
                                SEQUENCE LISTING

<110> ATYR PHARMA, INC.
      Adams, Ryan A.
      Hong, Fei
      Zhao, Ji
      Piehl, Kristi
      Armour, Eva R.
      D'Arigo, Kenny
      Greene, Leslie Ann
      Merriman, Eve	

<120> COMPOSITIONS AND METHODS COMPRISING
  ASPARTYL-tRNA SYNTHETASES HAVING NON-CANONICAL BIOLOGICAL
  ACTIVITIES

<130> 120161.412PC

<140> PCT                  
<141> 2010-03-31  

<150> US 61/165,194       
<151> 2009-03-31  

<160> 23

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 501
<212> PRT
<213> Homo sapiens

<400> 1
Met Pro Ser Ala Ser Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu
 1               5                  10                  15      
Ile Met Asp Ala Ala Glu Asp Tyr Ala Lys Glu Arg Tyr Gly Ile Ser
            20                  25                  30          
Ser Met Ile Gln Ser Gln Glu Lys Pro Asp Arg Val Leu Val Arg Val
        35                  40                  45              
Arg Asp Leu Thr Ile Gln Lys Ala Asp Glu Val Val Trp Val Arg Ala
    50                  55                  60                  
Arg Val His Thr Ser Arg Ala Lys Gly Lys Gln Cys Phe Leu Val Leu
65                  70                  75                  80  
Arg Gln Gln Gln Phe Asn Val Gln Ala Leu Val Ala Val Gly Asp His
                85                  90                  95      
Ala Ser Lys Gln Met Val Lys Phe Ala Ala Asn Ile Asn Lys Glu Ser
            100                 105                 110         
Ile Val Asp Val Glu Gly Val Val Arg Lys Val Asn Gln Lys Ile Gly
        115                 120                 125             
Ser Cys Thr Gln Gln Asp Val Glu Leu His Val Gln Lys Ile Tyr Val
    130                 135                 140                 
Ile Ser Leu Ala Glu Pro Arg Leu Pro Leu Gln Leu Asp Asp Ala Val
145                 150                 155                 160 
Arg Pro Glu Ala Glu Gly Glu Glu Glu Gly Arg Ala Thr Val Asn Gln
                165                 170                 175     
Asp Thr Arg Leu Asp Asn Arg Val Ile Asp Leu Arg Thr Ser Thr Ser
            180                 185                 190         
Gln Ala Val Phe Arg Leu Gln Ser Gly Ile Cys His Leu Phe Arg Glu
        195                 200                 205             
Thr Leu Ile Asn Lys Gly Phe Val Glu Ile Gln Thr Pro Lys Ile Ile
    210                 215                 220                 
Ser Ala Ala Ser Glu Gly Gly Ala Asn Val Phe Thr Val Ser Tyr Phe
225                 230                 235                 240 
Lys Asn Asn Ala Tyr Leu Ala Gln Ser Pro Gln Leu Tyr Lys Gln Met
                245                 250                 255     
Cys Ile Cys Ala Asp Phe Glu Lys Val Phe Ser Ile Gly Pro Val Phe
            260                 265                 270         
Arg Ala Glu Asp Ser Asn Thr His Arg His Leu Thr Glu Phe Val Gly
        275                 280                 285             
Leu Asp Ile Glu Met Ala Phe Asn Tyr His Tyr His Glu Val Met Glu
    290                 295                 300                 
Glu Ile Ala Asp Thr Met Val Gln Ile Phe Lys Gly Leu Gln Glu Arg
305                 310                 315                 320 
Phe Gln Thr Glu Ile Gln Thr Val Asn Lys Gln Phe Pro Cys Glu Pro
                325                 330                 335     
Phe Lys Phe Leu Glu Pro Thr Leu Arg Leu Glu Tyr Cys Glu Ala Leu
            340                 345                 350         
Ala Met Leu Arg Glu Ala Gly Val Glu Met Gly Asp Glu Asp Asp Leu
        355                 360                 365             
Ser Thr Pro Asn Glu Lys Leu Leu Gly His Leu Val Lys Glu Lys Tyr
    370                 375                 380                 
Asp Thr Asp Phe Tyr Ile Leu Asp Lys Tyr Pro Leu Ala Val Arg Pro
385                 390                 395                 400 
Phe Tyr Thr Met Pro Asp Pro Arg Asn Pro Lys Gln Ser Asn Ser Tyr
                405                 410                 415     
Asp Met Phe Met Arg Gly Glu Glu Ile Leu Ser Gly Ala Gln Arg Ile
            420                 425                 430         
His Asp Pro Gln Leu Leu Thr Glu Arg Ala Leu His His Gly Ile Asp
        435                 440                 445             
Leu Glu Lys Ile Lys Ala Tyr Ile Asp Ser Phe Arg Phe Gly Ala Pro
    450                 455                 460                 
Pro His Ala Gly Gly Gly Ile Gly Leu Glu Arg Val Thr Met Leu Phe
465                 470                 475                 480 
Leu Gly Leu His Asn Val Arg Gln Thr Ser Met Phe Pro Arg Asp Pro
                485                 490                 495     
Lys Arg Leu Thr Pro
            500     


<210> 2
<211> 2322
<212> DNA
<213> Homo sapiens

<400> 2
atctcgagat agccgcagct ctcgcgatct ttctggagcc gcacctccac gcggagtccg 60
agcgcgtgtg ctgagacccc agggtcggga gggcggagac tgggagggag ggagaagccc 120
ctttggcctg ccttacggaa gcctgcgagg gagggtggtg tccactgccc agttccgtgt 180
cccgatgccc agcgccagcg ccagccgcaa gagtcaggag aagccgcggg agatcatgga 240
cgcggcggaa gattatgcta aagagagata tggaatatct tcaatgatac aatcacaaga 300
aaaaccagat cgagttttgg ttcgggttag agacttgaca atacaaaaag ctgatgaagt 360
tgtttgggta cgtgcaagag ttcatacaag cagagctaaa gggaaacagt gcttcttagt 420
cctacgtcag cagcagttta atgtccaggc tcttgtggcg gtgggagacc atgcaagcaa 480
gcagatggtt aaatttgctg ccaacatcaa caaagagagc attgtggatg tagaaggtgt 540
tgtgagaaaa gtgaatcaga aaattggaag ctgtacacag caagacgttg agttacatgt 600
tcagaagatt tatgtgatca gtttggctga accccgtctg cccctgcagc tggatgatgc 660
tgttcggcct gaggcagaag gagaagagga aggaagagct actgttaacc aggatacaag 720
attagacaac agagtcattg atcttaggac atcaactagt caggcagtct tccgtctcca 780
gtctggcatc tgccatctct tccgagaaac tttaattaac aaaggttttg tggaaatcca 840
aactcctaaa attatttcag ctgccagtga aggaggagcc aatgttttta ctgtgtcata 900
ttttaaaaat aatgcatacc tggctcagtc cccacagcta tataagcaaa tgtgcatttg 960
tgctgatttt gagaaggttt tctctattgg accagtattc agagcggaag actctaatac 1020
ccatagacat ctaactgagt ttgttggttt ggacattgaa atggctttta attaccatta 1080
ccacgaagtt atggaagaaa ttgctgacac catggtacaa atattcaaag gacttcaaga 1140
aaggtttcag actgaaattc aaacagtgaa taaacagttc ccatgtgagc cattcaaatt 1200
tttggagcca actctaagac tagaatattg tgaagcattg gctatgctta gggaagctgg 1260
agtcgaaatg ggagatgaag acgatctgag cacaccaaat gaaaagctgt tgggtcattt 1320
ggtaaaggaa aagtatgata cagattttta tattcttgat aaatatccat tggctgtaag 1380
acctttctat accatgcctg acccaagaaa tcccaaacag tccaactctt acgatatgtt 1440
catgagagga gaagaaatat tgtcaggagc tcaaagaata catgatcctc aactgctaac 1500
agagagagct ttacatcatg gaattgattt ggagaaaatt aaggcttaca ttgattcctt 1560
ccgctttgga gcccctcctc atgctggtgg aggcattgga ttggaacgag ttactatgct 1620
gtttctggga ttgcataatg ttcgtcagac ctccatgttc cctcgtgatc ccaaacgact 1680
cactccttaa attcacactt tgccacttaa ctccagtgtg gatgacagag cgagaccctg 1740
cctcaaaaaa aaaaaaaaaa aaagaaagcc acacttattc ttttcagtaa cctgctagtg 1800
cacaggctgt actttaggta cttaaaatat gcactagaat aaatttgcaa ggccctaaaa 1860
tatcactgtt atttttggag taattcagta taggttcgtt taaaagagat ttttataact 1920
tcagacatgc atcagtagga aataacttga gaaattcata tggttatgtt acaaattcat 1980
attctgttac tacagtaaac gttaagagtt ttaaacagtt aagattgtac aatttttctt 2040
cttttctata ttacaagggc cccagtgtta atgtcttaga ttttcagtat ttgaacttat 2100
ttttttaaat tctgtcattg agataagaat aattcaggta gcatctgaaa ttttaatgaa 2160
tgtataattg gcatatcatg gaaaattaac cagaaagtat cagttcttaa aagttatgcc 2220
tagaaattat gtaaagctaa actactggtt agaaagtatt cagtgtaata ttgtattaat 2280
ttgttaaatt ctaaacttga atttcaataa aattttaaag ct                    2322

<210> 3
<211> 32
<212> PRT
<213> Homo sapiens

<400> 3
Met Pro Ser Ala Ser Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu
 1               5                  10                  15      
Ile Met Asp Ala Ala Glu Asp Tyr Ala Lys Glu Arg Tyr Gly Ile Ser
            20                  25                  30          


<210> 4
<211> 32
<212> PRT
<213> Rattus norvegicus

<400> 4
Met Pro Ser Ala Asn Ala Ser Arg Lys Gly Gln Glu Lys Pro Arg Glu
 1               5                  10                  15      
Ile Val Asp Ala Ala Glu Asp Tyr Ala Lys Glu Arg Tyr Gly Val Ser
            20                  25                  30          


<210> 5
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Consensus sequence of the positively charged
      residues of the AspRS amphiphilic helix

<220> 
<221> VARIANT        
<222> 5, 9, 10
<223> Xaa = Any non-positively charged amino acid

<400> 5
Leu Ser Lys Lys Xaa Leu Lys Lys Xaa Xaa Lys
 1               5                  10      


<210> 6
<211> 25
<212> PRT
<213> Anopheles gambiae

<400> 6
Glu Glu Pro Val Gly Ala Gly Ala Glu Ala Thr Ser Lys Lys Ala Ala
 1               5                  10                  15      
Lys Lys Ala Ala Lys Asp Ala Glu Lys
            20                  25  


<210> 7
<211> 27
<212> PRT
<213> Ixodes scapularis

<400> 7
Asp Glu Asn Ala Ala Leu Gly Pro Asp Gly Gln Pro Leu Ser Lys Lys
 1               5                  10                  15      
Ala Leu Lys Lys Gln Ala Lys Glu Gln Glu Lys
            20                  25          


<210> 8
<211> 19
<212> PRT
<213> Lottia gigantea

<400> 8
Asp Gly Lys Pro Thr Ser Lys Lys Gly Leu Lys Lys Gln Gln Lys Glu
 1               5                  10                  15      
Ala Glu Lys
            


<210> 9
<211> 27
<212> PRT
<213> Helobdella robusta

<400> 9
Asn Val Ser Glu Ala Val Gly Gly Asp Asp Lys Pro Thr Ser Lys Lys
 1               5                  10                  15      
Ala Leu Lys Lys Gln Gln Lys Glu Ala Glu Lys
            20                  25          


<210> 10
<211> 23
<212> PRT
<213> Xenopus trapicalis

<400> 10
Ser Ala Ala Ser Glu Glu Gln Ala Gln Ser Lys Lys Ala Leu Lys Lys
 1               5                  10                  15      
Gln Gln Lys Glu Ala Glu Lys
            20              


<210> 11
<211> 26
<212> PRT
<213> Takifugu rubripes

<400> 11
Ser Cys Phe Ser Ala Ala Glu Ala Glu Gln Ala Gln Ser Lys Lys Gly
 1               5                  10                  15      
Leu Lys Lys Gln Gln Lys Glu Ala Glu Lys
            20                  25      


<210> 12
<211> 26
<212> PRT
<213> Tetraodon nigroviridis

<400> 12
Asp Val Lys Gly Ala Ala Glu Glu Glu Gln Ala Gln Ser Lys Lys Gly
 1               5                  10                  15      
Leu Lys Lys Gln Gln Lys Glu Ala Glu Lys
            20                  25      


<210> 13
<211> 27
<212> PRT
<213> Gasterosteus aculeatus

<400> 13
Glu Ala Pro Gly Ala Thr Glu Glu Glu Gln Gln Ala Gln Ser Lys Lys
 1               5                  10                  15      
Gly Leu Lys Lys Gln Gln Lys Glu Ala Glu Lys
            20                  25          


<210> 14
<211> 23
<212> PRT
<213> Gallus gallus

<400> 14
Pro Ser Ala Ala Gly Arg Gly Gln Gly Lys Asp Arg Arg Pro Asp Asn
 1               5                  10                  15      
Glu Glu Gln Pro Ala Ala Asp
            20              


<210> 15
<211> 21
<212> PRT
<213> Bos taurus

<400> 15
Pro Ser Ala Ser Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 16
<211> 20
<212> PRT
<213> Rattus norvegicus

<400> 16
Pro Ser Ala Asn Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Asp Ala Ala Glu
            20  


<210> 17
<211> 21
<212> PRT
<213> Mus musculus

<400> 17
Pro Ser Ala Ser Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 18
<211> 21
<212> PRT
<213> Procavia capensis

<400> 18
Pro Ser Ala Ser Ala Gly Arg Lys Asn Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 19
<211> 21
<212> PRT
<213> Monodephis domestica

<400> 19
Pro Ser Ala Ile Ala Ser Arg Lys Thr Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 20
<211> 21
<212> PRT
<213> Tarius syrichta

<400> 20
Pro Ser Ala Ser Ala Ser Arg Arg Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 21
<211> 21
<212> PRT
<213> Pongo abelii

<400> 21
Pro Ser Ala Ser Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 22
<211> 21
<212> PRT
<213> Pan troglodytes

<400> 22
Pro Ser Ala Asp Ala Ser Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      


<210> 23
<211> 21
<212> PRT
<213> Homo sapiens

<400> 23
Pro Ser Ala Ser Ala Cys Arg Lys Ser Gln Glu Lys Pro Arg Glu Ile
 1               5                  10                  15      
Met Asp Ala Ala Glu
            20      

