                         SEQUENCE LISTING

<110>  THE REGENTS OF THE UNIVERSITY OF COLORADO, a Body Corporate
       Hodges, Robert S.
       Jiang, Ziqing
 
<120>  Antimicrobial peptides and Methods of Use

<130>  68-09 WO

<140>  unassigned
<141>  2010-06-03

<150>  US 61/217,915
<151>  2009-06-05

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  1

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  2
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  2

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Leu Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  3
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  3

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  4
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  4

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Ala Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  5
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  5

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Ser Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  6
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  6

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  7
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  L at losition 13 is D-Leu.

<400>  7

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Leu Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  8
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  V at position 13 is D-Val.

<400>  8

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  9
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  A at position 13 is D-Ala.

<400>  9

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Ala Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  10
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  S at position 13 is D-Ser.

<400>  10

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Ser Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  11
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  K at position 13 is D-Lys.

<400>  11

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  12
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  12

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Gly Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  13
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  13

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Leu Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  14
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  14

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ala Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  15
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  15

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  16
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  16

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Val Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  17
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  17

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Lys Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  18
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  L at position 11 is D-Leu.

<400>  18

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Leu Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  19
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  A at position 11 is D-Ala.

<400>  19

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ala Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  20
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  S at position 11 is D-Ser.

<400>  20

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  21
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  V at position 11 is D-Val.

<400>  21

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Val Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  22
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  K at position 11 is D-Lys.

<400>  22

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Lys Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  23
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  23

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Gly Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  24
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  All amino acids are in D-conformation.

<400>  24

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  25
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  All amino acid except A at position 13 is in D-conformation.

<400>  25

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Ala Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  26
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  26

Glu Leu Glu Lys Gly Gly Leu Glu Gly Glu Lys Gly Gly Lys Glu Leu 
1               5                   10                  15      


Glu Lys 
        


<210>  27
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  27

Lys Trp Lys Ser Phe Leu Lys Thr Lys Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  28
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  28

Lys Trp Lys Ser Lys Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  29
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  A at position 9 is D-Ala.

<400>  29

Lys Trp Lys Ser Phe Leu Lys Thr Ala Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  30
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  A at position 5 is D-Ala.

<400>  30

Lys Trp Lys Ser Ala Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  31
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  31

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Arg Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  32
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  A at positions 6 and 21 is D-Ala.

<400>  32

Lys Trp Lys Ser Phe Ala Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Ala Lys Ala Ile Ser Ser 
            20                  25      


<210>  33
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  33

Lys Trp Lys Ser Phe Lys Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Lys Lys Ala Ile Ser Ser 
            20                  25      


<210>  34
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  34

Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val Leu 
1               5                   10                  15      


His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25  


<210>  35
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  35

Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val Leu His 
1               5                   10                  15      


Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  


<210>  36
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  36

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile 
            20                  


<210>  37
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  37

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala 
            20              


<210>  38
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  38

Lys Ile Lys Ser Ala Leu Lys Thr Leu Lys Ser Phe Lys Lys Thr Ala 
1               5                   10                  15      


Ala His Thr Leu Phe Lys Val Trp Ser Ser 
            20                  25      


<210>  39
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  39

Ser Trp Ser Lys Phe Leu Lys Lys Phe Thr Lys Ala Lys Ser His Val 
1               5                   10                  15      


Leu Thr Thr Ala Leu Ser Ala Ile Lys Lys 
            20                  25      


<210>  40
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(13)

<220>
<221>  MISC_FEATURE
<222>  (11)..(13)
<223>  X at positions 11 and 13 is Gly, L- or D-Leu, Val, Ser, Ala, Lys.

<400>  40

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Xaa Ala Xaa Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  41
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  41

Lys His Ala Val Ile Lys Trp Ser Ile Lys Ser Ser Val Lys Phe Lys 
1               5                   10                  15      


Ile Ser Thr Ala Phe Lys Ala Thr Thr Ile 
            20                  25      


<210>  42
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  42

His Trp Ser Lys Leu Leu Lys Ser Phe Thr Lys Ala Leu Lys Lys Phe 
1               5                   10                  15      


Ala Lys Ala Ile Thr Ser Val Val Ser Thr 
            20                  25      


<210>  43
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  X at position 11 is any amino acid in L- or D-conformation.

<400>  43

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Xaa Ala Val Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  44
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  X at position 13 is any amino acid in L- or D-conformation.

<400>  44

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Xaa Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  45
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  45

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  46
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  46

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  47
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  47

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  48
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  48

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Ala Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  49
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  49

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  50
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct: peptide

<400>  50

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  51
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct: peptide

<400>  51

Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Leu Lys Thr Val Leu 
1               5                   10                  15      


His Thr Ala Leu Lys Ala 
            20          


<210>  52
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct:  peptide


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  K at position 11 is D-lysine.

<400>  52

Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Leu Lys Thr Val Leu 
1               5                   10                  15      


His Thr Ala Leu Lys Ala 
            20          


<210>  53
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D2

<400>  53

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  54
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:   antimicrobial peptide D3

<400>  54

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  55
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D4

<400>  55

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Val 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  56
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D5

<400>  56

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  57
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: antimicrobial peptide D6

<400>  57

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Val Ile Ser Ser 
            20                  25      


<210>  58
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D7

<400>  58

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Val Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  59
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D8

<400>  59

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Val Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Val Ile Ser Ser 
            20                  25      


<210>  60
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D9

<400>  60

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Leu Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  61
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  antimicrobial peptide D10

<400>  61

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Ala Lys Lys Thr Lys 
1               5                   10                  15      


Leu His Thr Leu Leu Lys Leu Ile Ser Ser 
            20                  25      


<210>  62
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  At positions 12, 20 and 23 Xaa is Leucine, Valine or Alanine and 
       A; and at positions 13 and 16, Xaa can be Lysine, Arginine, 
       Histidine, Ornithine, diaminobutyric acid or diaminopropionic 
       acid.

<400>  62

Lys Trp Lys Ser Phe Leu Lys Thr Phe Lys Ser Xaa Xaa Lys Thr Xaa 
1               5                   10                  15      


Leu His Thr Xaa Leu Lys Xaa Ile Ser Ser 
            20                  25      


<210>  63
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D11

<400>  63

Lys Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Val 
1               5                   10                  15      


Leu Lys Thr Ala Leu Lys Ala Ile Ser Lys 
            20                  25      


<210>  64
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D12

<400>  64

Lys Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Leu Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Leu Ile Ser Lys 
            20                  25      


<210>  65
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D13

<400>  65

Lys Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Leu Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Lys 
            20                  25      


<210>  66
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D14

<400>  66

Lys Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Lys 
            20                  25      


<210>  67
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D15

<400>  67

Lys Leu Lys Ser Leu Leu Lys Thr Leu Ser Lys Ala Lys Lys Lys Leu 
1               5                   10                  15      


Leu Lys Thr Ala Leu Lys Ala Leu Ser Lys 
            20                  25      


<210>  68
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D16

<400>  68

Lys Leu Lys Ser Leu Leu Lys Thr Leu Ser Lys Ala Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Leu Ser Lys 
            20                  25      


<210>  69
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D17

<400>  69

Ser Trp Ser Ser Phe Leu Ser Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Ser Ala Ile Ser Ser 
            20                  25      


<210>  70
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D18

<400>  70

Ser Trp Ser Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Ser Ala Ile Ser Ser 
            20                  25      


<210>  71
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D19

<400>  71

Ser Trp Ser Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  72
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D20

<400>  72

Ser Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Ser 
            20                  25      


<210>  73
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D21

<400>  73

Ser Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Lys 
            20                  25      


<210>  74
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D22

<400>  74

Lys Trp Lys Ser Phe Leu Lys Thr Phe Ser Lys Ala Lys Lys Lys Ala 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Ile Ser Lys 
            20                  25      


<210>  75
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D23

<400>  75

Lys Leu Lys Ser Leu Leu Lys Thr Leu Ser Lys Leu Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Ala Leu Ser Lys 
            20                  25      


<210>  76
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  D24

<400>  76

Lys Leu Lys Ser Leu Leu Lys Thr Leu Ser Lys Leu Lys Lys Lys Lys 
1               5                   10                  15      


Leu Lys Thr Leu Leu Lys Leu Leu Ser Lys 
            20                  25      


<210>  77
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct:  consensus sequence for antimicrobial 
       peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  X is a large hydrophobic amino acid, W or L

<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  X is a large hydrophobic amino acid, F,  L or I

<220>
<221>  MISC_FEATURE
<222>  (12)..(26)
<223>  At positions 12,  20, 23 and 25,  X is a A or  L or I or V

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X is a K or  L

<400>  77

Lys Xaa Lys Ser Xaa Leu Lys Thr Xaa Ser Lys Xaa Lys Lys Lys Xaa 
1               5                   10                  15      


Leu Lys Thr Xaa Leu Lys Xaa Xaa Ser Lys 
            20                  25      


<210>  78
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide:  alternate consensus sequence of antimicrobial
       peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  At positions 1, 3, 7, 22, and 26, X is K or S

<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  At positions 12 ans 23,  X is or L or A

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X is A, V, I, L or K

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  X is I or L or A or V

<400>  78

Xaa Trp Xaa Ser Phe Leu Xaa Thr Phe Ser Lys Xaa Lys Lys Lys Xaa 
1               5                   10                  15      


Leu Lys Thr Leu Leu Xaa Xaa Xaa Ser Xaa 
            20                  25      


