                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
       Jansen, Mickel
       Verwaal, Ren
       Maarten, Graaf van de
 
<120>  Dicarboxylic acid production process

<130>  27169-WO-PCT

<150>  EP09157964.9
<151>  2009-04-15

<150>  EP09169412.5
<151>  2009-09-03

<150>  61/239,566
<151>  2009-09-03

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  3148
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct TDH1p-PCKm-TDH1t for expression in S. 
       cerevisiae

<400>  1
ggatcccttc ccttttacag tgcttcggaa aagcacagcg ttgtccaagg gaacaatttt       60

tcttcaagtt aatgcataag aaatatcttt ttttatgttt agctaagtaa aagcagcttg      120

gagtaaaaaa aaaaatgagt aaatttctcg atggattagt ttctcacagg taacataaca      180

aaaaccaaga aaagcccgct tctgaaaact acagttgact tgtatgctaa agggccagac      240

taatgggagg agaaaaagaa acgaatgtat atgctcattt acactctata tcaccatatg      300

gaggataagt tgggctgagc ttctgatcca atttattcta tccattagtt gctgatatgt      360

cccaccagcc aacacttgat agtatctact cgccattcac ttccagcagc gccagtaggg      420

ttgttgagct tagtaaaaat gtgcgcacca caagcctaca tgactccacg tcacatgaaa      480

ccacaccgtg gggccttgtt gcgctaggaa taggatatgc gacgaagacg cttctgctta      540

gtaaccacac cacattttca gggggtcgat ctgcttgctt cctttactgt cacgagcggc      600

ccataatcgc gctttttttt taaaaggcgc gagacagcaa acaggaagct cgggtttcaa      660

ccttcggagt ggtcgcagat ctggagactg gatctttaca atacagtaag gcaagccacc      720

atctgcttct taggtgcatg cgacggtatc cacgtgcaga acaacatagt ctgaagaagg      780

gggggaggag catgttcatt ctctgtagca gtaagagctt ggtgataatg accaaaactg      840

gagtctcgaa atcatataaa tagacaatat attttcacac aatgagattt gtagtacagt      900

tctattctct ctcttgcata aataagaaat tcatcaagaa cttggtttga tatttcacca      960

acacacacaa aaaacagtac ttcactaaat ttacacacaa aacaaaatga ccgatttgaa     1020

ccaattgact caagaattgg gtgctttggg tattcacgat gtccaagaag ttgtctacaa     1080

cccatcttac gaattgttgt ttgctgaaga aaccaagcca ggtttggaag gttacgaaaa     1140

gggtactgtt accaaccaag gtgctgttgc tgtcaacacc ggtatcttca ccggtcgttc     1200

tccaaaggac aaatacattg tcttggatga caagaccaag gacactgtct ggtggacttc     1260

tgaaaaggtc aagaacgaca acaaaccaat gtcccaagac acttggaact ctttaaaggg     1320

tttagtcgct gaccaattgt ctggtaagag attattcgtt gtcgatgctt tctgtggtgc     1380

caacaaggac accagattag ctgtcagagt tgtcactgaa gttgcttggc aagctcactt     1440

cgttaccaac atgttcatca gaccatctgc tgaagaattg aaaggtttca agccagattt     1500

cgttgtcatg aacggtgcca aatgtaccaa cccaaactgg aaggaacaag gtttgaactc     1560

tgaaaacttt gttgctttca acatcactga aggtgttcaa ttgattggtg gtacctggta     1620

cggtggtgaa atgaagaagg gtatgttctc catgatgaac tacttcttgc cattgagagg     1680

tattgcttcc atgcactgtt ctgccaatgt cggtaaggac ggtgacactg ccatcttctt     1740

cggtctatcc ggtaccggta agaccacttt gtccactgac ccaaagagac aattgattgg     1800

tgatgacgaa cacggttggg atgacgaagg tgttttcaac tttgaaggtg gttgttacgc     1860

caagaccatc aacttatctg ctgaaaatga accagatatc tacggtgcca tcaagcgtga     1920

cgctctattg gaaaacgttg ttgttttgga caatggtgac gtcgattatg ctgacggttc     1980

caagactgaa aacaccagag tttcttaccc aatctaccat attcaaaaca ttgtcaagcc     2040

agtttccaag gctggtccag ctaccaaagt tatcttcttg tctgctgatg ctttcggtgt     2100

tttgcctcct gtttccaagt tgactccaga acaaaccaag tactacttct tgtctggttt     2160

caccgccaag ttggctggta ctgaaagagg tatcactgaa ccaactccaa ctttctctgc     2220

ttgtttcggt gctgcctttt tgtctttgca cccaactcaa tacgctgaag ttttggtcaa     2280

gagaatgcaa gaatctggtg ctgaagctta cttggtcaac actggttgga acggtaccgg     2340

taagagaatc tccatcaaag ataccagagg tatcatcgat gccatcttgg atggttccat     2400

tgacaaggct gaaatgggtt ctttgccaat tttcgatttc tccattccaa aggctttgcc     2460

aggtgtcaac ccagccatct tagacccaag agacacctac gctgacaaag ctcaatggga     2520

agaaaaggct caagacttgg ctggtagatt cgtcaagaac ttcgaaaaat acactggtac     2580

tgctgaaggt caagctttgg ttgctgctgg tccaaaggcc taaggcccgg gcataaagca     2640

atcttgatga ggataatgat ttttttttga atatacataa atactaccgt ttttctgcta     2700

gattttgtga agacgtaaat aagtacatat tactttttaa gccaagacaa gattaagcat     2760

taactttacc cttttctctt ctaagtttca atactagtta tcactgttta aaagttatgg     2820

cgagaacgtc ggcggttaaa atatattacc ctgaacgtgg tgaattgaag ttctaggatg     2880

gtttaaagat ttttcctttt tgggaaataa gtaaacaata tattgctgcc tttgcaaaac     2940

gcacataccc acaatatgtg actattggca aagaacgcat tatcctttga agaggtggat     3000

actgatacta agagagtctc tattccggct ccacttttag tccagagatt acttgtcttc     3060

ttacgtatca gaacaagaaa gcatttccaa agtaattgca tttgcccttg agcagtatat     3120

atatactaag aaggcgcgcc gcggccgc                                        3148


<210>  2
<211>  5037
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct TDH3p-FRDm1-TDH3t for expression in S. 
       cerevisiae

<400>  2
ggatccggcg cgccctattt tcgaggacct tgtcaccttg agcccaagag agccaagatt       60

taaattttcc tatgacttga tgcaaattcc caaagctaat aacatgcaag acacgtacgg      120

tcaagaagac atatttgacc tcttaacagg ttcagacgcg actgcctcat cagtaagacc      180

cgttgaaaag aacttacctg aaaaaaacga atatatacta gcgttgaatg ttagcgtcaa      240

caacaagaag tttaatgacg cggaggccaa ggcaaaaaga ttccttgatt acgtaaggga      300

gttagaatca ttttgaataa aaaacacgct ttttcagttc gagtttatca ttatcaatac      360

tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc      420

aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta      480

cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa      540

tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa      600

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga      660

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg      720

gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc      780

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt      840

tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg tattgattgt      900

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt      960

tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaatgggt     1020

gctgatggta tttcttctgc ttccattgtt gttactgacc cagaagctgc tgccaagaag     1080

cgtgacagaa tggccagaga attgttgtcc tccaactctg gtctatgtca agaagatgaa     1140

ccaaccatca tcaacttaaa gggtttggaa cacaccattc catacagatt ggccgttgtt     1200

ttgtgtaact ccagatccac tggtgaattc gaagccaagg ctgctgaaat cttgagaaag     1260

gctttccaca tggttgacta ctctttgaat tgtttcaacc cagaatctga attgtcccgt     1320

gtcaactctt taccagtcgg tgaaaagcac caaatgtccg aagatctaag acatgtcatg     1380

gaatgtacca tttctgtcca ccactcctct ggtatgggtt tcgacccagc tgctggtcca     1440

atcatctcca gattgagagg tgccatgaga gatcacaacg acatgtccga tatctccgtc     1500

actgaagctg aagttgaatt attctctttg gctcaatctt tcgatgtcga cttggaagaa     1560

ggtactattg ccagaaagca ctctgaagcc agattggatt tgggtggtgt caacaagggt     1620

tacactgttg actacgttgt tgaccatttg agagctgctg gtatgccaaa cgtcttgttc     1680

gaatggggtg gtgatatcag agcttctggt agaaacatca agggtaactt gtgggctgtt     1740

gccatcaagc gtccaccatc tgttgaagaa gttatccgtc gtgccaaggg taagatgtta     1800

aagatgggtg aagaagaaca agaagaaaag gacgatgact ctccatcttt gttgcacgtt     1860

gttgaattgg atgacgaagc tttgtgtacc tctggtgact acgaaaacgt cttataccat     1920

ccaaagcacg gtgttgctgg ttccattttc gactggcaac gtcgtggttt attgtctcca     1980

gaagaaggtg ctttagctca agtttccgtc aaatgttact ctgccatgta cgctgatgct     2040

ttggccactg tttgtttggt caagagagat gctgtcagaa tcagatactt gttggaaggt     2100

tggagatacg tcagatctcg tgtcaccaac tacttcgctt acaccagaca aggtgaaaga     2160

ttggctcaca tgcacgaaat tgctcaagaa accagagaat taagagaaat cagaattgct     2220

ggttctttgc catccagaat tgttatcgtc ggtggtggtt tggctggtct atccgctgcc     2280

attgaagctg cttcttgtgg tgctcaagtc attttgatgg aaaaggaagg tagaattggt     2340

ggtaactctg ccaaggctac ctctggtatc aacggttggg gtaccagaac ccaagccaag     2400

tctgatatct tggatggtgg taagtacttt gaaagagaca ctttcttgtc cggtgtcggt     2460

ggtaccactg acccagcttt ggtcaaggtc ttgtccgtca aatctggtga cgctatcggt     2520

tggttaactt ctttgggtgt cccattgtcc gttttgtctc aattgggtgg tcactctttc     2580

aagagaactc acagagctcc agacaagact gatggtactc cattaccaat tggtcacacc     2640

atcatgagaa ctttggaaga tcatatcaga aacaacttgt ctgaaagagt taccatcatg     2700

acccacgttt ctgttactga attgttgcac gaaactgaca ccactccaga tggtgcttct     2760

gaagttcgtg tcaccggtgt ccgttacaga gacttgtctg atgtcgatgg tcaaccttcc     2820

aaactattgg ctgacgctgt tgttttggcc actggtggtt tctccaacga cagagaagaa     2880

aactctttgt tgtgtaaata cgctcctcat ttggcttctt tcccaactac caacggtcca     2940

tgggctactg gtgacggtgt caaattggcc acctccgttg gtgccaagtt ggttgacatg     3000

gacaaggttc aattgcaccc aactggtttg attgacccaa aggacccagc taacaccact     3060

aagatcttgg gtccagaagc tttgagaggt tctggtggta ttttgttgaa caagcaaggt     3120

aagagattcg tcaacgaatt ggacttgaga tccgttgttt ccaaggccat taacactcaa     3180

ggtaacgaat acccaggttc tggtggttgt tactttgctt actgtgtctt aaacgaagat     3240

gctaccaact tattctgtgg tggtgctttg ggtttctacg gtaagaaatt aggtttgttc     3300

caaagagctg aaactgttga agaattggcc aaattgattg gttgtgacga aggtgaattg     3360

agagacactt tggaaaaata cgaaacctgt tccaaggcca aggttgcttg tccagtcact     3420

ggtaaggttg ttttcccatg tgttgtcggt accagaggtc catacaatgt tgctttcgtc     3480

actccatcca tccactacac catgggtggt tgtttgatct ctccagctgc tgaagtcttg     3540

caagaataca agggtttgaa tatcttggaa aaccacagac caatcagatg tttgttcggt     3600

gctggtgaag tcactggtgg tgtccacggt ggtaacagat taggtggtaa ctctctattg     3660

gaatgtgttg tctttggtaa gattgctggt gacagagctg ccactatctt gcaaaagaga     3720

gaaattgctt tgtccaagac ctcctggacc tctgttgttg tcagagaatc cagatctggt     3780

gaacaattcg gtaccggttc cagagttttg agattcaact tgccaggtgc tttacaaaga     3840

accggtttga acttgggtga attcgttgcc atcagaggtg aatgggatgg tcaacaatta     3900

gtcggttact tctctccaat cactttgcca gaagatttgg gtaccatctc tttgttggtc     3960

agagctgaca agggtacttt gaaggaatgg atctgtgctt tgcgtccagg tgactccgtt     4020

gaaatcaagg cttgtggtgg tctaagaatt gaccaagatc cagtcaagaa atgtttgttg     4080

ttcagaaaca gaccaattac cagatttgct ttggttgctg ctggtaccgg tgttgctcca     4140

atgttgcaag ttatcagagc tgctttgaag aagccatacg tcgacacttt ggaatccatc     4200

agattgatct acgctgctga agaatatgac actttaacct acagatctat cttgcaaaga     4260

tttgctgaag aattcccaga caaattcgtt tgtaacttcg tcttaaacaa ccctccagaa     4320

ggttggaccg gtggtgttgg tttcgtcaac aagaaatctt tgcaaaaggt tttgcaacca     4380

ccttcttctg aaccattgat tgttgtttgt ggtccacctg ttatgcaaag agatgtcaaa     4440

aatgaattgt tgtccatggg ttacgacaag gaattggttc acactgtcga tggtgaatct     4500

ggtaccttgt aaggcccggg cgtgaattta ctttaaatct tgcatttaaa taaattttct     4560

ttttatagct ttatgactta gtttcaattt atatactatt ttaatgacat tttcgattca     4620

ttgattgaaa gctttgtgtt ttttcttgat gcgctattgc attgttcttg tctttttcgc     4680

cacatgtaat atctgtagta gatacctgat acattgtgga tgctgagtga aattttagtt     4740

aataatggag gcgctcttaa taattttggg gatattggct ttttttttta aagtttacaa     4800

atgaattttt tccgccagga taacgattct gaagttactc ttagcgttcc tatcggtaca     4860

gccatcaaat catgcctata aatcatgcct atatttgcgt gcagtcagta tcatctacat     4920

gaaaaaaact cccgcaattt cttatagaat acgttgaaaa ttaaatgtac gcgccaagat     4980

aagataacat atatctagat gcagtaatat acacagattc cggccggccg cggccgc        5037


<210>  3
<211>  2950
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct TDH1p-FUMR-TDH1t for expression in S. 
       cerevisiae

<400>  3
ggatcccttc ccttttacag tgcttcggaa aagcacagcg ttgtccaagg gaacaatttt       60

tcttcaagtt aatgcataag aaatatcttt ttttatgttt agctaagtaa aagcagcttg      120

gagtaaaaaa aaaaatgagt aaatttctcg atggattagt ttctcacagg taacataaca      180

aaaaccaaga aaagcccgct tctgaaaact acagttgact tgtatgctaa agggccagac      240

taatgggagg agaaaaagaa acgaatgtat atgctcattt acactctata tcaccatatg      300

gaggataagt tgggctgagc ttctgatcca atttattcta tccattagtt gctgatatgt      360

cccaccagcc aacacttgat agtatctact cgccattcac ttccagcagc gccagtaggg      420

ttgttgagct tagtaaaaat gtgcgcacca caagcctaca tgactccacg tcacatgaaa      480

ccacaccgtg gggccttgtt gcgctaggaa taggatatgc gacgaagacg cttctgctta      540

gtaaccacac cacattttca gggggtcgat ctgcttgctt cctttactgt cacgagcggc      600

ccataatcgc gctttttttt taaaaggcgc gagacagcaa acaggaagct cgggtttcaa      660

ccttcggagt ggtcgcagat ctggagactg gatctttaca atacagtaag gcaagccacc      720

atctgcttct taggtgcatg cgacggtatc cacgtgcaga acaacatagt ctgaagaagg      780

gggggaggag catgttcatt ctctgtagca gtaagagctt ggtgataatg accaaaactg      840

gagtctcgaa atcatataaa tagacaatat attttcacac aatgagattt gtagtacagt      900

tctattctct ctcttgcata aataagaaat tcatcaagaa cttggtttga tatttcacca      960

acacacacaa aaaacagtac ttcactaaat ttacacacaa aacaaaatgt cctctgcttc     1020

tgctgctttg caaaaattca gagctgaaag agataccttc ggtgacttgc aagttccagc     1080

tgaccgttac tggggtgctc aaactcaaag atctttgcaa aactttgaca ttggtggtcc     1140

aactgaaaga atgccagaac cattaatcag agctttcggt gttttgaaga aggctgctgc     1200

caccgtcaac atgacctacg gtttggaccc aaaggttggt gaagccatcc aaaaggctgc     1260

tgacgaagtt atcgatggtt ctttgattga ccatttccca ttggttgtct ggcaaaccgg     1320

ttctggtact caaaccaaga tgaacgtcaa tgaagtcatc tccaacagag ccattgaatt     1380

gttgggtggt gaattaggtt ccaaggctcc agtccaccca aacgatcatg tcaacatgtc     1440

tcaatcttcc aacgacactt tcccaactgc catgcacgtt gctgccgttg ttgaaattca     1500

cggtagattg attccagctt tgaccacttt gagagatgct ttgcaagcca aatctgctga     1560

attcgaacac atcatcaaga ttggtagaac ccacttgcaa gatgctaccc cattgacttt     1620

aggtcaagaa ttctccggtt acactcaaca attgacctac ggtattgctc gtgttcaagg     1680

tactttggaa agattataca acttggctca aggtggtact gctgtcggta ctggtttgaa     1740

caccagaaag ggtttcgatg ccaaggttgc tgaagccatt gcttccatca ctggtttacc     1800

attcaagacc gctccaaaca aattcgaagc tttggctgct cacgacgctt tggttgaagc     1860

tcacggtgct ttgaacaccg ttgcttgttc tttgatgaag attgccaacg atatccgtta     1920

cttgggttct ggtccaagat gtggtttagg tgaattgtct ctaccagaaa acgaaccagg     1980

ttcttccatc atgccaggta aggtcaaccc aactcaatgt gaagctatga ccatggtttg     2040

tgctcaagtc atgggtaaca acactgccat ctctgttgct ggttccaacg gtcaattcga     2100

attgaatgtc tttaaaccag tcatgatcaa gaacttgatc caatccatca gattaatctc     2160

tgacgcttcc atctctttca ccaagaactg tgttgtcggt attgaagcta acgaaaagaa     2220

gatctcctcc atcatgaacg aatctttgat gttggtcact gctttgaacc ctcacattgg     2280

ttacgacaag gctgccaagt gtgccaagaa ggctcacaag gaaggtacca ctttgaaaga     2340

agctgctcta tctttgggtt acttgacctc tgaagaattc gaccaatggg ttagacctga     2400

ggacatgatt tctgccaagg attaaggccc gggcataaag caatcttgat gaggataatg     2460

attttttttt gaatatacat aaatactacc gtttttctgc tagattttgt gaagacgtaa     2520

ataagtacat attacttttt aagccaagac aagattaagc attaacttta cccttttctc     2580

ttctaagttt caatactagt tatcactgtt taaaagttat ggcgagaacg tcggcggtta     2640

aaatatatta ccctgaacgt ggtgaattga agttctagga tggtttaaag atttttcctt     2700

tttgggaaat aagtaaacaa tatattgctg cctttgcaaa acgcacatac ccacaatatg     2760

tgactattgg caaagaacgc attatccttt gaagaggtgg atactgatac taagagagtc     2820

tctattccgg ctccactttt agtccagaga ttacttgtct tcttacgtat cagaacaaga     2880

aagcatttcc aaagtaattg catttgccct tgagcagtat atatatacta agaaggcgcg     2940

ccgcggccgc                                                            2950


<210>  4
<211>  2637
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct TDH3p-delta12N MDH2-TDH3t for expression in 
       S. cerevisiae

<400>  4
ggatccggcg cgccctattt tcgaggacct tgtcaccttg agcccaagag agccaagatt       60

taaattttcc tatgacttga tgcaaattcc caaagctaat aacatgcaag acacgtacgg      120

tcaagaagac atatttgacc tcttaacagg ttcagacgcg actgcctcat cagtaagacc      180

cgttgaaaag aacttacctg aaaaaaacga atatatacta gcgttgaatg ttagcgtcaa      240

caacaagaag tttaatgacg cggaggccaa ggcaaaaaga ttccttgatt acgtaaggga      300

gttagaatca ttttgaataa aaaacacgct ttttcagttc gagtttatca ttatcaatac      360

tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc      420

aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta      480

cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa      540

tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa      600

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga      660

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg      720

gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc      780

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt      840

tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg tattgattgt      900

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt      960

tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaatgttg     1020

aagattgcca tcttgggtgc tgctggtggt atcggtcaat ctttgtcttt gttgttgaag     1080

gctcaattgc aataccaatt gaaggaatcc aacagatctg ttacccacat tcatttggct     1140

ttgtacgatg tcaaccaaga agctatcaac ggtgtcactg ctgacttgtc tcacatcgat     1200

accccaatct ctgtttcctc tcactctcca gctggtggta ttgaaaactg tttgcacaac     1260

gcttccattg ttgtcattcc agccggtgtt ccaagaaagc caggtatgac ccgtgacgat     1320

ttgttcaacg tcaatgccgg tatcatctct caattaggtg attccattgc tgaatgttgt     1380

gacttgtcca aggttttcgt cttggttatc tccaacccag tcaactcttt ggttcctgtt     1440

atggtttcca acatcttgaa gaaccaccca caatccagaa actctggtat tgaaagaaga     1500

atcatgggtg tcaccaaatt ggacattgtc agagcttcca ctttcttgag agaaatcaac     1560

attgaatctg gtttgactcc aagagtcaac tccatgccag atgttccagt tatcggtggt     1620

cactctggtg aaactatcat cccattattc tctcaatcta acttcttgtc cagattgaat     1680

gaagatcaat tgaaatactt gattcaccgt gtccaatacg gtggtgacga agttgtcaag     1740

gccaagaacg gtaagggttc tgctactcta tccatggctc atgccggtta caagtgtgtt     1800

gtccaattcg tttctctatt attaggtaac attgaacaaa tccacggtac ctactacgtt     1860

ccattgaaag atgctaacaa cttcccaatt gctccaggtg ctgaccaatt attgccatta     1920

gtcgacggtg ctgactactt tgccatccca ttgaccatca ctaccaaggg tgtttcttac     1980

gttgactacg atatcgtcaa cagaatgaac gacatggaaa gaaaccaaat gttgcctatc     2040

tgtgtttctc aattgaagaa gaacattgac aagggtttgg aattcgttgc ttccagatct     2100

gcttccagtt aaggcccggg cgtgaattta ctttaaatct tgcatttaaa taaattttct     2160

ttttatagct ttatgactta gtttcaattt atatactatt ttaatgacat tttcgattca     2220

ttgattgaaa gctttgtgtt ttttcttgat gcgctattgc attgttcttg tctttttcgc     2280

cacatgtaat atctgtagta gatacctgat acattgtgga tgctgagtga aattttagtt     2340

aataatggag gcgctcttaa taattttggg gatattggct ttttttttta aagtttacaa     2400

atgaattttt tccgccagga taacgattct gaagttactc ttagcgttcc tatcggtaca     2460

gccatcaaat catgcctata aatcatgcct atatttgcgt gcagtcagta tcatctacat     2520

gaaaaaaact cccgcaattt cttatagaat acgttgaaaa ttaaatgtac gcgccaagat     2580

aagataacat atatctagat gcagtaatat acacagattc cggccggccg cggccgc        2637


<210>  5
<211>  2240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct Eno1p-SpMAE1-ENOt for expression in S. 
       cerevisiae

<400>  5
ggatccggcg cgccccgcgg aaccgccaga tattcattac ttgacgcaaa agcgtttgaa       60

ataatgacga aaaagaagga agaaaaaaaa agaaaaatac cgcttctagg cgggttatct      120

actgatccga gcttccacta ggatagcacc caaacacctg catatttgga cgacctttac      180

ttacaccacc aaaaaccact ttcgcctctc ccgcccctga taacgtccac taattgagcg      240

attacctgag cggtcctctt ttgtttgcag catgagactt gcatactgca aatcgtaagt      300

agcaacgtct caaggtcaaa actgtatgga aaccttgtca cctcacttaa ttctagctag      360

cctaccctgc aagtcaagag gtctccgtga ttcctagcca cctcaaggta tgcctctccc      420

cggaaactgt ggccttttct ggcacacatg atctccacga tttcaacata taaatagctt      480

ttgataatgg caatattaat caaatttatt ttacttcttt cttgtaacat ctctcttgta      540

atcccttatt ccttctagct atttttcata aaaaaccaag caactgctta tcaacacaca      600

aacactaaaa caaaatgggt gaattgaagg aaatcttgaa gcaacgttac catgaattgt      660

tggactggaa cgtcaaggct ccacacgttc cattgtctca aagattgaag catttcacct      720

ggtcctggtt tgcttgtacc atggccactg gtggtgtcgg tttgatcatt ggttctttcc      780

cattcagatt ctacggtttg aacaccattg gtaagattgt ctacatctta caaatcttct      840

tattctcttt gtttggttct tgtatgttgt tcagattcat caaataccca tctaccatca      900

aggactcctg gaaccaccac ttggaaaaat tattcattgc tacctgtttg ctatccatct      960

ccactttcat tgacatgttg gccatctacg cttacccaga cactggtgaa tggatggtct     1020

gggttatcag aatcttatac tacatctacg ttgctgtctc tttcatctac tgtgtcatgg     1080

ctttcttcac cattttcaac aaccacgttt acaccattga aactgcttct ccagcttgga     1140

tcttaccaat tttcccacca atgatctgtg gtgtcattgc tggtgctgtc aactccactc     1200

aaccagctca ccaattgaag aacatggtta tcttcggtat cttattccaa ggtttgggtt     1260

tctgggttta cttgttgttg tttgctgtca acgttttgag attcttcacc gttggtttgg     1320

ccaagcctca agacagacca ggtatgttca tgtttgttgg tccaccagct ttctccggtt     1380

tggctttgat caacattgcc cgtggtgcta tgggttccag accatacatt ttcgtcggtg     1440

ccaattcttc tgaatacttg ggtttcgttt ccactttcat ggccattttc atctggggtt     1500

tggctgcttg gtgttactgt ttggccatgg tttctttctt ggctggtttc ttcaccagag     1560

ctccattgaa atttgcttgt ggttggtttg ctttcatctt cccaaacgtc ggtttcgtta     1620

actgtaccat tgaaattggt aagatgattg actccaaggc cttccaaatg ttcggtcaca     1680

tcatcggtgt catcctatgt atccaatgga tcttgttgat gtacttgatg gtcagagctt     1740

tcttggtcaa cgatttgtgt tacccaggta aggatgaaga tgctcaccca cctccaaagc     1800

caaacactgg tgttttgaac ccaactttcc caccagaaaa ggctccagct tctttggaaa     1860

aggttgacac ccacgttact tccactggtg gtgaatctga tcctccatct tctgaacacg     1920

aaagcgttta agagcttttg attaagcctt ctagtccaaa aaacacgttt ttttgtcatt     1980

tatttcattt tcttagaata gtttagttta ttcattttat agtcacgaat gttttatgat     2040

tctatatagg gttgcaaaca agcatttttc attttatgtt aaaacaattt caggtttacc     2100

ttttattctg cttgtggtga cgcgggtatc cgcccgctct tttggtcacc catgtattta     2160

attgcataaa taattcttaa aagtggagct agtctatttc tatttacata cctctcattt     2220

ctcatttcct ccgcggccgc                                                 2240


<210>  6
<211>  538
<212>  PRT
<213>  Mannheimia succiniciproducens

<400>  6

Met Thr Asp Leu Asn Gln Leu Thr Gln Glu Leu Gly Ala Leu Gly Ile 
1               5                   10                  15      


His Asp Val Gln Glu Val Val Tyr Asn Pro Ser Tyr Glu Leu Leu Phe 
            20                  25                  30          


Ala Glu Glu Thr Lys Pro Gly Leu Glu Gly Tyr Glu Lys Gly Thr Val 
        35                  40                  45              


Thr Asn Gln Gly Ala Val Ala Val Asn Thr Gly Ile Phe Thr Gly Arg 
    50                  55                  60                  


Ser Pro Lys Asp Lys Tyr Ile Val Leu Asp Asp Lys Thr Lys Asp Thr 
65                  70                  75                  80  


Val Trp Trp Thr Ser Glu Lys Val Lys Asn Asp Asn Lys Pro Met Ser 
                85                  90                  95      


Gln Asp Thr Trp Asn Ser Leu Lys Gly Leu Val Ala Asp Gln Leu Ser 
            100                 105                 110         


Gly Lys Arg Leu Phe Val Val Asp Ala Phe Cys Gly Ala Asn Lys Asp 
        115                 120                 125             


Thr Arg Leu Ala Val Arg Val Val Thr Glu Val Ala Trp Gln Ala His 
    130                 135                 140                 


Phe Val Thr Asn Met Phe Ile Arg Pro Ser Ala Glu Glu Leu Lys Gly 
145                 150                 155                 160 


Phe Lys Pro Asp Phe Val Val Met Asn Gly Ala Lys Cys Thr Asn Pro 
                165                 170                 175     


Asn Trp Lys Glu Gln Gly Leu Asn Ser Glu Asn Phe Val Ala Phe Asn 
            180                 185                 190         


Ile Thr Glu Gly Val Gln Leu Ile Gly Gly Thr Trp Tyr Gly Gly Glu 
        195                 200                 205             


Met Lys Lys Gly Met Phe Ser Met Met Asn Tyr Phe Leu Pro Leu Arg 
    210                 215                 220                 


Gly Ile Ala Ser Met His Cys Ser Ala Asn Val Gly Lys Asp Gly Asp 
225                 230                 235                 240 


Thr Ala Ile Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser 
                245                 250                 255     


Thr Asp Pro Lys Arg Gln Leu Ile Gly Asp Asp Glu His Gly Trp Asp 
            260                 265                 270         


Asp Glu Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys Thr Ile 
        275                 280                 285             


Asn Leu Ser Ala Glu Asn Glu Pro Asp Ile Tyr Gly Ala Ile Lys Arg 
    290                 295                 300                 


Asp Ala Leu Leu Glu Asn Val Val Val Leu Asp Asn Gly Asp Val Asp 
305                 310                 315                 320 


Tyr Ala Asp Gly Ser Lys Thr Glu Asn Thr Arg Val Ser Tyr Pro Ile 
                325                 330                 335     


Tyr His Ile Gln Asn Ile Val Lys Pro Val Ser Lys Ala Gly Pro Ala 
            340                 345                 350         


Thr Lys Val Ile Phe Leu Ser Ala Asp Ala Phe Gly Val Leu Pro Pro 
        355                 360                 365             


Val Ser Lys Leu Thr Pro Glu Gln Thr Lys Tyr Tyr Phe Leu Ser Gly 
    370                 375                 380                 


Phe Thr Ala Lys Leu Ala Gly Thr Glu Arg Gly Ile Thr Glu Pro Thr 
385                 390                 395                 400 


Pro Thr Phe Ser Ala Cys Phe Gly Ala Ala Phe Leu Ser Leu His Pro 
                405                 410                 415     


Thr Gln Tyr Ala Glu Val Leu Val Lys Arg Met Gln Glu Ser Gly Ala 
            420                 425                 430         


Glu Ala Tyr Leu Val Asn Thr Gly Trp Asn Gly Thr Gly Lys Arg Ile 
        435                 440                 445             


Ser Ile Lys Asp Thr Arg Gly Ile Ile Asp Ala Ile Leu Asp Gly Ser 
    450                 455                 460                 


Ile Asp Lys Ala Glu Met Gly Ser Leu Pro Ile Phe Asp Phe Ser Ile 
465                 470                 475                 480 


Pro Lys Ala Leu Pro Gly Val Asn Pro Ala Ile Leu Asp Pro Arg Asp 
                485                 490                 495     


Thr Tyr Ala Asp Lys Ala Gln Trp Glu Glu Lys Ala Gln Asp Leu Ala 
            500                 505                 510         


Gly Arg Phe Val Lys Asn Phe Glu Lys Tyr Thr Gly Thr Ala Glu Gly 
        515                 520                 525             


Gln Ala Leu Val Ala Ala Gly Pro Lys Ala 
    530                 535             


<210>  7
<211>  1164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mitochondrial Trypanosoma brucei fumarate reductase (FRDm1) amino
       acid sequence, lacking the 68 N-terminal residues

<400>  7

Met Ala Asp Gly Ile Ser Ser Ala Ser Ile Val Val Thr Asp Pro Glu 
1               5                   10                  15      


Ala Ala Ala Lys Lys Arg Asp Arg Met Ala Arg Glu Leu Leu Ser Ser 
            20                  25                  30          


Asn Ser Gly Leu Cys Gln Glu Asp Glu Pro Thr Ile Ile Asn Leu Lys 
        35                  40                  45              


Gly Leu Glu His Thr Ile Pro Tyr Arg Leu Ala Val Val Leu Cys Asn 
    50                  55                  60                  


Ser Arg Ser Thr Gly Glu Phe Glu Ala Lys Ala Ala Glu Ile Leu Arg 
65                  70                  75                  80  


Lys Ala Phe His Met Val Asp Tyr Ser Leu Asn Cys Phe Asn Pro Glu 
                85                  90                  95      


Ser Glu Leu Ser Arg Val Asn Ser Leu Pro Val Gly Glu Lys His Gln 
            100                 105                 110         


Met Ser Glu Asp Leu Arg His Val Met Glu Cys Thr Ile Ser Val His 
        115                 120                 125             


His Ser Ser Gly Met Gly Phe Asp Pro Ala Ala Gly Pro Ile Ile Ser 
    130                 135                 140                 


Arg Leu Arg Gly Ala Met Arg Asp His Asn Asp Met Ser Asp Ile Ser 
145                 150                 155                 160 


Val Thr Glu Ala Glu Val Glu Leu Phe Ser Leu Ala Gln Ser Phe Asp 
                165                 170                 175     


Val Asp Leu Glu Glu Gly Thr Ile Ala Arg Lys His Ser Glu Ala Arg 
            180                 185                 190         


Leu Asp Leu Gly Gly Val Asn Lys Gly Tyr Thr Val Asp Tyr Val Val 
        195                 200                 205             


Asp His Leu Arg Ala Ala Gly Met Pro Asn Val Leu Phe Glu Trp Gly 
    210                 215                 220                 


Gly Asp Ile Arg Ala Ser Gly Arg Asn Ile Lys Gly Asn Leu Trp Ala 
225                 230                 235                 240 


Val Ala Ile Lys Arg Pro Pro Ser Val Glu Glu Val Ile Arg Arg Ala 
                245                 250                 255     


Lys Gly Lys Met Leu Lys Met Gly Glu Glu Glu Gln Glu Glu Lys Asp 
            260                 265                 270         


Asp Asp Ser Pro Ser Leu Leu His Val Val Glu Leu Asp Asp Glu Ala 
        275                 280                 285             


Leu Cys Thr Ser Gly Asp Tyr Glu Asn Val Leu Tyr His Pro Lys His 
    290                 295                 300                 


Gly Val Ala Gly Ser Ile Phe Asp Trp Gln Arg Arg Gly Leu Leu Ser 
305                 310                 315                 320 


Pro Glu Glu Gly Ala Leu Ala Gln Val Ser Val Lys Cys Tyr Ser Ala 
                325                 330                 335     


Met Tyr Ala Asp Ala Leu Ala Thr Val Cys Leu Val Lys Arg Asp Ala 
            340                 345                 350         


Val Arg Ile Arg Tyr Leu Leu Glu Gly Trp Arg Tyr Val Arg Ser Arg 
        355                 360                 365             


Val Thr Asn Tyr Phe Ala Tyr Thr Arg Gln Gly Glu Arg Leu Ala His 
    370                 375                 380                 


Met His Glu Ile Ala Gln Glu Thr Arg Glu Leu Arg Glu Ile Arg Ile 
385                 390                 395                 400 


Ala Gly Ser Leu Pro Ser Arg Ile Val Ile Val Gly Gly Gly Leu Ala 
                405                 410                 415     


Gly Leu Ser Ala Ala Ile Glu Ala Ala Ser Cys Gly Ala Gln Val Ile 
            420                 425                 430         


Leu Met Glu Lys Glu Gly Arg Ile Gly Gly Asn Ser Ala Lys Ala Thr 
        435                 440                 445             


Ser Gly Ile Asn Gly Trp Gly Thr Arg Thr Gln Ala Lys Ser Asp Ile 
    450                 455                 460                 


Leu Asp Gly Gly Lys Tyr Phe Glu Arg Asp Thr Phe Leu Ser Gly Val 
465                 470                 475                 480 


Gly Gly Thr Thr Asp Pro Ala Leu Val Lys Val Leu Ser Val Lys Ser 
                485                 490                 495     


Gly Asp Ala Ile Gly Trp Leu Thr Ser Leu Gly Val Pro Leu Ser Val 
            500                 505                 510         


Leu Ser Gln Leu Gly Gly His Ser Phe Lys Arg Thr His Arg Ala Pro 
        515                 520                 525             


Asp Lys Thr Asp Gly Thr Pro Leu Pro Ile Gly His Thr Ile Met Arg 
    530                 535                 540                 


Thr Leu Glu Asp His Ile Arg Asn Asn Leu Ser Glu Arg Val Thr Ile 
545                 550                 555                 560 


Met Thr His Val Ser Val Thr Glu Leu Leu His Glu Thr Asp Thr Thr 
                565                 570                 575     


Pro Asp Gly Ala Ser Glu Val Arg Val Thr Gly Val Arg Tyr Arg Asp 
            580                 585                 590         


Leu Ser Asp Val Asp Gly Gln Pro Ser Lys Leu Leu Ala Asp Ala Val 
        595                 600                 605             


Val Leu Ala Thr Gly Gly Phe Ser Asn Asp Arg Glu Glu Asn Ser Leu 
    610                 615                 620                 


Leu Cys Lys Tyr Ala Pro His Leu Ala Ser Phe Pro Thr Thr Asn Gly 
625                 630                 635                 640 


Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala Thr Ser Val Gly Ala 
                645                 650                 655     


Lys Leu Val Asp Met Asp Lys Val Gln Leu His Pro Thr Gly Leu Ile 
            660                 665                 670         


Asp Pro Lys Asp Pro Ala Asn Thr Thr Lys Ile Leu Gly Pro Glu Ala 
        675                 680                 685             


Leu Arg Gly Ser Gly Gly Ile Leu Leu Asn Lys Gln Gly Lys Arg Phe 
    690                 695                 700                 


Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser Lys Ala Ile Asn Thr 
705                 710                 715                 720 


Gln Gly Asn Glu Tyr Pro Gly Ser Gly Gly Cys Tyr Phe Ala Tyr Cys 
                725                 730                 735     


Val Leu Asn Glu Asp Ala Thr Asn Leu Phe Cys Gly Gly Ala Leu Gly 
            740                 745                 750         


Phe Tyr Gly Lys Lys Leu Gly Leu Phe Gln Arg Ala Glu Thr Val Glu 
        755                 760                 765             


Glu Leu Ala Lys Leu Ile Gly Cys Asp Glu Gly Glu Leu Arg Asp Thr 
    770                 775                 780                 


Leu Glu Lys Tyr Glu Thr Cys Ser Lys Ala Lys Val Ala Cys Pro Val 
785                 790                 795                 800 


Thr Gly Lys Val Val Phe Pro Cys Val Val Gly Thr Arg Gly Pro Tyr 
                805                 810                 815     


Asn Val Ala Phe Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys 
            820                 825                 830         


Leu Ile Ser Pro Ala Ala Glu Val Leu Gln Glu Tyr Lys Gly Leu Asn 
        835                 840                 845             


Ile Leu Glu Asn His Arg Pro Ile Arg Cys Leu Phe Gly Ala Gly Glu 
    850                 855                 860                 


Val Thr Gly Gly Val His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu 
865                 870                 875                 880 


Leu Glu Cys Val Val Phe Gly Lys Ile Ala Gly Asp Arg Ala Ala Thr 
                885                 890                 895     


Ile Leu Gln Lys Arg Glu Ile Ala Leu Ser Lys Thr Ser Trp Thr Ser 
            900                 905                 910         


Val Val Val Arg Glu Ser Arg Ser Gly Glu Gln Phe Gly Thr Gly Ser 
        915                 920                 925             


Arg Val Leu Arg Phe Asn Leu Pro Gly Ala Leu Gln Arg Thr Gly Leu 
    930                 935                 940                 


Asn Leu Gly Glu Phe Val Ala Ile Arg Gly Glu Trp Asp Gly Gln Gln 
945                 950                 955                 960 


Leu Val Gly Tyr Phe Ser Pro Ile Thr Leu Pro Glu Asp Leu Gly Thr 
                965                 970                 975     


Ile Ser Leu Leu Val Arg Ala Asp Lys Gly Thr Leu Lys Glu Trp Ile 
            980                 985                 990         


Cys Ala Leu Arg Pro Gly Asp Ser  Val Glu Ile Lys Ala  Cys Gly Gly 
        995                 1000                 1005             


Leu Arg  Ile Asp Gln Asp Pro  Val Lys Lys Cys Leu  Leu Phe Arg 
    1010                 1015                 1020             


Asn Arg  Pro Ile Thr Arg Phe  Ala Leu Val Ala Ala  Gly Thr Gly 
    1025                 1030                 1035             


Val Ala  Pro Met Leu Gln Val  Ile Arg Ala Ala Leu  Lys Lys Pro 
    1040                 1045                 1050             


Tyr Val  Asp Thr Leu Glu Ser  Ile Arg Leu Ile Tyr  Ala Ala Glu 
    1055                 1060                 1065             


Glu Tyr  Asp Thr Leu Thr Tyr  Arg Ser Ile Leu Gln  Arg Phe Ala 
    1070                 1075                 1080             


Glu Glu  Phe Pro Asp Lys Phe  Val Cys Asn Phe Val  Leu Asn Asn 
    1085                 1090                 1095             


Pro Pro  Glu Gly Trp Thr Gly  Gly Val Gly Phe Val  Asn Lys Lys 
    1100                 1105                 1110             


Ser Leu  Gln Lys Val Leu Gln  Pro Pro Ser Ser Glu  Pro Leu Ile 
    1115                 1120                 1125             


Val Val  Cys Gly Pro Pro Val  Met Gln Arg Asp Val  Lys Asn Glu 
    1130                 1135                 1140             


Leu Leu  Ser Met Gly Tyr Asp  Lys Glu Leu Val His  Thr Val Asp 
    1145                 1150                 1155             


Gly Glu  Ser Gly Thr Leu 
    1160                 


<210>  8
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rhizopus oryzae fumarase amino acid sequence, lacking first 23 
       amino acids,

<400>  8

Met Ser Ser Ala Ser Ala Ala Leu Gln Lys Phe Arg Ala Glu Arg Asp 
1               5                   10                  15      


Thr Phe Gly Asp Leu Gln Val Pro Ala Asp Arg Tyr Trp Gly Ala Gln 
            20                  25                  30          


Thr Gln Arg Ser Leu Gln Asn Phe Asp Ile Gly Gly Pro Thr Glu Arg 
        35                  40                  45              


Met Pro Glu Pro Leu Ile Arg Ala Phe Gly Val Leu Lys Lys Ala Ala 
    50                  55                  60                  


Ala Thr Val Asn Met Thr Tyr Gly Leu Asp Pro Lys Val Gly Glu Ala 
65                  70                  75                  80  


Ile Gln Lys Ala Ala Asp Glu Val Ile Asp Gly Ser Leu Ile Asp His 
                85                  90                  95      


Phe Pro Leu Val Val Trp Gln Thr Gly Ser Gly Thr Gln Thr Lys Met 
            100                 105                 110         


Asn Val Asn Glu Val Ile Ser Asn Arg Ala Ile Glu Leu Leu Gly Gly 
        115                 120                 125             


Glu Leu Gly Ser Lys Ala Pro Val His Pro Asn Asp His Val Asn Met 
    130                 135                 140                 


Ser Gln Ser Ser Asn Asp Thr Phe Pro Thr Ala Met His Val Ala Ala 
145                 150                 155                 160 


Val Val Glu Ile His Gly Arg Leu Ile Pro Ala Leu Thr Thr Leu Arg 
                165                 170                 175     


Asp Ala Leu Gln Ala Lys Ser Ala Glu Phe Glu His Ile Ile Lys Ile 
            180                 185                 190         


Gly Arg Thr His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu 
        195                 200                 205             


Phe Ser Gly Tyr Thr Gln Gln Leu Thr Tyr Gly Ile Ala Arg Val Gln 
    210                 215                 220                 


Gly Thr Leu Glu Arg Leu Tyr Asn Leu Ala Gln Gly Gly Thr Ala Val 
225                 230                 235                 240 


Gly Thr Gly Leu Asn Thr Arg Lys Gly Phe Asp Ala Lys Val Ala Glu 
                245                 250                 255     


Ala Ile Ala Ser Ile Thr Gly Leu Pro Phe Lys Thr Ala Pro Asn Lys 
            260                 265                 270         


Phe Glu Ala Leu Ala Ala His Asp Ala Leu Val Glu Ala His Gly Ala 
        275                 280                 285             


Leu Asn Thr Val Ala Cys Ser Leu Met Lys Ile Ala Asn Asp Ile Arg 
    290                 295                 300                 


Tyr Leu Gly Ser Gly Pro Arg Cys Gly Leu Gly Glu Leu Ser Leu Pro 
305                 310                 315                 320 


Glu Asn Glu Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr 
                325                 330                 335     


Gln Cys Glu Ala Met Thr Met Val Cys Ala Gln Val Met Gly Asn Asn 
            340                 345                 350         


Thr Ala Ile Ser Val Ala Gly Ser Asn Gly Gln Phe Glu Leu Asn Val 
        355                 360                 365             


Phe Lys Pro Val Met Ile Lys Asn Leu Ile Gln Ser Ile Arg Leu Ile 
    370                 375                 380                 


Ser Asp Ala Ser Ile Ser Phe Thr Lys Asn Cys Val Val Gly Ile Glu 
385                 390                 395                 400 


Ala Asn Glu Lys Lys Ile Ser Ser Ile Met Asn Glu Ser Leu Met Leu 
                405                 410                 415     


Val Thr Ala Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ala Lys Cys 
            420                 425                 430         


Ala Lys Lys Ala His Lys Glu Gly Thr Thr Leu Lys Glu Ala Ala Leu 
        435                 440                 445             


Ser Leu Gly Tyr Leu Thr Ser Glu Glu Phe Asp Gln Trp Val Arg Pro 
    450                 455                 460                 


Glu Asp Met Ile Ser Ala Lys Asp 
465                 470         


<210>  9
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Saccharomyces cerevisiae malate dehydrogenase lacking first 12 
       amino acids

<400>  9

Met Leu Lys Ile Ala Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Ala Gln Leu Gln Tyr Gln Leu Lys Glu Ser 
            20                  25                  30          


Asn Arg Ser Val Thr His Ile His Leu Ala Leu Tyr Asp Val Asn Gln 
        35                  40                  45              


Glu Ala Ile Asn Gly Val Thr Ala Asp Leu Ser His Ile Asp Thr Pro 
    50                  55                  60                  


Ile Ser Val Ser Ser His Ser Pro Ala Gly Gly Ile Glu Asn Cys Leu 
65                  70                  75                  80  


His Asn Ala Ser Ile Val Val Ile Pro Ala Gly Val Pro Arg Lys Pro 
                85                  90                  95      


Gly Met Thr Arg Asp Asp Leu Phe Asn Val Asn Ala Gly Ile Ile Ser 
            100                 105                 110         


Gln Leu Gly Asp Ser Ile Ala Glu Cys Cys Asp Leu Ser Lys Val Phe 
        115                 120                 125             


Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Val Met Val 
    130                 135                 140                 


Ser Asn Ile Leu Lys Asn His Pro Gln Ser Arg Asn Ser Gly Ile Glu 
145                 150                 155                 160 


Arg Arg Ile Met Gly Val Thr Lys Leu Asp Ile Val Arg Ala Ser Thr 
                165                 170                 175     


Phe Leu Arg Glu Ile Asn Ile Glu Ser Gly Leu Thr Pro Arg Val Asn 
            180                 185                 190         


Ser Met Pro Asp Val Pro Val Ile Gly Gly His Ser Gly Glu Thr Ile 
        195                 200                 205             


Ile Pro Leu Phe Ser Gln Ser Asn Phe Leu Ser Arg Leu Asn Glu Asp 
    210                 215                 220                 


Gln Leu Lys Tyr Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val 
225                 230                 235                 240 


Val Lys Ala Lys Asn Gly Lys Gly Ser Ala Thr Leu Ser Met Ala His 
                245                 250                 255     


Ala Gly Tyr Lys Cys Val Val Gln Phe Val Ser Leu Leu Leu Gly Asn 
            260                 265                 270         


Ile Glu Gln Ile His Gly Thr Tyr Tyr Val Pro Leu Lys Asp Ala Asn 
        275                 280                 285             


Asn Phe Pro Ile Ala Pro Gly Ala Asp Gln Leu Leu Pro Leu Val Asp 
    290                 295                 300                 


Gly Ala Asp Tyr Phe Ala Ile Pro Leu Thr Ile Thr Thr Lys Gly Val 
305                 310                 315                 320 


Ser Tyr Val Asp Tyr Asp Ile Val Asn Arg Met Asn Asp Met Glu Arg 
                325                 330                 335     


Asn Gln Met Leu Pro Ile Cys Val Ser Gln Leu Lys Lys Asn Ile Asp 
            340                 345                 350         


Lys Gly Leu Glu Phe Val Ala Ser Arg Ser Ala Ser Ser 
        355                 360                 365 


<210>  10
<211>  438
<212>  PRT
<213>  Schizosaccharomyces pombe

<400>  10

Met Gly Glu Leu Lys Glu Ile Leu Lys Gln Arg Tyr His Glu Leu Leu 
1               5                   10                  15      


Asp Trp Asn Val Lys Ala Pro His Val Pro Leu Ser Gln Arg Leu Lys 
            20                  25                  30          


His Phe Thr Trp Ser Trp Phe Ala Cys Thr Met Ala Thr Gly Gly Val 
        35                  40                  45              


Gly Leu Ile Ile Gly Ser Phe Pro Phe Arg Phe Tyr Gly Leu Asn Thr 
    50                  55                  60                  


Ile Gly Lys Ile Val Tyr Ile Leu Gln Ile Phe Leu Phe Ser Leu Phe 
65                  70                  75                  80  


Gly Ser Cys Met Leu Phe Arg Phe Ile Lys Tyr Pro Ser Thr Ile Lys 
                85                  90                  95      


Asp Ser Trp Asn His His Leu Glu Lys Leu Phe Ile Ala Thr Cys Leu 
            100                 105                 110         


Leu Ser Ile Ser Thr Phe Ile Asp Met Leu Ala Ile Tyr Ala Tyr Pro 
        115                 120                 125             


Asp Thr Gly Glu Trp Met Val Trp Val Ile Arg Ile Leu Tyr Tyr Ile 
    130                 135                 140                 


Tyr Val Ala Val Ser Phe Ile Tyr Cys Val Met Ala Phe Phe Thr Ile 
145                 150                 155                 160 


Phe Asn Asn His Val Tyr Thr Ile Glu Thr Ala Ser Pro Ala Trp Ile 
                165                 170                 175     


Leu Pro Ile Phe Pro Pro Met Ile Cys Gly Val Ile Ala Gly Ala Val 
            180                 185                 190         


Asn Ser Thr Gln Pro Ala His Gln Leu Lys Asn Met Val Ile Phe Gly 
        195                 200                 205             


Ile Leu Phe Gln Gly Leu Gly Phe Trp Val Tyr Leu Leu Leu Phe Ala 
    210                 215                 220                 


Val Asn Val Leu Arg Phe Phe Thr Val Gly Leu Ala Lys Pro Gln Asp 
225                 230                 235                 240 


Arg Pro Gly Met Phe Met Phe Val Gly Pro Pro Ala Phe Ser Gly Leu 
                245                 250                 255     


Ala Leu Ile Asn Ile Ala Arg Gly Ala Met Gly Ser Arg Pro Tyr Ile 
            260                 265                 270         


Phe Val Gly Ala Asn Ser Ser Glu Tyr Leu Gly Phe Val Ser Thr Phe 
        275                 280                 285             


Met Ala Ile Phe Ile Trp Gly Leu Ala Ala Trp Cys Tyr Cys Leu Ala 
    290                 295                 300                 


Met Val Ser Phe Leu Ala Gly Phe Phe Thr Arg Ala Pro Leu Lys Phe 
305                 310                 315                 320 


Ala Cys Gly Trp Phe Ala Phe Ile Phe Pro Asn Val Gly Phe Val Asn 
                325                 330                 335     


Cys Thr Ile Glu Ile Gly Lys Met Ile Asp Ser Lys Ala Phe Gln Met 
            340                 345                 350         


Phe Gly His Ile Ile Gly Val Ile Leu Cys Ile Gln Trp Ile Leu Leu 
        355                 360                 365             


Met Tyr Leu Met Val Arg Ala Phe Leu Val Asn Asp Leu Cys Tyr Pro 
    370                 375                 380                 


Gly Lys Asp Glu Asp Ala His Pro Pro Pro Lys Pro Asn Thr Gly Val 
385                 390                 395                 400 


Leu Asn Pro Thr Phe Pro Pro Glu Lys Ala Pro Ala Ser Leu Glu Lys 
                405                 410                 415     


Val Asp Thr His Val Thr Ser Thr Gly Gly Glu Ser Asp Pro Pro Ser 
            420                 425                 430         


Ser Glu His Glu Ser Val 
        435             


