                         SEQUENCE LISTING

<110>  University of Utah Research Foundation
       
       BURROWS, CYNTHIA
       KANNAN, ARUNKUMAR
       BEAL, PETER A.
 
<120>  METHODS AND COMPOSITIONS RELATED TO MODIFIED GUANINE BASES FOR 
       CONTROLLING OFF TARGET EFFECTS IN RNA INTERFERENCE

<130>  21101.0182P1

<140>  
<141>  2010-03-23

<150>  61/162507
<151>  2009-03-23

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  1
ggaaaugcaa gagaaacugt t                                                 21


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  2
dgtccuuuac guucucuuug ac                                                22


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  3
caguuucucu ugcauuucct t                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  X is subsituted with N. X is 8-oxo-2'-deoxyguanosine

<400>  4
caguuucucu ugcaunucct t                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X is substituted with N. X is 8-oxo-2'-deoxyguanosine

<400>  5
caguuucucu ngcauuucct t                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  X is subsituted with N. X is 8-oxo-2'-deoxyguanosine

<400>  6
cagnuucucu ugcauuucct t                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  7
ggaaaugcaa gagaaacugt t                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  8
ttccuuuacg uucucuuuga c                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  9
ggaaaugcaa gagaaccugt t                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  X is substituted with N. X is 8-oxo-2'-deoxyguanosine

<400>  10
ttccuuuacg uucucuunga c                                                 21


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  11
ggaaaugccc agagaaacug tt                                                22


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X is substituted with N. N is 8-oxo-2'-deoxyguanosine

<400>  12
ttccuuuacg nucucuuuga c                                                 21


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  13
ggacaugcaa gagaaacugt t                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  X is substituted with N. N is 8-oxo-2'-deoxyguanosine

<400>  14
ttccunuacg uucucuuuga c                                                 21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (4)..(16)
<223>  X is substituted with N at posititons 4,11, 16. N is 
       N2-alkyl-oxo-2'-deoxyguanosine phosphoramidite

<400>  15
cagnuucucu ngcaunucct t                                                 21


<210>  16
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  16
atcgctcgag gcacaggaaa tgcaagagaa actgcagaag cggccgctgg c                51


<210>  17
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  17
tagcgagctc cgtgtcgttt acgttctctt tgacgtcttc gccggcgacc g                51


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  18
ggaaaugcaa gagaaacugt t                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  19
ttccuuuacg uucucuuuga c                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  20
ggaaaugcaa gagaaacugt t                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  21
ttccuuuacg uucucuuuga c                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  22
ggacaugcaa gagaaacugt t                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  X is substitututed with N. N is 8-oxo-2'-deoxyguanosine

<400>  23
ttccunuacg uucucuuuga c                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  24
ggaaaugcca gagaaacugt t                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X is substituted with N. N is 8-oxo-2'-deoxyguanosine

<400>  25
ttccuuuacg nucucuuuga c                                                 21


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  26
ggaaaugcaa gagaaccugt t                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  X is substituted with N. N is 8-oxo-2'-deoxyguanosine

<400>  27
ttccuuuacg uucucuunga c                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  28
ggaaaugcaa gagaaacugt t                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  29
ttccuuuacg uucucuuuga c                                                 21


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  30
ggacaugcaa gagaaacugt t                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  X is substituted with N. N is N2-propyl-oxo-2'-deoxyguanosine

<400>  31
ttccunuacg uucucuuuga c                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  32
ggaaaugcca gagaaacugt t                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X is substituted with N. N is N2-propyl-oxo-2'-deoxyguanosine

<400>  33
ttccuuuacg nucucuuuga c                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  34
ggaaaugcaa gagaaccugt t                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  X is substituted with N. N is N2-propyl-oxo-2'-deoxyguanosine

<400>  35
ttccuuuacg uucucuunga c                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  36
ggaaaugcaa gagaaacugt t                                                 21


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  37
ttccuuuacg uucucuuuga c                                                 21


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  38
ggacaugcaa gagaaacugt t                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  X is substituted with N. N is N2-benzyl-oxo-2'-deoxyguanosine

<400>  39
ttccunuacg uucucuuuga c                                                 21


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  40
ggaaaugcca gagaaacugt t                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X is substituted with N. N is N2-benzyl-oxo-2'-deoxyguanosine

<400>  41
ttccuuuacg nucucuuuga c                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  42
ggaaaugcaa gagaaccugt t                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  X is substituted with N. N is N2-benzyl-oxo-2'-deoxyguanosine

<400>  43
ttccuuuacg uucucuunga c                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  44
ggaaaugcaa gagaaacugt t                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  45
ttccuuuacg uucucuuuga c                                                 21


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(18)
<223>  X is substituted with N at positions 11, 18. N is 
       8-oxo-2'-deoxyguanosine

<400>  46
ttccuuuacg nucucuunga c                                                 21


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  X is substituted with N at positions 6,11. N is 
       8-oxo-2'-deoxyguanosine

<400>  47
ttccunuacg nucucuuuga c                                                 21


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(18)
<223>  X is substituted with N at positions 6, 18. N is 
       8-oxo-2'-deoxyguanosine

<400>  48
ttccunuacg uucucuunga c                                                 21


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(18)
<223>  X is substituted with N at positions 6, 11, 18. N is 
       8-oxo-2'-deoxyguanosine

<400>  49
ttccunuacg nucucuunga c                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  50
ggaaaugcaa gagaaacugt t                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct

<400>  51
ttccuuuacg uucucuuuga c                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (11)..(18)
<223>  X is substituted with N at positions 11, 18. N is 
       N2-propyl-oxo-2'-deoxyguanosine

<400>  52
ttccuuuacg nucucuunga c                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(11)
<223>  X is substituted with N at positions 6, 11. N is 
       N2-propyl-oxo-2'-deoxyguanosine

<400>  53
ttccunuacg nucucuuuga c                                                 21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(18)
<223>  X is substituted with N at positions 6, 18. N is 
       N2-propyl-oxo-2'-deoxyguanosine

<400>  54
ttccunuacg uucucuunga c                                                 21


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Note = Synthetic Construct


<220>
<221>  misc_feature
<222>  (6)..(18)
<223>  X is substituted with N at positions 6, 11, 18. N is 
       N2-propyl-oxo-2'-deoxyguanosine

<400>  55
ttccunuacg nucucuunga c                                                 21


