                         SEQUENCE LISTING

<110>  Novozymes Biopharma UK Ltd.
 
<120>  METHOD OF CONTROLLING O-LINKED GLYCOSYLATION OF ANTIBODIES

<130>  11767.204-WO

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Primer LES 21

<400>  1
gttggtcgct gcttcccaag ctgccttagg tttgtaataa gcttaattct tatg           54


<210>  2
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Primer LES 22

<400>  2
cataagaatt aagcttatta caaacctaag gcagcttggg aagcagcgac caac           54


<210>  3
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Fwd rHA single FLAG

<400>  3
ttaggcttag attataaaga tgatgacgat aaataata                             38


<210>  4
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Primer Rev rHA single FLAG

<400>  4
agcttattat ttatcgtcat catctttata atctaagcc                            39


<210>  5
<211>  774
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  5
agatctctgc agaagttcaa ttgttggaat ctggtggtgg tttggttcaa cctggtggtt     60

ctttgagatt gtcttgtgct gcttctggtt ttactttttc taattattgg atgtcttggg    120

ttagacaagc tccaggtaaa ggtttggaat gggtttccgg tatttcaggt aatggtggtt    180

atacttattt tgctgattca gttaaagata gatttactat ttctagagat aattctaaaa    240

ataccttata tttgcaaatg aactctttga gagcagaaga tactgctgtt tattactgtg    300

caggtggtga cggttctggt tggagttttt ggggtcaagg tactctagtt accgtttctt    360

caggtggtgg tggttctggt ggaggtggat caggtggtgg aggatctcaa tcagttttga    420

ctcaaccacc atctgcttca ggtactccag gtcaaagagt taccatttct tgtactggtt    480

cttcttctaa tattggtgca ggttacgatg ttcattggta tcaacaattg ccaggtactg    540

ctccaaaatt gttgatttat ggtaacaaca atagaccatc tggtgtccca gatagatttt    600

ctggttctaa atctggtact tctgcttctt tggctatttc tggtttaaga tcagaagatg    660

aagctgatta ctactgtgct gcttgggatg actctttgtc tggtagagtt ttcggtggtg    720

gtactaaatt gaccgttttg ggtgacgctc acaagtccga agtcgctcat cgat          774


<210>  6
<211>  91
<212>  DNA
<213>  Artificial

<220>
<223>  Primer CF 138

<400>  6
agcttaacct aattctaaca agcaaagatg cttttgcaag ccttcctttt ccttttggct     60

ggttttgcag ccaagatctc tgcagaagac a                                    91


<210>  7
<211>  91
<212>  DNA
<213>  Artificial

<220>
<223>  Primer CF 139

<400>  7
agcttgtctt ctgcagagat cttggctgca aaaccagcca aaaggaaaag gaaggcttgc     60

aaaagcatct ttgcttgtta gaattaggtt a                                    91


<210>  8
<211>  389
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic

<400>  8
gaattcaatc agtaaaaatc aacggttaac gacattacta tatatataat ataggaagca     60

tttaatagaa cagcatcgta atatatgtgt actttgcagt tatgacgcca gatggcagta    120

gtggaagata ttctttattg aaaaatagct tgtcacctta cgtacaatct tgatccggag    180

cttttctttt tttgaagctt taaagataat gctaaatcat ttggcttttt gattgattgt    240

acaggaaaat atacatcgca gggggttgac ttttaccatt tcaccgcaat ggaatcaaac    300

ttgttgaaga gaatgttcac aggcgcatac gctacaatga cccgattctt gctagccttt    360

tctcggtctt gcaaacaacc gccgaattc                                      389


<210>  9
<211>  1328
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic

<400>  9
ccatggtcac tcttaaagag aagctgttag tggcctgtct tgctgtcttt acagcggtca     60

ttagattgca tggcttggca tggcctgaca gcgtggtgtt tgatgaagta catttcggtg    120

ggtttgcctc gcaatacatt agggggactt acttcatgga tgtgcatcct cctcttgcaa    180

agatgttgta tgctggtgtg gcatcgcttg gtgggttcca gggtgatttt gacttcgaaa    240

atattggtga cagctttcca tctacgacgc catacgtgtt gatgagattt ttctctgctt    300

ctttgggggc tcttactgtt attttgatgt acatgacttt acgttattct ggtgttcgta    360

tgtgggttgc tttgatgagc gctatctgct ttgccgttga aaactcgtac gtcactattt    420

ctcgttacat tctgttggac gccccattga tgtttttcat tgcagctgca gtctactctt    480

tcaagaaata cgaaatgtac cctgccaact cgctcaatgc ttacaagtcc ttgcttgcta    540

ctggtattgc tcttggtatg gcatcttcat ccaaatgggt tggtcttttc acggttacat    600

gggtgggtct tttatgtatc tggagactat ggttcatgat tggggatttg actaagtctt    660

ccaagtccat cttcaaagta gcatttgcca aattggcctt cttgttgggt gtgccttttg    720

ccctttatct ggtcttcttt tatatccact tccaatcatt aactttggac ggggatggcg    780

caagcttcat ttctaaattt attgaatccc ataaaaagat gtggcatatc aataaaaatt    840

tggtcgaacc tcatgtttat gaatcacaac caacttcatg gccattcttg ctacgtggta    900

taagttactg gggtgaaaat aacagaaacg tctatctatt aggtaatgcg atcgtatggt    960

gggctgtcac cgctttcatc ggtattttcg gattgattgt tatcactgag ctgttctcgt   1020

ggcagttagg taaaccaatt ttgaaggact ccaaggttgt taacttccac gttcaggtta   1080

ttcactactt attgggtttt gccgtccatt atgctccatc tttcttaatg caacgtcaaa   1140

tgtttttgca tcactactta cctgcttatt atttcggtat tcttgccctt ggccacgcct   1200

tggacataat agtttcttat gttttccgca gcaagagaca aatgggctac gcggtagtga   1260

tcactttcct tgctgcttct gtgtatttct tcaagagctt cagtccaatt atttacggta   1320

caccatgg                                                            1328


<210>  10
<211>  865
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic

<400>  10
aagctttcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg     60

agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat    120

ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca    180

gaatgtgcac tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag    240

agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat    300

agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt    360

ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg    420

caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac    480

tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat    540

tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga    600

aggcaagaga gccccgagag cttacatttt atgttagctg gtggactgac gccagaaaat    660

gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag    720

acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag    780

gttattactg agtagtattt atttaagtat tgtttgtgca cttgcctgca agccttttga    840

aaagcaagca taaaagatca agctt                                          865


<210>  11
<211>  1378
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic

<400>  11
agatctggta tccaaaagaa gttgtttttg atgaggtaca tttcgggaaa tttgcatcgt     60

attacttaga aaggtcttat ttctttgacg ttcatccccc ttttgctaag atgatgattg    120

ccttcattgg ttggttatgt ggctatgatg gttcctttaa gtttgatgag attgggtatt    180

cttatgaaac tcatccagct ccatatatcg cgtaccgttc tttcaacgcg atattgggca    240

cattgactgt accaattatg ttcaacactt tgaaggaact gaatttcagg gctattacat    300

gtgcgtttgc atctctcttg gttgcaatcg atactgcgca tgttacagaa actaggctga    360

ttttactgga tgccatcttg attatttcta ttgctgctac tatgtattgt tacgttcgtt    420

tctacaagtg ccaattgcgt caacctttta catggagttg gtatatttgg ttacacgcta    480

ctggtttgtc tttatccttc gtgatttcca caaaatatgt tggtgttatg acatattccg    540

ctattggttt tgctgctgtg gtcaacttat ggcaattact ggacatcaag gcgggtttgt    600

ccttgaggca gttcatgaga cattttagta aaaggctgaa tggtttagtt ttgattccat    660

ttgtgattta cttgttttgg ttctgggttc atttcaccgt tttgaatact tcaggtcctg    720

gcgacgcaag cttaagccat taccattctt gaagaaatgg attgaaactc aaaaatctat    780

gttcgaacat aacaataaac tatcatcaga gcatccattt gcctctgaac cttacagttg    840

gcccggtagt ttaagtggtg tttcgttctg gaccaacggt gacgaaaaga agcaaatata    900

tttcattggt aacatcattg ggtggtggtt ccaagtcata tcattggctg tttttgttgg    960

cattatcgtg gccgatttaa ttactagaca tcgtggctat tatgccctaa acaagatgac   1020

cagagaaaag ctgtatggcc cattgatgtt tttcttcgtc tcctggtgct gtcattattt   1080

tccattcttt ttaatggcgc gtcaaaagtt tttgcatcat tacttaccag ctcatttaat   1140

cgcgtgctta ttctcaggag cactatggga agtaattttc agtgattgca aatcattgga   1200

tttggagaaa gacgaggata tttcaggtgc atcatatgaa cggaacccta aggtctacgt   1260

taaaccctat accgtcttct tggtgtgtgt ctcctgtgct gttgcgtggt tttttgtata   1320

cttttcacca ctagtgtatg gagatgtcag cttgtcacca tcggaagttg tttctaga     1378


<210>  12
<211>  244
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic

<400>  12

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Asn Gly Gly Tyr Thr Tyr Phe Ala Asp Ser Val 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Gly Asp Gly Ser Gly Trp Ser Phe Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly 
    130                 135                 140                 


Thr Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn 
145                 150                 155                 160 


Ile Gly Ala Gly Tyr Asp Val His Trp Tyr Gln Gln Leu Pro Gly Thr 
                165                 170                 175     


Ala Pro Lys Leu Leu Ile Tyr Gly Asn Asn Asn Arg Pro Ala Gly Val 
            180                 185                 190         


Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala 
        195                 200                 205             


Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala 
    210                 215                 220                 


Trp Asp Asp Ser Leu Ser Gly Arg Val Phe Gly Gly Gly Thr Lys Leu 
225                 230                 235                 240 


Thr Val Leu Gly 
                


