                         SEQUENCE LISTING

<110>  University of Oulu
 
<120>  Process for producing natively folded disulfide bond containing 
       proteins in a prokaryotic host

<130>  BP202624

<160>  11    

<170>  PatentIn version 3.3

<210>  1
<211>  563
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Arg Leu Arg Thr Ala Ile Ala Thr Leu Cys Leu Thr Ala Phe Thr 
1               5                   10                  15      


Ser Ala Thr Ser Asn Asn Ser Tyr Ile Ala Thr Asp Gln Thr Gln Asn 
            20                  25                  30          


Ala Phe Asn Asp Thr His Phe Cys Lys Val Asp Arg Asn Asp His Val 
        35                  40                  45              


Ser Pro Ser Cys Asn Val Thr Phe Asn Glu Leu Asn Ala Ile Asn Glu 
    50                  55                  60                  


Asn Ile Arg Asp Asp Leu Ser Ala Leu Leu Lys Ser Asp Phe Phe Lys 
65                  70                  75                  80  


Tyr Phe Arg Leu Asp Leu Tyr Lys Gln Cys Ser Phe Trp Asp Ala Asn 
                85                  90                  95      


Asp Gly Leu Cys Leu Asn Arg Ala Cys Ser Val Asp Val Val Glu Asp 
            100                 105                 110         


Trp Asp Thr Leu Pro Glu Tyr Trp Gln Pro Glu Ile Leu Gly Ser Phe 
        115                 120                 125             


Asn Asn Asp Thr Met Lys Glu Ala Asp Asp Ser Asp Asp Glu Cys Lys 
    130                 135                 140                 


Phe Leu Asp Gln Leu Cys Gln Thr Ser Lys Lys Pro Val Asp Ile Glu 
145                 150                 155                 160 


Asp Thr Ile Asn Tyr Cys Asp Val Asn Asp Phe Asn Gly Lys Asn Ala 
                165                 170                 175     


Val Leu Ile Asp Leu Thr Ala Asn Pro Glu Arg Phe Thr Gly Tyr Gly 
            180                 185                 190         


Gly Lys Gln Ala Gly Gln Ile Trp Ser Thr Ile Tyr Gln Asp Asn Cys 
        195                 200                 205             


Phe Thr Ile Gly Glu Thr Gly Glu Ser Leu Ala Lys Asp Ala Phe Tyr 
    210                 215                 220                 


Arg Leu Val Ser Gly Phe His Ala Ser Ile Gly Thr His Leu Ser Lys 
225                 230                 235                 240 


Glu Tyr Leu Asn Thr Lys Thr Gly Lys Trp Glu Pro Asn Leu Asp Leu 
                245                 250                 255     


Phe Met Ala Arg Ile Gly Asn Phe Pro Asp Arg Val Thr Asn Met Tyr 
            260                 265                 270         


Phe Asn Tyr Ala Val Val Ala Lys Ala Leu Trp Lys Ile Gln Pro Tyr 
        275                 280                 285             


Leu Pro Glu Phe Ser Phe Cys Asp Leu Val Asn Lys Glu Ile Lys Asn 
    290                 295                 300                 


Lys Met Asp Asn Val Ile Ser Gln Leu Asp Thr Lys Ile Phe Asn Glu 
305                 310                 315                 320 


Asp Leu Val Phe Ala Asn Asp Leu Ser Leu Thr Leu Lys Asp Glu Phe 
                325                 330                 335     


Arg Ser Arg Phe Lys Asn Val Thr Lys Ile Met Asp Cys Val Gln Cys 
            340                 345                 350         


Asp Arg Cys Arg Leu Trp Gly Lys Ile Gln Thr Thr Gly Tyr Ala Thr 
        355                 360                 365             


Ala Leu Lys Ile Leu Phe Glu Ile Asn Asp Ala Asp Glu Phe Thr Lys 
    370                 375                 380                 


Gln His Ile Val Gly Lys Leu Thr Lys Tyr Glu Leu Ile Ala Leu Leu 
385                 390                 395                 400 


Gln Thr Phe Gly Arg Leu Ser Glu Ser Ile Glu Ser Val Asn Met Phe 
                405                 410                 415     


Glu Lys Met Tyr Gly Lys Arg Leu Asn Gly Ser Glu Asn Arg Leu Ser 
            420                 425                 430         


Ser Phe Phe Gln Asn Asn Phe Phe Asn Ile Leu Lys Glu Ala Gly Lys 
        435                 440                 445             


Ser Ile Arg Tyr Thr Ile Glu Asn Ile Asn Ser Thr Lys Glu Gly Lys 
    450                 455                 460                 


Lys Lys Thr Asn Asn Ser Gln Ser His Val Phe Asp Asp Leu Lys Met 
465                 470                 475                 480 


Pro Lys Ala Glu Ile Val Pro Arg Pro Ser Asn Gly Thr Val Asn Lys 
                485                 490                 495     


Trp Lys Lys Ala Trp Asn Thr Glu Val Asn Asn Val Leu Glu Ala Phe 
            500                 505                 510         


Arg Phe Ile Tyr Arg Ser Tyr Leu Asp Leu Pro Arg Asn Ile Trp Glu 
        515                 520                 525             


Leu Ser Leu Met Lys Val Tyr Lys Phe Trp Asn Lys Phe Ile Gly Val 
    530                 535                 540                 


Ala Asp Tyr Val Ser Glu Glu Thr Arg Glu Pro Ile Ser Tyr Lys Leu 
545                 550                 555                 560 


Asp Ile Gln 
            


<210>  2
<211>  468
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Arg Gly Trp Gly Phe Leu Phe Gly Leu Leu Gly Ala Val Trp 
1               5                   10                  15      


Leu Leu Ser Ser Gly His Gly Glu Glu Gln Pro Pro Glu Thr Ala Ala 
            20                  25                  30          


Gln Arg Cys Phe Cys Gln Val Ser Gly Tyr Leu Asp Asp Cys Thr Cys 
        35                  40                  45              


Asp Val Glu Thr Ile Asp Arg Phe Asn Asn Tyr Arg Leu Phe Pro Arg 
    50                  55                  60                  


Leu Gln Lys Leu Leu Glu Ser Asp Tyr Phe Arg Tyr Tyr Lys Val Asn 
65                  70                  75                  80  


Leu Lys Arg Pro Cys Pro Phe Trp Asn Asp Ile Ser Gln Cys Gly Arg 
                85                  90                  95      


Arg Asp Cys Ala Val Lys Pro Cys Gln Ser Asp Glu Val Pro Asp Gly 
            100                 105                 110         


Ile Lys Ser Ala Ser Tyr Lys Tyr Ser Glu Glu Ala Asn Asn Leu Ile 
        115                 120                 125             


Glu Glu Cys Glu Gln Ala Glu Arg Leu Gly Ala Val Asp Glu Ser Leu 
    130                 135                 140                 


Ser Glu Glu Thr Gln Lys Ala Val Leu Gln Trp Thr Lys His Asp Asp 
145                 150                 155                 160 


Ser Ser Asp Asn Phe Cys Glu Ala Asp Asp Ile Gln Ser Pro Glu Ala 
                165                 170                 175     


Glu Tyr Val Asp Leu Leu Leu Asn Pro Glu Arg Tyr Thr Gly Tyr Lys 
            180                 185                 190         


Gly Pro Asp Ala Trp Lys Ile Trp Asn Val Ile Tyr Glu Glu Asn Cys 
        195                 200                 205             


Phe Lys Pro Gln Thr Ile Lys Arg Pro Leu Asn Pro Leu Ala Ser Gly 
    210                 215                 220                 


Gln Gly Thr Ser Glu Glu Asn Thr Phe Tyr Ser Trp Leu Glu Gly Leu 
225                 230                 235                 240 


Cys Val Glu Lys Arg Ala Phe Tyr Arg Leu Ile Ser Gly Leu His Ala 
                245                 250                 255     


Ser Ile Asn Val His Leu Ser Ala Arg Tyr Leu Leu Gln Glu Thr Trp 
            260                 265                 270         


Leu Glu Lys Lys Trp Gly His Asn Ile Thr Glu Phe Gln Gln Arg Phe 
        275                 280                 285             


Asp Gly Ile Leu Thr Glu Gly Glu Gly Pro Arg Arg Leu Lys Asn Leu 
    290                 295                 300                 


Tyr Phe Leu Tyr Leu Ile Glu Leu Arg Ala Leu Ser Lys Val Leu Pro 
305                 310                 315                 320 


Phe Phe Glu Arg Pro Asp Phe Gln Leu Phe Thr Gly Asn Lys Ile Gln 
                325                 330                 335     


Asp Glu Glu Asn Lys Met Leu Leu Leu Glu Ile Leu His Glu Ile Lys 
            340                 345                 350         


Ser Phe Pro Leu His Phe Asp Glu Asn Ser Phe Phe Ala Gly Asp Lys 
        355                 360                 365             


Lys Glu Ala His Lys Leu Lys Glu Asp Phe Arg Leu His Phe Arg Asn 
    370                 375                 380                 


Ile Ser Arg Ile Met Asp Cys Val Gly Cys Phe Lys Cys Arg Leu Trp 
385                 390                 395                 400 


Gly Lys Leu Gln Thr Gln Gly Leu Gly Thr Ala Leu Lys Ile Leu Phe 
                405                 410                 415     


Ser Glu Lys Leu Ile Ala Asn Met Pro Glu Ser Gly Pro Ser Tyr Glu 
            420                 425                 430         


Phe His Leu Thr Arg Gln Glu Ile Val Ser Leu Phe Asn Ala Phe Gly 
        435                 440                 445             


Arg Ile Ser Thr Ser Val Lys Glu Leu Glu Asn Phe Arg Asn Leu Leu 
    450                 455                 460                 


Gln Asn Ile His 
465             


<210>  3
<211>  467
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Gln Gly Val Arg Arg Ala Gly Ala Gly Gln Gly Val Ala Ala 
1               5                   10                  15      


Ala Val Gln Leu Leu Val Thr Leu Ser Phe Leu Arg Ser Val Val Glu 
            20                  25                  30          


Ala Gln Val Thr Gly Val Leu Asp Asp Cys Leu Cys Asp Ile Asp Ser 
        35                  40                  45              


Ile Asp Asn Phe Asn Thr Tyr Lys Ile Phe Pro Lys Ile Lys Lys Leu 
    50                  55                  60                  


Gln Glu Arg Asp Tyr Phe Arg Tyr Tyr Lys Val Asn Leu Lys Arg Pro 
65                  70                  75                  80  


Cys Pro Phe Trp Ala Glu Asp Gly His Cys Ser Ile Lys Asp Cys His 
                85                  90                  95      


Val Glu Pro Cys Pro Glu Ser Lys Ile Pro Val Gly Ile Lys Ala Gly 
            100                 105                 110         


His Ser Asn Lys Tyr Leu Lys Met Ala Asn Asn Thr Lys Glu Leu Glu 
        115                 120                 125             


Asp Cys Glu Gln Ala Asn Lys Leu Gly Ala Ile Asn Ser Thr Leu Ser 
    130                 135                 140                 


Asn Gln Ser Lys Glu Ala Phe Ile Asp Trp Ala Arg Tyr Asp Asp Ser 
145                 150                 155                 160 


Arg Asp His Phe Cys Glu Leu Asp Asp Glu Arg Ser Pro Ala Ala Gln 
                165                 170                 175     


Tyr Val Asp Leu Leu Leu Asn Pro Glu Arg Tyr Thr Gly Tyr Lys Gly 
            180                 185                 190         


Thr Ser Ala Trp Arg Val Trp Asn Ser Ile Tyr Glu Glu Asn Cys Phe 
        195                 200                 205             


Lys Pro Arg Ser Val Tyr Arg Pro Leu Asn Pro Leu Ala Pro Ser Arg 
    210                 215                 220                 


Gly Glu Asp Asp Gly Glu Ser Phe Tyr Thr Trp Leu Glu Gly Leu Cys 
225                 230                 235                 240 


Leu Glu Lys Arg Val Phe Tyr Lys Leu Ile Ser Gly Leu His Ala Ser 
                245                 250                 255     


Ile Asn Leu His Leu Cys Ala Asn Tyr Leu Leu Glu Glu Thr Trp Gly 
            260                 265                 270         


Lys Pro Ser Trp Gly Pro Asn Ile Lys Glu Phe Lys His Arg Phe Asp 
        275                 280                 285             


Pro Val Glu Thr Lys Gly Glu Gly Pro Arg Arg Leu Lys Asn Leu Tyr 
    290                 295                 300                 


Phe Leu Tyr Leu Ile Glu Leu Arg Ala Leu Ser Lys Val Ala Pro Tyr 
305                 310                 315                 320 


Phe Glu Arg Ser Ile Val Asp Leu Tyr Thr Gly Asn Ala Glu Glu Asp 
                325                 330                 335     


Ala Asp Thr Lys Thr Leu Leu Leu Asn Ile Phe Gln Asp Thr Lys Ser 
            340                 345                 350         


Phe Pro Met His Phe Asp Glu Lys Ser Met Phe Ala Gly Asp Lys Lys 
        355                 360                 365             


Gly Ala Lys Ser Leu Lys Glu Glu Phe Arg Leu His Phe Lys Asn Ile 
    370                 375                 380                 


Ser Arg Ile Met Asp Cys Val Gly Cys Asp Lys Cys Arg Leu Trp Gly 
385                 390                 395                 400 


Lys Leu Gln Thr Gln Gly Leu Gly Thr Ala Leu Lys Ile Leu Phe Ser 
                405                 410                 415     


Glu Lys Glu Ile Gln Lys Leu Pro Glu Asn Ser Pro Ser Lys Gly Phe 
            420                 425                 430         


Gln Leu Thr Arg Gln Glu Ile Val Ala Leu Leu Asn Ala Phe Gly Arg 
        435                 440                 445             


Leu Ser Thr Ser Ile Arg Asp Leu Gln Asn Phe Lys Val Leu Leu Gln 
    450                 455                 460                 


His Ser Arg 
465         


<210>  4
<211>  205
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Ala Pro Gly Glu Arg Gly Arg Phe His Gly Gly Asn Leu Phe 
1               5                   10                  15      


Phe Leu Pro Gly Gly Ala Arg Ser Glu Met Met Asp Asp Leu Ala Thr 
            20                  25                  30          


Asp Ala Arg Gly Arg Gly Ala Gly Arg Arg Asp Ala Ala Ala Ser Ala 
        35                  40                  45              


Ser Thr Pro Ala Gln Ala Pro Thr Ser Asp Ser Pro Val Ala Glu Asp 
    50                  55                  60                  


Ala Ser Arg Arg Arg Pro Cys Arg Ala Cys Val Asp Phe Lys Thr Trp 
65                  70                  75                  80  


Met Arg Thr Gln Gln Lys Arg Asp Thr Lys Phe Arg Glu Asp Cys Pro 
                85                  90                  95      


Pro Asp Arg Glu Glu Leu Gly Arg His Ser Trp Ala Val Leu His Thr 
            100                 105                 110         


Leu Ala Ala Tyr Tyr Pro Asp Leu Pro Thr Pro Glu Gln Gln Gln Asp 
        115                 120                 125             


Met Ala Gln Phe Ile His Leu Phe Ser Lys Phe Tyr Pro Cys Glu Glu 
    130                 135                 140                 


Cys Ala Glu Asp Leu Arg Lys Arg Leu Cys Arg Asn His Pro Asp Thr 
145                 150                 155                 160 


Arg Thr Arg Ala Cys Phe Thr Gln Trp Leu Cys His Leu His Asn Glu 
                165                 170                 175     


Val Asn Arg Lys Leu Gly Lys Pro Asp Phe Asp Cys Ser Lys Val Asp 
            180                 185                 190         


Glu Arg Trp Arg Asp Gly Trp Lys Asp Gly Ser Cys Asp 
        195                 200                 205 


<210>  5
<211>  189
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  5

Met Lys Ala Ile Asp Lys Met Thr Asp Asn Pro Pro Gln Glu Gly Leu 
1               5                   10                  15      


Ser Gly Arg Lys Ile Ile Tyr Asp Glu Asp Gly Lys Pro Cys Arg Ser 
            20                  25                  30          


Cys Asn Thr Leu Leu Asp Phe Gln Tyr Val Thr Gly Lys Ile Ser Asn 
        35                  40                  45              


Gly Leu Lys Asn Leu Ser Ser Asn Gly Lys Leu Ala Gly Thr Gly Ala 
    50                  55                  60                  


Leu Thr Gly Glu Ala Ser Glu Leu Met Pro Gly Ser Arg Thr Tyr Arg 
65                  70                  75                  80  


Lys Val Asp Pro Pro Asp Val Glu Gln Leu Gly Arg Ser Ser Trp Thr 
                85                  90                  95      


Leu Leu His Ser Val Ala Ala Ser Tyr Pro Ala Gln Pro Thr Asp Gln 
            100                 105                 110         


Gln Lys Gly Glu Met Lys Gln Phe Leu Asn Ile Phe Ser His Ile Tyr 
        115                 120                 125             


Pro Cys Asn Trp Cys Ala Lys Asp Phe Glu Lys Tyr Ile Arg Glu Asn 
    130                 135                 140                 


Ala Pro Gln Val Glu Ser Arg Glu Glu Leu Gly Arg Trp Met Cys Glu 
145                 150                 155                 160 


Ala His Asn Lys Val Asn Lys Lys Leu Arg Lys Pro Lys Phe Asp Cys 
                165                 170                 175     


Asn Phe Trp Glu Lys Arg Trp Lys Asp Gly Trp Asp Glu 
            180                 185                 


<210>  6
<211>  196
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Lys Gln Ile Val Lys Arg Ser His Ala Ile Arg Ile Val Ala Ala 
1               5                   10                  15      


Leu Gly Ile Ile Gly Leu Trp Met Phe Phe Ser Ser Asn Glu Leu Ser 
            20                  25                  30          


Ile Ala Thr Pro Gly Leu Ile Lys Ala Lys Ser Gly Ile Asp Glu Val 
        35                  40                  45              


Gln Gly Ala Ala Ala Glu Lys Asn Asp Ala Arg Leu Lys Glu Ile Glu 
    50                  55                  60                  


Lys Gln Thr Ile Met Pro Leu Met Gly Asp Asp Lys Val Lys Lys Glu 
65                  70                  75                  80  


Val Gly Arg Ala Ser Trp Lys Tyr Phe His Thr Leu Leu Ala Arg Phe 
                85                  90                  95      


Pro Asp Glu Pro Thr Pro Glu Glu Arg Glu Lys Leu His Thr Phe Ile 
            100                 105                 110         


Gly Leu Tyr Ala Glu Leu Tyr Pro Cys Gly Glu Cys Ser Tyr His Phe 
        115                 120                 125             


Val Lys Leu Ile Glu Lys Tyr Pro Val Gln Thr Ser Ser Arg Thr Ala 
    130                 135                 140                 


Ala Ala Met Trp Gly Cys His Ile His Asn Lys Val Asn Glu Tyr Leu 
145                 150                 155                 160 


Lys Lys Asp Ile Tyr Asp Cys Ala Thr Ile Leu Glu Asp Tyr Asp Cys 
                165                 170                 175     


Gly Cys Ser Asp Ser Asp Gly Lys Arg Val Ser Leu Glu Lys Glu Ala 
            180                 185                 190         


Lys Gln His Gly 
        195     


<210>  7
<211>  747
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Arg Arg Cys Asn Ser Gly Ser Gly Pro Pro Pro Ser Leu Leu Leu 
1               5                   10                  15      


Leu Leu Leu Trp Leu Leu Ala Val Pro Gly Ala Asn Ala Ala Pro Arg 
            20                  25                  30          


Ser Ala Leu Tyr Ser Pro Ser Asp Pro Leu Thr Leu Leu Gln Ala Asp 
        35                  40                  45              


Thr Val Arg Gly Ala Val Leu Gly Ser Arg Ser Ala Trp Ala Val Glu 
    50                  55                  60                  


Phe Phe Ala Ser Trp Cys Gly His Cys Ile Ala Phe Ala Pro Thr Trp 
65                  70                  75                  80  


Lys Ala Leu Ala Glu Asp Val Lys Ala Trp Arg Pro Ala Leu Tyr Leu 
                85                  90                  95      


Ala Ala Leu Asp Cys Ala Glu Glu Thr Asn Ser Ala Val Cys Arg Asp 
            100                 105                 110         


Phe Asn Ile Pro Gly Phe Pro Thr Val Arg Phe Phe Lys Ala Phe Thr 
        115                 120                 125             


Lys Asn Gly Ser Gly Ala Val Phe Pro Val Ala Gly Ala Asp Val Gln 
    130                 135                 140                 


Thr Leu Arg Glu Arg Leu Ile Asp Ala Leu Glu Ser His His Asp Thr 
145                 150                 155                 160 


Trp Pro Pro Ala Cys Pro Pro Leu Glu Pro Ala Lys Leu Glu Glu Ile 
                165                 170                 175     


Asp Gly Phe Phe Ala Arg Asn Asn Glu Glu Tyr Leu Ala Leu Ile Phe 
            180                 185                 190         


Glu Lys Gly Gly Ser Tyr Leu Gly Arg Glu Val Ala Leu Asp Leu Ser 
        195                 200                 205             


Gln His Lys Gly Val Ala Val Arg Arg Val Leu Asn Thr Glu Ala Asn 
    210                 215                 220                 


Val Val Arg Lys Phe Gly Val Thr Asp Phe Pro Ser Cys Tyr Leu Leu 
225                 230                 235                 240 


Phe Arg Asn Gly Ser Val Ser Arg Val Pro Val Leu Met Glu Ser Arg 
                245                 250                 255     


Ser Phe Tyr Thr Ala Tyr Leu Gln Arg Leu Ser Gly Leu Thr Arg Glu 
            260                 265                 270         


Ala Ala Gln Thr Thr Val Ala Pro Thr Thr Ala Asn Lys Ile Ala Pro 
        275                 280                 285             


Thr Val Trp Lys Leu Ala Asp Arg Ser Lys Ile Tyr Met Ala Asp Leu 
    290                 295                 300                 


Glu Ser Ala Leu His Tyr Ile Leu Arg Ile Glu Val Gly Arg Phe Pro 
305                 310                 315                 320 


Val Leu Glu Gly Gln Arg Leu Val Ala Leu Lys Lys Phe Val Ala Val 
                325                 330                 335     


Leu Ala Lys Tyr Phe Pro Gly Arg Pro Leu Val Gln Asn Phe Leu His 
            340                 345                 350         


Ser Val Asn Glu Trp Leu Lys Arg Gln Lys Arg Asn Lys Ile Pro Tyr 
        355                 360                 365             


Ser Phe Phe Lys Thr Ala Leu Asp Asp Arg Lys Glu Gly Ala Val Leu 
    370                 375                 380                 


Ala Lys Lys Val Asn Trp Ile Gly Cys Gln Gly Ser Glu Pro His Phe 
385                 390                 395                 400 


Arg Gly Phe Pro Cys Ser Leu Trp Val Leu Phe His Phe Leu Thr Val 
                405                 410                 415     


Gln Ala Ala Arg Gln Asn Val Asp His Ser Gln Glu Ala Ala Lys Ala 
            420                 425                 430         


Lys Glu Val Leu Pro Ala Ile Arg Gly Tyr Val His Tyr Phe Phe Gly 
        435                 440                 445             


Cys Arg Asp Cys Ala Ser His Phe Glu Gln Met Ala Ala Ala Ser Met 
    450                 455                 460                 


His Arg Val Gly Ser Pro Asn Ala Ala Val Leu Trp Leu Trp Ser Ser 
465                 470                 475                 480 


His Asn Arg Val Asn Ala Arg Leu Ala Gly Ala Pro Ser Glu Asp Pro 
                485                 490                 495     


Gln Phe Pro Lys Val Gln Trp Pro Pro Arg Glu Leu Cys Ser Ala Cys 
            500                 505                 510         


His Asn Glu Arg Leu Asp Val Pro Val Trp Asp Val Glu Ala Thr Leu 
        515                 520                 525             


Asn Phe Leu Lys Ala His Phe Ser Pro Ser Asn Ile Ile Leu Asp Phe 
    530                 535                 540                 


Pro Ala Ala Gly Ser Ala Ala Arg Arg Asp Val Gln Asn Val Ala Ala 
545                 550                 555                 560 


Ala Pro Glu Leu Ala Met Gly Ala Leu Glu Leu Glu Ser Arg Asn Ser 
                565                 570                 575     


Thr Leu Asp Pro Gly Lys Pro Glu Met Met Lys Ser Pro Thr Asn Thr 
            580                 585                 590         


Thr Pro His Val Pro Ala Glu Gly Pro Glu Ala Ser Arg Pro Pro Lys 
        595                 600                 605             


Leu His Pro Gly Leu Arg Ala Ala Pro Gly Gln Glu Pro Pro Glu His 
    610                 615                 620                 


Met Ala Glu Leu Gln Arg Asn Glu Gln Glu Gln Pro Leu Gly Gln Trp 
625                 630                 635                 640 


His Leu Ser Lys Arg Asp Thr Gly Ala Ala Leu Leu Ala Glu Ser Arg 
                645                 650                 655     


Ala Glu Lys Asn Arg Leu Trp Gly Pro Leu Glu Val Arg Arg Val Gly 
            660                 665                 670         


Arg Ser Ser Lys Gln Leu Val Asp Ile Pro Glu Gly Gln Leu Glu Ala 
        675                 680                 685             


Arg Ala Gly Arg Gly Arg Gly Gln Trp Leu Gln Val Leu Gly Gly Gly 
    690                 695                 700                 


Phe Ser Tyr Leu Asp Ile Ser Leu Cys Val Gly Leu Tyr Ser Leu Ser 
705                 710                 715                 720 


Phe Met Gly Leu Leu Ala Met Tyr Thr Tyr Phe Gln Ala Lys Ile Arg 
                725                 730                 735     


Ala Leu Lys Gly His Ala Gly His Pro Ala Ala 
            740                 745         


<210>  8
<211>  698
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ala Ala Ala Gly Ala Ala Val Ala Arg Ser Pro Gly Ile Gly Ala 
1               5                   10                  15      


Gly Pro Ala Leu Arg Ala Arg Arg Ser Pro Pro Pro Arg Ala Ala Arg 
            20                  25                  30          


Leu Pro Arg Leu Leu Val Leu Leu Ala Ala Ala Ala Val Gly Pro Gly 
        35                  40                  45              


Ala Gly Gly Ala Ala Arg Leu Tyr Arg Ala Gly Glu Asp Ala Val Trp 
    50                  55                  60                  


Val Leu Asp Ser Gly Ser Val Arg Gly Ala Thr Ala Asn Ser Ser Ala 
65                  70                  75                  80  


Ala Trp Leu Val Gln Phe Tyr Ser Ser Trp Cys Gly His Cys Ile Gly 
                85                  90                  95      


Tyr Ala Pro Thr Trp Arg Ala Leu Ala Gly Asp Val Arg Asp Trp Ala 
            100                 105                 110         


Ser Ala Ile Arg Val Ala Ala Leu Asp Cys Met Glu Glu Lys Asn Gln 
        115                 120                 125             


Ala Val Cys His Asp Tyr Asp Ile His Phe Tyr Pro Thr Phe Arg Tyr 
    130                 135                 140                 


Phe Lys Ala Phe Thr Lys Glu Phe Thr Thr Gly Glu Asn Phe Lys Gly 
145                 150                 155                 160 


Pro Asp Arg Glu Leu Arg Thr Val Arg Gln Thr Met Ile Asp Phe Leu 
                165                 170                 175     


Gln Asn His Thr Glu Gly Ser Arg Pro Pro Ala Cys Pro Arg Leu Asp 
            180                 185                 190         


Pro Ile Gln Pro Ser Asp Val Leu Ser Leu Leu Asp Asn Arg Gly Ser 
        195                 200                 205             


His Tyr Val Ala Ile Val Phe Glu Ser Asn Ser Ser Tyr Leu Gly Arg 
    210                 215                 220                 


Glu Val Ile Leu Asp Leu Ile Pro Tyr Glu Ser Ile Val Val Thr Arg 
225                 230                 235                 240 


Ala Leu Asp Gly Asp Lys Ala Phe Leu Glu Lys Leu Gly Val Ser Ser 
                245                 250                 255     


Val Pro Ser Cys Tyr Leu Ile Tyr Pro Asn Gly Ser His Gly Leu Ile 
            260                 265                 270         


Asn Val Val Lys Pro Leu Arg Ala Phe Phe Ser Ser Tyr Leu Lys Ser 
        275                 280                 285             


Leu Pro Asp Val Arg Lys Lys Ser Leu Pro Leu Pro Glu Lys Pro His 
    290                 295                 300                 


Lys Glu Glu Asn Ser Glu Ile Val Val Trp Arg Glu Phe Asp Lys Ser 
305                 310                 315                 320 


Lys Leu Tyr Thr Val Asp Leu Glu Ser Gly Leu His Tyr Leu Leu Arg 
                325                 330                 335     


Val Glu Leu Ala Ala His Lys Ser Leu Ala Gly Ala Glu Leu Lys Thr 
            340                 345                 350         


Leu Lys Asp Phe Val Thr Val Leu Ala Lys Leu Phe Pro Gly Arg Pro 
        355                 360                 365             


Pro Val Lys Lys Leu Leu Glu Met Leu Gln Glu Trp Leu Ala Ser Leu 
    370                 375                 380                 


Pro Leu Asp Arg Ile Pro Tyr Asn Ala Val Leu Asp Leu Val Asn Asn 
385                 390                 395                 400 


Lys Met Arg Ile Ser Gly Ile Phe Leu Thr Asn His Ile Lys Trp Val 
                405                 410                 415     


Gly Cys Gln Gly Ser Arg Ser Glu Leu Arg Gly Tyr Pro Cys Ser Leu 
            420                 425                 430         


Trp Lys Leu Phe His Thr Leu Thr Val Glu Ala Ser Thr His Pro Asp 
        435                 440                 445             


Ala Leu Val Gly Thr Gly Phe Glu Asp Asp Pro Gln Ala Val Leu Gln 
    450                 455                 460                 


Thr Met Arg Arg Tyr Val His Thr Phe Phe Gly Cys Lys Glu Cys Gly 
465                 470                 475                 480 


Glu His Phe Glu Glu Met Ala Lys Glu Ser Met Asp Ser Val Lys Thr 
                485                 490                 495     


Pro Asp Gln Ala Ile Leu Trp Leu Trp Lys Lys His Asn Met Val Asn 
            500                 505                 510         


Gly Arg Leu Ala Gly His Leu Ser Glu Asp Pro Arg Phe Pro Lys Leu 
        515                 520                 525             


Gln Trp Pro Thr Pro Asp Leu Cys Pro Ala Cys His Glu Glu Ile Lys 
    530                 535                 540                 


Gly Leu Ala Ser Trp Asp Glu Gly His Val Leu Thr Phe Leu Lys Gln 
545                 550                 555                 560 


His Tyr Gly Arg Asp Asn Leu Leu Asp Thr Tyr Ser Ala Asp Gln Gly 
                565                 570                 575     


Asp Ser Ser Glu Gly Gly Thr Leu Ala Arg Gly Glu Glu Glu Glu Lys 
            580                 585                 590         


Arg Leu Thr Pro Pro Glu Val Ser His Gly Asp Arg Asp Thr Gln Ser 
        595                 600                 605             


Val Arg Pro Pro Gly Ala Leu Gly Pro Arg Pro Ala Leu Pro Glu Ser 
    610                 615                 620                 


Leu His His Ser Leu Asp Gly Lys Leu Gln Ser Leu Asp Gly Pro Gly 
625                 630                 635                 640 


Ala His Lys Glu Val Gly Gly Ala Ala Pro Phe Leu Gly Val Asp Phe 
                645                 650                 655     


Ser Ser Leu Asp Met Ser Leu Cys Val Val Leu Tyr Val Ala Ser Ser 
            660                 665                 670         


Leu Phe Leu Met Val Met Tyr Phe Phe Phe Arg Val Arg Ser Arg Arg 
        675                 680                 685             


Trp Lys Val Lys His His His Pro Ala Val 
    690                 695             


<210>  9
<211>  95
<212>  PRT
<213>  Vaccinia virus

<400>  9

Met Asn Pro Lys His Trp Gly Arg Ala Val Trp Thr Ile Ile Phe Ile 
1               5                   10                  15      


Val Leu Ser Gln Ala Gly Leu Asp Gly Asn Ile Glu Ala Cys Lys Arg 
            20                  25                  30          


Lys Leu Tyr Thr Ile Val Ser Thr Leu Pro Cys Pro Ala Cys Arg Arg 
        35                  40                  45              


His Ala Thr Ile Ala Ile Glu Asp Asn Asn Val Met Ser Ser Asp Asp 
    50                  55                  60                  


Leu Asn Tyr Ile Tyr Tyr Phe Phe Ile Arg Leu Phe Asn Asn Leu Ala 
65                  70                  75                  80  


Ser Asp Pro Lys Tyr Ala Ile Asp Val Ser Lys Val Lys Pro Leu 
                85                  90                  95  


<210>  10
<211>  236
<212>  PRT
<213>  Escherichia coli

<400>  10

Met Lys Lys Gly Phe Met Leu Phe Thr Leu Leu Ala Ala Phe Ser Gly 
1               5                   10                  15      


Phe Ala Gln Ala Asp Asp Ala Ala Ile Gln Gln Thr Leu Ala Lys Met 
            20                  25                  30          


Gly Ile Lys Ser Ser Asp Ile Gln Pro Ala Pro Val Ala Gly Met Lys 
        35                  40                  45              


Thr Val Leu Thr Asn Ser Gly Val Leu Tyr Ile Thr Asp Asp Gly Lys 
    50                  55                  60                  


His Ile Ile Gln Gly Pro Met Tyr Asp Val Ser Gly Thr Ala Pro Val 
65                  70                  75                  80  


Asn Val Thr Asn Lys Met Leu Leu Lys Gln Leu Asn Ala Leu Glu Lys 
                85                  90                  95      


Glu Met Ile Val Tyr Lys Ala Pro Gln Glu Lys His Val Ile Thr Val 
            100                 105                 110         


Phe Thr Asp Ile Thr Cys Gly Tyr Cys His Lys Leu His Glu Gln Met 
        115                 120                 125             


Ala Asp Tyr Asn Ala Leu Gly Ile Thr Val Arg Tyr Leu Ala Phe Pro 
    130                 135                 140                 


Arg Gln Gly Leu Asp Ser Asp Ala Glu Lys Glu Met Lys Ala Ile Trp 
145                 150                 155                 160 


Cys Ala Lys Asp Lys Asn Lys Ala Phe Asp Asp Val Met Ala Gly Lys 
                165                 170                 175     


Ser Val Ala Pro Ala Ser Cys Asp Val Asp Ile Ala Asp His Tyr Ala 
            180                 185                 190         


Leu Gly Val Gln Leu Gly Val Ser Gly Thr Pro Ala Val Val Leu Ser 
        195                 200                 205             


Asn Gly Thr Leu Val Pro Gly Tyr Gln Pro Pro Lys Glu Met Lys Glu 
    210                 215                 220                 


Phe Leu Asp Glu His Gln Lys Met Thr Ser Gly Lys 
225                 230                 235     


<210>  11
<211>  508
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Leu Arg Arg Ala Leu Leu Cys Leu Ala Val Ala Ala Leu Val Arg 
1               5                   10                  15      


Ala Asp Ala Pro Glu Glu Glu Asp His Val Leu Val Leu Arg Lys Ser 
            20                  25                  30          


Asn Phe Ala Glu Ala Leu Ala Ala His Lys Tyr Leu Leu Val Glu Phe 
        35                  40                  45              


Tyr Ala Pro Trp Cys Gly His Cys Lys Ala Leu Ala Pro Glu Tyr Ala 
    50                  55                  60                  


Lys Ala Ala Gly Lys Leu Lys Ala Glu Gly Ser Glu Ile Arg Leu Ala 
65                  70                  75                  80  


Lys Val Asp Ala Thr Glu Glu Ser Asp Leu Ala Gln Gln Tyr Gly Val 
                85                  90                  95      


Arg Gly Tyr Pro Thr Ile Lys Phe Phe Arg Asn Gly Asp Thr Ala Ser 
            100                 105                 110         


Pro Lys Glu Tyr Thr Ala Gly Arg Glu Ala Asp Asp Ile Val Asn Trp 
        115                 120                 125             


Leu Lys Lys Arg Thr Gly Pro Ala Ala Thr Thr Leu Pro Asp Gly Ala 
    130                 135                 140                 


Ala Ala Glu Ser Leu Val Glu Ser Ser Glu Val Ala Val Ile Gly Phe 
145                 150                 155                 160 


Phe Lys Asp Val Glu Ser Asp Ser Ala Lys Gln Phe Leu Gln Ala Ala 
                165                 170                 175     


Glu Ala Ile Asp Asp Ile Pro Phe Gly Ile Thr Ser Asn Ser Asp Val 
            180                 185                 190         


Phe Ser Lys Tyr Gln Leu Asp Lys Asp Gly Val Val Leu Phe Lys Lys 
        195                 200                 205             


Phe Asp Glu Gly Arg Asn Asn Phe Glu Gly Glu Val Thr Lys Glu Asn 
    210                 215                 220                 


Leu Leu Asp Phe Ile Lys His Asn Gln Leu Pro Leu Val Ile Glu Phe 
225                 230                 235                 240 


Thr Glu Gln Thr Ala Pro Lys Ile Phe Gly Gly Glu Ile Lys Thr His 
                245                 250                 255     


Ile Leu Leu Phe Leu Pro Lys Ser Val Ser Asp Tyr Asp Gly Lys Leu 
            260                 265                 270         


Ser Asn Phe Lys Thr Ala Ala Glu Ser Phe Lys Gly Lys Ile Leu Phe 
        275                 280                 285             


Ile Phe Ile Asp Ser Asp His Thr Asp Asn Gln Arg Ile Leu Glu Phe 
    290                 295                 300                 


Phe Gly Leu Lys Lys Glu Glu Cys Pro Ala Val Arg Leu Ile Thr Leu 
305                 310                 315                 320 


Glu Glu Glu Met Thr Lys Tyr Lys Pro Glu Ser Glu Glu Leu Thr Ala 
                325                 330                 335     


Glu Arg Ile Thr Glu Phe Cys His Arg Phe Leu Glu Gly Lys Ile Lys 
            340                 345                 350         


Pro His Leu Met Ser Gln Glu Leu Pro Glu Asp Trp Asp Lys Gln Pro 
        355                 360                 365             


Val Lys Val Leu Val Gly Lys Asn Phe Glu Asp Val Ala Phe Asp Glu 
    370                 375                 380                 


Lys Lys Asn Val Phe Val Glu Phe Tyr Ala Pro Trp Cys Gly His Cys 
385                 390                 395                 400 


Lys Gln Leu Ala Pro Ile Trp Asp Lys Leu Gly Glu Thr Tyr Lys Asp 
                405                 410                 415     


His Glu Asn Ile Val Ile Ala Lys Met Asp Ser Thr Ala Asn Glu Val 
            420                 425                 430         


Glu Ala Val Lys Val His Ser Phe Pro Thr Leu Lys Phe Phe Pro Ala 
        435                 440                 445             


Ser Ala Asp Arg Thr Val Ile Asp Tyr Asn Gly Glu Arg Thr Leu Asp 
    450                 455                 460                 


Gly Phe Lys Lys Phe Leu Glu Ser Gly Gly Gln Asp Gly Ala Gly Asp 
465                 470                 475                 480 


Asp Asp Asp Leu Glu Asp Leu Glu Glu Ala Glu Glu Pro Asp Met Glu 
                485                 490                 495     


Glu Asp Asp Asp Gln Lys Ala Val Lys Asp Glu Leu 
            500                 505             


