                         SEQUENCE LISTING

<110>  Firmenich SA
 
<120>  METHOD FOR PRODUCING (+)-ZIZAENE

<130>  7640 PCT zizaene synthase

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  555
<212>  PRT
<213>  Vetiveria zizanoides

<400>  1

Met Ala Thr Thr Ala Ala Phe Cys Leu Thr Thr Thr Pro Ile Gly Glu 
1               5                   10                  15      


Pro Val Cys Arg Arg Gln Tyr Leu Pro Thr Val Trp Gly Ser Phe Phe 
            20                  25                  30          


Leu Thr Tyr Gln Pro Cys Thr Pro Glu Glu Val Gln Ser Met Glu Glu 
        35                  40                  45              


Arg Ala Leu Ala Lys Lys Thr Glu Val Gly Arg Met Leu Gln Glu Val 
    50                  55                  60                  


Ala Ala Ser Ser Asn Leu Ala Arg Lys Leu Gly Leu Val Asp Glu Leu 
65                  70                  75                  80  


Glu Arg Leu Gly Val Asp Tyr His Tyr Lys Thr Glu Ile Asn Asp Leu 
                85                  90                  95      


Leu Gly Ala Ile Tyr Asn Gly Lys Asp Asp Asp Asn Gly Gly Ser Asp 
            100                 105                 110         


Asp Asp Leu Tyr Ile Thr Ser Leu Lys Phe Tyr Leu Leu Arg Lys His 
        115                 120                 125             


Gly Tyr Ala Leu Ser Ser Asp Val Phe Leu Lys Phe Arg Asp Glu Gln 
    130                 135                 140                 


Gly Asn Ile Ser Ser Asp Asp Val Lys Cys Leu Ile Met Leu Tyr Asp 
145                 150                 155                 160 


Ala Ser His Leu Arg Ile His Glu Glu Lys Ile Leu Asp Asn Ile Asn 
                165                 170                 175     


Ser Phe Thr Lys Ser Cys Leu Gln Ser Val Leu Glu Thr Asn Leu Glu 
            180                 185                 190         


Pro Ala Leu Gln Glu Glu Val Arg Cys Thr Leu Glu Thr Pro Arg Phe 
        195                 200                 205             


Arg Arg Val Glu Arg Ile Glu Ala Lys Arg Phe Ile Ser Ala Tyr Glu 
    210                 215                 220                 


Lys Asn Ile Ala Arg Asp Asp Ala Leu Leu Glu Phe Ala Arg Leu Asp 
225                 230                 235                 240 


Tyr Asn Ile Val Gln Ile Leu Tyr Cys Lys Glu Leu Lys Glu Leu Thr 
                245                 250                 255     


Val Trp Trp Lys Glu Phe His Ser Arg Thr Asn Leu Thr Phe Ala Arg 
            260                 265                 270         


Asp Arg Ile Val Glu Met Tyr Phe Trp Val Met Ala Ile Ile Tyr Glu 
        275                 280                 285             


Pro Cys Tyr Ser Tyr Ser Arg Ile Trp Val Thr Lys Met Phe Leu Ser 
    290                 295                 300                 


Val Ala Leu Leu Asp Asp Ile Tyr Asp Asn Tyr Thr Ser Thr Glu Glu 
305                 310                 315                 320 


Ser Asn Ile Phe Thr Thr Ala Met Glu Arg Trp Asp Val Lys Ala Thr 
                325                 330                 335     


Glu Gln Leu Pro Ala Asn Met Arg Thr Phe Tyr Asp Tyr Leu Ile Cys 
            340                 345                 350         


Thr Thr Asp Glu Val Val Glu Glu Leu Lys Leu Gln Asn Asn Lys Asn 
        355                 360                 365             


Ala Glu Leu Val Lys Lys Val Leu Ile Asp Ala Ala Lys Cys Tyr His 
    370                 375                 380                 


Ser Glu Val Lys Trp Arg Asp Asp His Tyr Val Pro Asn Asp Val Gly 
385                 390                 395                 400 


Glu His Leu Gln Leu Ser Met Arg Ser Ile Ala Ala Met His Ser Ile 
                405                 410                 415     


Asn Phe Val Phe Ile Ser Leu Gly Ala Val Cys Thr Arg Glu Ala Val 
            420                 425                 430         


Glu Cys Ala Phe Thr Tyr Pro Lys Ile Ile Arg Gly Ile Cys Val His 
        435                 440                 445             


Ala Arg Ile Ser Asn Asp Ile Ala Ser His Glu Arg Glu Gln Ala Ser 
    450                 455                 460                 


Glu His Met Ala Ser Thr Leu Gln Thr Cys Met Lys Gln Tyr Gly Ile 
465                 470                 475                 480 


Thr Val Glu Glu Ala Ala Glu Lys Leu Arg Val Ile Asn Glu Glu Ser 
                485                 490                 495     


Trp Met Asp Ile Val Glu Glu Cys Leu Tyr Lys Asp Gln Tyr Pro Leu 
            500                 505                 510         


Ala Leu Ser Glu Arg Val Val Ala Phe Ala Gln Ser Ile Cys Phe Met 
        515                 520                 525             


Tyr Asn Gly Val Asp Lys Tyr Thr Ile Pro Ser Lys Leu Lys Asp Ser 
    530                 535                 540                 


Leu Asp Ser Leu Tyr Val Asn Leu Ile Pro Val 
545                 550                 555 


<210>  2
<211>  1668
<212>  DNA
<213>  Vetiveria zizanoides

<400>  2
atggcgacga ctgccgcctt ctgcctcacc accactccga tcggcgagcc agtctgtcgc       60

cggcagtacc tcccaaccgt ctggggcagc ttcttcctca cctaccagcc atgcacgccg      120

gaagaggtcc agtccatgga ggagagggct ctggccaaga agacggaggt ggggcgcatg      180

ttgcaggagg tcgccgcctc cagtaacctc gcacggaagc tgggccttgt cgatgagcta      240

gagcggctcg gggtggacta tcactacaag acggagatca acgacttgct gggtgccatt      300

tataatggca aggacgacga taatggaggt tctgatgacg acctctatat cacatcgctt      360

aagttctatc tgctcaggaa gcatgggtac gctttatctt cagatgtgtt tctgaagttc      420

agagatgagc aaggaaatat ttcaagtgat gatgtgaaat gcctgatcat gttgtatgat      480

gcctcacatt tgaggattca tgaggagaaa attcttgaca acatcaacag tttcaccaag      540

agctgcctcc aatcagtttt agaaacaaat ttggaaccgg ctctccaaga ggaggtgcgg      600

tgcacattgg agacacctcg attcagaagg gttgagagaa tcgaagcgaa acgctttatc      660

tcagcgtacg aaaagaacat agcacgagat gacgccctac tagagtttgc aaggctggac      720

tacaatatcg tgcaaattct ctactgcaag gagctgaaag aacttacagt atggtggaag      780

gagttccatt cacggacaaa tctgacattt gcacgagata gaattgtgga gatgtatttc      840

tgggtcatgg caattattta cgagccttgt tactcgtatt cacggatatg ggttacaaaa      900

atgtttctat ccgtggcatt gttggatgac atctatgaca attatacgag cacagaggag      960

agcaatatct ttactacggc catggaaagg tgggatgtga aggccaccga acaactgcca     1020

gcaaacatga ggacattcta cgattactta atttgtacaa cagatgaggt cgtagaagaa     1080

ttgaaacttc agaataataa gaatgctgaa ttagtcaaga aagtgctgat tgacgccgct     1140

aaatgctacc attcggaggt caaatggcgt gatgaccact acgtccctaa tgatgttgga     1200

gagcacctgc agctttcaat gcgaagcatt gcagctatgc actccatcaa ctttgtcttc     1260

atttcactgg gagctgtgtg tactagggag gcggttgagt gtgctttcac ttatccaaaa     1320

attattagag gtatatgtgt tcacgcacgt attagtaacg atatcgcgtc acatgagcga     1380

gaacaagctt cggagcatat ggcatcaacg ttgcaaactt gcatgaagca gtatgggatt     1440

acagtagagg aagctgctga aaagctcaga gtaataaacg aggagtcatg gatggacatc     1500

gttgaggaat gcctttataa ggaccagtat cccctggcgc tttcggagag ggtggtggct     1560

tttgcacaat caatatgttt catgtacaat ggtgtagata aatacaccat accatcaaaa     1620

ctcaaggaca gtctagactc attgtacgtc aatttgattc cagtttga                  1668


<210>  3
<211>  1090
<212>  DNA
<213>  Vetiveria zizanoides

<400>  3
tgtgaaatgc ctgatcatgt tgtatgatgc ctcacatttg aggattcatg aggagaaaat       60

tcttgacaac atcaacagtt tcaccaagag ctgcctccaa tcagttttag aaacaaattt      120

ggaaccggct ctccaagagg aggtgcggtg cacattggag acacctcgat tcagaagggt      180

tgagagaatc gaagcgaaac gctttatctc agcgtacgaa aagaacatag cacgagatga      240

cgccctacta gagtttgcaa ggctggacta caatatcgtg caaattctct actgcaagga      300

gctgaaagaa cttacagtat ggtggaagga gttccattca cggacaaatc tgacatttgc      360

acgagataga attgtggaga tgtatttctg ggtcatggca attatttacg agccttgtta      420

ctcgtattca cggatatggg ttacaaaaat gtttctatcc gtggcattgt tggatgacat      480

ctatgacaat tatacgagca cagaggagag caatatcttt actacggcca tggaaaggtg      540

ggatgtgaag gccaccgaac aactgccagc aaacatgagg acattctacg attacttaat      600

ttgtacaaca gatgaggtcg tagaagaatt gaaacttcag aataataaga atgctgaatt      660

agtcaagaaa gtgctgattg acgccgctaa atgctaccat tcggaggtca aatggcgtga      720

tgaccactac gtccctaatg atgttggaga gcacctgcag ctttcaatgc gaagcattgc      780

agctatgcac tccatcaact ttgtcttcat ttcactggga gctgtgtgta ctagggaggc      840

ggttgagtgt gctttcactt atccaaaaat tattagaggt atatgtgttc acgcacgtat      900

tagtaacgat atcgcgtcac atgagcgaga acaagcttcg gagcatatgg catcaacgtt      960

gcaaacttgc atgaagcagt atgggattac agtagaggaa gctgctgaaa agctcagagt     1020

aataaacgag gagtcatgga tggacatcgt tgaggaatgc ctttataagg accagtatcc     1080

cctggcgctt                                                            1090


<210>  4
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  4
catagcacga gatgacgccc tactagagt                                         29


<210>  5
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  5
gcaaggctgg actacaatat cgtgca                                            26


<210>  6
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  6
cggtggcctt cacatcccac ctttccat                                          28


<210>  7
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  7
aagatattgc tctcctctgt gctcgtat                                          28


<210>  8
<211>  1925
<212>  DNA
<213>  Vetiveria zizanoides

<400>  8
gaagcaaagc catctgccgt gctatcactc tagcaaatta tactgagtgg ataaacttaa       60

taccacacca gacgttttgc attcatggcg acgactgccg ccttctgcct caccaccact      120

ccgatcggcg agccagtctg tcgccggcag tacctcccaa ccgtctgggg cagcttcttc      180

ctcacctacc agccatgcac gccggaagag gtccagtcca tggaggagag ggctctggcc      240

aagaagacgg aggtggggcg catgttgcag gaggtcgccg cctccagtaa cctcgcacgg      300

aagctgggcc ttgtcgatga gctagagcgg ctcggggtgg actatcacta caagacggag      360

atcaacgact tgctgggtgc catttataat ggcaaggacg acgataatgg aggttctgat      420

gacgacctct atatcacatc gcttaagttc tatctgctca ggaagcatgg gtacgcttta      480

tcttcagatg tgtttctgaa gttcagagat gagcaaggaa atatttcaag tgatgatgtg      540

aaatgcctga tcatgttgta tgatgcctca catttgagga ttcatgagga gaaaattctt      600

gacaacatca acagtttcac caagagctgc ctccaatcag ttttagaaac aaatttggaa      660

ccggctctcc aagaggaggt gcggtgcaca ttggagacac ctcgattcag aagggttgag      720

agaatcgaag cgaaacgctt tatctcagcg tacgaaaaga acatagcacg agatgacgcc      780

ctactagagt ttgcaaggct ggactacaat atcgtgcaaa ttctctactg caaggagctg      840

aaagaactta cagtatggtg gaaggagttc cattcacgga caaatctgac atttgcacga      900

gatagaattg tggagatgta tttctgggtc atggcaatta tttacgagcc ttgttactcg      960

tattcacgga tatgggttac aaaaatgttt ctatccgtgg cattgttgga tgacatctat     1020

gacaattata cgagcacaga ggagagcaat atctttacta cggccatgga aaggtgggat     1080

gtgaaggcca ccgaacaact gccagcaaac atgaggacat tctacgatta cttaatttgt     1140

acaacagatg aggtcgtaga agaattgaaa cttcagaata ataagaatgc tgaattagtc     1200

aagaaagtgc tgattgacgc cgctaaatgc taccattcgg aggtcaaatg gcgtgatgac     1260

cactacgtcc ctaatgatgt tggagagcac ctgcagcttt caatgcgaag cattgcagct     1320

atgcactcca tcaactttgt cttcatttca ctgggagctg tgtgtactag ggaggcggtt     1380

gagtgtgctt tcacttatcc aaaaattatt agaggtatat gtgttcacgc acgtattagt     1440

aacgatatcg cgtcacatga gcgagaacaa gcttcggagc atatggcatc aacgttgcaa     1500

acttgcatga agcagtatgg gattacagta gaggaagctg ctgaaaagct cagagtaata     1560

aacgaggagt catggatgga catcgttgag gaatgccttt ataaggacca gtatcccctg     1620

gcgctttcgg agagggtggt ggcttttgca caatcaatat gtttcatgta caatggtgta     1680

gataaataca ccataccatc aaaactcaag gacagtctag actcattgta cgtcaatttg     1740

attccagttt gacgacatcg catcaagtat taattctagg cttaatataa tgccagtaaa     1800

catcatatgt aagggatatt tactttcgtg aatccaaata atttgagggg tcctgtgttc     1860

ctcttaccaa ggatatgtca tcaagttgaa aaatatagcc agcaaaaaaa aaaaaaaaaa     1920

aaaaa                                                                 1925


<210>  9
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  9
caccatggcg acgactgccg ccttct                                            26


<210>  10
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  10
cgatgtcgtc aaactggaat ca                                                22


<210>  11
<211>  1671
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Redesigned sequence for E. coli obtained from SEQ ID NO:8

<400>  11
atggctacta cggctgcttt ttgtctgact actactccta ttggtgagcc ggtgtgtcgt       60

cgccaatacc tgccgactgt gtggggtagc ttcttcttga cctaccaacc gtgcaccccg      120

gaagaggtgc aaagcatgga ggagcgtgca ttggccaaaa agaccgaggt tggccgtatg      180

ttgcaagagg tggcggccag cagcaacctg gcccgcaagc tgggtttggt tgacgagctg      240

gagcgtctgg gtgtggacta ccattacaaa accgagatta acgatctgct gggcgcgatc      300

tataatggta aggacgatga caacggcggt agcgacgatg atctgtacat tacgtctctg      360

aaattctatc tgctgcgtaa gcatggttat gcattgagca gcgatgtttt tctgaaattt      420

cgcgacgagc agggtaacat tagctccgac gacgtcaagt gcctgatcat gttgtacgac      480

gcgagccact tgcgtattca tgaggagaaa atcctggata atatcaattc cttcacgaag      540

agctgcctgc aaagcgttct ggaaaccaat ctggaaccgg cgctgcagga agaggttcgc      600

tgtacgctgg agacgccgcg tttccgccgt gtcgaacgta ttgaagcaaa acgcttcatt      660

agcgcgtacg agaagaacat tgcgcgtgac gacgctctgc tggagttcgc gcgcctggac      720

tacaacattg tccagattct gtattgcaaa gagctgaaag aactgacggt gtggtggaag      780

gagttccaca gccgcaccaa tctgacgttt gcacgtgatc gcatcgtgga aatgtacttt      840

tgggtcatgg caattatcta cgagccttgc tactcgtata gccgcatttg ggtcaccaaa      900

atgtttttga gcgtcgcact gctggatgat atttacgaca actatactag cacggaggaa      960

agcaacattt tcaccacggc gatggagcgc tgggacgtga aagcgaccga acaactgccg     1020

gcgaatatgc gtacctttta tgactatctg atctgcacca ccgacgaagt tgttgaagaa     1080

ctgaaactgc agaataacaa gaacgcggaa ctggttaaga aggtgctgat cgacgcggcc     1140

aaatgctatc atagcgaagt taaatggcgc gacgatcact acgttccgaa tgacgttggt     1200

gaacatctgc agctgtcgat gcgttccatt gcggcgatgc acagcattaa tttcgtgttc     1260

atttccctgg gcgccgtgtg tactcgcgag gcagtcgaat gcgcgtttac ttatccgaag     1320

atcattcgcg gtatttgtgt ccatgcccgt atctccaatg acattgcctc ccatgagcgc     1380

gagcaggcat ccgagcacat ggctagcact ctgcaaacct gcatgaagca gtatggcatt     1440

accgttgagg aggcggcaga aaaactgcgt gtgattaacg aggagagctg gatggatatc     1500

gtcgaggagt gcctgtacaa ggatcagtat ccgctggcat tgtctgagcg cgtcgttgca     1560

tttgcccaga gcatttgttt tatgtacaat ggcgtggaca agtacaccat tccgagcaag     1620

ctgaaggata gcctggatag cttgtatgtc aacctgattc cggtttaatg a              1671


<210>  12
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  12
ctagccatgg cttcagaaaa agaaattagg                                        30


<210>  13
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  13
ccggaattcc tatttgcttc tcttgtaaac tttgttcaag                             40


<210>  14
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  14
aaggagatat acatatgaca aaaaaagttg gtgtcggtca gg                          42


<210>  15
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  15
ctttaccaga ctcgagttac gcctttttca tctgatcctt tgc                         43


<210>  16
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  16
atggctacta cggctgcttt ttgtc                                             25


<210>  17
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  17
tcattaaacc ggaatcaggt tgacatac                                          28


