                     

<110>  AB Enzymes Oy
 
<120>  A fungal serine protease and use thereof

<130>  A9360PC

<150>  FI 20095499

<151>  2009-04-30

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of  an aminoterminal peptide #3811 from Fusarium 
       acuminatum RF7182 protease.

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  /replace= "Ile"

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Ala Leu Thr Xaa Gln Ser Asn Ala Pro Trp 
1               5                   10  


<210>  2
<211>  12
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 1609,880 from Fusarium acuminatum 
       RF7182  protease.

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  /replace= "Ile"

<400>  2

Ser Gly Ala Pro Trp Gly Leu Gln Leu Ser His Lys 
1               5                   10          


<210>  3
<211>  18
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 1793,957 from Fusarium acuminatum 
       RF7182  protease.

<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  /replace= "Leu"

<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  /replace= "Leu"

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  /replace= "Ile"

<400>  3

Ala Ala Thr Thr Gln Ser Gly Ala Pro Trp Gly Leu Gly Ala Leu Ser 
1               5                   10                  15      


His Lys 
        


<210>  4
<211>  7
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 743,494 from Fusarium acuminatum 
       RF7182  protease.

<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  /replace= "Asn"

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  /replace= "Asn"

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  /replace= "Ile"

<400>  4

Ala Ala Leu Leu Ser Val Lys 
1               5           


<210>  5
<211>  6
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 2103,832 (start 2045,06) from 
       Fusarium acuminatum RF7182  protease.

<400>  5

Gly Gly Ala His Thr Asp 
1               5       


<210>  6
<211>  14
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 2103,832 (start 1406,57) from 
       Fusarium acuminatum RF7182  protease.

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  /replace= "Ile"

<400>  6

Asn Gly His Gly Thr His Val Ala Gly Thr Leu Asn Thr Lys 
1               5                   10                  


<210>  7
<211>  31
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 3662,692 from Fusarium acuminatum 
       RF7182  protease.

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  /replace= "Ile"

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  /replace= "Ile"

<400>  7

Thr Ser Tyr Leu Tyr Asp Thr Thr Ala Gly Ser Gly Ser Tyr Gly Tyr 
1               5                   10                  15      


Val Val Asp Ser Gly Leu Asn Leu Ala His Tyr Ser Leu Asn Arg 
            20                  25                  30      


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO123 derived from 
       the peptide SEQ ID NO:3.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  w is a or t

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<400>  8
carwcnggng cnccntgggg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO122 derived from 
       the peptide SEQ ID NO:6.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  y is t or c

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  y is t or c

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<400>  9
cayggnacnc aygtngcngg                                                   20


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the serine protease consensus oligonucleotide 
       primer PRO60.


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  m is a or c

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  w is a or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  s is c or g

<400>  10
gmrgccatrg aggtwccrgt sa                                                22


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the serine protease consensus oligonucleotide 
       primer PRO61.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  m is a or c

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  m is a or c

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  w is a or t

<400>  11
ggmgmrgcca trgaggtwcc                                                   20


<210>  12
<211>  753
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the PCR fragment obtained using the primers 
       PRO123 (SEQ ID NO:8) and PRO61 (SEQ ID NO:11) and Fusarium 
       acuminatum RF7182 genomic DNA as a template.

<400>  12
cagtctggtg cgccctgggg tctcggtgcc atctcccaca agtcgtctgg ctccaccagc       60

tacatctacg acaccactgc cggtagcggc tcttacggat atgtcgtaga tagtggtatc      120

aacatcgccc ataccgactt tggtggccgt gctactctcg gctacaacgc tgctggtggt      180

gctcacaccg ataccctcgg ccacggaacc cacgttgctg gtaccatcgg tggcaccaag      240

tatggtgtct ccaagaaggc caacctcatc tctgtcaagg tcttcgccgg taaccaggct      300

gctacatctg ttatccttga tggctttaac tgggccgtca aacgacatca cctccaaggg      360

ccgtgctggc aagtccgtta tcaacatgtc tctcggtaag tagtttcgca ctcagaccta      420

tacatggaaa tcactaacca agacaccagg cggaccttct tctgctactt ggaccactgc      480

catcaacgct ggatacaacg ctggtgtcct ctccgttgtc gctgccggta acggtgatgt      540

caatggcaac cctctccccg tctctagcca gtctcctgcc aacgccccca acgccctgac      600

cgtcgctgcc attgactcca actggcgcac tgcctctttc accaactacg gtgccggtgt      660

tgatatcttc ggccccggtg tcaacattct gtccgcctgg atcggctcca gcaccgctac      720

caacaccatc agcggaacct ccatggcctc gcc                                   753


<210>  13
<211>  1353
<212>  DNA
<213>  Fusarium acuminatum


<220>
<221>  misc_feature
<223>  The nucleotide sequence of the full-length Fusarium acuminatum 
       RF7182 protease gene (Fa prtS8A).

<400>  13
atgactagca tccgccgcct cgctctctac cttggagctc tgctccctgc agtgcttgct       60

gctcctgctg gcgtctccaa ggtcgctaag ctcgactctg tgcccgacaa gtacatcatc      120

accctcaagc ctgattcctc tgacgccaag gtcgaggctc acttgagctg ggtcgagggc      180

gtccaccgcc gcagccttgc taagcgcgac accgtcggtg ttgagaagac tttcaacatc      240

agcagctgga gcgcttactc tggcgagttc gaccaggcta ccattgatga gatcaagaag      300

agccctgagg taagccttcc ggtactggcc taagtaacac aaatcaccaa catgccatag      360

gttgctttcg ttgagcctga ctacactgtc tacctggact atgaggagtc atctgagctg      420

tctgaccgcg ctctgaccac ccagtctggt gctccctggg gtctcggtgc catctcccac      480

aagtcgtctg gctccaccag ctacatctac gacaccactg ccggtagcgg ctcttacgga      540

tatgtcgtag atagtggtat caacatcgcc cataccgact ttggtggccg tgctactctc      600

ggctacaacg ctgctggtgg tgctcacacc gataccctcg gccacggaac ccacgttgct      660

ggtaccatcg gtggcaccaa gtatggtgtc tccaagaagg ccaacctcat ctctgtcaag      720

gtcttcgccg gtaaccaggc tgctacatct gttatccttg atggctttaa ctgggccgtc      780

aacgacatca cctccaaggg ccgtgctggc aagtccgtta tcaacatgtc tctcggtaag      840

tagtttcgca ctcagaccta tacatggaaa tcactaacca agacaccagg cggaccttct      900

tctgctactt ggaccactgc catcaacgct ggatacaacg ctggtgtcct ctccgttgtc      960

gctgccggta acggtgatgt caatggcaac cctctccccg tctctagcca gtctcctgcc     1020

aacgccccca acgccctgac cgtcgctgcc attgactcca actggcgcac tgcctctttc     1080

accaactacg gtgccggtgt tgatatcttc ggccccggtg tcaacattct gtccgcctgg     1140

atcggctcca gcaccgctac caacaccatc agcggaactt ccatggcctc cccccacctt     1200

gctggtcttg ctctctacct ccaggtcctt gagggtctta gcactcctgc tgctgtcacc     1260

aaccgcatca aggctcttgg tacctctggc aaggtcactg gtagcctcag cggcagcccc     1320

aacctcgttg cctacaacgg taacggtgct tag                                  1353


<210>  14
<211>  415
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  misc_feature
<223>  The deduced amino acid sequence of the full-length Fusarium 
       acuminatum RF7182 protease (Fa_RF7182), including amino acids 
       Met1 to Ala415.

<400>  14

Met Thr Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro 
1               5                   10                  15      


Ala Val Leu Ala Ala Pro Ala Gly Val Ser Lys Val Ala Lys Leu Asp 
            20                  25                  30          


Ser Val Pro Asp Lys Tyr Ile Ile Thr Leu Lys Pro Asp Ser Ser Asp 
        35                  40                  45              


Ala Lys Val Glu Ala His Leu Ser Trp Val Glu Gly Val His Arg Arg 
    50                  55                  60                  


Ser Leu Ala Lys Arg Asp Thr Val Gly Val Glu Lys Thr Phe Asn Ile 
65                  70                  75                  80  


Ser Ser Trp Ser Ala Tyr Ser Gly Glu Phe Asp Gln Ala Thr Ile Asp 
                85                  90                  95      


Glu Ile Lys Lys Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Thr 
            100                 105                 110         


Val Tyr Leu Asp Tyr Glu Glu Ser Ser Glu Leu Ser Asp Arg Ala Leu 
        115                 120                 125             


Thr Thr Gln Ser Gly Ala Pro Trp Gly Leu Gly Ala Ile Ser His Lys 
    130                 135                 140                 


Ser Ser Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Thr Ala Gly Ser Gly 
145                 150                 155                 160 


Ser Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Ile Ala His Thr Asp 
                165                 170                 175     


Phe Gly Gly Arg Ala Thr Leu Gly Tyr Asn Ala Ala Gly Gly Ala His 
            180                 185                 190         


Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile Gly Gly 
        195                 200                 205             


Thr Lys Tyr Gly Val Ser Lys Lys Ala Asn Leu Ile Ser Val Lys Val 
    210                 215                 220                 


Phe Ala Gly Asn Gln Ala Ala Thr Ser Val Ile Leu Asp Gly Phe Asn 
225                 230                 235                 240 


Trp Ala Val Asn Asp Ile Thr Ser Lys Gly Arg Ala Gly Lys Ser Val 
                245                 250                 255     


Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Ala Thr Trp Thr Thr Ala 
            260                 265                 270         


Ile Asn Ala Gly Tyr Asn Ala Gly Val Leu Ser Val Val Ala Ala Gly 
        275                 280                 285             


Asn Gly Asp Val Asn Gly Asn Pro Leu Pro Val Ser Ser Gln Ser Pro 
    290                 295                 300                 


Ala Asn Ala Pro Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Asn Trp 
305                 310                 315                 320 


Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Ile Phe Gly 
                325                 330                 335     


Pro Gly Val Asn Ile Leu Ser Ala Trp Ile Gly Ser Ser Thr Ala Thr 
            340                 345                 350         


Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Leu Ala Gly Leu 
        355                 360                 365             


Ala Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ala Val 
    370                 375                 380                 


Thr Asn Arg Ile Lys Ala Leu Gly Thr Ser Gly Lys Val Thr Gly Ser 
385                 390                 395                 400 


Leu Ser Gly Ser Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly Ala 
                405                 410                 415 


<210>  15
<211>  1293
<212>  DNA
<213>  Fusarium acuminatum


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       proenzyme form of Fusarium acuminatum RF7182 protease.

<400>  15
gctcctgctg gcgtctccaa ggtcgctaag ctcgactctg tgcccgacaa gtacatcatc       60

accctcaagc ctgattcctc tgacgccaag gtcgaggctc acttgagctg ggtcgagggc      120

gtccaccgcc gcagccttgc taagcgcgac accgtcggtg ttgagaagac tttcaacatc      180

agcagctgga gcgcttactc tggcgagttc gaccaggcta ccattgatga gatcaagaag      240

agccctgagg taagccttcc ggtactggcc taagtaacac aaatcaccaa catgccatag      300

gttgctttcg ttgagcctga ctacactgtc tacctggact atgaggagtc atctgagctg      360

tctgaccgcg ctctgaccac ccagtctggt gctccctggg gtctcggtgc catctcccac      420

aagtcgtctg gctccaccag ctacatctac gacaccactg ccggtagcgg ctcttacgga      480

tatgtcgtag atagtggtat caacatcgcc cataccgact ttggtggccg tgctactctc      540

ggctacaacg ctgctggtgg tgctcacacc gataccctcg gccacggaac ccacgttgct      600

ggtaccatcg gtggcaccaa gtatggtgtc tccaagaagg ccaacctcat ctctgtcaag      660

gtcttcgccg gtaaccaggc tgctacatct gttatccttg atggctttaa ctgggccgtc      720

aacgacatca cctccaaggg ccgtgctggc aagtccgtta tcaacatgtc tctcggtaag      780

tagtttcgca ctcagaccta tacatggaaa tcactaacca agacaccagg cggaccttct      840

tctgctactt ggaccactgc catcaacgct ggatacaacg ctggtgtcct ctccgttgtc      900

gctgccggta acggtgatgt caatggcaac cctctccccg tctctagcca gtctcctgcc      960

aacgccccca acgccctgac cgtcgctgcc attgactcca actggcgcac tgcctctttc     1020

accaactacg gtgccggtgt tgatatcttc ggccccggtg tcaacattct gtccgcctgg     1080

atcggctcca gcaccgctac caacaccatc agcggaactt ccatggcctc cccccacctt     1140

gctggtcttg ctctctacct ccaggtcctt gagggtctta gcactcctgc tgctgtcacc     1200

aaccgcatca aggctcttgg tacctctggc aaggtcactg gtagcctcag cggcagcccc     1260

aacctcgttg cctacaacgg taacggtgct tag                                  1293


<210>  16
<211>  395
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the  proenzyme form of Fusarium 
       acuminatum RF7182 protease, including amino acids Ala21 to Ala415
       of the full-length protease.

<400>  16

Ala Pro Ala Gly Val Ser Lys Val Ala Lys Leu Asp Ser Val Pro Asp 
1               5                   10                  15      


Lys Tyr Ile Ile Thr Leu Lys Pro Asp Ser Ser Asp Ala Lys Val Glu 
            20                  25                  30          


Ala His Leu Ser Trp Val Glu Gly Val His Arg Arg Ser Leu Ala Lys 
        35                  40                  45              


Arg Asp Thr Val Gly Val Glu Lys Thr Phe Asn Ile Ser Ser Trp Ser 
    50                  55                  60                  


Ala Tyr Ser Gly Glu Phe Asp Gln Ala Thr Ile Asp Glu Ile Lys Lys 
65                  70                  75                  80  


Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Thr Val Tyr Leu Asp 
                85                  90                  95      


Tyr Glu Glu Ser Ser Glu Leu Ser Asp Arg Ala Leu Thr Thr Gln Ser 
            100                 105                 110         


Gly Ala Pro Trp Gly Leu Gly Ala Ile Ser His Lys Ser Ser Gly Ser 
        115                 120                 125             


Thr Ser Tyr Ile Tyr Asp Thr Thr Ala Gly Ser Gly Ser Tyr Gly Tyr 
    130                 135                 140                 


Val Val Asp Ser Gly Ile Asn Ile Ala His Thr Asp Phe Gly Gly Arg 
145                 150                 155                 160 


Ala Thr Leu Gly Tyr Asn Ala Ala Gly Gly Ala His Thr Asp Thr Leu 
                165                 170                 175     


Gly His Gly Thr His Val Ala Gly Thr Ile Gly Gly Thr Lys Tyr Gly 
            180                 185                 190         


Val Ser Lys Lys Ala Asn Leu Ile Ser Val Lys Val Phe Ala Gly Asn 
        195                 200                 205             


Gln Ala Ala Thr Ser Val Ile Leu Asp Gly Phe Asn Trp Ala Val Asn 
    210                 215                 220                 


Asp Ile Thr Ser Lys Gly Arg Ala Gly Lys Ser Val Ile Asn Met Ser 
225                 230                 235                 240 


Leu Gly Gly Pro Ser Ser Ala Thr Trp Thr Thr Ala Ile Asn Ala Gly 
                245                 250                 255     


Tyr Asn Ala Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Val 
            260                 265                 270         


Asn Gly Asn Pro Leu Pro Val Ser Ser Gln Ser Pro Ala Asn Ala Pro 
        275                 280                 285             


Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Asn Trp Arg Thr Ala Ser 
    290                 295                 300                 


Phe Thr Asn Tyr Gly Ala Gly Val Asp Ile Phe Gly Pro Gly Val Asn 
305                 310                 315                 320 


Ile Leu Ser Ala Trp Ile Gly Ser Ser Thr Ala Thr Asn Thr Ile Ser 
                325                 330                 335     


Gly Thr Ser Met Ala Ser Pro His Leu Ala Gly Leu Ala Leu Tyr Leu 
            340                 345                 350         


Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ala Val Thr Asn Arg Ile 
        355                 360                 365             


Lys Ala Leu Gly Thr Ser Gly Lys Val Thr Gly Ser Leu Ser Gly Ser 
    370                 375                 380                 


Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly Ala 
385                 390                 395 


<210>  17
<211>  924
<212>  DNA
<213>  Fusarium acuminatum


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fusarium acuminatum RF7182 protease.

<400>  17
gctctgacca cccagtctgg tgctccctgg ggtctcggtg ccatctccca caagtcgtct       60

ggctccacca gctacatcta cgacaccact gccggtagcg gctcttacgg atatgtcgta      120

gatagtggta tcaacatcgc ccataccgac tttggtggcc gtgctactct cggctacaac      180

gctgctggtg gtgctcacac cgataccctc ggccacggaa cccacgttgc tggtaccatc      240

ggtggcacca agtatggtgt ctccaagaag gccaacctca tctctgtcaa ggtcttcgcc      300

ggtaaccagg ctgctacatc tgttatcctt gatggcttta actgggccgt caacgacatc      360

acctccaagg gccgtgctgg caagtccgtt atcaacatgt ctctcggtaa gtagtttcgc      420

actcagacct atacatggaa atcactaacc aagacaccag gcggaccttc ttctgctact      480

tggaccactg ccatcaacgc tggatacaac gctggtgtcc tctccgttgt cgctgccggt      540

aacggtgatg tcaatggcaa ccctctcccc gtctctagcc agtctcctgc caacgccccc      600

aacgccctga ccgtcgctgc cattgactcc aactggcgca ctgcctcttt caccaactac      660

ggtgccggtg ttgatatctt cggccccggt gtcaacattc tgtccgcctg gatcggctcc      720

agcaccgcta ccaacaccat cagcggaact tccatggcct ccccccacct tgctggtctt      780

gctctctacc tccaggtcct tgagggtctt agcactcctg ctgctgtcac caaccgcatc      840

aaggctcttg gtacctctgg caaggtcact ggtagcctca gcggcagccc caacctcgtt      900

gcctacaacg gtaacggtgc ttag                                             924


<210>  18
<211>  289
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Fusarium acuminatum
       RF7182 protease, including amino acids Ala127 to Ala415 of the 
       full-length protease.

<400>  18

Ala Leu Thr Thr Gln Ser Gly Ala Pro Trp Gly Leu Gly Ala Ile Ser 
1               5                   10                  15      


His Lys Ser Ser Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ser Gly Ser Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Ile Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Thr Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Gly Gly Thr Lys Tyr Gly Val Ser Lys Lys Ala Asn Leu Ile Ser Val 
                85                  90                  95      


Lys Val Phe Ala Gly Asn Gln Ala Ala Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Gly Arg Ala Gly Lys 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Ala Thr Trp Thr 
    130                 135                 140                 


Thr Ala Ile Asn Ala Gly Tyr Asn Ala Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Val Asn Gly Asn Pro Leu Pro Val Ser Ser Gln 
                165                 170                 175     


Ser Pro Ala Asn Ala Pro Asn Ala Leu Thr Val Ala Ala Ile Asp Ser 
            180                 185                 190         


Asn Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Leu Ser Ala Trp Ile Gly Ser Ser Thr 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Leu Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ala Leu Gly Thr Ser Gly Lys Val Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly 
        275                 280                 285             


Ala 
    


