctccttggg caaaagacac      360

accttattta agccattgtt gggagatggt attttcacct tagacggcga aggttggaaa      420

cactccagag ccatgttgcg tccacaattt gcaagagaac aggttgctca cgttacctct      480

ttggaaccac attttcaatt attgaagaaa cacattttga aacacaaggg tgagtacttc      540

gacatccaag aattattctt tagattcacc gttgactccg ccactgagtt tttgtttggt      600

gagtccgttc attccttgaa agacgaaagt attggtatta accaagacga catcgacttc      660

gctggaagaa aggacttcgc tgaatccttt aataaggccc aggaatactt ggctatcaga      720

actttggtcc agacctttta ctggttggtg aacaataagg aatttagaga ctgtaccaaa      780

agtgtccata aattcaccaa ttattacgtc caaaaggcct tagacgcttc accagaggaa      840

ttagaaaagc aatccggtta cgtgttctta tatgagttgg tgaagcaaac acgtgatcca      900

aacgtcttga gagatcaaag tttaaacatt ttattggctg gtagagatac tactgctggc      960

ttgttgtctt ttgctgtttt tgaattggcc agacatccag agatttgggc taaattgaga     1020

gaagaaattg agcaacagtt cggtttgggt gaagattctc gtgttgagga gatcactttc     1080

gaatcattga agagatgtga atatttgaag gctttcttga acgaaacctt gagaatttat     1140

ccatctgttc caagaaactt tagaatcgca accaaaaaca caacattgcc aagaggaggt     1200

ggaagtgatg gcacctctcc aattttgatt caaaaaggtg aagctgtctc ttatggtatt     1260

aattccactc acttggaccc agtttattat ggtccagatg ccgccgaatt tagaccagaa     1320

agatggtttg aaccatctac caagaagttg ggatgggctt atttgccatt caacggtggt     1380

cctagaatct gcttgggtca acaattcgct ttgaccgaag ctggctacgt tttggttaga     1440

ttggtccagg agttctcaca cgtgagatcc gacccagacg aggtttatcc accaaagaga     1500

ttaactaact tgaccatgtg tttgcaagac ggtgctattg ttaagttcga ctag           1554


<210>  70
<211>  5268
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic integration vector for integrating CYP52A17 under control
       of the isocitrate lyase promoter

<400>  70
agatctgaat tctattaggc gaagaggcat ctagtagtag tggcagtggt gagaacgtgg       60

gcgctgctat agtgaacaat ctccagtcga tggttaagaa gaagagtgac aaaccagcag      120

tgaatgactt gtctgggtcc gtgaggaaaa gaaagaagcc cgacacaaag gacagtaacg      180

tcaagaaacc caagaaatag gggggacctg tttagatgta taggaataaa aactccgaga      240

tgatctcaat gtgtaatgga gttgtaatat tgcaaagggg gaaaatcaag actcaaacgt      300

gtgtatgagt gagcgtacgt atatctccga gagtagtatg acataatgat gactgtgaat      360

catcgtaatc tcacacaaaa accccattgt cggccatata ccacaccaag caacaccaca      420

tatcccccgg aaaaaaaaac gtgaaaaaaa gaaacaatca aaactacaac ctactccttg      480

atcacacagt cattgatcaa gttacagttc ctgctaggga atgaccaagg tacaaatcag      540

caccttaatg gttagcacgc tctcttactc tctctcacag tcttccggcc cctattcaaa      600

attctgcact tccatttgac cccagggttg ggaaacaggg ccacaaaaga aaaacccgac      660

gtgaatgaaa aaactaagaa aagaaaaaaa attatcacac cagaaattta cctaattggg      720

taattcccat cggtgttttt cctggattgt cgcacgcacg catgctgaaa aaagtgttcg      780

agttttgctt ttgcctcgga gtttcacgca agtttttcga tctcggaacc ggagggcggt      840

cgccttgttg tttgtgatgt cgtgctttgg gtgttctaat gtgctgttat tgtgctcttt      900

ttttttcttc tttttttggt gatcatatga tattgctcgg tagattactt tcgtgtgtag      960

gtattctttt agacgtttgg ttattgggta gatatgagag agagagagtg ggtgggggag     1020

gagttggttg taggagggac ccctgggagg aagtgtagtt gagttttccc tgacgaatga     1080

aaatacgttt ttgagaagat aatacaggaa aggtgtgtcg gtgaatttcc atctatccga     1140

ggatatgagt ggaggagagt cgtgtgcgtg tggttaattt aggatcagtg gaacacacaa     1200

agtaactaag acagagagac agagagaaaa atctggggaa gagacaaaga gtcagagtgt     1260

gtgagttatt ctgtattgtg aaattttttt gcccaactac ataatattgc tgaaactaat     1320

tttacttaaa aagaaaagcc aacaacgtcc ccagtaaaac ttttctataa atatcagcag     1380

ttttcccttt cctccattcc tcttcttgtc ttttttctta ctttcccttt tttatacctt     1440

ttcattatca tcctttataa ttgtctaacc aacaactata tatctatcaa ccatggctga     1500

gcaattgttg gaatattggt acgttgtggt gccagttttg tatatcatta agcaattatt     1560

ggcctacacc aagaccagag tcttaatgaa gaagttgggt gccgccccag ttactaacaa     1620

attgtacgac aacgctttcg gaattgtcaa cggatggaag gctttgcaat ttaagaagga     1680

gggtagagcc caagaataca atgattataa gtttgatcac agtaaaaacc cttccgtcgg     1740

tacttacgtg tccattttgt ttggcactag aattgtcgtc accaaagacc cagaaaatat     1800

caaagctatc ttggctacac aatttggtga cttctccttg ggcaaaagac acaccttatt     1860

taagccattg ttgggagatg gtattttcac cttagacggc gaaggttgga aacactccag     1920

agccatgttg cgtccacaat ttgcaagaga acaggttgct cacgttacct ctttggaacc     1980

acattttcaa ttattgaaga aacacatttt gaaacacaag ggtgagtact tcgacatcca     2040

agaattattc tttagattca ccgttgactc cgccactgag tttttgtttg gtgagtccgt     2100

tcattccttg aaagacgaaa gtattggtat taaccaagac gacatcgact tcgctggaag     2160

aaaggacttc gctgaatcct ttaataaggc ccaggaatac ttggctatca gaactttggt     2220

ccagaccttt tactggttgg tgaacaataa ggaatttaga gactgtacca aaagtgtcca     2280

taaattcacc aattattacg tccaaaaggc cttagacgct tcaccagagg aattagaaaa     2340

gcaatccggt tacgtgttct tatatgagtt ggtgaagcaa acacgtgatc caaacgtctt     2400

gagagatcaa agtttaaaca ttttattggc tggtagagat actactgctg gcttgttgtc     2460

ttttgctgtt tttgaattgg ccagacatcc agagatttgg gctaaattga gagaagaaat     2520

tgagcaacag ttcggtttgg gtgaagattc tcgtgttgag gagatcactt tcgaatcatt     2580

gaagagatgt gaatatttga aggctttctt gaacgaaacc ttgagaattt atccatctgt     2640

tccaagaaac tttagaatcg caaccaaaaa cacaacattg ccaagaggag gtggaagtga     2700

tggcacctct ccaattttga ttcaaaaagg tgaagctgtc tcttatggta ttaattccac     2760

tcacttggac ccagtttatt atggtccaga tgccgccgaa tttagaccag aaagatggtt     2820

tgaaccatct accaagaagt tgggatgggc ttatttgcca ttcaacggtg gtcctagaat     2880

ctgcttgggt caacaattcg ctttgaccga agctggctac gttttggtta gattggtcca     2940

ggagttctca cacgtgagat ccgacccaga cgaggtttat ccaccaaaga gattaactaa     3000

cttgaccatg tgtttgcaag acggtgctat tgttaagttc gactaggcgg ccgcaagaaa     3060

aaagaaaagg taaagaactt catttgagat gaacttttgt atatgacttt tagtttctac     3120

tttttttttt atttattgct taattttctt tatttcaatc ccccatagtt tgtgtagaat     3180

atatttattc attctggtaa ctcaaacacg tagcaagctc gttgcatctc gcctcgtcac     3240

gggtacagct ctggaaccaa agacaaaaaa aaaagttgat ccgaaccctc tcgctattcc     3300

ttgctatgct atccacgaga tggggtttat cagcccaggc aagtcactaa aggatccccc     3360

acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact     3420

ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt ttccctcttt     3480

cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagagaccg     3540

cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct     3600

tgaaattttt ttttttagtt tttttctctt tcagtgacct ccattgatat ttaagttaat     3660

aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta caactttttt     3720

tacttcttgt tcattagaaa gaaagcatag caatctaatc taaggggcgg tgttgacaat     3780

taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg     3840

tctaaattaa cctctgctgt tccagtgtta accgcccgtg atgttgccgg tgcagtggaa     3900

ttttggactg accgtttggg tttctcacgt gactttgtcg aagatgattt tgctggcgtt     3960

gtgcgtgatg acgtcacttt gttcatctct gctgttcagg atcaggtcgt cccagacaac     4020

actttggcct gggtctgggt tcgtggtttg gacgaattgt acgctgagtg gagtgaagtt     4080

gtgtctacaa actttcgtga tgcatcaggt ccagctatga ccgaaattgg cgaacaacct     4140

tggggccgtg agttcgcttt acgtgatcca gccggtaatt gcgtgcactt cgttgctgag     4200

gagcaagatt agcacgtccg acggcggccc acgggtccca ggcctcggag atccgtcccc     4260

cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctccccc     4320

cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta     4380

tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt     4440

tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt     4500

gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa aaggccagca     4560

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     4620

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     4680

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     4740

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc     4800

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     4860

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     4920

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     4980

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     5040

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     5100

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     5160

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     5220

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagatc                  5268


<210>  71
<211>  1569
<212>  DNA
<213>  Candida tropicalis

<400>  71
atgactgttc acgacattat tgctacctac tttactaagt ggtacgtcat tgttccattg       60

gccttaatcg cctatagagt tttggattac ttttacggta gatacttgat gtacaaattg      120

ggtgctaaac cattctttca gaaacaaact gacggttgtt ttggttttaa ggccccttta      180

gaattattga agaaaaagtc tgacggtaca ttgatcgatt ttaccttgca aagaatccac      240

gacttggaca gaccagatat cccaaccttt acctttcctg ttttctcaat caatttggtg      300

aatactttgg aacctgaaaa catcaaggct atcttggcta cccagttcaa tgattttagt      360

ttgggcacca gacactcaca ttttgcacca ttgttgggag atggtatctt tacattggat      420

ggtgctggtt ggaagcattc aagatccatg ttaagaccac aatttgcaag agagcagatt      480

tcccatgtta agttgttgga accacatgtt caagtcttct tcaagcatgt tagaaaagcc      540

caaggtaaga cttttgatat tcaggagttg ttctttagat tgactgttga ttctgccacc      600

gaatttttgt ttggtgaatc cgtcgaatcc ttgagagacg aatctattgg tatgtctatt      660

aacgctttgg attttgatgg taaggctgga tttgcagatg catttaacta ttcccaaaac      720

tatttagctt ctagagctgt catgcaacaa ttatactggg ttttaaatgg taaaaagttc      780

aaggaatgta atgctaaggt ccacaagttc gctgactatt atgtcaacaa ggctttagac      840

ttaactccag agcaattaga aaagcaggat ggttacgtct tcttatatga attggtcaaa      900

cagactagag ataagcaagt tttgagagat cagttattga atatcatggt cgctggtaga      960

gacacaactg ctggtttgtt gtcctttgtc ttctttgaat tggccagaaa cccagaagtc     1020

accaacaaat taagagagga aatcgaagat aaatttggat taggcgaaaa tgcaagtgtc     1080

gaggacattt cctttgagtc tttgaagtct tgtgaatatt tgaaagctgt tttgaacgag     1140

acattgcgtt tgtacccttc agttccacag aactttagag ttgcaaccaa gaacaccaca     1200

ttgcctagag gcggtggtaa agatggtttg tctcctgttt tggtgagaaa gggtcaaacc     1260

gttatttacg gtgtctacgc cgcccacaga aacccagcag tctatggtaa agatgctttg     1320

gaattcagac cagagcgttg gttcgaacct gaaaccaaaa agttgggttg ggccttctta     1380

cctttcaacg gaggtccaag aatctgtttg ggtcagcaat tcgccttgac agaagcctca     1440

tatgtgactg tgagattgtt gcaagaattc gctcacttgt ctatggaccc tgacactgaa     1500

taccctccta aaaagatgtc ccatttgacc atgtcattat ttgacggtgc taatattgaa     1560

atgtattag                                                             1569


<210>  72
<211>  5283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic integration vector for integrating CYP52A13 under control
       of the isocitrate lyase promoter

<400>  72
agatctgaat tctattaggc gaagaggcat ctagtagtag tggcagtggt gagaacgtgg       60

gcgctgctat agtgaacaat ctccagtcga tggttaagaa gaagagtgac aaaccagcag      120

tgaatgactt gtctgggtcc gtgaggaaaa gaaagaagcc cgacacaaag gacagtaacg      180

tcaagaaacc caagaaatag gggggacctg tttagatgta taggaataaa aactccgaga      240

tgatctcaat gtgtaatgga gttgtaatat tgcaaagggg gaaaatcaag actcaaacgt      300

gtgtatgagt gagcgtacgt atatctccga gagtagtatg acataatgat gactgtgaat      360

catcgtaatc tcacacaaaa accccattgt cggccatata ccacaccaag caacaccaca      420

tatcccccgg aaaaaaaaac gtgaaaaaaa gaaacaatca aaactacaac ctactccttg      480

atcacacagt cattgatcaa gttacagttc ctgctaggga atgaccaagg tacaaatcag      540

caccttaatg gttagcacgc tctcttactc tctctcacag tcttccggcc cctattcaaa      600

attctgcact tccatttgac cccagggttg ggaaacaggg ccacaaaaga aaaacccgac      660

gtgaatgaaa aaactaagaa aagaaaaaaa attatcacac cagaaattta cctaattggg      720

taattcccat cggtgttttt cctggattgt cgcacgcacg catgctgaaa aaagtgttcg      780

agttttgctt ttgcctcgga gtttcacgca agtttttcga tctcggaacc ggagggcggt      840

cgccttgttg tttgtgatgt cgtgctttgg gtgttctaat gtgctgttat tgtgctcttt      900

ttttttcttc tttttttggt gatcatatga tattgctcgg tagattactt tcgtgtgtag      960

gtattctttt agacgtttgg ttattgggta gatatgagag agagagagtg ggtgggggag     1020

gagttggttg taggagggac ccctgggagg aagtgtagtt gagttttccc tgacgaatga     1080

aaatacgttt ttgagaagat aatacaggaa aggtgtgtcg gtgaatttcc atctatccga     1140

ggatatgagt ggaggagagt cgtgtgcgtg tggttaattt aggatcagtg gaacacacaa     1200

agtaactaag acagagagac agagagaaaa atctggggaa gagacaaaga gtcagagtgt     1260

gtgagttatt ctgtattgtg aaattttttt gcccaactac ataatattgc tgaaactaat     1320

tttacttaaa aagaaaagcc aacaacgtcc ccagtaaaac ttttctataa atatcagcag     1380

ttttcccttt cctccattcc tcttcttgtc ttttttctta ctttcccttt tttatacctt     1440

ttcattatca tcctttataa ttgtctaacc aacaactata tatctatcaa ccatgactgt     1500

tcacgacatt attgctacct actttactaa gtggtacgtc attgttccat tggccttaat     1560

cgcctataga gttttggatt acttttacgg tagatacttg atgtacaaat tgggtgctaa     1620

accattcttt cagaaacaaa ctgacggttg ttttggtttt aaggcccctt tagaattatt     1680

gaagaaaaag tctgacggta cattgatcga ttttaccttg caaagaatcc acgacttgga     1740

cagaccagat atcccaacct ttacctttcc tgttttctca atcaatttgg tgaatacttt     1800

ggaacctgaa aacatcaagg ctatcttggc tacccagttc aatgatttta gtttgggcac     1860

cagacactca cattttgcac cattgttggg agatggtatc tttacattgg atggtgctgg     1920

ttggaagcat tcaagatcca tgttaagacc acaatttgca agagagcaga tttcccatgt     1980

taagttgttg gaaccacatg ttcaagtctt cttcaagcat gttagaaaag cccaaggtaa     2040

gacttttgat attcaggagt tgttctttag attgactgtt gattctgcca ccgaattttt     2100

gtttggtgaa tccgtcgaat ccttgagaga cgaatctatt ggtatgtcta ttaacgcttt     2160

ggattttgat ggtaaggctg gatttgcaga tgcatttaac tattcccaaa actatttagc     2220

ttctagagct gtcatgcaac aattatactg ggttttaaat ggtaaaaagt tcaaggaatg     2280

taatgctaag gtccacaagt tcgctgacta ttatgtcaac aaggctttag acttaactcc     2340

agagcaatta gaaaagcagg atggttacgt cttcttatat gaattggtca aacagactag     2400

agataagcaa gttttgagag atcagttatt gaatatcatg gtcgctggta gagacacaac     2460

tgctggtttg ttgtcctttg tcttctttga attggccaga aacccagaag tcaccaacaa     2520

attaagagag gaaatcgaag ataaatttgg attaggcgaa aatgcaagtg tcgaggacat     2580

ttcctttgag tctttgaagt cttgtgaata tttgaaagct gttttgaacg agacattgcg     2640

tttgtaccct tcagttccac agaactttag agttgcaacc aagaacacca cattgcctag     2700

aggcggtggt aaagatggtt tgtctcctgt tttggtgaga aagggtcaaa ccgttattta     2760

cggtgtctac gccgcccaca gaaacccagc agtctatggt aaagatgctt tggaattcag     2820

accagagcgt tggttcgaac ctgaaaccaa aaagttgggt tgggccttct tacctttcaa     2880

cggaggtcca agaatctgtt tgggtcagca attcgccttg acagaagcct catatgtgac     2940

tgtgagattg ttgcaagaat tcgctcactt gtctatggac cctgacactg aataccctcc     3000

taaaaagatg tcccatttga ccatgtcatt atttgacggt gctaatattg aaatgtatta     3060

ggcggccgca agaaaaaaga aaaggtaaag aacttcattt gagatgaact tttgtatatg     3120

acttttagtt tctacttttt tttttattta ttgcttaatt ttctttattt caatccccca     3180

tagtttgtgt agaatatatt tattcattct ggtaactcaa acacgtagca agctcgttgc     3240

atctcgcctc gtcacgggta cagctctgga accaaagaca aaaaaaaaag ttgatccgaa     3300

ccctctcgct attccttgct atgctatcca cgagatgggg tttatcagcc caggcaagtc     3360

actaaaggat cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc     3420

agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact     3480

aaattttccc tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa     3540

agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat     3600

cacgtttctt tttcttgaaa tttttttttt tagttttttt ctctttcagt gacctccatt     3660

gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc     3720

tattacaact ttttttactt cttgttcatt agaaagaaag catagcaatc taatctaagg     3780

ggcggtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg     3840

aggaactaaa ccatgtctaa attaacctct gctgttccag tgttaaccgc ccgtgatgtt     3900

gccggtgcag tggaattttg gactgaccgt ttgggtttct cacgtgactt tgtcgaagat     3960

gattttgctg gcgttgtgcg tgatgacgtc actttgttca tctctgctgt tcaggatcag     4020

gtcgtcccag acaacacttt ggcctgggtc tgggttcgtg gtttggacga attgtacgct     4080

gagtggagtg aagttgtgtc tacaaacttt cgtgatgcat caggtccagc tatgaccgaa     4140

attggcgaac aaccttgggg ccgtgagttc gctttacgtg atccagccgg taattgcgtg     4200

cacttcgttg ctgaggagca agattagcac gtccgacggc ggcccacggg tcccaggcct     4260

cggagatccg tccccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca     4320

ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt     4380

ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca     4440

aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt     4500

gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagctggaga ccaacatgtg     4560

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     4620

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     4680

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     4740

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     4800

gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     4860

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     4920

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     4980

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     5040

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     5100

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     5160

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     5220

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     5280

atc                                                                   5283


<210>  73
<211>  1572
<212>  DNA
<213>  Candida tropicalis

<400>  73
atggcaaccc aggaaattat tgattctgtt ttaccttact tgaccaagtg gtatactgtc       60

attaccgccg ctgtgttggt gttcttgatt tctacaaaca ttaagaacta cgttaaagcc      120

aagaagttga agtgtgttga cccaccatac ttaaaagacg ctggtttgac cggtatttct      180

tcattgatcg ctgctattaa ggccaagaat gacggtcgtt tggccaactt tgccgacgag      240

gttttcgatg aataccctaa ccacaccttc tacttgtctg tggcaggagc tttgaagatt      300

gttatgaccg ttgacccaga aaatatcaaa gctgttttgg caacacagtt cacagacttt      360

tcattgggta ctcgtcatgc ccattttgct ccattgttgg gtgatggtat ttttacttta      420

gacggtgaag gttggaagca ttcaagagcc atgttgcgtc cacagtttgc cagagatcaa      480

atcggtcatg tgaaagcctt agaaccacat atccaaatta tggccaagca aatcaagttg      540

aaccaaggaa agactttcga tatccaagaa ttgttcttca gatttacagt tgacacagct      600

actgaattct tgtttggtga atctgttcac agtttatatg acgaaaagtt gggtattcca      660

accccaaatg aaatcccagg tagagaaaat ttcgctgctg cattcaatgt ctctcaacac      720

tacttggcta caagatctta cagtcaaact ttctactttt tgactaaccc aaaggaattt      780

agagactgta acgccaaggt tcaccatttg gccaagtact ttgtgaataa agctttgaat      840

ttcaccccag aggaattaga agaaaagtct aaatccggct atgttttctt atacgaatta      900

gttaaacaaa caagagatcc taaggttttg caagaccaat tgttgaacat tatggtcgct      960

ggtagagaca ctactgctgg tttattgtcc tttgctttgt ttgaattggc tagacatcca     1020

gaaatgtggt caaagttgag agaggaaatc gaagttaact ttggcgtggg tgaagattca     1080

agagttgaag agattacctt tgaggccttg aaaagatgtg aatacttgaa ggctatcttg     1140

aacgagacat tgagaatgta tccatctgtt cctgttaact ttagaacagc cactagagat     1200

acaaccttgc caagaggtgg tggtgccaac ggtacagatc caatttacat tcctaagggt     1260

tccaccgtcg cttacgtcgt gtacaaaacc caccgtttgg aggaatatta cggtaaggac     1320

gctaacgatt tccgtcctga aagatggttt gaaccaagta ctaagaagtt gggatgggcc     1380

tatgttcctt ttaacggtgg tccaagagtt tgtttgggtc agcagtttgc tttgactgaa     1440

gccagttacg ttatcaccag attagcccaa atgttcgaaa ccgtttcatc tgacccagga     1500

ttggagtatc caccaccaaa gtgtattcat ttgacaatgt cccataatga tggtgttttt     1560

gtcaagatgt ag                                                         1572


<210>  74
<211>  5286
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic integration vector for integrating CYP52A12 under control
       of the isocitrate lyase promoter

<400>  74
agatctgaat tctattaggc gaagaggcat ctagtagtag tggcagtggt gagaacgtgg       60

gcgctgctat agtgaacaat ctccagtcga tggttaagaa gaagagtgac aaaccagcag      120

tgaatgactt gtctgggtcc gtgaggaaaa gaaagaagcc cgacacaaag gacagtaacg      180

tcaagaaacc caagaaatag gggggacctg tttagatgta taggaataaa aactccgaga      240

tgatctcaat gtgtaatgga gttgtaatat tgcaaagggg gaaaatcaag actcaaacgt      300

gtgtatgagt gagcgtacgt atatctccga gagtagtatg acataatgat gactgtgaat      360

catcgtaatc tcacacaaaa accccattgt cggccatata ccacaccaag caacaccaca      420

tatcccccgg aaaaaaaaac gtgaaaaaaa gaaacaatca aaactacaac ctactccttg      480

atcacacagt cattgatcaa gttacagttc ctgctaggga atgaccaagg tacaaatcag      540

caccttaatg gttagcacgc tctcttactc tctctcacag tcttccggcc cctattcaaa      600

attctgcact tccatttgac cccagggttg ggaaacaggg ccacaaaaga aaaacccgac      660

gtgaatgaaa aaactaagaa aagaaaaaaa attatcacac cagaaattta cctaattggg      720

taattcccat cggtgttttt cctggattgt cgcacgcacg catgctgaaa aaagtgttcg      780

agttttgctt ttgcctcgga gtttcacgca agtttttcga tctcggaacc ggagggcggt      840

cgccttgttg tttgtgatgt cgtgctttgg gtgttctaat gtgctgttat tgtgctcttt      900

ttttttcttc tttttttggt gatcatatga tattgctcgg tagattactt tcgtgtgtag      960

gtattctttt agacgtttgg ttattgggta gatatgagag agagagagtg ggtgggggag     1020

gagttggttg taggagggac ccctgggagg aagtgtagtt gagttttccc tgacgaatga     1080

aaatacgttt ttgagaagat aatacaggaa aggtgtgtcg gtgaatttcc atctatccga     1140

ggatatgagt ggaggagagt cgtgtgcgtg tggttaattt aggatcagtg gaacacacaa     1200

agtaactaag acagagagac agagagaaaa atctggggaa gagacaaaga gtcagagtgt     1260

gtgagttatt ctgtattgtg aaattttttt gcccaactac ataatattgc tgaaactaat     1320

tttacttaaa aagaaaagcc aacaacgtcc ccagtaaaac ttttctataa atatcagcag     1380

ttttcccttt cctccattcc tcttcttgtc ttttttctta ctttcccttt tttatacctt     1440

ttcattatca tcctttataa ttgtctaacc aacaactata tatctatcaa ccatggcaac     1500

ccaggaaatt attgattctg ttttacctta cttgaccaag tggtatactg tcattaccgc     1560

cgctgtgttg gtgttcttga tttctacaaa cattaagaac tacgttaaag ccaagaagtt     1620

gaagtgtgtt gacccaccat acttaaaaga cgctggtttg accggtattt cttcattgat     1680

cgctgctatt aaggccaaga atgacggtcg tttggccaac tttgccgacg aggttttcga     1740

tgaataccct aaccacacct tctacttgtc tgtggcagga gctttgaaga ttgttatgac     1800

cgttgaccca gaaaatatca aagctgtttt ggcaacacag ttcacagact tttcattggg     1860

tactcgtcat gcccattttg ctccattgtt gggtgatggt atttttactt tagacggtga     1920

aggttggaag cattcaagag ccatgttgcg tccacagttt gccagagatc aaatcggtca     1980

tgtgaaagcc ttagaaccac atatccaaat tatggccaag caaatcaagt tgaaccaagg     2040

aaagactttc gatatccaag aattgttctt cagatttaca gttgacacag ctactgaatt     2100

cttgtttggt gaatctgttc acagtttata tgacgaaaag ttgggtattc caaccccaaa     2160

tgaaatccca ggtagagaaa atttcgctgc tgcattcaat gtctctcaac actacttggc     2220

tacaagatct tacagtcaaa ctttctactt tttgactaac ccaaaggaat ttagagactg     2280

taacgccaag gttcaccatt tggccaagta ctttgtgaat aaagctttga atttcacccc     2340

agaggaatta gaagaaaagt ctaaatccgg ctatgttttc ttatacgaat tagttaaaca     2400

aacaagagat cctaaggttt tgcaagacca attgttgaac attatggtcg ctggtagaga     2460

cactactgct ggtttattgt cctttgcttt gtttgaattg gctagacatc cagaaatgtg     2520

gtcaaagttg agagaggaaa tcgaagttaa ctttggcgtg ggtgaagatt caagagttga     2580

agagattacc tttgaggcct tgaaaagatg tgaatacttg aaggctatct tgaacgagac     2640

attgagaatg tatccatctg ttcctgttaa ctttagaaca gccactagag atacaacctt     2700

gccaagaggt ggtggtgcca acggtacaga tccaatttac attcctaagg gttccaccgt     2760

cgcttacgtc gtgtacaaaa cccaccgttt ggaggaatat tacggtaagg acgctaacga     2820

tttccgtcct gaaagatggt ttgaaccaag tactaagaag ttgggatggg cctatgttcc     2880

ttttaacggt ggtccaagag tttgtttggg tcagcagttt gctttgactg aagccagtta     2940

cgttatcacc agattagccc aaatgttcga aaccgtttca tctgacccag gattggagta     3000

tccaccacca aagtgtattc atttgacaat gtcccataat gatggtgttt ttgtcaagat     3060

gtaggcggcc gcaagaaaaa agaaaaggta aagaacttca tttgagatga acttttgtat     3120

atgactttta gtttctactt ttttttttat ttattgctta attttcttta tttcaatccc     3180

ccatagtttg tgtagaatat atttattcat tctggtaact caaacacgta gcaagctcgt     3240

tgcatctcgc ctcgtcacgg gtacagctct ggaaccaaag acaaaaaaaa aagttgatcc     3300

gaaccctctc gctattcctt gctatgctat ccacgagatg gggtttatca gcccaggcaa     3360

gtcactaaag gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct     3420

tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat     3480

actaaatttt ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg     3540

aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt     3600

tatcacgttt ctttttcttg aaattttttt ttttagtttt tttctctttc agtgacctcc     3660

attgatattt aagttaataa acggtcttca atttctcaag tttcagtttc atttttcttg     3720

ttctattaca acttttttta cttcttgttc attagaaaga aagcatagca atctaatcta     3780

aggggcggtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag     3840

gtgaggaact aaaccatgtc taaattaacc tctgctgttc cagtgttaac cgcccgtgat     3900

gttgccggtg cagtggaatt ttggactgac cgtttgggtt tctcacgtga ctttgtcgaa     3960

gatgattttg ctggcgttgt gcgtgatgac gtcactttgt tcatctctgc tgttcaggat     4020

caggtcgtcc cagacaacac tttggcctgg gtctgggttc gtggtttgga cgaattgtac     4080

gctgagtgga gtgaagttgt gtctacaaac tttcgtgatg catcaggtcc agctatgacc     4140

gaaattggcg aacaaccttg gggccgtgag ttcgctttac gtgatccagc cggtaattgc     4200

gtgcacttcg ttgctgagga gcaagattag cacgtccgac ggcggcccac gggtcccagg     4260

cctcggagat ccgtccccct tttcctttgt cgatatcatg taattagtta tgtcacgctt     4320

acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga     4380

agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt     4440

tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac     4500

cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaagctgg agaccaacat     4560

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     4620

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     4680

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     4740

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     4800

ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     4860

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     4920

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     4980

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5040

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5100

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     5160

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     5220

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     5280

gagatc                                                                5286


<210>  75
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloned into genomic integration and expression constructs to 
       express mCherry

<400>  75
atggtttcta agggtgaaga agacaacatg gcaatcatca aggaatttat gcgttttaag       60

gtccatatgg aaggctccgt taacggccac gagttcgaga tcgagggaga aggtgagggt      120

agaccatacg aaggtactca aaccgccaag ttgaaagtta caaagggtgg tccattgcca      180

tttgcttggg atatcttgtc cccacaattt atgtacggat caaaggcata tgtcaagcat      240

cctgccgaca tcccagatta cttgaagtta tcctttccag aaggttttaa gtgggagaga      300

gttatgaact ttgaagatgg cggagttgtt actgttactc aggactcttc cttgcaagat      360

ggtgaattta tctataaagt gaaattgaga ggtactaact ttccatccga cggtccagtc      420

atgcaaaaga agacaatggg ttgggaggct tcttccgaaa gaatgtaccc agaagacggt      480

gcattgaaag gtgaaatcaa gcaacgttta aagttgaagg acggtggtca ctacgatgcc      540

gaggtcaaga ccacttataa ggctaagaag ccagtccaat tgccaggtgc ttataacgtt      600

aacatcaagt tagatattac ttcacacaac gaagactaca caatcgttga acaatatgaa      660

agagccgaag gtagacattc taccggcggc atggacgagt tatataagta g               711


<210>  76
<211>  4425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic integration vector for integrating mCherry under control 
       of the isocitrate lyase promoter

<400>  76
agatctgaat tctattaggc gaagaggcat ctagtagtag tggcagtggt gagaacgtgg       60

gcgctgctat agtgaacaat ctccagtcga tggttaagaa gaagagtgac aaaccagcag      120

tgaatgactt gtctgggtcc gtgaggaaaa gaaagaagcc cgacacaaag gacagtaacg      180

tcaagaaacc caagaaatag gggggacctg tttagatgta taggaataaa aactccgaga      240

tgatctcaat gtgtaatgga gttgtaatat tgcaaagggg gaaaatcaag actcaaacgt      300

gtgtatgagt gagcgtacgt atatctccga gagtagtatg acataatgat gactgtgaat      360

catcgtaatc tcacacaaaa accccattgt cggccatata ccacaccaag caacaccaca      420

tatcccccgg aaaaaaaaac gtgaaaaaaa gaaacaatca aaactacaac ctactccttg      480

atcacacagt cattgatcaa gttacagttc ctgctaggga atgaccaagg tacaaatcag      540

caccttaatg gttagcacgc tctcttactc tctctcacag tcttccggcc cctattcaaa      600

attctgcact tccatttgac cccagggttg ggaaacaggg ccacaaaaga aaaacccgac      660

gtgaatgaaa aaactaagaa aagaaaaaaa attatcacac cagaaattta cctaattggg      720

taattcccat cggtgttttt cctggattgt cgcacgcacg catgctgaaa aaagtgttcg      780

agttttgctt ttgcctcgga gtttcacgca agtttttcga tctcggaacc ggagggcggt      840

cgccttgttg tttgtgatgt cgtgctttgg gtgttctaat gtgctgttat tgtgctcttt      900

ttttttcttc tttttttggt gatcatatga tattgctcgg tagattactt tcgtgtgtag      960

gtattctttt agacgtttgg ttattgggta gatatgagag agagagagtg ggtgggggag     1020

gagttggttg taggagggac ccctgggagg aagtgtagtt gagttttccc tgacgaatga     1080

aaatacgttt ttgagaagat aatacaggaa aggtgtgtcg gtgaatttcc atctatccga     1140

ggatatgagt ggaggagagt cgtgtgcgtg tggttaattt aggatcagtg gaacacacaa     1200

agtaactaag acagagagac agagagaaaa atctggggaa gagacaaaga gtcagagtgt     1260

gtgagttatt ctgtattgtg aaattttttt gcccaactac ataatattgc tgaaactaat     1320

tttacttaaa aagaaaagcc aacaacgtcc ccagtaaaac ttttctataa atatcagcag     1380

ttttcccttt cctccattcc tcttcttgtc ttttttctta ctttcccttt tttatacctt     1440

ttcattatca tcctttataa ttgtctaacc aacaactata tatctatcaa ccatggtttc     1500

taagggtgaa gaagacaaca tggcaatcat caaggaattt atgcgtttta aggtccatat     1560

ggaaggctcc gttaacggcc acgagttcga gatcgaggga gaaggtgagg gtagaccata     1620

cgaaggtact caaaccgcca agttgaaagt tacaaagggt ggtccattgc catttgcttg     1680

ggatatcttg tccccacaat ttatgtacgg atcaaaggca tatgtcaagc atcctgccga     1740

catcccagat tacttgaagt tatcctttcc agaaggtttt aagtgggaga gagttatgaa     1800

ctttgaagat ggcggagttg ttactgttac tcaggactct tccttgcaag atggtgaatt     1860

tatctataaa gtgaaattga gaggtactaa ctttccatcc gacggtccag tcatgcaaaa     1920

gaagacaatg ggttgggagg cttcttccga aagaatgtac ccagaagacg gtgcattgaa     1980

aggtgaaatc aagcaacgtt taaagttgaa ggacggtggt cactacgatg ccgaggtcaa     2040

gaccacttat aaggctaaga agccagtcca attgccaggt gcttataacg ttaacatcaa     2100

gttagatatt acttcacaca acgaagacta cacaatcgtt gaacaatatg aaagagccga     2160

aggtagacat tctaccggcg gcatggacga gttatataag taggcggccg caagaaaaaa     2220

gaaaaggtaa agaacttcat ttgagatgaa cttttgtata tgacttttag tttctacttt     2280

tttttttatt tattgcttaa ttttctttat ttcaatcccc catagtttgt gtagaatata     2340

tttattcatt ctggtaactc aaacacgtag caagctcgtt gcatctcgcc tcgtcacggg     2400

tacagctctg gaaccaaaga caaaaaaaaa agttgatccg aaccctctcg ctattccttg     2460

ctatgctatc cacgagatgg ggtttatcag cccaggcaag tcactaaagg atcccccaca     2520

caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg     2580

cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc cctctttctt     2640

cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct     2700

cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga     2760

aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta agttaataaa     2820

cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac     2880

ttcttgttca ttagaaagaa agcatagcaa tctaatctaa ggggcggtgt tgacaattaa     2940

tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgtct     3000

aaattaacct ctgctgttcc agtgttaacc gcccgtgatg ttgccggtgc agtggaattt     3060

tggactgacc gtttgggttt ctcacgtgac tttgtcgaag atgattttgc tggcgttgtg     3120

cgtgatgacg tcactttgtt catctctgct gttcaggatc aggtcgtccc agacaacact     3180

ttggcctggg tctgggttcg tggtttggac gaattgtacg ctgagtggag tgaagttgtg     3240

tctacaaact ttcgtgatgc atcaggtcca gctatgaccg aaattggcga acaaccttgg     3300

ggccgtgagt tcgctttacg tgatccagcc ggtaattgcg tgcacttcgt tgctgaggag     3360

caagattagc acgtccgacg gcggcccacg ggtcccaggc ctcggagatc cgtccccctt     3420

ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac     3480

atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt     3540

ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct     3600

gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg     3660

acgctcgaag gctttaattt gcaagctgga gaccaacatg tgagcaaaag gccagcaaaa     3720

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3780

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3840

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3900

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg     3960

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     4020

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4080

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4140

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4200

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4260

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4320

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4380

tcagtggaac gaaaactcac gttaagggat tttggtcatg agatc                     4425


<210>  77
<211>  25
<212>  DNA
<213>  Candida Tropicalis

<400>  77
tggcggaagt gcatgtgaca caacg                                             25


<210>  78
<211>  25
<212>  DNA
<213>  Candida Tropicalis

<400>  78
gtggttggtt tgtctgagtg gagag                                             25


<210>  79
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for analysis of strains

<400>  79
tggtactggt tctcgggagc acagg                                             25


<210>  80
<211>  30
<212>  DNA
<213>  Candida tropicalis

<400>  80
cgctagacaa attcttccaa aaattttaga                                        30


<210>  81
<211>  25
<212>  DNA
<213>  Candida tropicalis

<400>  81
catgtggccg ctgaatgtgg gggca                                             25


<210>  82
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer for analysis of strains

<400>  82
gccattttgt ttttttttac ccctctaaca                                        30


<210>  83
<211>  25
<212>  DNA
<213>  Candida tropicalis

<400>  83
ggaagtgcat gtgacacaat accct                                             25


<210>  84
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer for analysis of strains

<400>  84
ggtggtttgt ctgagtgaga acgtttaatt                                        30


<210>  85
<211>  25
<212>  DNA
<213>  Candida tropicalis

<400>  85
gacgtagccg atgaatgtgg ggtgc                                             25


<210>  86
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer for analysis of strains

<400>  86
tgccatttat tttttattac ccctctaaat                                        30


<210>  87
<211>  25
<212>  DNA
<213>  Candidida tropicalis

<400>  87
attggcgtcg tggcattggc ggctc                                             25


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primers for analysis of strains

<400>  88
tgggcggaat caagtggctt                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Candida tropicalis

<400>  89
cgtcgacacc cttatgttat                                                   20


<210>  90
<211>  21
<212>  DNA
<213>  Candida tropicalis

<400>  90
cgttgactcc tatcaaggac a                                                 21


<210>  91
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer used for analysis of strains

<400>  91
ggtcttctct tcctggataa tg                                                22


<210>  92
<211>  20
<212>  DNA
<213>  Candida tropicalis

<400>  92
ccagcagttg tttgttcttg                                                   20


<210>  93
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer used for analysis of strains

<400>  93
aatcctgtgc tttgtcgtag gc                                                22


<210>  94
<211>  21
<212>  DNA
<213>  Candida tropicalis

<400>  94
tccttaacaa gaagggcatc g                                                 21


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TTCTTGAATCCGGAGTTGAC

<400>  95
ttcttgaatc cggagttgac                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Candida tropicalis

<400>  96
tcttagtcgt gataccacca                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer used for analysis of strains

<400>  97
ctaaggattc tcttggcacc                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer used for analysis of strains

<400>  98
gtgaccatag gattagcacc                                                   20


<210>  99
<211>  30
<212>  DNA
<213>  Candida tropicalis

<400>  99
cttttctgat tcttgatttt cccttttcat                                        30


<210>  100
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for analysis of strains

<400>  100
atacatctag tatataagtg tcgtatttcc                                        30


<210>  101
<211>  30
<212>  DNA
<213>  Candida tropicalis

<400>  101
tgcttttctg attcttgatc atccccttag                                        30


<210>  102
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for analysis of strains

<400>  102
atacatctag tatataagtg tcgtatttct                                        30


<210>  103
<211>  25
<212>  DNA
<213>  Candida tropicalis

<400>  103
cgccagtctt tcctgattgg gcaag                                             25


<210>  104
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Analysis of constructs

<400>  104
ggacgttgtc gagtagaggg atgtg                                             25


<210>  105
<211>  20
<212>  DNA
<213>  Candida tropicalis

<400>  105
ctgtacttcc gtacttgacc                                                   20


<210>  106
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Analysis of strains

<400>  106
gagacctgga tcagatgag                                                    19


<210>  107
<211>  21
<212>  DNA
<213>  Candida albicans

<400>  107
gtttacaaag ccttaaagac t                                                 21


<210>  108
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Analysis of strains

<400>  108
ttgaacggcc aaagaaccta a                                                 21


<210>  109
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  109
gaattagaat acaaagatat cccagtg                                           27


<210>  110
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for analysis of strains

<400>  110
catcaacttg aagacctgtg gcaat                                             25


<210>  111
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  111
gaacggttcc tgtatgtcct gtgagtt                                           27


<210>  112
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  112
cggattggtc aatggctttt tcggaa                                            26


<210>  113
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  113
aaattagaat acaaggacat cccagtt                                           27


<210>  114
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  114
catcaacttg tagacttctg gcaat                                             25


<210>  115
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  115
gaacggttcc tgtatgaact gtgagta                                           27


<210>  116
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of strains

<400>  116
cagattggtt gatggccttt tcggag                                            26


<210>  117
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  117
aagttagaat acaaagacgt gccggtc                                           27


<210>  118
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for analysis of probes

<400>  118
catcaagtca aaaatctctg gcact                                             25


<210>  119
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  119
ccattgcaat acaccgatat cccagtt                                           27


<210>  120
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer for analysis of genes

<400>  120
caacaatttg aaaatctctg gcaat                                             25


<210>  121
<211>  27
<212>  DNA
<213>  Candida tropicalis

<400>  121
gaatggttcg tgtatgaact gtgagtt                                           27


<210>  122
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of strains

<400>  122
ccgactggtt gattgccttt tcggac                                            26


<210>  123
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of primers

<400>  123
cagactggtt gatggctttt tcagaa                                            26


<210>  124
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  124
ggatccgtct gaagaaatca agaacc                                            26


<210>  125
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  125
tggtgtaggc caataattgc ttaatgatat acaaaactgg caccacaa                    48


<210>  126
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  126
gagcaattgt tggaatattg gtacgttgtg gtgccagttt tgtatatca                   49


<210>  127
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  127
cgaacttaac aatagcaccg tcttgcaaac acatggtcaa gttagttaa                   49


<210>  128
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of strains

<400>  128
cgattaaggc caatggaaca atgacgtacc acttagtaaa gtaggta                     47


<210>  129
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe for analysis of strains

<400>  129
catgactgtt cacgacatta ttgctaccta ctttactaag tggtacgtc                   49


<210>  130
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of strains

<400>  130
acatttcaat attagcaccg tcaaataatg acatggtcaa atgggaca                    48


<210>  131
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Analysis of probes

<400>  131
atcaataatt tcctgggttg ccat                                              24


<210>  132
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Analysis of probes

<400>  132
atggcaaccc aggaaattat tgat                                              24


<210>  133
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CTACATCTTGACAAAAACACCATCATT

<400>  133
ctacatcttg acaaaaacac catcatt                                           27


<210>  134
<211>  709
<212>  PRT
<213>  Candida tropicalis

<400>  134

Met Thr Phe Thr Lys Lys Asn Val Ser Val Ser Gln Gly Pro Asp Pro 
1               5                   10                  15      


Arg Ser Ser Ile Gln Lys Glu Arg Asp Ser Ser Lys Trp Asn Pro Gln 
            20                  25                  30          


Gln Met Asn Tyr Phe Leu Glu Gly Ser Val Glu Arg Ser Glu Leu Met 
        35                  40                  45              


Lys Ala Leu Ala Gln Gln Met Glu Arg Asp Pro Ile Leu Phe Thr Asp 
    50                  55                  60                  


Gly Ser Tyr Tyr Asp Leu Thr Lys Asp Gln Gln Arg Glu Leu Thr Ala 
65                  70                  75                  80  


Val Lys Ile Asn Arg Ile Ala Arg Tyr Arg Glu Gln Glu Ser Ile Asp 
                85                  90                  95      


Thr Phe Asn Lys Arg Leu Ser Leu Ile Gly Ile Phe Asp Pro Gln Val 
            100                 105                 110         


Gly Thr Arg Ile Gly Val Asn Leu Gly Leu Phe Leu Ser Cys Ile Arg 
        115                 120                 125             


Gly Asn Gly Thr Thr Ser Gln Leu Asn Tyr Trp Ala Asn Glu Lys Glu 
    130                 135                 140                 


Thr Ala Asp Val Lys Gly Ile Tyr Gly Cys Phe Gly Met Thr Glu Leu 
145                 150                 155                 160 


Ala His Gly Ser Asn Val Ala Gly Leu Glu Thr Thr Ala Thr Phe Asp 
                165                 170                 175     


Lys Glu Ser Asp Glu Phe Val Ile Asn Thr Pro His Ile Gly Ala Thr 
            180                 185                 190         


Lys Trp Trp Ile Gly Gly Ala Ala His Ser Ala Thr His Cys Ser Val 
        195                 200                 205             


Tyr Ala Arg Leu Ile Val Asp Gly Gln Asp Tyr Gly Val Lys Thr Phe 
    210                 215                 220                 


Val Val Pro Leu Arg Asp Ser Asn His Asp Leu Met Pro Gly Val Thr 
225                 230                 235                 240 


Val Gly Asp Ile Gly Pro Lys Met Gly Arg Asp Gly Ile Asp Asn Gly 
                245                 250                 255     


Trp Ile Gln Phe Ser Asn Val Arg Ile Pro Arg Phe Phe Met Leu Gln 
            260                 265                 270         


Lys Phe Cys Lys Val Ser Ala Glu Gly Glu Val Thr Leu Pro Pro Leu 
        275                 280                 285             


Glu Gln Leu Ser Tyr Ser Ala Leu Leu Gly Gly Arg Val Met Met Val 
    290                 295                 300                 


Leu Asp Ser Tyr Arg Met Leu Ala Arg Met Ser Thr Ile Ala Leu Arg 
305                 310                 315                 320 


Tyr Ala Ile Gly Arg Arg Gln Phe Lys Gly Asp Asn Val Asp Pro Asn 
                325                 330                 335     


Asp Pro Asn Ala Leu Glu Thr Gln Leu Ile Asp Tyr Pro Leu His Gln 
            340                 345                 350         


Lys Arg Leu Phe Pro Tyr Phe Val Pro Pro Met Ser Ser Pro Ser Val 
        355                 360                 365             


Pro Ser Arg Leu Asn Thr Pro Ser Arg Pro Pro Trp Ser Asn Trp Thr 
    370                 375                 380                 


Ser Pro Leu Lys Arg Thr Thr Pro Arg Leu Ile Phe Lys Ser Ile Asp 
385                 390                 395                 400 


Asp Met Lys Ser Leu Phe Val Asp Ser Gly Ser Leu Lys Ser Thr Ala 
                405                 410                 415     


Thr Trp Leu Gly Ala Glu Ala Ile Asp Gln Cys Arg Gln Ala Cys Gly 
            420                 425                 430         


Gly His Gly His Ser Ser Tyr Asn Gly Phe Gly Lys Ala Tyr Asn Asp 
        435                 440                 445             


Trp Val Val Gln Cys Thr Trp Glu Gly Asp Asn Asn Val Leu Gly Met 
    450                 455                 460                 


Ser Val Gly Lys Pro Ile Val Lys Gln Val Ile Ser Ile Glu Asp Ala 
465                 470                 475                 480 


Gly Lys Thr Val Arg Gly Ser Thr Ala Phe Leu Asn Gln Leu Lys Glu 
                485                 490                 495     


Tyr Thr Gly Ser Asn Ser Ser Lys Val Val Leu Asn Thr Val Ala Asp 
            500                 505                 510         


Leu Asp Asp Ile Lys Thr Val Ile Lys Ala Ile Glu Val Ala Ile Ile 
        515                 520                 525             


Arg Leu Ser Gln Glu Ala Ala Ser Ile Val Lys Lys Glu Ser Phe Asp 
    530                 535                 540                 


Tyr Val Gly Ala Glu Leu Val Gln Leu Ser Lys Leu Lys Ala His His 
545                 550                 555                 560 


Tyr Leu Leu Thr Glu Tyr Ile Arg Arg Ile Asp Thr Phe Asp Gln Lys 
                565                 570                 575     


Glu Leu Ala Pro Tyr Leu Ile Thr Leu Gly Lys Leu Tyr Ala Ala Thr 
            580                 585                 590         


Ile Val Leu Asp Arg Phe Ala Gly Val Phe Leu Thr Phe Asn Val Ala 
        595                 600                 605             


Ser Thr Glu Ala Ile Thr Ala Leu Ala Ser Val Gln Ile Pro Lys Leu 
    610                 615                 620                 


Cys Ala Glu Val Arg Pro Asn Val Val Ala Tyr Thr Asp Ser Phe Gln 
625                 630                 635                 640 


Gln Ser Asp Met Ile Val Asn Ser Ala Ile Gly Arg Tyr Asp Gly Asp 
                645                 650                 655     


Ile Tyr Glu Asn Tyr Phe Asp Leu Val Lys Leu Gln Asn Pro Pro Ser 
            660                 665                 670         


Lys Thr Lys Ala Pro Tyr Ser Asp Ala Leu Glu Ala Met Leu Asn Arg 
        675                 680                 685             


Pro Thr Leu Asp Glu Arg Glu Arg Phe Gln Lys Ser Asp Glu Thr Ala 
    690                 695                 700                 


Ala Ile Leu Ser Lys 
705                 


<210>  135
<211>  662
<212>  PRT
<213>  Candida tropicalis

<400>  135

Met Pro Thr Glu Leu Gln Lys Glu Arg Glu Leu Thr Lys Phe Asn Pro 
1               5                   10                  15      


Lys Glu Leu Asn Tyr Phe Leu Glu Gly Ser Gln Glu Arg Ser Glu Ile 
            20                  25                  30          


Ile Ser Asn Met Val Glu Gln Met Gln Lys Asp Pro Ile Leu Lys Val 
        35                  40                  45              


Asp Ala Ser Tyr Tyr Asn Leu Thr Lys Asp Gln Gln Arg Glu Val Thr 
    50                  55                  60                  


Ala Lys Lys Ile Ala Arg Leu Ser Arg Tyr Phe Glu His Glu Tyr Pro 
65                  70                  75                  80  


Asp Gln Gln Ala Gln Arg Leu Ser Ile Leu Gly Val Phe Asp Pro Gln 
                85                  90                  95      


Val Phe Thr Arg Ile Gly Val Asn Leu Gly Leu Phe Val Ser Cys Val 
            100                 105                 110         


Arg Gly Asn Gly Thr Asn Ser Gln Phe Phe Tyr Trp Thr Ile Asn Lys 
        115                 120                 125             


Gly Ile Asp Lys Leu Arg Gly Ile Tyr Gly Cys Phe Gly Met Thr Glu 
    130                 135                 140                 


Leu Ala His Gly Ser Asn Val Gln Gly Ile Glu Thr Thr Ala Thr Phe 
145                 150                 155                 160 


Asp Glu Asp Thr Asp Glu Phe Val Ile Asn Thr Pro His Ile Gly Ala 
                165                 170                 175     


Thr Lys Trp Trp Ile Gly Gly Ala Ala His Ser Ala Thr His Cys Ser 
            180                 185                 190         


Val Tyr Ala Arg Leu Lys Val Lys Gly Lys Asp Tyr Gly Val Lys Thr 
        195                 200                 205             


Phe Val Val Pro Leu Arg Asp Ser Asn His Asp Leu Glu Pro Gly Val 
    210                 215                 220                 


Thr Val Gly Asp Ile Gly Ala Lys Met Gly Arg Asp Gly Ile Asp Asn 
225                 230                 235                 240 


Gly Trp Ile Gln Phe Ser Asn Val Arg Ile Pro Arg Phe Phe Met Leu 
                245                 250                 255     


Gln Lys Tyr Cys Lys Val Ser Arg Ser Gly Glu Val Thr Met Pro Pro 
            260                 265                 270         


Ser Glu Gln Leu Ser Tyr Ser Ala Leu Ile Gly Gly Arg Val Thr Met 
        275                 280                 285             


Met Met Asp Ser Tyr Arg Met Thr Ser Arg Phe Ile Thr Ile Ala Leu 
    290                 295                 300                 


Arg Tyr Ala Ile His Arg Arg Gln Phe Lys Lys Lys Asp Thr Asp Thr 
305                 310                 315                 320 


Ile Glu Thr Lys Leu Ile Asp Tyr Pro Leu His Gln Lys Arg Leu Phe 
                325                 330                 335     


Pro Phe Leu Ala Ala Ala Tyr Leu Phe Ser Gln Gly Ala Leu Tyr Leu 
            340                 345                 350         


Glu Gln Thr Met Asn Ala Thr Asn Asp Lys Leu Asp Glu Ala Val Ser 
        355                 360                 365             


Ala Gly Glu Lys Glu Ala Ile Asp Ala Ala Ile Val Glu Ser Lys Lys 
    370                 375                 380                 


Leu Phe Val Ala Ser Gly Cys Leu Lys Ser Thr Cys Thr Trp Leu Thr 
385                 390                 395                 400 


Ala Glu Ala Ile Asp Glu Ala Arg Gln Ala Cys Gly Gly His Gly Tyr 
                405                 410                 415     


Ser Ser Tyr Asn Gly Phe Gly Lys Ala Tyr Ser Asp Trp Val Val Gln 
            420                 425                 430         


Cys Thr Trp Glu Gly Asp Asn Asn Ile Leu Ala Met Asn Val Ala Lys 
        435                 440                 445             


Pro Met Val Arg Asp Leu Leu Lys Glu Pro Glu Gln Lys Gly Leu Val 
    450                 455                 460                 


Leu Ser Ser Val Ala Asp Leu Asp Asp Pro Ala Lys Leu Val Lys Ala 
465                 470                 475                 480 


Phe Asp His Ala Leu Ser Gly Leu Ala Arg Asp Ile Gly Ala Val Ala 
                485                 490                 495     


Glu Asp Lys Gly Phe Asp Ile Thr Gly Pro Ser Leu Val Leu Val Ser 
            500                 505                 510         


Lys Leu Asn Ala His Arg Phe Leu Ile Asp Gly Phe Phe Lys Arg Ile 
        515                 520                 525             


Thr Pro Glu Trp Ser Glu Val Leu Arg Pro Leu Gly Phe Leu Tyr Ala 
    530                 535                 540                 


Asp Trp Ile Leu Thr Asn Phe Gly Ala Thr Phe Leu Gln Tyr Gly Ile 
545                 550                 555                 560 


Ile Thr Pro Asp Val Ser Arg Lys Ile Ser Ser Glu His Phe Pro Ala 
                565                 570                 575     


Leu Cys Ala Lys Val Arg Pro Asn Val Val Gly Leu Thr Asp Gly Phe 
            580                 585                 590         


Asn Leu Thr Asp Met Met Thr Asn Ala Ala Ile Gly Arg Tyr Asp Gly 
        595                 600                 605             


Asn Val Tyr Glu His Tyr Phe Glu Thr Val Lys Ala Leu Asn Pro Pro 
    610                 615                 620                 


Glu Asn Thr Lys Ala Pro Tyr Ser Lys Ala Leu Glu Asp Met Leu Asn 
625                 630                 635                 640 


Arg Pro Asp Leu Glu Val Arg Glu Arg Gly Glu Lys Ser Glu Glu Ala 
                645                 650                 655     


Ala Glu Ile Leu Ser Ser 
            660         

<210>  136
<211>  2845
<212>  DNA
<213>  Candida tropicalis

<400>  136
gagctccaat tgtaatattt cgggagaaat atcgttgggg taaaacaaca gagagagaga       60

gggagagatg gttctggtag aattataatc tggttgttgc aaatgctact gatcgactct      120

ggcaatgtct gtagctcgct agttgtatgc aacttaggtg ttatgcatac acacggttat      180

tcggttgaat tgtggagtaa aaattgtctg agttgtgtct tagctactgg ctggcccccc      240

gcgaaagata atcaaaatta cacttgtgaa tttttgcaca cacaccgatt aacatttccc      300

ttttttgtcc accgatacac gcttgcctct tctttttttt ctctgtgctt ccccctcctg      360

tgactttttc caccattgat ataaaatcaa ctccatttcc ctaaaatctc cccagattct      420

aaaaacaact tcttctcttc tgcttttcct ttttttttgt tatatttatt taccatccct      480

tttttttgaa tagttattcc ccactaacat tgttcaaatc ttcacgacat aatgactttt      540

acaaagaaaa acgttagtgt atcacaaggt cctgacccta gatcatccat ccaaaaggaa      600

agagacagct ccaaatggaa ccctcaacaa atgaactact tcttggaagg ctccgtcgaa      660

agaagtgagt tgatgaaggc tttggcccaa caaatggaaa gagacccaat cttgttcaca      720

gacggctcct actacgactt gaccaaggac caacaaagag aattgaccgc cgtcaagatc      780

aacagaatcg ccagatacag agaacaagaa tccatcgaca ctttcaacaa gagattgtcc      840

ttgattggta tctttgaccc acaggtcggt accagaattg gtgtcaacct cggtttgttc      900

ctttcttgta tcagaggtaa cggtaccact tcccaattga actactgggc taacgaaaag      960

gaaaccgctg acgttaaagg tatctacggt tgtttcggta tgaccgaatt ggcccacggt     1020

tccaacgttg ctggtttgga aaccaccgcc acatttgaca aggaatctga cgagtttgtc     1080

atcaacaccc cacacattgg tgccaccaag tggtggattg gtggtgctgc tcactccgcc     1140

acccactgtt ctgtctacgc cagattgatt gttgacggtc aagattacgg tgtcaagact     1200

tttgttgtcc cattgagaga ctccaaccac gacctcatgc caggtgtcac tgttggtgac     1260

attggtgcca agatgggtag agatggtatc gataacggtt ggatccaatt ctccaacgtc     1320

agaatcccaa gattctttat gttgcaaaag ttctgtaagg tttctgctga aggtgaagtc     1380

accttgccac ctttggaaca attgtcttac tccgccttgt tgggtggtag agtcatgatg     1440

gttttggact cctacagaat gttggctaga atgtccacca ttgccttgag atacgccatt     1500

ggtagaagac aattcaaggg tgacaatgtc gatccaaaag atccaaacgc tttggaaacc     1560

caattgatag attacccatt gcaccaaaag agattgttcc catacttggc tgctgcctac     1620

gtcatctccg ctggtgccct caaggttgaa gacaccatcc ataacacctt ggctgaattg     1680

gacgctgccg ttgaaaagaa cgacaccaag gctatcttta agtctattga cgacatgaag     1740

tcattgtttg ttgactctgg ttccttgaag tccactgcca cttggttggg tgctgaagcc     1800

attgaccaat gtagacaagc ctgtggtggt cacggttact cgtcctacaa cggcttcggt     1860

aaagcctaca acgattgggt tgtccaatgt acttgggaag gtgacaacaa tgtcttggcc     1920

atgagtgttg gtaagccaat tgtcaagcaa gttatcagca ttgaagatgc cggcaagacc     1980

gtcagaggtt ccaccgcttt cttgaaccaa ttgaaggact acactggttc caacagctcc     2040

aaggttgttt tgaacactgt tgctgacttg gacgacatca agactgtcat caaggctatt     2100

gaagttgcca tcatcagatt gtcccaagaa gctgcttcta ttgtcaagaa ggaatctttc     2160

gactatgtcg gcgctgaatt ggttcaactc tccaagttga aggctcacca ctacttgttg     2220

actgaataca tcagaagaat tgacaccttt gaccaaaagg acttggttcc atacttgatc     2280

accctcggta agttgtacgc tgccactatt gtcttggaca gatttgccgg tgtcttcttg     2340

actttcaacg ttgcctccac cgaagccatc actgctttgg cctctgtgca aattccaaag     2400

ttgtgtgctg aagtcagacc aaacgttgtt gcttacaccg actccttcca acaatccgac     2460

atgattgtca attctgctat tggtagatac gatggtgaca tctatgagaa ctactttgac     2520

ttggtcaagt tgcagaaccc accatccaag accaaggctc cttactctga tgctttggaa     2580

gccatgttga acagaccaac cttggacgaa agagaaagat ttgaaaagtc tgatgaaacc     2640

gctgctatct tgtccaagta agaatagaag agagtgactc ttttgataag agtcgcaaat     2700

ttgatttcat aagtatatat tcattatgta aagtagtaaa tggaaaattc attaaaaaaa     2760

aagcaaattt ccgttgtatg catactccga acacaaaact agccccggaa aaacccttag     2820

ttgatagttg cgaatttagg tcgac                                           2845


<210>  137
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pre-targeting construct for transformation of POX4 into Candida 
       tropicalis

<400>  137
cgtctccaaa aggaaagaga cagctccaaa tggaaccctc aacaaatgaa ctacttcttg       60

gaaggctccg tcgaaagaag tgagttgatg aaggctttgg cccaacaaat ggaaagagac      120

ccaatcttgt tcacagacgg ctcctactac gacttgacca aggaccaaca aagagaattg      180

accgccgtca agatcaacag aatcgccaga tacagagaac aagaatccat cgacactttc      240

aacaagagat tgtccttgat tggtatcttt gacccacagg tcggtaccag aattggtgtc      300

aacgcggccg ctagatcttg cgaagctcca tctcgagact attgtcttgg acagatttgc      360

cggtgtcttc ttgactttca acgttgcctc caccgaagcc atcactgctt tggcctctgt      420

gcaaattcca aagttgtgtg ctgaagtcag accaaacgtt gttgcttaca ccgactcctt      480

ccaacaatcc gacatgattg tcaattctgc tattggtaga tacgatggtg acatctatga      540

gaactacttt gacttggtca agttgcagaa cccaccatcc aagaccaagg ctccttactc      600

tgatgctttg gaagccatgt tgaacagacc aaccgagacg                            640


<210>  138
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pre-targeting construct for targeting POX4 in Candida tropicalis

<400>  138
cgtctcgcta acgaaaagga aaccgctgac gttaaaggta tctacggttg tttcggtatg       60

accgaattgg cccacggttc caacgttgct ggtttggaaa ccaccgccac atttgacaag      120

gaatctgacg agtttgtcat caacacccca cacattggtg ccaccaagtg gtggattggt      180

ggtgctgctc actccgccac ccactgttct gtctacgcca gattgattgt tgacggtcaa      240

gattacggtg tcaagacttt tgttgtccca ttgagagact ccaaccacga cctcatgcca      300

ggtgcggccg ctagatcttg cgaagctcca tctcgagcaa gttatcagca ttgaagatgc      360

cggcaagacc gtcagaggtt ccaccgcttt cttgaaccaa ttgaaggact acactggttc      420

caacagctcc aaggttgttt tgaacactgt tgctgacttg gacgacatca agactgtcat      480

caaggctatt gaagttgcca tcatcagatt gtcccaagaa gctgcttcta ttgtcaagaa      540

ggaatctttc gactatgtcg gcgctgaatt ggttcaactc tccaagttga aggctcacca      600

ctacttgttg actgaataca tcagaagaat tgacgagacg                            640


<210>  139
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of a first targeting construct for the deletion of a 
       first allele of POX4 in Candida tropicalis

<400>  139
cgtctccaaa aggaaagaga cagctccaaa tggaaccctc aacaaatgaa ctacttcttg       60

gaaggctccg tcgaaagaag tgagttgatg aaggctttgg cccaacaaat ggaaagagac      120

ccaatcttgt tcacagacgg ctcctactac gacttgacca aggaccaaca aagagaattg      180

accgccgtca agatcaacag aatcgccaga tacagagaac aagaatccat cgacactttc      240

aacaagagat tgtccttgat tggtatcttt gacccacagg tcggtaccag aattggtgtc      300

aacgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgaga ctattgtctt     4500

ggacagattt gccggtgtct tcttgacttt caacgttgcc tccaccgaag ccatcactgc     4560

tttggcctct gtgcaaattc caaagttgtg tgctgaagtc agaccaaacg ttgttgctta     4620

caccgactcc ttccaacaat ccgacatgat tgtcaattct gctattggta gatacgatgg     4680

tgacatctat gagaactact ttgacttggt caagttgcag aacccaccat ccaagaccaa     4740

ggctccttac tctgatgctt tggaagccat gttgaacaga ccaaccgaga cg             4792


<210>  140
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of a resulting second targeting construct for the 
       deletion of the second allele of POX4 in Candida tropicalis

<400>  140
cgtctcgcta acgaaaagga aaccgctgac gttaaaggta tctacggttg tttcggtatg       60

accgaattgg cccacggttc caacgttgct ggtttggaaa ccaccgccac atttgacaag      120

gaatctgacg agtttgtcat caacacccca cacattggtg ccaccaagtg gtggattggt      180

ggtgctgctc actccgccac ccactgttct gtctacgcca gattgattgt tgacggtcaa      240

gattacggtg tcaagacttt tgttgtccca ttgagagact ccaaccacga cctcatgcca      300

ggtgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgagc aagttatcag     4500

cattgaagat gccggcaaga ccgtcagagg ttccaccgct ttcttgaacc aattgaagga     4560

ctacactggt tccaacagct ccaaggttgt tttgaacact gttgctgact tggacgacat     4620

caagactgtc atcaaggcta ttgaagttgc catcatcaga ttgtcccaag aagctgcttc     4680

tattgtcaag aaggaatctt tcgactatgt cggcgctgaa ttggttcaac tctccaagtt     4740

gaaggctcac cactacttgt tgactgaata catcagaaga attgacgaga cg             4792


<210>  141
<211>  37
<212>  DNA
<213>  Candida tropicalis

<400>  141
atgactttta caaagaaaaa cgttagtgta tcacaag                                37


<210>  142
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer for evaluating contructs

<400>  142
ttacttggac aagatagcag cggtttc                                           27


<210>  143
<211>  2624
<212>  DNA
<213>  Candida tropicalis

<400>  143
gaattcacat ggctaatttg gcctcggttc cacaacgcac tcagcattaa aaaaaaaata       60

cgcaatggca gctcggtcga cgcagcagaa gcgccgacgt accgtcgcgt tgccccgccc      120

atgcctcgcc gacccctcca ccgccatcgt ttgcccattg tttgtggtag tgcgccgtga      180

cacaaaaact tgtcctgtca catgctgaag ttacaccaac ataactacta tgggattacg      240

taatcaaaaa tttcacagtt ttaacaaaaa aaaaatcata caatcaacat tgggacatct      300

tgccctcccc cacaaaactt gcttctgcat caatcatata taaacatcat gaaataagcc      360

taaactcact tctttttttt tcatccttcc tacttcttct ttcatagtaa ctactttttt      420

tttattacca cacttattca ttcataccac gctatcatgc ctaccgaact tcaaaaagaa      480

agagaactca ccaagttcaa cccaaaggag ttgaactact tcttggaagg ttcccaagaa      540

agatccgaga tcatcagcaa catggtcgaa caaatgcaaa aagaccctat cttgaaggtc      600

gacgcttcat actacaactt gaccaaagac caacaaagag aagtcaccgc caagaagatt      660

gccagactct ccagatactt tgagcacgag tacccagacc aacaggccca gagattgtcg      720

atcctcggtg tctttgaccc acaagtcttc accagaatcg gtgtcaactt gggtttgttt      780

gtttcctgtg tccgtggtaa cggtaccaac tcccagttct tctactggac cataaataag      840

ggtatcgaca agttgagagg tatctatggt tgttttggta tgactgagtt ggcccacggt      900

tccaacgtcc aaggtattga aaccaccgcc acttttgacg aagacactga cgagtttgtc      960

atcaacaccc cacacattgg tgccaccaag tggtggatcg gtggtgctgc gcactccgcc     1020

acccactgct ccgtctacgc cagattgaag gtcaaaggaa aggactacgg tgtcaagacc     1080

tttgttgtcc cattgagaga ctccaaccac gacctcgagc caggtgtgac tgttggtgac     1140

attggtgcca agatgggtag agacggtatc gataacggtt ggatccagtt ctccaacgtc     1200

agaatcccaa gattctttat gttgcaaaag tactgtaagg tttcccgtct gggtgaagtc     1260

accatgccac catctgaaca attgtcttac tcggctttga ttggtggtag agtcaccatg     1320

atgatggact cctacagaat gaccagtaga ttcatcacca ttgccttgag atacgccatc     1380

cacagaagac aattcaagaa gaaggacacc gataccattg aaaccaagtt gattgactac     1440

ccattgcatc aaaagagatt gttcccattc ttggctgccg cttacttgtt ctcccaaggt     1500

gccttgtact tagaacaaac catgaacgca accaacgaca agttggacga agctgtcagt     1560

gctggtgaaa aggaagccat tgacgctgcc attgtcgaat ccaagaaatt gttcgtcgct     1620

tccggttgtt tgaagtccac ctgtacctgg ttgactgctg aagccattga cgaagctcgt     1680

caagcttgtg gtggtcacgg ttactcgtct tacaacggtt tcggtaaagc ctactccgac     1740

tgggttgtcc aatgtacctg ggaaggtgac aacaacatct tggccatgaa cgttgccaag     1800

ccaatggtta gagacttgtt gaaggagcca gaacaaaagg gattggttct ctccagcgtt     1860

gccgacttgg acgacccagc caagttggtt aaggctttcg accacgccct ttccggcttg     1920

gccagagaca ttggtgctgt tgctgaagac aagggtttcg acattaccgg tccaagtttg     1980

gttttggttt ccaagttgaa cgctcacaga ttcttgattg acggtttctt caagcgtatc     2040

accccagaat ggtctgaagt cttgagacct ttgggtttct tgtatgccga ctggatcttg     2100

accaactttg gtgccacctt cttgcagtac ggtatcatta ccccagatgt cagcagaaag     2160

atttcctccg agcacttccc agccttgtgt gccaaggtta gaccaaacgt tgttggtttg     2220

actgatggtt tcaacttgac tgacatgatg accaatgctg ctattggtag atatgatggt     2280

aacgtctacg aacactactt cgaaactgtc aaggctttga acccaccaga aaacaccaag     2340

gctccatact ccaaggcttt ggaagacatg ttgaaccgtc cagaccttga agtcagagaa     2400

agaggtgaaa agtccgaaga agctgctgaa atcttgtcca gttaatagag cactaggttt     2460

tgataatttg gttcttacag tttatgtatt ttgattcttc cttttttaga tacttttttt     2520

tatattttat tattccttat tgatgtaacg acagtcccac tataattaac ttaaactttg     2580

ctgtaaatca gatgacaagt gtttccctgt ttgcagggga gctc                      2624


<210>  144
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pretargeting construct for deletion of POX5 allele from Candida 
       tropicalis

<400>  144
cgtctctact tcttggaagg ttcccaagaa agatccgaga tcatcagcaa catggtcgaa       60

caaatgcaaa aagaccctat cttgaaggtc gacgcttcat actacaactt gaccaaagac      120

caacaaagag aagtcaccgc caagaagatt gccagactct ccagatactt tgagcacgag      180

tacccagacc aacaggccca gagattgtcg atcctcggtg tctttgaccc acaagtcttc      240

accagaatcg gtgtcaactt gggtttgttt gtttcctgtg tccgtggtaa cggtaccaac      300

tccgcggccg ctagatcttg cgaagctcca tctcgaggac tggatcttga ccaactttgg      360

tgccaccttc ttgcagtacg gtatcattac cccagatgtc agcagaaaga tttcctccga      420

gcacttccca gccttgtgtg ccaaggttag accaaacgtt gttggtttga ctgatggttt      480

caacttgact gacatgatga ccaatgctgc tattggtaga tatgatggta acgtctacga      540

acactacttc gaaactgtca aggctttgaa cccaccagaa aacaccaagg ctccatactc      600

caaggctttg gaagacatgt tgaaccgtcc agacgagacg                            640


<210>  145
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Second POX5 Pre-Targeting Sequence for deletion of POX5 allele 
       from Candida tropicalis

<400>  145
cgtctctttg gtatgactga gttggcccac ggttccaacg tccaaggtat tgaaaccacc       60

gccacttttg acgaagacac tgacgagttt gtcatcaaca ccccacacat tggtgccacc      120

aagtggtgga tcggtggtgc tgcgcactcc gccacccact gctccgtcta cgccagattg      180

aaggtcaaag gaaaggacta cggtgtcaag acctttgttg tcccattgag agactccaac      240

cacgacttcg agccaggtgt gactgttggt gacattggtg ccaagatggg taaagacggt      300

atcgcggccg ctagatcttg cgaagctcca tctcgagtac tccgactggg ttgtccaatg      360

tacctgggaa ggtgacaaca acatcttggc catgaacgtt gccaagccaa tggttagaga      420

cttgttgaag gagccagaac aaaagggatt ggttctctcc agcgttgccg acttggacga      480

cccagccaag ttggttaagg ctttcgacca cgccctttcc ggcttggcca gagacattgg      540

tgctgttgct gaagacaagg gtttcgacat taccggtcca agtttggttt tggtttccaa      600

gttgaacgct cacagattct tgattgacgg tttcgagacg                            640


<210>  146
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of resulting first targeting construct for deletion of a
       first allele of POX5 in Candida tropicalis

<400>  146
cgtctctact tcttggaagg ttcccaagaa agatccgaga tcatcagcaa catggtcgaa       60

caaatgcaaa aagaccctat cttgaaggtc gacgcttcat actacaactt gaccaaagac      120

caacaaagag aagtcaccgc caagaagatt gccagactct ccagatactt tgagcacgag      180

tacccagacc aacaggccca gagattgtcg atcctcggtg tctttgaccc acaagtcttc      240

accagaatcg gtgtcaactt gggtttgttt gtttcctgtg tccgtggtaa cggtaccaac      300

tccgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgagg actggatctt     4500

gaccaacttt ggtgccacct tcttgcagta cggtatcatt accccagatg tcagcagaaa     4560

gatttcctcc gagcacttcc cagccttgtg tgccaaggtt agaccaaacg ttgttggttt     4620

gactgatggt ttcaacttga ctgacatgat gaccaatgct gctattggta gatatgatgg     4680

taacgtctac gaacactact tcgaaactgt caaggctttg aacccaccag aaaacaccaa     4740

ggctccatac tccaaggctt tggaagacat gttgaaccgt ccagacgaga cg             4792


<210>  147
<211>  4792
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence of resulting second targeting construct for the deletion
       of a second allele of POX5 in Candida tropicalis

<400>  147
cgtctctttg gtatgactga gttggcccac ggttccaacg tccaaggtat tgaaaccacc       60

gccacttttg acgaagacac tgacgagttt gtcatcaaca ccccacacat tggtgccacc      120

aagtggtgga tcggtggtgc tgcgcactcc gccacccact gctccgtcta cgccagattg      180

aaggtcaaag gaaaggacta cggtgtcaag acctttgttg tcccattgag agactccaac      240

cacgacttcg agccaggtgt gactgttggt gacattggtg ccaagatggg taaagacggt      300

atcgcggccg ctctagaact agtggatctg aagttcctat tctctagaaa gtataggaac      360

ttcctgcagg accacctttg attgtaaata gtaataatta ccacccttat ctaattattt      420

atttaactta tttatttatt tattatacat atatacaaat ctaataaagt gaaaatctcc      480

cccttcacac ttcacatatg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac      540

atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaagaggtc aatgccgccg      600

agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct      660

aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg      720

tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga      780

tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca      840

tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc      900

acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga      960

acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatt     1020

ttcatatgag aaccgttatc gataactaaa gcagcaactt cttctataaa aatgggttag     1080

tatgacagtc atttaaataa ggaatttttc agttggcttg gtttcaattc aatgttcgtt     1140

tttttttttt cttgctgtgt ttgtgtttgt gttgtttata gttgtgtgca ctgagcgtcg     1200

aaaaaaaaaa ttcatagtga gccgggaaat ctgtatagcc cagataacaa cacaagtcca     1260

aactagaaac tcgtcaaaca ccaaaagcaa tgttgaatca attgccttgc acaagtacac     1320

gtaggaaaac ataaaacatt gcaattttga atattgagcc ttttgtcgta acattgattg     1380

ataggattac tcaccgaatg gttttgaaac cactgccgac agatcaatca atcaatcaaa     1440

aaacgtgaac tttgaaaaag gggaagaaca gatacattga agttagccat ttccattgat     1500

cgtcacaaca tatctgataa attactttca aaattataag ctgatgtgtg tgtattatta     1560

atgtgacagt aacatcccaa acgagaaata ttatgtcgac aacaaaaaag tttgatctga     1620

attgaaaatg aagttttccc accctaccca tttgtcatat tgaaaccaat caactgatta     1680

atcaatcaat tagaattgaa gctaaactaa aacataccac cgtccatttt gaatgattat     1740

atttttttaa tattaatatc gagataatgt ttctaagaaa gaaagaaaac caggagtgaa     1800

aattagaaaa ggaaaggaaa ggaaaaaaag aaaaatctga aaatatataa aaaaaaattg     1860

tttcgttggc aataaatctt ggtgagaaca gcgaccgaaa gcaaataaga acaaaatatg     1920

agtgtattac gttgaacaac taattaacgt gtgtgtatgg atcttttttt cttttttctc     1980

tttaaccgac tataaacaac aaacattttt gggcagtgca cacactactt aatatacaca     2040

gcataaatta cacgattaga aacaaattag cttattaaaa taacctaatc aaaccgaata     2100

ttttatggta ttatgagtaa actatataat ataaatagca cacacccaca acaacaacaa     2160

aggaaaacta aaaggttttt tctttttgaa aagatcgttt tctttattat tctctagttt     2220

tgacgctcga cattttatga tggaatgaat gggatgaatc atcaaacaag agaaaatacc     2280

cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa caacaacaag     2340

atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta tatttgtttt     2400

gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa atttcaatct     2460

ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta taaaatatac     2520

aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa gaataacaag     2580

aatacaaaac cagatttcca gatttccaga atttcactct tatatgcgtc tatttatgta     2640

ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat     2700

gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt     2760

ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac     2820

tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac     2880

ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt     2940

cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat     3000

agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt     3060

atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg     3120

ttttaggact ggttcagaat tcctcaaaaa ttcatccaaa tatacaagtg gatcgatcct     3180

accccttgcg ctaaagaagt atatgtgcct actaacgctt gtctttgtct ctgtcactaa     3240

acactggatt attactccca aatacttatt ttggactaat ttaaatgatt tcggatcaac     3300

gttcttaata tcgctgaatc ttccacaatt gatgaaagta gctaggaaga ggaattggta     3360

taaagttttt gtttttgtaa atctcgaagt atactcaaac gaatttagta ttttctcagt     3420

gatctcccag atgctttcac cctcacttag aagtgcttta agcatttttt tactgtggct     3480

atttccctta tctgcttctt ccgatgattc gaactgtaat tgcaaactac ttacaatatc     3540

agtgatatca gattgatgtt tttgtccata gtaaggaata attgtaaatt cccaagcagg     3600

aatcaatttc tttaatgagg cttccaaaat tgttgctttt tgcgtcttgt atttaaactg     3660

gagtgattta ttgacaatat cgaaactcaa cgaattgctt atgatagtat tatagctcat     3720

gaatgtggct ctcttgattg ctgttccgtt atgtgtaatc atccaacata aataggttag     3780

ttcagcagca cataatgcta ttttctcacc tgaaggtctt tcaaaccttt ccacaaactg     3840

acgaacaagc accttaggtg gtgttttaca taatatatca aattgtggca tgtcgacgat     3900

tattagttaa accactgcaa aaagttgggg aaaattttgc ccatttttat accgtgtctt     3960

cgtctatcgc ctcccccact ccccaatctt tgaattattc cgaaatattc agcgaacggg     4020

gtgtacacaa aaactaacat tctcaactgc ataatttgaa aaatggcgtg ggacaagaaa     4080

aaaaaaaaat tctcaaccat agcaatcatg gaatacggta aatttgtgtt gttcggtgac     4140

tccatcaccc agtttagttg tacccagtat ggctttcatc cagcattaca gaatgtgtat     4200

atccgaaaat tggatgttat taaccgtggt ttcagtggct acaactcaga gcacgctaga     4260

caaattcttc caaaaatttt agagtcggaa accaatatca aattgatgac aatatttttt     4320

ggaactaacg atgcatacga ctacatcaat gaaatccaga cagtcgagtt agacagatat     4380

aaagataatt taagtgtaat ggtacagatg gtactagaca aaaatatcaa accaatcatt     4440

attggatccg aagttcctat tctctagaaa gtataggaac ttcctcgagt actccgactg     4500

ggttgtccaa tgtacctggg aaggtgacaa caacatcttg gccatgaacg ttgccaagcc     4560

aatggttaga gacttgttga aggagccaga acaaaaggga ttggttctct ccagcgttgc     4620

cgacttggac gacccagcca agttggttaa ggctttcgac cacgcccttt ccggcttggc     4680

cagagacatt ggtgctgttg ctgaagacaa gggtttcgac attaccggtc caagtttggt     4740

tttggtttcc aagttgaacg ctcacagatt cttgattgac ggtttcgaga cg             4792


<210>  148
<211>  30
<212>  DNA
<213>  Candida Tropicalis

<400>  148
atgcctaccg aacttcaaaa agaaagagaa                                        30


<210>  149
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for analysis of Candida tropicalis constructs

<400>  149
ttaactggac aagatttcag cagcttcttc                                        30


