                         SEQUENCE LISTING

<110>  Fabbrica Italiana Sintetici S.p.A.
 
<120>  Process for the preparation of testosteron

<130>  E0077796

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  323
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Asp Ser Lys Gln Gln Thr Val Arg Leu Ser Asp Gly His Phe Ile 
1               5                   10                  15      


Pro Ile Leu Gly Phe Gly Thr Tyr Ala Pro Gln Glu Val Pro Lys Ser 
            20                  25                  30          


Lys Ala Thr Glu Ala Thr Lys Ile Ala Ile Asp Ala Gly Phe Arg His 
        35                  40                  45              


Ile Asp Ser Ala Ser Met Tyr Gln Asn Glu Lys Glu Val Gly Leu Ala 
    50                  55                  60                  


Ile Arg Ser Lys Ile Ala Asp Gly Thr Val Lys Arg Glu Asp Ile Phe 
65                  70                  75                  80  


Tyr Thr Ser Lys Val Trp Cys Thr Phe His Arg Pro Glu Leu Val Arg 
                85                  90                  95      


Val Cys Leu Glu Gln Ser Leu Lys Gln Leu Gln Leu Asp Tyr Val Asp 
            100                 105                 110         


Leu Tyr Leu Ile His Phe Pro Met Ala Met Lys Pro Gly Glu Asn Tyr 
        115                 120                 125             


Leu Pro Lys Asp Glu Asn Gly Lys Leu Ile Tyr Asp Ala Val Asp Ile 
    130                 135                 140                 


Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala Gly Leu Ala 
145                 150                 155                 160 


Lys Ser Ile Gly Val Ser Asn Phe Asn Arg Arg Gln Leu Glu Lys Ile 
                165                 170                 175     


Leu Lys Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys Asn Gln Val Glu 
            180                 185                 190         


Cys His Pro Tyr Leu Asn Gln Gly Lys Leu Leu Asp Phe Cys Arg Ser 
        195                 200                 205             


Lys Asp Ile Val Leu Val Ala Tyr Ser Ala Leu Gly Ser His Arg Glu 
    210                 215                 220                 


Lys Gln Trp Val Asp Gln Ser Ser Pro Val Leu Leu Asp Asn Pro Val 
225                 230                 235                 240 


Leu Gly Ser Met Ala Lys Lys Tyr Asn Arg Thr Pro Ala Leu Ile Ala 
                245                 250                 255     


Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Val Leu Ala Lys Ser Phe 
            260                 265                 270         


Ser Glu Lys Arg Ile Lys Glu Asn Met Gln Val Phe Glu Phe Gln Leu 
        275                 280                 285             


Thr Ser Glu Asp Met Lys Val Leu Asp Asp Leu Asn Lys Asn Ile Arg 
    290                 295                 300                 


Tyr Ile Ser Gly Ser Ser Phe Lys Asp His Pro Asp Phe Pro Phe Trp 
305                 310                 315                 320 


Asp Glu Tyr 
            


<210>  2
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified 17-beta-deidrogenase

<400>  2

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Asp Ser Lys Gln Gln Thr Val Arg Leu Ser Asp 
            20                  25                  30          


Gly His Phe Ile Pro Ile Leu Gly Phe Gly Thr Tyr Ala Pro Gln Glu 
        35                  40                  45              


Val Pro Lys Ser Lys Ala Thr Glu Ala Thr Lys Ile Ala Ile Asp Ala 
    50                  55                  60                  


Gly Phe Arg His Ile Asp Ser Ala Ser Met Tyr Gln Asn Glu Lys Glu 
65                  70                  75                  80  


Val Gly Leu Ala Ile Arg Ser Lys Ile Ala Asp Gly Thr Val Lys Arg 
                85                  90                  95      


Glu Asp Ile Phe Tyr Thr Ser Lys Val Trp Cys Thr Phe His Arg Pro 
            100                 105                 110         


Glu Leu Val Arg Val Cys Leu Glu Gln Ser Leu Lys Gln Leu Gln Leu 
        115                 120                 125             


Asp Tyr Val Asp Leu Tyr Leu Ile His Phe Pro Met Ala Met Lys Pro 
    130                 135                 140                 


Gly Glu Asn Tyr Leu Pro Lys Asp Glu Asn Gly Lys Leu Ile Tyr Asp 
145                 150                 155                 160 


Ala Val Asp Ile Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp 
                165                 170                 175     


Ala Gly Leu Ala Lys Ser Ile Gly Val Ser Asn Phe Asn Arg Arg Gln 
            180                 185                 190         


Leu Glu Lys Ile Leu Lys Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys 
        195                 200                 205             


Asn Gln Val Glu Cys His Pro Tyr Leu Asn Gln Gly Lys Leu Leu Asp 
    210                 215                 220                 


Phe Cys Arg Ser Lys Asp Ile Val Leu Val Ala Tyr Ser Ala Leu Gly 
225                 230                 235                 240 


Ser His Arg Glu Lys Gln Trp Val Asp Gln Ser Ser Pro Val Leu Leu 
                245                 250                 255     


Asp Asn Pro Val Leu Gly Ser Met Ala Lys Lys Tyr Asn Arg Thr Pro 
            260                 265                 270         


Ala Leu Ile Ala Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Val Leu 
        275                 280                 285             


Ala Lys Ser Phe Ser Glu Lys Arg Ile Lys Glu Asn Met Gln Val Phe 
    290                 295                 300                 


Glu Phe Gln Leu Thr Ser Glu Asp Met Lys Val Leu Asp Asp Leu Asn 
305                 310                 315                 320 


Lys Asn Ile Arg Tyr Ile Ser Gly Ser Ser Phe Lys Asp His Pro Asp 
                325                 330                 335     


Phe Pro Phe Trp Asp Glu Tyr 
            340             


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer

<400>  3
gctgagaaca tatggattct aag                                               23


<210>  4
<211>  1269
<212>  DNA
<213>  Rattus norvegicus

<400>  4
gtatcttctc agttggtggg ctgagaagcc atggattcta agcagcagac agtgcgtcta       60

agtgatggtc acttcatccc tatactgggg tttggtacct atgcacctca agaggtacct      120

aagagtaagg ctacagaagc tactaaaata gccatagatg ctggtttccg ccatattgat      180

tcgcttctat gtatcaaaat gaaaaggaag taggactagc catccgaagc aagatagcag      240

atggcactgt gaagagggaa gatatatttt acacatcaaa ggtttggtgt acttttcatc      300

gtccagaact cgtacgggtc tgcttggaac agtcattgaa gcaactccag ttggactatg      360

tggacctgta cctcattcat ttcccaatgg ccatgaagcc gggagaaaat tatctcccaa      420

aagatgaaaa tggaaaatta atatatgatg ctgtggatat ctgtgacacc tgggaagcca      480

tggagaaatg caaggatgca ggattggcca agtccattgg ggtgtccaac tttaaccgca      540

ggcagctgga gaagatcctg aaaaagccgg ggctcaagta caagcctgtg tgcaaccagg      600

tagaatgtca tccttatctc aatcagggaa aacttctgga tttctgcagg tcaaaagaca      660

ttgttctggt tgcttacagt gctctgggaa gccatcgtga aaaacaatgg gttgatcaga      720

gctctcctgt tcttttggat aatccagttc ttggctcaat ggcaaaaaag tacaatcgaa      780

ctcctgcgct gattgccctt cgctaccagc tacaacgtgg ggttgtggtc ctcgccaaga      840

gtttctctga gaagaggata aaagagaata tgcaggtttt tgaatttcag ttgacttcag      900

aggacatgaa agtcctcgat gacctgaata aaaatatccg atacataagt ggttctagct      960

ttaaggacca tcctgatttt ccattttggg atgaatacta actggaggtc cattttgtgc     1020

cttgtgccag atgtcactgc attggaagag tgtataggaa gagtattctc aaaatgtgat     1080

gattacatat caccctaatc caagcttctg agcaattctg gctctgctga atctaccatt     1140

ttaaccaaga aagccaaaac tatgtatatt tctcctttct aagaaataaa agaatcgtta     1200

ttctttagca tttaaaaaaa aaaaaaaaag ggcggccgct ctagagtatc cctcgagggg     1260

cccaagctt                                                             1269


