                         SEQUENCE LISTING

<110>  National University of Ireland, Galway
 
<120>  Nucleic acid sequences based on the bacterial ssrA gene and its 
       tmRNA transcript for the molecular detection of S. aureus.

<130>  PR216PCT

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Amplification Primer

<400>  1
gacgttcatg gattcgaca                                                    19


<210>  2
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Amplification Primer

<400>  2
agacggcggg atttgaa                                                      17


<210>  3
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Amplification Primer

<400>  3
aattgtaata cgactcacta tagggagttc gtcatcaaca caca                        44


<210>  4
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Amplification Primer

<400>  4
cagaggtcct gatacaca                                                     18


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Amplification Primer

<400>  5
ttcgtcatca acacacaata cccaacttgg aatg                                   34


<210>  6
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Amplification Primer

<400>  6
cagaggtcct gatacacatc ttcaccagaa taaaattg                               38


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Amplification Primer

<400>  7
ctaacaataa cttagcagta gct                                               23


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Amplification Primer

<400>  8
gcagtgttta gcatatccta t                                                 21


<210>  9
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  9
accctccgac acg                                                          13


<210>  10
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  10
ctgttaggcg atgca                                                        15


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  11
cacataggaa atgctgtt                                                     18


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  12
ctattaaggt tgaatcgc                                                     18


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  13
ctagtttgat taagtttctt ct                                                22


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  14
gcatcatgaa aagtgataa                                                    19


<210>  15
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  15
acacgcttaa tgagct                                                       16


<210>  16
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  16
ctccgacacg cttaat                                                       16


<210>  17
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  17
tgtgtgttga tgacga                                                       16


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  18
tttgccagtt attataaact g                                                 21


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  19
actgcgaaat tattgtttg                                                    19


<210>  20
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  20
ttaggcagct actgc                                                        15


<210>  21
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  21
agagtgcgat taggc                                                        15


<210>  22
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  22
aatgctgtta ggcgat                                                       16


<210>  23
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  23
gaaatgctgt taggcg                                                       16


<210>  24
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  24
gaatcgcgtt aacagc                                                       16


<210>  25
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  25
taaggttgaa tcgcgt                                                       16


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  26
tcctattaag gttgaatcg                                                    19


<210>  27
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  27
acggcagtgt ttagc                                                        15


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  28
aagtttcttc taaacagact                                                   20


<210>  29
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  29
ccaacatgat gctagc                                                       16


<210>  30
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  30
ggtttcgcat catgaaa                                                      17


<210>  31
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  31
cgtgtgtagt ttatcgaa                                                     18


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  32
acacatcttt ctacgtgt                                                     18


<210>  33
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  33
aggtcctgat acacatc                                                      17


<210>  34
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  34
ggcgggattt gaacc                                                        15


<210>  35
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  35
ccagcaagtt tcttctaaac agactgctgg                                        30


<210>  36
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  36
ccgagtgaat gtatcccctg gactcgg                                           27


<210>  37
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  37
taacagcatt tcctatgtgc tgt                                               23


<210>  38
<211>  334
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  38
gggggtcccs gagctcatta agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt       60

ttataataac tggcaaatca aacaataatt tcgcagtagc tgcctaatcg cactctgcat      120

cgcctaacag catttcctat gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa      180

cactgccgtt tgaagtctgt ttagaagaaa cttaatcaag ctagcatcat gttggttgtt      240

tatcactttt catgatgcga aacctttcga taaactacac acgtagaaag atgtgtatca      300

ggacctctgg acgcgggttc aaatcccgcc gtct                                  334


<210>  39
<211>  334
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  39
gggggtcccs gagctcatta agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt       60

ttataataac tggcaaatca aacaataatt tcgcagtagc tgcctaatcg cactctgcat      120

cgcctaacag catttcctat gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa      180

cactgccgtt tgaagtctgt ttagaagaaa cttaatcaaa ctagcatcat gttggttgtt      240

tatcactttt catgatgcga aacctatcga taaactacac acgtagaaag atgtgtatca      300

ggacctttgg acgcgggttc aaatcccgcc gtct                                  334


<210>  40
<211>  334
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  40
gggggtcccs gagctcatta agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt       60

ttataataac tggcaaatca aacaataatt tcgcagtagc tgcctaatcg cactctgcat      120

cgcctaacag catttcctat gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa      180

cactgccgtt tgaagtctgt ttagaagaaa cttaatcaag ctagcatcat gttggttgtt      240

tatcactttt catgatgcga aacctttcga taaactacac acgtagaaag atgtgtatca      300

ggacctctgg acgcgggttc aaatcccgcc gtct                                  334


<210>  41
<211>  306
<212>  DNA
<213>  Staphylococcus aureus

<400>  41
ggagggttgt cttcgtcatc aacacacaca gtttataata actggcaaaa caaacaataa       60

tttcgcagta gctgcctaat cgcactctgc atcgcctaac agcatttcct atgtgctgtt      120

aacgcgattc aaccttaata ggatatgcta aacactgccg tttgaagtct gtttagaaga      180

aacttaatca agctagcatc atgttggttg tttatcactt ttcatgatgc gaaacctttc      240

gataaactac acacgtagaa agatgtgtat caggacctct ggacgcgggt tcaaatcccg      300

ccgtct                                                                 306


<210>  42
<211>  334
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  42
gggggtcccs gagctcatta agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt       60

ttataataac tggcaaaaca aacaataatt tcgcagtagc tgcctaatcg cactctgcat      120

cgcctaacag catttcctat gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa      180

cactgccgtt tgaagtctgt ttagaagaaa cttaatcaag ctagcatcat gttggttgtt      240

tatcactttt catgatgcga aacctttcga taaactacac acgtagaaag atgtgtatca      300

ggacctctgg acgcgggttc aaatcccgcc gtct                                  334


<210>  43
<211>  334
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  43
gggggtcccs gagctcatta agcgtgtcgg agggttgtct tcgtcatcaa cacacacagt       60

ttataataac tggcaaatca aacaataatt tcgcagtagc tgcctaatcg cactctgcat      120

cgcctaacag catttcctat gtgctgttaa cgcgattcaa ccttaatagg atatgctaaa      180

cactgccgtt tgaagtctgt ttagaagaaa cttaatcaaa ctagcatcat gttggttgtt      240

tatcactttt catgatgcga aacctatcga taaactacac acgtagaaag atgtgtatca      300

ggacctttgg acgcgggttc aaatcccgcc gtct                                  334


<210>  44
<211>  315
<212>  DNA
<213>  Staphylococcus aureus

<400>  44
aagcgtgtcg gagggttgtc tccgatatca acacatttca gtaaatataa ctgacaaatc       60

aaacaataat ttcgcagtag ctgcgtaata gcacactgca tcgcctaaca gcatctccta      120

cgtgctgtta acgcgattca accctagtag gatatgctaa acactgccgt ttgaagtctg      180

tttagatgaa tactaatcaa actagcataa tactggttgt ctattgctta ctattatgcg      240

aaatgaatca atagactaca cacgtagaaa ggtgtgtatc aggacctctg gacgcgggtt      300

caaatcccgc cgtct                                                       315


<210>  45
<211>  302
<212>  DNA
<213>  Staphylococcus caseolyticus


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  45
cgtgtcggag ggttgtcttc gtcatcaaca caacagttta taataactgg caaaacaaac       60

aatttcgcag tagctgccta atcgcactct gcacctctct cacgcatcgc ctatgtgcct      120

gatgtagagg ttcaacttaa gtaggatacg ctgtacacct ccgcttgggg tctgtacaga      180

agagattaat cgagctagtt attaaccagg atgtctgttt tcaggttaat agcgaaactt      240

aataacagga ctacacacgt agaagtatca gctgcaggat ccttggacsy ggttcgaytc      300

cc                                                                     302


<210>  46
<211>  331
<212>  DNA
<213>  Staphylococcus chromogenes


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  46
gggggtccsa gagcttatga agcgtgtcgg agggttgtct ccgcaaaaac acatcagttt       60

ataataactg gcaaaactaa caataactta gcagtagctg cctaatcgcg ctctgcatca      120

cctaatcgca tcgcctatgt gcgattaacg tgattcaact ttagtaggat acgctcgtca      180

ctgccgtttg aagtctgacg agaagagatg aatcaaactc gcataatgtc ggttgtctgt      240

ttctaaacat tatgctaaat ttctaaacag actacacacg tagaaacatt tgtatcagga      300

cctttggacg cgggttcaaa tcccgccgtc t                                     331


<210>  47
<211>  334
<212>  DNA
<213>  Staphylococcus epidermidis


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  47
gggggtyccs gagcttatta agcgtgtcgg agggttggct ccgtcatcaa cacatttcgg       60

ttaaatataa ctgacaaatc aaacaataat ttcgcagtag ctgcgtaata gccactgcat      120

cgcctaacag catctcctac gtgctgttaa cgcgattcaa ccctagtagg atatgctaaa      180

cactgccgct tgaagtctgt ttagatgaaa tataatcaag ctagtatcat gttggttgtt      240

tattgcttag catgatgcga aaattatcaa taaactacac acgtagaaag atttgtatca      300

ggacctctgg acgcgggttc aaatcccgcc gtct                                  334


<210>  48
<211>  334
<212>  DNA
<213>  Staphylococcus epidermidis


<220>
<221>  misc_feature
<223>  N or x= any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t,
       h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific 
       degeneracy options are indicated in parenthesis: e.g.: (a/g) is 
       either A or G

<400>  48
gggggtcccs gagcttatta agcgtgtcgg agggttggct ccgtcatcaa cacatttcgg       60

ttaaatataa ctgacaaatc aaacaataat ttcgcagtag ctgcgtaata gccactgcat      120

cgcctaacag catctcctac gtgctgttaa cgcgattcaa ccctagtagg atatgctaaa      180

cactgccgct tgaagtctgt ttagatgaaa tataatcaag ctagtatcat gttggttgtt      240

tattgcttag catgatgcga aaattatcaa taaactacac acgtagaaag atttgtatca      300

ggacctctgg acgcgggttc aaatcccgcc gtct                                  334


