                              Sequence Listing

<110> GENENTECH, INC.
      Liang, Wei-Ching     
      Plowman, Gregory D.
      Wu, Yan
      Ye, Weilan
      
<120> NOVEL ANTI-ALPHA5BETA1 ANTIBODIES AND USES THEREOF

<130> P4170R1 WO


<150> US 61/163,241
<151> 2009-03-25

<160> 40

<210> 1
<211> 110
<212> PRT
<213> Humanized

<220>
<221> Variant
<222> 22-36
<223> X is any amino acid

<220>
<221> Unsure
<222> 22-36,52-62,95-105
<223> Unknown amino acid

<220>
<221> Variant
<222> 52-62
<223> X is any amino acid

<220>
<221> Variant
<222> 95-105
<223> X is any amino acid

<400> 1
 Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln
   1               5                  10                  15

 Thr Val Arg Ile Thr Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                  20                  25                  30

 Xaa Xaa Xaa Xaa Xaa Xaa Trp Tyr Gln Gln Lys Pro Gly Gln Ala
                  35                  40                  45

 Pro Val Leu Val Ile Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                  50                  55                  60

 Xaa Xaa Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn
                  65                  70                  75

 Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala
                  80                  85                  90

 Asp Tyr Tyr Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                  95                 100                 105

 Phe Gly Gly Gly Thr
                 110

<210> 2
<211> 106
<212> PRT
<213> Cricetulus griseus

<400> 2
 Gln Pro Val Leu Thr Gln Ser Pro Ser Ala Ser Ala Ser Leu Gly
   1               5                  10                  15

 Asn Ser Val Lys Ile Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln His Pro Asp Lys Ala Pro Lys
                  35                  40                  45

 Tyr Val Met Tyr Leu Asn Ser Asp Gly Ser His Asn Lys Gly Asp
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala His Arg
                  65                  70                  75

 Tyr Leu Ser Ile Ser Asn Ile Gln Pro Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Phe Cys Gly Ser Ser Tyr Ser Ser Gly Tyr Val Phe Gly Ser Gly
                  95                 100                 105

 Thr
    

<210> 3
<211> 106
<212> PRT
<213> Humanized

<400> 3
 Asp Ile Val Leu Thr Gln Ser Pro Ser Ala Ser Ala Ser Leu Gly
   1               5                  10                  15

 Asn Ser Val Lys Ile Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln His Pro Asp Lys Ala Pro Lys
                  35                  40                  45

 Tyr Val Met Tyr Leu Asn Ser Asp Gly Ser His Asn Lys Gly Asp
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala His Arg
                  65                  70                  75

 Tyr Leu Ser Ile Ser Asn Ile Gln Pro Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Phe Cys Gly Ser Ser Tyr Ser Ser Gly Tyr Val Phe Gly Ser Gly
                  95                 100                 105

 Thr
    

<210> 4
<211> 106
<212> PRT
<213> Humanized

<400> 4
 Glu Pro Val Leu Thr Gln Ser Pro Ser Ala Ser Ala Ser Leu Gly
   1               5                  10                  15

 Ala Ser Val Lys Leu Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln Gln Pro Gly Lys Ala Pro Arg
                  35                  40                  45

 Tyr Leu Met Tyr Leu Asn Ser Asp Gly Ser His Asn Lys Gly Asp
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala Asp Arg
                  65                  70                  75

 Tyr Leu Thr Ile Ser Asn Leu Gln Ser Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Tyr Cys Gly Ser Ser Tyr Ser Ser Gly Tyr Val Phe Gly Gly Gly
                  95                 100                 105

 Thr
    

<210> 5
<211> 106
<212> PRT
<213> Humanized

<400> 5
 Glu Pro Val Leu Thr Gln Ser Pro Ser Ala Ser Ala Ser Leu Gly
   1               5                  10                  15

 Ala Ser Val Lys Leu Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln Gln Pro Gly Lys Ala Pro Arg
                  35                  40                  45

 Tyr Leu Met Tyr Leu Asn Ser Asp Gly Ser His Asn Lys Gly Asp
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala Asp Arg
                  65                  70                  75

 Tyr Leu Thr Ile Ser Asn Leu Gln Ser Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Tyr Cys Ala Ala Tyr Tyr Ala Tyr Gly Tyr Val Phe Gly Gly Gly
                  95                 100                 105

 Thr
    

<210> 6
<211> 106
<212> PRT
<213> Humanized

<400> 6
 Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly
   1               5                  10                  15

 Gln Thr Val Arg Ile Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val
                  35                  40                  45

 Tyr Leu Met Tyr Leu Asn Ser Ser Gly Ser His Asn Lys Gly Ser
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala Thr Arg
                  65                  70                  75

 Ser Leu Thr Ile Thr Asn Ala Gln Ala Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Tyr Cys Ala Ala Tyr Tyr Ala Tyr Gly Tyr Val Phe Gly Gly Gly
                  95                 100                 105

 Thr
    

<210> 7
<211> 106
<212> PRT
<213> Humanized

<400> 7
 Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly
   1               5                  10                  15

 Gln Thr Val Arg Ile Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val
                  35                  40                  45

 Leu Val Ile Tyr Leu Asn Ser Ser Gly Ser His Asn Lys Gly Ser
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala Thr Arg
                  65                  70                  75

 Ser Leu Thr Ile Thr Asn Ala Gln Ala Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Tyr Cys Ala Ala Tyr Tyr Ala Tyr Gly Tyr Val Phe Gly Gly Gly
                  95                 100                 105

 Thr
    

<210> 8
<211> 106
<212> PRT
<213> Humanized

<400> 8
 Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly
   1               5                  10                  15

 Gln Thr Val Arg Ile Thr Cys Thr Leu Ser Ser Gln His Ser Thr
                  20                  25                  30

 Tyr Thr Ile Gly Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val
                  35                  40                  45

 Tyr Leu Met Tyr Leu Asn Ser Asp Ser Ser His Asn Lys Gly Ser
                  50                  55                  60

 Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Ala Thr Arg
                  65                  70                  75

 Ser Leu Thr Ile Thr Asn Ala Gln Ala Glu Asp Glu Ala Asp Tyr
                  80                  85                  90

 Tyr Cys Ala Ala Tyr Tyr Ala Tyr Gly Tyr Val Phe Gly Gly Gly
                  95                 100                 105

 Thr
    

<210> 9
<211> 114
<212> PRT
<213> Cricetulus griseus

<400> 9
 Glu Val His Leu Val Glu Ser Gly Gly Asp Leu Val Gln Pro Gly
   1               5                  10                  15

 Ser Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser
                  20                  25                  30

 Asn Arg Trp Ile Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Gly Ile Lys Thr Lys Pro Asn Ile Tyr Ala Thr
                  50                  55                  60

 Glu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
                  65                  70                  75

 Asp Ser Lys Asn Ser Leu Tyr Leu Gln Met Asn Thr Leu Arg Val
                  80                  85                  90

 Asp Asp Thr Ala Ile Tyr Tyr Cys Thr Ser Leu Thr Gly Met Arg
                  95                 100                 105

 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr
                 110                

<210> 10
<211> 116
<212> PRT
<213> Humanized

<220>
<221> Variant
<222> 26-35
<223> X is any amino acid

<220>
<221> Unsure
<222> 26-35,52-70,103-111
<223> Unknown amino acid

<220>
<221> Variant
<222> 52-70
<223> X is any amino acid

<220>
<221> Variant
<222> 103-111
<223> X is any amino acid

<400> 10
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Xaa Xaa Xaa Xaa Xaa
                  20                  25                  30

 Xaa Xaa Xaa Xaa Xaa Trp Asn Trp Val Arg Gln Ala Pro Gly Lys
                  35                  40                  45

 Gly Leu Glu Trp Val Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                  50                  55                  60

 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Phe Thr Ile Ser
                  65                  70                  75

 Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu
                  80                  85                  90

 Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Xaa Xaa Xaa
                  95                 100                 105

 Xaa Xaa Xaa Xaa Xaa Xaa Trp Gly Gln Gly Thr
                 110                 115    

<210> 11
<211> 114
<212> PRT
<213> Humanized

<400> 11
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser
                  20                  25                  30

 Asn Arg Trp Ile Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Gly Ile Lys Thr Lys Pro Asn Ile Tyr Ala Thr
                  50                  55                  60

 Glu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
                  65                  70                  75

 Asp Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala
                  80                  85                  90

 Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Leu Thr Gly Met Arg
                  95                 100                 105

 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr
                 110                

<210> 12
<211> 114
<212> PRT
<213> Humanized

<400> 12
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser
                  20                  25                  30

 Asn Arg Trp Ile Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Gly Ile Lys Thr Lys Pro Asn Ile Tyr Ala Thr
                  50                  55                  60

 Glu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
                  65                  70                  75

 Asp Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala
                  80                  85                  90

 Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Leu Thr Gly Met Lys
                  95                 100                 105

 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr
                 110                

<210> 13
<211> 114
<212> PRT
<213> Humanized

<400> 13
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser
                  20                  25                  30

 Ala Arg Trp Ile Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Gly Ile Lys Thr Lys Pro Ala Ile Tyr Ala Thr
                  50                  55                  60

 Glu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
                  65                  70                  75

 Asp Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala
                  80                  85                  90

 Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Leu Thr Gly Met Lys
                  95                 100                 105

 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr
                 110                

<210> 14
<211> 114
<212> PRT
<213> Humanized

<400> 14
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser
                  20                  25                  30

 Ala Arg Trp Ile Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Gly Ile Lys Thr Lys Pro Ala Ile Tyr Ala Thr
                  50                  55                  60

 Glu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
                  65                  70                  75

 Asp Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala
                  80                  85                  90

 Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Leu Thr Gly Met Lys
                  95                 100                 105

 Tyr Phe Asp Tyr Trp Gly Gln Gly Thr
                 110                

<210> 15
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 3
<223> X is S or T

<220>
<221> Variant
<222> 4
<223> X is S, P, or T

<220>
<221> Variant
<222> 5
<223> X is Q or N

<220>
<221> Variant
<222> 7
<223> X is F or S

<220>
<221> Variant
<222> 8
<223> X is T or I

<220>
<221> Variant
<222> 10
<223> X is K or T

<220>
<221> Variant
<222> 12
<223> X is G, D, or S

<400> 15
 Thr Leu Xaa Xaa Xaa His Xaa Xaa Tyr Xaa Ile Xaa
                   5                  10        

<210> 16
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 1
<223> X is L or I

<220>
<221> Variant
<222> 2
<223> X is N or T

<220>
<221> Variant
<222> 4
<223> X is D, H, or S

<220>
<221> Variant
<222> 5
<223> X is G or S

<220>
<221> Variant
<222> 6
<223> X is S, L, or T

<220>
<221> Variant
<222> 7
<223> X is H or Y

<220>
<221> Variant
<222> 8
<223> X is N, K, Q or I

<220>
<221> Variant
<222> 9
<223> X is K or T

<220>
<221> Variant
<222> 10
<223> X is G or A

<220>
<221> Variant
<222> 11
<223> X is D, S, or V

<400> 16
 Xaa Xaa Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
                   5                  10    

<210> 17
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 1
<223> X is G or A

<220>
<221> Variant
<222> 2
<223> X is S, A, or Y

<220>
<221> Variant
<222> 3
<223> X is S or Y

<220>
<221> Variant
<222> 5
<223> X is S, A, or Y

<220>
<221> Variant
<222> 6
<223> X is S, Y, or T

<220>
<221> Variant
<222> 9
<223> X is V or I

<400> 17
 Xaa Xaa Xaa Tyr Xaa Xaa Gly Tyr Xaa
                   5                

<210> 18
<211> 10
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 6
<223> X is N or A

<220>
<221> Variant
<222> 9
<223> X is I or V

<400> 18
 Gly Phe Thr Phe Ser Xaa Arg Trp Xaa Tyr
                   5                  10

<210> 19
<211> 19
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 7
<223> X is N, A, or T

<220>
<221> Variant
<222> 8
<223> X is I or R

<220>
<221> Variant
<222> 12
<223> X is E or Q

<400> 19
 Gly Ile Lys Thr Lys Pro Xaa Xaa Tyr Ala Thr Xaa Tyr Ala Asp
   1               5                  10                  15

 Ser Val Lys Gly
                

<210> 20
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<220>
<221> Variant
<222> 1
<223> X is L or V

<220>
<221> Variant
<222> 4
<223> X is M or K

<220>
<221> Variant
<222> 5
<223> X is R or K

<400> 20
 Xaa Thr Gly Xaa Xaa Tyr Phe Asp Tyr
                   5                

<210> 21
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 21
 Thr Leu Ser Ser Gln His Ser Thr Tyr Thr Ile Gly
                   5                  10        

<210> 22
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 22
 Thr Leu Ser Pro Gln His Phe Thr Tyr Lys Ile Asp
                   5                  10        

<210> 23
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 23
 Thr Leu Ser Ser Asn His Ser Ile Tyr Thr Ile Ser
                   5                  10        

<210> 24
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 24
 Thr Leu Thr Thr Gln His Ser Thr Tyr Thr Ile Gly
                   5                  10        

<210> 25
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 25
 Leu Asn Ser Asp Gly Ser His Asn Lys Gly Asp
                   5                  10    

<210> 26
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 26
 Ile Asn Ser Asp Gly Ser His Lys Lys Gly Val
                   5                  10    

<210> 27
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 27
 Leu Thr Ser His Gly Leu His Gln Lys Gly Val
                   5                  10    

<210> 28
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 28
 Leu Asn Ser Asp Ser Thr Tyr Ile Thr Ala Val
                   5                  10    

<210> 29
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 29
 Gly Ser Ser Tyr Ser Ser Gly Tyr Val
                   5                

<210> 30
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 30
 Ala Ala Tyr Tyr Ala Tyr Gly Tyr Val
                   5                

<210> 31
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 31
 Gly Tyr Ser Tyr Tyr Ser Gly Tyr Val
                   5                

<210> 32
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 32
 Gly Ser Ser Tyr Ser Thr Gly Tyr Val
                   5                

<210> 33
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 33
 Gly Ala Ser Tyr Ser Ser Gly Tyr Ile
                   5                

<210> 34
<211> 10
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 34
 Gly Phe Thr Phe Ser Asn Arg Trp Ile Tyr
                   5                  10

<210> 35
<211> 10
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 35
 Gly Phe Thr Phe Ser Asn Arg Trp Val Tyr
                   5                  10

<210> 36
<211> 19
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 36
 Gly Ile Lys Thr Lys Pro Asn Ile Tyr Ala Thr Glu Tyr Ala Asp
   1               5                  10                  15

 Ser Val Lys Gly
                

<210> 37
<211> 19
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 37
 Gly Ile Lys Thr Lys Pro Thr Arg Tyr Ala Thr Gln Tyr Ala Asp
   1               5                  10                  15

 Ser Val Lys Gly
                

<210> 38
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 38
 Leu Thr Gly Met Arg Tyr Phe Asp Tyr
                   5                

<210> 39
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 39
 Leu Thr Gly Met Lys Tyr Phe Asp Tyr
                   5                

<210> 40
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 40
 Val Thr Gly Met Arg Tyr Phe Asp Tyr
                   5                

