                         SEQUENCE LISTING

<110>  INSTITUT PASTEUR
 
<120>  Mutant CyaA polypeptides and polypeptide derivatives suitable for
        the delivery of immunogenic molecules into a cell

<130>  B08142B

<150>  EP 09155929.4
<151>  2009-03-23

<150>  US 12/409324
<151>  2009-03-23

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  1706
<212>  PRT
<213>  Bordetella pertussis

<400>  1

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Val Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ser  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly Gln  Asn Thr Val Ile Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Arg  Leu Asn Asp 
    1130                 1135                 1140             


Arg Ile  Ala Gly Asp Asp Gln  Asp Asn Glu Leu Trp  Gly His Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile Arg Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Pro 
    1205                 1210                 1215             


Gly Arg  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Arg Glu Trp Val  Arg Lys Ala Ser Ala  Leu Gly Val 
    1235                 1240                 1245             


Asp Tyr  Tyr Asp Asn Val Arg  Asn Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Gln Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Asp Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Thr Gly Ala His  Ala Gly Ile Ala Ala  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Asp Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Arg Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg Asn Ile Glu  Asn Ala Val Gly Ser  Ala Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Val Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ile  Ile His Ala Ala Asn  Gln Ala Val Asp Gln  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  2
<211>  1706
<212>  PRT
<213>  Artificial

<220>
<223>  CyaA/E570Q+K860R

<400>  2

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Ala Gly Ala Gln Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Arg Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Val Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ser  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly Gln  Asn Thr Val Ile Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Arg  Leu Asn Asp 
    1130                 1135                 1140             


Arg Ile  Ala Gly Asp Asp Gln  Asp Asn Glu Leu Trp  Gly His Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile Arg Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Pro 
    1205                 1210                 1215             


Gly Arg  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Arg Glu Trp Val  Arg Lys Ala Ser Ala  Leu Gly Val 
    1235                 1240                 1245             


Asp Tyr  Tyr Asp Asn Val Arg  Asn Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Gln Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Asp Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Thr Gly Ala His  Ala Gly Ile Ala Ala  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Asp Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Arg Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg Asn Ile Glu  Asn Ala Val Gly Ser  Ala Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Val Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ile  Ile His Ala Ala Asn  Gln Ala Val Asp Gln  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  3
<211>  1708
<212>  PRT
<213>  Artificial

<220>
<223>  CyaA/E570Q+K860R/AC-

<400>  3

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Gly Ser Ile Asp 
            180                 185                 190         


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
        195                 200                 205             


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
    210                 215                 220                 


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile 
225                 230                 235                 240 


Asp Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly 
                245                 250                 255     


Thr Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly 
            260                 265                 270         


Val Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg 
        275                 280                 285             


Ala His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln 
    290                 295                 300                 


Asn Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala 
305                 310                 315                 320 


Thr Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile 
                325                 330                 335     


Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr 
            340                 345                 350         


Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro 
        355                 360                 365             


Gly Val Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr 
    370                 375                 380                 


Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu 
385                 390                 395                 400 


Arg Gln Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser 
                405                 410                 415     


Phe Ser Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu 
            420                 425                 430         


Leu Glu Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp 
        435                 440                 445             


Ala Glu Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala 
    450                 455                 460                 


Leu Gln Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala 
465                 470                 475                 480 


Ile Ala Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro 
                485                 490                 495     


Gln Glu Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala 
            500                 505                 510         


Ser Ser Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser 
        515                 520                 525             


Arg Trp Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly 
    530                 535                 540                 


Gly Gly Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp 
545                 550                 555                 560 


Ala Pro Ala Gly Gln Lys Ala Ala Ala Gly Ala Gln Ile Ala Leu Gln 
                565                 570                 575     


Leu Thr Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu 
            580                 585                 590         


Ala Ala Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser 
        595                 600                 605             


Ala Gly Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu 
    610                 615                 620                 


Ile Tyr Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys 
625                 630                 635                 640 


Leu Ala Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu 
                645                 650                 655     


Ala Gln Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly 
            660                 665                 670         


Val Ser Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala 
        675                 680                 685             


Ala Ala Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu 
    690                 695                 700                 


Thr Gly Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile 
705                 710                 715                 720 


Glu Lys Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly 
                725                 730                 735     


Pro Gln Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu 
            740                 745                 750         


Ala Asn Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly 
        755                 760                 765             


Trp Asn Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys 
    770                 775                 780                 


Ser Ala Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys 
785                 790                 795                 800 


Ser Val Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala 
                805                 810                 815     


Gly Gln Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala 
            820                 825                 830         


Ser Arg Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala 
        835                 840                 845             


Ala Pro Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Arg Ser Glu 
    850                 855                 860                 


Phe Thr Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile 
865                 870                 875                 880 


Arg Asp Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser 
                885                 890                 895     


Gln Leu Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp 
            900                 905                 910         


Val Ile Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg 
        915                 920                 925             


Ile His Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala 
    930                 935                 940                 


Leu Gly Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe 
945                 950                 955                 960 


Asn Val Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val 
                965                 970                 975     


Ala Thr Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr 
            980                 985                 990         


Arg His Val Glu Leu Ala Arg Val  Gly Gln Val Val Glu  Val Asp Thr 
        995                 1000                 1005             


Leu Glu  His Val Gln His Ile  Ile Gly Gly Ala Gly  Asn Asp Ser 
    1010                 1015                 1020             


Ile Thr  Gly Asn Ala His Asp  Asn Phe Leu Ala Gly  Gly Ser Gly 
    1025                 1030                 1035             


Asp Asp  Arg Leu Asp Gly Gly  Ala Gly Asn Asp Thr  Leu Val Gly 
    1040                 1045                 1050             


Gly Glu  Gly Gln Asn Thr Val  Ile Gly Gly Ala Gly  Asp Asp Val 
    1055                 1060                 1065             


Phe Leu  Gln Asp Leu Gly Val  Trp Ser Asn Gln Leu  Asp Gly Gly 
    1070                 1075                 1080             


Ala Gly  Val Asp Thr Val Lys  Tyr Asn Val His Gln  Pro Ser Glu 
    1085                 1090                 1095             


Glu Arg  Leu Glu Arg Met Gly  Asp Thr Gly Ile His  Ala Asp Leu 
    1100                 1105                 1110             


Gln Lys  Gly Thr Val Glu Lys  Trp Pro Ala Leu Asn  Leu Phe Ser 
    1115                 1120                 1125             


Val Asp  His Val Lys Asn Ile  Glu Asn Leu His Gly  Ser Arg Leu 
    1130                 1135                 1140             


Asn Asp  Arg Ile Ala Gly Asp  Asp Gln Asp Asn Glu  Leu Trp Gly 
    1145                 1150                 1155             


His Asp  Gly Asn Asp Thr Ile  Arg Gly Arg Gly Gly  Asp Asp Ile 
    1160                 1165                 1170             


Leu Arg  Gly Gly Leu Gly Leu  Asp Thr Leu Tyr Gly  Glu Asp Gly 
    1175                 1180                 1185             


Asn Asp  Ile Phe Leu Gln Asp  Asp Glu Thr Val Ser  Asp Asp Ile 
    1190                 1195                 1200             


Asp Gly  Gly Ala Gly Leu Asp  Thr Val Asp Tyr Ser  Ala Met Ile 
    1205                 1210                 1215             


His Pro  Gly Arg Ile Val Ala  Pro His Glu Tyr Gly  Phe Gly Ile 
    1220                 1225                 1230             


Glu Ala  Asp Leu Ser Arg Glu  Trp Val Arg Lys Ala  Ser Ala Leu 
    1235                 1240                 1245             


Gly Val  Asp Tyr Tyr Asp Asn  Val Arg Asn Val Glu  Asn Val Ile 
    1250                 1255                 1260             


Gly Thr  Ser Met Lys Asp Val  Leu Ile Gly Asp Ala  Gln Ala Asn 
    1265                 1270                 1275             


Thr Leu  Met Gly Gln Gly Gly  Asp Asp Thr Val Arg  Gly Gly Asp 
    1280                 1285                 1290             


Gly Asp  Asp Leu Leu Phe Gly  Gly Asp Gly Asn Asp  Met Leu Tyr 
    1295                 1300                 1305             


Gly Asp  Ala Gly Asn Asp Thr  Leu Tyr Gly Gly Leu  Gly Asp Asp 
    1310                 1315                 1320             


Thr Leu  Glu Gly Gly Ala Gly  Asn Asp Trp Phe Gly  Gln Thr Gln 
    1325                 1330                 1335             


Ala Arg  Glu His Asp Val Leu  Arg Gly Gly Asp Gly  Val Asp Thr 
    1340                 1345                 1350             


Val Asp  Tyr Ser Gln Thr Gly  Ala His Ala Gly Ile  Ala Ala Gly 
    1355                 1360                 1365             


Arg Ile  Gly Leu Gly Ile Leu  Ala Asp Leu Gly Ala  Gly Arg Val 
    1370                 1375                 1380             


Asp Lys  Leu Gly Glu Ala Gly  Ser Ser Ala Tyr Asp  Thr Val Ser 
    1385                 1390                 1395             


Gly Ile  Glu Asn Val Val Gly  Thr Glu Leu Ala Asp  Arg Ile Thr 
    1400                 1405                 1410             


Gly Asp  Ala Gln Ala Asn Val  Leu Arg Gly Ala Gly  Gly Ala Asp 
    1415                 1420                 1425             


Val Leu  Ala Gly Gly Glu Gly  Asp Asp Val Leu Leu  Gly Gly Asp 
    1430                 1435                 1440             


Gly Asp  Asp Gln Leu Ser Gly  Asp Ala Gly Arg Asp  Arg Leu Tyr 
    1445                 1450                 1455             


Gly Glu  Ala Gly Asp Asp Trp  Phe Phe Gln Asp Ala  Ala Asn Ala 
    1460                 1465                 1470             


Gly Asn  Leu Leu Asp Gly Gly  Asp Gly Arg Asp Thr  Val Asp Phe 
    1475                 1480                 1485             


Ser Gly  Pro Gly Arg Gly Leu  Asp Ala Gly Ala Lys  Gly Val Phe 
    1490                 1495                 1500             


Leu Ser  Leu Gly Lys Gly Phe  Ala Ser Leu Met Asp  Glu Pro Glu 
    1505                 1510                 1515             


Thr Ser  Asn Val Leu Arg Asn  Ile Glu Asn Ala Val  Gly Ser Ala 
    1520                 1525                 1530             


Arg Asp  Asp Val Leu Ile Gly  Asp Ala Gly Ala Asn  Val Leu Asn 
    1535                 1540                 1545             


Gly Leu  Ala Gly Asn Asp Val  Leu Ser Gly Gly Ala  Gly Asp Asp 
    1550                 1555                 1560             


Val Leu  Leu Gly Asp Glu Gly  Ser Asp Leu Leu Ser  Gly Asp Ala 
    1565                 1570                 1575             


Gly Asn  Asp Asp Leu Phe Gly  Gly Gln Gly Asp Asp  Thr Tyr Leu 
    1580                 1585                 1590             


Phe Gly  Val Gly Tyr Gly His  Asp Thr Ile Tyr Glu  Ser Gly Gly 
    1595                 1600                 1605             


Gly His  Asp Thr Ile Arg Ile  Asn Ala Gly Ala Asp  Gln Leu Trp 
    1610                 1615                 1620             


Phe Ala  Arg Gln Gly Asn Asp  Leu Glu Ile Arg Ile  Leu Gly Thr 
    1625                 1630                 1635             


Asp Asp  Ala Leu Thr Val His  Asp Trp Tyr Arg Asp  Ala Asp His 
    1640                 1645                 1650             


Arg Val  Glu Ile Ile His Ala  Ala Asn Gln Ala Val  Asp Gln Ala 
    1655                 1660                 1665             


Gly Ile  Glu Lys Leu Val Glu  Ala Met Ala Gln Tyr  Pro Asp Pro 
    1670                 1675                 1680             


Gly Ala  Ala Ala Ala Ala Pro  Pro Ala Ala Arg Val  Pro Asp Thr 
    1685                 1690                 1695             


Leu Met  Gln Ser Leu Ala Val  Asn Trp Arg 
    1700                 1705             


<210>  4
<211>  1720
<212>  PRT
<213>  ARTIFICIAL

<220>
<223>  CyaA/233OVA/E570Q+K860R/AC-

<400>  4

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Gly Ser Ile Asp 
            180                 185                 190         


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
        195                 200                 205             


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
    210                 215                 220                 


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Val Leu Ser Ile Ile Asn 
225                 230                 235                 240 


Phe Glu Lys Leu Val His Leu Asp Arg Glu Arg Ile Asp Leu Leu Trp 
                245                 250                 255     


Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu Ala Arg 
            260                 265                 270         


Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile Thr Asp 
        275                 280                 285             


Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His Ala Val 
    290                 295                 300                 


Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn Pro Phe 
305                 310                 315                 320 


Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly Glu Ser 
                325                 330                 335     


Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln Gln Arg 
            340                 345                 350         


Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val Ala Gly 
        355                 360                 365             


Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val Pro Ser 
    370                 375                 380                 


Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro Ala Ser 
385                 390                 395                 400 


Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln Asp Ser 
                405                 410                 415     


Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser Leu Gly 
            420                 425                 430         


Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu Met Thr 
        435                 440                 445             


Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu Pro Gly 
    450                 455                 460                 


Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln Gly Ala 
465                 470                 475                 480 


Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala Leu Met 
                485                 490                 495     


Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu Ala Ala 
            500                 505                 510         


Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser Ala Val 
        515                 520                 525             


Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp Ala Gly 
    530                 535                 540                 


Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly Ile Ala 
545                 550                 555                 560 


Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro Ala Gly 
                565                 570                 575     


Gln Lys Ala Ala Ala Gly Ala Gln Ile Ala Leu Gln Leu Thr Gly Gly 
            580                 585                 590         


Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala Ala Arg 
        595                 600                 605             


Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly Ala Ala 
    610                 615                 620                 


Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr Gly Leu 
625                 630                 635                 640 


Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala Gln Glu 
                645                 650                 655     


Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln Leu Tyr 
            660                 665                 670         


Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser Ala Val 
        675                 680                 685             


Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala Ser Val 
    690                 695                 700                 


Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly Ala Leu 
705                 710                 715                 720 


Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys Leu Ala 
                725                 730                 735     


Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln Ala Tyr 
            740                 745                 750         


Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn Ser Asp 
        755                 760                 765             


Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn Ala Ser 
    770                 775                 780                 


Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala Leu Glu 
785                 790                 795                 800 


Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val Asp Val 
                805                 810                 815     


Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln Pro Val 
            820                 825                 830         


Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg Lys Gly 
        835                 840                 845             


Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro Gly Glu 
    850                 855                 860                 


Glu Gln Arg Arg Arg Thr Lys Thr Gly Arg Ser Glu Phe Thr Thr Phe 
865                 870                 875                 880 


Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp Gly Ala 
                885                 890                 895     


Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu Val Asp 
            900                 905                 910         


Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile Gly Gly 
        915                 920                 925             


Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His Tyr Asp 
    930                 935                 940                 


Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly Arg Gln 
945                 950                 955                 960 


Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val Arg Lys 
                965                 970                 975     


Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr Gln Thr 
            980                 985                 990         


Thr Ala Tyr Gly Lys Arg Thr Glu  Asn Val Gln Tyr Arg  His Val Glu 
        995                 1000                 1005             


Leu Ala  Arg Val Gly Gln Val  Val Glu Val Asp Thr  Leu Glu His 
    1010                 1015                 1020             


Val Gln  His Ile Ile Gly Gly  Ala Gly Asn Asp Ser  Ile Thr Gly 
    1025                 1030                 1035             


Asn Ala  His Asp Asn Phe Leu  Ala Gly Gly Ser Gly  Asp Asp Arg 
    1040                 1045                 1050             


Leu Asp  Gly Gly Ala Gly Asn  Asp Thr Leu Val Gly  Gly Glu Gly 
    1055                 1060                 1065             


Gln Asn  Thr Val Ile Gly Gly  Ala Gly Asp Asp Val  Phe Leu Gln 
    1070                 1075                 1080             


Asp Leu  Gly Val Trp Ser Asn  Gln Leu Asp Gly Gly  Ala Gly Val 
    1085                 1090                 1095             


Asp Thr  Val Lys Tyr Asn Val  His Gln Pro Ser Glu  Glu Arg Leu 
    1100                 1105                 1110             


Glu Arg  Met Gly Asp Thr Gly  Ile His Ala Asp Leu  Gln Lys Gly 
    1115                 1120                 1125             


Thr Val  Glu Lys Trp Pro Ala  Leu Asn Leu Phe Ser  Val Asp His 
    1130                 1135                 1140             


Val Lys  Asn Ile Glu Asn Leu  His Gly Ser Arg Leu  Asn Asp Arg 
    1145                 1150                 1155             


Ile Ala  Gly Asp Asp Gln Asp  Asn Glu Leu Trp Gly  His Asp Gly 
    1160                 1165                 1170             


Asn Asp  Thr Ile Arg Gly Arg  Gly Gly Asp Asp Ile  Leu Arg Gly 
    1175                 1180                 1185             


Gly Leu  Gly Leu Asp Thr Leu  Tyr Gly Glu Asp Gly  Asn Asp Ile 
    1190                 1195                 1200             


Phe Leu  Gln Asp Asp Glu Thr  Val Ser Asp Asp Ile  Asp Gly Gly 
    1205                 1210                 1215             


Ala Gly  Leu Asp Thr Val Asp  Tyr Ser Ala Met Ile  His Pro Gly 
    1220                 1225                 1230             


Arg Ile  Val Ala Pro His Glu  Tyr Gly Phe Gly Ile  Glu Ala Asp 
    1235                 1240                 1245             


Leu Ser  Arg Glu Trp Val Arg  Lys Ala Ser Ala Leu  Gly Val Asp 
    1250                 1255                 1260             


Tyr Tyr  Asp Asn Val Arg Asn  Val Glu Asn Val Ile  Gly Thr Ser 
    1265                 1270                 1275             


Met Lys  Asp Val Leu Ile Gly  Asp Ala Gln Ala Asn  Thr Leu Met 
    1280                 1285                 1290             


Gly Gln  Gly Gly Asp Asp Thr  Val Arg Gly Gly Asp  Gly Asp Asp 
    1295                 1300                 1305             


Leu Leu  Phe Gly Gly Asp Gly  Asn Asp Met Leu Tyr  Gly Asp Ala 
    1310                 1315                 1320             


Gly Asn  Asp Thr Leu Tyr Gly  Gly Leu Gly Asp Asp  Thr Leu Glu 
    1325                 1330                 1335             


Gly Gly  Ala Gly Asn Asp Trp  Phe Gly Gln Thr Gln  Ala Arg Glu 
    1340                 1345                 1350             


His Asp  Val Leu Arg Gly Gly  Asp Gly Val Asp Thr  Val Asp Tyr 
    1355                 1360                 1365             


Ser Gln  Thr Gly Ala His Ala  Gly Ile Ala Ala Gly  Arg Ile Gly 
    1370                 1375                 1380             


Leu Gly  Ile Leu Ala Asp Leu  Gly Ala Gly Arg Val  Asp Lys Leu 
    1385                 1390                 1395             


Gly Glu  Ala Gly Ser Ser Ala  Tyr Asp Thr Val Ser  Gly Ile Glu 
    1400                 1405                 1410             


Asn Val  Val Gly Thr Glu Leu  Ala Asp Arg Ile Thr  Gly Asp Ala 
    1415                 1420                 1425             


Gln Ala  Asn Val Leu Arg Gly  Ala Gly Gly Ala Asp  Val Leu Ala 
    1430                 1435                 1440             


Gly Gly  Glu Gly Asp Asp Val  Leu Leu Gly Gly Asp  Gly Asp Asp 
    1445                 1450                 1455             


Gln Leu  Ser Gly Asp Ala Gly  Arg Asp Arg Leu Tyr  Gly Glu Ala 
    1460                 1465                 1470             


Gly Asp  Asp Trp Phe Phe Gln  Asp Ala Ala Asn Ala  Gly Asn Leu 
    1475                 1480                 1485             


Leu Asp  Gly Gly Asp Gly Arg  Asp Thr Val Asp Phe  Ser Gly Pro 
    1490                 1495                 1500             


Gly Arg  Gly Leu Asp Ala Gly  Ala Lys Gly Val Phe  Leu Ser Leu 
    1505                 1510                 1515             


Gly Lys  Gly Phe Ala Ser Leu  Met Asp Glu Pro Glu  Thr Ser Asn 
    1520                 1525                 1530             


Val Leu  Arg Asn Ile Glu Asn  Ala Val Gly Ser Ala  Arg Asp Asp 
    1535                 1540                 1545             


Val Leu  Ile Gly Asp Ala Gly  Ala Asn Val Leu Asn  Gly Leu Ala 
    1550                 1555                 1560             


Gly Asn  Asp Val Leu Ser Gly  Gly Ala Gly Asp Asp  Val Leu Leu 
    1565                 1570                 1575             


Gly Asp  Glu Gly Ser Asp Leu  Leu Ser Gly Asp Ala  Gly Asn Asp 
    1580                 1585                 1590             


Asp Leu  Phe Gly Gly Gln Gly  Asp Asp Thr Tyr Leu  Phe Gly Val 
    1595                 1600                 1605             


Gly Tyr  Gly His Asp Thr Ile  Tyr Glu Ser Gly Gly  Gly His Asp 
    1610                 1615                 1620             


Thr Ile  Arg Ile Asn Ala Gly  Ala Asp Gln Leu Trp  Phe Ala Arg 
    1625                 1630                 1635             


Gln Gly  Asn Asp Leu Glu Ile  Arg Ile Leu Gly Thr  Asp Asp Ala 
    1640                 1645                 1650             


Leu Thr  Val His Asp Trp Tyr  Arg Asp Ala Asp His  Arg Val Glu 
    1655                 1660                 1665             


Ile Ile  His Ala Ala Asn Gln  Ala Val Asp Gln Ala  Gly Ile Glu 
    1670                 1675                 1680             


Lys Leu  Val Glu Ala Met Ala  Gln Tyr Pro Asp Pro  Gly Ala Ala 
    1685                 1690                 1695             


Ala Ala  Ala Pro Pro Ala Ala  Arg Val Pro Asp Thr  Leu Met Gln 
    1700                 1705                 1710             


Ser Leu  Ala Val Asn Trp Arg  
    1715                 1720 


<210>  5
<211>  8825
<212>  DNA
<213>  Artificial

<220>
<223>  QR AC-

<400>  5
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca     60

gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga    120

gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt    180

gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat    240

ttaatacgac tcactatagg gaaagctcta gaaataattt tgtttaactt taagaaggag    300

atatacatat gcttccgtcc gcccaagcgc cctccctcct caatcccacc gacgacttcg    360

cggcactggg caatattgcc tggctgtgga tgaactctcc catgcaccgc gactggccgg    420

tgcatctgct cgcacgcaac acgctcgcgc cgattcaact gggccaatac attctgctgc    480

gatgcaatga cgtgccggtt gcatactgca gctgggccct aatggacgcc gacaccgaac    540

tctcctatgt catggcgccc tcgtcgctgg gcgggaatgc ctggaactgc ggcgaccgac    600

tgtggatcat cgactggatc gcgccattct cgcgcgacga caatcgtgcg ctgcgccgcg    660

cgctggccga acggcacccc gacagcgtgg gccgttcgct gcgcgttcgg cgcggcggcg    720

acaccgcgcg cgtcaaggag taccgaggcc gcgcgctgga cgcggccgcc actcgcgcgc    780

agctggaccg ctaccatgcc gaactgatcg caggactgcg cgcgagcaac ggcggatacg    840

cgccgcgagg ccggggcacc gcctaaggat cctctagagc ttgcatgccc tggcacgaca    900

ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc    960

attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga   1020

gcggataaca atttcacaca ggaaacagct atgaccatgc agcaatcgca tcaggctggt   1080

tacgcaaacg ccgccgaccg ggagtctggc atccccgcag ccgtactcga tggcatcaag   1140

gccgtggcga aggaaaaaaa cgccacattg atgttccgcc tggtcaaccc ccattccacc   1200

agcctgattg ccgaaggggt ggccaccaaa ggattgggcg tgcacgccaa gtcgtccgat   1260

tgggggttgc aggcgggcta cattcccgtc aacccgaatc tttccaaact gttcggccgt   1320

gcgcccgagg tgatcgcgcg ggccgacaac gacgtcaaca gcagcctggc gcatggccat   1380

accgcggtcg acctgacgct gtcgaaagag cggcttgact atctgcggca agcgggcctg   1440

gtcaccggca tggccgatgg cgtggtcgcg agcaaccacg caggctacga gcagttcgag   1500

tttcgcgtga aggaaacctc ggacgggcgc tatgccgtgc agtatcgccg caagggcggc   1560

gacgatttcg aggcggtcaa ggtgatcggc aatgccgccg gtattccact gacggcggat   1620

ggatccatcg acatgttcgc cattatgccg catctgtcca acttccgcga ctcggcgcgc   1680

agttcggtga ccagcggcga ttcggtgacc gattacctgg cgcgcacgcg gcgggccgcc   1740

agcgaggcca cgggcggtgt acacctggat cgcgaacgca tcgacttgtt gtggaaaatc   1800

gctcgcgccg gcgcccgttc cgcagtgggc accgaggcgc gtcgccagtt ccgctacgac   1860

ggcgacatga atatcggcgt gatcaccgat ttcgagctgg aagtgcgcaa tgcgctgaac   1920

aggcgggcgc acgccgtcgg cgcgcaggac gtggtccagc atggcactga gcagaacaat   1980

cctttcccgg aggcagatga gaagattttc gtcgtatcgg ccaccggtga aagccagatg   2040

ctcacgcgcg ggcaactgaa ggaatacatt ggccagcagc gcggcgaggg ctatgtcttc   2100

tacgagaacc gtgcatacgg cgtggcgggg aaaagcctgt tcgacgatgg gctgggagcc   2160

gcgcccggcg tgccgagcgg acgttcgaag ttctcgccgg atgtactgga aacggtgccg   2220

gcgtcacccg gattgcggcg gccgtcgctg ggcgcagtgg aacgccagga ttccggctat   2280

gacagccttg atggggtggg atcgcgatcg ttctcgttgg gcgaggtgtc cgacatggcc   2340

gccgtggaag cggcggaact ggaaatgacc cggcaagtct tgcacgccgg ggcgcggcag   2400

gacgatgccg agccgggcgt gagcggtgcg tcggcgcact gggggcagcg ggcgctgcag   2460

ggcgcccagg cggtggcggc ggcgcagcgg ctggttcatg ccattgccct gatgacgcaa   2520

ttcggccggg ccggttccac caacacgccg caggaagcgg cctcgttgtc ggcggccgtg   2580

ttcggcttgg gcgaggccag cagcgccgtg gccgaaaccg tgagcggttt tttccgcggg   2640

tcttcgcgct gggccggcgg tttcggcgtg gctggcggcg cgatggcgct gggaggcggc   2700

atcgccgcgg ccgttggcgc cgggatgtcg ttgaccgatg acgcgccggc cggacagaag   2760

gccgccgccg gagctccgat cgcgctgcag ttaacgggtg gaacggtcga gctggcttct   2820

tccatcgcgt tggcgctggc cgcggcgcgc ggcgtgacca gcggcttgca ggtggccggg   2880

gcgtcggccg gggcggctgc cggcgcattg gccgcggcgc tcagtcccat ggagatctac   2940

ggcctggtgc agcaatcgca ctatgcggat cagctggaca agctggcgca ggaatcgagc   3000

gcatacggtt acgagggcga cgccttgctg gcccagctgt atcgcgacaa gacggccgcc   3060

gagggcgccg tcgccggcgt ctccgccgtc ctgagcacgg tgggggcggc ggtgtcgatc   3120

gccgcggcgg ccagcgtggt aggggccccg gtggcggtgg tcacttcctt gctgaccggg   3180

gctctcaacg gcatcctgcg cggcgtgcag cagcccatca tcgaaaagct ggccaacgat   3240

tacgctcgca agatcgacga gctgggcggg ccgcaagcgt acttcgagaa aaacctgcag   3300

gcgcgtcacg aacaactggc caattcggac ggcctacgga aaatgctggc cgacctgcag   3360

gccggttgga acgccagcag cgtgatcggg gtgcagacga cagagatctc caagtcggcg   3420

ctcgaactgg ccgccattac cggcaacgcg gacaacctga aatccgtcga cgtgttcgtg   3480

gaccgcttcg tccagggcga gcgggtggcc ggccagccgg tggtcctcga cgtcgccgcc   3540

ggcggcatcg atatcgccag ccgcaagggc gagcggccgg cgctgacgtt catcacgccg   3600

ctggccgcgc caggagaaga gcagcgccgg cgcacgaaaa cgggcagatc tgaattcacc   3660

acattcgtcg agatcgtggg caagcaggac cgctggcgca tccgggacgg cgcggccgac   3720

accaccatcg atctggccaa ggtggtgtcg caactggtcg acgccaatgg cgtgctcaag   3780

cacagcatca aactggatgt gatcggcgga gatggcgatg acgtcgtgct tgccaatgct   3840

tcgcgcatcc attatgacgg cggcgcgggc accaacacgg tcagctatgc cgccctgggt   3900

cgacaggatt ccattaccgt gtccgccgac ggggaacgtt tcaacgtgcg caagcagttg   3960

aacaacgcca acgtgtatcg cgaaggcgtg gctacccaga caaccgccta cggcaagcgc   4020

acggagaatg tccaataccg ccatgtcgag ctggcccgtg tcgggcaagt ggtggaggtc   4080

gacacgctcg agcatgtgca gcacatcatc ggcggggccg gcaacgattc gatcaccggc   4140

aatgcgcacg acaacttcct agccggcggg tcgggcgacg acaggctgga tggcggcgcc   4200

ggcaacgaca ccctggttgg cggcgagggc caaaacacgg tcatcggcgg cgccggcgac   4260

gacgtattcc tgcaggacct gggggtatgg agcaaccagc tcgatggcgg cgcgggcgtc   4320

gataccgtga agtacaacgt gcaccagcct tccgaggagc gcctcgaacg catgggcgac   4380

acgggcatcc atgccgatct tcaaaagggc acggtcgaga agtggccggc cctgaacctg   4440

ttcagcgtcg accatgtcaa gaatatcgag aatctgcacg gctcccgcct aaacgaccgc   4500

atcgccggcg acgaccagga caacgagctc tggggccacg atggcaacga cacgatacgc   4560

ggccggggcg gcgacgacat cctgcgcggc ggcctgggcc tggacacgct gtatggcgag   4620

gacggcaacg acatcttcct gcaggacgac gagaccgtca gcgatgacat cgacggcggc   4680

gcggggctgg acaccgtcga ctactccgcc atgatccatc caggcaggat cgttgcgccg   4740

catgaatacg gcttcgggat cgaggcggac ctgtccaggg aatgggtgcg caaggcgtcc   4800

gcgctgggcg tggactatta cgataatgtc cgcaatgtcg aaaacgtcat cggtacgagc   4860

atgaaggatg tgctcatcgg cgacgcgcaa gccaataccc tgatgggcca gggcggcgac   4920

gataccgtgc gcggcggcga cggcgatgat ctgctgttcg gcggcgacgg caacgacatg   4980

ctgtatggcg acgccggcaa cgacaccctc tacggggggc tgggcgacga tacccttgaa   5040

ggcggcgcgg gcaacgattg gttcggccag acgcaggcgc gcgagcatga cgtgctgcgc   5100

ggcggagatg gggtggatac cgtcgattac agccagaccg gcgcgcatgc cggcattgcc   5160

gcgggtcgca tcgggctggg catcctggct gacctgggcg ccggccgcgt cgacaagctg   5220

ggcgaggccg gcagcagcgc ctacgatacg gtttccggta tcgagaacgt ggtgggcacg   5280

gaactggccg accgcatcac gggcgatgcg caggccaacg tgctgcgcgg cgcgggtggc   5340

gccgacgtgc ttgcgggcgg cgagggcgac gatgtgctgc tgggcggcga cggcgacgac   5400

cagctgtcgg gcgacgccgg acgcgatcgc ttgtacggcg aagccggtga cgactggttc   5460

ttccaggatg ccgccaatgc cggcaatctg ctcgacggcg gcgacggccg cgataccgtg   5520

gatttcagcg gcccgggccg gggcctcgac gccggcgcaa agggcgtatt cctgagcttg   5580

ggcaaggggt tcgccagcct gatggacgaa cccgaaacca gcaacgtgtt gcgcaatatc   5640

gagaacgccg tgggcagcgc gcgtgatgac gtgctgatcg gcgacgcagg cgccaacgtc   5700

ctcaatggcc tggcgggcaa cgacgtgctg tccggcggcg ctggcgacga tgtgctgctg   5760

ggcgacgagg gctcggacct gctcagcggc gatgcgggca acgacgatct gttcggcggg   5820

cagggcgatg atacttatct gttcggggtc gggtacgggc acgacacgat ctacgaatcg   5880

ggcggcggcc atgacaccat ccgcatcaac gcgggggcgg accagctgtg gttcgcgcgc   5940

cagggcaacg acctggagat ccgcattctc ggcaccgacg atgcacttac cgtgcacgac   6000

tggtatcgcg acgccgatca ccgggtggaa atcatccatg ccgccaacca ggcggtagac   6060

caggcaggca tcgaaaagct ggtcgaggca atggcgcagt atccggaccc cggcgcggcg   6120

gcggctgccc cgccggcggc gcgcgtgccg gacacgctga tgcagtccct ggctgtcaac   6180

tggcgctgaa gcgccgtgaa tcacggcccg cctgcctcgc gcggcggcgc cgtctctttg   6240

cgttcttctc cgaggtattt cccatcatga attcactggc cgtcgtttta caacgtcgtg   6300

actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca   6360

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga   6420

atggcgaatg ggaaattgta aacgttaata ttttgttaat attttgttaa aattcgcgtt   6480

aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta   6540

taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc   6600

actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg   6660

cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact   6720

aaatcggaac cctaaaggga tgccccgatt tagagcttga cggggaaagc cggcgaacgt   6780

ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc   6840

ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc   6900

aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca   6960

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa   7020

aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt   7080

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca   7140

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag   7200

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc   7260

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca   7320

gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt   7380

aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct   7440

gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt   7500

aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga   7560

caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact   7620

tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc   7680

acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga   7740

gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt   7800

agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga   7860

gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact   7920

ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga   7980

taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt   8040

agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca   8100

aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct   8160

ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta   8220

gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct   8280

aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc   8340

aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca   8400

gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga   8460

aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg   8520

aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt   8580

cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag   8640

cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt   8700

tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt   8760

tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga   8820

ggaag                                                               8825


<210>  6
<211>  8855
<212>  DNA
<213>  Artificial

<220>
<223>  OVA QR AC-

<400>  6
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca     60

gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga    120

gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt    180

gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat    240

ttaatacgac tcactatagg gaaagctcta gaaataattt tgtttaactt taagaaggag    300

atatacatat gcttccgtcc gcccaagcgc cctccctcct caatcccacc gacgacttcg    360

cggcactggg caatattgcc tggctgtgga tgaactctcc catgcaccgc gactggccgg    420

tgcatctgct cgcacgcaac acgctcgcgc cgattcaact gggccaatac attctgctgc    480

gatgcaatga cgtgccggtt gcatactgca gctgggccct aatggacgcc gacaccgaac    540

tctcctatgt catggcgccc tcgtcgctgg gcgggaatgc ctggaactgc ggcgaccgac    600

tgtggatcat cgactggatc gcgccattct cgcgcgacga caatcgtgcg ctgcgccgcg    660

cgctggccga acggcacccc gacagcgtgg gccgttcgct gcgcgttcgg cgcggcggcg    720

acaccgcgcg cgtcaaggag taccgaggcc gcgcgctgga cgcggccgcc actcgcgcgc    780

agctggaccg ctaccatgcc gaactgatcg caggactgcg cgcgagcaac ggcggatacg    840

cgccgcgagg ccggggcacc gcctaaggat cctctagagc ttgcatgccc tggcacgaca    900

ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc    960

attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga   1020

gcggataaca atttcacaca ggaaacagct atgaccatgc agcaatcgca tcaggctggt   1080

tacgcaaacg ccgccgaccg ggagtctggc atccccgcag ccgtactcga tggcatcaag   1140

gccgtggcga aggaaaaaaa cgccacattg atgttccgcc tggtcaaccc ccattccacc   1200

agcctgattg ccgaaggggt ggccaccaaa ggattgggcg tgcacgccaa gtcgtccgat   1260

tgggggttgc aggcgggcta cattcccgtc aacccgaatc tttccaaact gttcggccgt   1320

gcgcccgagg tgatcgcgcg ggccgacaac gacgtcaaca gcagcctggc gcatggccat   1380

accgcggtcg acctgacgct gtcgaaagag cggcttgact atctgcggca agcgggcctg   1440

gtcaccggca tggccgatgg cgtggtcgcg agcaaccacg caggctacga gcagttcgag   1500

tttcgcgtga aggaaacctc ggacgggcgc tatgccgtgc agtatcgccg caagggcggc   1560

gacgatttcg aggcggtcaa ggtgatcggc aatgccgccg gtattccact gacggcggat   1620

ggatccatcg acatgttcgc cattatgccg catctgtcca acttccgcga ctcggcgcgc   1680

agttcggtga ccagcggcga ttcggtgacc gattacctgg cgcgcacgcg gcgggccgcc   1740

agcgaggcca cgggcggtgt actctcaata attaatttcg aaaagcttgt acacctggat   1800

cgcgaacgca tcgacttgtt gtggaaaatc gctcgcgccg gcgcccgttc cgcagtgggc   1860

accgaggcgc gtcgccagtt ccgctacgac ggcgacatga atatcggcgt gatcaccgat   1920

ttcgagctgg aagtgcgcaa tgcgctgaac aggcgggcgc acgccgtcgg cgcgcaggac   1980

gtggtccagc atggcactga gcagaacaat cctttcccgg aggcagatga gaagattttc   2040

gtcgtatcgg ccaccggtga aagccagatg ctcacgcgcg ggcaactgaa ggaatacatt   2100

ggccagcagc gcggcgaggg ctatgtcttc tacgagaacc gtgcatacgg cgtggcgggg   2160

aaaagcctgt tcgacgatgg gctgggagcc gcgcccggcg tgccgagcgg acgttcgaag   2220

ttctcgccgg atgtactgga aacggtgccg gcgtcacccg gattgcggcg gccgtcgctg   2280

ggcgcagtgg aacgccagga ttccggctat gacagccttg atggggtggg atcgcgatcg   2340

ttctcgttgg gcgaggtgtc cgacatggcc gccgtggaag cggcggaact ggaaatgacc   2400

cggcaagtct tgcacgccgg ggcgcggcag gacgatgccg agccgggcgt gagcggtgcg   2460

tcggcgcact gggggcagcg ggcgctgcag ggcgcccagg cggtggcggc ggcgcagcgg   2520

ctggttcatg ccattgccct gatgacgcaa ttcggccggg ccggttccac caacacgccg   2580

caggaagcgg cctcgttgtc ggcggccgtg ttcggcttgg gcgaggccag cagcgccgtg   2640

gccgaaaccg tgagcggttt tttccgcggg tcttcgcgct gggccggcgg tttcggcgtg   2700

gctggcggcg cgatggcgct gggaggcggc atcgccgcgg ccgttggcgc cgggatgtcg   2760

ttgaccgatg acgcgccggc cggacagaag gccgccgccg gagctccgat cgcgctgcag   2820

ttaacgggtg gaacggtcga gctggcttct tccatcgcgt tggcgctggc cgcggcgcgc   2880

ggcgtgacca gcggcttgca ggtggccggg gcgtcggccg gggcggctgc cggcgcattg   2940

gccgcggcgc tcagtcccat ggagatctac ggcctggtgc agcaatcgca ctatgcggat   3000

cagctggaca agctggcgca ggaatcgagc gcatacggtt acgagggcga cgccttgctg   3060

gcccagctgt atcgcgacaa gacggccgcc gagggcgccg tcgccggcgt ctccgccgtc   3120

ctgagcacgg tgggggcggc ggtgtcgatc gccgcggcgg ccagcgtggt aggggccccg   3180

gtggcggtgg tcacttcctt gctgaccggg gctctcaacg gcatcctgcg cggcgtgcag   3240

cagcccatca tcgaaaagct ggccaacgat tacgctcgca agatcgacga gctgggcggg   3300

ccgcaagcgt acttcgagaa aaacctgcag gcgcgtcacg aacaactggc caattcggac   3360

ggcctacgga aaatgctggc cgacctgcag gccggttgga acgccagcag cgtgatcggg   3420

gtgcagacga cagagatctc caagtcggcg ctcgaactgg ccgccattac cggcaacgcg   3480

gacaacctga aatccgtcga cgtgttcgtg gaccgcttcg tccagggcga gcgggtggcc   3540

ggccagccgg tggtcctcga cgtcgccgcc ggcggcatcg atatcgccag ccgcaagggc   3600

gagcggccgg cgctgacgtt catcacgccg ctggccgcgc caggagaaga gcagcgccgg   3660

cgcacgaaaa cgggcagatc tgaattcacc acattcgtcg agatcgtggg caagcaggac   3720

cgctggcgca tccgggacgg cgcggccgac accaccatcg atctggccaa ggtggtgtcg   3780

caactggtcg acgccaatgg cgtgctcaag cacagcatca aactggatgt gatcggcgga   3840

gatggcgatg acgtcgtgct tgccaatgct tcgcgcatcc attatgacgg cggcgcgggc   3900

accaacacgg tcagctatgc cgccctgggt cgacaggatt ccattaccgt gtccgccgac   3960

ggggaacgtt tcaacgtgcg caagcagttg aacaacgcca acgtgtatcg cgaaggcgtg   4020

gctacccaga caaccgccta cggcaagcgc acggagaatg tccaataccg ccatgtcgag   4080

ctggcccgtg tcgggcaagt ggtggaggtc gacacgctcg agcatgtgca gcacatcatc   4140

ggcggggccg gcaacgattc gatcaccggc aatgcgcacg acaacttcct agccggcggg   4200

tcgggcgacg acaggctgga tggcggcgcc ggcaacgaca ccctggttgg cggcgagggc   4260

caaaacacgg tcatcggcgg cgccggcgac gacgtattcc tgcaggacct gggggtatgg   4320

agcaaccagc tcgatggcgg cgcgggcgtc gataccgtga agtacaacgt gcaccagcct   4380

tccgaggagc gcctcgaacg catgggcgac acgggcatcc atgccgatct tcaaaagggc   4440

acggtcgaga agtggccggc cctgaacctg ttcagcgtcg accatgtcaa gaatatcgag   4500

aatctgcacg gctcccgcct aaacgaccgc atcgccggcg acgaccagga caacgagctc   4560

tggggccacg atggcaacga cacgatacgc ggccggggcg gcgacgacat cctgcgcggc   4620

ggcctgggcc tggacacgct gtatggcgag gacggcaacg acatcttcct gcaggacgac   4680

gagaccgtca gcgatgacat cgacggcggc gcggggctgg acaccgtcga ctactccgcc   4740

atgatccatc caggcaggat cgttgcgccg catgaatacg gcttcgggat cgaggcggac   4800

ctgtccaggg aatgggtgcg caaggcgtcc gcgctgggcg tggactatta cgataatgtc   4860

cgcaatgtcg aaaacgtcat cggtacgagc atgaaggatg tgctcatcgg cgacgcgcaa   4920

gccaataccc tgatgggcca gggcggcgac gataccgtgc gcggcggcga cggcgatgat   4980

ctgctgttcg gcggcgacgg caacgacatg ctgtatggcg acgccggcaa cgacaccctc   5040

tacggggggc tgggcgacga tacccttgaa ggcggcgcgg gcaacgattg gttcggccag   5100

acgcaggcgc gcgagcatga cgtgctgcgc ggcggagatg gggtggatac cgtcgattac   5160

agccagaccg gcgcgcatgc cggcattgcc gcgggtcgca tcgggctggg catcctggct   5220

gacctgggcg ccggccgcgt cgacaagctg ggcgaggccg gcagcagcgc ctacgatacg   5280

gtttccggta tcgagaacgt ggtgggcacg gaactggccg accgcatcac gggcgatgcg   5340

caggccaacg tgctgcgcgg cgcgggtggc gccgacgtgc ttgcgggcgg cgagggcgac   5400

gatgtgctgc tgggcggcga cggcgacgac cagctgtcgg gcgacgccgg acgcgatcgc   5460

ttgtacggcg aagccggtga cgactggttc ttccaggatg ccgccaatgc cggcaatctg   5520

ctcgacggcg gcgacggccg cgataccgtg gatttcagcg gcccgggccg gggcctcgac   5580

gccggcgcaa agggcgtatt cctgagcttg ggcaaggggt tcgccagcct gatggacgaa   5640

cccgaaacca gcaacgtgtt gcgcaatatc gagaacgccg tgggcagcgc gcgtgatgac   5700

gtgctgatcg gcgacgcagg cgccaacgtc ctcaatggcc tggcgggcaa cgacgtgctg   5760

tccggcggcg ctggcgacga tgtgctgctg ggcgacgagg gctcggacct gctcagcggc   5820

gatgcgggca acgacgatct gttcggcggg cagggcgatg atacttatct gttcggggtc   5880

gggtacgggc acgacacgat ctacgaatcg ggcggcggcc atgacaccat ccgcatcaac   5940

gcgggggcgg accagctgtg gttcgcgcgc cagggcaacg acctggagat ccgcattctc   6000

ggcaccgacg atgcacttac cgtgcacgac tggtatcgcg acgccgatca ccgggtggaa   6060

atcatccatg ccgccaacca ggcggtagac caggcaggca tcgaaaagct ggtcgaggca   6120

atggcgcagt atccggaccc cggcgcggcg gcggctgccc cgccggcggc gcgcgtgccg   6180

gacacgctga tgcagtccct ggctgtcaac tggcgctgaa gcgccgtgaa tcacggcccg   6240

cctgcctcgc gcggcggcgc cgtctctttg cgttcttctc cgaggtattt cccatcatga   6300

attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta   6360

atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg   6420

atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggaaattgta aacgttaata   6480

ttttgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa   6540

ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt   6600

gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa   6660

agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag   6720

ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga tgccccgatt   6780

tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg   6840

agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc   6900

cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg   6960

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata   7020

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg   7080

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac   7140

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact   7200

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat   7260

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga   7320

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac   7380

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat   7440

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac   7500

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct   7560

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac   7620

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga   7680

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg   7740

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact   7800

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac   7860

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta   7920

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt   7980

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga   8040

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc   8100

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt   8160

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc   8220

gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc   8280

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg   8340

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg   8400

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga   8460

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc   8520

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg   8580

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg   8640

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt   8700

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc   8760

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg   8820

aacgaccgag cgcagcgagt cagtgagcga ggaag                              8855


<210>  7
<211>  1706
<212>  PRT
<213>  Bordetella parapertussis

<400>  7

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Gly Gly Arg Ser Lys Ser Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Val Gly Ala Glu Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Ala 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Ile Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Glu Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Lys Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Leu Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ala  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly His  Asn Thr Val Val Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Ser  Leu Asn Asp 
    1130                 1135                 1140             


Ser Ile  Ala Gly Asp Asp Arg  Asp Asn Glu Leu Trp  Gly Asp Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile His Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Ala 
    1205                 1210                 1215             


Gly Lys  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Glu Gly Trp Val  Arg Lys Ala Ala Arg  Arg Gly Met 
    1235                 1240                 1245             


Gly Tyr  Tyr Asp Ser Val Arg  Ser Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Pro Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Ala Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Ala Gly Ala His  Ala Gly Val Ala Thr  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Glu Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Asn Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg His Ile Glu  Asn Ala Val Gly Ser  Val Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Ala Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ala  Ile His Ala Ala Asn  Gln Ala Ile Asp Pro  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  8
<211>  1706
<212>  PRT
<213>  Bordetella hinzii

<400>  8

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Leu Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ser  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly Gln  Asn Thr Val Ile Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Arg  Leu Asn Asp 
    1130                 1135                 1140             


Arg Ile  Ala Gly Asp Asp Gln  Asp Asn Glu Leu Trp  Gly His Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile Arg Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Pro 
    1205                 1210                 1215             


Gly Arg  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Arg Glu Trp Val  Arg Lys Ala Ser Ala  Leu Gly Val 
    1235                 1240                 1245             


Asp Tyr  Tyr Asp Asn Val Arg  Asn Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Gln Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Asp Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Thr Gly Ala His  Ala Gly Ile Ala Ala  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Asp Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Arg Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg Asn Ile Glu  Asn Ala Val Gly Ser  Ala Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Val Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ile  Ile His Ala Ala Asn  Gln Ala Val Asp Gln  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  9
<211>  1705
<212>  PRT
<213>  Bordetella bronchiseptica

<400>  9

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Arg Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Gly Arg Arg Ser Lys Ser Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Gly Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro Ala 
545                 550                 555                 560 


Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln Leu Thr Gly 
                565                 570                 575     


Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala Ala 
            580                 585                 590         


Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly Ala 
        595                 600                 605             


Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr Gly 
    610                 615                 620                 


Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala Gln 
625                 630                 635                 640 


Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln Leu 
                645                 650                 655     


Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser Ala 
            660                 665                 670         


Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala Ser 
        675                 680                 685             


Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly Ala 
    690                 695                 700                 


Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys Leu 
705                 710                 715                 720 


Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln Ala 
                725                 730                 735     


Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn Ser 
            740                 745                 750         


Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn Ala 
        755                 760                 765             


Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala Leu 
    770                 775                 780                 


Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Ala Asp 
785                 790                 795                 800 


Val Phe Val Asp Arg Phe Ile Gln Gly Glu Arg Val Ala Gly Gln Pro 
                805                 810                 815     


Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg Lys 
            820                 825                 830         


Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro Gly 
        835                 840                 845             


Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr Thr 
    850                 855                 860                 


Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp Gly 
865                 870                 875                 880 


Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu Val 
                885                 890                 895     


Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Glu Val Ile Gly 
            900                 905                 910         


Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His Tyr 
        915                 920                 925             


Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly Arg 
    930                 935                 940                 


Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val Arg 
945                 950                 955                 960 


Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr Gln 
                965                 970                 975     


Lys Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His Val 
            980                 985                 990         


Glu Leu Ala Arg Val Gly Gln Leu  Val Glu Val Asp Thr  Leu Glu His 
        995                 1000                 1005             


Val Gln  His Ile Ile Gly Gly  Ala Gly Asn Asp Ser  Ile Thr Gly 
    1010                 1015                 1020             


Asn Ala  His Asp Asn Phe Leu  Ala Gly Gly Ala Gly  Asp Asp Arg 
    1025                 1030                 1035             


Leu Asp  Gly Gly Ala Gly Asn  Asp Thr Leu Val Gly  Gly Glu Gly 
    1040                 1045                 1050             


His Asn  Thr Val Val Gly Gly  Ala Gly Asp Asp Val  Phe Leu Gln 
    1055                 1060                 1065             


Asp Leu  Gly Val Trp Ser Asn  Gln Leu Asp Gly Gly  Ala Gly Val 
    1070                 1075                 1080             


Asp Thr  Val Lys Tyr Asn Val  His Gln Pro Ser Glu  Glu Arg Leu 
    1085                 1090                 1095             


Glu Arg  Met Gly Asp Thr Gly  Ile His Ala Asp Leu  Gln Lys Gly 
    1100                 1105                 1110             


Thr Val  Glu Lys Trp Pro Ala  Leu Asn Leu Phe Ser  Val Asp His 
    1115                 1120                 1125             


Val Lys  Asn Ile Glu Asn Leu  His Gly Ser Ser Leu  Asn Asp Ser 
    1130                 1135                 1140             


Ile Ala  Gly Asp Asp Arg Asp  Asn Glu Leu Trp Gly  Asp Asp Gly 
    1145                 1150                 1155             


Asn Asp  Thr Ile His Gly Arg  Gly Gly Asp Asp Ile  Leu Arg Gly 
    1160                 1165                 1170             


Gly Leu  Gly Leu Asp Thr Leu  Tyr Gly Glu Asp Gly  Asn Asp Ile 
    1175                 1180                 1185             


Phe Leu  Gln Asp Asp Glu Thr  Val Ser Asp Asp Ile  Asp Gly Gly 
    1190                 1195                 1200             


Ala Gly  Leu Asp Thr Val Asp  Tyr Ser Ala Met Ile  His Ala Gly 
    1205                 1210                 1215             


Lys Ile  Val Ala Pro His Glu  Tyr Gly Phe Gly Ile  Glu Ala Asp 
    1220                 1225                 1230             


Leu Ser  Glu Gly Trp Val Arg  Lys Ala Ala Arg Arg  Gly Met Asp 
    1235                 1240                 1245             


Tyr Tyr  Asp Ser Val Arg Ser  Val Glu Asn Val Ile  Gly Thr Ser 
    1250                 1255                 1260             


Met Lys  Asp Val Leu Ile Gly  Asp Ala Gln Ala Asn  Thr Leu Met 
    1265                 1270                 1275             


Gly Gln  Gly Gly Asp Asp Thr  Val Arg Gly Gly Asp  Gly Asp Asp 
    1280                 1285                 1290             


Leu Leu  Phe Gly Gly Asp Gly  Asn Asp Met Leu Tyr  Gly Asp Ala 
    1295                 1300                 1305             


Gly Asn  Asp Thr Leu Tyr Gly  Gly Leu Gly Asp Asp  Thr Leu Glu 
    1310                 1315                 1320             


Gly Gly  Ala Gly Asn Asp Trp  Phe Gly Gln Thr Pro  Ala Arg Glu 
    1325                 1330                 1335             


His Asp  Val Leu Arg Gly Gly  Ala Gly Val Asp Thr  Val Asp Tyr 
    1340                 1345                 1350             


Ser Gln  Ala Gly Ala His Ala  Gly Val Ala Thr Gly  Arg Ile Gly 
    1355                 1360                 1365             


Leu Gly  Ile Leu Ala Asp Leu  Gly Ala Gly Arg Val  Asp Lys Leu 
    1370                 1375                 1380             


Gly Glu  Ala Gly Ser Ser Ala  Tyr Asp Thr Val Ser  Gly Ile Glu 
    1385                 1390                 1395             


Asn Val  Val Gly Thr Glu Leu  Ala Asp Arg Ile Thr  Gly Asp Ala 
    1400                 1405                 1410             


Gln Ala  Asn Val Leu Arg Gly  Ala Gly Gly Ala Asp  Val Leu Ala 
    1415                 1420                 1425             


Gly Gly  Glu Gly Asp Asp Val  Leu Leu Gly Gly Asp  Gly Asp Asp 
    1430                 1435                 1440             


Gln Leu  Ser Gly Asp Ala Gly  Arg Asp Arg Leu Tyr  Gly Glu Ala 
    1445                 1450                 1455             


Gly Asp  Asp Trp Phe Phe Gln  Asp Ala Ala Asn Ala  Gly Asn Leu 
    1460                 1465                 1470             


Leu Asp  Gly Gly Asp Gly Asn  Asp Thr Val Asp Phe  Ser Gly Pro 
    1475                 1480                 1485             


Gly Arg  Gly Leu Asp Ala Gly  Ala Lys Gly Val Phe  Leu Ser Leu 
    1490                 1495                 1500             


Gly Lys  Gly Phe Ala Ser Leu  Met Asp Glu Pro Glu  Thr Ser Asn 
    1505                 1510                 1515             


Val Leu  Arg His Ile Glu Asn  Ala Val Gly Ser Val  Arg Asp Asp 
    1520                 1525                 1530             


Val Leu  Ile Gly Asp Ala Gly  Ala Asn Val Leu Asn  Gly Leu Ala 
    1535                 1540                 1545             


Gly Asn  Asp Val Leu Ser Gly  Gly Ala Gly Asp Asp  Val Leu Leu 
    1550                 1555                 1560             


Gly Asp  Glu Gly Ser Asp Leu  Leu Ser Gly Asp Ala  Gly Asn Asp 
    1565                 1570                 1575             


Asp Leu  Phe Gly Gly Gln Gly  Asp Asp Thr Tyr Leu  Phe Gly Ala 
    1580                 1585                 1590             


Gly Tyr  Gly His Asp Thr Ile  Tyr Glu Ser Gly Gly  Gly His Asp 
    1595                 1600                 1605             


Thr Ile  Arg Ile Asn Ala Gly  Ala Asp Gln Leu Trp  Phe Ala Arg 
    1610                 1615                 1620             


Gln Gly  Asn Asp Leu Glu Ile  Arg Ile Leu Gly Thr  Asp Asp Ala 
    1625                 1630                 1635             


Leu Thr  Val His Asp Trp Tyr  Arg Asp Ala Asp His  Arg Val Glu 
    1640                 1645                 1650             


Ala Ile  His Ala Ala Asn Gln  Ala Ile Asp Pro Ala  Gly Ile Glu 
    1655                 1660                 1665             


Lys Leu  Val Glu Ala Met Ala  Gln Tyr Pro Asp Pro  Gly Ala Ala 
    1670                 1675                 1680             


Ala Ala  Ala Pro Pro Ala Ala  Arg Val Pro Asp Thr  Leu Met Gln 
    1685                 1690                 1695             


Ser Leu  Ala Val Asn Trp Arg  
    1700                 1705 


