

<110>  AB Enzymes Oy
 
<120>  A novel fungal protease and use thereof

<130>  A9273PC

<150>  FI 20095497

<151>  2009-04-30

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  Sequence of  an aminoterminal peptide #3792 from Fusarium 
       equiseti RF6318 protease.

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  /replace= "Ser"

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  /replace= "Thr"

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  /replace= "Arg"

<400>  1

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Cys Thr Pro 
            20  


<210>  2
<211>  10
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 1246.673 from Fusarium equiseti 
       RF6318  protease.

<400>  2

Thr Val Ala Ala Ala Asp Ser Ser Trp Arg 
1               5                   10  


<210>  3
<211>  7
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 3341.633 from Fusarium equiseti 
       RF6318  protease.

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Xaa Thr Tyr Gly Val Ala Lys 
1               5           


<210>  4
<211>  14
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  Sequence of a tryptic peptide 1503.799 from Fusarium equiseti 
       RF6318 protease

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  /replace= "Ile"

<400>  4

Glu Ala Leu Thr Val Gly Ala Thr Thr Ser Ala Asp Ala Lys 
1               5                   10                  


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO87 derived from the
       aminoterminal peptide SEQ ID NO:1.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  y is t or c

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<400>  5
cartcnaayg cnccntgggg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO88 derived from the
       aminoterminal peptide SEQ ID NO:1.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  y is t or c

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  y is t or c

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<400>  6
caragyaayg cnccntgggg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO89 derived from 
       peptide SEQ ID NO:4.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<400>  7
gcrtcngcng angtngtngc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the oligonucleotide primer PRO90 derived from 
       peptide SEQ ID NO:4.


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  r is a or g

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<400>  8
gcrtcngcrc tngtngtngc                                                   20


<210>  9
<211>  866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The sequence of the PCR fragment obtained using the primers PRO88
       (SEQ ID NO:6) and PRO89 (SEQ ID NO:7) and Fusarium equiseti 
       RF6318 genomic DNA as a template.

<400>  9
aaagcaacgc accgtggggt cttgctgcca tctcccgccg aacccccggt ggcagcacct       60

acacctacga caccactgcc ggtgccggta cttacggtta cgtcgttgac tctggtatca      120

acaccgccca cactgacttt ggcggccgtg cttctctcgg ttacaacgct gctggtggcg      180

cccacactga tacccttggc cacggtaccc acgttgctgg taccattgcc tccaacacct      240

acggtgttgc caagcgtgta agtacaatca taccccacat gagctacaac atgatctgaa      300

ctttatactt actattatta ggccaacgtc atctctgtca aggttttcgt cggtaaccaa      360

gcttctacct ctgttatcct tgctggtttc aactgggctg tcaacgacat cacctccaga      420

accgtgctag cccgctctgt catcaacatg tctctcggtg gtccctcttc tcagacctgg      480

gctactgcca tcaacgctgc ctacagccaa ggtgtcctct ccgttgttgc tgccggtaac      540

ggtgattcca acggtcgtcc tctccccgcc tctggccagt ctcctgccaa cgttcccaac      600

gctatcaccg ttgctgccgc cgactccagc tggcgaactg cctctttcac caactacggt      660

cctgaggtcg atgtcttcgg tcctggtgtc aacatccagt ccacctggta cacctccaac      720

agcgctacca acaccatcag cggtacctcc atggcttgcc ctcacgttgc tggtcttgct      780

ctctacctcc aggctctcga gaacctcaat acccctgctg ccgtcaccaa ccgcatcaag      840

tctcttgcaa ctacctccgc tgacgc                                           866


<210>  10
<211>  1303
<212>  DNA
<213>  Fusarium equiseti


<220>
<221>  misc_feature
<223>  The nucleotide sequence of the full-length Fusarium equiseti 
       RF6318  protease gene (Fe prtS8A) .

<400>  10
atgactagct tccgccgtat cgctcttggc cttgcagctc tgctgcccgc agtcctcgcc       60

gctcccaccg agaagcgaca ggagctcact gccgcgcctg acaagtacat catcaccctc      120

aagcccgagg ctgctgaggc caaggtcgag gctcacatgg cctgggttac cgacgtccac      180

cgccgcagcc tcggcaagcg tgacacttcc ggtgttgaga agaagttcaa catcagcagc      240

tggaacgcct actctggcga gttcgacgat gctaccattg ctgagatcaa gaagagcccc      300

gaggttgcct tcgtcgagcc cgactacatt gtcaccctcg actacaaggt tgagcctctc      360

tctgaccgtg ctctgaccac tcagagcaac gctccttggg gtcttgctgc catctcccgc      420

cgaacccccg gtggcagcac ctacacctac gacaccactg ccggtgccgg tacttacggt      480

tacgtcgttg actctggtat caacaccgcc cacactgact ttggcggccg tgcttctctc      540

ggttacaacg ctgctggtgg cgcccacact gatacccttg gccacggtac ccacgttgct      600

ggtaccattg cctccaacac ctacggtgtt gccaagcgtg taagtacaat cataccccac      660

atgagctaca acatgatctg aactttatac ttactattat taggccaacg tcatctctgt      720

caaggttttc gtcggtaacc aagcttctac ctctgttatc cttgctggtt tcaactgggc      780

tgtcaacgac atcacctcca agaaccgtgc tagccgctct gtcatcaaca tgtctctcgg      840

tggtccctct tctcagacct gggctactgc catcaacgct gcctacagcc aaggtgtcct      900

ctccgttgtt gctgccggta acggtgattc caacggtcgt cctctccccg cctctggcca      960

gtctcctgcc aacgttccca acgctatcac cgttgctgcc gccgactcca gctggcgaac     1020

tgcctctttc accaactacg gtcctgaggt cgatgtcttc ggtcctggtg tcaacatcca     1080

gtccacctgg tacacctcca acagcgctac caacaccatc agcggtacct ccatggcttg     1140

ccctcacgtt gctggtcttg ctctctacct ccaggctctc gagaacctca atacccctgc     1200

tgccgtcacc aaccgcatca agtctcttgc cactaccggc cgcatcactg gcagcctcag     1260

cggcagcccc aacgccatgg ctttcaacgg cgctactgct taa                       1303


<210>  11
<211>  412
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  The deduced amino acid sequence of the full-length Fusarium 
       equiseti RF6318 protease (Fe_RF6318) including amino acids from 
       Met1 to Ala412.

<400>  11

Met Thr Ser Phe Arg Arg Ile Ala Leu Gly Leu Ala Ala Leu Leu Pro 
1               5                   10                  15      


Ala Val Leu Ala Ala Pro Thr Glu Lys Arg Gln Glu Leu Thr Ala Ala 
            20                  25                  30          


Pro Asp Lys Tyr Ile Ile Thr Leu Lys Pro Glu Ala Ala Glu Ala Lys 
        35                  40                  45              


Val Glu Ala His Met Ala Trp Val Thr Asp Val His Arg Arg Ser Leu 
    50                  55                  60                  


Gly Lys Arg Asp Thr Ser Gly Val Glu Lys Lys Phe Asn Ile Ser Ser 
65                  70                  75                  80  


Trp Asn Ala Tyr Ser Gly Glu Phe Asp Asp Ala Thr Ile Ala Glu Ile 
                85                  90                  95      


Lys Lys Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Ile Val Thr 
            100                 105                 110         


Leu Asp Tyr Lys Val Glu Pro Leu Ser Asp Arg Ala Leu Thr Thr Gln 
        115                 120                 125             


Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser Arg Arg Thr Pro Gly 
    130                 135                 140                 


Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly Ala Gly Thr Tyr Gly 
145                 150                 155                 160 


Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His Thr Asp Phe Gly Gly 
                165                 170                 175     


Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly Ala His Thr Asp Thr 
            180                 185                 190         


Leu Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ser Asn Thr Tyr 
        195                 200                 205             


Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val Lys Val Phe Val Gly 
    210                 215                 220                 


Asn Gln Ala Ser Thr Ser Val Ile Leu Ala Gly Phe Asn Trp Ala Val 
225                 230                 235                 240 


Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg Ser Val Ile Asn Met 
                245                 250                 255     


Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala Thr Ala Ile Asn Ala 
            260                 265                 270         


Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp 
        275                 280                 285             


Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln Ser Pro Ala Asn Val 
    290                 295                 300                 


Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser Ser Trp Arg Thr Ala 
305                 310                 315                 320 


Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Val Phe Gly Pro Gly Val 
                325                 330                 335     


Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser Ala Thr Asn Thr Ile 
            340                 345                 350         


Ser Gly Thr Ser Met Ala Cys Pro His Val Ala Gly Leu Ala Leu Tyr 
        355                 360                 365             


Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala Ala Val Thr Asn Arg 
    370                 375                 380                 


Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr Gly Ser Leu Ser Gly 
385                 390                 395                 400 


Ser Pro Asn Ala Met Ala Phe Asn Gly Ala Thr Ala 
                405                 410         


<210>  12
<211>  1243
<212>  DNA
<213>  Fusarium equiseti


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       proenzyme form of Fusarium equiseti RF6318  protease.

<400>  12
gctcccaccg agaagcgaca ggagctcact gccgcgcctg acaagtacat catcaccctc       60

aagcccgagg ctgctgaggc caaggtcgag gctcacatgg cctgggttac cgacgtccac      120

cgccgcagcc tcggcaagcg tgacacttcc ggtgttgaga agaagttcaa catcagcagc      180

tggaacgcct actctggcga gttcgacgat gctaccattg ctgagatcaa gaagagcccc      240

gaggttgcct tcgtcgagcc cgactacatt gtcaccctcg actacaaggt tgagcctctc      300

tctgaccgtg ctctgaccac tcagagcaac gctccttggg gtcttgctgc catctcccgc      360

cgaacccccg gtggcagcac ctacacctac gacaccactg ccggtgccgg tacttacggt      420

tacgtcgttg actctggtat caacaccgcc cacactgact ttggcggccg tgcttctctc      480

ggttacaacg ctgctggtgg cgcccacact gatacccttg gccacggtac ccacgttgct      540

ggtaccattg cctccaacac ctacggtgtt gccaagcgtg taagtacaat cataccccac      600

atgagctaca acatgatctg aactttatac ttactattat taggccaacg tcatctctgt      660

caaggttttc gtcggtaacc aagcttctac ctctgttatc cttgctggtt tcaactgggc      720

tgtcaacgac atcacctcca agaaccgtgc tagccgctct gtcatcaaca tgtctctcgg      780

tggtccctct tctcagacct gggctactgc catcaacgct gcctacagcc aaggtgtcct      840

ctccgttgtt gctgccggta acggtgattc caacggtcgt cctctccccg cctctggcca      900

gtctcctgcc aacgttccca acgctatcac cgttgctgcc gccgactcca gctggcgaac      960

tgcctctttc accaactacg gtcctgaggt cgatgtcttc ggtcctggtg tcaacatcca     1020

gtccacctgg tacacctcca acagcgctac caacaccatc agcggtacct ccatggcttg     1080

ccctcacgtt gctggtcttg ctctctacct ccaggctctc gagaacctca atacccctgc     1140

tgccgtcacc aaccgcatca agtctcttgc cactaccggc cgcatcactg gcagcctcag     1200

cggcagcccc aacgccatgg ctttcaacgg cgctactgct taa                       1243


<210>  13
<211>  392
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the proenzyme form of Fusarium 
       equiseti RF6318 protease including amino acids Ala21 to Ala 412 
       of the full length protease.

<400>  13

Ala Pro Thr Glu Lys Arg Gln Glu Leu Thr Ala Ala Pro Asp Lys Tyr 
1               5                   10                  15      


Ile Ile Thr Leu Lys Pro Glu Ala Ala Glu Ala Lys Val Glu Ala His 
            20                  25                  30          


Met Ala Trp Val Thr Asp Val His Arg Arg Ser Leu Gly Lys Arg Asp 
        35                  40                  45              


Thr Ser Gly Val Glu Lys Lys Phe Asn Ile Ser Ser Trp Asn Ala Tyr 
    50                  55                  60                  


Ser Gly Glu Phe Asp Asp Ala Thr Ile Ala Glu Ile Lys Lys Ser Pro 
65                  70                  75                  80  


Glu Val Ala Phe Val Glu Pro Asp Tyr Ile Val Thr Leu Asp Tyr Lys 
                85                  90                  95      


Val Glu Pro Leu Ser Asp Arg Ala Leu Thr Thr Gln Ser Asn Ala Pro 
            100                 105                 110         


Trp Gly Leu Ala Ala Ile Ser Arg Arg Thr Pro Gly Gly Ser Thr Tyr 
        115                 120                 125             


Thr Tyr Asp Thr Thr Ala Gly Ala Gly Thr Tyr Gly Tyr Val Val Asp 
    130                 135                 140                 


Ser Gly Ile Asn Thr Ala His Thr Asp Phe Gly Gly Arg Ala Ser Leu 
145                 150                 155                 160 


Gly Tyr Asn Ala Ala Gly Gly Ala His Thr Asp Thr Leu Gly His Gly 
                165                 170                 175     


Thr His Val Ala Gly Thr Ile Ala Ser Asn Thr Tyr Gly Val Ala Lys 
            180                 185                 190         


Arg Ala Asn Val Ile Ser Val Lys Val Phe Val Gly Asn Gln Ala Ser 
        195                 200                 205             


Thr Ser Val Ile Leu Ala Gly Phe Asn Trp Ala Val Asn Asp Ile Thr 
    210                 215                 220                 


Ser Lys Asn Arg Ala Ser Arg Ser Val Ile Asn Met Ser Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Ser Gln Thr Trp Ala Thr Ala Ile Asn Ala Ala Tyr Ser Gln 
                245                 250                 255     


Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Ser Asn Gly Arg 
            260                 265                 270         


Pro Leu Pro Ala Ser Gly Gln Ser Pro Ala Asn Val Pro Asn Ala Ile 
        275                 280                 285             


Thr Val Ala Ala Ala Asp Ser Ser Trp Arg Thr Ala Ser Phe Thr Asn 
    290                 295                 300                 


Tyr Gly Pro Glu Val Asp Val Phe Gly Pro Gly Val Asn Ile Gln Ser 
305                 310                 315                 320 


Thr Trp Tyr Thr Ser Asn Ser Ala Thr Asn Thr Ile Ser Gly Thr Ser 
                325                 330                 335     


Met Ala Cys Pro His Val Ala Gly Leu Ala Leu Tyr Leu Gln Ala Leu 
            340                 345                 350         


Glu Asn Leu Asn Thr Pro Ala Ala Val Thr Asn Arg Ile Lys Ser Leu 
        355                 360                 365             


Ala Thr Thr Gly Arg Ile Thr Gly Ser Leu Ser Gly Ser Pro Asn Ala 
    370                 375                 380                 


Met Ala Phe Asn Gly Ala Thr Ala 
385                 390         


<210>  14
<211>  934
<212>  DNA
<213>  Fusarium equiseti


<220>
<221>  misc_feature
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fusarium equiseti RF6318 protease.

<400>  14
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gtaagtacaa tcatacccca catgagctac      300

aacatgatct gaactttata cttactatta ttaggccaac gtcatctctg tcaaggtttt      360

cgtcggtaac caagcttcta cctctgttat ccttgctggt ttcaactggg ctgtcaacga      420

catcacctcc aagaaccgtg ctagccgctc tgtcatcaac atgtctctcg gtggtccctc      480

ttctcagacc tgggctactg ccatcaacgc tgcctacagc caaggtgtcc tctccgttgt      540

tgctgccggt aacggtgatt ccaacggtcg tcctctcccc gcctctggcc agtctcctgc      600

caacgttccc aacgctatca ccgttgctgc cgccgactcc agctggcgaa ctgcctcttt      660

caccaactac ggtcctgagg tcgatgtctt cggtcctggt gtcaacatcc agtccacctg      720

gtacacctcc aacagcgcta ccaacaccat cagcggtacc tccatggctt gccctcacgt      780

tgctggtctt gctctctacc tccaggctct cgagaacctc aatacccctg ctgccgtcac      840

caaccgcatc aagtctcttg ccactaccgg ccgcatcact ggcagcctca gcggcagccc      900

caacgccatg gctttcaacg gcgctactgc ttaa                                  934


<210>  15
<211>  289
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Fusarium equiseti 
       RF6318 protease including amino acids Ala124 to Ala412 of the 
       full legth enzyme.

<400>  15

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Ala Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Val 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Ala Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


