                         SEQUENCE LISTING

<110>  AB Enzymes Oy
 
<120>  A Fungal Protease and Use Thereof

<130>  A9434PC

<150>  FI 20095779

<151>  2009-07-08

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of  the 5-PCR primer PRO213 used for cloning the 
       Trichoderma reesei QM6a prb1 gene

<400>  1
tccccgcgga ctgcgcatca tggccagcct tcgtcgcctt gccct                       45


<210>  2
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of  the 3-PCR primer PRO214 used for cloning the 
       Trichoderma reesei QM6a prb1 gene

<400>  2
cgcggatcct taagcactgt tcccgttgaa gatga                                  35


<210>  3
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of  the 5-PCR primer PRO245 used for cloning the 
       Fusarium graminearum ALKO1726 protease

<400>  3
acccgcggac tgcgcatcat gaccagcttc cgccgtcttg ctctc                       45


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of  the 3-PCR primer PRO246 used for cloning the 
       Fusarium graminearum ALKO1726 protease

<400>  4
cgggatcctt aagtagaggc accgttgaag gcg                                    33


<210>  5
<211>  1371
<212>  DNA
<213>  Trichoderma reesei


<220>
<221>  misc_feature
<223>  The nucleotide sequence of the full-length Trichoderma reesei 
       QM6a protease gene prb1 (Tr prb1) encoding the Prb1 protease (ID 
       121495).

<400>  5
atggccagcc ttcgtcgcct tgccctctat ctcggagccc tgctcccggc tgttctggcc       60

gctcctgctg tcaattacaa gctgcctgaa gctgttccca acaagttcat tgtcactctc      120

aaagatggtg cctctgttga tacagactct caccttacat gggtgaaaga ccttcacagg      180

cgctcactcg gcaagcgcag cactgctggt gttgagaaga cgtacaacat cgacagctgg      240

aatgcctatg ctggcgagtt cgatgaagaa accgttaagc agatcaaggc gaatcccgac      300

gtaagtattt gccctactgt tggacgagat gccaatgttc cattgcgaaa ttctaatgag      360

cataccaggt tgcttccgta gagccagact acatcatgtg gttgtctgac attgtggaag      420

acaagcgtgc tttgaccact cagactggcg ccccctgggg actcggcact gtctcccacc      480

gcacacccgg ctcaactagc tacatctatg acacttcggc tggtagcgga acattcgcct      540

atgttgttga ctctggaatc aacattgctc accagcaatt cggcggacgt gccagcctcg      600

gctacaacgc cgctggtgga gatcatgtcg acactctcgg ccacggcacg cacgtttccg      660

gaactatcgg tggctctacc tatggtgttg ctaagcaggt aagctgcttc attatacttc      720

ttcctttgca gtgcgggcct tgagcacgcc gggctgactc tgtaacgaaa ggccagctta      780

atctccgtca aggtcttcca gggcaacagc gccagcacct cggtcatcct tgacggctat      840

aattgggccg tgaacgacat tgtctcccgc aaccgcgcca gcaagtctgc catcaacatg      900

tctctcggtg gcccggcctc ttccacctgg gctacggcga tcaatgcagc ctttaacaag      960

ggcgtcctga cgatcgtggc cgccggcaat ggtgacgctc ttggaaaccc tcagcctgtc     1020

tccagtactt ctccagccaa tgtgcccaac gccatcaccg tcgcagccct tgacattaac     1080

tggcgcaccg cttccttcac caattatggt gctggcgttg acgtcttcgc tcctggtgtc     1140

aacatcctgt cttcgtggat cggctctaac actgccacaa acacgattag cggcacctcc     1200

atggccactc ctcacgttgt cggcctcgct ctttacctgc aggctcttga gggccttagc     1260

accccgactg ctgtaaccaa ccgcatcaag gccttggcta ctactggacg cgtcaccggc     1320

agtctgaatg gcagccccaa cactctcatc ttcaacggga acagtgctta a              1371


<210>  6
<211>  409
<212>  PRT
<213>  Trichoderma reesei


<220>
<221>  MISC_FEATURE
<223>  The deduced amino acid sequence of the full-length Trichoderma 
       reesei QM6a protease Prb1 (Tr_Prb1) including amino acids from 
       Met1 to Ala409.

<400>  6

Met Ala Ser Leu Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro 
1               5                   10                  15      


Ala Val Leu Ala Ala Pro Ala Val Asn Tyr Lys Leu Pro Glu Ala Val 
            20                  25                  30          


Pro Asn Lys Phe Ile Val Thr Leu Lys Asp Gly Ala Ser Val Asp Thr 
        35                  40                  45              


Asp Ser His Leu Thr Trp Val Lys Asp Leu His Arg Arg Ser Leu Gly 
    50                  55                  60                  


Lys Arg Ser Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile Asp Ser Trp 
65                  70                  75                  80  


Asn Ala Tyr Ala Gly Glu Phe Asp Glu Glu Thr Val Lys Gln Ile Lys 
                85                  90                  95      


Ala Asn Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Trp Leu 
            100                 105                 110         


Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala 
        115                 120                 125             


Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Pro Gly Ser Thr Ser 
    130                 135                 140                 


Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val 
145                 150                 155                 160 


Asp Ser Gly Ile Asn Ile Ala His Gln Gln Phe Gly Gly Arg Ala Ser 
                165                 170                 175     


Leu Gly Tyr Asn Ala Ala Gly Gly Asp His Val Asp Thr Leu Gly His 
            180                 185                 190         


Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala 
        195                 200                 205             


Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Gln Gly Asn Ser Ala 
    210                 215                 220                 


Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile 
225                 230                 235                 240 


Val Ser Arg Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly 
                245                 250                 255     


Gly Pro Ala Ser Ser Thr Trp Ala Thr Ala Ile Asn Ala Ala Phe Asn 
            260                 265                 270         


Lys Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ala Leu Gly 
        275                 280                 285             


Asn Pro Gln Pro Val Ser Ser Thr Ser Pro Ala Asn Val Pro Asn Ala 
    290                 295                 300                 


Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr 
305                 310                 315                 320 


Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu 
                325                 330                 335     


Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr 
            340                 345                 350         


Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ala 
        355                 360                 365             


Leu Glu Gly Leu Ser Thr Pro Thr Ala Val Thr Asn Arg Ile Lys Ala 
    370                 375                 380                 


Leu Ala Thr Thr Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn 
385                 390                 395                 400 


Thr Leu Ile Phe Asn Gly Asn Ser Ala 
                405                 


<210>  7
<211>  1311
<212>  DNA
<213>  Trichoderma reesei


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       proenzyme form of Trichoderma reesei Prb1 protease.

<400>  7
gctcctgctg tcaattacaa gctgcctgaa gctgttccca acaagttcat tgtcactctc       60

aaagatggtg cctctgttga tacagactct caccttacat gggtgaaaga ccttcacagg      120

cgctcactcg gcaagcgcag cactgctggt gttgagaaga cgtacaacat cgacagctgg      180

aatgcctatg ctggcgagtt cgatgaagaa accgttaagc agatcaaggc gaatcccgac      240

gtaagtattt gccctactgt tggacgagat gccaatgttc cattgcgaaa ttctaatgag      300

cataccaggt tgcttccgta gagccagact acatcatgtg gttgtctgac attgtggaag      360

acaagcgtgc tttgaccact cagactggcg ccccctgggg actcggcact gtctcccacc      420

gcacacccgg ctcaactagc tacatctatg acacttcggc tggtagcgga acattcgcct      480

atgttgttga ctctggaatc aacattgctc accagcaatt cggcggacgt gccagcctcg      540

gctacaacgc cgctggtgga gatcatgtcg acactctcgg ccacggcacg cacgtttccg      600

gaactatcgg tggctctacc tatggtgttg ctaagcaggt aagctgcttc attatacttc      660

ttcctttgca gtgcgggcct tgagcacgcc gggctgactc tgtaacgaaa ggccagctta      720

atctccgtca aggtcttcca gggcaacagc gccagcacct cggtcatcct tgacggctat      780

aattgggccg tgaacgacat tgtctcccgc aaccgcgcca gcaagtctgc catcaacatg      840

tctctcggtg gcccggcctc ttccacctgg gctacggcga tcaatgcagc ctttaacaag      900

ggcgtcctga cgatcgtggc cgccggcaat ggtgacgctc ttggaaaccc tcagcctgtc      960

tccagtactt ctccagccaa tgtgcccaac gccatcaccg tcgcagccct tgacattaac     1020

tggcgcaccg cttccttcac caattatggt gctggcgttg acgtcttcgc tcctggtgtc     1080

aacatcctgt cttcgtggat cggctctaac actgccacaa acacgattag cggcacctcc     1140

atggccactc ctcacgttgt cggcctcgct ctttacctgc aggctcttga gggccttagc     1200

accccgactg ctgtaaccaa ccgcatcaag gccttggcta ctactggacg cgtcaccggc     1260

agtctgaatg gcagccccaa cactctcatc ttcaacggga acagtgctta a              1311


<210>  8
<211>  389
<212>  PRT
<213>  Trichoderma reesei


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the proenzyme form of Trichoderma 
       reesei Prb1 protease including amino acids Ala21 to Ala 409 of 
       the full length protease.

<400>  8

Ala Pro Ala Val Asn Tyr Lys Leu Pro Glu Ala Val Pro Asn Lys Phe 
1               5                   10                  15      


Ile Val Thr Leu Lys Asp Gly Ala Ser Val Asp Thr Asp Ser His Leu 
            20                  25                  30          


Thr Trp Val Lys Asp Leu His Arg Arg Ser Leu Gly Lys Arg Ser Thr 
        35                  40                  45              


Ala Gly Val Glu Lys Thr Tyr Asn Ile Asp Ser Trp Asn Ala Tyr Ala 
    50                  55                  60                  


Gly Glu Phe Asp Glu Glu Thr Val Lys Gln Ile Lys Ala Asn Pro Asp 
65                  70                  75                  80  


Val Ala Ser Val Glu Pro Asp Tyr Ile Met Trp Leu Ser Asp Ile Val 
                85                  90                  95      


Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala Pro Trp Gly Leu 
            100                 105                 110         


Gly Thr Val Ser His Arg Thr Pro Gly Ser Thr Ser Tyr Ile Tyr Asp 
        115                 120                 125             


Thr Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val Asp Ser Gly Ile 
    130                 135                 140                 


Asn Ile Ala His Gln Gln Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn 
145                 150                 155                 160 


Ala Ala Gly Gly Asp His Val Asp Thr Leu Gly His Gly Thr His Val 
                165                 170                 175     


Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala Lys Gln Ala Ser 
            180                 185                 190         


Leu Ile Ser Val Lys Val Phe Gln Gly Asn Ser Ala Ser Thr Ser Val 
        195                 200                 205             


Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile Val Ser Arg Asn 
    210                 215                 220                 


Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly Gly Pro Ala Ser 
225                 230                 235                 240 


Ser Thr Trp Ala Thr Ala Ile Asn Ala Ala Phe Asn Lys Gly Val Leu 
                245                 250                 255     


Thr Ile Val Ala Ala Gly Asn Gly Asp Ala Leu Gly Asn Pro Gln Pro 
            260                 265                 270         


Val Ser Ser Thr Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala 
        275                 280                 285             


Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala 
    290                 295                 300                 


Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu Ser Ser Trp Ile 
305                 310                 315                 320 


Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr 
                325                 330                 335     


Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Gly Leu 
            340                 345                 350         


Ser Thr Pro Thr Ala Val Thr Asn Arg Ile Lys Ala Leu Ala Thr Thr 
        355                 360                 365             


Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn Thr Leu Ile Phe 
    370                 375                 380                 


Asn Gly Asn Ser Ala 
385                 


<210>  9
<211>  943
<212>  DNA
<213>  Trichoderma reesei


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Trichoderma reesei Prb1 protease.

<400>  9
gctttgacca ctcagactgg cgccccctgg ggactcggca ctgtctccca ccgcacaccc       60

ggctcaacta gctacatcta tgacacttcg gctggtagcg gaacattcgc ctatgttgtt      120

gactctggaa tcaacattgc tcaccagcaa ttcggcggac gtgccagcct cggctacaac      180

gccgctggtg gagatcatgt cgacactctc ggccacggca cgcacgtttc cggaactatc      240

ggtggctcta cctatggtgt tgctaagcag gtaagctgct tcattatact tcttcctttg      300

cagtgcgggc cttgagcacg ccgggctgac tctgtaacga aaggccagct taatctccgt      360

caaggtcttc cagggcaaca gcgccagcac ctcggtcatc cttgacggct ataattgggc      420

cgtgaacgac attgtctccc gcaaccgcgc cagcaagtct gccatcaaca tgtctctcgg      480

tggcccggcc tcttccacct gggctacggc gatcaatgca gcctttaaca agggcgtcct      540

gacgatcgtg gccgccggca atggtgacgc tcttggaaac cctcagcctg tctccagtac      600

ttctccagcc aatgtgccca acgccatcac cgtcgcagcc cttgacatta actggcgcac      660

cgcttccttc accaattatg gtgctggcgt tgacgtcttc gctcctggtg tcaacatcct      720

gtcttcgtgg atcggctcta acactgccac aaacacgatt agcggcacct ccatggccac      780

tcctcacgtt gtcggcctcg ctctttacct gcaggctctt gagggcctta gcaccccgac      840

tgctgtaacc aaccgcatca aggccttggc tactactgga cgcgtcaccg gcagtctgaa      900

tggcagcccc aacactctca tcttcaacgg gaacagtgct taa                        943


<210>  10
<211>  289
<212>  PRT
<213>  Trichoderma reesei


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Trichoderma reesei 
       Prb1 protease including amino acids Ala121 to Ala409 of the full 
       length enzyme.

<400>  10

Ala Leu Thr Thr Gln Thr Gly Ala Pro Trp Gly Leu Gly Thr Val Ser 
1               5                   10                  15      


His Arg Thr Pro Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Ser Ala Gly 
            20                  25                  30          


Ser Gly Thr Phe Ala Tyr Val Val Asp Ser Gly Ile Asn Ile Ala His 
        35                  40                  45              


Gln Gln Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Asp His Val Asp Thr Leu Gly His Gly Thr His Val Ser Gly Thr Ile 
65                  70                  75                  80  


Gly Gly Ser Thr Tyr Gly Val Ala Lys Gln Ala Ser Leu Ile Ser Val 
                85                  90                  95      


Lys Val Phe Gln Gly Asn Ser Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Tyr Asn Trp Ala Val Asn Asp Ile Val Ser Arg Asn Arg Ala Ser Lys 
        115                 120                 125             


Ser Ala Ile Asn Met Ser Leu Gly Gly Pro Ala Ser Ser Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Phe Asn Lys Gly Val Leu Thr Ile Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ala Leu Gly Asn Pro Gln Pro Val Ser Ser Thr 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Leu Asp Ile 
            180                 185                 190         


Asn Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Val 
        195                 200                 205             


Phe Ala Pro Gly Val Asn Ile Leu Ser Ser Trp Ile Gly Ser Asn Thr 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val Val 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Gly Leu Ser Thr Pro Thr 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ala Leu Ala Thr Thr Gly Arg Val Thr 
            260                 265                 270         


Gly Ser Leu Asn Gly Ser Pro Asn Thr Leu Ile Phe Asn Gly Asn Ser 
        275                 280                 285             


Ala 
    


<210>  11
<211>  1292
<212>  DNA
<213>  Fusarium graminearum


<220>
<221>  misc_feature
<223>  The nucleotide sequence of the full-length Fusarium graminearum 
       ALKO1726 protease gene Fg prtS8A.

<400>  11
atgaccagct tccgccgtct tgctctcgct cttggagctc tgctccctgc agtcctcgcc       60

gctcctactg agaagcgaca ggaactcact gccgcgcctg acaagtacat catcactctc      120

aagcccgagg ctactgagaa caagatcgag gctcacttga actgggtcag cgatgtccac      180

cgccgcagcc tgaacaagcg tgacacttct ggtgttgaga agaagttcaa catcagcagc      240

tggaacgcct actctggcga gttcgacaag gctaccattg atgagatcaa gaagagcccc      300

gaggttgctt tcgtcgagcc tgactacact gtctacctcg acttcgagac cgaactcact      360

gaccgtgctc tgaccaccca gagcggcgct ccttggggtc tcgcctccat ctcccgccga      420

acctccggtg gcagcaccta cacctacgac accactgccg gctccggttc ttacggatac      480

gtcgttgaca gcggcatcaa cgtcaaccac cgagacttcg gtggccgtgc ttctctcggt      540

tacaacgctg ccggtggttc ccacgtcgac accctgggcc acggtaccca cgttgctgga      600

accattgctt cttccaccta cggtgttgcc aaggctgtaa gtaaacccca cattatatgg      660

tagcatctga actttatact tactatcttt aggccaacgt catctctgtc aaggtcttca      720

ctggcaacag tgcctctacc tccactatcc tcgctggttt caactgggct gtcaacgaca      780

tcacttccaa gggccgtgct ggtcgctctg tcatcaacat gtctctcggc ggtccctctg      840

ctcagacctg gaccactgct atcaacgctg cctacaactc tggtgtcctc tccgttgttg      900

ctgccggtaa cggtgacgat ttcggccgcc ctcttcccgt ctctggccag tctcctgcca      960

acgtccccaa cgctctgacc gttgctgcca ttgactccag ctggcgcact gcctctttca     1020

ccaactacgg tgccggtgtt gatgtcttcg cccctggtgt cggcatcctc tccacctggt     1080

acacctccaa cactgctacc aactccatca gcggtacctc catggcctgc cctcacgttg     1140

ctggtcttgc tctctacctc caggttctcg agggtctttc cacccctgct gccgtcacca     1200

accgcatcaa ggctcttgct accactggcc gtgtcactgg caccctcaac ggcagcccca     1260

acctgatcgc cttcaacggt gcctctactt aa                                   1292


<210>  12
<211>  411
<212>  PRT
<213>  Fusarium graminearum


<220>
<221>  MISC_FEATURE
<223>  The deduced amino acid sequence of the full-length Fusarium 
       graminearum ALKO1726 protease (Fg_ALKO1726) including amino acids
       from Met1 to Thr411.

<400>  12

Met Thr Ser Phe Arg Arg Leu Ala Leu Ala Leu Gly Ala Leu Leu Pro 
1               5                   10                  15      


Ala Val Leu Ala Ala Pro Thr Glu Lys Arg Gln Glu Leu Thr Ala Ala 
            20                  25                  30          


Pro Asp Lys Tyr Ile Ile Thr Leu Lys Pro Glu Ala Thr Glu Asn Lys 
        35                  40                  45              


Ile Glu Ala His Leu Asn Trp Val Ser Asp Val His Arg Arg Ser Leu 
    50                  55                  60                  


Asn Lys Arg Asp Thr Ser Gly Val Glu Lys Lys Phe Asn Ile Ser Ser 
65                  70                  75                  80  


Trp Asn Ala Tyr Ser Gly Glu Phe Asp Lys Ala Thr Ile Asp Glu Ile 
                85                  90                  95      


Lys Lys Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Thr Val Tyr 
            100                 105                 110         


Leu Asp Phe Glu Thr Glu Leu Thr Asp Arg Ala Leu Thr Thr Gln Ser 
        115                 120                 125             


Gly Ala Pro Trp Gly Leu Ala Ser Ile Ser Arg Arg Thr Ser Gly Gly 
    130                 135                 140                 


Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly Ser Gly Ser Tyr Gly Tyr 
145                 150                 155                 160 


Val Val Asp Ser Gly Ile Asn Val Asn His Arg Asp Phe Gly Gly Arg 
                165                 170                 175     


Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly Ser His Val Asp Thr Leu 
            180                 185                 190         


Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ser Ser Thr Tyr Gly 
        195                 200                 205             


Val Ala Lys Ala Ala Asn Val Ile Ser Val Lys Val Phe Thr Gly Asn 
    210                 215                 220                 


Ser Ala Ser Thr Ser Thr Ile Leu Ala Gly Phe Asn Trp Ala Val Asn 
225                 230                 235                 240 


Asp Ile Thr Ser Lys Gly Arg Ala Gly Arg Ser Val Ile Asn Met Ser 
                245                 250                 255     


Leu Gly Gly Pro Ser Ala Gln Thr Trp Thr Thr Ala Ile Asn Ala Ala 
            260                 265                 270         


Tyr Asn Ser Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Asp 
        275                 280                 285             


Phe Gly Arg Pro Leu Pro Val Ser Gly Gln Ser Pro Ala Asn Val Pro 
    290                 295                 300                 


Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Ser Trp Arg Thr Ala Ser 
305                 310                 315                 320 


Phe Thr Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Gly 
                325                 330                 335     


Ile Leu Ser Thr Trp Tyr Thr Ser Asn Thr Ala Thr Asn Ser Ile Ser 
            340                 345                 350         


Gly Thr Ser Met Ala Cys Pro His Val Ala Gly Leu Ala Leu Tyr Leu 
        355                 360                 365             


Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ala Val Thr Asn Arg Ile 
    370                 375                 380                 


Lys Ala Leu Ala Thr Thr Gly Arg Val Thr Gly Thr Leu Asn Gly Ser 
385                 390                 395                 400 


Pro Asn Leu Ile Ala Phe Asn Gly Ala Ser Thr 
                405                 410     


<210>  13
<211>  1232
<212>  DNA
<213>  Fusarium graminearum


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       proenzyme form of Fusarium graminearum ALKO1726  protease.

<400>  13
gctcctactg agaagcgaca ggaactcact gccgcgcctg acaagtacat catcactctc       60

aagcccgagg ctactgagaa caagatcgag gctcacttga actgggtcag cgatgtccac      120

cgccgcagcc tgaacaagcg tgacacttct ggtgttgaga agaagttcaa catcagcagc      180

tggaacgcct actctggcga gttcgacaag gctaccattg atgagatcaa gaagagcccc      240

gaggttgctt tcgtcgagcc tgactacact gtctacctcg acttcgagac cgaactcact      300

gaccgtgctc tgaccaccca gagcggcgct ccttggggtc tcgcctccat ctcccgccga      360

acctccggtg gcagcaccta cacctacgac accactgccg gctccggttc ttacggatac      420

gtcgttgaca gcggcatcaa cgtcaaccac cgagacttcg gtggccgtgc ttctctcggt      480

tacaacgctg ccggtggttc ccacgtcgac accctgggcc acggtaccca cgttgctgga      540

accattgctt cttccaccta cggtgttgcc aaggctgtaa gtaaacccca cattatatgg      600

tagcatctga actttatact tactatcttt aggccaacgt catctctgtc aaggtcttca      660

ctggcaacag tgcctctacc tccactatcc tcgctggttt caactgggct gtcaacgaca      720

tcacttccaa gggccgtgct ggtcgctctg tcatcaacat gtctctcggc ggtccctctg      780

ctcagacctg gaccactgct atcaacgctg cctacaactc tggtgtcctc tccgttgttg      840

ctgccggtaa cggtgacgat ttcggccgcc ctcttcccgt ctctggccag tctcctgcca      900

acgtccccaa cgctctgacc gttgctgcca ttgactccag ctggcgcact gcctctttca      960

ccaactacgg tgccggtgtt gatgtcttcg cccctggtgt cggcatcctc tccacctggt     1020

acacctccaa cactgctacc aactccatca gcggtacctc catggcctgc cctcacgttg     1080

ctggtcttgc tctctacctc caggttctcg agggtctttc cacccctgct gccgtcacca     1140

accgcatcaa ggctcttgct accactggcc gtgtcactgg caccctcaac ggcagcccca     1200

acctgatcgc cttcaacggt gcctctactt aa                                   1232


<210>  14
<211>  391
<212>  PRT
<213>  Fusarium graminearum


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the proenzyme form of Fusarium 
       graminearum ALKO1726 protease including amino acids Ala21 to 
       Thr411 of the full length protease.

<400>  14

Ala Pro Thr Glu Lys Arg Gln Glu Leu Thr Ala Ala Pro Asp Lys Tyr 
1               5                   10                  15      


Ile Ile Thr Leu Lys Pro Glu Ala Thr Glu Asn Lys Ile Glu Ala His 
            20                  25                  30          


Leu Asn Trp Val Ser Asp Val His Arg Arg Ser Leu Asn Lys Arg Asp 
        35                  40                  45              


Thr Ser Gly Val Glu Lys Lys Phe Asn Ile Ser Ser Trp Asn Ala Tyr 
    50                  55                  60                  


Ser Gly Glu Phe Asp Lys Ala Thr Ile Asp Glu Ile Lys Lys Ser Pro 
65                  70                  75                  80  


Glu Val Ala Phe Val Glu Pro Asp Tyr Thr Val Tyr Leu Asp Phe Glu 
                85                  90                  95      


Thr Glu Leu Thr Asp Arg Ala Leu Thr Thr Gln Ser Gly Ala Pro Trp 
            100                 105                 110         


Gly Leu Ala Ser Ile Ser Arg Arg Thr Ser Gly Gly Ser Thr Tyr Thr 
        115                 120                 125             


Tyr Asp Thr Thr Ala Gly Ser Gly Ser Tyr Gly Tyr Val Val Asp Ser 
    130                 135                 140                 


Gly Ile Asn Val Asn His Arg Asp Phe Gly Gly Arg Ala Ser Leu Gly 
145                 150                 155                 160 


Tyr Asn Ala Ala Gly Gly Ser His Val Asp Thr Leu Gly His Gly Thr 
                165                 170                 175     


His Val Ala Gly Thr Ile Ala Ser Ser Thr Tyr Gly Val Ala Lys Ala 
            180                 185                 190         


Ala Asn Val Ile Ser Val Lys Val Phe Thr Gly Asn Ser Ala Ser Thr 
        195                 200                 205             


Ser Thr Ile Leu Ala Gly Phe Asn Trp Ala Val Asn Asp Ile Thr Ser 
    210                 215                 220                 


Lys Gly Arg Ala Gly Arg Ser Val Ile Asn Met Ser Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Ala Gln Thr Trp Thr Thr Ala Ile Asn Ala Ala Tyr Asn Ser Gly 
                245                 250                 255     


Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Asp Phe Gly Arg Pro 
            260                 265                 270         


Leu Pro Val Ser Gly Gln Ser Pro Ala Asn Val Pro Asn Ala Leu Thr 
        275                 280                 285             


Val Ala Ala Ile Asp Ser Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr 
    290                 295                 300                 


Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Gly Ile Leu Ser Thr 
305                 310                 315                 320 


Trp Tyr Thr Ser Asn Thr Ala Thr Asn Ser Ile Ser Gly Thr Ser Met 
                325                 330                 335     


Ala Cys Pro His Val Ala Gly Leu Ala Leu Tyr Leu Gln Val Leu Glu 
            340                 345                 350         


Gly Leu Ser Thr Pro Ala Ala Val Thr Asn Arg Ile Lys Ala Leu Ala 
        355                 360                 365             


Thr Thr Gly Arg Val Thr Gly Thr Leu Asn Gly Ser Pro Asn Leu Ile 
    370                 375                 380                 


Ala Phe Asn Gly Ala Ser Thr 
385                 390     


<210>  15
<211>  926
<212>  DNA
<213>  Fusarium graminearum


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fusarium graminearum ALKO1726 protease.

<400>  15
gctctgacca cccagagcgg cgctccttgg ggtctcgcct ccatctcccg ccgaacctcc       60

ggtggcagca cctacaccta cgacaccact gccggctccg gttcttacgg atacgtcgtt      120

gacagcggca tcaacgtcaa ccaccgagac ttcggtggcc gtgcttctct cggttacaac      180

gctgccggtg gttcccacgt cgacaccctg ggccacggta cccacgttgc tggaaccatt      240

gcttcttcca cctacggtgt tgccaaggct gtaagtaaac cccacattat atggtagcat      300

ctgaacttta tacttactat ctttaggcca acgtcatctc tgtcaaggtc ttcactggca      360

acagtgcctc tacctccact atcctcgctg gtttcaactg ggctgtcaac gacatcactt      420

ccaagggccg tgctggtcgc tctgtcatca acatgtctct cggcggtccc tctgctcaga      480

cctggaccac tgctatcaac gctgcctaca actctggtgt cctctccgtt gttgctgccg      540

gtaacggtga cgatttcggc cgccctcttc ccgtctctgg ccagtctcct gccaacgtcc      600

ccaacgctct gaccgttgct gccattgact ccagctggcg cactgcctct ttcaccaact      660

acggtgccgg tgttgatgtc ttcgcccctg gtgtcggcat cctctccacc tggtacacct      720

ccaacactgc taccaactcc atcagcggta cctccatggc ctgccctcac gttgctggtc      780

ttgctctcta cctccaggtt ctcgagggtc tttccacccc tgctgccgtc accaaccgca      840

tcaaggctct tgctaccact ggccgtgtca ctggcaccct caacggcagc cccaacctga      900

tcgccttcaa cggtgcctct acttaa                                           926


<210>  16
<211>  289
<212>  PRT
<213>  Fusarium graminearum


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Fusarium 
       graminearum ALKO1726 protease including amino acids Ala123 to 
       Thr411 of the full length enzyme.

<400>  16

Ala Leu Thr Thr Gln Ser Gly Ala Pro Trp Gly Leu Ala Ser Ile Ser 
1               5                   10                  15      


Arg Arg Thr Ser Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ser Gly Ser Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Val Asn His 
        35                  40                  45              


Arg Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ser His Val Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Ser Thr Tyr Gly Val Ala Lys Ala Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Thr Gly Asn Ser Ala Ser Thr Ser Thr Ile Leu Ala Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Gly Arg Ala Gly Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ala Gln Thr Trp Thr 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Asn Ser Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Asp Phe Gly Arg Pro Leu Pro Val Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Leu Thr Val Ala Ala Ile Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Val 
        195                 200                 205             


Phe Ala Pro Gly Val Gly Ile Leu Ser Thr Trp Tyr Thr Ser Asn Thr 
    210                 215                 220                 


Ala Thr Asn Ser Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ala Leu Ala Thr Thr Gly Arg Val Thr 
            260                 265                 270         


Gly Thr Leu Asn Gly Ser Pro Asn Leu Ile Ala Phe Asn Gly Ala Ser 
        275                 280                 285             


Thr 
    


