                         SEQUENCE LISTING

<110>  Medical College of Wisconsin
 
<120>  ENGINEERED BOTULINUM NEUROTOXIN

<130>  650053.00177

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  4017
<212>  DNA
<213>  Clostridium botulinum

<400>  1
gaattcaagt agtagataat aaaaataatg ccacagattt ttattattaa taatgatata       60

tttatctcta actgtttaac tttaacttat aacaatgtaa atatatattt gtctataaaa      120

aatcaagatt acaattgggt tatatgtgat cttaatcatg atataccaaa aaagtcatat      180

ctatggatat taaaaaatat ataaatttaa aattaggaga tgctgtatat gccaaaaatt      240

aatagtttta attataatga tcctgttaat gatagaacaa ttttatatat taaaccaggc      300

ggttgtcaag aattttataa atcatttaat attatgaaaa atatttggat aattccagag      360

agaaatgtaa ttggtacaac cccccaagat tttcatccgc ctacttcatt aaaaaatgga      420

gatagtagtt attatgaccc taattattta caaagtgatg aagaaaagga tagattttta      480

aaaatagtca caaaaatatt taatagaata aataataatc tttcaggagg gattttatta      540

gaagaactgt caaaagctaa tccatattta gggaatgata atactccaga taatcaattc      600

catattggtg atgcatcagc agttgagatt aaattctcaa atggtagcca agacatacta      660

ttacctaatg ttattataat gggagcagag cctgatttat ttgaaactaa cagttccaat      720

atttctctaa gaaataatta tatgccaagc aatcaccgtt ttggatcaat agctatagta      780

acattctcac ctgaatattc ttttagattt aatgataatt gtatgaatga atttattcaa      840

gatcctgctc ttacattaat gcatgaatta atacattcat tacatggact atatggggct      900

aaagggatta ctacaaagta tactataaca caaaaacaaa atcccctaat aacaaatata      960

agaggtacaa atattgaaga attcttaact tttggaggta ctgatttaaa cattattact     1020

agtgctcagt ccaatgatat ctatactaat cttctagctg attataaaaa aatagcgtct     1080

aaacttagca aagtacaagt atctaatcca ctacttaatc cttataaaga tgtttttgaa     1140

gcaaagtatg gattagataa agatgctagc ggaatttatt cggtaaatat aaacaaattt     1200

aatgatattt ttaaaaaatt atacagcttt acggaatttg atttacgaac taaatttcaa     1260

gttaaatgta ggcaaactta tattggacag tataaatact tcaaactttc aaacttgtta     1320

aatgattcta tttataatat atcagaaggc tataatataa ataatttaaa ggtaaatttt     1380

agaggacaga atgcaaattt aaatcctaga attattacac caattacagg tagaggacta     1440

gtaaaaaaaa tcattagatt ttgtaaaaat attgtttctg taaaaggcat aaggaaatca     1500

atatgtatcg aaataaataa tggtgagtta ttttttgtgg cttccgagaa tagttataat     1560

gatgataata taaatactcc taaagaaatt gacgatacag taacttcaaa taataattat     1620

gaaaatgatt tagatcaggt tattttaaat tttaatagtg aatcagcacc tggactttca     1680

gatgaaaaat taaatttaac tatccaaaat gatgcttata taccaaaata tgattctaat     1740

ggaacaagtg atatagaaca acatgatgtt aatgaactta atgtattttt ctatttagat     1800

gcacagaaag tgcccgaagg tgaaaataat gtcaatctca cctcttcaat tgatacagca     1860

ttattagaac aacctaaaat atatacattt ttttcatcag aatttattaa taatgtcaat     1920

aaacctgtgc aagcagcatt atttgtaagc tggatacaac aagtgttagt agattttact     1980

actgaagcta accaaaaaag tactgttgat aaaattgcag atatttctat agttgttcca     2040

tatataggtc ttgctttaaa tataggaaat gaagcacaaa aaggaaattt taaagatgca     2100

cttgaattat taggagcagg tattttatta gaatttgaac ccgagctttt aattcctaca     2160

attttagtat tcacgataaa atctttttta ggttcatctg ataataaaaa taaagttatt     2220

aaagcaataa ataatgcatt gaaagaaaga gatgaaaaat ggaaagaagt atatagtttt     2280

atagtatcga attggatgac taaaattaat acacaattta ataaaagaaa agaacaaatg     2340

tatcaagctt tacaaaatca agtaaatgca attaaaacaa taatagaatc taagtataat     2400

agttatactt tagaggaaaa aaatgagctt acaaataaat atgatattaa gcaaatagaa     2460

aatgaactta atcaaaaggt ttctatagca atgaataata tagacaggtt cttaactgaa     2520

agttctatat cctatttaat gaaaataata aatgaagtaa aaattaataa attaagagaa     2580

tatgatgaga atgtcaaaac gtatttattg aattatatta tacaacatgg atcaatcttg     2640

ggagagagtc agcaagaact aaattctatg gtaactgata ccctaaataa tagtattcct     2700

tttaagcttt cttcttatac agatgataaa attttaattt catattttaa taaattcttt     2760

aagagaatta aaagtagttc agttttaaat atgagatata aaaatgataa atacgtagat     2820

acttcaggat atgattcaaa tataaatatt aatggagatg tatataaata tccaactaat     2880

aaaaatcaat ttggaatata taatgataaa cttagtgaag ttaatatatc tcaaaatgat     2940

tacattatat atgataataa atataaaaat tttagtatta gtttttgggt aagaattcct     3000

aactatgata ataagatagt aaatgttaat aatgaataca ctataataaa ttgtatgaga     3060

gataataatt caggatggaa agtatctctt aatcataatg aaataatttg gacattcgaa     3120

gataatcgag gaattaatca aaaattagca tttaactatg gtaacgcaaa tggtatttct     3180

gattatataa ataagtggat ttttgtaact ataactaatg atagattagg agattctaaa     3240

ctttatatta atggaaattt aatagatcaa aaatcaattt taaatttagg taatattcat     3300

gttagtgaca atatattatt taaaatagtt aattgtagtt atacaagata tattggtatt     3360

agatatttta atatttttga taaagaatta gatgaaacag aaattcaaac tttatatagc     3420

aatgaaccta atacaaatat tttgaaggat ttttggggaa attatttgct ttatgacaaa     3480

gaatactatt tattaaatgt gttaaaacca aataacttta ttgataggag aaaagattct     3540

actttaagca ttaataatat aagaagcact attcttttag ctaatagatt atatagtgga     3600

ataaaagtta aaatacaaag agttaataat agtagtacta acgataatct tgttagaaag     3660

aatgatcagg tatatattaa ttttgtagcc agcaaaactc acttatttcc attatatgct     3720

gatacagcta ccacaaataa agagaaaaca ataaaaatat catcatctgg caatagattt     3780

aatcaagtag tagttatgaa ttcagtagga aattgtacaa tgaattttaa aaataataat     3840

ggaaataata ttgggttgtt aggtttcaag gcagatactg tcgttgctag tacttggtat     3900

tatacacata tgagagatca tacaaacagc aatggatgtt tttggaactt tatttctgaa     3960

gaacatggat ggcaagaaaa ataaaaatta gattaaacgg ctaaagtcat aaattcc        4017


<210>  2
<211>  636
<212>  DNA
<213>  Homo sapiens

<400>  2
atggataatc tgtcatcaga agaaattcaa cagagagctc accagattac tgatgagtct       60

ctggaaagta cgaggagaat cctgggttta gccattgagt ctcaggatgc aggaatcaag      120

accatcacta tgctggatga acaaaaggaa caactaaacc gcatagaaga aggcttggac      180

caaataaata aggacatgag agagacagag aagactttaa cagaactcaa caaatgctgt      240

ggcctttgtg tctgcccatg taatagaaca aagaactttg agtctggcaa ggcttataag      300

acaacatggg gagatggtgg agaaaactca ccttgcaatg tagtatctaa acagccaggc      360

ccggtgacaa atggtcagct tcagcaacca acaacaggag cagccagtgg tggatacatt      420

aaacgcataa ctaatgatgc cagagaagat gaaatggaag agaacctgac tcaagtgggc      480

agtatcctgg gaaatctaaa agacatggcc ctgaacatag gcaatgagat tgatgctcaa      540

aatccacaaa taaaacgaat cacagacaag gctgacacca acagagatcg tattgatatt      600

gccaatgcca gagcaaagaa actcattgac agttaa                                636


<210>  3
<211>  2053
<212>  DNA
<213>  Homo sapiens

<400>  3
catctttgat gagggcagag ctcacgttgc attgaagacg aaacctcggg gaggtcaggc       60

gctgtctttc cttccctccc tgctcggcgg ctccaccaca gttgcaacct gcagaggccc      120

ggagaacaca accctcccga gaagcccagg tccagagcca aacccgtcac tgacccccca      180

gcccaggcgc ccagccactc cccaccgcta ccatggccga agacgcagac atgcgcaatg      240

agctggagga gatgcagcga agggctgacc agttggctga tgagtcgctg gaaagcaccc      300

gtcgtatgct gcaactggtt gaagagagta aagatgctgg tatcaggact ttggttatgt      360

tggatgaaca aggagaacaa ctggaacgca ttgaggaagg gatggaccaa atcaataagg      420

acatgaaaga agcagaaaag aatttgacgg acctaggaaa attctgcggg ctttgtgtgt      480

gtccctgtaa caagcttaaa tcaagtgatg cttacaaaaa agcctggggc aataatcagg      540

acggagtggt ggccagccag cctgctcgtg tagtggacga acgggagcag atggccatca      600

gtggcggctt catccgcagg gtaacaaatg atgcccgaga aaatgaaatg gatgaaaacc      660

tagagcaggt gagcggcatc atcgggaacc tccgtcacat ggccctggat atgggcaatg      720

agatcgatac acagaatcgc cagatcgaca ggatcatgga gaaggctgat tccaacaaaa      780

ccagaattga tgaggccaac caacgtgcaa caaagatgct gggaagtggt taagtgtgcc      840

cacccgtgtt ctcctccaaa tgctgtcggg caagatagct ccttcatgct tttctcatgg      900

tattatctag taggtctgca cacataacac acatcagtcc acccccattg tgaatgttgt      960

cctgtgtcat ctgtcagctt cccaacaata ctttgtgtct tttgttctct cttggtctct     1020

ttctttccaa aggttgtaca tagtggtcat ttggtggctc taactccttg atgtcttgag     1080

tttcattttt cattttctct cctcggtggc atttgctgaa taacaacaat ttaggaatgc     1140

tcaatgtgct gttgattctt tcaatccaca gtattgttct tgtaaaactg tgacattcca     1200

cagagttact gccacggtcc tttgagtgtc aggctctgaa tctctcaaaa tgtgccgtct     1260

ttggttcctc atggctgtta tctgtcttta tgatttcatg attagacaat gtggaattac     1320

ataacaggca ttgcactaaa agtgatgtga tttatgcatt tatgcatgag aactaaatag     1380

atttttagat tcctacttaa acaaaaactt tccatgacag tagcatactg atgagacaac     1440

acacacacac acaaaacaac agcaacaaca acagaacaac aacaaagcat gctcagtatt     1500

gagacactgt caagattaag ttataccagc aaaagtgcag tagtgtcact tttttcctgt     1560

caatatatag agacttctaa atcataatca tcctttttta aaaaaaagaa ttttaaaaaa     1620

gatggatttg acacactcac catttaatca tttccagcaa aatatatgtt tggctgaaat     1680

tatgtcaaat ggatgtaata tagggtttgt ttgctgcttt tgatggctac gttttggaga     1740

gagcaatctt gctgtgaaac agtgtggatg taaattttat aaggctgact cttactaacc     1800

accatttccc ctgtggtttg ttatcagtac aattctttgt tgcttaatct agagctatgc     1860

acaccaaatt gctgagatgt ttagtagctg ataaagaaac cttttaaaaa aataatataa     1920

atgaatgaaa tataaactgt gagataaata tcattatagc atgtaatatt aaattcctcc     1980

tgtctcctct gtcagtttgt gaagtgattg acattttgta gctagtttaa aattattaaa     2040

aattatagac tcc                                                        2053


<210>  4
<211>  1019
<212>  DNA
<213>  Homo sapiens

<400>  4
tctgtttccc agaccgagag ccgcgccggc accatgtcag cttaccctaa aagctacaat       60

ccgttcgacg acgacgggga ggacgaaggc gcccggccgg ccccttggag ggacgcccga      120

gacctccccg acgggcccga cgcgcccgcg gacaggcagc agtacttgcg gcaggaggtc      180

ctccgcaggg ctgaggccac ggccgccagc accagcaggt ccctggccct catgtacgag      240

tccgagaagg ttggggtcgc ctcttccgag gagctcgccc gtcagcgagg agtcctggag      300

cgcacagaga agatggtgga caagatggac caagatttga agatcagcca gaaacacatc      360

aatagcatta agagcgtgtt tggggggctg gtcaattact tcaaatccaa accagtagag      420

accccacctg aacagaatgg caccctcacc tcccagccca acaacagatt gaaagaagct      480

ataagtacaa gtaaagaaca ggaagcaaag taccaggcca gccacccaaa ccttagaaag      540

ctggatgata cagaccctgt ccccagaggg gctggttctg ccatgagtac tgatgcttac      600

ccaaagaacc cacaccttcg agcctatcac cagaagatcg acagcaacct agatgagctg      660

tccatgggac tgggtcgtct gaaggacata gccctgggga tgcagacaga aattgaggag      720

caagatgaca ttcttgaccg gctgacaacc aaagtggaca agttagatgt caacataaaa      780

agcacagaaa gaaaagttcg acaactctga agacagacgg atttccactc tattgtgatg      840

aaaagatttg aaagatcttt ttttgaactt ccaagaaatt tcatttacta ttttagtatg      900

taaattaatg tgtgtttgca aatgttataa tagagtaggt cttaagacat ttttgctgtt      960

ataaggaagt gtttgtccca cattttccta gggttaacac ctcaccaagt tcttccagc      1019


<210>  5
<211>  1925
<212>  DNA
<213>  Homo sapiens

<400>  5
ggcgccgagc ggccgaggcg ccgcggtcgg ctctgggact cgtctggcgt ccctcagagg       60

cagaagaggc ctggaccttg gcgcacacag acccaggaac agatgagcag ggatgtctgc      120

atccacacct ggccgtgcac ctactacctg gagcccaaga ggcgatgggt tactggacag      180

ctgtccttaa catcgctgtc gctcaggttc atgactgaca gcactggaga gattctggtc      240

agcttccccc tctccagcat agttgagatc aagaaggagg cttcacattt tatcttcagc      300

tccatcacca tcctggagaa gcgccatgcc aagcactggt tcagctccct gcggccaagt      360

cgaaatgtgg tcttcagcat catcgagcat ttctggaggg agctgctgct gtctcagcct      420

ggagccgtgg cagacgcatc tgtcccaagg acccggggcg aggagctgac gggactcatg      480

gctggatccc agaaacgcct ggaggacacg gcgagggtcc tgcaccacca gggccagcag      540

ctggacagcg tcatgagagg cctggacaag atggagtcag acctggaggt ggcggacaga      600

ttgctgacag aactggaatc tcctgcttgg tggcccttta gctccaagct ttggaagaca      660

ccaccggaaa caaagcccag ggaagatgtc tccatgacca gttgtgaacc ctttgggaaa      720

gaagggatac tgataaaaat tcctgctgtt atttcccaca gaacagagtc tcacgttaaa      780

ccagggaggc tcaccgtcct tgtgtctggg ttggaaatac atgactccag ttctttgctc      840

atgcacaggt ttgaaagaga agacgtggac gacatcaagg tccactcacc ttacgaaatt      900

agcatccgcc agcggtttat tggaaagcca gacatggcct atcgtttgat atctgccaag      960

atgccagagg ttatccccat tttagaagtg cagttcagca agaagatgga gctgttagaa     1020

gatgcattgg tgctcagaag cgcaagaacc tcttcccccg cagagaagag ctgctcagtc     1080

tggcatgcag catctgggct gatgggccgt accctgcacc gtgagccacc cgcaggagac     1140

caggagggca cagcactgca cctgcagaca agcctgccag ccctttctga ggcagatacc     1200

caggaactaa cccagatcct gaggaggatg aaggggctgg ccctggaggc cgagagtgag     1260

ctggagagac aagacgaagc cctggatggc gttgcagcag ctgtggacag ggcaaccttg     1320

accatcgaca agcacaacag gcggatgaag aggctgacct aggggcagaa cgtccctgca     1380

ttcctgtctc accctgcaca tcccgctgag atggagggct gggcggcagt gccagggctg     1440

cagaggcctg tggccctccg gagtggtctt cctctggatg gggctgctac tgtggggctg     1500

cttctgcacc aggggcctcc ccaggtgtgc accatgcctg cctcccactt ggctgtccct     1560

gctgctgggc aggacccggc cacatgttct gcggatgctg cagaagtgtg gaccatggcg     1620

ggaccccaag gacacttggc acaggcctgg aagaggccgc cctcgtcttg tctcggctcc     1680

ctttcatgga cagactggcc ttcttagctg tactataaat ttgtgagtga agttagagcc     1740

cagctcactt agccagctca ctttgagggc atcctataaa cacccaactg ttcttttatc     1800

gtctcggttt tagccaaaag tgaaattagc atgactgcat ctttcaaaca aaaatattga     1860

tttctgcttt tagggccccg tttccatcca gaaataaagg gaaatgctgg aaaaaaaaaa     1920

aaaaa                                                                 1925


<210>  6
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  6

Ile Lys Arg Ile Thr Asp Lys Ala Asp Thr Asn Arg Asp Arg Ile Asp 
1               5                   10                  15      


Ile Ala Asn Ala Arg Ala Lys Lys Leu Ile Asp Ser 
            20                  25              


<210>  7
<211>  1250
<212>  PRT
<213>  Clostridium botulinum

<400>  7

Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg Thr 
1               5                   10                  15      


Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser Phe 
            20                  25                  30          


Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile Gly 
        35                  40                  45              


Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly Asp 
    50                  55                  60                  


Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys Asp 
65                  70                  75                  80  


Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn Asn 
                85                  90                  95      


Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro Tyr 
            100                 105                 110         


Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp Ala 
        115                 120                 125             


Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu Leu 
    130                 135                 140                 


Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr Asn 
145                 150                 155                 160 


Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His Arg 
                165                 170                 175     


Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe Arg 
            180                 185                 190         


Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu Thr 
        195                 200                 205             


Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala Lys 
    210                 215                 220                 


Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu Ile 
225                 230                 235                 240 


Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly Gly 
                245                 250                 255     


Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr Thr 
            260                 265                 270         


Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys Val 
        275                 280                 285             


Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu Ala 
    290                 295                 300                 


Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn Ile 
305                 310                 315                 320 


Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu Phe 
                325                 330                 335     


Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile Gly 
            340                 345                 350         


Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile Tyr 
        355                 360                 365             


Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe Arg 
    370                 375                 380                 


Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr Gly 
385                 390                 395                 400 


Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val Ser 
                405                 410                 415     


Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly Glu 
            420                 425                 430         


Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile Asn 
        435                 440                 445             


Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr Glu 
    450                 455                 460                 


Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala Pro 
465                 470                 475                 480 


Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala Tyr 
                485                 490                 495     


Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His Asp 
            500                 505                 510         


Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val Pro 
        515                 520                 525             


Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala Leu 
    530                 535                 540                 


Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile Asn 
545                 550                 555                 560 


Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile Gln 
                565                 570                 575     


Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr Val 
            580                 585                 590         


Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu Ala 
        595                 600                 605             


Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala Leu 
    610                 615                 620                 


Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu Leu 
625                 630                 635                 640 


Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser Ser 
                645                 650                 655     


Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys Glu 
            660                 665                 670         


Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn Trp 
        675                 680                 685             


Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met Tyr 
    690                 695                 700                 


Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu Ser 
705                 710                 715                 720 


Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn Lys 
                725                 730                 735     


Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser Ile 
            740                 745                 750         


Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser Tyr 
        755                 760                 765             


Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu Tyr 
    770                 775                 780                 


Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His Gly 
785                 790                 795                 800 


Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr Asp 
                805                 810                 815     


Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp Asp 
            820                 825                 830         


Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys Ser 
        835                 840                 845             


Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp Thr 
    850                 855                 860                 


Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys Tyr 
865                 870                 875                 880 


Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser Glu 
                885                 890                 895     


Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr Lys 
            900                 905                 910         


Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn Lys 
        915                 920                 925             


Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg Asp 
    930                 935                 940                 


Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile Trp 
945                 950                 955                 960 


Thr Phe Glu Asp Asn Arg Gly Ile Asn Gln Lys Leu Ala Phe Asn Tyr 
                965                 970                 975     


Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe Val 
            980                 985                 990         


Thr Ile Thr Asn Asp Arg Leu Gly  Asp Ser Lys Leu Tyr  Ile Asn Gly 
        995                 1000                 1005             


Asn Leu  Ile Asp Gln Lys Ser  Ile Leu Asn Leu Gly  Asn Ile His 
    1010                 1015                 1020             


Val Ser  Asp Asn Ile Leu Phe  Lys Ile Val Asn Cys  Ser Tyr Thr 
    1025                 1030                 1035             


Arg Tyr  Ile Gly Ile Arg Tyr  Phe Asn Ile Phe Asp  Lys Glu Leu 
    1040                 1045                 1050             


Asp Glu  Thr Glu Ile Gln Thr  Leu Tyr Ser Asn Glu  Pro Asn Thr 
    1055                 1060                 1065             


Asn Ile  Leu Lys Asp Phe Trp  Gly Asn Tyr Leu Leu  Tyr Asp Lys 
    1070                 1075                 1080             


Glu Tyr  Tyr Leu Leu Asn Val  Leu Lys Pro Asn Asn  Phe Ile Asp 
    1085                 1090                 1095             


Arg Arg  Lys Asp Ser Thr Leu  Ser Ile Asn Asn Ile  Arg Ser Thr 
    1100                 1105                 1110             


Ile Leu  Leu Ala Asn Arg Leu  Tyr Ser Gly Ile Lys  Val Lys Ile 
    1115                 1120                 1125             


Gln Arg  Val Asn Asn Ser Ser  Thr Asn Asp Asn Leu  Val Arg Lys 
    1130                 1135                 1140             


Asn Asp  Gln Val Tyr Ile Asn  Phe Val Ala Ser Lys  Thr His Leu 
    1145                 1150                 1155             


Phe Pro  Leu Tyr Ala Asp Thr  Ala Thr Thr Asn Lys  Glu Lys Thr 
    1160                 1165                 1170             


Ile Lys  Ile Ser Ser Ser Gly  Asn Arg Phe Asn Gln  Val Val Val 
    1175                 1180                 1185             


Met Asn  Ser Val Gly Asn Cys  Thr Met Asn Phe Lys  Asn Asn Asn 
    1190                 1195                 1200             


Gly Asn  Asn Ile Gly Leu Leu  Gly Phe Lys Ala Asp  Thr Val Val 
    1205                 1210                 1215             


Ala Ser  Thr Trp Tyr Tyr Thr  His Met Arg Asp His  Thr Asn Ser 
    1220                 1225                 1230             


Asn Gly  Cys Phe Trp Asn Phe  Ile Ser Glu Glu His  Gly Trp Gln 
    1235                 1240                 1245             


Glu Lys  
    1250 


<210>  8
<211>  31
<212>  PRT
<213>  Clostridium botulinum

<400>  8

Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala Lys Gly 
1               5                   10                  15      


Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu Ile 
            20                  25                  30      


