                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals S.A.
       ID Biomedical  Corporation of Quebec
       BAUDOUX, Guy  J-M
       BLAIS, Normand
       Rheault, Patrick
       RUELLE, Jean-Louis
       CYR, Sonya
 
<120>  Recombinant RSV Antigens

<130>  VU62788-1 PCT

<150>  61/219,964
<151>  2009-06-24

<150>  61/334,568
<151>  2010-05-13

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  1697
<212>  DNA
<213>  respiratory syncytial virus

<400>  1
atggagttgc taatcctcaa agcaaatgca attaccacaa tcctcactgc agtcacattt       60

gttttgcttc tggtcaaaac atcactgaag aattttatca atcaacatgc agtgcagtag      120

caaaggctat cttagtgctc tgagaactgg ttggtatacc agtgttataa ctatagatta      180

agtaatatca aggaaaataa gtgtaatgga acagatgcta aggtaaaatt gataaacaag      240

aattagataa atataaaaat gctgtaacag aattgcagtt gctcatgcaa agcacccagc      300

aacaaacaat cgagccagaa gagaactacc aaggtttatg aattatacac tcaaaatgcc      360

aaaaaaacca atgtaacatt aagcaagaaa aggaaaagaa gatttcttgg tttttgttag      420

gtgttggatc tgcaatcgcc agtggcgttg ctgtatctaa ggtcctgcac ctgaagggga      480

agtgaacaag atcaaaagtg ctctactatc cacaaacaag gctgtagtca gttatcaaat      540

ggagttagtg tcttaaccag caaagtgtta gacctcaaaa actatataga aaacaattgt      600

tacctattgt gaacaagcaa agctgcagca tatcaaatat agcaactgta tagagttcca      660

acaaaagaac aacagactac tagagattac cagggaattt agtgttaagc aggtgtaact      720

acacctgtaa gcacttacat gttaactaat agtgaattat tgtcattatc aatgatatgc      780

ctataacaaa tgatcagaaa aagttaatgt ccaacaatgt tcaaatgtta gacagcaaag      840

ttactctatc atgtccataa taaaagagga agtcttagca tatgtgtaca attaccacta      900

tatggtgtta tagatacacc ctgttggaaa ctacacacat ccccctatgt acaaccaaca      960

caaaagaagg gtccaacatc tgtttaacaa gaactgacag aggtggtact gtgacaatgc     1020

aggatcagta tctttcttcc cacaagctga aacatgtaaa gtcaatcaaa tcgagtattt     1080

tgtgacacaa tgaacagttt aacattacca agtgaagtaa actctgcaat gttgacatat     1140

tcaaccccaa atatgattgt aaaattatga cttcaaaaac gatgtaagca gctccgttat     1200

cacatctcta ggagccattg tgtcatgcta tggcaaaaca aatgtacagc atccaataaa     1260

aatcgtggaa tcataaagac attttctaac gggtgcgata tgtatcaaat aaaggggtgg     1320

acactgtgtc tgtaggtaac acattatatt atgtaaaaag caagaaggta aaagtctcta     1380

tgtaaaaggt gaaccaataa taaatttcta tgacccttag tattcccctc tgatgaattt     1440

gatgcatcaa tatctcaagt caacgagaag attaacagag cctagcattt attcgtaaat     1500

ccgatgaatt attacataat gtaaatgctg gtaatccacc ataaatatca tgataactac     1560

tataattata gtgattatag taatattgtt atcttaattg ctgttggact gctcttatac     1620

tgtaaggcca gaagcacacc agtcacacta agaaagatca actgagtggt ataaataata     1680

ttgcatttag taactaa                                                    1697


<210>  2
<211>  574
<212>  PRT
<213>  respiratory syncytial virus

<400>  2

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Ala Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu His Asn Val Asn Ala Gly Lys Ser Thr Ile Asn Ile Met Ile Thr 
        515                 520                 525             


Thr Ile Ile Ile Val Ile Ile Val Ile Leu Leu Ser Leu Ile Ala Val 
    530                 535                 540                 


Gly Leu Leu Leu Tyr Cys Lys Ala Arg Ser Thr Pro Val Thr Leu Ser 
545                 550                 555                 560 


Lys Asp Gln Leu Ser Gly Ile Asn Asn Ile Ala Phe Ser Asn 
                565                 570                 


<210>  3
<211>  897
<212>  DNA
<213>  respiratory syncytial virus

<400>  3
atgtccaaaa acaaggacca acgcaccgct aagacactag aaaagacctg ggacactctc       60

aatcatttat tattcatatc atcgggctta tataagttaa atcttaaatc tatagcacaa      120

atcacattat ccattctggc aatgataatc tcaacttcac ttataattac agccatcata      180

ttcatagcct cggcaaacca caaagtcaca ctaacaactg caatcataca agatgcaaca      240

agccagatca agaacacaac cccaacatac ctcactcagg atcctcagct tggaatcagc      300

ttctccaatc tgtctgaaat tacatcacaa accaccacca tactagcttc aacaacacca      360

ggagtcaagt caaacctgca acccacaaca gtcaagacta aaaacacaac aacaacccaa      420

acacaaccca gcaagcccac tacaaaacaa cgccaaaaca aaccaccaaa caaacccaat      480

aatgattttc acttcgaagt gtttaacttt gtaccctgca gcatatgcag caacaatcca      540

acctgctggg ctatctgcaa aagaatacca aacaaaaaac caggaaagaa aaccaccacc      600

aagcctacaa aaaaaccaac cttcaagaca accaaaaaag atctcaaacc tcaaaccact      660

aaaccaaagg aagtacccac caccaagccc acagaagagc caaccatcaa caccaccaaa      720

acaaacatca caactacact gctcaccaac aacaccacag gaaatccaaa actcacaagt      780

caaatggaaa ccttccactc aacctcctcc gaaggcaatc taagcccttc tcaagtctcc      840

acaacatccg agcacccatc acaaccctca tctccaccca acacaacacg ccagtag         897


<210>  4
<211>  298
<212>  PRT
<213>  respiratory syncytial virus

<400>  4

Met Ser Lys Asn Lys Asp Gln Arg Thr Ala Lys Thr Leu Glu Lys Thr 
1               5                   10                  15      


Trp Asp Thr Leu Asn His Leu Leu Phe Ile Ser Ser Gly Leu Tyr Lys 
            20                  25                  30          


Leu Asn Leu Lys Ser Ile Ala Gln Ile Thr Leu Ser Ile Leu Ala Met 
        35                  40                  45              


Ile Ile Ser Thr Ser Leu Ile Ile Thr Ala Ile Ile Phe Ile Ala Ser 
    50                  55                  60                  


Ala Asn His Lys Val Thr Leu Thr Thr Ala Ile Ile Gln Asp Ala Thr 
65                  70                  75                  80  


Ser Gln Ile Lys Asn Thr Thr Pro Thr Tyr Leu Thr Gln Asp Pro Gln 
                85                  90                  95      


Leu Gly Ile Ser Phe Ser Asn Leu Ser Glu Ile Thr Ser Gln Thr Thr 
            100                 105                 110         


Thr Ile Leu Ala Ser Thr Thr Pro Gly Val Lys Ser Asn Leu Gln Pro 
        115                 120                 125             


Thr Thr Val Lys Thr Lys Asn Thr Thr Thr Thr Gln Thr Gln Pro Ser 
    130                 135                 140                 


Lys Pro Thr Thr Lys Gln Arg Gln Asn Lys Pro Pro Asn Lys Pro Asn 
145                 150                 155                 160 


Asn Asp Phe His Phe Glu Val Phe Asn Phe Val Pro Cys Ser Ile Cys 
                165                 170                 175     


Ser Asn Asn Pro Thr Cys Trp Ala Ile Cys Lys Arg Ile Pro Asn Lys 
            180                 185                 190         


Lys Pro Gly Lys Lys Thr Thr Thr Lys Pro Thr Lys Lys Pro Thr Phe 
        195                 200                 205             


Lys Thr Thr Lys Lys Asp Leu Lys Pro Gln Thr Thr Lys Pro Lys Glu 
    210                 215                 220                 


Val Pro Thr Thr Lys Pro Thr Glu Glu Pro Thr Ile Asn Thr Thr Lys 
225                 230                 235                 240 


Thr Asn Ile Thr Thr Thr Leu Leu Thr Asn Asn Thr Thr Gly Asn Pro 
                245                 250                 255     


Lys Leu Thr Ser Gln Met Glu Thr Phe His Ser Thr Ser Ser Glu Gly 
            260                 265                 270         


Asn Leu Ser Pro Ser Gln Val Ser Thr Thr Ser Glu His Pro Ser Gln 
        275                 280                 285             


Pro Ser Ser Pro Pro Asn Thr Thr Arg Gln 
    290                 295             


<210>  5
<211>  1566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant PreF polynucleotide

<400>  5
aagcttgcca ccatggagct gctgatcctg aaaaccaacg ccatcaccgc catcctggcc       60

gccgtgaccc tgtgcttcgc ctcctcccag aacatcaccg aggagttcta ccagtccacc      120

tgctccgccg tgtccaaggg ctacctgtcc gccctgcgga ccggctggta cacctccgtg      180

atcaccatcg agctgtccaa catcaaggaa aacaagtgca acggcaccga cgccaaggtg      240

aagctgatca agcaggagct ggacaagtac aagagcgccg tgaccgaact ccagctgctg      300

atgcagtcca cccctgccac caacaacaag tttctgggct tcctgctggg cgtgggctcc      360

gccatcgcct ccggcatcgc cgtgagcaag gtgctgcacc tggagggcga ggtgaacaag      420

atcaagagcg ccctgctgtc caccaacaag gccgtggtgt ccctgtccaa cggcgtgtcc      480

gtgctgacct ccaaggtgct ggatctgaag aactacatcg acaagcagct gctgcctatc      540

gtgaacaagc agtcctgctc catctccaac atcgagaccg tgatcgagtt ccagcagaag      600

aacaaccggc tgctggagat cacccgcgag ttctccgtga acgccggcgt gaccacccct      660

gtgtccacct acatgctgac caactccgag ctgctgtccc tgatcaacga catgcctatc      720

accaacgacc agaaaaaact gatgtccaac aacgtgcaga tcgtgcggca gcagtcctac      780

agcatcatga gcatcatcaa ggaagaggtg ctggcctacg tggtgcagct gcctctgtac      840

ggcgtgatcg acaccccttg ctggaagctg cacacctccc ccctgtgcac caccaacacc      900

aaggagggct ccaacatctg cctgacccgg accgaccggg gctggtactg cgacaacgcc      960

ggctccgtgt ccttcttccc tctggccgag acctgcaagg tgcagtccaa ccgggtgttc     1020

tgcgacacca tgaactccct gaccctgcct tccgaggtga acctgtgcaa catcgacatc     1080

ttcaacccca agtacgactg caagatcatg accagcaaga ccgacgtgtc ctccagcgtg     1140

atcacctccc tgggcgccat cgtgtcctgc tacggcaaga ccaagtgcac cgcctccaac     1200

aagaaccggg gaatcatcaa gaccttctcc aacggctgcg actacgtgtc caataagggc     1260

gtggacaccg tgtccgtggg caacacactg tactacgtga ataagcagga gggcaagagc     1320

ctgtacgtga agggcgagcc tatcatcaac ttctacgacc ctctggtgtt cccttccgac     1380

gagttcgacg cctccatcag ccaggtgaac gagaagatca accagtccct ggccttcatc     1440

cggaagtccg acgagaagct gcataacgtg gaggacaaga tcgaggagat cctgtccaaa     1500

atctaccaca tcgagaacga gatcgcccgg atcaagaagc tgatcggcga ggcctgataa     1560

tctaga                                                                1566


<210>  6
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant PreF Antigen

<400>  6

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Phe Leu Gly Phe Leu Leu 
            100                 105                 110         


Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val Leu 
        115                 120                 125             


His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr 
    130                 135                 140                 


Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser 
145                 150                 155                 160 


Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile 
                165                 170                 175     


Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu 
            180                 185                 190         


Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser 
        195                 200                 205             


Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn 
    210                 215                 220                 


Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln 
225                 230                 235                 240 


Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr 
                245                 250                 255     


Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln 
            260                 265                 270         


Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr 
        275                 280                 285             


Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu 
    290                 295                 300                 


Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser 
305                 310                 315                 320 


Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe 
                325                 330                 335     


Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys 
            340                 345                 350         


Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser 
        355                 360                 365             


Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val 
    370                 375                 380                 


Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly 
385                 390                 395                 400 


Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly 
                405                 410                 415     


Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln 
            420                 425                 430         


Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr 
        435                 440                 445             


Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln 
    450                 455                 460                 


Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 
                485                 490                 495     


Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 
            500                 505                 510         


Glu Ala 
        


<210>  7
<211>  1855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric PreF-G Antigen polynucleotide seqeunce

<400>  7
aagcttgcca ccatggagct gctgatcctc aagaccaacg ccatcaccgc catcctggcc       60

gccgtgaccc tgtgcttcgc ctcctcccag aacatcaccg aagagttcta ccagtccacc      120

tgctccgccg tgtccaaggg ctacctgtcc gccctgcgga ccggctggta cacctccgtg      180

atcaccatcg agctgtccaa catcaaagaa aacaagtgca acggcaccga cgccaaggtc      240

aagctgatca agcaggaact ggacaagtac aagagcgccg tgaccgaact ccagctgctg      300

atgcagtcca cccctgccac caacaacaag aagtttctgg gcttcctgct gggcgtgggc      360

tccgccatcg cctccggcat cgccgtgagc aaggtgctgc acctggaggg cgaggtgaac      420

aagatcaaga gcgccctgct gtccaccaac aaggccgtgg tgtccctgtc caacggcgtg      480

tccgtgctga cctccaaggt gctggatctg aagaactaca tcgacaagca gctgctgcct      540

atcgtgaaca agcagtcctg ctccatctcc aacatcgaga ccgtgatcga gttccagcag      600

aagaacaacc ggctgctgga gatcacccgc gagttctccg tgaacgccgg cgtgaccacc      660

cctgtgtcca cctacatgct gacaaactcc gagctgctct ccctgatcaa cgacatgcct      720

atcaccaacg accaaaaaaa gctgatgtcc aacaacgtgc agatcgtgcg gcagcagtcc      780

tacagcatca tgagcatcat caaggaagaa gtcctggcct acgtcgtgca gctgcctctg      840

tacggcgtga tcgacacccc ttgctggaag ctgcacacct cccccctgtg caccaccaac      900

accaaagagg gctccaacat ctgcctgacc cggaccgacc ggggctggta ctgcgacaac      960

gccggctccg tgtccttctt ccctctggcc gagacctgca aggtgcagtc caaccgggtg     1020

ttctgcgaca ccatgaactc cctgaccctg ccttccgagg tgaacctgtg caacatcgac     1080

atcttcaacc ccaagtacga ctgcaagatc atgaccagca agaccgacgt gtcctccagc     1140

gtgatcacct ccctgggcgc catcgtgtcc tgctacggca agaccaagtg caccgcctcc     1200

aacaagaacc ggggaatcat caagaccttc tccaacggct gcgactacgt gtccaataag     1260

ggcgtggaca ccgtgtccgt gggcaacaca ctgtactacg tgaataagca ggaaggcaag     1320

agcctgtacg tgaagggcga gcctatcatc aacttctacg accctctggt gttcccttcc     1380

gacgagttcg acgcctccat cagccaggtc aacgagaaga tcaaccagtc cctggccttc     1440

atccggaagt ccgacgagaa gctgcataac gtggaggaca agatcgaaga gatcctgtcc     1500

aaaatctacc acatcgagaa cgagatcgcc cggatcaaga agctgatcgg cgaggctggc     1560

ggctctggcg gcagcggcgg ctccaagcag cggcagaaca agcctcctaa caagcccaac     1620

aacgacttcc acttcgaggt gttcaacttc gtgccttgct ccatctgctc caacaaccct     1680

acctgctggg ccatctgcaa gagaatcccc aacaagaagc ctggcaagaa aaccaccacc     1740

aagcctacca agaagcctac cttcaagacc accaagaagg accacaagcc tcagaccaca     1800

aagcctaagg aagtgccaac caccaagcac caccaccatc accactgata atcta          1855


<210>  8
<211>  605
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric PreF-G polypeptide

<400>  8

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Lys Phe Leu Gly Phe Leu 
            100                 105                 110         


Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val 
        115                 120                 125             


Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser 
    130                 135                 140                 


Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr 
145                 150                 155                 160 


Ser Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro 
                165                 170                 175     


Ile Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile 
            180                 185                 190         


Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe 
        195                 200                 205             


Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr 
    210                 215                 220                 


Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp 
225                 230                 235                 240 


Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser 
                245                 250                 255     


Tyr Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val 
            260                 265                 270         


Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His 
        275                 280                 285             


Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys 
    290                 295                 300                 


Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val 
305                 310                 315                 320 


Ser Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val 
                325                 330                 335     


Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu 
            340                 345                 350         


Cys Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr 
        355                 360                 365             


Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile 
    370                 375                 380                 


Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg 
385                 390                 395                 400 


Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys 
                405                 410                 415     


Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys 
            420                 425                 430         


Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe 
        435                 440                 445             


Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser 
    450                 455                 460                 


Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser 
465                 470                 475                 480 


Asp Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser 
                485                 490                 495     


Lys Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile 
            500                 505                 510         


Gly Glu Ala Gly Gly Ser Gly Gly Ser Gly Gly Ser Lys Gln Arg Gln 
        515                 520                 525             


Asn Lys Pro Pro Asn Lys Pro Asn Asn Asp Phe His Phe Glu Val Phe 
    530                 535                 540                 


Asn Phe Val Pro Cys Ser Ile Cys Ser Asn Asn Pro Thr Cys Trp Ala 
545                 550                 555                 560 


Ile Cys Lys Arg Ile Pro Asn Lys Lys Pro Gly Lys Lys Thr Thr Thr 
                565                 570                 575     


Lys Pro Thr Lys Lys Pro Thr Phe Lys Thr Thr Lys Lys Asp His Lys 
            580                 585                 590         


Pro Gln Thr Thr Lys Pro Lys Glu Val Pro Thr Thr Lys 
        595                 600                 605 


<210>  9
<211>  1834
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric PreF-G polynucleotide

<400>  9
aagcttgcca ccatggagct gctgatcctc aagaccaacg ccatcaccgc catcctggcc       60

gccgtgaccc tgtgcttcgc ctcctcccag aacatcaccg aagagttcta ccagtccacc      120

tgctccgccg tgtccaaggg ctacctgtcc gccctgcgga ccggctggta cacctccgtg      180

atcaccatcg agctgtccaa catcaaagaa aacaagtgca acggcaccga cgccaaggtc      240

aagctgatca agcaggaact ggacaagtac aagagcgccg tgaccgaact ccagctgctg      300

atgcagtcca cccctgccac caacaacaag aagtttctgg gcttcctgct gggcgtgggc      360

tccgccatcg cctccggcat cgccgtgagc aaggtgctgc acctggaggg cgaggtgaac      420

aagatcaaga gcgccctgct gtccaccaac aaggccgtgg tgtccctgtc caacggcgtg      480

tccgtgctga cctccaaggt gctggatctg aagaactaca tcgacaagca gctgctgcct      540

atcgtgaaca agcagtcctg ctccatctcc aacatcgaga ccgtgatcga gttccagcag      600

aagaacaacc ggctgctgga gatcacccgc gagttctccg tgaacgccgg cgtgaccacc      660

cctgtgtcca cctacatgct gacaaactcc gagctgctct ccctgatcaa cgacatgcct      720

atcaccaacg accaaaaaaa gctgatgtcc aacaacgtgc agatcgtgcg gcagcagtcc      780

tacagcatca tgagcatcat caaggaagaa gtcctggcct acgtcgtgca gctgcctctg      840

tacggcgtga tcgacacccc ttgctggaag ctgcacacct cccccctgtg caccaccaac      900

accaaagagg gctccaacat ctgcctgacc cggaccgacc ggggctggta ctgcgacaac      960

gccggctccg tgtccttctt ccctctggcc gagacctgca aggtgcagtc caaccgggtg     1020

ttctgcgaca ccatgaactc cctgaccctg ccttccgagg tgaacctgtg caacatcgac     1080

atcttcaacc ccaagtacga ctgcaagatc atgaccagca agaccgacgt gtcctccagc     1140

gtgatcacct ccctgggcgc catcgtgtcc tgctacggca agaccaagtg caccgcctcc     1200

aacaagaacc ggggaatcat caagaccttc tccaacggct gcgactacgt gtccaataag     1260

ggcgtggaca ccgtgtccgt gggcaacaca ctgtactacg tgaataagca ggaaggcaag     1320

agcctgtacg tgaagggcga gcctatcatc aacttctacg accctctggt gttcccttcc     1380

gacgagttcg acgcctccat cagccaggtc aacgagaaga tcaaccagtc cctggccttc     1440

atccggaagt ccgacgagaa gctgcataac gtggaggaca agatcgaaga gatcctgtcc     1500

aaaatctacc acatcgagaa cgagatcgcc cggatcaaga agctgatcgg cgaggctggc     1560

ggcaagcagc ggcagaacaa gcctcctaac aagcccaaca acgacttcca cttcgaggtg     1620

ttcaacttcg tgccttgctc catctgctcc aacaacccta cctgctgggc catctgcaag     1680

agaatcccca acaagaagcc tggcaagaaa accaccacca agcctaccaa gaagcctacc     1740

ttcaagacca ccaagaagga ccacaagcct cagaccacaa agcctaagga agtgccaacc     1800

accaagcacc accaccatca ccactgataa tcta                                 1834


<210>  10
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric PreF-G polypeptide

<400>  10

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Lys Phe Leu Gly Phe Leu 
            100                 105                 110         


Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val 
        115                 120                 125             


Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser 
    130                 135                 140                 


Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr 
145                 150                 155                 160 


Ser Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro 
                165                 170                 175     


Ile Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile 
            180                 185                 190         


Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe 
        195                 200                 205             


Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr 
    210                 215                 220                 


Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp 
225                 230                 235                 240 


Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser 
                245                 250                 255     


Tyr Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val 
            260                 265                 270         


Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His 
        275                 280                 285             


Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys 
    290                 295                 300                 


Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val 
305                 310                 315                 320 


Ser Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val 
                325                 330                 335     


Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu 
            340                 345                 350         


Cys Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr 
        355                 360                 365             


Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile 
    370                 375                 380                 


Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg 
385                 390                 395                 400 


Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys 
                405                 410                 415     


Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys 
            420                 425                 430         


Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe 
        435                 440                 445             


Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser 
    450                 455                 460                 


Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser 
465                 470                 475                 480 


Asp Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser 
                485                 490                 495     


Lys Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile 
            500                 505                 510         


Gly Glu Ala Gly Gly Lys Gln Arg Gln Asn Lys Pro Pro Asn Lys Pro 
        515                 520                 525             


Asn Asn Asp Phe His Phe Glu Val Phe Asn Phe Val Pro Cys Ser Ile 
    530                 535                 540                 


Cys Ser Asn Asn Pro Thr Cys Trp Ala Ile Cys Lys Arg Ile Pro Asn 
545                 550                 555                 560 


Lys Lys Pro Gly Lys Lys Thr Thr Thr Lys Pro Thr Lys Lys Pro Thr 
                565                 570                 575     


Phe Lys Thr Thr Lys Lys Asp His Lys Pro Gln Thr Thr Lys Pro Lys 
            580                 585                 590         


Glu Val Pro Thr Thr Lys 
        595             


<210>  11
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Isoleucine substituted GCN4 leucine zipper

<400>  11

Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys Ile Tyr His Ile Glu Asn 
1               5                   10                  15      


Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly Glu Ala 
            20                  25              


<210>  12
<211>  1563
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon optimized PreF nucleotide sequence

<400>  12
atggagctgc ccatcctgaa gaccaacgcc atcaccacca tcctcgccgc cgtgaccctg       60

tgcttcgcca gcagccagaa catcacggag gagttctacc agagcacgtg cagcgccgtg      120

agcaagggct acctgagcgc gctgcgcacg ggctggtaca cgagcgtgat cacgatcgag      180

ctgagcaaca tcaaggagaa caagtgcaac ggcacggacg cgaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gagcgcggtg acggagctgc agctgctgat gcagagcacg      300

ccggcgacga acaacaagtt cctcggcttc ctgctgggcg tgggcagcgc gatcgcgagc      360

ggcatcgccg tgagcaaggt gctgcacctg gagggcgagg tgaacaagat caagtccgcg      420

ctgctgagca cgaacaaggc ggtcgtgagc ctgagcaacg gcgtgagcgt gctgacgagc      480

aaggtgctcg acctgaagaa ctacatcgac aagcagctgc tgccgatcgt gaacaagcag      540

agctgcagca tcagcaacat cgagaccgtg atcgagttcc agcagaagaa caaccgcctg      600

ctggagatca cgcgggagtt ctccgtgaac gcaggcgtga cgacgcccgt gtctacgtac      660

atgctgacga acagcgagct gctcagcctg atcaacgaca tgccgatcac gaacgaccag      720

aagaagctga tgagcaacaa cgtgcagatc gtgcgccagc agagctacag catcatgagc      780

atcatcaagg aggaggtgct ggcatacgtg gtgcagctgc cgctgtacgg cgtcatcgac      840

acgccctgct ggaagctgca cacgagcccg ctgtgcacga ccaacacgaa ggagggcagc      900

aacatctgcc tgacgcggac ggaccggggc tggtactgcg acaacgcggg cagcgtgagc      960

ttcttcccgc tcgcggagac gtgcaaggtg cagagcaacc gcgtcttctg cgacacgatg     1020

aacagcctga cgctgccgag cgaggtgaac ctgtgcaaca tcgacatctt caacccgaag     1080

tacgactgca agatcatgac gagcaagacc gatgtcagca gcagcgtgat cacgagcctc     1140

ggcgcgatcg tgagctgcta cggcaagacg aagtgcacgg cgagcaacaa gaaccgcggc     1200

atcatcaaga cgttcagcaa cggctgcgac tatgtgagca acaagggcgt ggacactgtg     1260

agcgtcggca acacgctgta ctacgtgaac aagcaggagg gcaagagcct gtacgtgaag     1320

ggcgagccga tcatcaactt ctacgacccg ctcgtgttcc cgagcgacga gttcgacgcg     1380

agcatcagcc aagtgaacga gaagatcaac cagagcctgg cgttcatccg caagagcgac     1440

gagaagctgc acaacgtgga ggacaagatc gaggagatcc tgagcaagat ctaccacatc     1500

gagaacgaga tcgcgcgcat caagaagctg atcggcgagg cgcatcatca ccatcaccat     1560

tga                                                                   1563


<210>  13
<211>  1685
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PreF polynucleotide sequence with intron

<400>  13
atggagctgc tgatcctgaa aaccaacgcc atcaccgcca tcctggccgc cgtgaccctg       60

tgcttcgcct cctcccagaa catcaccgag gagttctacc agtccacctg ctccgccgtg      120

tccaagggct acctgtccgc cctgcggacc ggctggtaca cctccgtgat caccatcgag      180

ctgtccaaca tcaaggaaaa caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gagcgccgtg accgaactcc agctgctgat gcagtccacc      300

cctgccacca acaacaagtt tctgggcttc ctgctgggcg tgggctccgc catcgcctcc      360

ggcatcgccg tgagcaaggt acgtgtcggg acttgtgttc cccttttttt aataaaaagt      420

tatatcttta atgttatata catatttcct gtatgtgatc catgtgctta tgactttgtt      480

tatcatgtgt ttaggtgctg cacctggagg gcgaggtgaa caagatcaag agcgccctgc      540

tgtccaccaa caaggccgtg gtgtccctgt ccaacggcgt gtccgtgctg acctccaagg      600

tgctggatct gaagaactac atcgacaagc agctgctgcc tatcgtgaac aagcagtcct      660

gctccatctc caacatcgag accgtgatcg agttccagca gaagaacaac cggctgctgg      720

agatcacccg cgagttctcc gtgaacgccg gcgtgaccac ccctgtgtcc acctacatgc      780

tgaccaactc cgagctgctg tccctgatca acgacatgcc tatcaccaac gaccagaaaa      840

aactgatgtc caacaacgtg cagatcgtgc ggcagcagtc ctacagcatc atgagcatca      900

tcaaggaaga ggtgctggcc tacgtggtgc agctgcctct gtacggcgtg atcgacaccc      960

cttgctggaa gctgcacacc tcccccctgt gcaccaccaa caccaaggag ggctccaaca     1020

tctgcctgac ccggaccgac cggggctggt actgcgacaa cgccggctcc gtgtccttct     1080

tccctctggc cgagacctgc aaggtgcagt ccaaccgggt gttctgcgac accatgaact     1140

ccctgaccct gccttccgag gtgaacctgt gcaacatcga catcttcaac cccaagtacg     1200

actgcaagat catgaccagc aagaccgacg tgtcctccag cgtgatcacc tccctgggcg     1260

ccatcgtgtc ctgctacggc aagaccaagt gcaccgcctc caacaagaac cggggaatca     1320

tcaagacctt ctccaacggc tgcgactacg tgtccaataa gggcgtggac accgtgtccg     1380

tgggcaacac actgtactac gtgaataagc aggagggcaa gagcctgtac gtgaagggcg     1440

agcctatcat caacttctac gaccctctgg tgttcccttc cgacgagttc gacgcctcca     1500

tcagccaggt gaacgagaag atcaaccagt ccctggcctt catccggaag tccgacgaga     1560

agctgcataa cgtggaggac aagatcgagg agatcctgtc caaaatctac cacatcgaga     1620

acgagatcgc ccggatcaag aagctgatcg gcgaggccgg aggtcaccac caccatcacc     1680

actga                                                                 1685


<210>  14
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic linker peptide

<400>  14

Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   


<210>  15
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Furin cleavage consensus motif

<400>  15

Arg Ala Arg Arg 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Furin cleavage consensus motif

<400>  16

Arg Lys Arg Arg 
1               


<210>  17
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polynucleotide

<400>  17
atggagctgc tgatcctgaa aaccaacgcc atcaccgcca tcctggccgc cgtgaccctg       60

tgcttcgcct cctcccagaa catcaccgag gagttctacc agtccacctg ctccgccgtg      120

tccaagggct acctgtccgc cctgcggacc ggctggtaca cctccgtgat caccatcgag      180

ctgtccaaca tcaaggaaaa caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gagcgccgtg accgaactcc agctgctgat gcagtccacc      300

cctgccacca acaacaagtt tctgggcttc ctgctgggcg tgggctccgc catcgcctcc      360

ggcatcgccg tgagcaaggt gctgcacctg gagggcgagg tgaacaagat caagagcgcc      420

ctgctgtcca ccaacaaggc cgtggtgtcc ctgtccaacg gcgtgtccgt gctgacctcc      480

aaggtgctgg atctgaagaa ctacatcgac aagcagctgc tgcctatcgt gaacaagcag      540

tcctgctcca tctccaacat cgagaccgtg atcgagttcc agcagaagaa caaccggctg      600

ctggagatca cccgcgagtt ctccgtgaac gccggcgtga ccacccctgt gtccacctac      660

atgctgacca actccgagct gctgtccctg atcaacgaca tgcctatcac caacgaccag      720

aaaaaactga tgtccaacaa cgtgcagatc gtgcggcagc agtcctacag catcatgagc      780

atcatcaagg aagaggtgct ggcctacgtg gtgcagctgc ctctgtacgg cgtgatcgac      840

accccttgct ggaagctgca cacctccccc ctgtgcacca ccaacaccaa ggagggctcc      900

aacatctgcc tgacccggac cgaccggggc tggtactgcg acaacgccgg ctccgtgtcc      960

ttcttccctc tggccgagac ctgcaaggtg cagtccaacc gggtgttctg cgacaccatg     1020

aactccctga ccctgccttc cgaggtgaac ctgtgcaaca tcgacatctt caaccccaag     1080

tacgactgca agatcatgac cagcaagacc gacgtgtcct ccagcgtgat cacctccctg     1140

ggcgccatcg tgtcctgcta cggcaagacc aagtgcaccg cctccaacaa gaaccgggga     1200

atcatcaaga ccttctccaa cggctgcgac tacgtgtcca ataagggcgt ggacaccgtg     1260

tccgtgggca acacactgta ctacgtgaat aagcaggagg gcaagagcct gtacgtgaag     1320

ggcgagccta tcatcaactt ctacgaccct ctggtgttcc cttccgacga gttcgacgcc     1380

tccatcagcc aggtgaacga gaagatcaac gggaccctgg ccttcatccg gaagtccgac     1440

gagaagctgc ataacgtgga ggacaagatc gaggagatcc tgtccaaaat ctaccacatc     1500

gagaacgaga tcgcccggat caagaagctg atcggcgagg cc                        1542


<210>  18
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polypeptide

<400>  18

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Phe Leu Gly Phe Leu Leu 
            100                 105                 110         


Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val Leu 
        115                 120                 125             


His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr 
    130                 135                 140                 


Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser 
145                 150                 155                 160 


Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile 
                165                 170                 175     


Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu 
            180                 185                 190         


Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser 
        195                 200                 205             


Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn 
    210                 215                 220                 


Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln 
225                 230                 235                 240 


Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr 
                245                 250                 255     


Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln 
            260                 265                 270         


Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr 
        275                 280                 285             


Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu 
    290                 295                 300                 


Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser 
305                 310                 315                 320 


Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe 
                325                 330                 335     


Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys 
            340                 345                 350         


Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser 
        355                 360                 365             


Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val 
    370                 375                 380                 


Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly 
385                 390                 395                 400 


Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly 
                405                 410                 415     


Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln 
            420                 425                 430         


Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr 
        435                 440                 445             


Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln 
    450                 455                 460                 


Val Asn Glu Lys Ile Asn Gly Thr Leu Ala Phe Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 
                485                 490                 495     


Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 
            500                 505                 510         


Glu Ala 
        


<210>  19
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polynucleotide

<400>  19
atggagctgc tgatcctgaa aaccaacgcc atcaccgcca tcctggccgc cgtgaccctg       60

tgcttcgcct cctcccagaa catcaccgag gagttctacc agtccacctg ctccgccgtg      120

tccaagggct acctgtccgc cctgcggacc ggctggtaca cctccgtgat caccatcgag      180

ctgtccaaca tcaaggaaaa caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gagcgccgtg accgaactcc agctgctgat gcagtccacc      300

cctgccacca acaacaagtt tctgggcttc ctgcagggcg tgggctccgc catcgcctcc      360

ggcatcgccg tgagcaaggt gctgcacctg gagggcgagg tgaacaagat caagagcgcc      420

ctgctgtcca ccaacaaggc cgtggtgtcc ctgtccaacg gcgtgtccgt gctgacctcc      480

aaggtgctgg atctgaagaa ctacatcgac aagcagctgc tgcctatcgt gaacaagcag      540

tcctgctcca tctccaacat cgagaccgtg atcgagttcc agcagaagaa caaccggctg      600

ctggagatca cccgcgagtt ctccgtgaac gccggcgtga ccacccctgt gtccacctac      660

atgctgacca actccgagct gctgtccctg atcaacgaca tgcctatcac caacgaccag      720

aaaaaactga tgtccaacaa cgtgcagatc gtgcggcagc agtcctacag catcatgagc      780

atcatcaagg aagaggtgct ggcctacgtg gtgcagctgc ctctgtacgg cgtgatcgac      840

accccttgct ggaagctgca cacctccccc ctgtgcacca ccaacaccaa ggagggctcc      900

aacatctgcc tgacccggac cgaccggggc tggtactgcg acaacgccgg ctccgtgtcc      960

ttcttccctc tggccgagac ctgcaaggtg cagtccaacc gggtgttctg cgacaccatg     1020

aactccctga ccctgccttc cgaggtgaac ctgtgcaaca tcgacatctt caaccccaag     1080

tacgactgca agatcatgac cagcaagacc gacgtgtcct ccagcgtgat cacctccctg     1140

ggcgccatcg tgtcctgcta cggcaagacc aagtgcaccg cctccaacaa gaaccgggga     1200

atcatcaaga ccttctccaa cggctgcgac tacgtgtcca ataagggcgt ggacaccgtg     1260

tccgtgggca acacactgta ctacgtgaat aagcaggagg gcaagagcct gtacgtgaag     1320

ggcgagccta tcatcaactt ctacgaccct ctggtgttcc cttccgacga gttcgacgcc     1380

tccatcagcc aggtgaacga gaagatcaac cagtccctgg ccttcatccg gaagtccgac     1440

gagaagctgc ataacgtgga ggacaagatc gaggagatcc tgtccaaaat ctaccacatc     1500

gagaacgaga tcgcccggat caagaagctg atcggcgagg cc                        1542


<210>  20
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polypeptide

<400>  20

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Phe Leu Gly Phe Leu Gln 
            100                 105                 110         


Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val Leu 
        115                 120                 125             


His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr 
    130                 135                 140                 


Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser 
145                 150                 155                 160 


Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile 
                165                 170                 175     


Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu 
            180                 185                 190         


Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser 
        195                 200                 205             


Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn 
    210                 215                 220                 


Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln 
225                 230                 235                 240 


Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr 
                245                 250                 255     


Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln 
            260                 265                 270         


Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr 
        275                 280                 285             


Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu 
    290                 295                 300                 


Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser 
305                 310                 315                 320 


Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe 
                325                 330                 335     


Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys 
            340                 345                 350         


Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser 
        355                 360                 365             


Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val 
    370                 375                 380                 


Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly 
385                 390                 395                 400 


Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly 
                405                 410                 415     


Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln 
            420                 425                 430         


Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr 
        435                 440                 445             


Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln 
    450                 455                 460                 


Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 
                485                 490                 495     


Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 
            500                 505                 510         


Glu Ala 
        


<210>  21
<211>  1542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polynucleotide

<400>  21
atggagctgc tgatcctgaa aaccaacgcc atcaccgcca tcctggccgc cgtgaccctg       60

tgcttcgcct cctcccagaa catcaccgag gagttctacc agtccacctg ctccgccgtg      120

tccaagggct acctgtccgc cctgcggacc ggctggtaca cctccgtgat caccatcgag      180

ctgtccaaca tcaaggaaaa caagtgcaac ggcaccgacg ccaaggtgaa gctgatcaag      240

caggagctgg acaagtacaa gagcgccgtg accgaactcc agctgctgat gcagtccacc      300

cctgccacca acaacaagtt tctgggcttc ctgcagggcg tgggctccgc catcgcctcc      360

ggcatcgccg tgagcaaggt gctgcacctg gagggcgagg tgaacaagat caagagcgcc      420

ctgctgtcca ccaacaaggc cgtggtgtcc ctgtccaacg gcgtgtccgt gctgacctcc      480

aaggtgctgg atctgaagaa ctacatcgac aagcagctgc tgcctatcgt gaacaagcag      540

tcctgctcca tctccaacat cgagaccgtg atcgagttcc agcagaagaa caaccggctg      600

ctggagatca cccgcgagtt ctccgtgaac gccggcgtga ccacccctgt gtccacctac      660

atgctgacca actccgagct gctgtccctg atcaacgaca tgcctatcac caacgaccag      720

aaaaaactga tgtccaacaa cgtgcagatc gtgcggcagc agtcctacag catcatgagc      780

atcatcaagg aagaggtgct ggcctacgtg gtgcagctgc ctctgtacgg cgtgatcgac      840

accccttgct ggaagctgca cacctccccc ctgtgcacca ccaacaccaa ggagggctcc      900

aacatctgcc tgacccggac cgaccggggc tggtactgcg acaacgccgg ctccgtgtcc      960

ttcttccctc tggccgagac ctgcaaggtg cagtccaacc gggtgttctg cgacaccatg     1020

aactccctga ccctgccttc cgaggtgaac ctgtgcaaca tcgacatctt caaccccaag     1080

tacgactgca agatcatgac cagcaagacc gacgtgtcct ccagcgtgat cacctccctg     1140

ggcgccatcg tgtcctgcta cggcaagacc aagtgcaccg cctccaacaa gaaccgggga     1200

atcatcaaga ccttctccaa cggctgcgac tacgtgtcca ataagggcgt ggacaccgtg     1260

tccgtgggca acacactgta ctacgtgaat aagcaggagg gcaagagcct gtacgtgaag     1320

ggcgagccta tcatcaactt ctacgaccct ctggtgttcc cttccgacga gttcgacgcc     1380

tccatcagcc aggtgaacga gaagatcaac gggaccctgg ccttcatccg gaagtccgac     1440

gagaagctgc ataacgtgga ggacaagatc gaggagatcc tgtccaaaat ctaccacatc     1500

gagaacgaga tcgcccggat caagaagctg atcggcgagg cc                        1542


<210>  22
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PreF Polypeptide

<400>  22

Met Glu Leu Leu Ile Leu Lys Thr Asn Ala Ile Thr Ala Ile Leu Ala 
1               5                   10                  15      


Ala Val Thr Leu Cys Phe Ala Ser Ser Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Ser Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Lys Phe Leu Gly Phe Leu Gln 
            100                 105                 110         


Gly Val Gly Ser Ala Ile Ala Ser Gly Ile Ala Val Ser Lys Val Leu 
        115                 120                 125             


His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr 
    130                 135                 140                 


Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser 
145                 150                 155                 160 


Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile 
                165                 170                 175     


Val Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu 
            180                 185                 190         


Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser 
        195                 200                 205             


Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn 
    210                 215                 220                 


Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln 
225                 230                 235                 240 


Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr 
                245                 250                 255     


Ser Ile Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln 
            260                 265                 270         


Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr 
        275                 280                 285             


Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu 
    290                 295                 300                 


Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser 
305                 310                 315                 320 


Phe Phe Pro Leu Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe 
                325                 330                 335     


Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys 
            340                 345                 350         


Asn Ile Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser 
        355                 360                 365             


Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val 
    370                 375                 380                 


Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly 
385                 390                 395                 400 


Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly 
                405                 410                 415     


Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln 
            420                 425                 430         


Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr 
        435                 440                 445             


Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln 
    450                 455                 460                 


Val Asn Glu Lys Ile Asn Gly Thr Leu Ala Phe Ile Arg Lys Ser Asp 
465                 470                 475                 480 


Glu Lys Leu His Asn Val Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 
                485                 490                 495     


Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 
            500                 505                 510         


Glu Ala 
        


