                               SEQUENCE LISTING	

<110> UNIVERSITY OF MARYLAND, BALTIMORE
 
<120> ENGINEERED TYPE IV PILIN OF CLOSTRIDIUM DIFFICILE

<130> UMB-008PCT1

<140>
<141>

<150> 61/179,747
<151> 2009-05-20

<160> 44    

<170> PatentIn version 3.5

<210> 1
<211> 522
<212> DNA
<213> Clostridium difficile

<400> 1
atgaagttaa aaaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca       60

attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaacaaggct      120

aaggtagcaa gtgttgagtc tgattatagt tcaattaaga gtgcagcatt atcttattat      180

tcagatacta ataaaatacc agttacacca gatggtcaaa ctggtttaaa tgttttagag      240

acttatatgg aatctcttcc tgataaagct gatataggtg gagaatataa attgattaaa      300

gttggtaata aattagtatt acagataggt aaagatggtg aaggagttac cttaacagaa      360

gcgcaatcag caaaattatt gagtgatata ggtaaagata aaatatatac aggtgttaca      420

ggagataatt ttggagagca attaaaagat actacaaaaa tagataataa agctctatat      480

atagtactta tagataatac tgtgatggat tcaacaaaat ag                         522


<210> 2
<211> 173
<212> PRT
<213> Clostridium difficile

<400> 2
Met Lys Leu Lys Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu 
1               5                   10                  15      


Val Val Ile Ala Ile Ile Gly Ile Leu Ala Val Val Ala Val Pro Ala 
            20                  25                  30          


Leu Phe Ser Asn Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp 
        35                  40                  45              


Tyr Ser Ser Ile Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn 
    50                  55                  60                  


Lys Ile Pro Val Thr Pro Asp Gly Gln Thr Gly Leu Asn Val Leu Glu 
65                  70                  75                  80  


Thr Tyr Met Glu Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr 
                85                  90                  95      


Lys Leu Ile Lys Val Gly Asn Lys Leu Val Leu Gln Ile Gly Lys Asp 
            100                 105                 110         


Gly Glu Gly Val Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser 
        115                 120                 125             


Asp Ile Gly Lys Asp Lys Ile Tyr Thr Gly Val Thr Gly Asp Asn Phe 
    130                 135                 140                 


Gly Glu Gln Leu Lys Asp Thr Thr Lys Ile Asp Asn Lys Ala Leu Tyr 
145                 150                 155                 160 


Ile Val Leu Ile Asp Asn Thr Val Met Asp Ser Thr Lys 
                165                 170             


<210> 3
<211> 558
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggttc taacatcaac aaagctaaag ttgcttctgt tgaatctgac      180

tactcttcta tcaaatctgc tgctctgtct tactactctg acaccaacaa aatcccggtt      240

accccggacg gtcagaccgg tctgaacgtt ctggaaacct acatggaatc tctgccggac      300

aaagctgaca tcggtggtga atacaaactg atcaaagttg gtaacaaact ggttctgcag      360

atcggtaaag acggtgaagg tgttaccctg accgaagctc agtctgctaa actgctgtct      420

gacatcggta aagacaaaat ctacaccggt gttaccggtg acaacttcgg tgaacagctg      480

aaagacacca ccaaaatcga caacaaagct ctgtacatcg ttctgatcga caacaccgtt      540

atggactcta ccaaatag                                                    558


<210> 4
<211> 185
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Ser Asn 
        35                  40                  45              


Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp Tyr Ser Ser Ile 
    50                  55                  60                  


Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn Lys Ile Pro Val 
65                  70                  75                  80  


Thr Pro Asp Gly Gln Thr Gly Leu Asn Val Leu Glu Thr Tyr Met Glu 
                85                  90                  95      


Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr Lys Leu Ile Lys 
            100                 105                 110         


Val Gly Asn Lys Leu Val Leu Gln Ile Gly Lys Asp Gly Glu Gly Val 
        115                 120                 125             


Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser Asp Ile Gly Lys 
    130                 135                 140                 


Asp Lys Ile Tyr Thr Gly Val Thr Gly Asp Asn Phe Gly Glu Gln Leu 
145                 150                 155                 160 


Lys Asp Thr Thr Lys Ile Asp Asn Lys Ala Leu Tyr Ile Val Leu Ile 
                165                 170                 175     


Asp Asn Thr Val Met Asp Ser Thr Lys 
            180                 185 


<210> 5
<211> 522
<212> DNA
<213> Clostridium difficile

<400> 5
atgaagttaa aaaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca       60

attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaacaaggct      120

aaggtagcaa gtgttgagtc tgattatagt tcagttaaga gtgctgcatt atcttattat      180

tcagatacta ataagatacc agttacacca gatggtcaaa ctggtttaag tgttttagaa      240

acttatatgg agtctcttcc tgataaagct gatataggtg gagaatataa attgattaaa      300

gttggtagta aattggtatt acagataggt acaaatactg agggagttac cttaacagaa      360

gcacaatcag caaaattatt gagtgatata ggtgaaaaaa aaatatatac aagcgctaca      420

acaaatagtt tgggagatcc attaacaagt aatacaaaaa tagataataa agttctatat      480

atagtactta tagataatac tgtgatggac acaacaaaat ag                         522


<210> 6
<211> 173
<212> PRT
<213> Clostridium difficile

<400> 6
Met Lys Leu Lys Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu 
1               5                   10                  15      


Val Val Ile Ala Ile Ile Gly Ile Leu Ala Val Val Ala Val Pro Ala 
            20                  25                  30          


Leu Phe Ser Asn Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp 
        35                  40                  45              


Tyr Ser Ser Val Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn 
    50                  55                  60                  


Lys Ile Pro Val Thr Pro Asp Gly Gln Thr Gly Leu Ser Val Leu Glu 
65                  70                  75                  80  


Thr Tyr Met Glu Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr 
                85                  90                  95      


Lys Leu Ile Lys Val Gly Ser Lys Leu Val Leu Gln Ile Gly Thr Asn 
            100                 105                 110         


Thr Glu Gly Val Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser 
        115                 120                 125             


Asp Ile Gly Glu Lys Lys Ile Tyr Thr Ser Ala Thr Thr Asn Ser Leu 
    130                 135                 140                 


Gly Asp Pro Leu Thr Ser Asn Thr Lys Ile Asp Asn Lys Val Leu Tyr 
145                 150                 155                 160 


Ile Val Leu Ile Asp Asn Thr Val Met Asp Thr Thr Lys 
                165                 170             


<210> 7
<211> 558
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 7
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggttc taacatcaac aaagctaaag ttgcttctgt tgaatctgac      180

tactcttctg ttaaatctgc tgctctgtct tactactctg acaccaacaa aatcccggtt      240

accccggacg gtcagaccgg tctgtctgtt ctggaaacct acatggaatc tctgccggac      300

aaagctgaca tcggtggtga atacaaactg atcaaagttg gttctaaact ggttctgcag      360

atcggtacca acaccgaagg tgttaccctg accgaagctc agtctgctaa actgctgtct      420

gacatcggtg aaaaaaaaat ctacacctct gctaccacca actctctggg tgacccgctg      480

acctctaaca ccaaaatcga caacaaagtt ctgtacatcg ttctgatcga caacaccgtt      540

atggacacca ccaaatag                                                    558


<210> 8
<211> 185
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Ser Asn 
        35                  40                  45              


Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp Tyr Ser Ser Val 
    50                  55                  60                  


Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn Lys Ile Pro Val 
65                  70                  75                  80  


Thr Pro Asp Gly Gln Thr Gly Leu Ser Val Leu Glu Thr Tyr Met Glu 
                85                  90                  95      


Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr Lys Leu Ile Lys 
            100                 105                 110         


Val Gly Ser Lys Leu Val Leu Gln Ile Gly Thr Asn Thr Glu Gly Val 
        115                 120                 125             


Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser Asp Ile Gly Glu 
    130                 135                 140                 


Lys Lys Ile Tyr Thr Ser Ala Thr Thr Asn Ser Leu Gly Asp Pro Leu 
145                 150                 155                 160 


Thr Ser Asn Thr Lys Ile Asp Asn Lys Val Leu Tyr Ile Val Leu Ile 
                165                 170                 175     


Asp Asn Thr Val Met Asp Thr Thr Lys 
            180                 185 


<210> 9
<211> 522
<212> DNA
<213> Clostridium difficile

<400> 9
atgaagttaa aaaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca       60

attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaacaaggct      120

aaggtagcaa gtgttgagtc tgattatagt tcaattaaga gtgcagcatt atcttattat      180

tcagatacta ataaaatacc agttacacca gatggtcaaa ctggtttaaa tgttttagag      240

acttatatgg aatctcttcc tgataaagct gatataggtg gagaatataa attgattaaa      300

gttggtaata aattagtatt acagataggt aaagatggtg aaggagttac cttaacagaa      360

gcgcaatcag caaaattatt gagtgatata ggtaaagata aaatatatac aggtgttaca      420

ggagataatt ttggagagca attaaaagat actacaaaaa tagataataa agctctatat      480

atagtactta tagataatac tgtgatggat tcaacaaaat ag                         522


<210> 10
<211> 173
<212> PRT
<213> Clostridium difficile

<400> 10
Met Lys Leu Lys Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu 
1               5                   10                  15      


Val Val Ile Ala Ile Ile Gly Ile Leu Ala Val Val Ala Val Pro Ala 
            20                  25                  30          


Leu Phe Ser Asn Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp 
        35                  40                  45              


Tyr Ser Ser Ile Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn 
    50                  55                  60                  


Lys Ile Pro Val Thr Pro Asp Gly Gln Thr Gly Leu Asn Val Leu Glu 
65                  70                  75                  80  


Thr Tyr Met Glu Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr 
                85                  90                  95      


Lys Leu Ile Lys Val Gly Asn Lys Leu Val Leu Gln Ile Gly Lys Asp 
            100                 105                 110         


Gly Glu Gly Val Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser 
        115                 120                 125             


Asp Ile Gly Lys Asp Lys Ile Tyr Thr Gly Val Thr Gly Asp Asn Phe 
    130                 135                 140                 


Gly Glu Gln Leu Lys Asp Thr Thr Lys Ile Asp Asn Lys Ala Leu Tyr 
145                 150                 155                 160 


Ile Val Leu Ile Asp Asn Thr Val Met Asp Ser Thr Lys 
                165                 170             


<210> 11
<211> 558
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 11
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggttc taacatcaac aaagctaaag ttgcttctgt tgaatctgac      180

tactcttcta tcaaatctgc tgctctgtct tactactctg acaccaacaa aatcccggtt      240

accccggacg gtcagaccgg tctgaacgtt ctggaaacct acatggaatc tctgccggac      300

aaagctgaca tcggtggtga atacaaactg atcaaagttg gtaacaaact ggttctgcag      360

atcggtaaag acggtgaagg tgttaccctg accgaagctc agtctgctaa actgctgtct      420

gacatcggta aagacaaaat ctacaccggt gttaccggtg acaacttcgg tgaacagctg      480

aaagacacca ccaaaatcga caacaaagct ctgtacatcg ttctgatcga caacaccgtt      540

atggactcta ccaaatag                                                    558


<210> 12
<211> 185
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Ser Asn 
        35                  40                  45              


Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp Tyr Ser Ser Ile 
    50                  55                  60                  


Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn Lys Ile Pro Val 
65                  70                  75                  80  


Thr Pro Asp Gly Gln Thr Gly Leu Asn Val Leu Glu Thr Tyr Met Glu 
                85                  90                  95      


Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr Lys Leu Ile Lys 
            100                 105                 110         


Val Gly Asn Lys Leu Val Leu Gln Ile Gly Lys Asp Gly Glu Gly Val 
        115                 120                 125             


Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser Asp Ile Gly Lys 
    130                 135                 140                 


Asp Lys Ile Tyr Thr Gly Val Thr Gly Asp Asn Phe Gly Glu Gln Leu 
145                 150                 155                 160 


Lys Asp Thr Thr Lys Ile Asp Asn Lys Ala Leu Tyr Ile Val Leu Ile 
                165                 170                 175     


Asp Asn Thr Val Met Asp Ser Thr Lys 
            180                 185 


<210> 13
<211> 516
<212> DNA
<213> Clostridium difficile

<400> 13
atgaagttaa aaaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca       60

attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaacaaggct      120

aaggtagcaa gtgttgagtc tgattatagt tcagttaaga gtgcagcatt atcttattat      180

tcagatacta ataagatacc agttacacca gatggtcaaa ctggtttaag tgttttagag      240

acttatatgg agtctctgcc tgataaagct gatataggtg gaaaatataa attgattaaa      300

gttggtaata aattggtatt acagataggt acaaatactg aaggagttac cttaacagaa      360

gcacaatcag caaaattatt gagtgatata ggtgaaaata aaatatatac aaatgcagct      420

cttagtgcta aattaacatc tactacaaag gtaaataatg aagctttata tatagttctt      480

atagataata ttgtaatgga tcaacaagga gcttaa                                516


<210> 14
<211> 171
<212> PRT
<213> Clostridium difficile

<400> 14
Met Lys Leu Lys Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu 
1               5                   10                  15      


Val Val Ile Ala Ile Ile Gly Ile Leu Ala Val Val Ala Val Pro Ala 
            20                  25                  30          


Leu Phe Ser Asn Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp 
        35                  40                  45              


Tyr Ser Ser Val Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn 
    50                  55                  60                  


Lys Ile Pro Val Thr Pro Asp Gly Gln Thr Gly Leu Ser Val Leu Glu 
65                  70                  75                  80  


Thr Tyr Met Glu Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Lys Tyr 
                85                  90                  95      


Lys Leu Ile Lys Val Gly Asn Lys Leu Val Leu Gln Ile Gly Thr Asn 
            100                 105                 110         


Thr Glu Gly Val Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser 
        115                 120                 125             


Asp Ile Gly Glu Asn Lys Ile Tyr Thr Asn Ala Ala Leu Ser Ala Lys 
    130                 135                 140                 


Leu Thr Ser Thr Thr Lys Val Asn Asn Glu Ala Leu Tyr Ile Val Leu 
145                 150                 155                 160 


Ile Asp Asn Ile Val Met Asp Gln Gln Gly Ala 
                165                 170     


<210> 15
<211> 552
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 15
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggttc taacatcaac aaagctaaag ttgcttctgt tgaatctgac      180

tactcttctg ttaaatctgc tgctctgtct tactactctg acaccaacaa aatcccggtt      240

accccggacg gtcagaccgg tctgtctgtt ctggaaacct acatggaatc tctgccggac      300

aaagctgaca tcggtggtaa atacaaactg atcaaagttg gtaacaaact ggttctgcag      360

atcggtacca acaccgaagg tgttaccctg accgaagctc agtctgctaa actgctgtct      420

gacatcggtg aaaacaaaat ctacaccaac gctgctctgt ctgctaaact gacctctacc      480

accaaagtta acaacgaagc tctgtacatc gttctgatcg acaacatcgt tatggaccag      540

cagggtgctt aa                                                          552


<210> 16
<211> 183
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Ser Asn 
        35                  40                  45              


Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp Tyr Ser Ser Val 
    50                  55                  60                  


Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn Lys Ile Pro Val 
65                  70                  75                  80  


Thr Pro Asp Gly Gln Thr Gly Leu Ser Val Leu Glu Thr Tyr Met Glu 
                85                  90                  95      


Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Lys Tyr Lys Leu Ile Lys 
            100                 105                 110         


Val Gly Asn Lys Leu Val Leu Gln Ile Gly Thr Asn Thr Glu Gly Val 
        115                 120                 125             


Thr Leu Thr Glu Ala Gln Ser Ala Lys Leu Leu Ser Asp Ile Gly Glu 
    130                 135                 140                 


Asn Lys Ile Tyr Thr Asn Ala Ala Leu Ser Ala Lys Leu Thr Ser Thr 
145                 150                 155                 160 


Thr Lys Val Asn Asn Glu Ala Leu Tyr Ile Val Leu Ile Asp Asn Ile 
                165                 170                 175     


Val Met Asp Gln Gln Gly Ala 
            180             


<210> 17
<211> 513
<212> DNA
<213> Clostridium difficile

<400> 17
atgaagttaa agaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca       60

attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaataaggct      120

aaggtagcaa gtgttgagtc tgattatagt tcaattaaga gtgcagcatt atcttattat      180

tcagatacta ataagatgcc agctacaaca tcaaatcctg tagatttaga aaatttaaaa      240

acttatatgg aaagtcttcc tgataaagca gatataggtg gagagtatca attacttttg      300

gttgggaata agttagtttt acaaataaat gatgctacat taacaggagc gcaatcaacg      360

aagttattga gtgatttagg taatgataag atatacaaaa ctataggtag cgatgataag      420

cttacagatt tattaactac caatgaaaaa ttagataata aggttctata tttagttctt      480

atagataatg ctgagatgga ttcaacaaaa taa                                   513


<210> 18
<211> 170
<212> PRT
<213> Clostridium difficile

<400> 18
Met Lys Leu Lys Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu 
1               5                   10                  15      


Val Val Ile Ala Ile Ile Gly Ile Leu Ala Val Val Ala Val Pro Ala 
            20                  25                  30          


Leu Phe Ser Asn Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp 
        35                  40                  45              


Tyr Ser Ser Ile Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn 
    50                  55                  60                  


Lys Met Pro Ala Thr Thr Ser Asn Pro Val Asp Leu Glu Asn Leu Lys 
65                  70                  75                  80  


Thr Tyr Met Glu Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr 
                85                  90                  95      


Gln Leu Leu Leu Val Gly Asn Lys Leu Val Leu Gln Ile Asn Asp Ala 
            100                 105                 110         


Thr Leu Thr Gly Ala Gln Ser Thr Lys Leu Leu Ser Asp Leu Gly Asn 
        115                 120                 125             


Asp Lys Ile Tyr Lys Thr Ile Gly Ser Asp Asp Lys Leu Thr Asp Leu 
    130                 135                 140                 


Leu Thr Thr Asn Glu Lys Leu Asp Asn Lys Val Leu Tyr Leu Val Leu 
145                 150                 155                 160 


Ile Asp Asn Ala Glu Met Asp Ser Thr Lys 
                165                 170 


<210> 19
<211> 549
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 19
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggttc taacatcaac aaagctaaag ttgcttctgt tgaatctgac      180

tactcttcta tcaaatctgc tgctctgtct tactactctg acaccaacaa aatgccggct      240

accacctcta acccggttga cctggaaaac ctgaaaacct acatggaatc tctgccggac      300

aaagctgaca tcggtggtga ataccagctg ctgctggttg gtaacaaact ggttctgcag      360

atcaacgacg ctaccctgac cggtgctcag tctaccaaac tgctgtctga cctgggtaac      420

gacaaaatct acaaaaccat cggttctgac gacaaactga ccgacctgct gaccaccaac      480

gaaaaactgg acaacaaagt tctgtacctg gttctgatcg acaacgctga aatggactct      540

accaaataa                                                              549


<210> 20
<211> 182
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 20
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Ser Asn 
        35                  40                  45              


Ile Asn Lys Ala Lys Val Ala Ser Val Glu Ser Asp Tyr Ser Ser Ile 
    50                  55                  60                  


Lys Ser Ala Ala Leu Ser Tyr Tyr Ser Asp Thr Asn Lys Met Pro Ala 
65                  70                  75                  80  


Thr Thr Ser Asn Pro Val Asp Leu Glu Asn Leu Lys Thr Tyr Met Glu 
                85                  90                  95      


Ser Leu Pro Asp Lys Ala Asp Ile Gly Gly Glu Tyr Gln Leu Leu Leu 
            100                 105                 110         


Val Gly Asn Lys Leu Val Leu Gln Ile Asn Asp Ala Thr Leu Thr Gly 
        115                 120                 125             


Ala Gln Ser Thr Lys Leu Leu Ser Asp Leu Gly Asn Asp Lys Ile Tyr 
    130                 135                 140                 


Lys Thr Ile Gly Ser Asp Asp Lys Leu Thr Asp Leu Leu Thr Thr Asn 
145                 150                 155                 160 


Glu Lys Leu Asp Asn Lys Val Leu Tyr Leu Val Leu Ile Asp Asn Ala 
                165                 170                 175     


Glu Met Asp Ser Thr Lys 
            180         


<210> 21
<211> 360
<212> DNA
<213> Clostridium difficile

<400> 21
ttgataaatt tgataaataa aaaacgaaaa ggatttacac ttgttgaaat gattgtagta       60

gtaactattt taggcgttat atctagtata gcattagtta agtatagtaa ggttcaagaa      120

agtgccaaat taaatgcaga ctatacgaat gctgctaata tagtaactgc agctagcatg      180

gcaattaatg atgatgaaaa gacaatagac tctctaagtg tagaaacatt gaaggaaaag      240

ggatacctaa atactgttcc agttcctcag agtacatcag gtaaattcga acttgtcata      300

aatgatagcg gaacagatat aagcgtaaat ataaattcta aacaatttta tccaaaataa      360


<210> 22
<211> 119
<212> PRT
<213> Clostridium difficile

<400> 22
Met Ile Asn Leu Ile Asn Lys Lys Arg Lys Gly Phe Thr Leu Val Glu 
1               5                   10                  15      


Met Ile Val Val Val Thr Ile Leu Gly Val Ile Ser Ser Ile Ala Leu 
            20                  25                  30          


Val Lys Tyr Ser Lys Val Gln Glu Ser Ala Lys Leu Asn Ala Asp Tyr 
        35                  40                  45              


Thr Asn Ala Ala Asn Ile Val Thr Ala Ala Ser Met Ala Ile Asn Asp 
    50                  55                  60                  


Asp Glu Lys Thr Ile Asp Ser Leu Ser Val Glu Thr Leu Lys Glu Lys 
65                  70                  75                  80  


Gly Tyr Leu Asn Thr Val Pro Val Pro Gln Ser Thr Ser Gly Lys Phe 
                85                  90                  95      


Glu Leu Val Ile Asn Asp Ser Gly Thr Asp Ile Ser Val Asn Ile Asn 
            100                 105                 110         


Ser Lys Gln Phe Tyr Pro Lys 
        115                 


<210> 23
<211> 399
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 23
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtaa atactctaaa gttcaggaat ctgctaaact gaacgctgac      180

tacaccaacg ctgctaacat cgttaccgct gcttctatgg ctatcaacga cgacgaaaaa      240

accatcgact ctctgtctgt tgaaaccctg aaagaaaaag gttacctgaa caccgttccg      300

gttccgcagt ctacctctgg taaattcgaa ctggttatca acgactctgg taccgacatc      360

tctgttaaca tcaactctaa acagttctac ccgaaataa                             399


<210> 24
<211> 132
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 24
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Lys Tyr 
        35                  40                  45              


Ser Lys Val Gln Glu Ser Ala Lys Leu Asn Ala Asp Tyr Thr Asn Ala 
    50                  55                  60                  


Ala Asn Ile Val Thr Ala Ala Ser Met Ala Ile Asn Asp Asp Glu Lys 
65                  70                  75                  80  


Thr Ile Asp Ser Leu Ser Val Glu Thr Leu Lys Glu Lys Gly Tyr Leu 
                85                  90                  95      


Asn Thr Val Pro Val Pro Gln Ser Thr Ser Gly Lys Phe Glu Leu Val 
            100                 105                 110         


Ile Asn Asp Ser Gly Thr Asp Ile Ser Val Asn Ile Asn Ser Lys Gln 
        115                 120                 125             


Phe Tyr Pro Lys 
    130         


<210> 25
<211> 351
<212> DNA
<213> Clostridium difficile

<400> 25
ttgataaata aaaaacgaaa aggatttaca cttgttgaaa tgattgtagt agtaactatt       60

ttaggagtta tatctagtat agcattagtt aagtatagta aggttcaaga aagtgctaaa      120

ttaaatgcag actatacgaa tgctgctaat atagtaacag cagctagtat ggcaattaat      180

gatgatgaaa atataataga ctctctaagt gtagaagcat tgaaggaaaa gggataccta      240

aatactgttc cagttcctca gagtacatca ggtaaattcg aacttgttat aaatgataac      300

ggaacagata taagcgtgaa tataaattct aagcaatttt atccaaaata a               351


<210> 26
<211> 116
<212> PRT
<213> Clostridium difficile

<400> 26
Met Ile Asn Lys Lys Arg Lys Gly Phe Thr Leu Val Glu Met Ile Val 
1               5                   10                  15      


Val Val Thr Ile Leu Gly Val Ile Ser Ser Ile Ala Leu Val Lys Tyr 
            20                  25                  30          


Ser Lys Val Gln Glu Ser Ala Lys Leu Asn Ala Asp Tyr Thr Asn Ala 
        35                  40                  45              


Ala Asn Ile Val Thr Ala Ala Ser Met Ala Ile Asn Asp Asp Glu Asn 
    50                  55                  60                  


Ile Ile Asp Ser Leu Ser Val Glu Ala Leu Lys Glu Lys Gly Tyr Leu 
65                  70                  75                  80  


Asn Thr Val Pro Val Pro Gln Ser Thr Ser Gly Lys Phe Glu Leu Val 
                85                  90                  95      


Ile Asn Asp Asn Gly Thr Asp Ile Ser Val Asn Ile Asn Ser Lys Gln 
            100                 105                 110         


Phe Tyr Pro Lys 
        115     


<210> 27
<211> 399
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 27
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtaa atactctaaa gttcaggaat ctgctaaact gaacgctgac      180

tacaccaacg ctgctaacat cgttaccgct gcttctatgg ctatcaacga cgacgaaaac      240

atcatcgact ctctgtctgt tgaagctctg aaagaaaaag gttacctgaa caccgttccg      300

gttccgcagt ctacctctgg taaattcgaa ctggttatca acgacaacgg taccgacatc      360

tctgttaaca tcaactctaa acagttctac ccgaaataa                             399


<210> 28
<211> 132
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 28
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Lys Tyr 
        35                  40                  45              


Ser Lys Val Gln Glu Ser Ala Lys Leu Asn Ala Asp Tyr Thr Asn Ala 
    50                  55                  60                  


Ala Asn Ile Val Thr Ala Ala Ser Met Ala Ile Asn Asp Asp Glu Asn 
65                  70                  75                  80  


Ile Ile Asp Ser Leu Ser Val Glu Ala Leu Lys Glu Lys Gly Tyr Leu 
                85                  90                  95      


Asn Thr Val Pro Val Pro Gln Ser Thr Ser Gly Lys Phe Glu Leu Val 
            100                 105                 110         


Ile Asn Asp Asn Gly Thr Asp Ile Ser Val Asn Ile Asn Ser Lys Gln 
        115                 120                 125             


Phe Tyr Pro Lys 
    130         


<210> 29
<211> 495
<212> DNA
<213> Clostridium difficile

<400> 29
atgaaaaata aaaaaggatt tactctagtg gaattattag tagtaattgc tataatagga       60

atattggcaa taatagcact tccagcatta tttaaaaata tagaaaaagc aaagatagct      120

aaacttgaag ctgatataag tgcaataaaa agtgcatctc ttagttacta tgctgatgaa      180

tccaagtata ctgatggagg aatgatatca tgggtaaaga aagatggaaa aataataata      240

aatgggggtt ttaaagatga cccattagca gataaaatag aaaatttagg gatgccttat      300

aatggttcat atctgttaat gtcatctcct ggtcatgaaa aatatctaga attaagcata      360

cttccagaag gagaaataag caaaagtggt ctagataaat taaaaaatga ttatggaaat      420

ttaatagaca taacgaacga tcaaaataaa ataaatattg taataaaact tttaaataat      480

aaatcgaata cttaa                                                       495


<210> 30
<211> 164
<212> PRT
<213> Clostridium difficile

<400> 30
Met Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu Val Val Ile 
1               5                   10                  15      


Ala Ile Ile Gly Ile Leu Ala Ile Ile Ala Leu Pro Ala Leu Phe Lys 
            20                  25                  30          


Asn Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala 
        35                  40                  45              


Ile Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr 
    50                  55                  60                  


Asp Gly Gly Met Ile Ser Trp Val Lys Lys Asp Gly Lys Ile Ile Ile 
65                  70                  75                  80  


Asn Gly Gly Phe Lys Asp Asp Pro Leu Ala Asp Lys Ile Glu Asn Leu 
                85                  90                  95      


Gly Met Pro Tyr Asn Gly Ser Tyr Leu Leu Met Ser Ser Pro Gly His 
            100                 105                 110         


Glu Lys Tyr Leu Glu Leu Ser Ile Leu Pro Glu Gly Glu Ile Ser Lys 
        115                 120                 125             


Ser Gly Leu Asp Lys Leu Lys Asn Asp Tyr Gly Asn Leu Ile Asp Ile 
    130                 135                 140                 


Thr Asn Asp Gln Asn Lys Ile Asn Ile Val Ile Lys Leu Leu Asn Asn 
145                 150                 155                 160 


Lys Ser Asn Thr 
                


<210> 31
<211> 540
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 31
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtaa aaacatcgaa aaagctaaaa tcgctaaact ggaagctgac      180

atctctgcta tcaaatctgc ttctctgtct tactacgctg acgaatctaa atacaccgac      240

ggtggtatga tctcttgggt taaaaaagac ggtaaaatca tcatcaacgg tggtttcaaa      300

gacgacccgc tggctgacaa aatcgaaaac ctgggtatgc cgtacaacgg ttcttacctg      360

ctgatgtctt ctccgggtca cgaaaaatac ctggaactgt ctatcctgcc ggaaggtgaa      420

atctctaaat ctggtctgga caaactgaaa aacgactacg gtaacctgat cgacatcacc      480

aacgaccaga acaaaatcaa catcgttatc aaactgctga acaacaaatc taacacctaa      540


<210> 32
<211> 179
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 32
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Lys Asn 
        35                  40                  45              


Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala Ile 
    50                  55                  60                  


Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr Asp 
65                  70                  75                  80  


Gly Gly Met Ile Ser Trp Val Lys Lys Asp Gly Lys Ile Ile Ile Asn 
                85                  90                  95      


Gly Gly Phe Lys Asp Asp Pro Leu Ala Asp Lys Ile Glu Asn Leu Gly 
            100                 105                 110         


Met Pro Tyr Asn Gly Ser Tyr Leu Leu Met Ser Ser Pro Gly His Glu 
        115                 120                 125             


Lys Tyr Leu Glu Leu Ser Ile Leu Pro Glu Gly Glu Ile Ser Lys Ser 
    130                 135                 140                 


Gly Leu Asp Lys Leu Lys Asn Asp Tyr Gly Asn Leu Ile Asp Ile Thr 
145                 150                 155                 160 


Asn Asp Gln Asn Lys Ile Asn Ile Val Ile Lys Leu Leu Asn Asn Lys 
                165                 170                 175     


Ser Asn Thr 
            


<210> 33
<211> 495
<212> DNA
<213> Clostridium difficile

<400> 33
atgaaaaata aaaaaggatt tactctagtg gaattattag tagtaattgc tataatagga       60

atattggcaa tagtagcact tccagcatta tttaaaaata tagaaaaagc aaagatagct      120

aaacttgaag ctgatataag tgcaataaaa agtgcgtctc ttagctacta tgcagatgaa      180

tcaaaatata ctgatggagg aatgatatca tgggtaaaga aagatggaaa aataataata      240

aatggtggct ttaaagatga cccattagca gataaaatag aaaatttagg tatgccttat      300

aatggttcat atctattaat gtcatctcct ggtcatgaaa aatatctaga attaagtata      360

cttccagaag gagaaataag caaaagtggt ctagataaat taaaaagtga ttatggaagt      420

tcaatagaca taaagaacga tcaaaacaaa atagatattg taataaaact tttaaatgat      480

aaatcgaata cttaa                                                       495


<210> 34
<211> 164
<212> PRT
<213> Clostridium difficile

<400> 34
Met Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu Val Val Ile 
1               5                   10                  15      


Ala Ile Ile Gly Ile Leu Ala Ile Val Ala Leu Pro Ala Leu Phe Lys 
            20                  25                  30          


Asn Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala 
        35                  40                  45              


Ile Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr 
    50                  55                  60                  


Asp Gly Gly Met Ile Ser Trp Val Lys Lys Asp Gly Lys Ile Ile Ile 
65                  70                  75                  80  


Asn Gly Gly Phe Lys Asp Asp Pro Leu Ala Asp Lys Ile Glu Asn Leu 
                85                  90                  95      


Gly Met Pro Tyr Asn Gly Ser Tyr Leu Leu Met Ser Ser Pro Gly His 
            100                 105                 110         


Glu Lys Tyr Leu Glu Leu Ser Ile Leu Pro Glu Gly Glu Ile Ser Lys 
        115                 120                 125             


Ser Gly Leu Asp Lys Leu Lys Ser Asp Tyr Gly Ser Ser Ile Asp Ile 
    130                 135                 140                 


Lys Asn Asp Gln Asn Lys Ile Asp Ile Val Ile Lys Leu Leu Asn Asp 
145                 150                 155                 160 


Lys Ser Asn Thr 
                


<210> 35
<211> 540
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 35
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtaa aaacatcgaa aaagctaaaa tcgctaaact ggaagctgac      180

atctctgcta tcaaatctgc ttctctgtct tactacgctg acgaatctaa atacaccgac      240

ggtggtatga tctcttgggt taaaaaagac ggtaaaatca tcatcaacgg tggtttcaaa      300

gacgacccgc tggctgacaa aatcgaaaac ctgggtatgc cgtacaacgg ttcttacctg      360

ctgatgtctt ctccgggtca cgaaaaatac ctggaactgt ctatcctgcc ggaaggtgaa      420

atctctaaat ctggtctgga caaactgaaa tctgactacg gttcttctat cgacatcaaa      480

aacgaccaga acaaaatcga catcgttatc aaactgctga acgacaaatc taacacctaa      540


<210> 36
<211> 179
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 36
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Lys Asn 
        35                  40                  45              


Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala Ile 
    50                  55                  60                  


Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr Asp 
65                  70                  75                  80  


Gly Gly Met Ile Ser Trp Val Lys Lys Asp Gly Lys Ile Ile Ile Asn 
                85                  90                  95      


Gly Gly Phe Lys Asp Asp Pro Leu Ala Asp Lys Ile Glu Asn Leu Gly 
            100                 105                 110         


Met Pro Tyr Asn Gly Ser Tyr Leu Leu Met Ser Ser Pro Gly His Glu 
        115                 120                 125             


Lys Tyr Leu Glu Leu Ser Ile Leu Pro Glu Gly Glu Ile Ser Lys Ser 
    130                 135                 140                 


Gly Leu Asp Lys Leu Lys Ser Asp Tyr Gly Ser Ser Ile Asp Ile Lys 
145                 150                 155                 160 


Asn Asp Gln Asn Lys Ile Asp Ile Val Ile Lys Leu Leu Asn Asp Lys 
                165                 170                 175     


Ser Asn Thr 
            


<210> 37
<211> 489
<212> DNA
<213> Clostridium difficile

<400> 37
atgaaaaata aaaaaggatt tactctagtg gaattattag tagtaattgc tataatagga       60

atattggcaa tagtagcact tccagcatta tttaaaaata tagaaaaagc aaagatagct      120

aaacttgaag ctgatataag tgcaataaaa agtgcgtctc ttagctacta tgcagatgaa      180

tcaaaatata ctgaaggaaa cataatatgg tggactaaaa aagatggaaa aataacagta      240

aactctggta ttggtgatga agaccctttg gcacataaaa tagaaaattt aggcatgcct      300

tataatggtt cgtacacttt agtgtcatct aatggtagtg aagaatactt agaattaaac      360

ataattatag atggagaaat aagtaaaagt ggtctagata aattagaaga agattatggt      420

agttcaataa caataccaaa tgataaaaat atgataataa cttttttatc taataaatca      480

gacaattaa                                                              489


<210> 38
<211> 162
<212> PRT
<213> Clostridium difficile

<400> 38
Met Lys Asn Lys Lys Gly Phe Thr Leu Val Glu Leu Leu Val Val Ile 
1               5                   10                  15      


Ala Ile Ile Gly Ile Leu Ala Ile Val Ala Leu Pro Ala Leu Phe Lys 
            20                  25                  30          


Asn Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala 
        35                  40                  45              


Ile Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr 
    50                  55                  60                  


Glu Gly Asn Ile Ile Trp Trp Thr Lys Lys Asp Gly Lys Ile Thr Val 
65                  70                  75                  80  


Asn Ser Gly Ile Gly Asp Glu Asp Pro Leu Ala His Lys Ile Glu Asn 
                85                  90                  95      


Leu Gly Met Pro Tyr Asn Gly Ser Tyr Thr Leu Val Ser Ser Asn Gly 
            100                 105                 110         


Ser Glu Glu Tyr Leu Glu Leu Asn Ile Ile Ile Asp Gly Glu Ile Ser 
        115                 120                 125             


Lys Ser Gly Leu Asp Lys Leu Glu Glu Asp Tyr Gly Ser Ser Ile Thr 
    130                 135                 140                 


Ile Pro Asn Asp Lys Asn Met Ile Ile Thr Phe Leu Ser Asn Lys Ser 
145                 150                 155                 160 


Asp Asn 
        


<210> 39
<211> 534
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 39
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtaa aaacatcgaa aaagctaaaa tcgctaaact ggaagctgac      180

atctctgcta tcaaatctgc ttctctgtct tactacgctg acgaatctaa atacaccgaa      240

ggtaacatca tctggtggac caaaaaagac ggtaaaatca ccgttaactc tggtatcggt      300

gacgaagacc cgctggctca caaaatcgaa aacctgggta tgccgtacaa cggttcttac      360

accctggttt cttctaacgg ttctgaagaa tacctggaac tgaacatcat catcgacggt      420

gaaatctcta aatctggtct ggacaaactg gaagaagact acggttcttc tatcaccatc      480

ccgaacgaca aaaacatgat catcaccttc ctgtctaaca aatctgacaa ctaa            534


<210> 40
<211> 177
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 40
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Lys Asn 
        35                  40                  45              


Ile Glu Lys Ala Lys Ile Ala Lys Leu Glu Ala Asp Ile Ser Ala Ile 
    50                  55                  60                  


Lys Ser Ala Ser Leu Ser Tyr Tyr Ala Asp Glu Ser Lys Tyr Thr Glu 
65                  70                  75                  80  


Gly Asn Ile Ile Trp Trp Thr Lys Lys Asp Gly Lys Ile Thr Val Asn 
                85                  90                  95      


Ser Gly Ile Gly Asp Glu Asp Pro Leu Ala His Lys Ile Glu Asn Leu 
            100                 105                 110         


Gly Met Pro Tyr Asn Gly Ser Tyr Thr Leu Val Ser Ser Asn Gly Ser 
        115                 120                 125             


Glu Glu Tyr Leu Glu Leu Asn Ile Ile Ile Asp Gly Glu Ile Ser Lys 
    130                 135                 140                 


Ser Gly Leu Asp Lys Leu Glu Glu Asp Tyr Gly Ser Ser Ile Thr Ile 
145                 150                 155                 160 


Pro Asn Asp Lys Asn Met Ile Ile Thr Phe Leu Ser Asn Lys Ser Asp 
                165                 170                 175     


Asn 
    


<210> 41
<211> 819
<212> DNA
<213> Clostridium difficile

<400> 41
atgggaatga ttattatgaa taaaaagggt tttacattaa ttgaattgtt ggtagttata       60

tctataatag gaattttagt tatagtagct gttccagcgt tatttagaaa tatagaaaaa      120

agtaaggcag ttacatgtct ttctaataga gaaaatataa agactcaaat tgttattgca      180

atggctgagg aatcaagtaa agacaagaat gaagtcataa aagaggtatt agaaaacaaa      240

gatggtaagt actttgaaac agaaccaaag tgtaagtcag gtggaatata ttcagcaacg      300

tttgatgatg gttatgatgg aataactgga atagaaagca ttgcaaaagt gtatgttact      360

tgtacaaaac atccagatgg tattgaaatg gctagggata tacatcaaag tatgaaagat      420

ttgattgcat catttgcaca agacccttct ataataccag gagcttcaaa gggcaatgat      480

gattttagaa aatatttatt agacaataaa tataaaaatg ggtggcctac aattccagat      540

gaatttaagg caaaatatgg attaagtaag gatacactat atatacaacc atatgcatat      600

aatcctacta aatctgatgc tactgtagtt gtatttgcaa ataataagac tggaggtaat      660

tggtatactt ccctagttta cgattatgat gaaggtagat ggtataaagg taaaaatggt      720

atttctgttg caggtaggtc atgggatgtt gacacagata gtgttaagtc tgtaaaaaca      780

gagattcatt ctaaagaggg atggggtcct ttaaattaa                             819


<210> 42
<211> 272
<212> PRT
<213> Clostridium difficile

<400> 42
Met Gly Met Ile Ile Met Asn Lys Lys Gly Phe Thr Leu Ile Glu Leu 
1               5                   10                  15      


Leu Val Val Ile Ser Ile Ile Gly Ile Leu Val Ile Val Ala Val Pro 
            20                  25                  30          


Ala Leu Phe Arg Asn Ile Glu Lys Ser Lys Ala Val Thr Cys Leu Ser 
        35                  40                  45              


Asn Arg Glu Asn Ile Lys Thr Gln Ile Val Ile Ala Met Ala Glu Glu 
    50                  55                  60                  


Ser Ser Lys Asp Lys Asn Glu Val Ile Lys Glu Val Leu Glu Asn Lys 
65                  70                  75                  80  


Asp Gly Lys Tyr Phe Glu Thr Glu Pro Lys Cys Lys Ser Gly Gly Ile 
                85                  90                  95      


Tyr Ser Ala Thr Phe Asp Asp Gly Tyr Asp Gly Ile Thr Gly Ile Glu 
            100                 105                 110         


Ser Ile Ala Lys Val Tyr Val Thr Cys Thr Lys His Pro Asp Gly Ile 
        115                 120                 125             


Glu Met Ala Arg Asp Ile His Gln Ser Met Lys Asp Leu Ile Ala Ser 
    130                 135                 140                 


Phe Ala Gln Asp Pro Ser Ile Ile Pro Gly Ala Ser Lys Gly Asn Asp 
145                 150                 155                 160 


Asp Phe Arg Lys Tyr Leu Leu Asp Asn Lys Tyr Lys Asn Gly Trp Pro 
                165                 170                 175     


Thr Ile Pro Asp Glu Phe Lys Ala Lys Tyr Gly Leu Ser Lys Asp Thr 
            180                 185                 190         


Leu Tyr Ile Gln Pro Tyr Ala Tyr Asn Pro Thr Lys Ser Asp Ala Thr 
        195                 200                 205             


Val Val Val Phe Ala Asn Asn Lys Thr Gly Gly Asn Trp Tyr Thr Ser 
    210                 215                 220                 


Leu Val Tyr Asp Tyr Asp Glu Gly Arg Trp Tyr Lys Gly Lys Asn Gly 
225                 230                 235                 240 


Ile Ser Val Ala Gly Arg Ser Trp Asp Val Asp Thr Asp Ser Val Lys 
                245                 250                 255     


Ser Val Lys Thr Glu Ile His Ser Lys Glu Gly Trp Gly Pro Leu Asn 
            260                 265                 270         


<210> 43
<211> 852
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 43
atgcaccatc atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa       60

accgctgctg ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac      120

gacgacaagg ccatgggtcg taacatcgaa aaatctaaag ctgttacctg cctgtctaac      180

cgtgaaaaca tcaaaaccca gatcgttatc gctatggctg aagaatcttc taaagacaaa      240

aacgaagtta tcaaagaagt tctggaaaac aaagacggta aatacttcga aaccgaaccg      300

aaatgcaaat ctggtggtat ctactctgct accttcgacg acggttacga cggtatcacc      360

ggtatcgaat ctatcgctaa agtttacgtt acctgcacca aacacccgga cggtatcgaa      420

atggctcgtg acatccacca gtctatgaaa gacctgatcg cttctttcgc tcaggacccg      480

tctatcatcc cgggtgcttc taaaggtaac gacgacttcc gtaaatacct gctggacaac      540

aaatacaaaa acggttggcc gaccatcccg gacgaattca aagctaaata cggtctgtct      600

aaagacaccc tgtacatcca gccgtacgct tacaacccga ccaaatctga cgctaccgtt      660

gttgttttcg ctaacaacaa aaccggtggt aactggtaca cctctctggt ttacgactac      720

gacgaaggtc gttggtacaa aggtaaaaac ggtatctctg ttgctggtcg ttcttgggac      780

gttgacaccg actctgttaa atctgttaaa accgaaatcc actctaaaga aggttggggt      840

ccgctgaact aa                                                          852


<210> 44
<211> 283
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 44
Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met Gly Arg Asn 
        35                  40                  45              


Ile Glu Lys Ser Lys Ala Val Thr Cys Leu Ser Asn Arg Glu Asn Ile 
    50                  55                  60                  


Lys Thr Gln Ile Val Ile Ala Met Ala Glu Glu Ser Ser Lys Asp Lys 
65                  70                  75                  80  


Asn Glu Val Ile Lys Glu Val Leu Glu Asn Lys Asp Gly Lys Tyr Phe 
                85                  90                  95      


Glu Thr Glu Pro Lys Cys Lys Ser Gly Gly Ile Tyr Ser Ala Thr Phe 
            100                 105                 110         


Asp Asp Gly Tyr Asp Gly Ile Thr Gly Ile Glu Ser Ile Ala Lys Val 
        115                 120                 125             


Tyr Val Thr Cys Thr Lys His Pro Asp Gly Ile Glu Met Ala Arg Asp 
    130                 135                 140                 


Ile His Gln Ser Met Lys Asp Leu Ile Ala Ser Phe Ala Gln Asp Pro 
145                 150                 155                 160 


Ser Ile Ile Pro Gly Ala Ser Lys Gly Asn Asp Asp Phe Arg Lys Tyr 
                165                 170                 175     


Leu Leu Asp Asn Lys Tyr Lys Asn Gly Trp Pro Thr Ile Pro Asp Glu 
            180                 185                 190         


Phe Lys Ala Lys Tyr Gly Leu Ser Lys Asp Thr Leu Tyr Ile Gln Pro 
        195                 200                 205             


Tyr Ala Tyr Asn Pro Thr Lys Ser Asp Ala Thr Val Val Val Phe Ala 
    210                 215                 220                 


Asn Asn Lys Thr Gly Gly Asn Trp Tyr Thr Ser Leu Val Tyr Asp Tyr 
225                 230                 235                 240 


Asp Glu Gly Arg Trp Tyr Lys Gly Lys Asn Gly Ile Ser Val Ala Gly 
                245                 250                 255     


Arg Ser Trp Asp Val Asp Thr Asp Ser Val Lys Ser Val Lys Thr Glu 
            260                 265                 270         


Ile His Ser Lys Glu Gly Trp Gly Pro Leu Asn 
        275                 280             


