
                                SEQUENCE LISTING

<110> Herring, Christopher
      Holt, Lucy
      Jespers, Laurent
      Mayer, Sebastian
      Pupecka, Malgorzata
      
      
      

<120> Drug fusions and conjugates
  

<130> DB63558 WO

<150> US 61/163917        
<151> 2009-03-27  

<160> 52

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 168
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2xGLP-1 A8G DOM7h-14 fusion (DAT0114)

<400> 1
His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly
 1               5                  10                  15      
Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg His Gly
            20                  25                  30          
Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala
        35                  40                  45              
Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Asp Ile Gln Met
    50                  55                  60                  
Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr
65                  70                  75                  80  
Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr
                85                  90                  95      
Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser
            100                 105                 110         
Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly
        115                 120                 125             
Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala
    130                 135                 140                 
Thr Tyr Tyr Cys Ala Gln Gly Ala Ala Leu Pro Arg Thr Phe Gly Gln
145                 150                 155                 160 
Gly Thr Lys Val Glu Ile Lys Arg
                165             


<210> 2
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exendin 4, (G4S)3 linker, DOM7h-14 fusion
      (DAT0115)

<400> 2
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly
        35                  40                  45              
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser
    50                  55                  60                  
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala
65                  70                  75                  80  
Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly
                85                  90                  95      
Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser Ser Leu Gln Ser Gly
            100                 105                 110         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
        115                 120                 125             
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala
    130                 135                 140                 
Gln Gly Ala Ala Leu Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu
145                 150                 155                 160 
Ile Lys Arg
            


<210> 3
<211> 148
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exendin 4 DOM7h-14 fusion (DAT0116)

<400> 3
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Asp Ile Gln Met Thr Gln Ser Pro
        35                  40                  45              
Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg
    50                  55                  60                  
Ala Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro
65                  70                  75                  80  
Gly Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser Ser Leu Gln Ser
                85                  90                  95      
Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr
            100                 105                 110         
Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys
        115                 120                 125             
Ala Gln Gly Ala Ala Leu Pro Arg Thr Phe Gly Gln Gly Thr Lys Val
    130                 135                 140                 
Glu Ile Lys Arg
145             


<210> 4
<211> 188
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exendin 4, helical linker, DOM7h-14 fusion
      (DAT0117)

<400> 4
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Lys Glu Ala Ala Ala Lys Glu Ala
        35                  40                  45              
Ala Ala Lys Glu Ala Ala Ala Lys Glu Leu Ala Ala Lys Glu Ala Ala
    50                  55                  60                  
Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Leu Ala Ala
65                  70                  75                  80  
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
                85                  90                  95      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln
            100                 105                 110         
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        115                 120                 125             
Met Trp Arg Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    130                 135                 140                 
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
145                 150                 155                 160 
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Gly Ala Ala Leu Pro Arg
                165                 170                 175     
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            180                 185             


<210> 5
<211> 153
<212> PRT
<213> Artificial Sequence

<220> 
<223> GLP-1 A8G, (G4S)3, linker DOM7h-14 fusion
      (DAT0118)

<400> 5
His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly
 1               5                  10                  15      
Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly Gly
            20                  25                  30          
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln
        35                  40                  45              
Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val
    50                  55                  60                  
Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp
65                  70                  75                  80  
Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Met Trp Arg
                85                  90                  95      
Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser
            100                 105                 110         
Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe
        115                 120                 125             
Ala Thr Tyr Tyr Cys Ala Gln Gly Ala Ala Leu Pro Arg Thr Phe Gly
    130                 135                 140                 
Gln Gly Thr Lys Val Glu Ile Lys Arg
145                 150             


<210> 6
<211> 142
<212> PRT
<213> Artificial Sequence

<220> 
<223> GLP-1 A8G, PSS linker, DOM7h-14 fusion (DAT0119)

<400> 6
His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly
 1               5                  10                  15      
Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly Pro
            20                  25                  30          
Ser Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser
        35                  40                  45              
Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly
    50                  55                  60                  
Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
65                  70                  75                  80  
Leu Ile Met Trp Arg Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe
                85                  90                  95      
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu
            100                 105                 110         
Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Gly Ala Ala Leu
        115                 120                 125             
Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
    130                 135                 140         


<210> 7
<211> 179
<212> PRT
<213> Artificial Sequence

<220> 
<223> GLP-1 A8G, helical linker, DOM7h-14 fusion
      (DAT0120)

<400> 7
His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly
 1               5                  10                  15      
Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly Lys
            20                  25                  30          
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu
        35                  40                  45              
Leu Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala
    50                  55                  60                  
Ala Ala Lys Glu Leu Ala Ala Asp Ile Gln Met Thr Gln Ser Pro Ser
65                  70                  75                  80  
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala
                85                  90                  95      
Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly
            100                 105                 110         
Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser Ser Leu Gln Ser Gly
        115                 120                 125             
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
    130                 135                 140                 
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala
145                 150                 155                 160 
Gln Gly Ala Ala Leu Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu
                165                 170                 175     
Ile Lys Arg
            


<210> 8
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> DOM7h-14

<400> 8
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln
            20                  25                  30          
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Met Trp Arg Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Gly Ala Ala Leu Pro Arg
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105             


<210> 9
<211> 31
<212> PRT
<213> Artificial Sequence

<220> 
<223> GLP-1 (7-37) A8G

<400> 9
His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly
 1               5                  10                  15      
Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly
            20                  25                  30      


<210> 10
<211> 39
<212> PRT
<213> Artificial Sequence

<220> 
<223> exendin-4

<400> 10
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser
        35                  


<210> 11
<211> 40
<212> PRT
<213> Artificial Sequence

<220> 
<223> helical linker

<400> 11
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys
 1               5                  10                  15      
Glu Leu Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu
            20                  25                  30          
Ala Ala Ala Lys Glu Leu Ala Ala
        35                  40  


<210> 12
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Gly-ser linker

<400> 12
Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15      


<210> 13
<211> 504
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0114 - nucleic acid sequence (from mammalian
      construct)

<400> 13
catggtgaag ggacctttac cagtgatgta agttcttatt tggaaggcca agctgccaag 60
gaattcattg cttggctggt gaaaggccga catggtgaag ggacctttac cagtgatgta 120
agttcttatt tggaaggcca agctgccaag gaattcattg cttggctggt gaaaggccga 180
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 240
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 300
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 360
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 420
gaagattttg ctacgtacta ctgtgctcag ggtgcggcgt tgcctaggac gttcggccaa 480
gggaccaagg tggaaatcaa acgg                                        504

<210> 14
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0115 - nucleic acid sequence (from mammalian
      construct)

<400> 14
catggtgaag gaacatttac cagtgacttg tcaaaacaga tggaagagga ggcagtgcgg 60
ttatttattg agtggcttaa gaacggagga ccaagtagcg gggcacctcc gccatcgggt 120
ggtggaggcg gttcaggcgg aggtggcagc ggcggtggcg ggtcggacat ccagatgacc 180
cagtctccat cctccctgtc tgcatctgta ggagaccgtg tcaccatcac ttgccgggca 240
agtcagtgga ttgggtctca gttatcttgg taccagcaga aaccagggaa agcccctaag 300
ctcctgatca tgtggcgttc ctcgttgcaa agtggggtcc catcacgttt cagtggcagt 360
ggatctggga cagatttcac tctcaccatc agcagtctgc aacctgaaga ttttgctacg 420
tactactgtg ctcagggtgc ggcgttgcct aggacgttcg gccaagggac caaggtggaa 480
atcaaacgg                                                         489

<210> 15
<211> 495
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0115 - nucleic acid sequence (from E.coli
      construct)

<400> 15
cacggtgaag gtaccttcac ctctgacctg agcaaacaga tggaggaaga agcggttcgt 60
ctgttcatcg agtggctgaa aaacggtggt ccgtcttctg gtgctccgcc accgtctggt 120
ggtggtggtg gttctggtgg tggtggttct ggtggtggcg gtagcgacat ccagatgact 180
cagtccccaa gctctctgtc tgcctccgtt ggcgatcgtg ttacgatcac gtgccgtgct 240
tctcagtgga tcggttccca gctgtcctgg tatcagcaga aaccgggcaa agccccgaaa 300
ctcctgatca tgtggcgtag ctctctgcag tctggtgtac cgagccgctt ctctggttct 360
ggttctggta ccgacttcac cctgaccatt tcctctctgc agccggaaga tttcgcgacc 420
tactactgtg ctcagggtgc ggcactgcca cgtacttttg gccagggtac gaaagtcgag 480
attaaacgtt aatga                                                  495

<210> 16
<211> 444
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0116 - nucleic acid sequence (from mammalian
      construct)

<400> 16
catggtgaag gaacatttac cagtgacttg tcaaaacaga tggaagagga ggcagtgcgg 60
ttatttattg agtggcttaa gaacggagga ccaagtagcg gggcacctcc gccatcgggt 120
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 180
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 240
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 300
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 360
gaagattttg ctacgtacta ctgtgctcag ggtgcggcgt tgcctaggac gttcggccaa 420
gggaccaagg tggaaatcaa acgg                                        444

<210> 17
<211> 447
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0116 - nucleic acid sequence (from E.coli
      construct)

<400> 17
cacggtgaag gtaccttcac ctctgacctg agcaaacaga tggaggaaga agcggttcgt 60
ctgttcatcg agtggctgaa aaacggtggt ccgtcttctg gtgctccgcc accgtctgac 120
atccagatga ctcagtcccc aagctctctg tctgcctccg ttggcgatcg tgttacgatc 180
acgtgccgtg cttctcagtg gatcggttcc cagctgtcct ggtatcagca gaaaccgggc 240
aaagccccga aactcctgat catgtggcgt agctctctgc agtctggtgt accgagccgc 300
ttctctggtt ctggttctgg taccgacttc accctgacca tttcctctct gcagccggaa 360
gatttcgcga cctactactg tgctcagggt gcggcactgc cacgtacttt tggccagggt 420
acgaaagtcg agattaaacg ttaatga                                     447

<210> 18
<211> 564
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0117 - nucleic acid sequence (from mammalian
      construct)

<400> 18
catggtgaag gaacatttac cagtgacttg tcaaaacaga tggaagagga ggcagtgcgg 60
ttatttattg agtggcttaa gaacggagga ccaagtagcg gggcacctcc gccatcgggt 120
aaagaagcgg cggcgaaaga agcggcggcg aaagaagcgg cggcgaaaga attggccgca 180
aaagaagcgg cggcgaaaga agcggcggcg aaagaagcgg cggcgaaaga attggccgca 240
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 300
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 360
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 420
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 480
gaagattttg ctacgtacta ctgtgctcag ggtgcggcgt tgcctaggac gttcggccaa 540
gggaccaagg tggaaatcaa acgg                                        564

<210> 19
<211> 567
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0117 - nucleic acid sequence (from E.coli
      construct)

<400> 19
cacggtgaag gtaccttcac ctctgacctg agcaaacaga tggaggaaga agcggttcgt 60
ctgttcatcg agtggctgaa aaacggtggt ccgtcttctg gtgctccgcc accgtctaaa 120
gaagcggcgg cgaaagaagc ggcggcgaaa gaagcggcgg cgaaagaatt ggccgcaaaa 180
gaagcggcgg cgaaagaagc ggcggcgaaa gaagcggcgg cgaaagaatt ggccgcagac 240
atccagatga ctcagtcccc aagctctctg tctgcctccg ttggcgatcg tgttacgatc 300
acgtgccgtg cttctcagtg gatcggttcc cagctgtcct ggtatcagca gaaaccgggc 360
aaagccccga aactcctgat catgtggcgt agctctctgc agtctggtgt accgagccgc 420
ttctctggtt ctggttctgg taccgacttc accctgacca tttcctctct gcagccggaa 480
gatttcgcga cctactactg tgctcagggt gcggcactgc cacgtacttt tggccagggt 540
acgaaagtcg agattaaacg ttaatga                                     567

<210> 20
<211> 459
<212> DNA
<213> DAT0118 - nucleic acid sequence (from mammalian c)

<220> 
<223> DAT0118 - nucleic acid sequence (from mammalian
      construct)

<400> 20
catggtgaag ggacctttac cagtgatgta agttcttatt tggaaggcca agctgccaag 60
gaattcattg cttggctggt gaaaggccga ggtggaggcg gttcaggcgg aggtggcagc 120
ggcggtggcg ggtcggacat ccagatgacc cagtctccat cctccctgtc tgcatctgta 180
ggagaccgtg tcaccatcac ttgccgggca agtcagtgga ttgggtctca gttatcttgg 240
taccagcaga aaccagggaa agcccctaag ctcctgatca tgtggcgttc ctcgttgcaa 300
agtggggtcc catcacgttt cagtggcagt ggatctggga cagatttcac tctcaccatc 360
agcagtctgc aacctgaaga ttttgctacg tactactgtg ctcagggtgc ggcgttgcct 420
aggacgttcg gccaagggac caaggtggaa atcaaacgg                        459

<210> 21
<211> 426
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0119 - nucleic acid sequence (from mammalian
      construct)

<400> 21
catggtgaag ggacctttac cagtgatgta agttcttatt tggaaggcca agctgccaag 60
gaattcattg cttggctggt gaaaggccga ggaccaagct cggacatcca gatgacccag 120
tctccatcct ccctgtctgc atctgtagga gaccgtgtca ccatcacttg ccgggcaagt 180
cagtggattg ggtctcagtt atcttggtac cagcagaaac cagggaaagc ccctaagctc 240
ctgatcatgt ggcgttcctc gttgcaaagt ggggtcccat cacgtttcag tggcagtgga 300
tctgggacag atttcactct caccatcagc agtctgcaac ctgaagattt tgctacgtac 360
tactgtgctc agggtgcggc gttgcctagg acgttcggcc aagggaccaa ggtggaaatc 420
aaacgg                                                            426

<210> 22
<211> 537
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAT0120 - nucleic acid sequence (from mammalian
      construct)

<400> 22
catggtgaag ggacctttac cagtgatgta agttcttatt tggaaggcca agctgccaag 60
gaattcattg cttggctggt gaaaggccga ggaaaagaag cggcggcgaa agaagcggcg 120
gcgaaagaag cggcggcgaa agaattggcc gcaaaagaag cggcggcgaa agaagcggcg 180
gcgaaagaag cggcggcgaa agaattggcc gcagacatcc agatgaccca gtctccatcc 240
tccctgtctg catctgtagg agaccgtgtc accatcactt gccgggcaag tcagtggatt 300
gggtctcagt tatcttggta ccagcagaaa ccagggaaag cccctaagct cctgatcatg 360
tggcgttcct cgttgcaaag tggggtccca tcacgtttca gtggcagtgg atctgggaca 420
gatttcactc tcaccatcag cagtctgcaa cctgaagatt ttgctacgta ctactgtgct 480
cagggtgcgg cgttgcctag gacgttcggc caagggacca aggtggaaat caaacgg    537

<210> 23
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> Dom7h-14 - nucleic acid sequence

<400> 23
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 60
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 120
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 180
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg ctacgtacta ctgtgctcag ggtgcggcgt tgcctaggac gttcggccaa 300
gggaccaagg tggaaatcaa acgg                                        324

<210> 24
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exendin 4, (G4S)3, linker DOM7h-14-10 fusion
      (DMS7139)

<400> 24
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly
        35                  40                  45              
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser
    50                  55                  60                  
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala
65                  70                  75                  80  
Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly
                85                  90                  95      
Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser Ser Leu Gln Ser Gly
            100                 105                 110         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
        115                 120                 125             
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala
    130                 135                 140                 
Gln Gly Leu Arg His Pro Lys Thr Phe Gly Gln Gly Thr Lys Val Glu
145                 150                 155                 160 
Ile Lys Arg
            


<210> 25
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exendin 4, (G4S)3, linker DOM7h-11-15 fusion
      (DMS7143)

<400> 25
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly
        35                  40                  45              
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser
    50                  55                  60                  
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala
65                  70                  75                  80  
Ser Arg Pro Ile Gly Thr Met Leu Ser Trp Tyr Gln Gln Lys Pro Gly
                85                  90                  95      
Lys Ala Pro Lys Leu Leu Ile Leu Ala Phe Ser Arg Leu Gln Ser Gly
            100                 105                 110         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
        115                 120                 125             
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala
    130                 135                 140                 
Gln Ala Gly Thr His Pro Thr Thr Phe Gly Gln Gly Thr Lys Val Glu
145                 150                 155                 160 
Ile Lys Arg
            


<210> 26
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> DOM7h-14-10

<400> 26
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln
            20                  25                  30          
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Met Trp Arg Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Gly Leu Arg His Pro Lys
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105             


<210> 27
<211> 108
<212> PRT
<213> DOM7h-11-15Artificial Sequence

<220> 
<223> DOM7h-11-15

<400> 27
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Arg Pro Ile Gly Thr Met
            20                  25                  30          
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Leu Ala Phe Ser Arg Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Ala Gly Thr His Pro Thr
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105             


<210> 28
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> OmpT AWA signal peptide (leader)

<400> 28
Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile
 1               5                  10                  15      
Ser Ala Trp Ala
            20  


<210> 29
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> Exendin 4, (G4S)3, linker DOM7h-14-10 fusion
      (DMS7139)

<400> 29
catggtgaag gaacatttac cagtgacttg tcaaaacaga tggaagagga ggcagtgcgg 60
ttatttattg agtggcttaa gaacggagga ccaagtagcg gggcacctcc gccatcgggt 120
ggtggaggcg gttcaggcgg aggtggcagc ggcggtggcg ggtcggacat ccagatgacc 180
cagtctccat cctccctgtc tgcatctgta ggagaccgtg tcaccatcac ttgccgggca 240
agtcagtgga ttgggtctca gttatcttgg taccagcaga aaccagggaa agcccctaag 300
ctcctgatca tgtggcgttc ctcgttgcaa agtggggtcc catcacgttt cagtggcagt 360
ggatctggga cagatttcac tctcaccatc agcagtctgc aacctgaaga ttttgctacg 420
tactactgtg ctcagggttt gaggcatcct aagacgttcg gccaagggac caaggtggaa 480
atcaaacgg                                                         489

<210> 30
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> Exendin 4, (G4S)3, linker DOM7h-11-115 fusion
      (DMS7143)

<400> 30
catggtgaag gaacatttac cagtgacttg tcaaaacaga tggaagagga ggcagtgcgg 60
ttatttattg agtggcttaa gaacggagga ccaagtagcg gggcacctcc gccatcgggt 120
ggtggaggcg gttcaggcgg aggtggcagc ggcggtggcg ggtcggacat ccagatgacc 180
cagtctccat cctccctgtc tgcatctgta ggagaccgtg tcaccatcac ttgccgggca 240
agtcgtccga ttgggacgat gttaagttgg taccagcaga aaccagggaa agcccctaag 300
ctcctgatcc ttgctttttc ccgtttgcaa agtggggtcc catcacgttt cagtggcagt 360
ggatctggga cagatttcac tctcaccatc agcagtctgc aacctgaaga ttttgctacg 420
tactactgcg cgcaggctgg gacgcatcct acgacgttcg gccaagggac caaggtggaa 480
atcaaacgg                                                         489

<210> 31
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> Dom7h-14-10  - nucleic acid

<400> 31
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 60
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 120
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 180
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg ctacgtacta ctgtgctcag ggtttgaggc atcctaagac gttcggccaa 300
gggaccaagg tggaaatcaa acgg                                        324

<210> 32
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> Dom7h-11-15  - nucleic acid

<400> 32
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 60
atcacttgcc gggcaagtcg tccgattggg acgatgttaa gttggtacca gcagaaacca 120
gggaaagccc ctaagctcct gatccttgct ttttcccgtt tgcaaagtgg ggtcccatca 180
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg ctacgtacta ctgcgcgcag gctgggacgc atcctacgac gttcggccaa 300
gggaccaagg tggaaatcaa acgg                                        324

<210> 33
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> OmpAWA signal peptide  - nucleic acid sequence

<400> 33
atgcgggcga aactcctagg aatagtcctg acaaccccta tcgcgatcag cgcttgggcc 60


<210> 34
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> ompA  (E. coli derived)

<400> 34
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Gln Ala
            20      


<210> 35
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> ompA-AMA  (artificial sequence)

<400> 35
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Met Ala
            20      


<210> 36
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> ompA-AWA

<400> 36
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Trp Ala
            20      


<210> 37
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> ompT  (E. coli derived)

<400> 37
Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile
 1               5                  10                  15      
Ser Ser Phe Ala
            20  


<210> 38
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> ompT-AMA

<400> 38
Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile
 1               5                  10                  15      
Ser Ala Met Ala
            20  


<210> 39
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> GAS  (S. cerevisiae derived)

<400> 39
Met Leu Phe Lys Ser Leu Ser Lys Leu Ala Thr Ala Ala Ala Phe Phe
 1               5                  10                  15      
Ala Gly Val Ala Thr Ala
            20          


<210> 40
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> GAS-AMA

<400> 40
Met Leu Phe Lys Ser Leu Ser Lys Leu Ala Thr Ala Ala Ala Phe Phe
 1               5                  10                  15      
Ala Gly Val Ala Met Ala
            20          


<210> 41
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> GAS-AWA

<400> 41
Met Leu Phe Lys Ser Leu Ser Lys Leu Ala Thr Ala Ala Ala Phe Phe
 1               5                  10                  15      
Ala Gly Val Ala Trp Ala
            20          


<210> 42

<220> 
<223> DOM7h-14-10R108C

<400> 42
 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Trp Ile Gly Ser Gln
            20                  25                  30          
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Met Trp Arg Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Gly Leu Arg His Pro Lys
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Cys
            100                 105             


<210> 43
<211> 34
<212> PRT
<213> Artificial Sequence

<220> 
<223> PYY 3-36 (with a lysine at position 10)

<400> 43
Ile Lys Pro Glu Ala Pro Gly Lys Asp Ala Ser Pro Glu Glu Leu Asn
 1               5                  10                  15      
Arg Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Leu Val Thr Arg Gln
            20                  25                  30          
Arg Tyr
        


<210> 44
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> DOM 7h-14-10 R108C  - nucleic acid sequence

<400> 44
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga ccgtgtcacc 60
atcacttgcc gggcaagtca gtggattggg tctcagttat cttggtacca gcagaaacca 120
gggaaagccc ctaagctcct gatcatgtgg cgttcctcgt tgcaaagtgg ggtcccatca 180
cgtttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg ctacgtacta ctgtgctcag ggtttgaggc atcctaagac gttcggccaa 300
gggaccaagg tggaaatcaa atgt                                        324

<210> 45
<211> 1389
<212> DNA
<213> Artificial Sequence

<220> 
<223> Cynomolgus GLP-1 receptor  cDNA sequence

<400> 45
atggccggca cccccggccc gctgcgcctc gcgctcctgc tgctcggggt ggtgggcagg 60
gccggccccc gcccccaggg tgccactgtg tccctctggg agacggtgca gaaatggcga 120
gaataccgac gccagtgcca gcgctccctg actgaggacc cacctcccgc cacagacttg 180
ttctgcaacc ggaccttcga tgaatatgcc tgctggccag atggggagcc aggctccttc 240
gtgaatgtca gctgcccctg gtacctgccc tgggccagca gtgtgccgca gggccacgtg 300
taccggttct gcacagctga aggcctctgg ctgcagaagg acaactccag cctgccctgg 360
agggacttgt cggagtgtga ggagtccaag cgaggggaga gaaattcccc ggaggagcag 420
ctcctgtccc tctacatcat ctacacggtg ggctacgcac tctccttctc tgctctggtt 480
atcgcctctg cgatcctcct tggcttcaga cacctgcact gcacccggaa ctacatccac 540
ctgaacctgt ttgcatcctt catcctgcga gcattgtccg tcttcatcaa ggacgcagcc 600
ctcaagtgga tgtacagcac ggccgcccag cagcaccagt gggatgggct cctctcctac 660
caggactctc tgggctgccg cgtggtgttt ctgctcatgc aatactgtgt ggcggccaat 720
tactactggc tcttggtgga gggcgtgtac ctgtacacac tgctggcctt ctcggtcttc 780
tctgagcaac gaatcttcag gctgtatgtg agcgtaggct ggggtgttcc cctgctgttt 840
gttgtcccct ggggcattgt caagtacctc tatgaggacg agggctgctg gaccaggaac 900
tccaacatga actactggct cattatccgg ctgcccattc tctttgccat tggggtgaac 960
ttcctcatct ttgttcgggt catctgcatc gtggtatcca aactgaaggc caatctcatg 1020
tgcaagacag acatcaaatg cagacttgcc aagtccacgc tgacactcat ccccctgctg 1080
gggactcatg aggtcatctt tgcctttgtg atggacgagc atgcccgggg caccctgcgc 1140
ttcatcaagc tgttcacgga gctctccttt acctccttcc aggggctgat ggtggccatc 1200
ttgtactgct ttgtcaacaa tgaggtccag ttggaatttc ggaagagctg ggagcgctgg 1260
cggcttgagc acttgcacat ccagagggac agcagcatga agcccctcaa gtgtcccacc 1320
agcagcctga gcagtggggc cacggcgggc agcagcatgt acacagccac ttgccaggcc 1380
tcctgcagc                                                         1389

<210> 46
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> Pel B

<400> 46
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala
 1               5                  10                  15      
Ala Gln Pro Ala Met Ala
            20          


<210> 47
<211> 115
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide 1

<400> 47
ggaattccat atgaaaatca aaaccggtgc tcgcatcctg gctctgtccg ctctgaccac 60
tatgatgttc tccgcttccg cgctggctca tggtgaagga acatttacca gtgac      115

<210> 48
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotide 2

<400> 48
gttcagaatt cttattaccg tttgatttcc accttggtcc cttg                  44

<210> 49
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mal E signal sequence

<400> 49
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr
 1               5                  10                  15      
Thr Met Met Phe Ser Ala Ser Ala Leu Ala
            20                  25      


<210> 50
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> DMS 7139 nucleic acid sequence for E.coli
      expression

<400> 50
cacggtgaag gtacgttcac ctctgacctg agcaaacaga tggaggaaga agcggttcgt 60
ctgttcatcg agtggctgaa aaacggtggt ccgtcttctg gtgctccgcc gccgtctggt 120
ggtggtggtg gttctggtgg tggtggttct ggtggtggtg gcagcgatat ccagatgact 180
cagtccccgt cttctctctc cgcctctgtt ggcgaccgtg ttaccatcac ttgtcgtgcg 240
agccagtgga tcggttccca gctgagctgg tatcagcaga aaccgggcaa agcgccgaaa 300
ctgctgatca tgtggcgctc tagcctgcag tctggtgtac cgtctcgttt ctccggctct 360
ggttctggta cggacttcac cctcacgatc tcttccctgc agccggaaga ctttgccacc 420
tactactgcg cacagggtct gcgtcacccg aaaaccttcg gtcagggtac caaagtcgag 480
atcaaacgt                                                         489

