                         SEQUENCE LISTING

<110>  OREGON HEALTH & SCIENCE UNIVERSITY
       Grompe, Markus
       Lan, Hongxiang
 
<120>  METHOD OF EXPANDING HUMAN HEPATOCYTES IN VIVO

<130>  899-77961-13

<150>  US 61/296,774
<151>  2010-01-20

<150>  US 61/174,791
<151>  2009-05-01

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  1
ggcgcggtgg ctcacg                                                       16


<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  2
ttttttgaga cggagtctcg ctc                                               23


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  3
atggatgatt tcgcagcttt                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
tggctttaca ccaacgaaaa                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  5
tacagcggag caactgaaga                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  6
ttgcagcaca gagacaagaa                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
ccgggagagt tttaccacaa                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  8
ccttccctag atgggacaca                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  9
ctgacctcac ctgggacaat                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  10
cctccacagg tttcctggta                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
tttgggacca ctgtctctcc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
ctgaccattc cccaggtcta                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
atggcttctc atcgtctgct                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
gctcctcatt ccttgggatt                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
gtgcctttat cacccatgct                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
tcttggattt gtgggctttc                                                   20


<210>  17
<211>  1802
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (127)..(606)

<400>  17
gggcagctcc accctgggag ggactgtggc ccaggtactg cccgggtgct actttatggg       60

cagcagctca gttgagttag agtctggaag acctcagaag acctcctgtc ctatgaggcc      120

ctcccc atg gct tta gag acg atc tgc cga ccc tct ggg aga aaa tcc         168
       Met Ala Leu Glu Thr Ile Cys Arg Pro Ser Gly Arg Lys Ser            
       1               5                   10                             

agc aag atg caa gcc ttc aga atc tgg gat gtt aac cag aag acc ttc        216
Ser Lys Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe           
15                  20                  25                  30            

tat ctg agg aac aac caa cta gtt gct gga tac ttg caa gga cca aat        264
Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn           
                35                  40                  45                

gtc aat tta gaa gaa aag ata gat gtg gta ccc att gag cct cat gct        312
Val Asn Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His Ala           
            50                  55                  60                    

ctg ttc ttg gga atc cat gga ggg aag atg tgc ctg tcc tgt gtc aag        360
Leu Phe Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val Lys           
        65                  70                  75                        

tct ggt gat gag acc aga ctc cag ctg gag gca gtt aac atc act gac        408
Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp           
    80                  85                  90                            

ctg agc gag aac aga aag cag gac aag cgc ttc gcc ttc atc cgc tca        456
Leu Ser Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser           
95                  100                 105                 110           

gac agc ggc ccc acc acc agt ttt gag tct gcc gcc tgc ccc ggt tgg        504
Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp           
                115                 120                 125               

ttc ctc tgc aca gcg atg gaa gct gac cag ccc gtc agc ctc acc aat        552
Phe Leu Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn           
            130                 135                 140                   

atg cct gac gaa ggc gtc atg gtc acc aaa ttc tac ttc cag gag gac        600
Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp           
        145                 150                 155                       

gag tag tactgcccag gcctgcctgt tcccattctt gcatggcaag gactgcaggg         656
Glu                                                                       
                                                                          

actgccagtc cccctgcccc agggctcccg gctatggggg cactgaggac cagccattga      716

ggggtggacc ctcagaaggc gtcacaagaa cctggtcaca ggactctgcc tcctcttcaa      776

ctgaccagcc tccatgctgc ctccagaatg gtctttctaa tgtgtgaatc agagcacagc      836

agcccctgca caaagccctt ccatgtcgcc tctgcattca ggatcaaacc ccgaccacct      896

gcccaacctg ctctcctctt gccactgcct cttcctccct cattccacct tcccatgccc      956

tggatccatc aggccacttg atgaccccca accaagtggc tcccacaccc tgttttacaa     1016

aaaagaaaag accagtccat gagggaggtt tttaagggtt tgtggaaaat gaaaattagg     1076

atttcatgat tttttttttt cagtccccgt gaaggagagc ccttcatttg gagattatgt     1136

tctttcgggg agaggctgag gacttaaaat attcctgcat ttgtgaaatg atggtgaaag     1196

taagtggtag cttttccctt ctttttcttc tttttttgtg atgtcccaac ttgtaaaaat     1256

taaaagttat ggtactatgt tagccccata attttttttt tccttttaaa acacttccat     1316

aatctggact cctctgtcca ggcactgctg cccagcctcc aagctccatc tccactccag     1376

attttttaca gctgcctgca gtactttacc tcctatcaga agtttctcag ctcccaaggc     1436

tctgagcaaa tgtggctcct gggggttctt tcttcctctg ctgaaggaat aaattgctcc     1496

ttgacattgt agagcttctg gcacttggag acttgtatga aagatggctg tgcctctgcc     1556

tgtctccccc accgggctgg gagctctgca gagcaggaaa catgactcgt atatgtctca     1616

ggtccctgca gggccaagca cctagcctcg ctcttggcag gtactcagcg aatgaatgct     1676

gtatatgttg ggtgcaaagt tccctacttc ctgtgacttc agctctgttt tacaataaaa     1736

tcttgaaaat gcctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1796

aaaaaa                                                                1802


<210>  18
<211>  159
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ala Leu Glu Thr Ile Cys Arg Pro Ser Gly Arg Lys Ser Ser Lys 
1               5                   10                  15      


Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe Tyr Leu 
            20                  25                  30          


Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn Val Asn 
        35                  40                  45              


Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His Ala Leu Phe 
    50                  55                  60                  


Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly 
65                  70                  75                  80  


Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp Leu Ser 
                85                  90                  95      


Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser Asp Ser 
            100                 105                 110         


Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu 
        115                 120                 125             


Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn Met Pro 
    130                 135                 140                 


Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150                 155                 


<210>  19
<211>  2316
<212>  DNA
<213>  Mus musculus


<220>
<221>  CDS
<222>  (36)..(572)

<400>  19
gtcgctagtc tctattgcct tgctgtggcc tcggg atg gaa atc tgc tgg gga          53
                                       Met Glu Ile Cys Trp Gly            
                                       1               5                  

ccc tac agt cac cta atc tct ctc ctt ctc atc ctt ctg ttt cat tca        101
Pro Tyr Ser His Leu Ile Ser Leu Leu Leu Ile Leu Leu Phe His Ser           
            10                  15                  20                    

gag gca gcc tgc cgc cct tct ggg aaa aga ccc tgc aag atg caa gcc        149
Glu Ala Ala Cys Arg Pro Ser Gly Lys Arg Pro Cys Lys Met Gln Ala           
        25                  30                  35                        

ttc aga atc tgg gat act aac cag aag acc ttt tac ctg aga aac aac        197
Phe Arg Ile Trp Asp Thr Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn           
    40                  45                  50                            

cag ctc att gct ggg tac tta caa gga cca aat atc aaa cta gaa gaa        245
Gln Leu Ile Ala Gly Tyr Leu Gln Gly Pro Asn Ile Lys Leu Glu Glu           
55                  60                  65                  70            

aag ata gac atg gtg cct att gac ctt cat agt gtg ttc ttg ggc atc        293
Lys Ile Asp Met Val Pro Ile Asp Leu His Ser Val Phe Leu Gly Ile           
                75                  80                  85                

cac ggg ggc aag ctg tgc ctg tct tgt gcc aag tct gga gat gat atc        341
His Gly Gly Lys Leu Cys Leu Ser Cys Ala Lys Ser Gly Asp Asp Ile           
            90                  95                  100                   

aag ctc cag ctg gag gaa gtt aac atc act gat ctg agc aag aac aaa        389
Lys Leu Gln Leu Glu Glu Val Asn Ile Thr Asp Leu Ser Lys Asn Lys           
        105                 110                 115                       

gaa gaa gac aag cgc ttt acc ttc atc cgc tct gag aaa ggc ccc acc        437
Glu Glu Asp Lys Arg Phe Thr Phe Ile Arg Ser Glu Lys Gly Pro Thr           
    120                 125                 130                           

acc agc ttt gag tca gct gcc tgt cca gga tgg ttc ctc tgc aca aca        485
Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Thr           
135                 140                 145                 150           

cta gag gct gac cgt cct gtg agc ctc acc aac aca ccg gaa gag ccc        533
Leu Glu Ala Asp Arg Pro Val Ser Leu Thr Asn Thr Pro Glu Glu Pro           
                155                 160                 165               

ctt ata gtc acg aag ttc tac ttc cag gaa gac caa tag tactgccgag         582
Leu Ile Val Thr Lys Phe Tyr Phe Gln Glu Asp Gln                           
            170                 175                                       

gcctgtaata atcaccaact gcctgatcac tctggccatc attggggcct gaggaacaac      642

ttttgcaggg tgtatgtaca gtagaaggag acagaagagt tctgatgata gatctctgcc      702

tcagtctgtt ggctggccta atccccatga tgattccaga ataatcttgc aaattggatc      762

atggcaggtg cttgttcaaa gccctttctt gttgcctctg ccatctgggt gaagtctaga      822

ccacttgctt ggcctaggtg tcttctgctc taccacccac cctacccctg ccacaaacac      882

acactttttt tgtttttgtt ttttccattg ttctgcactt ccacagtcca gaccaatcaa      942

gtcacttgac aatatgcccc aagtgactcc cttaccctgt tttataaacc tgtgcctgtc     1002

tatggagaag gttttaattc tccttgttat tcattttggg ctttttgatg aaaccaccag     1062

ggcatcacat atactaagca tgtgctctac catcatgcta tgcttccagc tcaggggggc     1122

acttttaagg atctagaaaa cagaaattaa ggatctcata gttattttat taggccagcc     1182

ttattccatg tcggcaagag gtttcttgtg gaaattatgt cctttctgag aggagctggg     1242

gattagatgc tcctgcattt gtgaaatggt tataagcata gaaaaatagg tggtaagctt     1302

tccttctttc cttattttgt gtgatgcctt aaactgaaaa gttaaaaatt gatggattgt     1362

agcattccca taatctcccc cttctttttt tttcctttgg aaatgtccaa tagtctatat     1422

tcctctgtcc cgcccaaaca ccatcttcac tccaagccta ccacagatgc ctgaagaagt     1482

tcctcactat ctgcaaatgt ggctctcagg cccttcctga tgtgatgaat gaatctacta     1542

atcatttctt gaccattcat tttatcactt ctaaccttga aacatgtgga agtagctatg     1602

ttcctgactg tttcctctgc cagacaatga actctggaga tcagggagct tcgtgtgtgt     1662

gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcgtgc gcgcgcgcgt gcgcacgcac     1722

gtgcatgcac atgctatgta ttgggtccct ccaaggatga accctctctt tggcttagaa     1782

ggcactcaga gaatatgtgt tattcgtgct cacggaaagt ttcttattca tccctgtgac     1842

tttggcttta ttttacaata aaacactgaa aatgtccact ttgttagttg tgaacatgag     1902

cccaggccta aggtgctggg aaacagaaag ggcgggagat ttttctttat tctatggcta     1962

gaaaatagtt acctcctctc tgaaagtctt cttcctcatt tctgggtaac agaatatcaa     2022

acaccttgct tataagttat aaagtagtgt tgtccaccat gaacccacca agtaaaaaca     2082

acccaaatac ctatcatgga tgaataatca tgcaagtatc agatctgcac tcaatgccac     2142

acaatgacaa agatagcaaa tgagccacag acggctccac ccaacccaat agatgaacac     2202

ttggttcaaa atcactaaag ctcaaatact cccaggtcaa acaccaggta acaagttaat     2262

actcaacaaa gggggaaaac aaatgttcca ctgaatcctg tgaccctgtg ggcg           2316


<210>  20
<211>  178
<212>  PRT
<213>  Mus musculus

<400>  20

Met Glu Ile Cys Trp Gly Pro Tyr Ser His Leu Ile Ser Leu Leu Leu 
1               5                   10                  15      


Ile Leu Leu Phe His Ser Glu Ala Ala Cys Arg Pro Ser Gly Lys Arg 
            20                  25                  30          


Pro Cys Lys Met Gln Ala Phe Arg Ile Trp Asp Thr Asn Gln Lys Thr 
        35                  40                  45              


Phe Tyr Leu Arg Asn Asn Gln Leu Ile Ala Gly Tyr Leu Gln Gly Pro 
    50                  55                  60                  


Asn Ile Lys Leu Glu Glu Lys Ile Asp Met Val Pro Ile Asp Leu His 
65                  70                  75                  80  


Ser Val Phe Leu Gly Ile His Gly Gly Lys Leu Cys Leu Ser Cys Ala 
                85                  90                  95      


Lys Ser Gly Asp Asp Ile Lys Leu Gln Leu Glu Glu Val Asn Ile Thr 
            100                 105                 110         


Asp Leu Ser Lys Asn Lys Glu Glu Asp Lys Arg Phe Thr Phe Ile Arg 
        115                 120                 125             


Ser Glu Lys Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly 
    130                 135                 140                 


Trp Phe Leu Cys Thr Thr Leu Glu Ala Asp Arg Pro Val Ser Leu Thr 
145                 150                 155                 160 


Asn Thr Pro Glu Glu Pro Leu Ile Val Thr Lys Phe Tyr Phe Gln Glu 
                165                 170                 175     


Asp Gln 
        


<210>  21
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  21

Met Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile 
1               5                   10                  15      


Trp Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val 
            20                  25                  30          


Ala Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp 
        35                  40                  45              


Val Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly 
    50                  55                  60                  


Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln 
65                  70                  75                  80  


Leu Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp 
                85                  90                  95      


Lys Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe 
            100                 105                 110         


Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala 
        115                 120                 125             


Asp Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val 
    130                 135                 140                 


Thr Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150             


