                         SEQUENCE LISTING

<110>  DANISCO A/S
 
<120>  PROTEIN

<130>  P037139WO

<150>  US 61/220288
<151>  2009-06-25

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA fragment encoding a lipolytic enzyme

<400>  1
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaagatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacggcg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctgctggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga cgttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  2
<211>  348
<212>  PRT
<213>  Fusarium heterosporum

<400>  2

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Gly Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Arg Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  3
<211>  8616
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression vector pTrex3

<400>  3
aagcttaact agtacttctc gagctctgta catgtccggt cgcgacgtac gcgtatcgat       60

ggcgccagct gcaggcggcc gcctgcagcc acttgcagtc ccgtggaatt ctcacggtga      120

atgtaggcct tttgtagggt aggaattgtc actcaagcac ccccaacctc cattacgcct      180

cccccataga gttcccaatc agtgagtcat ggcactgttc tcaaatagat tggggagaag      240

ttgacttccg cccagagctg aaggtcgcac aaccgcatga tatagggtcg gcaacggcaa      300

aaaagcacgt ggctcaccga aaagcaagat gtttgcgatc taacatccag gaacctggat      360

acatccatca tcacgcacga ccactttgat ctgctggtaa actcgtattc gccctaaacc      420

gaagtgcgtg gtaaatctac acgtgggccc ctttcggtat actgcgtgtg tcttctctag      480

gtgccattct tttcccttcc tctagtgttg aattgtttgt gttggagtcc gagctgtaac      540

tacctctgaa tctctggaga atggtggact aacgactacc gtgcacctgc atcatgtata      600

taatagtgat cctgagaagg ggggtttgga gcaatgtggg actttgatgg tcatcaaaca      660

aagaacgaag acgcctcttt tgcaaagttt tgtttcggct acggtgaaga actggatact      720

tgttgtgtct tctgtgtatt tttgtggcaa caagaggcca gagacaatct attcaaacac      780

caagcttgct cttttgagct acaagaacct gtggggtata tatctagagt tgtgaagtcg      840

gtaatcccgc tgtatagtaa tacgagtcgc atctaaatac tccgaagctg ctgcgaaccc      900

ggagaatcga gatgtgctgg aaagcttcta gcgagcggct aaattagcat gaaaggctat      960

gagaaattct ggagacggct tgttgaatca tggcgttcca ttcttcgaca agcaaagcgt     1020

tccgtcgcag tagcaggcac tcattcccga aaaaactcgg agattcctaa gtagcgatgg     1080

aaccggaata atataatagg caatacattg agttgcctcg acggttgcaa tgcaggggta     1140

ctgagcttgg acataactgt tccgtacccc acctcttctc aacctttggc gtttccctga     1200

ttcagcgtac ccgtacaagt cgtaatcact attaacccag actgaccgga cgtgttttgc     1260

ccttcatttg gagaaataat gtcattgcga tgtgtaattt gcctgcttga ccgactgggg     1320

ctgttcgaag cccgaatgta ggattgttat ccgaactctg ctcgtagagg catgttgtga     1380

atctgtgtcg ggcaggacac gcctcgaagg ttcacggcaa gggaaaccac cgatagcagt     1440

gtctagtagc aacctgtaaa gccgcaatgc agcatcactg gaaaatacaa accaatggct     1500

aaaagtacat aagttaatgc ctaaagaagt catataccag cggctaataa ttgtacaatc     1560

aagtggctaa acgtaccgta atttgccaac ggcttgtggg gttgcagaag caacggcaaa     1620

gccccacttc cccacgtttg tttcttcact cagtccaatc tcagctggtg atcccccaat     1680

tgggtcgctt gtttgttccg gtgaagtgaa agaagacaga ggtaagaatg tctgactcgg     1740

agcgttttgc atacaaccaa gggcagtgat ggaagacagt gaaatgttga cattcaagga     1800

gtatttagcc agggatgctt gagtgtatcg tgtaaggagg tttgtctgcc gatacgacga     1860

atactgtata gtcacttctg atgaagtggt ccatattgaa atgtaagtcg gcactgaaca     1920

ggcaaaagat tgagttgaaa ctgcctaaga tctcgggccc tcgggccttc ggcctttggg     1980

tgtacatgtt tgtgctccgg gcaaatgcaa agtgtggtag gatcgaacac actgctgcct     2040

ttaccaagca gctgagggta tgtgataggc aaatgttcag gggccactgc atggtttcga     2100

atagaaagag aagcttagcc aagaacaata gccgataaag atagcctcat taaacggaat     2160

gagctagtag gcaaagtcag cgaatgtgta tatataaagg ttcgaggtcc gtgcctccct     2220

catgctctcc ccatctactc atcaactcag atcctccagg agacttgtac accatctttt     2280

gaggcacaga aacccaatag tcaaccgcgg actgcgcatc atgtatcgga agttggccgt     2340

catctcggcc ttcttggcca cacctcgtgc tagactaggc gcgccgcgcg ccagctccgt     2400

gcgaaagcct gacgcaccgg tagattcttg gtgagcccgt atcatgacgg cggcgggagc     2460

tacatggccc cgggtgattt attttttttg tatctacttc tgaccctttt caaatatacg     2520

gtcaactcat ctttcactgg agatgcggcc tgcttggtat tgcgatgttg tcagcttggc     2580

aaattgtggc tttcgaaaac acaaaacgat tccttagtag ccatgcattt taagataacg     2640

gaatagaaga aagaggaaat taaaaaaaaa aaaaaaacaa acatcccgtt cataacccgt     2700

agaatcgccg ctcttcgtgt atcccagtac cagtttattt tgaatagctc gcccgctgga     2760

gagcatcctg aatgcaagta acaaccgtag aggctgacac ggcaggtgtt gctagggagc     2820

gtcgtgttct acaaggccag acgtcttcgc ggttgatata tatgtatgtt tgactgcagg     2880

ctgctcagcg acgacagtca agttcgccct cgctgcttgt gcaataatcg cagtggggaa     2940

gccacaccgt gactcccatc tttcagtaaa gctctgttgg tgtttatcag caatacacgt     3000

aatttaaact cgttagcatg gggctgatag cttaattacc gtttaccagt gccatggttc     3060

tgcagctttc cttggcccgt aaaattcggc gaagccagcc aatcaccagc taggcaccag     3120

ctaaacccta taattagtct cttatcaaca ccatccgctc ccccgggatc aatgaggaga     3180

atgaggggga tgcggggcta aagaagccta cataaccctc atgccaactc ccagtttaca     3240

ctcgtcgagc caacatcctg actataagct aacacagaat gcctcaatcc tgggaagaac     3300

tggccgctga taagcgcgcc cgcctcgcaa aaaccatccc tgatgaatgg aaagtccaga     3360

cgctgcctgc ggaagacagc gttattgatt tcccaaagaa atcggggatc ctttcagagg     3420

ccgaactgaa gatcacagag gcctccgctg cagatcttgt gtccaagctg gcggccggag     3480

agttgacctc ggtggaagtt acgctagcat tctgtaaacg ggcagcaatc gcccagcagt     3540

tagtagggtc ccctctacct ctcagggaga tgtaacaacg ccaccttatg ggactatcaa     3600

gctgacgctg gcttctgtgc agacaaactg cgcccacgag ttcttccctg acgccgctct     3660

cgcgcaggca agggaactcg atgaatacta cgcaaagcac aagagacccg ttggtccact     3720

ccatggcctc cccatctctc tcaaagacca gcttcgagtc aaggtacacc gttgccccta     3780

agtcgttaga tgtccctttt tgtcagctaa catatgccac cagggctacg aaacatcaat     3840

gggctacatc tcatggctaa acaagtacga cgaaggggac tcggttctga caaccatgct     3900

ccgcaaagcc ggtgccgtct tctacgtcaa gacctctgtc ccgcagaccc tgatggtctg     3960

cgagacagtc aacaacatca tcgggcgcac cgtcaaccca cgcaacaaga actggtcgtg     4020

cggcggcagt tctggtggtg agggtgcgat cgttgggatt cgtggtggcg tcatcggtgt     4080

aggaacggat atcggtggct cgattcgagt gccggccgcg ttcaacttcc tgtacggtct     4140

aaggccgagt catgggcggc tgccgtatgc aaagatggcg aacagcatgg agggtcagga     4200

gacggtgcac agcgttgtcg ggccgattac gcactctgtt gagggtgagt ccttcgcctc     4260

ttccttcttt tcctgctcta taccaggcct ccactgtcct cctttcttgc tttttatact     4320

atatacgaga ccggcagtca ctgatgaagt atgttagacc tccgcctctt caccaaatcc     4380

gtcctcggtc aggagccatg gaaatacgac tccaaggtca tccccatgcc ctggcgccag     4440

tccgagtcgg acattattgc ctccaagatc aagaacggcg ggctcaatat cggctactac     4500

aacttcgacg gcaatgtcct tccacaccct cctatcctgc gcggcgtgga aaccaccgtc     4560

gccgcactcg ccaaagccgg tcacaccgtg accccgtgga cgccatacaa gcacgatttc     4620

ggccacgatc tcatctccca tatctacgcg gctgacggca gcgccgacgt aatgcgcgat     4680

atcagtgcat ccggcgagcc ggcgattcca aatatcaaag acctactgaa cccgaacatc     4740

aaagctgtta acatgaacga gctctgggac acgcatctcc agaagtggaa ttaccagatg     4800

gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg ggaaggaact ggacgccatc     4860

atcgcgccga ttacgcctac cgctgcggta cggcatgacc agttccggta ctatgggtat     4920

gcctctgtga tcaacctgct ggatttcacg agcgtggttg ttccggttac ctttgcggat     4980

aagaacatcg ataagaagaa tgagagtttc aaggcggtta gtgagcttga tgccctcgtg     5040

caggaagagt atgatccgga ggcgtaccat ggggcaccgg ttgcagtgca ggttatcgga     5100

cggagactca gtgaagagag gacgttggcg attgcagagg aagtggggaa gttgctggga     5160

aatgtggtga ctccatagct aataagtgtc agatagcaat ttgcacaaga aatcaatacc     5220

agcaactgta aataagcgct gaagtgacca tgccatgcta cgaaagagca gaaaaaaacc     5280

tgccgtagaa ccgaagagat atgacacgct tccatctctc aaaggaagaa tcccttcagg     5340

gttgcgtttc cagtctagac acgtataacg gcacaagtgt ctctcaccaa atgggttata     5400

tctcaaatgt gatctaagga tggaaagccc agaatatcga tcgcgcgcag atccatatat     5460

agggcccggg ttataattac ctcaggtcga cgtcccatgg ccattcgaat tcgtaatcat     5520

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag     5580

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg     5640

cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa     5700

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca     5760

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     5820

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     5880

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     5940

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     6000

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     6060

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     6120

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     6180

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     6240

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     6300

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     6360

gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     6420

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     6480

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     6540

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     6600

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     6660

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     6720

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     6780

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     6840

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     6900

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     6960

cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct     7020

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     7080

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     7140

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     7200

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     7260

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac     7320

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     7380

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     7440

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     7500

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     7560

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     7620

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct     7680

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     7740

gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg     7800

tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg     7860

gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag     7920

tgcaccataa aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa     7980

tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat     8040

agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg     8100

tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac     8160

catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta     8220

aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag     8280

ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg     8340

taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg     8400

acgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca     8460

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     8520

acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     8580

ttcccagtca cgacgttgta aaacgacggc cagtgc                               8616


<210>  4
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 3"

<400>  4
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacggcg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctgctggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  5
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 3"

<400>  5

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Gly Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  6
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 4"

<400>  6
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaagatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctgctggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  7
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 4"

<400>  7

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  8
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 5"

<400>  8
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaagatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacggcg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  9
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 5"

<400>  9

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Gly Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  10
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 345"

<400>  10
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  11
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 345"

<400>  11

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  12
<211>  1065
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 3459"

<400>  12
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgctgagag catcgacatg      960

agggctacca tgacagacgc acagttggag gccaagctca actcttatgt tgccatggat     1020

caggagtatg tcaagactca ccaaaaccgc acatgaggcg cgccg                     1065


<210>  13
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 3459"

<400>  13

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Glu Ser Ile Asp Met Arg Ala Thr Met Thr 
305                 310                 315                 320 


Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met Asp Gln 
                325                 330                 335     


Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345     


<210>  14
<211>  1064
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 9"

<400>  14
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaagatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacggcg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctgctggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgctgagag catcgacatg      960

agggctacca tgacagacgc acagttggag gccaagctca actcttatgt tgccatggat     1020

caggagtatg tcaagactca ccaaaaccgc acatgaggcg cgcc                      1064


<210>  15
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 9"

<400>  15

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Gly Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Arg Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  16
<211>  1031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 10"

<400>  16
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggcgc tccaccatga cagacgcaca gttggaggcc      960

aagctcaact cttatgttgc catggatcag gagtatgtca agactcacca aaaccgcaca     1020

tgaggcgcgc c                                                          1031


<210>  17
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut10"

<400>  17

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Thr Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr 
305                 310                 315                 320 


Val Ala Met Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
                325                 330                 335 


<210>  18
<211>  1031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 11"

<400>  18
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggcgc tccgagatga cagacgcaca gttggaggcc      960

aagctcaact cttatgttgc catggatcag gagtatgtca agactcacca aaaccgcaca     1020

tgaggcgcgc c                                                          1031


<210>  19
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 11"

<400>  19

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Glu Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr 
305                 310                 315                 320 


Val Ala Met Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
                325                 330                 335 


<210>  20
<211>  1031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 12"

<400>  20
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt tggagtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaacatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacaacg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctaacggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaac tccaccatga cagacgcaca gttggaggcc      960

aagctcaact cttatgttgc catggatcag gagtatgtca agactcacca aaaccgcaca     1020

tgaggcgcgc c                                                          1031


<210>  21
<211>  334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 12"

<400>  21

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Gly Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Asn Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Asn Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Asn Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Asn Ser Thr Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr 
305                 310                 315                 320 


Val Ala Met Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg 
                325                 330                 


<210>  22
<211>  346
<212>  PRT
<213>  Fusarium oxysporum

<400>  22

Met Leu Leu Leu Pro Leu Leu Ser Ala Ile Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Asp Asp Tyr Val Asn Ser Leu Glu Glu Arg Ala Val 
            20                  25                  30          


Gly Val Thr Thr Thr Asp Phe Ser Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Glu Ala Ala Ala Gly Ser Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Val Gln Gly Asn Gly Ala Thr 
65                  70                  75                  80  


Ile Val Thr Ser Phe Val Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ala Thr Asp Ser Ala Arg Lys Glu Ile Val Val Ser Phe Arg Gly Ser 
            100                 105                 110         


Ile Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Gly Gln Glu Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Arg Ala 
    130                 135                 140                 


Trp Asn Glu Ile Ser Ser Gln Ala Thr Ala Ala Val Ala Ser Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Asn Val Ile Ser Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Val Leu Ala Ala Ala Asn Leu Arg Val Gly Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ser Pro Arg Val Gly Asn Ala Gln 
        195                 200                 205             


Leu Ser Ala Phe Val Ser Asn Gln Ala Gly Gly Glu Tyr Arg Val Thr 
    210                 215                 220                 


His Ala Asp Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Phe Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Thr Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Gly Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Ala Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Arg Tyr Arg Ser Ala Glu Ser Val Asp Lys Arg Ala Thr Met Thr 
305                 310                 315                 320 


Asp Ala Glu Leu Glu Lys Lys Leu Asn Ser Tyr Val Gln Met Asp Lys 
                325                 330                 335     


Glu Tyr Val Lys Asn Asn Gln Ala Arg Ser 
            340                 345     


<210>  23
<211>  282
<212>  PRT
<213>  Fusarium oxysporum

<400>  23

Ala Val Gly Val Thr Thr Thr Asp Phe Ser Asn Phe Lys Phe Tyr Ile 
1               5                   10                  15      


His Gly Ala Ala Ala Tyr Cys Asn Ser Glu Ala Ala Ala Gly Ser Lys 
            20                  25                  30          


Ile Thr Cys Ser Asn Asn Gly Cys Pro Thr Val Gln Gly Asn Gly Ala 
        35                  40                  45              


Thr Ile Val Thr Ser Phe Val Gly Ser Lys Thr Gly Ile Gly Gly Tyr 
    50                  55                  60                  


Val Ala Thr Asp Ser Ala Arg Lys Glu Ile Val Val Ser Phe Arg Gly 
65                  70                  75                  80  


Ser Ile Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Gly Gln Glu 
                85                  90                  95      


Asp Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Arg 
            100                 105                 110         


Ala Trp Asn Glu Ile Ser Ser Ala Thr Ala Ala Val Ala Ser Ala Arg 
        115                 120                 125             


Lys Ala Asn Pro Ser Phe Asn Val Ile Ser Thr Gly His Ser Leu Gly 
    130                 135                 140                 


Gly Ala Val Ala Val Leu Ala Ala Ala Asn Leu Arg Val Gly Gly Thr 
145                 150                 155                 160 


Pro Val Asp Ile Tyr Thr Tyr Gly Ser Pro Arg Val Gly Asn Ala Leu 
                165                 170                 175     


Ser Ala Phe Val Ser Asn Gln Ala Gly Gly Glu Tyr Arg Val Thr His 
            180                 185                 190         


Ala Asp Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr Arg 
        195                 200                 205             


His Thr Thr Pro Glu Phe Trp Leu Ser Gly Gly Gly Gly Asp Lys Val 
    210                 215                 220                 


Asp Tyr Thr Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn Leu 
225                 230                 235                 240 


Gly Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Ala Ala His Leu His 
                245                 250                 255     


Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp Arg 
            260                 265                 270         


Arg Tyr Arg Ser Ala Ser Val Asp Lys Arg 
        275                 280         


<210>  24
<211>  1170
<212>  DNA
<213>  Fusarium oxysporum

<400>  24
ttggagaata ttccttgtca cgatgcttct tctaccactc ctctcggcca tcaccctcgc       60

ggtagccagt cctgtagctc tcgacgacta cgtcaactct cttgaggagc gagctgttgg      120

tgtcactaca accgacttca gcaacttcaa gttctacatc caacacggcg ccgcagctta      180

ctgcaactct gaagccgcag ctggttccaa gatcacctgc tccaacaatg gctgtccaac      240

cgttcagggc aacggagcga ccatcgtgac atctttcgtt ggctccaaga caggtatcgg      300

tggctacgtc gcgacagact ctgcccgaaa ggaaatcgtc gtctcgttcc gcggaagcat      360

caatattcga aactggctta ccaacctcga cttcggccag gaagactgca gtctcgtctc      420

tggatgcggt gtgcactctg gcttccagcg agcctggaat gagatctcgt ctcaagcaac      480

cgctgctgtt gcctccgccc gcaaggcgaa cccttctttc aacgtcattt ctacaggcca      540

ctcccttgga ggtgccgtgg ccgttcttgc tgccgcaaac ttgagagtcg gtggaacacc      600

cgtcgatatt tacacctacg gctctccccg tgtcggaaac gcgcagctct cagccttcgt      660

ctcaaaccag gctggtggag agtaccgcgt tacacacgct gatgaccctg tcccccgtct      720

ccctcctctg atcttcggat acaggcacac aactcctgag ttctggctgt ccggcggtgg      780

aggcgacaag gttgactaca ccatcagcga tgtcaaggtc tgtgagggtg ctgccaacct      840

tggatgcaac ggtggaactc ttggtttgga tattgctgct catctgcatt acttccaggc      900

gactgacgcc tgtaacgctg gtggcttctc ttggcgacga tacagaagcg ccgagagcgt      960

cgacaagagg gccaccatga ctgatgccga gcttgagaag aagctgaact cttatgtcca     1020

gatggataag gagtatgtga agaataacca ggcccgctct taacgagggt atgaggtttg     1080

atgggaaatg acatgattca tgaacgaaac catagtacat atgatgcaaa taggatataa     1140

aaacatattt cattcactag ctttacacaa                                      1170


<210>  25
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 1"

<400>  25

Met Leu Leu Leu Ser Leu Leu Ser Ala Val Thr Leu Ala Val Ala Ser 
1               5                   10                  15      


Pro Val Ala Leu Glu Glu Tyr Ala Asn Ser Leu Glu Asp Arg Ala Val 
            20                  25                  30          


Asn Val Thr Ser Thr Asp Phe Thr Asn Phe Lys Phe Tyr Ile Gln His 
        35                  40                  45              


Gly Ala Ala Ala Tyr Cys Asn Ser Gly Thr Ala Ala Gly Ala Lys Ile 
    50                  55                  60                  


Thr Cys Ser Asn Asn Gly Cys Pro Thr Ile Glu Ser Asn Gly Val Thr 
65                  70                  75                  80  


Val Val Ala Ser Phe Thr Gly Ser Lys Thr Gly Ile Gly Gly Tyr Val 
                85                  90                  95      


Ser Thr Asp Ser Ser Arg Lys Glu Ile Val Val Ala Ile Arg Gly Ser 
            100                 105                 110         


Ser Asn Ile Arg Asn Trp Leu Thr Asn Leu Asp Phe Asp Gln Ser Asp 
        115                 120                 125             


Cys Ser Leu Val Ser Gly Cys Gly Val His Ser Gly Phe Gln Asn Ala 
    130                 135                 140                 


Trp Ala Glu Ile Ser Ala Gln Ala Ser Ala Ala Val Ala Lys Ala Arg 
145                 150                 155                 160 


Lys Ala Asn Pro Ser Phe Lys Val Val Ala Thr Gly His Ser Leu Gly 
                165                 170                 175     


Gly Ala Val Ala Thr Leu Ser Ala Ala Asn Leu Arg Ala Ala Gly Thr 
            180                 185                 190         


Pro Val Asp Ile Tyr Thr Tyr Gly Ala Pro Arg Val Gly Asn Ala Ala 
        195                 200                 205             


Leu Ser Ala Phe Ile Ser Asn Gln Ala Gly Gly Glu Phe Arg Val Thr 
    210                 215                 220                 


His Asp Lys Asp Pro Val Pro Arg Leu Pro Pro Leu Ile Phe Gly Tyr 
225                 230                 235                 240 


Arg His Thr Thr Pro Glu Tyr Trp Leu Ser Gly Gly Gly Gly Asp Lys 
                245                 250                 255     


Val Asp Tyr Ala Ile Ser Asp Val Lys Val Cys Glu Gly Ala Ala Asn 
            260                 265                 270         


Leu Met Cys Asn Gly Gly Thr Leu Gly Leu Asp Ile Asp Ala His Leu 
        275                 280                 285             


His Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly Phe Ser Trp 
    290                 295                 300                 


Arg Ser Tyr Arg Ser Ala Lys Arg Glu Ser Ile Asp Met Arg Ala Thr 
305                 310                 315                 320 


Met Thr Asp Ala Gln Leu Glu Ala Lys Leu Asn Ser Tyr Val Ala Met 
                325                 330                 335     


Asp Gln Glu Tyr Val Lys Thr His Gln Asn Arg Thr 
            340                 345             


<210>  26
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant lipolytic enzyme "mut 1"

<400>  26
ccgcggactg gcatcatgct tcttctatcc ctcctctcgg ctgtcaccct tgcggtggcc       60

agtcctgtag ccctcgaaga atacgccaac tctcttgaag acagagccgt taacgtcacc      120

tcaacagact tcaccaactt caagttctac atccagcatg gcgccgcagc atactgcaac      180

tccgggaccg cagccggtgc aaagatcacc tgttccaaca atggttgccc aacgattgag      240

tccaacggcg tgactgtcgt ggcatctttc actggctcca agactggcat cggcgggtac      300

gtctcgacag atagctcccg taaagaaatc gtcgtcgcga tccgtggtag cagcaacatc      360

cgcaactggc ttacaaacct cgactttgac cagtccgact gcagtcttgt ctctggctgt      420

ggtgtgcact ctggcttcca gaacgcctgg gccgagatct cggcgcaagc aagcgctgct      480

gtagcaaaag ctcgcaaggc gaacccttcc ttcaaggtcg tcgccacagg ccactccctc      540

ggcggcgctg tggccacact gagtgctgca aaccttcgag ctgctggtac acccgtcgac      600

atctacacat atggtgctcc tcgagtagga aacgccgcgc tctctgcttt catctcgaac      660

caggctggcg gagaatttcg cgttacgcac gacaaggatc ccgtgcctcg tcttccccct      720

ctgatcttcg gataccgaca cacaacccca gagtactggc tgtctggcgg cggcggcgac      780

aaggttgact acgccatcag cgacgtcaag gtctgtgagg gtgctgccaa tctcatgtgc      840

aacggtggaa ctctgggtct ggatattgat gctcatctgc actacttcca ggcgactgat      900

gcttgcaacg ctggtggctt ctcttggaga tcttatagga gcgccaagcg tgagagcatc      960

gacatgaggg ctaccatgac agacgcacag ttggaggcca agctcaactc ttatgttgcc     1020

atggatcagg agtatgtcaa gactcaccaa aaccgcacat gaggcgcgcc                1070


<210>  27
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
ctgatatcct ggcatggtga atctccgtg                                         29


<210>  28
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
catggcgcgc cgaggcagat aggcggacga ag                                     32


<210>  29
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
catggcgcgc cgtgtaagtg cgtggctgca g                                      31


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
ctgatatcga tcgagtcgaa ctgtcgcttc                                        30


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
gtttcgcatg gcgcgcctga gacaatgg                                          28


<210>  32
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
cacaggcgcg ccgatcgcca tcccgtcgcg tc                                     32


<210>  33
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
ctatgacatg ccctgaggcg atgctggcca ggtacgagct g                           41


<210>  34
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
cagcctcgcg gtcacagtga gaggaacggg gtgaagtcgt ataag                       45


<210>  35
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
ctagcgatcg cgtgtgcaca taggtgagtt ctcc                                   34


<210>  36
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
ctagcgatcg cgcagactgg catgcctcaa tcac                                   34


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
ctcgccatct gacaacctac aaatc                                             25


<210>  38
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
ctagtaccct gagttgtctc gcctcc                                            26


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
cctctaccat aacaggatcc atctg                                             25


<210>  40
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
cgtgagctga tgaaggagag aacaaagg                                          28


