                         SEQUENCE LISTING

<110>  The Salk Institute for Biological Studies
       Baylor College of Medicine
       Lundblad, Vicki
       Baumgartner, Bridget
 
<120>  MODULATION OF TERMINASE ACTIVITY AT TELOMERES AND DNA 
       DOUBLE-STRAND BREAKS

<130>  027731-001710PC

<150>  US 61/177792
<151>  2009-05-13

<160>  10    

<170>  PatentIn version 3.3

<210>  1
<211>  1006
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Gly Val Pro Ser Phe Phe Arg Trp Leu Ser Arg Lys Tyr Pro Lys 
1               5                   10                  15      


Ile Ile Ser Pro Val Leu Glu Glu Gln Pro Gln Ile Val Asp Gly Val 
            20                  25                  30          


Ile Leu Pro Leu Asp Tyr Ser Ala Ser Asn Pro Asn Gly Glu Leu Asp 
        35                  40                  45              


Asn Leu Tyr Leu Asp Met Asn Gly Ile Val His Pro Cys Ser His Pro 
    50                  55                  60                  


Glu Asn Lys Pro Pro Pro Glu Thr Glu Asp Glu Met Leu Leu Ala Val 
65                  70                  75                  80  


Phe Glu Tyr Thr Asn Arg Val Leu Asn Met Ala Arg Pro Arg Lys Val 
                85                  90                  95      


Leu Val Met Ala Val Asp Gly Val Ala Pro Arg Ala Lys Met Asn Gln 
            100                 105                 110         


Gln Arg Ala Arg Arg Phe Arg Ser Ala Arg Asp Ala Gln Ile Glu Asn 
        115                 120                 125             


Glu Ala Arg Glu Glu Ile Met Arg Gln Arg Glu Glu Val Gly Glu Ile 
    130                 135                 140                 


Ile Asp Asp Ala Val Arg Asn Lys Lys Thr Trp Asp Ser Asn Ala Ile 
145                 150                 155                 160 


Thr Pro Gly Thr Pro Phe Met Asp Lys Leu Ala Ala Ala Leu Arg Tyr 
                165                 170                 175     


Trp Thr Ala Phe Lys Leu Ala Thr Asp Pro Gly Trp Lys Asn Leu Gln 
            180                 185                 190         


Val Ile Ile Ser Asp Ala Thr Val Pro Gly Glu Gly Glu His Lys Ile 
        195                 200                 205             


Met Asn Phe Ile Arg Ser Gln Arg Ala Asp Pro Glu Tyr Asn Pro Asn 
    210                 215                 220                 


Thr Thr His Cys Ile Tyr Gly Leu Asp Ala Asp Leu Ile Phe Leu Gly 
225                 230                 235                 240 


Leu Ala Thr His Glu Pro His Phe Lys Ile Leu Arg Glu Asp Val Phe 
                245                 250                 255     


Ala Gln Asp Asn Arg Lys Arg Asn Asn Leu Lys Asp Thr Ile Asn Met 
            260                 265                 270         


Thr Glu Glu Glu Lys Gln Phe Leu Gln Lys Gln Asn Ser Glu Gln Pro 
        275                 280                 285             


Phe Leu Trp Leu His Ile Asn Val Leu Arg Glu Tyr Leu Ser Ala Glu 
    290                 295                 300                 


Leu Trp Val Pro Gly Leu Pro Phe Thr Phe Asp Leu Glu Arg Ala Ile 
305                 310                 315                 320 


Asp Asp Trp Val Phe Met Cys Phe Phe Cys Gly Asn Asp Phe Leu Pro 
                325                 330                 335     


His Leu Pro Cys Leu Asp Val Arg Glu Asn Ser Ile Asp Ile Leu Leu 
            340                 345                 350         


Asp Ile Trp Lys Val Val Leu Pro Lys Leu Lys Thr Tyr Met Thr Cys 
        355                 360                 365             


Asp Gly Val Leu Asn Leu Pro Ser Val Glu Thr Leu Leu Gln His Leu 
    370                 375                 380                 


Gly Ser Arg Glu Gly Asp Ile Phe Lys Thr Arg His Ile Gln Glu Ala 
385                 390                 395                 400 


Arg Lys Lys Glu Ala Phe Glu Arg Arg Lys Ala Gln Lys Asn Met Ser 
                405                 410                 415     


Lys Gly Gln Asp Arg His Pro Thr Val Ala Thr Glu Gln Leu Gln Met 
            420                 425                 430         


Tyr Asp Thr Gln Gly Asn Leu Ala Lys Gly Ser Trp Asn Leu Thr Thr 
        435                 440                 445             


Ser Asp Met Val Arg Leu Lys Lys Glu Leu Met Leu Ala Asn Glu Gly 
    450                 455                 460                 


Asn Glu Glu Ala Ile Ala Lys Val Lys Gln Gln Ser Asp Lys Asn Asn 
465                 470                 475                 480 


Glu Leu Met Lys Asp Ile Ser Lys Glu Glu Ile Asp Asp Ala Val Ser 
                485                 490                 495     


Lys Ala Asn Lys Thr Asn Phe Asn Leu Ala Glu Val Met Lys Gln Lys 
            500                 505                 510         


Ile Ile Asn Lys Lys His Arg Leu Glu Lys Asp Asn Glu Glu Glu Glu 
        515                 520                 525             


Ile Ala Lys Asp Ser Lys Lys Val Lys Thr Glu Lys Ala Glu Ser Glu 
    530                 535                 540                 


Cys Asp Leu Asp Ala Glu Ile Lys Asp Glu Ile Val Ala Asp Val Asn 
545                 550                 555                 560 


Asp Arg Glu Asn Ser Glu Thr Thr Glu Val Ser Arg Asp Ser Pro Val 
                565                 570                 575     


His Ser Thr Val Asn Val Ser Glu Gly Pro Lys Asn Gly Val Phe Asp 
            580                 585                 590         


Thr Asp Glu Phe Val Lys Leu Phe Glu Pro Gly Tyr His Glu Arg Tyr 
        595                 600                 605             


Tyr Thr Ala Lys Phe His Val Thr Pro Gln Asp Ile Glu Gln Leu Arg 
    610                 615                 620                 


Lys Asp Met Val Lys Cys Tyr Ile Glu Gly Val Ala Trp Val Leu Met 
625                 630                 635                 640 


Tyr Tyr Tyr Gln Gly Cys Ala Ser Trp Asn Trp Phe Tyr Pro Tyr His 
                645                 650                 655     


Tyr Ala Pro Leu Ala Thr Asp Phe His Gly Phe Ser His Leu Glu Ile 
            660                 665                 670         


Lys Phe Glu Glu Gly Thr Pro Phe Leu Pro Tyr Glu Gln Leu Met Ser 
        675                 680                 685             


Val Leu Pro Ala Ala Ser Gly His Ala Leu Pro Lys Ile Phe Arg Ser 
    690                 695                 700                 


Leu Met Ser Glu Pro Asp Ser Glu Ile Ile Asp Phe Tyr Pro Glu Glu 
705                 710                 715                 720 


Phe Pro Ile Asp Met Asn Gly Lys Lys Met Ser Trp Gln Gly Ile Ala 
                725                 730                 735     


Leu Leu Pro Phe Ile Asp Gln Asp Arg Leu Leu Thr Ala Val Arg Ala 
            740                 745                 750         


Gln Tyr Pro Leu Leu Ser Asp Ala Glu Arg Ala Arg Asn Ile Arg Gly 
        755                 760                 765             


Glu Pro Val Leu Leu Ile Ser Asn Lys Asn Ala Asn Tyr Glu Arg Phe 
    770                 775                 780                 


Ser Lys Lys Leu Tyr Ser Lys Glu Asn Asn Asn Asn Asn Val Val Val 
785                 790                 795                 800 


Lys Phe Gln His Phe Lys Ser Gly Leu Ser Gly Ile Val Ser Lys Asp 
                805                 810                 815     


Val Glu Gly Phe Glu Leu Asn Gly Lys Ile Val Cys Pro Ile Gln Gly 
            820                 825                 830         


Gly Ser Leu Pro Asn Leu Ser Thr Thr Leu Ile Leu Lys Met Ser Tyr 
        835                 840                 845             


Arg Leu Ile Pro Leu Pro Ser Arg Asn Lys Ser Ile Ile Leu Asn Gly 
    850                 855                 860                 


Phe Ile Pro Ser Glu Pro Val Leu Thr Ala Tyr Asp Leu Asp Ser Ile 
865                 870                 875                 880 


Met Tyr Lys Tyr Asn Asn Gln Asn Tyr Ser Arg Arg Trp Asn Phe Gly 
                885                 890                 895     


Asn Asp Leu Lys Gln Asn Ile Val Pro Val Gly Pro Lys Gly Ile Thr 
            900                 905                 910         


Gln Tyr Lys Pro Arg Thr Gly Gly Tyr Arg Ala Phe Phe Tyr Phe Ala 
        915                 920                 925             


Glu Leu Ser Arg Asn Asn Val Gln Pro Ala His Asn Tyr Gly Arg Asn 
    930                 935                 940                 


Ser Tyr Asn Ser Gln Pro Gly Phe Asn Asn Ser Arg Tyr Asp Gly Gly 
945                 950                 955                 960 


Asn Asn Asn Tyr Arg Gln Asn Ser Asn Tyr Arg Asn Asn Asn Tyr Ser 
                965                 970                 975     


Gly Asn Arg Asn Ser Gly Gln Tyr Ser Gly Asn Ser Tyr Ser Arg Asn 
            980                 985                 990         


Asn Lys Gln Ser Arg Tyr Asp Asn  Ser Arg Ala Asn Arg  Arg 
        995                 1000                 1005     


<210>  2
<211>  950
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Val Pro Ala Phe Phe Arg Trp Leu Ser Arg Lys Tyr Pro Ser 
1               5                   10                  15      


Ile Ile Val Asn Cys Val Glu Glu Lys Pro Lys Glu Cys Asn Gly Val 
            20                  25                  30          


Lys Ile Pro Val Asp Ala Ser Lys Pro Asn Pro Asn Asp Val Glu Phe 
        35                  40                  45              


Asp Asn Leu Tyr Leu Asp Met Asn Gly Ile Ile His Pro Cys Thr His 
    50                  55                  60                  


Pro Glu Asp Lys Pro Ala Pro Lys Asn Glu Asp Glu Met Met Val Ala 
65                  70                  75                  80  


Ile Phe Glu Tyr Ile Asp Arg Leu Phe Ser Ile Val Arg Pro Arg Arg 
                85                  90                  95      


Leu Leu Tyr Met Ala Ile Asp Gly Val Ala Pro Arg Ala Lys Met Asn 
            100                 105                 110         


Gln Gln Arg Ser Arg Arg Phe Arg Ala Ser Lys Glu Gly Met Glu Ala 
        115                 120                 125             


Ala Val Glu Lys Gln Arg Val Arg Glu Glu Ile Leu Ala Lys Gly Gly 
    130                 135                 140                 


Phe Leu Pro Pro Glu Glu Ile Lys Glu Arg Phe Asp Ser Asn Cys Ile 
145                 150                 155                 160 


Thr Pro Gly Thr Glu Phe Met Asp Asn Leu Ala Lys Cys Leu Arg Tyr 
                165                 170                 175     


Tyr Ile Ala Asp Arg Leu Asn Asn Asp Pro Gly Trp Lys Asn Leu Thr 
            180                 185                 190         


Val Ile Leu Ser Asp Ala Ser Ala Pro Gly Glu Gly Glu His Lys Ile 
        195                 200                 205             


Met Asp Tyr Ile Arg Arg Gln Arg Ala Gln Pro Asn His Asp Pro Asn 
    210                 215                 220                 


Thr His His Cys Leu Cys Gly Ala Asp Ala Asp Leu Ile Met Leu Gly 
225                 230                 235                 240 


Leu Ala Thr His Glu Pro Asn Phe Thr Ile Ile Arg Glu Glu Phe Lys 
                245                 250                 255     


Pro Asn Lys Pro Lys Pro Cys Gly Leu Cys Asn Gln Phe Gly His Glu 
            260                 265                 270         


Val Lys Asp Cys Glu Gly Leu Pro Arg Glu Lys Lys Gly Lys His Asp 
        275                 280                 285             


Glu Leu Ala Asp Ser Leu Pro Cys Ala Glu Gly Glu Phe Ile Phe Leu 
    290                 295                 300                 


Arg Leu Asn Val Leu Arg Glu Tyr Leu Glu Arg Glu Leu Thr Met Ala 
305                 310                 315                 320 


Ser Leu Pro Phe Thr Phe Asp Val Glu Arg Ser Ile Asp Asp Trp Val 
                325                 330                 335     


Phe Met Cys Phe Phe Val Gly Asn Asp Phe Leu Pro His Leu Pro Ser 
            340                 345                 350         


Leu Glu Ile Arg Glu Asn Ala Ile Asp Arg Leu Val Asn Ile Tyr Lys 
        355                 360                 365             


Asn Val Val His Lys Thr Gly Gly Tyr Leu Thr Glu Ser Gly Tyr Val 
    370                 375                 380                 


Asn Leu Gln Arg Val Gln Met Ile Met Leu Ala Val Gly Glu Val Glu 
385                 390                 395                 400 


Asp Ser Ile Phe Lys Lys Arg Lys Asp Asp Glu Asp Ser Phe Arg Arg 
                405                 410                 415     


Arg Gln Lys Glu Lys Arg Lys Arg Met Lys Arg Asp Gln Pro Ala Phe 
            420                 425                 430         


Thr Pro Ser Gly Ile Leu Thr Pro His Ala Leu Gly Ser Arg Asn Ser 
        435                 440                 445             


Pro Gly Ser Gln Val Ala Ser Asn Pro Arg Gln Ala Ala Tyr Glu Met 
    450                 455                 460                 


Arg Met Gln Asn Asn Ser Ser Pro Ser Ile Ser Pro Asn Thr Ser Phe 
465                 470                 475                 480 


Thr Ser Asp Gly Ser Pro Ser Pro Leu Gly Gly Ile Lys Arg Lys Ala 
                485                 490                 495     


Glu Asp Ser Asp Ser Glu Pro Glu Pro Glu Asp Asn Val Arg Leu Trp 
            500                 505                 510         


Glu Ala Gly Trp Lys Gln Arg Tyr Tyr Lys Asn Lys Phe Asp Val Asp 
        515                 520                 525             


Ala Ala Asp Glu Lys Phe Arg Arg Lys Val Val Gln Ser Tyr Val Glu 
    530                 535                 540                 


Gly Leu Cys Trp Val Leu Arg Tyr Tyr Tyr Gln Gly Cys Ala Ser Trp 
545                 550                 555                 560 


Lys Trp Tyr Tyr Pro Phe His Tyr Ala Pro Phe Ala Ser Asp Phe Glu 
                565                 570                 575     


Gly Ile Ala Asp Met Pro Ser Asp Phe Glu Lys Gly Thr Lys Pro Phe 
            580                 585                 590         


Lys Pro Leu Glu Gln Leu Met Gly Val Phe Pro Ala Ala Ser Gly Asn 
        595                 600                 605             


Phe Leu Pro Pro Ser Trp Arg Lys Leu Met Ser Asp Pro Asp Ser Ser 
    610                 615                 620                 


Ile Ile Asp Phe Tyr Pro Glu Asp Phe Ala Ile Asp Leu Asn Gly Lys 
625                 630                 635                 640 


Lys Tyr Ala Trp Gln Gly Val Ala Leu Leu Pro Phe Val Asp Glu Arg 
                645                 650                 655     


Arg Leu Arg Ala Ala Leu Glu Glu Val Tyr Pro Asp Leu Thr Pro Glu 
            660                 665                 670         


Glu Thr Arg Arg Asn Ser Leu Gly Gly Asp Val Leu Phe Val Gly Lys 
        675                 680                 685             


His His Pro Leu His Asp Phe Ile Leu Glu Leu Tyr Gln Thr Gly Ser 
    690                 695                 700                 


Thr Glu Pro Val Glu Val Pro Pro Glu Leu Cys His Gly Ile Gln Gly 
705                 710                 715                 720 


Lys Phe Ser Leu Asp Glu Glu Ala Ile Leu Pro Asp Gln Ile Val Cys 
                725                 730                 735     


Ser Pro Val Pro Met Leu Arg Asp Leu Thr Gln Asn Thr Val Val Ser 
            740                 745                 750         


Ile Asn Phe Lys Asp Pro Gln Phe Ala Glu Asp Tyr Ile Phe Lys Ala 
        755                 760                 765             


Val Met Leu Pro Gly Ala Arg Lys Pro Ala Ala Val Leu Lys Pro Ser 
    770                 775                 780                 


Asp Trp Glu Lys Ser Ser Asn Gly Arg Gln Trp Lys Pro Gln Leu Gly 
785                 790                 795                 800 


Phe Asn Arg Asp Arg Arg Pro Val His Leu Asp Gln Ala Ala Phe Arg 
                805                 810                 815     


Thr Leu Gly His Val Met Pro Arg Gly Ser Gly Thr Gly Ile Tyr Ser 
            820                 825                 830         


Asn Ala Ala Pro Pro Pro Val Thr Tyr Gln Gly Asn Leu Tyr Arg Pro 
        835                 840                 845             


Leu Leu Arg Gly Gln Ala Gln Ile Pro Lys Leu Met Ser Asn Met Arg 
    850                 855                 860                 


Pro Gln Asp Ser Trp Arg Gly Pro Pro Pro Leu Phe Gln Gln Gln Arg 
865                 870                 875                 880 


Phe Asp Arg Gly Val Gly Ala Glu Pro Leu Leu Pro Trp Asn Arg Met 
                885                 890                 895     


Leu Gln Thr Gln Asn Ala Ala Phe Gln Pro Asn Gln Tyr Gln Met Leu 
            900                 905                 910         


Ala Gly Pro Gly Gly Tyr Pro Pro Arg Arg Asp Asp Arg Gly Gly Arg 
        915                 920                 925             


Gln Gly Tyr Pro Arg Glu Gly Arg Lys Tyr Pro Leu Pro Pro Pro Ser 
    930                 935                 940                 


Gly Arg Tyr Asn Trp Asn 
945                 950 


<210>  3
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA oligonucleotide for detecting specific DNA 
       exonuclease activity

<400>  3
cctctaggtt cctttgttac                                                 20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA oligonucleotide for detecting specific DNA 
       exonuclease activity

<400>  4
gtaacaaagg aacctagagg                                                 20


<210>  5
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide for use as anti-peptide antibody

<400>  5

Tyr Ser Gly Asn Ser Tyr Ser Arg Asn Asn Lys Gln Ser Arg Tyr Asp 
1               5                   10                  15      


Asn Ser Arg Ala Asn Arg Arg 
            20              


<210>  6
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide for use as anti-peptide antibody

<400>  6

Asn Asp Arg Glu Asn Ser Glu Thr Thr Glu Val Ser Arg Asp 
1               5                   10                  


<210>  7
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for MAT alpha locus

<400>  7
agatgtgttt gtacatttgg ccttata                                         27


<210>  8
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for MAT alpha locus

<400>  8
tacccttatc tacttgcctc ttttgtt                                         27


<210>  9
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for PMA1 locus

<400>  9
atgaaccaca gtacatcata ttaagacgta                                      30


<210>  10
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for PMA1 locus

<400>  10
gaagaaattt cgatgcgatt aacc                                            24


