
                                SEQUENCE LISTING

<110> Antigenics, Inc.
      LeClair, Kenneth P.
      Tomlinson, Andrew J.

<120> METHODS FOR PREPARING AND USING
  MULTICHAPERONE ANTIGEN COMPLEXES
  

<130> 8449-513-228

<140>
<141>

<150> 61/211,850
<151> 2009-04-03

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 124
<212> PRT
<213> Homo sapiens

<220> 
<223> human HOP TPR1

<400> 1
Met Glu Gln Val Asn Glu Leu Lys Glu Lys Gly Asn Lys Ala Leu Ser
 1               5                  10                  15      
Val Gly Asn Ile Asp Asp Ala Leu Gln Cys Tyr Ser Glu Ala Ile Lys
            20                  25                  30          
Leu Asp Pro His Asn His Val Leu Tyr Ser Asn Arg Ser Ala Ala Tyr
        35                  40                  45              
Ala Lys Lys Gly Asp Tyr Gln Lys Ala Tyr Glu Asp Gly Cys Lys Thr
    50                  55                  60                  
Val Asp Leu Lys Pro Asp Trp Gly Lys Gly Tyr Ser Arg Lys Ala Ala
65                  70                  75                  80  
Ala Leu Glu Phe Leu Asn Arg Phe Glu Glu Ala Lys Arg Thr Tyr Glu
                85                  90                  95      
Glu Gly Leu Lys His Glu Ala Asn Asn Pro Gln Leu Lys Glu Gly Leu
            100                 105                 110         
Gln Asn Met Glu Ala Arg His His His His His Gln
        115                 120                 


<210> 2
<211> 137
<212> PRT
<213> Homo sapiens

<220> 
<223> human HOP TPR2a

<400> 2
Met Lys Gln Ala Leu Lys Glu Lys Glu Leu Gly Asn Asp Ala Tyr Lys
 1               5                  10                  15      
Lys Lys Asp Phe Asp Thr Ala Leu Lys His Tyr Asp Lys Ala Lys Glu
            20                  25                  30          
Leu Asp Pro Thr Asn Met Thr Tyr Ile Thr Asn Gln Ala Ala Val Tyr
        35                  40                  45              
Phe Glu Lys Gly Asp Tyr Asn Lys Cys Arg Glu Leu Cys Glu Lys Ala
    50                  55                  60                  
Ile Glu Val Gly Arg Glu Asn Arg Glu Asp Tyr Arg Gln Ile Ala Lys
65                  70                  75                  80  
Ala Tyr Ala Arg Ile Gly Asn Ser Tyr Phe Lys Glu Glu Lys Tyr Lys
                85                  90                  95      
Asp Ala Ile His Phe Tyr Asn Lys Ser Leu Ala Glu His Arg Thr Pro
            100                 105                 110         
Asp Val Leu Lys Lys Cys Gln Gln Ala Glu Lys Ile Leu Lys Glu Gln
        115                 120                 125             
Glu Arg Leu His His His His His Gln
    130                 135         


<210> 3
<211> 358
<212> PRT
<213> Homo sapiens

<220> 
<223> human HOP TPR1/2a

<400> 3
Met Glu Gln Val Asn Glu Leu Lys Glu Lys Gly Asn Lys Ala Leu Ser
 1               5                  10                  15      
Val Gly Asn Ile Asp Asp Ala Leu Gln Cys Tyr Ser Glu Ala Ile Lys
            20                  25                  30          
Leu Asp Pro His Asn His Val Leu Tyr Ser Asn Arg Ser Ala Ala Tyr
        35                  40                  45              
Ala Lys Lys Gly Asp Tyr Gln Lys Ala Tyr Glu Asp Gly Cys Lys Thr
    50                  55                  60                  
Val Asp Leu Lys Pro Asp Trp Gly Lys Gly Tyr Ser Arg Lys Ala Ala
65                  70                  75                  80  
Ala Leu Glu Phe Leu Asn Arg Phe Glu Glu Ala Lys Arg Thr Tyr Glu
                85                  90                  95      
Glu Gly Leu Lys His Glu Ala Asn Asn Pro Gln Leu Lys Glu Gly Leu
            100                 105                 110         
Gln Asn Met Glu Ala Arg Leu Ala Glu Arg Lys Phe Met Asn Pro Phe
        115                 120                 125             
Asn Met Pro Asn Leu Tyr Gln Lys Leu Glu Ser Asp Pro Arg Thr Arg
    130                 135                 140                 
Thr Leu Leu Ser Asp Pro Thr Tyr Arg Glu Leu Ile Glu Gln Leu Arg
145                 150                 155                 160 
Asn Lys Pro Ser Asp Leu Gly Thr Lys Leu Gln Asp Pro Arg Ile Met
                165                 170                 175     
Thr Thr Leu Ser Val Leu Leu Gly Val Asp Leu Gly Ser Met Asp Glu
            180                 185                 190         
Glu Glu Glu Ile Ala Thr Pro Pro Pro Pro Pro Pro Pro Lys Lys Glu
        195                 200                 205             
Thr Lys Pro Glu Pro Met Glu Glu Asp Leu Pro Glu Asn Lys Lys Gln
    210                 215                 220                 
Ala Leu Lys Glu Lys Glu Leu Gly Asn Asp Ala Tyr Lys Lys Lys Asp
225                 230                 235                 240 
Phe Asp Thr Ala Leu Lys His Tyr Asp Lys Ala Lys Glu Leu Asp Pro
                245                 250                 255     
Thr Asn Met Thr Tyr Ile Thr Asn Gln Ala Ala Val Tyr Phe Glu Lys
            260                 265                 270         
Gly Asp Tyr Asn Lys Cys Arg Glu Leu Cys Glu Lys Ala Ile Glu Val
        275                 280                 285             
Gly Arg Glu Asn Arg Glu Asp Tyr Arg Gln Ile Ala Lys Ala Tyr Ala
    290                 295                 300                 
Arg Ile Gly Asn Ser Tyr Phe Lys Glu Glu Lys Tyr Lys Asp Ala Ile
305                 310                 315                 320 
His Phe Tyr Asn Lys Ser Leu Ala Glu His Arg Thr Pro Asp Val Leu
                325                 330                 335     
Lys Lys Cys Gln Gln Ala Glu Lys Ile Leu Lys Glu Gln Glu Arg Leu
            340                 345                 350         
His His His His His His
        355             


<210> 4
<211> 125
<212> PRT
<213> Homo sapiens

<220> 
<223> human HOP TPR2b

<400> 4
Ala Tyr Ile Asn Pro Asp Leu Ala Leu Glu Glu Lys Asn Lys Gly Asn
 1               5                  10                  15      
Glu Cys Phe Gln Lys Gly Asp Tyr Pro Gln Ala Met Lys His Tyr Thr
            20                  25                  30          
Glu Ala Ile Lys Arg Asn Pro Lys Asp Ala Lys Leu Tyr Ser Asn Arg
        35                  40                  45              
Ala Ala Cys Tyr Thr Lys Leu Leu Glu Phe Gln Leu Ala Leu Lys Asp
    50                  55                  60                  
Cys Glu Glu Cys Ile Gln Leu Glu Pro Thr Phe Ile Lys Gly Tyr Thr
65                  70                  75                  80  
Arg Lys Ala Ala Ala Leu Glu Ala Met Lys Asp Tyr Thr Lys Ala Met
                85                  90                  95      
Asp Val Tyr Gln Lys Ala Leu Asp Leu Asp Ser Ser Cys Lys Glu Ala
            100                 105                 110         
Ala Asp Gly Tyr Gln Arg Cys Met Met Ala Gln Tyr Asn
        115                 120                 125 


<210> 5
<211> 375
<212> DNA
<213> Artificial Sequence

<220> 
<223> cDNA construct of HOP TPR1

<400> 5
atggagcagg tcaatgagct gaaggagaaa ggcaacaagg ccctgagcgt gggtaacatc 60
gatgatgcct tacagtgcta ctccgaagct attaagctgg atccccacaa ccacgtgctg 120
tacagcaacc gttctgctgc ctatgccaag aaaggagact accagaaggc ttatgaggat 180
ggctgcaaga ctgtcgacct aaagcctgac tggggcaagg gctattcacg aaaagcagca 240
gctctagagt tcttaaaccg ctttgaagaa gccaagcgaa cctatgagga gggcttaaaa 300
cacgaggcaa ataaccctca actgaaagag ggtttacaga atatggaggc caggcaccac 360
catcaccatc agtag                                                  375

<210> 6
<211> 414
<212> DNA
<213> Artificial Sequence

<220> 
<223> cDNA construct of HOP TPR2a

<400> 6
atgaagcagg cactgaaaga aaaagagctg gggaacgatg cctacaagaa gaaagacttt 60
gacacagcct tgaagcatta cgacaaagcc aaggagctgg accccactaa catgacttac 120
attaccaatc aagcagcggt atactttgaa aagggcgact acaataagtg ccgggagctt 180
tgtgagaagg ccattgaagt ggggagagaa aaccgagaag actatcgaca gattgccaaa 240
gcgtatgctc gaattggcaa ctcctacttc aaagaagaaa agtacaagga tgccatccat 300
ttctataaca agtctctggc agagcaccga accccagatg tgctcaagaa atgccagcag 360
gcagagaaaa tcctgaagga gcaagagcgg ctgcaccacc atcaccatca gtag       414

<210> 7
<211> 1077
<212> DNA
<213> Artificial Sequence

<220> 
<223> cDNA construct of HOP TPR1/2a

<400> 7
atggagcagg tcaatgagct gaaggagaaa ggcaacaagg ccctgagcgt gggtaacatc 60
gatgatgcct tacagtgcta ctccgaagct attaagctgg atccccacaa ccacgtgctg 120
tacagcaacc gttctgctgc ctatgccaag aaaggagact accagaaggc ttatgaggat 180
ggctgcaaga ctgtcgacct aaagcctgac tggggcaagg gctattcacg aaaagcagca 240
gctctagagt tcttaaaccg ctttgaagaa gccaagcgaa cctatgagga gggcttaaaa 300
cacgaggcaa ataaccctca actgaaagag ggtttacaga atatggaggc caggttggca 360
gagagaaaat tcatgaaccc tttcaacatg cctaatctgt atcagaagtt ggagagtgat 420
cccaggacaa ggacactact cagtgatcct acctaccggg agctgataga gcagctacga 480
aacaagcctt ctgacctggg cacgaaacta caagatcccc ggatcatgac cactctcagc 540
gtcctccttg gggtcgatct gggcagtatg gatgaggagg aagagattgc aacacctcca 600
ccaccacccc ctcccaaaaa ggagaccaag ccagagccaa tggaagaaga tcttccagag 660
aataagaagc aggcactgaa agaaaaagag ctggggaacg atgcctacaa gaagaaagac 720
tttgacacag ccttgaagca ttacgacaaa gccaaggagc tggaccccac taacatgact 780
tacattacca atcaagcagc ggtatacttt gaaaagggcg actacaataa gtgccgggag 840
ctttgtgaga aggccattga agtggggaga gaaaaccgag aagactatcg acagattgcc 900
aaagcgtatg ctcgaattgg caactcctac ttcaaagaag aaaagtacaa ggatgccatc 960
catttctata acaagtctct ggcagagcac cgaaccccag atgtgctcaa gaaatgccag 1020
caggcagaga aaatcctgaa ggagcaagag cggctgcacc accatcacca tcactag    1077

<210> 8
<211> 543
<212> PRT
<213> Homo sapiens

<220> 
<223> human HOP protein (full length)(GenBank Accession No. P31948)

<400> 8
Met Glu Gln Val Asn Glu Leu Lys Glu Lys Gly Asn Lys Ala Leu Ser
 1               5                  10                  15      
Val Gly Asn Ile Asp Asp Ala Leu Gln Cys Tyr Ser Glu Ala Ile Lys
            20                  25                  30          
Leu Asp Pro His Asn His Val Leu Tyr Ser Asn Arg Ser Ala Ala Tyr
        35                  40                  45              
Ala Lys Lys Gly Asp Tyr Gln Lys Ala Tyr Glu Asp Gly Cys Lys Thr
    50                  55                  60                  
Val Asp Leu Lys Pro Asp Trp Gly Lys Gly Tyr Ser Arg Lys Ala Ala
65                  70                  75                  80  
Ala Leu Glu Phe Leu Asn Arg Phe Glu Glu Ala Lys Arg Thr Tyr Glu
                85                  90                  95      
Glu Gly Leu Lys His Glu Ala Asn Asn Pro Gln Leu Lys Glu Gly Leu
            100                 105                 110         
Gln Asn Met Glu Ala Arg Leu Ala Glu Arg Lys Phe Met Asn Pro Phe
        115                 120                 125             
Asn Met Pro Asn Leu Tyr Gln Lys Leu Glu Ser Asp Pro Arg Thr Arg
    130                 135                 140                 
Thr Leu Leu Ser Asp Pro Thr Tyr Arg Glu Leu Ile Glu Gln Leu Arg
145                 150                 155                 160 
Asn Lys Pro Ser Asp Leu Gly Thr Lys Leu Gln Asp Pro Arg Ile Met
                165                 170                 175     
Thr Thr Leu Ser Val Leu Leu Gly Val Asp Leu Gly Ser Met Asp Glu
            180                 185                 190         
Glu Glu Glu Ile Ala Thr Pro Pro Pro Pro Pro Pro Pro Lys Lys Glu
        195                 200                 205             
Thr Lys Pro Glu Pro Met Glu Glu Asp Leu Pro Glu Asn Lys Lys Gln
    210                 215                 220                 
Ala Leu Lys Glu Lys Glu Leu Gly Asn Asp Ala Tyr Lys Lys Lys Asp
225                 230                 235                 240 
Phe Asp Thr Ala Leu Lys His Tyr Asp Lys Ala Lys Glu Leu Asp Pro
                245                 250                 255     
Thr Asn Met Thr Tyr Ile Thr Asn Gln Ala Ala Val Tyr Phe Glu Lys
            260                 265                 270         
Gly Asp Tyr Asn Lys Cys Arg Glu Leu Cys Glu Lys Ala Ile Glu Val
        275                 280                 285             
Gly Arg Glu Asn Arg Glu Asp Tyr Arg Gln Ile Ala Lys Ala Tyr Ala
    290                 295                 300                 
Arg Ile Gly Asn Ser Tyr Phe Lys Glu Glu Lys Tyr Lys Asp Ala Ile
305                 310                 315                 320 
His Phe Tyr Asn Lys Ser Leu Ala Glu His Arg Thr Pro Asp Val Leu
                325                 330                 335     
Lys Lys Cys Gln Gln Ala Glu Lys Ile Leu Lys Glu Gln Glu Arg Leu
            340                 345                 350         
Ala Tyr Ile Asn Pro Asp Leu Ala Leu Glu Glu Lys Asn Lys Gly Asn
        355                 360                 365             
Glu Cys Phe Gln Lys Gly Asp Tyr Pro Gln Ala Met Lys His Tyr Thr
    370                 375                 380                 
Glu Ala Ile Lys Arg Asn Pro Lys Asp Ala Lys Leu Tyr Ser Asn Arg
385                 390                 395                 400 
Ala Ala Cys Tyr Thr Lys Leu Leu Glu Phe Gln Leu Ala Leu Lys Asp
                405                 410                 415     
Cys Glu Glu Cys Ile Gln Leu Glu Pro Thr Phe Ile Lys Gly Tyr Thr
            420                 425                 430         
Arg Lys Ala Ala Ala Leu Glu Ala Met Lys Asp Tyr Thr Lys Ala Met
        435                 440                 445             
Asp Val Tyr Gln Lys Ala Leu Asp Leu Asp Ser Ser Cys Lys Glu Ala
    450                 455                 460                 
Ala Asp Gly Tyr Gln Arg Cys Met Met Ala Gln Tyr Asn Arg His Asp
465                 470                 475                 480 
Ser Pro Glu Asp Val Lys Arg Arg Ala Met Ala Asp Pro Glu Val Gln
                485                 490                 495     
Gln Ile Met Ser Asp Pro Ala Met Arg Leu Ile Leu Glu Gln Met Gln
            500                 505                 510         
Lys Asp Pro Gln Ala Leu Ser Glu His Leu Lys Asn Pro Val Ile Ala
        515                 520                 525             
Gln Lys Ile Gln Lys Leu Met Asp Val Gly Leu Ile Ala Ile Arg
    530                 535                 540             


<210> 9
<211> 104
<212> PRT
<213> Homo sapiens

<220> 
<223> DP1 of human HOP protein

<400> 9
Leu Ala Glu Arg Lys Phe Met Asn Pro Phe Asn Met Pro Asn Leu Tyr
 1               5                  10                  15      
Gln Lys Leu Glu Ser Asp Pro Arg Thr Arg Thr Leu Leu Ser Asp Pro
            20                  25                  30          
Thr Tyr Arg Glu Leu Ile Glu Gln Leu Arg Asn Lys Pro Ser Asp Leu
        35                  40                  45              
Gly Thr Lys Leu Gln Asp Pro Arg Ile Met Thr Thr Leu Ser Val Leu
    50                  55                  60                  
Leu Gly Val Asp Leu Gly Ser Met Asp Glu Glu Glu Glu Ile Ala Thr
65                  70                  75                  80  
Pro Pro Pro Pro Pro Pro Pro Lys Lys Glu Thr Lys Pro Glu Pro Met
                85                  90                  95      
Glu Glu Asp Leu Pro Glu Asn Lys
            100                 


<210> 10
<211> 66
<212> PRT
<213> Homo sapiens

<220> 
<223> DP2 of human HOP protein

<400> 10
Arg His Asp Ser Pro Glu Asp Val Lys Arg Arg Ala Met Ala Asp Pro
 1               5                  10                  15      
Glu Val Gln Gln Ile Met Ser Asp Pro Ala Met Arg Leu Ile Leu Glu
            20                  25                  30          
Gln Met Gln Lys Asp Pro Gln Ala Leu Ser Glu His Leu Lys Asn Pro
        35                  40                  45              
Val Ile Ala Gln Lys Ile Gln Lys Leu Met Asp Val Gly Leu Ile Ala
    50                  55                  60                  
Ile Arg
65      

