                         SEQUENCE LISTING

<110>  Codexis, Inc.
       Behrouzian, Behnaz
       McDaniel, Robert
       Zhang, Xiyun
       Clark, Louis
 
<120>  Engineered Biosynthesis of Fatty Alcohols

<130>  CX3-005WO1

<150>  US 61/180,534
<151>  2009-05-22

<160>  11    

<170>  PatentIn version 3.3

<210>  1
<211>  3595
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence including optimized codon sequence coding 
       for Nocardia NRRL5646 CAR peptide.

<400>  1
acaatctaga ggccagcctg gccataagga gatatacata tggccgttga ctcccctgac     60

gaacgtcttc agcgtcgtat tgcacaactg tttgccgagg atgaacaagt caaagccgca    120

cgtcctcttg aggcagtctc cgccgctgtc tccgcaccag gtatgcgtct tgcccaaatc    180

gcagcaactg tcatggccgg ttatgcagac cgtcctgctg ccggtcaacg tgcattcgaa    240

cttaatactg acgacgctac gggccgtact tctcttcgtt tgttgcctcg tttcgagact    300

atcacctacc gtgagctgtg gcagcgtgtg ggtgaagttg ctgccgcatg gcatcatgac    360

ccggagaatc ctctgcgtgc gggcgacttc gttgcgcttt tgggctttac cagcattgat    420

tacgctaccc tggaccttgc cgatattcac ttgggagcgg tgacagtccc ccttcaagca    480

tctgccgccg tcagccaatt gatcgctatt ttgacggaga ccagcccgcg tcttctggcc    540

agcacaccag aacatctgga tgccgcggtc gaatgtcttc tggccggtac aacccctgag    600

cgtttggttg tttttgacta ccatccagag gatgacgacc agcgtgccgc tttcgagtcc    660

gcgcgtcgtc gtttggccga cgccggttcc ctggtcatcg ttgagaccct tgatgccgtg    720

cgtgctcgtg gccgtgactt gcctgccgct ccattgtttg tgcccgacac ggatgatgat    780

cctttggcgc tgcttattta tactagcggt agcaccggta cacctaaggg tgcgatgtac    840

accaaccgtc ttgcggccac gatgtggcag ggaaattcca tgttgcaggg aaactctcaa    900

cgtgtcggta ttaacttgaa ctacatgcca atgagccata ttgccggtcg tatttccctg    960

ttcggtgtgc ttgcccgtgg aggcactgct tactttgccg cgaaatccga tatgtccacg   1020

ttgtttgagg acattggctt ggttcgtccg acggaaattt tctttgtccc tcgtgtctgt   1080

gatatggttt tccagcgtta tcagtctgag ctggatcgtc gttctgtcgc cggtgcagat   1140

ttggatacgc tggaccgtga agtgaaggcg gacttgcgtc agaactacct gggtggtcgt   1200

ttcttggttg ccgtggtggg cagcgctccc cttgccgcag aaatgaagac ctttatggag   1260

tctgtccttg atctgccctt gcacgacggt tacggttcca cggaagcagg cgcttccgtt   1320

ctgctggata accaaattca acgtccaccc gtgctggatt acaagctggt ggatgttcct   1380

gagctgggat actttcgtac tgatcgtcct cacccacgtg gtgaactgtt gctgaaggcc   1440

gaaacgacga ttcctggcta ctacaagcgt cctgaggtca ccgcggaaat tttcgatgag   1500

gacggttttt ataaaacagg cgacattgtc gccgaattgg agcatgatcg tcttgtctat   1560

gttgaccgtc gtaacaacgt cttgaagttg tcccagggtg agttcgttac agttgcgcac   1620

ttggaagcag tctttgcctc ctctcctctt atccgtcaaa ttttcatcta cggctcctct   1680

gagcgttcct atcttcttgc tgttattgtc ccaactgacg atgcgctgcg tggacgtgac   1740

accgccacgc tgaaatccgc actggcggaa tccatccaac gtatcgcaaa ggacgccaat   1800

ctgcaaccct acgaaattcc ccgtgatttt ctgatcgaaa ccgagccgtt tacaattgct   1860

aacggtcttt tgtctggtat tgccaaactg cttcgtccca acctgaagga gcgttacggc   1920

gctcaacttg agcaaatgta cacggacctg gccacaggcc aagccgatga gttgcttgcc   1980

ctgcgtcgtg aagccgctga tttgcctgtg cttgaaacag tgagccgtgc ggccaaagcc   2040

atgttgggtg tcgcatctgc ggacatgcgt cctgacgccc acttcaccga tttgggcggt   2100

gactccctta gcgccttgtc cttttccaat cttcttcatg aaatttttgg cgttgaagtt   2160

ccggtcggag tggtcgtgag cccggcaaac gagttgcgtg acttggctaa ctatattgaa   2220

gccgaacgta actctggtgc caagcgtcct acttttactt ctgttcacgg tggaggttcc   2280

gaaattcgtg ccgccgattt gactttggac aagtttatcg acgcccgtac gttggccgca   2340

gccgacagca tcccccatgc tccagtcccg gcccaaaccg ttcttctgac cggtgccaac   2400

ggttacctgg gtcgtttcct gtgtcttgag tggcttgagc gtctggataa aaccggcgga   2460

acactgattt gcgttgttcg tggttccgac gccgctgccg cccgtaaacg tttggatagc   2520

gcgttcgact ccggtgatcc tggtcttctg gagcactacc aacagttggc tgcccgtacg   2580

ctggaggttt tggctggaga catcggtgat ccgaatttgg gccttgatga tgctacgtgg   2640

caacgtctgg cggaaacggt ggatttgatc gttcatcctg ccgctttggt caaccatgtt   2700

ttgccatata cccagctgtt tggcccaaac gttgttggta ctgctgaaat cgttcgtctt   2760

gcgattaccg ctcgtcgtaa gccagtcacc tacttgtcca ccgtgggtgt cgcggaccaa   2820

gttgatcccg ccgagtacca agaggattcc gatgttcgtg agatgtccgc tgttcgtgtt   2880

gtccgtgagt cctatgccaa cggttacgga aactctaaat gggccggtga ggtgctgctg   2940

cgtgaagcac acgatctttg tggtctgcca gttgcggtgt tccgttccga catgatcttg   3000

gcccattctc gttatgctgg ccagcttaat gtccaagatg ttttcactcg tctgatcctg   3060

tccctggtgg ctactggtat cgccccttat tccttctatc gtactgacgc tgatggtaac   3120

cgtcagcgtg cacattacga tggtttgcca gctgacttca cggcggctgc tatcaccgct   3180

ttgggtattc aggccacaga aggattccgt acgtacgatg tgttgaatcc ttacgatgat   3240

ggtatcagcc tggacgagtt cgtggactgg cttgtggaat ccggacatcc gatccaacgt   3300

atcacggatt actccgattg gtttcatcgt ttcgagactg cgattcgtgc acttcctgaa   3360

aagcaacgtc aggcctccgt gttgccgctt ttggacgctt accgtaaccc ttgcccagcg   3420

gtgcgtggcg ccattctgcc tgccaaagag tttcaggctg cggttcaaac cgcgaagatt   3480

ggcccagaac aggacattcc tcacctttct gctcccctga ttgacaagta cgtgtctgat   3540

ctggagcttt tgcaattgtt gtaatgaggc caaactggcc accatcacca tcacc        3595


<210>  2
<211>  1174
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence from optimized codon sequence coding 
       for Nocardia NRRL5646 CAR peptide.

<400>  2

Met Ala Val Asp Ser Pro Asp Glu Arg Leu Gln Arg Arg Ile Ala Gln 
1               5                   10                  15      


Leu Phe Ala Glu Asp Glu Gln Val Lys Ala Ala Arg Pro Leu Glu Ala 
            20                  25                  30          


Val Ser Ala Ala Val Ser Ala Pro Gly Met Arg Leu Ala Gln Ile Ala 
        35                  40                  45              


Ala Thr Val Met Ala Gly Tyr Ala Asp Arg Pro Ala Ala Gly Gln Arg 
    50                  55                  60                  


Ala Phe Glu Leu Asn Thr Asp Asp Ala Thr Gly Arg Thr Ser Leu Arg 
65                  70                  75                  80  


Leu Leu Pro Arg Phe Glu Thr Ile Thr Tyr Arg Glu Leu Trp Gln Arg 
                85                  90                  95      


Val Gly Glu Val Ala Ala Ala Trp His His Asp Pro Glu Asn Pro Leu 
            100                 105                 110         


Arg Ala Gly Asp Phe Val Ala Leu Leu Gly Phe Thr Ser Ile Asp Tyr 
        115                 120                 125             


Ala Thr Leu Asp Leu Ala Asp Ile His Leu Gly Ala Val Thr Val Pro 
    130                 135                 140                 


Leu Gln Ala Ser Ala Ala Val Ser Gln Leu Ile Ala Ile Leu Thr Glu 
145                 150                 155                 160 


Thr Ser Pro Arg Leu Leu Ala Ser Thr Pro Glu His Leu Asp Ala Ala 
                165                 170                 175     


Val Glu Cys Leu Leu Ala Gly Thr Thr Pro Glu Arg Leu Val Val Phe 
            180                 185                 190         


Asp Tyr His Pro Glu Asp Asp Asp Gln Arg Ala Ala Phe Glu Ser Ala 
        195                 200                 205             


Arg Arg Arg Leu Ala Asp Ala Gly Ser Leu Val Ile Val Glu Thr Leu 
    210                 215                 220                 


Asp Ala Val Arg Ala Arg Gly Arg Asp Leu Pro Ala Ala Pro Leu Phe 
225                 230                 235                 240 


Val Pro Asp Thr Asp Asp Asp Pro Leu Ala Leu Leu Ile Tyr Thr Ser 
                245                 250                 255     


Gly Ser Thr Gly Thr Pro Lys Gly Ala Met Tyr Thr Asn Arg Leu Ala 
            260                 265                 270         


Ala Thr Met Trp Gln Gly Asn Ser Met Leu Gln Gly Asn Ser Gln Arg 
        275                 280                 285             


Val Gly Ile Asn Leu Asn Tyr Met Pro Met Ser His Ile Ala Gly Arg 
    290                 295                 300                 


Ile Ser Leu Phe Gly Val Leu Ala Arg Gly Gly Thr Ala Tyr Phe Ala 
305                 310                 315                 320 


Ala Lys Ser Asp Met Ser Thr Leu Phe Glu Asp Ile Gly Leu Val Arg 
                325                 330                 335     


Pro Thr Glu Ile Phe Phe Val Pro Arg Val Cys Asp Met Val Phe Gln 
            340                 345                 350         


Arg Tyr Gln Ser Glu Leu Asp Arg Arg Ser Val Ala Gly Ala Asp Leu 
        355                 360                 365             


Asp Thr Leu Asp Arg Glu Val Lys Ala Asp Leu Arg Gln Asn Tyr Leu 
    370                 375                 380                 


Gly Gly Arg Phe Leu Val Ala Val Val Gly Ser Ala Pro Leu Ala Ala 
385                 390                 395                 400 


Glu Met Lys Thr Phe Met Glu Ser Val Leu Asp Leu Pro Leu His Asp 
                405                 410                 415     


Gly Tyr Gly Ser Thr Glu Ala Gly Ala Ser Val Leu Leu Asp Asn Gln 
            420                 425                 430         


Ile Gln Arg Pro Pro Val Leu Asp Tyr Lys Leu Val Asp Val Pro Glu 
        435                 440                 445             


Leu Gly Tyr Phe Arg Thr Asp Arg Pro His Pro Arg Gly Glu Leu Leu 
    450                 455                 460                 


Leu Lys Ala Glu Thr Thr Ile Pro Gly Tyr Tyr Lys Arg Pro Glu Val 
465                 470                 475                 480 


Thr Ala Glu Ile Phe Asp Glu Asp Gly Phe Tyr Lys Thr Gly Asp Ile 
                485                 490                 495     


Val Ala Glu Leu Glu His Asp Arg Leu Val Tyr Val Asp Arg Arg Asn 
            500                 505                 510         


Asn Val Leu Lys Leu Ser Gln Gly Glu Phe Val Thr Val Ala His Leu 
        515                 520                 525             


Glu Ala Val Phe Ala Ser Ser Pro Leu Ile Arg Gln Ile Phe Ile Tyr 
    530                 535                 540                 


Gly Ser Ser Glu Arg Ser Tyr Leu Leu Ala Val Ile Val Pro Thr Asp 
545                 550                 555                 560 


Asp Ala Leu Arg Gly Arg Asp Thr Ala Thr Leu Lys Ser Ala Leu Ala 
                565                 570                 575     


Glu Ser Ile Gln Arg Ile Ala Lys Asp Ala Asn Leu Gln Pro Tyr Glu 
            580                 585                 590         


Ile Pro Arg Asp Phe Leu Ile Glu Thr Glu Pro Phe Thr Ile Ala Asn 
        595                 600                 605             


Gly Leu Leu Ser Gly Ile Ala Lys Leu Leu Arg Pro Asn Leu Lys Glu 
    610                 615                 620                 


Arg Tyr Gly Ala Gln Leu Glu Gln Met Tyr Thr Asp Leu Ala Thr Gly 
625                 630                 635                 640 


Gln Ala Asp Glu Leu Leu Ala Leu Arg Arg Glu Ala Ala Asp Leu Pro 
                645                 650                 655     


Val Leu Glu Thr Val Ser Arg Ala Ala Lys Ala Met Leu Gly Val Ala 
            660                 665                 670         


Ser Ala Asp Met Arg Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp 
        675                 680                 685             


Ser Leu Ser Ala Leu Ser Phe Ser Asn Leu Leu His Glu Ile Phe Gly 
    690                 695                 700                 


Val Glu Val Pro Val Gly Val Val Val Ser Pro Ala Asn Glu Leu Arg 
705                 710                 715                 720 


Asp Leu Ala Asn Tyr Ile Glu Ala Glu Arg Asn Ser Gly Ala Lys Arg 
                725                 730                 735     


Pro Thr Phe Thr Ser Val His Gly Gly Gly Ser Glu Ile Arg Ala Ala 
            740                 745                 750         


Asp Leu Thr Leu Asp Lys Phe Ile Asp Ala Arg Thr Leu Ala Ala Ala 
        755                 760                 765             


Asp Ser Ile Pro His Ala Pro Val Pro Ala Gln Thr Val Leu Leu Thr 
    770                 775                 780                 


Gly Ala Asn Gly Tyr Leu Gly Arg Phe Leu Cys Leu Glu Trp Leu Glu 
785                 790                 795                 800 


Arg Leu Asp Lys Thr Gly Gly Thr Leu Ile Cys Val Val Arg Gly Ser 
                805                 810                 815     


Asp Ala Ala Ala Ala Arg Lys Arg Leu Asp Ser Ala Phe Asp Ser Gly 
            820                 825                 830         


Asp Pro Gly Leu Leu Glu His Tyr Gln Gln Leu Ala Ala Arg Thr Leu 
        835                 840                 845             


Glu Val Leu Ala Gly Asp Ile Gly Asp Pro Asn Leu Gly Leu Asp Asp 
    850                 855                 860                 


Ala Thr Trp Gln Arg Leu Ala Glu Thr Val Asp Leu Ile Val His Pro 
865                 870                 875                 880 


Ala Ala Leu Val Asn His Val Leu Pro Tyr Thr Gln Leu Phe Gly Pro 
                885                 890                 895     


Asn Val Val Gly Thr Ala Glu Ile Val Arg Leu Ala Ile Thr Ala Arg 
            900                 905                 910         


Arg Lys Pro Val Thr Tyr Leu Ser Thr Val Gly Val Ala Asp Gln Val 
        915                 920                 925             


Asp Pro Ala Glu Tyr Gln Glu Asp Ser Asp Val Arg Glu Met Ser Ala 
    930                 935                 940                 


Val Arg Val Val Arg Glu Ser Tyr Ala Asn Gly Tyr Gly Asn Ser Lys 
945                 950                 955                 960 


Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu 
                965                 970                 975     


Pro Val Ala Val Phe Arg Ser Asp Met Ile Leu Ala His Ser Arg Tyr 
            980                 985                 990         


Ala Gly Gln Leu Asn Val Gln Asp  Val Phe Thr Arg Leu  Ile Leu Ser 
        995                 1000                 1005             


Leu Val  Ala Thr Gly Ile Ala  Pro Tyr Ser Phe Tyr  Arg Thr Asp 
    1010                 1015                 1020             


Ala Asp  Gly Asn Arg Gln Arg  Ala His Tyr Asp Gly  Leu Pro Ala 
    1025                 1030                 1035             


Asp Phe  Thr Ala Ala Ala Ile  Thr Ala Leu Gly Ile  Gln Ala Thr 
    1040                 1045                 1050             


Glu Gly  Phe Arg Thr Tyr Asp  Val Leu Asn Pro Tyr  Asp Asp Gly 
    1055                 1060                 1065             


Ile Ser  Leu Asp Glu Phe Val  Asp Trp Leu Val Glu  Ser Gly His 
    1070                 1075                 1080             


Pro Ile  Gln Arg Ile Thr Asp  Tyr Ser Asp Trp Phe  His Arg Phe 
    1085                 1090                 1095             


Glu Thr  Ala Ile Arg Ala Leu  Pro Glu Lys Gln Arg  Gln Ala Ser 
    1100                 1105                 1110             


Val Leu  Pro Leu Leu Asp Ala  Tyr Arg Asn Pro Cys  Pro Ala Val 
    1115                 1120                 1125             


Arg Gly  Ala Ile Leu Pro Ala  Lys Glu Phe Gln Ala  Ala Val Gln 
    1130                 1135                 1140             


Thr Ala  Lys Ile Gly Pro Glu  Gln Asp Ile Pro His  Leu Ser Ala 
    1145                 1150                 1155             


Pro Leu  Ile Asp Lys Tyr Val  Ser Asp Leu Glu Leu  Leu Gln Leu 
    1160                 1165                 1170             


Leu 
    


<210>  3
<211>  3525
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence including optimized codon sequence coding 
       for Mycobacterium sp. (strain JLS) CAR peptide.

<400>  3
atgtccactg agacccgtga agcccgtttg cagcaacgta ttgctcactt gtttgccacc     60

gacccccaat ttgccgccgc ccgtcccgac cctcgtattt ctgacgccgt tgatcgtgac    120

gacgcacgtt tgaccgccat tgtgtctgct gtgatgtctg gctatgcaga tcgtcctgct    180

cttggtcaac gtgcagcaga gttcgctact gacccccaga ctggtcgtac tacgatggaa    240

ctgttgcctc gttttgacac gattacctac cgtgagttgc tggatcgtgt gcgtgccctt    300

accaacgcct ggcatgctga cggtgttcgt cctggagacc gtgttgcgat tttgggcttt    360

accggtattg attacactgt tgttgacctt gccttgattc agttgggtgc agtcgcagtc    420

ccattgcaaa ccagcgccgc cgttgaagcc cttcgtccca ttgttgctga aacagaaccc    480

atgttgattg ccaccggagt tgatcatgtt gatgccgccg ccgagcttgc tcttacaggt    540

caccgtccga gccaggttgt tgtgtttgac catcgtgaac aagttgatga cgaacgtgac    600

gctgtgcgtg ccgctaccgc acgtttgggt gacgcagttc cggtggagac tttggcagaa    660

gtcttgcgtc gtggtgccca tctgcccgct gtcgcacccc acgtctttga cgaggccgat    720

cctttgcgtt tgctgattta cacctctggc tctaccggcg ctccgaaggg tgcgatgtac    780

ccagagagca aagtcgcagg catgtggcgt gcaagcgcaa aggctgcctg gaacaatgat    840

cagacggcaa ttccgtctat cacgcttaac ttcttgccga tgtctcacgt catgggccgt    900

ggattgctgt gtggtactct ttctactggc ggtactgcgt attttgccgc acgttccgac    960

ctgtccacac tgcttgagga cttgcgtctt gtccgtccca cccaattgtc cttcgttccg   1020

cgtatttggg acatgctttt tcaggagttt gtcggtgaag tcgatcgtcg tgttaacgac   1080

ggtgcggacc gtcccactgc tgaggctgat gtcttggccg agttgcgtca ggaactgttg   1140

ggaggtcgtt ttgtcaccgc gatgaccggt agcgccccta tttcccctga gatgaaaacg   1200

tgggtggaaa cgcttcttga catgcacctt gttgaaggct atggttctac ggaggctggc   1260

gctgtgttcg tggacggtca cattcaacgt cccccggttt tggattacaa acttgttgac   1320

gttccagacc tgggttactt tagcacagac cgtccacatc cgcgtggtga gctgcttgtt   1380

cgttctacgc agttgtttcc aggatactac aaacgtccag acgtgaccgc cgaggttttc   1440

gatgatgatg gcttctaccg tactggagat attgtggctg aattgggtcc tgaccagttg   1500

cagtacctgg accgtcgtaa taatgtcctg aaattggcgc agggcgagtt cgtcactatc   1560

agcaaactgg aagctgtgtt tgccggttcc gccctggtcc gtcagatctt tgtttatggc   1620

aactccgccc gttcttactt gttggccgtc gttgttccca ctgatgacgc cgttgcacgt   1680

cacgatcctg cctccctgaa gacagctatt agcgcttctt tgcagcaggc tgcgaaaaca   1740

gcaggcttgc agagctatga attgcctcgt gactttttgg tggagacaca accttttaca   1800

ctggaaaacg gactgctgac gggtattcgt aagttggcac gtcctaagtt gaaagcgcgt   1860

tacggtgacc gtctggaggc cttgtatgtt gagcttgcag aaggccaggc aggtgaactg   1920

cgtactctgc gtcgtgatgg tgcaaaacgt cctgtcgccg agacagttgg ccgtgccgcc   1980

gccgccttgt tgggtgcagc tgcggcggat gtgcgtccag atgcccattt caccgatctt   2040

ggcggcgact ctctgtccgc cctgactttt ggtaatttgt tgcaggaaat cttcggtgtt   2100

gacgttcccg tcggcgtcat tgtctccccc gctgctgact tggcctccat cgctgcgtat   2160

attgaaacag agcaggcttc cacgggtaaa cgtccaactt atgcctccgt tcatggtcgt   2220

gatgctgagc aagtccgtgc ccgtgacttg acccttgata aattcatcga cgcagagacg   2280

ttgtctgcgg cgacagagtt gccagtgcca atcggtgaag tgcgtaccgt gctgcttaca   2340

ggcgctactg gctttctggg tcgttacctg gccctggatt ggctggaacg tatggcactg   2400

gttgatggca aagtgatctg cttggtccgt gcaaaagacg acgcagctgc gcgtaagcgt   2460

ctggatgaca ccttcgattc cggtgaccct aaattgttgg ctcattaccg taagttggcc   2520

gctgatcacc tggaggtctt ggcgggcgac aagggcgaag ccgatttggg tctgccacac   2580

caggtgtggc aacgtttggc tgacaccgtc gatcttatcg tggaccccgc tgcgttggtc   2640

aatcatgtgc tgccgtactc tcaacttttc ggacccaacg ccctgggaac ggcagagttg   2700

atccgtcttg ccttgacgac ccgtatcaaa cctttcacct acgtttccac cattggtgtt   2760

ggcgcgggta ttgagccggg tcgtttcaca gaagacgacg acattcgtgt tattagccct   2820

actcgtgccg ttgatacggg ttacgctaac ggatatggta actctaagtg ggcaggtgag   2880

gttcttcttc gtgaggccca cgatctttgt ggtctgccag ttgcagtttt tcgttgtgat   2940

atgattctgg ccgatacaac gtatgccggt caactgaacc tgccagatat gtttacccgt   3000

atgatggtct ctttggtgac aaccggcatt gccccgaagt cttttcatcc acttgatgcg   3060

aagggccacc gtcagcgtgc ccattatgac ggtttgccag tggaatttgt cgctgaaagc   3120

atctctgccc tgggtgccca ggctgtggac gaggctggca ctggtttcgc cacataccat   3180

gttatgaacc ctcatgatga cggcattggc cttgatgaat ttgttgattg gttggttgaa   3240

gcgggttatc gtatcgaccg tattgacgac tatgcagcct ggcttcaacg ttttgaaacc   3300

gctctgcgtg cactgcctga gcgtacacgt cagtactcct tgctgccgtt gcttcataat   3360

taccagcgtc ccgctcatcc aatcaacggt gctatggccc ccacggaccg tttccgtgcg   3420

gcagttcagg aggctaagtt gggtcctgac aaagacattc cgcatgttac tcctggtgtc   3480

atcgttaagt acgccacaga tttggaattg cttggcctga tttaa                   3525


<210>  4
<211>  1174
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence from optimized codon sequence coding 
       for Mycobacterium sp. (strain JLS) CAR peptide.

<400>  4

Met Ser Thr Glu Thr Arg Glu Ala Arg Leu Gln Gln Arg Ile Ala His 
1               5                   10                  15      


Leu Phe Ala Thr Asp Pro Gln Phe Ala Ala Ala Arg Pro Asp Pro Arg 
            20                  25                  30          


Ile Ser Asp Ala Val Asp Arg Asp Asp Ala Arg Leu Thr Ala Ile Val 
        35                  40                  45              


Ser Ala Val Met Ser Gly Tyr Ala Asp Arg Pro Ala Leu Gly Gln Arg 
    50                  55                  60                  


Ala Ala Glu Phe Ala Thr Asp Pro Gln Thr Gly Arg Thr Thr Met Glu 
65                  70                  75                  80  


Leu Leu Pro Arg Phe Asp Thr Ile Thr Tyr Arg Glu Leu Leu Asp Arg 
                85                  90                  95      


Val Arg Ala Leu Thr Asn Ala Trp His Ala Asp Gly Val Arg Pro Gly 
            100                 105                 110         


Asp Arg Val Ala Ile Leu Gly Phe Thr Gly Ile Asp Tyr Thr Val Val 
        115                 120                 125             


Asp Leu Ala Leu Ile Gln Leu Gly Ala Val Ala Val Pro Leu Gln Thr 
    130                 135                 140                 


Ser Ala Ala Val Glu Ala Leu Arg Pro Ile Val Ala Glu Thr Glu Pro 
145                 150                 155                 160 


Met Leu Ile Ala Thr Gly Val Asp His Val Asp Ala Ala Ala Glu Leu 
                165                 170                 175     


Ala Leu Thr Gly His Arg Pro Ser Gln Val Val Val Phe Asp His Arg 
            180                 185                 190         


Glu Gln Val Asp Asp Glu Arg Asp Ala Val Arg Ala Ala Thr Ala Arg 
        195                 200                 205             


Leu Gly Asp Ala Val Pro Val Glu Thr Leu Ala Glu Val Leu Arg Arg 
    210                 215                 220                 


Gly Ala His Leu Pro Ala Val Ala Pro His Val Phe Asp Glu Ala Asp 
225                 230                 235                 240 


Pro Leu Arg Leu Leu Ile Tyr Thr Ser Gly Ser Thr Gly Ala Pro Lys 
                245                 250                 255     


Gly Ala Met Tyr Pro Glu Ser Lys Val Ala Gly Met Trp Arg Ala Ser 
            260                 265                 270         


Ala Lys Ala Ala Trp Asn Asn Asp Gln Thr Ala Ile Pro Ser Ile Thr 
        275                 280                 285             


Leu Asn Phe Leu Pro Met Ser His Val Met Gly Arg Gly Leu Leu Cys 
    290                 295                 300                 


Gly Thr Leu Ser Thr Gly Gly Thr Ala Tyr Phe Ala Ala Arg Ser Asp 
305                 310                 315                 320 


Leu Ser Thr Leu Leu Glu Asp Leu Arg Leu Val Arg Pro Thr Gln Leu 
                325                 330                 335     


Ser Phe Val Pro Arg Ile Trp Asp Met Leu Phe Gln Glu Phe Val Gly 
            340                 345                 350         


Glu Val Asp Arg Arg Val Asn Asp Gly Ala Asp Arg Pro Thr Ala Glu 
        355                 360                 365             


Ala Asp Val Leu Ala Glu Leu Arg Gln Glu Leu Leu Gly Gly Arg Phe 
    370                 375                 380                 


Val Thr Ala Met Thr Gly Ser Ala Pro Ile Ser Pro Glu Met Lys Thr 
385                 390                 395                 400 


Trp Val Glu Thr Leu Leu Asp Met His Leu Val Glu Gly Tyr Gly Ser 
                405                 410                 415     


Thr Glu Ala Gly Ala Val Phe Val Asp Gly His Ile Gln Arg Pro Pro 
            420                 425                 430         


Val Leu Asp Tyr Lys Leu Val Asp Val Pro Asp Leu Gly Tyr Phe Ser 
        435                 440                 445             


Thr Asp Arg Pro His Pro Arg Gly Glu Leu Leu Val Arg Ser Thr Gln 
    450                 455                 460                 


Leu Phe Pro Gly Tyr Tyr Lys Arg Pro Asp Val Thr Ala Glu Val Phe 
465                 470                 475                 480 


Asp Asp Asp Gly Phe Tyr Arg Thr Gly Asp Ile Val Ala Glu Leu Gly 
                485                 490                 495     


Pro Asp Gln Leu Gln Tyr Leu Asp Arg Arg Asn Asn Val Leu Lys Leu 
            500                 505                 510         


Ala Gln Gly Glu Phe Val Thr Ile Ser Lys Leu Glu Ala Val Phe Ala 
        515                 520                 525             


Gly Ser Ala Leu Val Arg Gln Ile Phe Val Tyr Gly Asn Ser Ala Arg 
    530                 535                 540                 


Ser Tyr Leu Leu Ala Val Val Val Pro Thr Asp Asp Ala Val Ala Arg 
545                 550                 555                 560 


His Asp Pro Ala Ser Leu Lys Thr Ala Ile Ser Ala Ser Leu Gln Gln 
                565                 570                 575     


Ala Ala Lys Thr Ala Gly Leu Gln Ser Tyr Glu Leu Pro Arg Asp Phe 
            580                 585                 590         


Leu Val Glu Thr Gln Pro Phe Thr Leu Glu Asn Gly Leu Leu Thr Gly 
        595                 600                 605             


Ile Arg Lys Leu Ala Arg Pro Lys Leu Lys Ala Arg Tyr Gly Asp Arg 
    610                 615                 620                 


Leu Glu Ala Leu Tyr Val Glu Leu Ala Glu Gly Gln Ala Gly Glu Leu 
625                 630                 635                 640 


Arg Thr Leu Arg Arg Asp Gly Ala Lys Arg Pro Val Ala Glu Thr Val 
                645                 650                 655     


Gly Arg Ala Ala Ala Ala Leu Leu Gly Ala Ala Ala Ala Asp Val Arg 
            660                 665                 670         


Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala Leu 
        675                 680                 685             


Thr Phe Gly Asn Leu Leu Gln Glu Ile Phe Gly Val Asp Val Pro Val 
    690                 695                 700                 


Gly Val Ile Val Ser Pro Ala Ala Asp Leu Ala Ser Ile Ala Ala Tyr 
705                 710                 715                 720 


Ile Glu Thr Glu Gln Ala Ser Thr Gly Lys Arg Pro Thr Tyr Ala Ser 
                725                 730                 735     


Val His Gly Arg Asp Ala Glu Gln Val Arg Ala Arg Asp Leu Thr Leu 
            740                 745                 750         


Asp Lys Phe Ile Asp Ala Glu Thr Leu Ser Ala Ala Thr Glu Leu Pro 
        755                 760                 765             


Val Pro Ile Gly Glu Val Arg Thr Val Leu Leu Thr Gly Ala Thr Gly 
    770                 775                 780                 


Phe Leu Gly Arg Tyr Leu Ala Leu Asp Trp Leu Glu Arg Met Ala Leu 
785                 790                 795                 800 


Val Asp Gly Lys Val Ile Cys Leu Val Arg Ala Lys Asp Asp Ala Ala 
                805                 810                 815     


Ala Arg Lys Arg Leu Asp Asp Thr Phe Asp Ser Gly Asp Pro Lys Leu 
            820                 825                 830         


Leu Ala His Tyr Arg Lys Leu Ala Ala Asp His Leu Glu Val Leu Ala 
        835                 840                 845             


Gly Asp Lys Gly Glu Ala Asp Leu Gly Leu Pro His Gln Val Trp Gln 
    850                 855                 860                 


Arg Leu Ala Asp Thr Val Asp Leu Ile Val Asp Pro Ala Ala Leu Val 
865                 870                 875                 880 


Asn His Val Leu Pro Tyr Ser Gln Leu Phe Gly Pro Asn Ala Leu Gly 
                885                 890                 895     


Thr Ala Glu Leu Ile Arg Leu Ala Leu Thr Thr Arg Ile Lys Pro Phe 
            900                 905                 910         


Thr Tyr Val Ser Thr Ile Gly Val Gly Ala Gly Ile Glu Pro Gly Arg 
        915                 920                 925             


Phe Thr Glu Asp Asp Asp Ile Arg Val Ile Ser Pro Thr Arg Ala Val 
    930                 935                 940                 


Asp Thr Gly Tyr Ala Asn Gly Tyr Gly Asn Ser Lys Trp Ala Gly Glu 
945                 950                 955                 960 


Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu Pro Val Ala Val 
                965                 970                 975     


Phe Arg Cys Asp Met Ile Leu Ala Asp Thr Thr Tyr Ala Gly Gln Leu 
            980                 985                 990         


Asn Leu Pro Asp Met Phe Thr Arg  Met Met Val Ser Leu  Val Thr Thr 
        995                 1000                 1005             


Gly Ile  Ala Pro Lys Ser Phe  His Pro Leu Asp Ala  Lys Gly His 
    1010                 1015                 1020             


Arg Gln  Arg Ala His Tyr Asp  Gly Leu Pro Val Glu  Phe Val Ala 
    1025                 1030                 1035             


Glu Ser  Ile Ser Ala Leu Gly  Ala Gln Ala Val Asp  Glu Ala Gly 
    1040                 1045                 1050             


Thr Gly  Phe Ala Thr Tyr His  Val Met Asn Pro His  Asp Asp Gly 
    1055                 1060                 1065             


Ile Gly  Leu Asp Glu Phe Val  Asp Trp Leu Val Glu  Ala Gly Tyr 
    1070                 1075                 1080             


Arg Ile  Asp Arg Ile Asp Asp  Tyr Ala Ala Trp Leu  Gln Arg Phe 
    1085                 1090                 1095             


Glu Thr  Ala Leu Arg Ala Leu  Pro Glu Arg Thr Arg  Gln Tyr Ser 
    1100                 1105                 1110             


Leu Leu  Pro Leu Leu His Asn  Tyr Gln Arg Pro Ala  His Pro Ile 
    1115                 1120                 1125             


Asn Gly  Ala Met Ala Pro Thr  Asp Arg Phe Arg Ala  Ala Val Gln 
    1130                 1135                 1140             


Glu Ala  Lys Leu Gly Pro Asp  Lys Asp Ile Pro His  Val Thr Pro 
    1145                 1150                 1155             


Gly Val  Ile Val Lys Tyr Ala  Thr Asp Leu Glu Leu  Leu Gly Leu 
    1160                 1165                 1170             


Ile 
    


<210>  5
<211>  3517
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence including optimized codon sequence coding 
       for Streptomyces griseus CAR peptide.

<400>  5
acaatctaga ggccagcctg gccataagga gatatacata tggctgaacc ccttgatgcc     60

gcaaccgcct ccgcacacga ccctggacaa ggtttggcag aagcccttgc cgccgtggaa    120

cctggtcgtg cccttgctga agttatggct tccgttttgg aaggtcacgg tgatcgtccg    180

gctttgggcg agcgtgctcg tgaacccgaa actggacgtt tgttgcctca ttttgatacg    240

atctcctatc gtgagctttg gtctcgtgtg cgtgctttgg ccggtcgttg gcatcatgac    300

cctgagtacc ctctgggtcc cggagaccgt atctgcaccc tgggctttac cagcacagat    360

tatgccaccc tggatcttgc ttgcatccac ctgggtgctg ttccagttcc attgccatcc    420

aacgctccat tgccccgttt ggcgccggtg gttgaggagt ccggcccaac cgttcttgct    480

gcatccgttg atcgtttgga tactgcgatt gatgttgtcc tggccagctc taccatccgt    540

cgtctgttgg tcttcgatga tggacctggt gccacccgtc caggaggtgc ccttgccgca    600

gcccgtcaac gtctgtccgg ttccccggtc accgtggaca ctctggccgg tcttatcgac    660

cgtggccgtg accttccccc cccacccctt tatattcctg atcctggcga ggaccctctg    720

gctctgctga tttacacgtc cggatctaca ggcgcaccaa aaggcgcaat gtacactcaa    780

cgtctgctgg gtacagcatg gtacggtttc agctacggcg ccgccgatac ccctgccatt    840

tccgttctgt atctgccaca gtcccacctg gctggtcgtt acgctgtcat gggtagcttg    900

gtgaaaggtg gtactggata ctttacggca gcggatgact tgtccaccct tttcgaagac    960

attgcgcttg tccgtcccac cgaattgacg atggttcctc gtctgtgtga catgcttctg   1020

caacactatc gttctgagcg tgatcgtcgt gcggacgagc ccggtgatat tgaagctgcc   1080

gtcactaaag ccgttcgtga ggacttcctg ggtggacgtg tggcgaaggc tttcgttggt   1140

actgcccctc tttccgccga gttgacggct ttcgtcgaat ccgtgttggg ttttcacctg   1200

tacactggct atggaagcac ggaagccggt ggtgtcttgt tggatactgt tgttcagcgt   1260

cctcccgtca cagactacaa actggtcgat gtgcctgagc tgggatatta tgctaccgat   1320

ctgccgcatc ctcgtggaga gttgcttttg aaatcccaca ccttgattcc tggttattac   1380

cgtcgtcccg acctgaccgc cgccatcttt gacgccgacg gttactaccg taccggcgat   1440

gtttttgctg aaaccggtcc ggatcgtctt gtctatgttg accgtactaa agacacgttg   1500

aagctgtctc agggtgagtt cgttgccgtg tcccgtttgg aaacagtctt gttggactct   1560

cctcttgttc aacacttgta cctttatggc aactctgagc gtgcatattt gcttgcggtc   1620

gtcgttccta cgccagatgc gttggctggt tgtggaggcg acacggaagc cctgcgtccg   1680

ttgctgatgg agtccctgcg ttctgtcgca cgtcgtgccg gtttgaacgc ttacgaaatc   1740

cctcgtggta tcttggtcga accggaacct tttagcccgg agaacggtct gttcaccgag   1800

tctcataagt tgctgcgtcc acgtcttaaa gaacgttatg gtcctgcttt ggagttgctg   1860

tacgatcgtc ttgccgacgg tcaggatcgt cgtttgcgtg agcttcgtcg tactggtgcc   1920

gaccgtcctg tccaggagac cgtgctgcgt gccgctcaag ccttgttggg ctccccaggc   1980

tctgacttgc gtcccggcgc tcactttacg gatcttggtg gcgactcttt gtccgcagtc   2040

agcttttccg agttgatgaa ggaaattttt catgtcgatg ttcctgttgg cgccattatt   2100

ggtccagccg ctgacctggc cgaagttgcg cgttacatca ctgctgctcg tcgtcctgcg   2160

ggagcgcccc gtccaacgcc agcctccgtt catggcgaac atcgtactga ggtccgtgcc   2220

ggtgatctgg ccccagagaa gtttttggat gcgccgactc tggcagcagc ccccgctttg   2280

ccacgtccag acggtgacgt tcgtactgtg ttgctgaccg gtgccactgg ctacttgggt   2340

cgttttctgt gtttggaatg gctggagcgt ttggcgccaa gcggtggtcg tcttgtttgt   2400

ttggttcgtg gtagcgacgc aacggtcgcg gcccgtcgtc tggaggcggc gtttgactct   2460

ggcgacacgg cacttttgcg tcgttatcgt aaggccgcag gaaaaacgtt ggatgttgtc   2520

gccggtgaca ttggcgagcc tttgctgggt ctggccgagg agacctggcg tgagcttgct   2580

ggtgccgtcg atttgattgt gcaccctgcc gctcttgtca accacttgtt gccctacggt   2640

gagctgttcg gtccaaatgt cgtgggcacc gctgaggcga tccgtctggc cctgaccacc   2700

cgtttgaagc ctgttaatca cgtttccacc gtggcggtgt gcctgggtac gcccgccgag   2760

accgccgacg aaaacgctga tattcgtgcc gctgttccgg tgcgtacaac aggtcaaggc   2820

tatgccgacg gttacgcgac ctctaaatgg gctggcgagg tccttcttcg tgaggcacat   2880

gaacgttacg gtttgccagt ggctgtcttt cgttctgaca tggttttggc acaccgtact   2940

tacactggcc aagttaacgt cccagatgtt ttgacacgtt tgttgcttag ccttgtggcg   3000

actggcatcg ccccaggttc tttttaccgt accgatacac gtgcccacta tgacggcctg   3060

ccagtggact ttaccgccga ggctgttgtg gcactgggag cccccattac tgagggtcac   3120

cgtacgttca acgttctgaa cccccacgat gacggtgtga gccttgatac ttttgtggat   3180

tggttgatcg aggcaggtca tcctattcgt cgtatcgacg atcatggtgc ttggttgact   3240

cgtttcaccg ccgccttgcg tgcgctgcct gagaagcaac gtcaacactc cttgttgccg   3300

cttatcggtg cctgggcgga gcccggcgaa ggtgcccccg gtccccttct gccagcacgt   3360

cgttttcatg cagcggtccg tgcagccggt gtgggtcctg aacgtgatat tccgcgtgtc   3420

tcccctgatt tgattcgtaa gtacgtgacc gatttgcgtg ccctgggttt gttggcaggt   3480

ccgtaatgag gccaaactgg ccaccatcac catcacc                            3517


<210>  6
<211>  1148
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence from optimized codon sequence coding 
       for Streptomyces griseus CAR peptide.

<400>  6

Met Ala Glu Pro Leu Asp Ala Ala Thr Ala Ser Ala His Asp Pro Gly 
1               5                   10                  15      


Gln Gly Leu Ala Glu Ala Leu Ala Ala Val Glu Pro Gly Arg Ala Leu 
            20                  25                  30          


Ala Glu Val Met Ala Ser Val Leu Glu Gly His Gly Asp Arg Pro Ala 
        35                  40                  45              


Leu Gly Glu Arg Ala Arg Glu Pro Glu Thr Gly Arg Leu Leu Pro His 
    50                  55                  60                  


Phe Asp Thr Ile Ser Tyr Arg Glu Leu Trp Ser Arg Val Arg Ala Leu 
65                  70                  75                  80  


Ala Gly Arg Trp His His Asp Pro Glu Tyr Pro Leu Gly Pro Gly Asp 
                85                  90                  95      


Arg Ile Cys Thr Leu Gly Phe Thr Ser Thr Asp Tyr Ala Thr Leu Asp 
            100                 105                 110         


Leu Ala Cys Ile His Leu Gly Ala Val Pro Val Pro Leu Pro Ser Asn 
        115                 120                 125             


Ala Pro Leu Pro Arg Leu Ala Pro Val Val Glu Glu Ser Gly Pro Thr 
    130                 135                 140                 


Val Leu Ala Ala Ser Val Asp Arg Leu Asp Thr Ala Ile Asp Val Val 
145                 150                 155                 160 


Leu Ala Ser Ser Thr Ile Arg Arg Leu Leu Val Phe Asp Asp Gly Pro 
                165                 170                 175     


Gly Ala Thr Arg Pro Gly Gly Ala Leu Ala Ala Ala Arg Gln Arg Leu 
            180                 185                 190         


Ser Gly Ser Pro Val Thr Val Asp Thr Leu Ala Gly Leu Ile Asp Arg 
        195                 200                 205             


Gly Arg Asp Leu Pro Pro Pro Pro Leu Tyr Ile Pro Asp Pro Gly Glu 
    210                 215                 220                 


Asp Pro Leu Ala Leu Leu Ile Tyr Thr Ser Gly Ser Thr Gly Ala Pro 
225                 230                 235                 240 


Lys Gly Ala Met Tyr Thr Gln Arg Leu Leu Gly Thr Ala Trp Tyr Gly 
                245                 250                 255     


Phe Ser Tyr Gly Ala Ala Asp Thr Pro Ala Ile Ser Val Leu Tyr Leu 
            260                 265                 270         


Pro Gln Ser His Leu Ala Gly Arg Tyr Ala Val Met Gly Ser Leu Val 
        275                 280                 285             


Lys Gly Gly Thr Gly Tyr Phe Thr Ala Ala Asp Asp Leu Ser Thr Leu 
    290                 295                 300                 


Phe Glu Asp Ile Ala Leu Val Arg Pro Thr Glu Leu Thr Met Val Pro 
305                 310                 315                 320 


Arg Leu Cys Asp Met Leu Leu Gln His Tyr Arg Ser Glu Arg Asp Arg 
                325                 330                 335     


Arg Ala Asp Glu Pro Gly Asp Ile Glu Ala Ala Val Thr Lys Ala Val 
            340                 345                 350         


Arg Glu Asp Phe Leu Gly Gly Arg Val Ala Lys Ala Phe Val Gly Thr 
        355                 360                 365             


Ala Pro Leu Ser Ala Glu Leu Thr Ala Phe Val Glu Ser Val Leu Gly 
    370                 375                 380                 


Phe His Leu Tyr Thr Gly Tyr Gly Ser Thr Glu Ala Gly Gly Val Leu 
385                 390                 395                 400 


Leu Asp Thr Val Val Gln Arg Pro Pro Val Thr Asp Tyr Lys Leu Val 
                405                 410                 415     


Asp Val Pro Glu Leu Gly Tyr Tyr Ala Thr Asp Leu Pro His Pro Arg 
            420                 425                 430         


Gly Glu Leu Leu Leu Lys Ser His Thr Leu Ile Pro Gly Tyr Tyr Arg 
        435                 440                 445             


Arg Pro Asp Leu Thr Ala Ala Ile Phe Asp Ala Asp Gly Tyr Tyr Arg 
    450                 455                 460                 


Thr Gly Asp Val Phe Ala Glu Thr Gly Pro Asp Arg Leu Val Tyr Val 
465                 470                 475                 480 


Asp Arg Thr Lys Asp Thr Leu Lys Leu Ser Gln Gly Glu Phe Val Ala 
                485                 490                 495     


Val Ser Arg Leu Glu Thr Val Leu Leu Asp Ser Pro Leu Val Gln His 
            500                 505                 510         


Leu Tyr Leu Tyr Gly Asn Ser Glu Arg Ala Tyr Leu Leu Ala Val Val 
        515                 520                 525             


Val Pro Thr Pro Asp Ala Leu Ala Gly Cys Gly Gly Asp Thr Glu Ala 
    530                 535                 540                 


Leu Arg Pro Leu Leu Met Glu Ser Leu Arg Ser Val Ala Arg Arg Ala 
545                 550                 555                 560 


Gly Leu Asn Ala Tyr Glu Ile Pro Arg Gly Ile Leu Val Glu Pro Glu 
                565                 570                 575     


Pro Phe Ser Pro Glu Asn Gly Leu Phe Thr Glu Ser His Lys Leu Leu 
            580                 585                 590         


Arg Pro Arg Leu Lys Glu Arg Tyr Gly Pro Ala Leu Glu Leu Leu Tyr 
        595                 600                 605             


Asp Arg Leu Ala Asp Gly Gln Asp Arg Arg Leu Arg Glu Leu Arg Arg 
    610                 615                 620                 


Thr Gly Ala Asp Arg Pro Val Gln Glu Thr Val Leu Arg Ala Ala Gln 
625                 630                 635                 640 


Ala Leu Leu Gly Ser Pro Gly Ser Asp Leu Arg Pro Gly Ala His Phe 
                645                 650                 655     


Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala Val Ser Phe Ser Glu Leu 
            660                 665                 670         


Met Lys Glu Ile Phe His Val Asp Val Pro Val Gly Ala Ile Ile Gly 
        675                 680                 685             


Pro Ala Ala Asp Leu Ala Glu Val Ala Arg Tyr Ile Thr Ala Ala Arg 
    690                 695                 700                 


Arg Pro Ala Gly Ala Pro Arg Pro Thr Pro Ala Ser Val His Gly Glu 
705                 710                 715                 720 


His Arg Thr Glu Val Arg Ala Gly Asp Leu Ala Pro Glu Lys Phe Leu 
                725                 730                 735     


Asp Ala Pro Thr Leu Ala Ala Ala Pro Ala Leu Pro Arg Pro Asp Gly 
            740                 745                 750         


Asp Val Arg Thr Val Leu Leu Thr Gly Ala Thr Gly Tyr Leu Gly Arg 
        755                 760                 765             


Phe Leu Cys Leu Glu Trp Leu Glu Arg Leu Ala Pro Ser Gly Gly Arg 
    770                 775                 780                 


Leu Val Cys Leu Val Arg Gly Ser Asp Ala Thr Val Ala Ala Arg Arg 
785                 790                 795                 800 


Leu Glu Ala Ala Phe Asp Ser Gly Asp Thr Ala Leu Leu Arg Arg Tyr 
                805                 810                 815     


Arg Lys Ala Ala Gly Lys Thr Leu Asp Val Val Ala Gly Asp Ile Gly 
            820                 825                 830         


Glu Pro Leu Leu Gly Leu Ala Glu Glu Thr Trp Arg Glu Leu Ala Gly 
        835                 840                 845             


Ala Val Asp Leu Ile Val His Pro Ala Ala Leu Val Asn His Leu Leu 
    850                 855                 860                 


Pro Tyr Gly Glu Leu Phe Gly Pro Asn Val Val Gly Thr Ala Glu Ala 
865                 870                 875                 880 


Ile Arg Leu Ala Leu Thr Thr Arg Leu Lys Pro Val Asn His Val Ser 
                885                 890                 895     


Thr Val Ala Val Cys Leu Gly Thr Pro Ala Glu Thr Ala Asp Glu Asn 
            900                 905                 910         


Ala Asp Ile Arg Ala Ala Val Pro Val Arg Thr Thr Gly Gln Gly Tyr 
        915                 920                 925             


Ala Asp Gly Tyr Ala Thr Ser Lys Trp Ala Gly Glu Val Leu Leu Arg 
    930                 935                 940                 


Glu Ala His Glu Arg Tyr Gly Leu Pro Val Ala Val Phe Arg Ser Asp 
945                 950                 955                 960 


Met Val Leu Ala His Arg Thr Tyr Thr Gly Gln Val Asn Val Pro Asp 
                965                 970                 975     


Val Leu Thr Arg Leu Leu Leu Ser Leu Val Ala Thr Gly Ile Ala Pro 
            980                 985                 990         


Gly Ser Phe Tyr Arg Thr Asp Thr  Arg Ala His Tyr Asp  Gly Leu Pro 
        995                 1000                 1005             


Val Asp  Phe Thr Ala Glu Ala  Val Val Ala Leu Gly  Ala Pro Ile 
    1010                 1015                 1020             


Thr Glu  Gly His Arg Thr Phe  Asn Val Leu Asn Pro  His Asp Asp 
    1025                 1030                 1035             


Gly Val  Ser Leu Asp Thr Phe  Val Asp Trp Leu Ile  Glu Ala Gly 
    1040                 1045                 1050             


His Pro  Ile Arg Arg Ile Asp  Asp His Gly Ala Trp  Leu Thr Arg 
    1055                 1060                 1065             


Phe Thr  Ala Ala Leu Arg Ala  Leu Pro Glu Lys Gln  Arg Gln His 
    1070                 1075                 1080             


Ser Leu  Leu Pro Leu Ile Gly  Ala Trp Ala Glu Pro  Gly Glu Gly 
    1085                 1090                 1095             


Ala Pro  Gly Pro Leu Leu Pro  Ala Arg Arg Phe His  Ala Ala Val 
    1100                 1105                 1110             


Arg Ala  Ala Gly Val Gly Pro  Glu Arg Asp Ile Pro  Arg Val Ser 
    1115                 1120                 1125             


Pro Asp  Leu Ile Arg Lys Tyr  Val Thr Asp Leu Arg  Ala Leu Gly 
    1130                 1135                 1140             


Leu Leu  Ala Gly Pro 
    1145             


<210>  7
<211>  739
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence including optimized codon sequence coding 
       for Nocardia NRRL5646 PPTase peptide.

<400>  7
acaatctaga ggccagcctg gccataagga gatatacata tgatcgaaac tattttgccc     60

gcaggtgttg aatctgccga gcttttggaa tatcctgagg accttaaagc ccaccctgct    120

gaagaacatc ttattgccaa atccgtcgaa aaacgtcgtc gtgatttcat tggtgcccgt    180

cattgcgcgc gtctggccct ggccgagttg ggcgagccac ccgtcgcaat tggcaaaggt    240

gaacgtggtg cccctatttg gccgcgtggc gttgtcggct ctcttaccca ctgcgacggc    300

taccgtgccg ccgcagtcgc ccataagatg cgtttccgtt ctattggcat tgacgccgaa    360

ccgcacgcca cccttcctga aggagtcctg gactctgttt ctcttccacc tgaacgtgag    420

tggttgaaga ccactgattc tgctttgcat cttgatcgtt tgttgttctg cgcgaaggaa    480

gcaacttata aggcttggtg gccattgacc gctcgttggc ttggctttga ggaagcacat    540

attacttttg agattgagga tggtagcgcc gatagcggca atggtacttt tcatagcgaa    600

ctgttggttc ctggtcagac gaatgacggt ggtactcctc ttcttagctt tgatggacgt    660

tggctgattg ccgatggttt tatcttgacc gcaattgcgt atgcgtaatg aggccaaact    720

ggccaccatc accatcacc                                                 739


<210>  8
<211>  222
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence from optimized codon sequence coding 
       for Nocardia NRRL5646 PPTase peptide.

<400>  8

Met Ile Glu Thr Ile Leu Pro Ala Gly Val Glu Ser Ala Glu Leu Leu 
1               5                   10                  15      


Glu Tyr Pro Glu Asp Leu Lys Ala His Pro Ala Glu Glu His Leu Ile 
            20                  25                  30          


Ala Lys Ser Val Glu Lys Arg Arg Arg Asp Phe Ile Gly Ala Arg His 
        35                  40                  45              


Cys Ala Arg Leu Ala Leu Ala Glu Leu Gly Glu Pro Pro Val Ala Ile 
    50                  55                  60                  


Gly Lys Gly Glu Arg Gly Ala Pro Ile Trp Pro Arg Gly Val Val Gly 
65                  70                  75                  80  


Ser Leu Thr His Cys Asp Gly Tyr Arg Ala Ala Ala Val Ala His Lys 
                85                  90                  95      


Met Arg Phe Arg Ser Ile Gly Ile Asp Ala Glu Pro His Ala Thr Leu 
            100                 105                 110         


Pro Glu Gly Val Leu Asp Ser Val Ser Leu Pro Pro Glu Arg Glu Trp 
        115                 120                 125             


Leu Lys Thr Thr Asp Ser Ala Leu His Leu Asp Arg Leu Leu Phe Cys 
    130                 135                 140                 


Ala Lys Glu Ala Thr Tyr Lys Ala Trp Trp Pro Leu Thr Ala Arg Trp 
145                 150                 155                 160 


Leu Gly Phe Glu Glu Ala His Ile Thr Phe Glu Ile Glu Asp Gly Ser 
                165                 170                 175     


Ala Asp Ser Gly Asn Gly Thr Phe His Ser Glu Leu Leu Val Pro Gly 
            180                 185                 190         


Gln Thr Asn Asp Gly Gly Thr Pro Leu Leu Ser Phe Asp Gly Arg Trp 
        195                 200                 205             


Leu Ile Ala Asp Gly Phe Ile Leu Thr Ala Ile Ala Tyr Ala 
    210                 215                 220         


<210>  9
<211>  1178
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide having GDIH at N-terminal of CAR peptide from 
       Nocardia NRRL5646.

<400>  9

Gly Asp Ile His Met Ala Val Asp Ser Pro Asp Glu Arg Leu Gln Arg 
1               5                   10                  15      


Arg Ile Ala Gln Leu Phe Ala Glu Asp Glu Gln Val Lys Ala Ala Arg 
            20                  25                  30          


Pro Leu Glu Ala Val Ser Ala Ala Val Ser Ala Pro Gly Met Arg Leu 
        35                  40                  45              


Ala Gln Ile Ala Ala Thr Val Met Ala Gly Tyr Ala Asp Arg Pro Ala 
    50                  55                  60                  


Ala Gly Gln Arg Ala Phe Glu Leu Asn Thr Asp Asp Ala Thr Gly Arg 
65                  70                  75                  80  


Thr Ser Leu Arg Leu Leu Pro Arg Phe Glu Thr Ile Thr Tyr Arg Glu 
                85                  90                  95      


Leu Trp Gln Arg Val Gly Glu Val Ala Ala Ala Trp His His Asp Pro 
            100                 105                 110         


Glu Asn Pro Leu Arg Ala Gly Asp Phe Val Ala Leu Leu Gly Phe Thr 
        115                 120                 125             


Ser Ile Asp Tyr Ala Thr Leu Asp Leu Ala Asp Ile His Leu Gly Ala 
    130                 135                 140                 


Val Thr Val Pro Leu Gln Ala Ser Ala Ala Val Ser Gln Leu Ile Ala 
145                 150                 155                 160 


Ile Leu Thr Glu Thr Ser Pro Arg Leu Leu Ala Ser Thr Pro Glu His 
                165                 170                 175     


Leu Asp Ala Ala Val Glu Cys Leu Leu Ala Gly Thr Thr Pro Glu Arg 
            180                 185                 190         


Leu Val Val Phe Asp Tyr His Pro Glu Asp Asp Asp Gln Arg Ala Ala 
        195                 200                 205             


Phe Glu Ser Ala Arg Arg Arg Leu Ala Asp Ala Gly Ser Leu Val Ile 
    210                 215                 220                 


Val Glu Thr Leu Asp Ala Val Arg Ala Arg Gly Arg Asp Leu Pro Ala 
225                 230                 235                 240 


Ala Pro Leu Phe Val Pro Asp Thr Asp Asp Asp Pro Leu Ala Leu Leu 
                245                 250                 255     


Ile Tyr Thr Ser Gly Ser Thr Gly Thr Pro Lys Gly Ala Met Tyr Thr 
            260                 265                 270         


Asn Arg Leu Ala Ala Thr Met Trp Gln Gly Asn Ser Met Leu Gln Gly 
        275                 280                 285             


Asn Ser Gln Arg Val Gly Ile Asn Leu Asn Tyr Met Pro Met Ser His 
    290                 295                 300                 


Ile Ala Gly Arg Ile Ser Leu Phe Gly Val Leu Ala Arg Gly Gly Thr 
305                 310                 315                 320 


Ala Tyr Phe Ala Ala Lys Ser Asp Met Ser Thr Leu Phe Glu Asp Ile 
                325                 330                 335     


Gly Leu Val Arg Pro Thr Glu Ile Phe Phe Val Pro Arg Val Cys Asp 
            340                 345                 350         


Met Val Phe Gln Arg Tyr Gln Ser Glu Leu Asp Arg Arg Ser Val Ala 
        355                 360                 365             


Gly Ala Asp Leu Asp Thr Leu Asp Arg Glu Val Lys Ala Asp Leu Arg 
    370                 375                 380                 


Gln Asn Tyr Leu Gly Gly Arg Phe Leu Val Ala Val Val Gly Ser Ala 
385                 390                 395                 400 


Pro Leu Ala Ala Glu Met Lys Thr Phe Met Glu Ser Val Leu Asp Leu 
                405                 410                 415     


Pro Leu His Asp Gly Tyr Gly Ser Thr Glu Ala Gly Ala Ser Val Leu 
            420                 425                 430         


Leu Asp Asn Gln Ile Gln Arg Pro Pro Val Leu Asp Tyr Lys Leu Val 
        435                 440                 445             


Asp Val Pro Glu Leu Gly Tyr Phe Arg Thr Asp Arg Pro His Pro Arg 
    450                 455                 460                 


Gly Glu Leu Leu Leu Lys Ala Glu Thr Thr Ile Pro Gly Tyr Tyr Lys 
465                 470                 475                 480 


Arg Pro Glu Val Thr Ala Glu Ile Phe Asp Glu Asp Gly Phe Tyr Lys 
                485                 490                 495     


Thr Gly Asp Ile Val Ala Glu Leu Glu His Asp Arg Leu Val Tyr Val 
            500                 505                 510         


Asp Arg Arg Asn Asn Val Leu Lys Leu Ser Gln Gly Glu Phe Val Thr 
        515                 520                 525             


Val Ala His Leu Glu Ala Val Phe Ala Ser Ser Pro Leu Ile Arg Gln 
    530                 535                 540                 


Ile Phe Ile Tyr Gly Ser Ser Glu Arg Ser Tyr Leu Leu Ala Val Ile 
545                 550                 555                 560 


Val Pro Thr Asp Asp Ala Leu Arg Gly Arg Asp Thr Ala Thr Leu Lys 
                565                 570                 575     


Ser Ala Leu Ala Glu Ser Ile Gln Arg Ile Ala Lys Asp Ala Asn Leu 
            580                 585                 590         


Gln Pro Tyr Glu Ile Pro Arg Asp Phe Leu Ile Glu Thr Glu Pro Phe 
        595                 600                 605             


Thr Ile Ala Asn Gly Leu Leu Ser Gly Ile Ala Lys Leu Leu Arg Pro 
    610                 615                 620                 


Asn Leu Lys Glu Arg Tyr Gly Ala Gln Leu Glu Gln Met Tyr Thr Asp 
625                 630                 635                 640 


Leu Ala Thr Gly Gln Ala Asp Glu Leu Leu Ala Leu Arg Arg Glu Ala 
                645                 650                 655     


Ala Asp Leu Pro Val Leu Glu Thr Val Ser Arg Ala Ala Lys Ala Met 
            660                 665                 670         


Leu Gly Val Ala Ser Ala Asp Met Arg Pro Asp Ala His Phe Thr Asp 
        675                 680                 685             


Leu Gly Gly Asp Ser Leu Ser Ala Leu Ser Phe Ser Asn Leu Leu His 
    690                 695                 700                 


Glu Ile Phe Gly Val Glu Val Pro Val Gly Val Val Val Ser Pro Ala 
705                 710                 715                 720 


Asn Glu Leu Arg Asp Leu Ala Asn Tyr Ile Glu Ala Glu Arg Asn Ser 
                725                 730                 735     


Gly Ala Lys Arg Pro Thr Phe Thr Ser Val His Gly Gly Gly Ser Glu 
            740                 745                 750         


Ile Arg Ala Ala Asp Leu Thr Leu Asp Lys Phe Ile Asp Ala Arg Thr 
        755                 760                 765             


Leu Ala Ala Ala Asp Ser Ile Pro His Ala Pro Val Pro Ala Gln Thr 
    770                 775                 780                 


Val Leu Leu Thr Gly Ala Asn Gly Tyr Leu Gly Arg Phe Leu Cys Leu 
785                 790                 795                 800 


Glu Trp Leu Glu Arg Leu Asp Lys Thr Gly Gly Thr Leu Ile Cys Val 
                805                 810                 815     


Val Arg Gly Ser Asp Ala Ala Ala Ala Arg Lys Arg Leu Asp Ser Ala 
            820                 825                 830         


Phe Asp Ser Gly Asp Pro Gly Leu Leu Glu His Tyr Gln Gln Leu Ala 
        835                 840                 845             


Ala Arg Thr Leu Glu Val Leu Ala Gly Asp Ile Gly Asp Pro Asn Leu 
    850                 855                 860                 


Gly Leu Asp Asp Ala Thr Trp Gln Arg Leu Ala Glu Thr Val Asp Leu 
865                 870                 875                 880 


Ile Val His Pro Ala Ala Leu Val Asn His Val Leu Pro Tyr Thr Gln 
                885                 890                 895     


Leu Phe Gly Pro Asn Val Val Gly Thr Ala Glu Ile Val Arg Leu Ala 
            900                 905                 910         


Ile Thr Ala Arg Arg Lys Pro Val Thr Tyr Leu Ser Thr Val Gly Val 
        915                 920                 925             


Ala Asp Gln Val Asp Pro Ala Glu Tyr Gln Glu Asp Ser Asp Val Arg 
    930                 935                 940                 


Glu Met Ser Ala Val Arg Val Val Arg Glu Ser Tyr Ala Asn Gly Tyr 
945                 950                 955                 960 


Gly Asn Ser Lys Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp 
                965                 970                 975     


Leu Cys Gly Leu Pro Val Ala Val Phe Arg Ser Asp Met Ile Leu Ala 
            980                 985                 990         


His Ser Arg Tyr Ala Gly Gln Leu  Asn Val Gln Asp Val  Phe Thr Arg 
        995                 1000                 1005             


Leu Ile  Leu Ser Leu Val Ala  Thr Gly Ile Ala Pro  Tyr Ser Phe 
    1010                 1015                 1020             


Tyr Arg  Thr Asp Ala Asp Gly  Asn Arg Gln Arg Ala  His Tyr Asp 
    1025                 1030                 1035             


Gly Leu  Pro Ala Asp Phe Thr  Ala Ala Ala Ile Thr  Ala Leu Gly 
    1040                 1045                 1050             


Ile Gln  Ala Thr Glu Gly Phe  Arg Thr Tyr Asp Val  Leu Asn Pro 
    1055                 1060                 1065             


Tyr Asp  Asp Gly Ile Ser Leu  Asp Glu Phe Val Asp  Trp Leu Val 
    1070                 1075                 1080             


Glu Ser  Gly His Pro Ile Gln  Arg Ile Thr Asp Tyr  Ser Asp Trp 
    1085                 1090                 1095             


Phe His  Arg Phe Glu Thr Ala  Ile Arg Ala Leu Pro  Glu Lys Gln 
    1100                 1105                 1110             


Arg Gln  Ala Ser Val Leu Pro  Leu Leu Asp Ala Tyr  Arg Asn Pro 
    1115                 1120                 1125             


Cys Pro  Ala Val Arg Gly Ala  Ile Leu Pro Ala Lys  Glu Phe Gln 
    1130                 1135                 1140             


Ala Ala  Val Gln Thr Ala Lys  Ile Gly Pro Glu Gln  Asp Ile Pro 
    1145                 1150                 1155             


His Leu  Ser Ala Pro Leu Ile  Asp Lys Tyr Val Ser  Asp Leu Glu 
    1160                 1165                 1170             


Leu Leu  Gln Leu Leu 
    1175             


<210>  10
<211>  1152
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide having GDIH at N-terminal of CAR peptide from 
       Streptomyces griseus.

<400>  10

Gly Asp Ile His Met Ala Glu Pro Leu Asp Ala Ala Thr Ala Ser Ala 
1               5                   10                  15      


His Asp Pro Gly Gln Gly Leu Ala Glu Ala Leu Ala Ala Val Glu Pro 
            20                  25                  30          


Gly Arg Ala Leu Ala Glu Val Met Ala Ser Val Leu Glu Gly His Gly 
        35                  40                  45              


Asp Arg Pro Ala Leu Gly Glu Arg Ala Arg Glu Pro Glu Thr Gly Arg 
    50                  55                  60                  


Leu Leu Pro His Phe Asp Thr Ile Ser Tyr Arg Glu Leu Trp Ser Arg 
65                  70                  75                  80  


Val Arg Ala Leu Ala Gly Arg Trp His His Asp Pro Glu Tyr Pro Leu 
                85                  90                  95      


Gly Pro Gly Asp Arg Ile Cys Thr Leu Gly Phe Thr Ser Thr Asp Tyr 
            100                 105                 110         


Ala Thr Leu Asp Leu Ala Cys Ile His Leu Gly Ala Val Pro Val Pro 
        115                 120                 125             


Leu Pro Ser Asn Ala Pro Leu Pro Arg Leu Ala Pro Val Val Glu Glu 
    130                 135                 140                 


Ser Gly Pro Thr Val Leu Ala Ala Ser Val Asp Arg Leu Asp Thr Ala 
145                 150                 155                 160 


Ile Asp Val Val Leu Ala Ser Ser Thr Ile Arg Arg Leu Leu Val Phe 
                165                 170                 175     


Asp Asp Gly Pro Gly Ala Thr Arg Pro Gly Gly Ala Leu Ala Ala Ala 
            180                 185                 190         


Arg Gln Arg Leu Ser Gly Ser Pro Val Thr Val Asp Thr Leu Ala Gly 
        195                 200                 205             


Leu Ile Asp Arg Gly Arg Asp Leu Pro Pro Pro Pro Leu Tyr Ile Pro 
    210                 215                 220                 


Asp Pro Gly Glu Asp Pro Leu Ala Leu Leu Ile Tyr Thr Ser Gly Ser 
225                 230                 235                 240 


Thr Gly Ala Pro Lys Gly Ala Met Tyr Thr Gln Arg Leu Leu Gly Thr 
                245                 250                 255     


Ala Trp Tyr Gly Phe Ser Tyr Gly Ala Ala Asp Thr Pro Ala Ile Ser 
            260                 265                 270         


Val Leu Tyr Leu Pro Gln Ser His Leu Ala Gly Arg Tyr Ala Val Met 
        275                 280                 285             


Gly Ser Leu Val Lys Gly Gly Thr Gly Tyr Phe Thr Ala Ala Asp Asp 
    290                 295                 300                 


Leu Ser Thr Leu Phe Glu Asp Ile Ala Leu Val Arg Pro Thr Glu Leu 
305                 310                 315                 320 


Thr Met Val Pro Arg Leu Cys Asp Met Leu Leu Gln His Tyr Arg Ser 
                325                 330                 335     


Glu Arg Asp Arg Arg Ala Asp Glu Pro Gly Asp Ile Glu Ala Ala Val 
            340                 345                 350         


Thr Lys Ala Val Arg Glu Asp Phe Leu Gly Gly Arg Val Ala Lys Ala 
        355                 360                 365             


Phe Val Gly Thr Ala Pro Leu Ser Ala Glu Leu Thr Ala Phe Val Glu 
    370                 375                 380                 


Ser Val Leu Gly Phe His Leu Tyr Thr Gly Tyr Gly Ser Thr Glu Ala 
385                 390                 395                 400 


Gly Gly Val Leu Leu Asp Thr Val Val Gln Arg Pro Pro Val Thr Asp 
                405                 410                 415     


Tyr Lys Leu Val Asp Val Pro Glu Leu Gly Tyr Tyr Ala Thr Asp Leu 
            420                 425                 430         


Pro His Pro Arg Gly Glu Leu Leu Leu Lys Ser His Thr Leu Ile Pro 
        435                 440                 445             


Gly Tyr Tyr Arg Arg Pro Asp Leu Thr Ala Ala Ile Phe Asp Ala Asp 
    450                 455                 460                 


Gly Tyr Tyr Arg Thr Gly Asp Val Phe Ala Glu Thr Gly Pro Asp Arg 
465                 470                 475                 480 


Leu Val Tyr Val Asp Arg Thr Lys Asp Thr Leu Lys Leu Ser Gln Gly 
                485                 490                 495     


Glu Phe Val Ala Val Ser Arg Leu Glu Thr Val Leu Leu Asp Ser Pro 
            500                 505                 510         


Leu Val Gln His Leu Tyr Leu Tyr Gly Asn Ser Glu Arg Ala Tyr Leu 
        515                 520                 525             


Leu Ala Val Val Val Pro Thr Pro Asp Ala Leu Ala Gly Cys Gly Gly 
    530                 535                 540                 


Asp Thr Glu Ala Leu Arg Pro Leu Leu Met Glu Ser Leu Arg Ser Val 
545                 550                 555                 560 


Ala Arg Arg Ala Gly Leu Asn Ala Tyr Glu Ile Pro Arg Gly Ile Leu 
                565                 570                 575     


Val Glu Pro Glu Pro Phe Ser Pro Glu Asn Gly Leu Phe Thr Glu Ser 
            580                 585                 590         


His Lys Leu Leu Arg Pro Arg Leu Lys Glu Arg Tyr Gly Pro Ala Leu 
        595                 600                 605             


Glu Leu Leu Tyr Asp Arg Leu Ala Asp Gly Gln Asp Arg Arg Leu Arg 
    610                 615                 620                 


Glu Leu Arg Arg Thr Gly Ala Asp Arg Pro Val Gln Glu Thr Val Leu 
625                 630                 635                 640 


Arg Ala Ala Gln Ala Leu Leu Gly Ser Pro Gly Ser Asp Leu Arg Pro 
                645                 650                 655     


Gly Ala His Phe Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala Val Ser 
            660                 665                 670         


Phe Ser Glu Leu Met Lys Glu Ile Phe His Val Asp Val Pro Val Gly 
        675                 680                 685             


Ala Ile Ile Gly Pro Ala Ala Asp Leu Ala Glu Val Ala Arg Tyr Ile 
    690                 695                 700                 


Thr Ala Ala Arg Arg Pro Ala Gly Ala Pro Arg Pro Thr Pro Ala Ser 
705                 710                 715                 720 


Val His Gly Glu His Arg Thr Glu Val Arg Ala Gly Asp Leu Ala Pro 
                725                 730                 735     


Glu Lys Phe Leu Asp Ala Pro Thr Leu Ala Ala Ala Pro Ala Leu Pro 
            740                 745                 750         


Arg Pro Asp Gly Asp Val Arg Thr Val Leu Leu Thr Gly Ala Thr Gly 
        755                 760                 765             


Tyr Leu Gly Arg Phe Leu Cys Leu Glu Trp Leu Glu Arg Leu Ala Pro 
    770                 775                 780                 


Ser Gly Gly Arg Leu Val Cys Leu Val Arg Gly Ser Asp Ala Thr Val 
785                 790                 795                 800 


Ala Ala Arg Arg Leu Glu Ala Ala Phe Asp Ser Gly Asp Thr Ala Leu 
                805                 810                 815     


Leu Arg Arg Tyr Arg Lys Ala Ala Gly Lys Thr Leu Asp Val Val Ala 
            820                 825                 830         


Gly Asp Ile Gly Glu Pro Leu Leu Gly Leu Ala Glu Glu Thr Trp Arg 
        835                 840                 845             


Glu Leu Ala Gly Ala Val Asp Leu Ile Val His Pro Ala Ala Leu Val 
    850                 855                 860                 


Asn His Leu Leu Pro Tyr Gly Glu Leu Phe Gly Pro Asn Val Val Gly 
865                 870                 875                 880 


Thr Ala Glu Ala Ile Arg Leu Ala Leu Thr Thr Arg Leu Lys Pro Val 
                885                 890                 895     


Asn His Val Ser Thr Val Ala Val Cys Leu Gly Thr Pro Ala Glu Thr 
            900                 905                 910         


Ala Asp Glu Asn Ala Asp Ile Arg Ala Ala Val Pro Val Arg Thr Thr 
        915                 920                 925             


Gly Gln Gly Tyr Ala Asp Gly Tyr Ala Thr Ser Lys Trp Ala Gly Glu 
    930                 935                 940                 


Val Leu Leu Arg Glu Ala His Glu Arg Tyr Gly Leu Pro Val Ala Val 
945                 950                 955                 960 


Phe Arg Ser Asp Met Val Leu Ala His Arg Thr Tyr Thr Gly Gln Val 
                965                 970                 975     


Asn Val Pro Asp Val Leu Thr Arg Leu Leu Leu Ser Leu Val Ala Thr 
            980                 985                 990         


Gly Ile Ala Pro Gly Ser Phe Tyr  Arg Thr Asp Thr Arg  Ala His Tyr 
        995                 1000                 1005             


Asp Gly  Leu Pro Val Asp Phe  Thr Ala Glu Ala Val  Val Ala Leu 
    1010                 1015                 1020             


Gly Ala  Pro Ile Thr Glu Gly  His Arg Thr Phe Asn  Val Leu Asn 
    1025                 1030                 1035             


Pro His  Asp Asp Gly Val Ser  Leu Asp Thr Phe Val  Asp Trp Leu 
    1040                 1045                 1050             


Ile Glu  Ala Gly His Pro Ile  Arg Arg Ile Asp Asp  His Gly Ala 
    1055                 1060                 1065             


Trp Leu  Thr Arg Phe Thr Ala  Ala Leu Arg Ala Leu  Pro Glu Lys 
    1070                 1075                 1080             


Gln Arg  Gln His Ser Leu Leu  Pro Leu Ile Gly Ala  Trp Ala Glu 
    1085                 1090                 1095             


Pro Gly  Glu Gly Ala Pro Gly  Pro Leu Leu Pro Ala  Arg Arg Phe 
    1100                 1105                 1110             


His Ala  Ala Val Arg Ala Ala  Gly Val Gly Pro Glu  Arg Asp Ile 
    1115                 1120                 1125             


Pro Arg  Val Ser Pro Asp Leu  Ile Arg Lys Tyr Val  Thr Asp Leu 
    1130                 1135                 1140             


Arg Ala  Leu Gly Leu Leu Ala  Gly Pro 
    1145                 1150         


<210>  11
<211>  226
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide having GDIH at N-terminal of PPTase peptide 
       from Nocardia NRRL5646.

<400>  11

Gly Asp Ile His Met Ile Glu Thr Ile Leu Pro Ala Gly Val Glu Ser 
1               5                   10                  15      


Ala Glu Leu Leu Glu Tyr Pro Glu Asp Leu Lys Ala His Pro Ala Glu 
            20                  25                  30          


Glu His Leu Ile Ala Lys Ser Val Glu Lys Arg Arg Arg Asp Phe Ile 
        35                  40                  45              


Gly Ala Arg His Cys Ala Arg Leu Ala Leu Ala Glu Leu Gly Glu Pro 
    50                  55                  60                  


Pro Val Ala Ile Gly Lys Gly Glu Arg Gly Ala Pro Ile Trp Pro Arg 
65                  70                  75                  80  


Gly Val Val Gly Ser Leu Thr His Cys Asp Gly Tyr Arg Ala Ala Ala 
                85                  90                  95      


Val Ala His Lys Met Arg Phe Arg Ser Ile Gly Ile Asp Ala Glu Pro 
            100                 105                 110         


His Ala Thr Leu Pro Glu Gly Val Leu Asp Ser Val Ser Leu Pro Pro 
        115                 120                 125             


Glu Arg Glu Trp Leu Lys Thr Thr Asp Ser Ala Leu His Leu Asp Arg 
    130                 135                 140                 


Leu Leu Phe Cys Ala Lys Glu Ala Thr Tyr Lys Ala Trp Trp Pro Leu 
145                 150                 155                 160 


Thr Ala Arg Trp Leu Gly Phe Glu Glu Ala His Ile Thr Phe Glu Ile 
                165                 170                 175     


Glu Asp Gly Ser Ala Asp Ser Gly Asn Gly Thr Phe His Ser Glu Leu 
            180                 185                 190         


Leu Val Pro Gly Gln Thr Asn Asp Gly Gly Thr Pro Leu Leu Ser Phe 
        195                 200                 205             


Asp Gly Arg Trp Leu Ile Ala Asp Gly Phe Ile Leu Thr Ala Ile Ala 
    210                 215                 220                 


Tyr Ala 
225     


