                         SEQUENCE LISTING

<110>  VIB VZW
       KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D
 
<120>  SCREENING FOR COMPOUNDS THAT MODULATE GPR3-MEDIATED BETA-ARRESTIN
        SIGNALING AND AMYLOID BETA PEPTIDE GENERATION

<130>  BDS/ARR/V311

<150>  US 61/268,054
<151>  2009-06-08

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  RNA
<213>  Homo sapiens

<400>  1
aaagccuucu gcgcggagaa u                                                 21


<210>  2
<211>  21
<212>  RNA
<213>  Homo sapiens

<400>  2
aaggaccgca aaguguuugu g                                                 21


<210>  3
<211>  21
<212>  RNA
<213>  Homo sapiens

<400>  3
aaccaaccuc auugaauuug a                                                 21


<210>  4
<211>  2145
<212>  DNA
<213>  Homo sapiens

<400>  4
cgcgggggtt tctcggggtc cacgcacgcc ctgcgccgcc aggacccgag cggagcctcc       60

ccgcggcccg gccgcgcctg gtcctgagcg gtaccatgat gtggggtgca ggcagccctc      120

tggcctggct ctcagctggc tcaggcaacg tgaatgtaag cagcgtgggc ccagcagagg      180

ggcccacagg tccagccgca ccactgccct cgcctaaggc ctgggatgtg gtgctctgca      240

tctcaggcac cctggtgtcc tgcgagaatg cgctagtggt ggccatcatc gtgggcactc      300

ctgccttccg tgcccccatg ttcctgctgg tgggcagcct ggccgtggca gacctgctgg      360

caggcctggg cctggtcctg cactttgctg ctgtcttctg catcggctca gcggagatga      420

gcctggtgct ggttggcgtg ctggcaatgg cctttaccgc cagcatcggc agtctactgg      480

ccatcactgt cgaccgctac ctttctctgt acaatgccct cacctactat tcagagacaa      540

cagtgacacg gacctatgtg atgctggcct tagtgtgggg aggtgccctg ggcctggggc      600

tgctgcctgt gctggcctgg aactgcctgg atggcctgac cacatgtggc gtggtttatc      660

cactctccaa gaaccatctg gtagttctgg ccattgcctt cttcatggtg tttggcatca      720

tgctgcagct ctacgcccaa atctgccgca tcgtctgccg ccatgcccag cagattgccc      780

ttcagcggca cctgctgcct gcctcccact atgtggccac ccgcaagggc attgccacac      840

tggccgtggt gcttggagcc tttgccgcct gctggttgcc cttcactgtc tactgcctgc      900

tgggtgatgc ccactctcca cctctctaca cctatcttac cttgctccct gccacctaca      960

actccatgat caaccctatc atctacgcct tccgcaacca ggatgtgcag aaagtgctgt     1020

gggctgtctg ctgctgctgt tcctcttcca agatcccctt ccgatcccgc tcccccagtg     1080

atgtctagct gagtcttcat gacccttcaa ccctgattac tacagaattc cagaatgtta     1140

ggctctccag ggcttctttc caaaccccca gctccacacc ccccagaccc agctggttct     1200

ggagttctag gacattgggt gtttcaaggt tctgttcaga tccctatggg ggcccagctg     1260

gctccacggt tccagaatgt tcaggtggtc agtgttctac tcagaaatgt ctcacagccc     1320

agctgggttg caattccaga atgctgggag ttttacagtg ccattccaag tcccagatgt     1380

ccctcttccc ccaaacttga ccttgaccat gtcactttac gtttgaattt ctgagctaaa     1440

gagtcagaga gattagtcac atagttgcct aaataggaga gagaaagatt atatatgcac     1500

atatacaaag acagtgtcta tttatgattg atttatttat ttataaattt acttatgggt     1560

ggtaaggggc aaaaaagagg cccacacctt gatatccagg ccataccagg gtatcccttg     1620

tcccttcacc cccatttctg acctcagttc ctggaggggg gaaagggtga aagagaaacc     1680

acgtattttg ttattatttt ggattatttt ttatcgaaga gatcatagaa accagagcct     1740

tctccccagg cctgccctcc tcgggtttgg aaggggaaca caccagcctc tggtttttta     1800

tttttttaag aagccatcac ctgagcaacc aaaaattcct ctgcgctggg gtccgactgc     1860

cctctggtgg ccatttgggg aaaactgcag cccggccagg cagctgggac cagaatgcaa     1920

ccccagctcc actccagcct ggcgtccagg gccacagcca tggcctgggg gccaagcctc     1980

accctgcggt gccctaaagg agggggggca cgagccaaca ccccacccct ctgccaaccg     2040

gggtatggcc cccagtgcat tccctgttcc cgtctccaac ccaactcaat aaaaaatgat     2100

tttgtcataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                     2145


<210>  5
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Met Trp Gly Ala Gly Ser Pro Leu Ala Trp Leu Ser Ala Gly Ser 
1               5                   10                  15      


Gly Asn Val Asn Val Ser Ser Val Gly Pro Ala Glu Gly Pro Thr Gly 
            20                  25                  30          


Pro Ala Ala Pro Leu Pro Ser Pro Lys Ala Trp Asp Val Val Leu Cys 
        35                  40                  45              


Ile Ser Gly Thr Leu Val Ser Cys Glu Asn Ala Leu Val Val Ala Ile 
    50                  55                  60                  


Ile Val Gly Thr Pro Ala Phe Arg Ala Pro Met Phe Leu Leu Val Gly 
65                  70                  75                  80  


Ser Leu Ala Val Ala Asp Leu Leu Ala Gly Leu Gly Leu Val Leu His 
                85                  90                  95      


Phe Ala Ala Val Phe Cys Ile Gly Ser Ala Glu Met Ser Leu Val Leu 
            100                 105                 110         


Val Gly Val Leu Ala Met Ala Phe Thr Ala Ser Ile Gly Ser Leu Leu 
        115                 120                 125             


Ala Ile Thr Val Asp Arg Tyr Leu Ser Leu Tyr Asn Ala Leu Thr Tyr 
    130                 135                 140                 


Tyr Ser Glu Thr Thr Val Thr Arg Thr Tyr Val Met Leu Ala Leu Val 
145                 150                 155                 160 


Trp Gly Gly Ala Leu Gly Leu Gly Leu Leu Pro Val Leu Ala Trp Asn 
                165                 170                 175     


Cys Leu Asp Gly Leu Thr Thr Cys Gly Val Val Tyr Pro Leu Ser Lys 
            180                 185                 190         


Asn His Leu Val Val Leu Ala Ile Ala Phe Phe Met Val Phe Gly Ile 
        195                 200                 205             


Met Leu Gln Leu Tyr Ala Gln Ile Cys Arg Ile Val Cys Arg His Ala 
    210                 215                 220                 


Gln Gln Ile Ala Leu Gln Arg His Leu Leu Pro Ala Ser His Tyr Val 
225                 230                 235                 240 


Ala Thr Arg Lys Gly Ile Ala Thr Leu Ala Val Val Leu Gly Ala Phe 
                245                 250                 255     


Ala Ala Cys Trp Leu Pro Phe Thr Val Tyr Cys Leu Leu Gly Asp Ala 
            260                 265                 270         


His Ser Pro Pro Leu Tyr Thr Tyr Leu Thr Leu Leu Pro Ala Thr Tyr 
        275                 280                 285             


Asn Ser Met Ile Asn Pro Ile Ile Tyr Ala Phe Arg Asn Gln Asp Val 
    290                 295                 300                 


Gln Lys Val Leu Trp Ala Val Cys Cys Cys Cys Ser Ser Ser Lys Ile 
305                 310                 315                 320 


Pro Phe Arg Ser Arg Ser Pro Ser Asp Val 
                325                 330 


<210>  6
<211>  2180
<212>  DNA
<213>  Homo sapiens

<400>  6
accccgcgcg gttccacgcc cctggccgcg gcccgggcgc tgcgctgctc gacgcggcgg       60

gcggcgggcg gggaccgggg gcgggggcgg cggcggcggc cgggagagcg gaggaggcgg      120

agcagggagc cgggagcggg ctggcccgcg ctcctcctgc tggctgggga ttttccagcc      180

tgggcgctga cgccgcggac ctccctgcga ccgtcgcgga ccatgggcga caaagggacc      240

cgagtgttca agaaggccag tccaaatgga aagctcaccg tctacctggg aaagcgggac      300

tttgtggacc acatcgacct cgtggaccct gtggatggtg tggtcctggt ggatcctgag      360

tatctcaaag agcggagagt ctatgtgacg ctgacctgcg ccttccgcta tggccgggag      420

gacctggatg tcctgggcct gacctttcgc aaggacctgt ttgtggccaa cgtacagtcg      480

ttcccaccgg cccccgagga caagaagccc ctgacgcggc tgcaggaacg cctcatcaag      540

aagctgggcg agcacgctta ccctttcacc tttgagatcc ctccaaacct tccatgttct      600

gtgacactgc agccggggcc cgaagacacg gggaaggctt gcggtgtgga ctatgaagtc      660

aaagccttct gcgcggagaa tttggaggag aagatccaca agcggaattc tgtgcgtctg      720

gtcatccgga aggttcagta tgccccagag aggcctggcc cccagcccac agccgagacc      780

accaggcagt tcctcatgtc ggacaagccc ttgcacctag aagcctctct ggataaggag      840

atctattacc atggagaacc catcagcgtc aacgtccacg tcaccaacaa caccaacaag      900

acggtgaaga agatcaagat ctcagtgcgc cagtatgcag acatctgcct tttcaacaca      960

gctcagtaca agtgccctgt tgccatggaa gaggctgatg acactgtggc acccagctcg     1020

acgttctgca aggtctacac actgaccccc ttcctagcca ataaccgaga gaagcggggc     1080

ctcgccttgg acgggaagct caagcacgaa gacacgaact tggcctctag caccctgttg     1140

agggaaggtg ccaaccgtga gatcctgggg atcattgttt cctacaaagt gaaagtgaag     1200

ctggtggtgt ctcggggcgg cgacgtggcc gtggaactgc ccttcaccct aatgcacccc     1260

aagcccaaag aggaaccccc gcatcgggaa gttccagaga acgagacgcc agtagatacc     1320

aatctcatag aacttgacac aaatgatgac gacattgtat ttgaggactt tgctcgccag     1380

agactgaaag gcatgaagga tgacaaggag gaagaggagg atggtaccgg ctctccacag     1440

ctcaacaaca gatagacggg ccggccctgc ctccacgtgg ctccggctcc actctcgtgc     1500

actcggatgc ttactcgtct tcttcctgtt ctggtttctt ttcccctttg ttcttccagt     1560

ttctaccagg gggccccgtg ggcttccaga tcacggtgat gaacctctgg cctcaggatt     1620

ggccccacat caccacgcca acaggaccac agcgcactgg ctccacccca tctctgccat     1680

ctccactccc ctccttttca tgctgtctcc cagaaaagct gccagggctc tggccttgga     1740

attggacttg agatggggag cagacagggg aggatgggga atgtgggaca cggtgtggtg     1800

ggcatgaggg cttggagggg tggggatgag ggctcaagac acgagagaag atgtccacgg     1860

tcccaggtgg ttaacaaagt tctggcagct aaaagatgac cgcgttgaag gccacctcct     1920

tctggctggg aggggcagaa ctgtggacag attctcaatg cctttttgaa gttctgaccc     1980

accaaagacc ttctgccttc accctcctcc ccacctgatg tccctctgtg tctgatagtg     2040

atgttggtga aagttcgtag accccaggag tagagaaaag caactggact gactttctta     2100

ccagcagtta cctagactga ggcaagctgt gtggactcac ccaagtatat ttcagtactg     2160

tcaggctgtg acatcttagc                                                 2180


<210>  7
<211>  410
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Gly Asp Lys Gly Thr Arg Val Phe Lys Lys Ala Ser Pro Asn Gly 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Ile Asp 
            20                  25                  30          


Leu Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Glu Tyr Leu 
        35                  40                  45              


Lys Glu Arg Arg Val Tyr Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Thr Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Val Ala Asn Val Gln Ser Phe Pro Pro Ala Pro Glu Asp Lys Lys Pro 
                85                  90                  95      


Leu Thr Arg Leu Gln Glu Arg Leu Ile Lys Lys Leu Gly Glu His Ala 
            100                 105                 110         


Tyr Pro Phe Thr Phe Glu Ile Pro Pro Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Tyr 
    130                 135                 140                 


Glu Val Lys Ala Phe Cys Ala Glu Asn Leu Glu Glu Lys Ile His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Tyr Ala Pro Glu 
                165                 170                 175     


Arg Pro Gly Pro Gln Pro Thr Ala Glu Thr Thr Arg Gln Phe Leu Met 
            180                 185                 190         


Ser Asp Lys Pro Leu His Leu Glu Ala Ser Leu Asp Lys Glu Ile Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Ile Ser Val Asn Val His Val Thr Asn Asn Thr 
    210                 215                 220                 


Asn Lys Thr Val Lys Lys Ile Lys Ile Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Asn Thr Ala Gln Tyr Lys Cys Pro Val Ala Met Glu 
                245                 250                 255     


Glu Ala Asp Asp Thr Val Ala Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Leu Thr Pro Phe Leu Ala Asn Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Leu Leu Arg Glu Gly Ala Asn Arg Glu Ile Leu Gly Ile Ile Val Ser 
305                 310                 315                 320 


Tyr Lys Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ala 
                325                 330                 335     


Val Glu Leu Pro Phe Thr Leu Met His Pro Lys Pro Lys Glu Glu Pro 
            340                 345                 350         


Pro His Arg Glu Val Pro Glu Asn Glu Thr Pro Val Asp Thr Asn Leu 
        355                 360                 365             


Ile Glu Leu Asp Thr Asn Asp Asp Asp Ile Val Phe Glu Asp Phe Ala 
    370                 375                 380                 


Arg Gln Arg Leu Lys Gly Met Lys Asp Asp Lys Glu Glu Glu Glu Asp 
385                 390                 395                 400 


Gly Thr Gly Ser Pro Gln Leu Asn Asn Arg 
                405                 410 


<210>  8
<211>  1936
<212>  DNA
<213>  Homo sapiens

<400>  8
ccccgcgtgt ctgctaggag agggcgggca gcgccgcggc gcgcgcgatc cggctgacgc       60

atctggcccc ggttccccaa gaccagagcg gggccgggag ggagggggaa gaggcgagag      120

cgcggagggc gcgcgtgcgc attggcgcgg ggaggagcag ggatcttggc agcgggcgag      180

gaggctgcga gcgagccgcg aaccgagcgg gcggcgggcg cgcgcaccat gggggagaaa      240

cccgggacca gggtcttcaa gaagtcgagc cctaactgca agctcaccgt gtacttgggc      300

aagcgggact tcgtagatca cctggacaaa gtggaccctg tagatggcgt ggtgcttgtg      360

gaccctgact acctgaagga ccgcaaagtg tttgtgaccc tcacctgcgc cttccgctat      420

ggccgtgaag acctggatgt gctgggcttg tccttccgca aagacctgtt catcgccacc      480

taccaggcct tccccccggt gcccaaccca ccccggcccc ccacccgcct gcaggaccgg      540

ctgctgagga agctgggcca gcatgcccac cccttcttct tcaccatacc ccagaatctt      600

ccatgctccg tcacactgca gccaggccca gaggatacag gaaaggcctg cggcgtagac      660

tttgagattc gagccttctg tgctaaatca ctagaagaga aaagccacaa aaggaactct      720

gtgcggctgg tgatccgaaa ggtgcagttc gccccggaga aacccggccc ccagccttca      780

gccgaaacca cacgccactt cctcatgtct gaccggtccc tgcacctcga ggcttccctg      840

gacaaggagc tgtactacca tggggagccc ctcaatgtaa atgtccacgt caccaacaac      900

tccaccaaga ccgtcaagaa gatcaaagtc tctgtgagac agtacgccga catctgcctc      960

ttcagcaccg cccagtacaa gtgtcctgtg gctcaactcg aacaagatga ccaggtatct     1020

cccagctcca cattctgtaa ggtgtacacc ataaccccac tgctcagcga caaccgggag     1080

aagcggggtc tcgccctgga tgggaaactc aagcacgagg acaccaacct ggcttccagc     1140

accatcgtga aggagggtgc caacaaggag gtgctgggaa tcctggtgtc ctacagggtc     1200

aaggtgaagc tggtggtgtc tcgaggcggg gatgtctctg tggagctgcc ttttgttctt     1260

atgcacccca agccccacga ccacatcccc ctccccagac cccagtcagc cgctccggag     1320

acagatgtcc ctgtggacac caacctcatt gaatttgata ccaactatgc cacagatgat     1380

gacattgtgt ttgaggactt tgcccggctt cggctgaagg ggatgaagga tgacgactat     1440

gatgatcaac tctgctagga agcggggtgg gaagaaggga ggggatgggg ttgggagagg     1500

tgagggcagg attaagatcc ccactgtcaa tgggggattg tcccagcccc tcttcccttc     1560

ccctcacctg gaagcttctt caaccaatcc cttcacactc tctcccccat ccccccaaga     1620

tacacactgg accctctctt gctgaatgtg ggcattaatt ttttgactgc agctctgctt     1680

ctccagcccc gccgtgggtg gcaagctgtg ttcataccta aattttctgg aaggggacag     1740

tgaaaagagg agtgacagga gggaaagggg gagacaaaac tcctactctc aacctcacac     1800

caacacctcc cattatcact ctctctgccc ccattccttc aagaggagac cctttgggga     1860

caaggccgtt tctttgtttc tgagcataaa gaagaaaata aatcttttac taagcatgaa     1920

aaaaaaaaaa aaaaaa                                                     1936


<210>  9
<211>  409
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn 
1               5                   10                  15      


Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu 
            20                  25                  30          


Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr 
        35                  40                  45              


Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr 
    50                  55                  60                  


Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu 
65                  70                  75                  80  


Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg 
                85                  90                  95      


Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His 
            100                 105                 110         


Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val 
        115                 120                 125             


Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp 
    130                 135                 140                 


Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His 
145                 150                 155                 160 


Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro 
                165                 170                 175     


Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu 
            180                 185                 190         


Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu 
        195                 200                 205             


Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn 
    210                 215                 220                 


Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala 
225                 230                 235                 240 


Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln 
                245                 250                 255     


Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val 
            260                 265                 270         


Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu 
        275                 280                 285             


Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser 
    290                 295                 300                 


Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val 
305                 310                 315                 320 


Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val 
                325                 330                 335     


Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His 
            340                 345                 350         


Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro 
        355                 360                 365             


Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp 
    370                 375                 380                 


Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys 
385                 390                 395                 400 


Asp Asp Asp Tyr Asp Asp Gln Leu Cys 
                405                 


<210>  10
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Non-silencing siRNA

<400>  10
aauucuccga acgugucacg u                                                 21


