                         SEQUENCE LISTING

<110>  Codexis, Inc.
       Pollack, Jeffrey S.
       Alvizo, Oscar
       Galgoczy, David J.
       Saran, Dayal
 
<120>  InhA Promoters

<130>  CX3-013US1

<150>  US 61/169,848
<151>  2009-04-16

<160>  63    

<170>  PatentIn version 3.3

<210>  1
<211>  536
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence from promoter of inhA gene from B. 
       megaterium

<400>  1
gagctggaaa agaaagggaa atccgtgagt tatgaagggg atttcttgtt taaaattgat     60

gaaaaacctt atgaaatcgc agcgcgcaat atgtctattt ggagcatgtc gctctatcat    120

atcttgctgc agcgtcaaaa agaacattct taaaatttat ttgtatacag agattatcgc    180

ttctcgtcta ttgagaagtg atttttttat ctttctgtat ccataatggg gaattttttg    240

tttttatata atagtaatac ggaaaagata gttctaattc cctaaaaaaa tacacttcat    300

atatttttag ttttggttat cgatttggca tggaatttgc atgtgtaaga agggtttatc    360

agacatttta attgtcagga aaagaaaact agttacatac tataaaacta ttttacatta    420

tctactagaa tcaaaaaatt ttaataatat agtaaaatgt agttgacctt cctagtttta    480

gaatataaaa taaatcttgc taatagtttg aaaattctaa aatggagggg tttact        536


<210>  2
<211>  541
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence from inhA promoter

<400>  2
gagctggaaa agaaagggaa atccgtgagt tatgaagggg atttcttgtt taaaattgat     60

gaaaaacctt atgaaatcgc agcgcgcaat atgtctattt ggagcatgtc gctctatcat    120

atcttgctgc agcgtcaaaa agaacattct taaaatttat ttgtatacag agattatcgc    180

ttctcgtcta ttgagaagtg atttttttat ctttctgtat ccataatggg gaattttttg    240

tttttatata atagtaatac ggaaaagata gttctaattc cctaaaaaaa tacacttcat    300

atatttttag ttttggttat cgatttggca tggaatttgc atgtgtaaga agggtttatc    360

agacatttta attgtcagga aaagaaaact agttacatac tataaaacta ttttacatta    420

tctactagaa tcaaaaaatt ttaataatat agtaaaatgt agttgacctt cctagtttta    480

gaatataaaa taaatcttgc taatagtttg aaaattctaa aatggagggg tttactgtac    540

a                                                                    541


<210>  3
<211>  522
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence from inhA promoter

<400>  3
gagctggaaa agaaagggaa atccgtgagt tatgaagggg atttcttgtt taaaattgat     60

gaaaaacctt atgaaatcgc agcgcgcaat atgtctattt ggagcatgtc gctctatcat    120

atcttgctgc agcgtcaaaa agaacattct taaaatttat ttgtatacag agattatcgc    180

ttctcgtcta ttgagaagtg atttttttat ctttctgtat ccataatggg gaattttttg    240

tttttatata atagtaatac ggaaaagata gttctaattc cctaaaaaaa tacacttcat    300

atatttttag ttttggttat cgatttggca tggaatttgc atgtgtaaga agggtttatc    360

agacatttta attgtcagga aaagaaaact agttacatac tataaaacta ttttacatta    420

tctactagaa tcaaaaaatt ttaataatat agtaaaatgt agttgacctt cctagtttta    480

gaatataaaa taaatcttgc taatagtttg aaaattctaa aa                       522


<210>  4
<211>  349
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated region from promoter of inhA 
       gene from B. megaterium

<400>  4
ttatcgcttc tcgtctattg agaagtgatt tttttatctt tctgtatcca taatggggaa     60

ttttttgttt ttatataata gtaatacgga aaagatagtt ctaattccct aaaaaaatac    120

acttcatata tttttagttt tggttatcga tttggcatgg aatttgcatg tgtaagaagg    180

gtttatcaga cattttaatt gtcaggaaaa gaaaactagt tacatactat aaaactattt    240

tacattatct actagaatca aaaaatttta ataatatagt aaaatgtagt tgaccttcct    300

agttttagaa tataaaataa atcttgctaa tagtttgaaa attctaaaa                349


<210>  5
<211>  249
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated region from promoter of inhA 
       gene from B. megaterium

<400>  5
ctaattccct aaaaaaatac acttcatata tttttagttt tggttatcga tttggcatgg     60

aatttgcatg tgtaagaagg gtttatcaga cattttaatt gtcaggaaaa gaaaactagt    120

tacatactat aaaactattt tacattatct actagaatca aaaaatttta ataatatagt    180

aaaatgtagt tgaccttcct agttttagaa tataaaataa atcttgctaa tagtttgaaa    240

attctaaaa                                                            249


<210>  6
<211>  199
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated region from promoter of inhA 
       gene from B. megaterium

<400>  6
tttggcatgg aatttgcatg tgtaagaagg gtttatcaga cattttaatt gtcaggaaaa     60

gaaaactagt tacatactat aaaactattt tacattatct actagaatca aaaaatttta    120

ataatatagt aaaatgtagt tgaccttcct agttttagaa tataaaataa atcttgctaa    180

tagtttgaaa attctaaaa                                                 199


<210>  7
<211>  124
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated region from promoter of inhA 
       gene from B. megaterium

<400>  7
actataaaac tattttacat tatctactag aatcaaaaaa ttttaataat atagtaaaat     60

gtagttgacc ttcctagttt tagaatataa aataaatctt gctaatagtt tgaaaattct    120

aaaa                                                                 124


<210>  8
<211>  2624
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of inhA/celA construct

<400>  8
gagctggaaa agaaagggaa atccgtgagt tatgaagggg atttcttgtt taaaattgat     60

gaaaaacctt atgaaatcgc agcgcgcaat atgtctattt ggagcatgtc gctctatcat    120

atcttgctgc agcgtcaaaa agaacattct taaaatttat ttgtatacag agattatcgc    180

ttctcgtcta ttgagaagtg atttttttat ctttctgtat ccataatggg gaattttttg    240

tttttatata atagtaatac ggaaaagata gttctaattc cctaaaaaaa tacacttcat    300

atatttttag ttttggttat cgatttggca tggaatttgc atgtgtaaga agggtttatc    360

agacatttta attgtcagga aaagaaaact agttacatac tataaaacta ttttacatta    420

tctactagaa tcaaaaaatt ttaataatat agtaaaatgt agttgacctt cctagtttta    480

gaatataaaa taaatcttgc taatagtttg aaaattctaa aatggagggg tttactgtac    540

aatgaagacg aagtggctaa tatcagtcat aatcctattt gttttcattt ttcctcaaaa    600

tctagttttt gctggtacta gtagtacggc aatcgcacag gaaggagcag ctccggccgc    660

tatattacat ccagagaaat ggcctcgacc tgcgacacaa cgacttattg acccggcagt    720

tgaaaaaaga gttgatgctc tgttaaaaca gttatctgtt gaagaaaaag tagggcaagt    780

tatacagggt gatattggga caattacacc agaagacctg cgcaaatatc cactaggttc    840

tattttagcc ggaggaaaca gcggcccgaa tggagatgat cgtgctcctc caaaggagtg    900

gcttgatcta gctgatgctt tttaccgtgt aagtttagaa aaacggccag gccatacccc    960

gataccagtg ctttttggca ttgatgcagt tcatggacat ggcaatatcg ggtctgcgac   1020

aattttccct cacaatattg cacttggagc aacccatgat ccagaacttc tacgaagaat   1080

tggtgaggta acagctgttg aaatggctgc tacgggaatt gattggacat ttgcgcctgc   1140

actgtctgtt gtgagagatg atcgatgggg acgaacatat gaaggcttct cagaagatcc   1200

agaaattgta gctgcgtatt cagcagcaat tgtggaaggc gtacagggta aatttggttc   1260

taaggatttt atggcgccgg gtcgcattgt agcgtcagca aagcacttct tagctgatgg   1320

tggaacagat caaggacgcg atcagggaga tgcacgcatt tcagaagacg aactaattcg   1380

cattcataat gctggatacc ctcctgcgat tgacgcagga gtgctgacag taatggcttc   1440

tttttcatcc tggcagggga ttaaacacca tggccataaa caacttttaa cagatgtatt   1500

aaaaggacaa atggggttta atggatttat tgtgggggat tggaatgctc atgaccaagt   1560

accgggctgt actaaattta attgtccaac atctcttatt gcgggtttag atatgtatat   1620

ggccgccgat tcctggaagc agctgtacga aaacacctta gcacaagtga aagatggtac   1680

tattcctatg gcacgtctag atgatgccgt aagacgaatc ttgcgagtca aggtgttggc   1740

tggcttattc gagaaacctg cgccaaaaga tcgtccgggg ttaccaggcc ttgaaacact   1800

aggatcacct gaacatagag ccgtaggccg tgaagctgtt cgaaaaagcc tagttcttct   1860

taaaaatgat aaaggtaccc ttccactgtc accaaaggct agagtattag ttgcaggtga   1920

cggagcagat aatattggca aacagtcggg gggctggacg attagttggc aaggaactgg   1980

aaaccgtaac gatgaatttc cgggtgctac atccatttta ggtgggattc gagacgctgt   2040

agctgatgca ggagggtccg tagaatttga tgtagcgggt cagtataaaa caaaacctga   2100

tgtagctatt gttgtttttg gcgaagaacc ttatgctgag tttcagggag atgtggagac   2160

actggaatat caaccagatc aaaaacaaga tcttgctcta ctcaagaaac tgaaagatca   2220

gggaatacct gttgttgctg ttttcctttc tggacgaccg atgtgggtta atcctgaact   2280

taatgccagc gatgctttcg ttgcagcatg gcttcctggc acagaaggtg gcggtgtggc   2340

ggatgtattg tttacagaca aagcgggaaa agtacaacat gattttgcag gaaaattgtc   2400

atatagttgg ccgcgtacgg cagcccagac aacagttaac cgtggtgatg cagattataa   2460

tccgttattt gcgtatggtt acggtttaac gtacaaagat aaatcgaaag tgggcactct   2520

acctgaagaa agtggagtac cggctgaagc gcgacagaat gcagggattt attttcgcgc   2580

aggggcgctg agattaccag gaaggtttct gtgatcatga gcat                    2624


<210>  9
<211>  690
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence of translation of inhA/celA construct

<400>  9

Met Lys Thr Lys Trp Leu Ile Ser Val Ile Ile Leu Phe Val Phe Ile 
1               5                   10                  15      


Phe Pro Gln Asn Leu Val Phe Ala Gly Thr Ser Ser Thr Ala Ile Ala 
            20                  25                  30          


Gln Glu Gly Ala Ala Pro Ala Ala Ile Leu His Pro Glu Lys Trp Pro 
        35                  40                  45              


Arg Pro Ala Thr Gln Arg Leu Ile Asp Pro Ala Val Glu Lys Arg Val 
    50                  55                  60                  


Asp Ala Leu Leu Lys Gln Leu Ser Val Glu Glu Lys Val Gly Gln Val 
65                  70                  75                  80  


Ile Gln Gly Asp Ile Gly Thr Ile Thr Pro Glu Asp Leu Arg Lys Tyr 
                85                  90                  95      


Pro Leu Gly Ser Ile Leu Ala Gly Gly Asn Ser Gly Pro Asn Gly Asp 
            100                 105                 110         


Asp Arg Ala Pro Pro Lys Glu Trp Leu Asp Leu Ala Asp Ala Phe Tyr 
        115                 120                 125             


Arg Val Ser Leu Glu Lys Arg Pro Gly His Thr Pro Ile Pro Val Leu 
    130                 135                 140                 


Phe Gly Ile Asp Ala Val His Gly His Gly Asn Ile Gly Ser Ala Thr 
145                 150                 155                 160 


Ile Phe Pro His Asn Ile Ala Leu Gly Ala Thr His Asp Pro Glu Leu 
                165                 170                 175     


Leu Arg Arg Ile Gly Glu Val Thr Ala Val Glu Met Ala Ala Thr Gly 
            180                 185                 190         


Ile Asp Trp Thr Phe Ala Pro Ala Leu Ser Val Val Arg Asp Asp Arg 
        195                 200                 205             


Trp Gly Arg Thr Tyr Glu Gly Phe Ser Glu Asp Pro Glu Ile Val Ala 
    210                 215                 220                 


Ala Tyr Ser Ala Ala Ile Val Glu Gly Val Gln Gly Lys Phe Gly Ser 
225                 230                 235                 240 


Lys Asp Phe Met Ala Pro Gly Arg Ile Val Ala Ser Ala Lys His Phe 
                245                 250                 255     


Leu Ala Asp Gly Gly Thr Asp Gln Gly Arg Asp Gln Gly Asp Ala Arg 
            260                 265                 270         


Ile Ser Glu Asp Glu Leu Ile Arg Ile His Asn Ala Gly Tyr Pro Pro 
        275                 280                 285             


Ala Ile Asp Ala Gly Val Leu Thr Val Met Ala Ser Phe Ser Ser Trp 
    290                 295                 300                 


Gln Gly Ile Lys His His Gly His Lys Gln Leu Leu Thr Asp Val Leu 
305                 310                 315                 320 


Lys Gly Gln Met Gly Phe Asn Gly Phe Ile Val Gly Asp Trp Asn Ala 
                325                 330                 335     


His Asp Gln Val Pro Gly Cys Thr Lys Phe Asn Cys Pro Thr Ser Leu 
            340                 345                 350         


Ile Ala Gly Leu Asp Met Tyr Met Ala Ala Asp Ser Trp Lys Gln Leu 
        355                 360                 365             


Tyr Glu Asn Thr Leu Ala Gln Val Lys Asp Gly Thr Ile Pro Met Ala 
    370                 375                 380                 


Arg Leu Asp Asp Ala Val Arg Arg Ile Leu Arg Val Lys Val Leu Ala 
385                 390                 395                 400 


Gly Leu Phe Glu Lys Pro Ala Pro Lys Asp Arg Pro Gly Leu Pro Gly 
                405                 410                 415     


Leu Glu Thr Leu Gly Ser Pro Glu His Arg Ala Val Gly Arg Glu Ala 
            420                 425                 430         


Val Arg Lys Ser Leu Val Leu Leu Lys Asn Asp Lys Gly Thr Leu Pro 
        435                 440                 445             


Leu Ser Pro Lys Ala Arg Val Leu Val Ala Gly Asp Gly Ala Asp Asn 
    450                 455                 460                 


Ile Gly Lys Gln Ser Gly Gly Trp Thr Ile Ser Trp Gln Gly Thr Gly 
465                 470                 475                 480 


Asn Arg Asn Asp Glu Phe Pro Gly Ala Thr Ser Ile Leu Gly Gly Ile 
                485                 490                 495     


Arg Asp Ala Val Ala Asp Ala Gly Gly Ser Val Glu Phe Asp Val Ala 
            500                 505                 510         


Gly Gln Tyr Lys Thr Lys Pro Asp Val Ala Ile Val Val Phe Gly Glu 
        515                 520                 525             


Glu Pro Tyr Ala Glu Phe Gln Gly Asp Val Glu Thr Leu Glu Tyr Gln 
    530                 535                 540                 


Pro Asp Gln Lys Gln Asp Leu Ala Leu Leu Lys Lys Leu Lys Asp Gln 
545                 550                 555                 560 


Gly Ile Pro Val Val Ala Val Phe Leu Ser Gly Arg Pro Met Trp Val 
                565                 570                 575     


Asn Pro Glu Leu Asn Ala Ser Asp Ala Phe Val Ala Ala Trp Leu Pro 
            580                 585                 590         


Gly Thr Glu Gly Gly Gly Val Ala Asp Val Leu Phe Thr Asp Lys Ala 
        595                 600                 605             


Gly Lys Val Gln His Asp Phe Ala Gly Lys Leu Ser Tyr Ser Trp Pro 
    610                 615                 620                 


Arg Thr Ala Ala Gln Thr Thr Val Asn Arg Gly Asp Ala Asp Tyr Asn 
625                 630                 635                 640 


Pro Leu Phe Ala Tyr Gly Tyr Gly Leu Thr Tyr Lys Asp Lys Ser Lys 
                645                 650                 655     


Val Gly Thr Leu Pro Glu Glu Ser Gly Val Pro Ala Glu Ala Arg Gln 
            660                 665                 670         


Asn Ala Gly Ile Tyr Phe Arg Ala Gly Ala Leu Arg Leu Pro Gly Arg 
        675                 680                 685             


Phe Leu 
    690 


<210>  10
<211>  1912
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence including inhA/cbhII construct

<400>  10
gagctggaaa agaaagggaa atccgtgagt tatgaagggg atttcttgtt taaaattgat     60

gaaaaacctt atgaaatcgc agcgcgcaat atgtctattt ggagcatgtc gctctatcat    120

atcttgctgc agcgtcaaaa agaacattct taaaatttat ttgtatacag agattatcgc    180

ttctcgtcta ttgagaagtg atttttttat ctttctgtat ccataatggg gaattttttg    240

tttttatata atagtaatac ggaaaagata gttctaattc cctaaaaaaa tacacttcat    300

atatttttag ttttggttat cgatttggca tggaatttgc atgtgtaaga agggtttatc    360

agacatttta attgtcagga aaagaaaact agttacatac tataaaacta ttttacatta    420

tctactagaa tcaaaaaatt ttaataatat agtaaaatgt agttgacctt cctagtttta    480

gaatataaaa taaatcttgc taatagtttg aaaattctaa aatggagggg tttactgtac    540

aatgaaaaag aaaaaacagg ctttaaaggt attattatca gttggtatcc tttcttcatc    600

atttgctttt gcacatacga gcagtgccgc gactagtatg gggcctgctg cacctactgc    660

acgtgtggat aatccttatg taggcgcgac aatgtacgta aatccagaat ggtcagctct    720

tgctgcttcg gaaccaggtg gtgatcgtgt tgcagatcaa cctacggctg tttggttaga    780

tcgtattgca actattgaag gtgttgatgg aaaaatggga ttacgagaac atcttgatga    840

agcgttacaa caaaaaggaa gcggagaact tgtggtacag ttagtaattt atgatttacc    900

tggtcgtgat tgcgcggctc ttgctagtaa tggtgaatta ggtcctgatg aattagatcg    960

atataaaagc gaatatattg atccgattgc agacatttta tcggattcca aatatgaagg   1020

acttcgtatt gttacggtta ttgaaccaga cagcttacct aatttagtaa caaacgcagg   1080

tggtacagat acaacgacag aagcatgtac tactatgaaa gcaaacggta attatgaaaa   1140

aggggtatcg tatgcgcttt ctaaattagg tgcaattccg aacgtataca actatattga   1200

tgctgctcat catggatggt taggatggga cacaaattta gggccatccg tacaggaatt   1260

ttataaagtg gcaacatcaa atggcgcatc cgttgatgat gtggcgggat ttgcagtcaa   1320

tacagctaat tattcaccta ctgtagaacc ttattttacg gtttcagata cggtgaatgg   1380

gcagacggta cgtcaatcta aatgggttga ctggaatcaa tacgtagatg aacaaagtta   1440

tgcgcaggct ttacgaaacg aagctgtcgc cgctggattt aatagcgata ttggtgtgat   1500

tattgataca tcccgaaatg gatggggtgg ttcagatcgc ccttcagggc ctggccctca   1560

aacttccgta gatgcttatg tagatggatc acgaattgat cgtcgcgttc atgtaggaaa   1620

ttggtgtaat cagtctggag caggcttagg tgaaagacca acagcagcac cagctagcgg   1680

gattgatgca tatacatgga ttaaaccgcc gggcgaatct gatggaaatt cagctccggt   1740

tgataatgac gaaggaaaag gatttgacca aatgtgtgat cctagctacc agggaaacgc   1800

tcgcaatggc tacaatcctt caggagcgtt acctgatgca ccattaagtg gacaatggtt   1860

ttcggcacaa tttcgtgaat taatgcaaaa tgcatatcct ccattatctt ga           1912


<210>  11
<211>  456
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide sequence of translation of inhA/cbhII construct

<400>  11

Met Lys Lys Lys Lys Gln Ala Leu Lys Val Leu Leu Ser Val Gly Ile 
1               5                   10                  15      


Leu Ser Ser Ser Phe Ala Phe Ala His Thr Ser Ser Ala Ala Thr Ser 
            20                  25                  30          


Met Gly Pro Ala Ala Pro Thr Ala Arg Val Asp Asn Pro Tyr Val Gly 
        35                  40                  45              


Ala Thr Met Tyr Val Asn Pro Glu Trp Ser Ala Leu Ala Ala Ser Glu 
    50                  55                  60                  


Pro Gly Gly Asp Arg Val Ala Asp Gln Pro Thr Ala Val Trp Leu Asp 
65                  70                  75                  80  


Arg Ile Ala Thr Ile Glu Gly Val Asp Gly Lys Met Gly Leu Arg Glu 
                85                  90                  95      


His Leu Asp Glu Ala Leu Gln Gln Lys Gly Ser Gly Glu Leu Val Val 
            100                 105                 110         


Gln Leu Val Ile Tyr Asp Leu Pro Gly Arg Asp Cys Ala Ala Leu Ala 
        115                 120                 125             


Ser Asn Gly Glu Leu Gly Pro Asp Glu Leu Asp Arg Tyr Lys Ser Glu 
    130                 135                 140                 


Tyr Ile Asp Pro Ile Ala Asp Ile Leu Ser Asp Ser Lys Tyr Glu Gly 
145                 150                 155                 160 


Leu Arg Ile Val Thr Val Ile Glu Pro Asp Ser Leu Pro Asn Leu Val 
                165                 170                 175     


Thr Asn Ala Gly Gly Thr Asp Thr Thr Thr Glu Ala Cys Thr Thr Met 
            180                 185                 190         


Lys Ala Asn Gly Asn Tyr Glu Lys Gly Val Ser Tyr Ala Leu Ser Lys 
        195                 200                 205             


Leu Gly Ala Ile Pro Asn Val Tyr Asn Tyr Ile Asp Ala Ala His His 
    210                 215                 220                 


Gly Trp Leu Gly Trp Asp Thr Asn Leu Gly Pro Ser Val Gln Glu Phe 
225                 230                 235                 240 


Tyr Lys Val Ala Thr Ser Asn Gly Ala Ser Val Asp Asp Val Ala Gly 
                245                 250                 255     


Phe Ala Val Asn Thr Ala Asn Tyr Ser Pro Thr Val Glu Pro Tyr Phe 
            260                 265                 270         


Thr Val Ser Asp Thr Val Asn Gly Gln Thr Val Arg Gln Ser Lys Trp 
        275                 280                 285             


Val Asp Trp Asn Gln Tyr Val Asp Glu Gln Ser Tyr Ala Gln Ala Leu 
    290                 295                 300                 


Arg Asn Glu Ala Val Ala Ala Gly Phe Asn Ser Asp Ile Gly Val Ile 
305                 310                 315                 320 


Ile Asp Thr Ser Arg Asn Gly Trp Gly Gly Ser Asp Arg Pro Ser Gly 
                325                 330                 335     


Pro Gly Pro Gln Thr Ser Val Asp Ala Tyr Val Asp Gly Ser Arg Ile 
            340                 345                 350         


Asp Arg Arg Val His Val Gly Asn Trp Cys Asn Gln Ser Gly Ala Gly 
        355                 360                 365             


Leu Gly Glu Arg Pro Thr Ala Ala Pro Ala Ser Gly Ile Asp Ala Tyr 
    370                 375                 380                 


Thr Trp Ile Lys Pro Pro Gly Glu Ser Asp Gly Asn Ser Ala Pro Val 
385                 390                 395                 400 


Asp Asn Asp Glu Gly Lys Gly Phe Asp Gln Met Cys Asp Pro Ser Tyr 
                405                 410                 415     


Gln Gly Asn Ala Arg Asn Gly Tyr Asn Pro Ser Gly Ala Leu Pro Asp 
            420                 425                 430         


Ala Pro Leu Ser Gly Gln Trp Phe Ser Ala Gln Phe Arg Glu Leu Met 
        435                 440                 445             


Gln Asn Ala Tyr Pro Pro Leu Ser 
    450                 455     


<210>  12
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of primer for inhA promoter

<400>  12
ccttcatatg agctggaaaa gaaagggaaa tccgtgag                             38


<210>  13
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of primer for inhA promoter

<400>  13
ggtatgtaca gtaaacccct ccattttaga attttcaaac                           40


<210>  14
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for signal peptide NprM

<400>  14
tactgtacaa tgaaaaagaa aaaacaggct ttaaaggta                            39


<210>  15
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for signal peptide NprM

<400>  15
ccatactagt cgcggcactg ctcgtatgtg caaaagcaaa                           40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for signal peptide PenG

<400>  16
ttactgtaca atgaagacga agtggctaat atcagtcata                           40


<210>  17
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for signal peptide PenG

<400>  17
tactactagt accagcaaaa actagatttt gaggaaaaat                           40


<210>  18
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for construction of truncated promoter in 
       plasmid BmI201

<400>  18
aattaacata tgtttgcatg tgtaagaagg gtttat                               36


<210>  19
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for construction of truncated promoter in 
       plasmid BmI251

<400>  19
aattaacata tgaaaaatac acttcatata ttttta                               36


<210>  20
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for construction of truncated promoter in 
       plasmid BmI301

<400>  20
aattaacata tgttttgttt ttatataata gtaata                               36


<210>  21
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for construction of truncated promoter in 
       plasmid BmI386

<400>  21
aattaacata tgtaaaattt atttgtatac agagat                               36


<210>  22
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA primer for construction of truncated promoter

<400>  22
catatgacga tagattttaa tggacccaa                                       29


<210>  23
<211>  201
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated inhA promoter

<400>  23
tttgcatgtg taagaagggt ttatcagaca ttttaattgt caggaaaaga aaactagtta     60

catactataa aactatttta cattatctac tagaatcaaa aaattttaat aatatagtaa    120

aatgtagttg accttcctag ttttagaata taaaataaat cttgctaata gtttgaaaat    180

tctaaaatgg aggggtttac t                                              201


<210>  24
<211>  251
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated inhA promoter

<400>  24
aaaaatacac ttcatatatt tttagttttg gttatcgatt tggcatggaa tttgcatgtg     60

taagaagggt ttatcagaca ttttaattgt caggaaaaga aaactagtta catactataa    120

aactatttta cattatctac tagaatcaaa aaattttaat aatatagtaa aatgtagttg    180

accttcctag ttttagaata taaaataaat cttgctaata gtttgaaaat tctaaaatgg    240

aggggtttac t                                                         251


<210>  25
<211>  301
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated inhA promoter

<400>  25
ttttgttttt atataatagt aatacggaaa agatagttct aattccctaa aaaaatacac     60

ttcatatatt tttagttttg gttatcgatt tggcatggaa tttgcatgtg taagaagggt    120

ttatcagaca ttttaattgt caggaaaaga aaactagtta catactataa aactatttta    180

cattatctac tagaatcaaa aaattttaat aatatagtaa aatgtagttg accttcctag    240

ttttagaata taaaataaat cttgctaata gtttgaaaat tctaaaatgg aggggtttac    300

t                                                                    301


<210>  26
<211>  386
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of truncated inhA promoter

<400>  26
taaaatttat ttgtatacag agattatcgc ttctcgtcta ttgagaagtg atttttttat     60

ctttctgtat ccataatggg gaattttttg tttttatata atagtaatac ggaaaagata    120

gttctaattc cctaaaaaaa tacacttcat atatttttag ttttggttat cgatttggca    180

tggaatttgc atgtgtaaga agggtttatc agacatttta attgtcagga aaagaaaact    240

agttacatac tataaaacta ttttacatta tctactagaa tcaaaaaatt ttaataatat    300

agtaaaatgt agttgacctt cctagtttta gaatataaaa taaatcttgc taatagtttg    360

aaaattctaa aatggagggg tttact                                         386


<210>  27
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  27
tataaaataa ggcatgctaa tagtttgaaa atttaaaatg gaggggcgaa gtgtacaatg     60


<210>  28
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  28
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggctc gatgtacaat     60

g                                                                     61


<210>  29
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  29
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggctc gatgtacaat     60

g                                                                     61


<210>  30
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  30
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggttt atggaccaat     60

g                                                                     61


<210>  31
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  31
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggcat tatgtacaat     60

g                                                                     61


<210>  32
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  32
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggtcc tatgtacaat     60

g                                                                     61


<210>  33
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  33
tataaaataa ggcatgctaa tagtttgaaa atttaagaga tggagggatt cgtatg         56


<210>  34
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  34
tataaaataa ggcatgctaa tagtttgaaa atttgatatt tggagggaat aaaatg         56


<210>  35
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  35
tataaaataa ggcatgctaa tagtttgaaa attatgtagg agggccatac tctatg         56


<210>  36
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  36
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggttt atgggacaat     60

g                                                                     61


<210>  37
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  37
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggcaa catgtacaat     60

g                                                                     61


<210>  38
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  38
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggtct tatgtacaat     60

g                                                                     61


<210>  39
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  39
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggttt actccatgat     60

g                                                                     61


<210>  40
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  40
tataaaataa ggcatgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  41
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  41
tataaaatat aggctgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  42
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  42
tataaaataa atcttgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  43
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  43
tataaaatag ggtctgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  44
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  44
tataaaataa atcaatagtt tgaaaattct aaaatggagg ggtttactgt acaatg         56


<210>  45
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  45
tataaaataa tgactgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  46
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  46
tataaaataa aggcggctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  47
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  47
tataaaataa atctgcctca tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  48
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  48
tataaaataa atctatttta tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  49
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  49
tataaaataa atctccccta tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  50
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  50
tataaaatac ggggtgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  51
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  51
tataaaataa tcgatgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  52
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  52
tataaaataa atctcctgta tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  53
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  53
tataaaataa atcttcgcca tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  54
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  54
tataaaataa atctccccaa tagtttgaaa attctaaatg gaggggttta ctgtacaatg     60


<210>  55
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  55
tataaaatac tcagtgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  56
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  56
tataaaatac tcagtgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  57
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  57
tataaaataa atcttccaat agtttgaaaa ttctaaaatg gaggggttta ctgtacaatg     60


<210>  58
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  58
tataaaatat tcggtgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  59
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  59
tataaaatac aactgctaat agtttgaaaa ttctaaaatg gaggggttta ctgtacaatg     60


<210>  60
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  60
tataaaataa tccgtgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  61
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  61
tataaaataa atctccttga tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  62
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  62
tataaaatac acattgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


<210>  63
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence of variant of inhA promoter

<400>  63
tataaaatac caattgctaa tagtttgaaa attctaaaat ggaggggttt actgtacaat     60

g                                                                     61


