
                                SEQUENCE LISTING

<110> aTyr Pharma Inc.
      Zhou, Jie
      Fun, Lau Ching
      Xu, Zhiwen
      Lo, Wing Sze
      Piehl, Kristi Helen
      Greene, Leslie Ann

<120> COMPOSITIONS AND METHODS COMPRISING
  HISTIDYL-tRNA SYNTHETASE SPLICE VARIANTS HAVING
  NON-CANONICAL BIOLOGICAL ACTIVITIES

<130> 120161.415PC

<140> PCT                  
<141> 2010-03-16  

<160> 29

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 1
agtggacagc cgggatggca gagc                                        24

<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 2
caggaagtcg cctatctgaa g                                           21

<210> 3
<211> 1981
<212> DNA
<213> Homo sapiens

<400> 3
tcgatagccg gaagtcatcc ttgctgaggc tggggcaacc accgcaggtc gagacagcag 60
gcggctcaag tggacagccg ggatggcaga gcgtgcggcg ctggaggagc tggtgaaact 120
tcagggagag cgcgtgcgag gcctcaagca gcagaaggcc agcgccgagc tgatcgagga 180
ggaggtggcg aaactcctga aactgaaggc acagctgggt cctgatgaaa gcaaacagaa 240
atttgtgctc aaaaccccca agggcacaag agactatagt ccccggcaga tggcagttcg 300
cgagaaggtg tttgacgtaa tcatccgttg cttcaagcgc cacggtgcag aagtcattga 360
tacacctgta tttgaactaa aggaaacact gatgggaaag tatggggaag actccaagct 420
tatctatgac ctgaaggacc agggcgggga gctcctgtcc cttcgctatg acctcactgt 480
tccttttgct cggtatttgg caatgaataa actgaccaac attaaacgct accacatagc 540
aaaggtatat cggcgggata acccagccat gacccgtggc cgataccggg aattctacca 600
gtgtgatttt gacattgctg ggaactttga tcccatgatc cctgatgcag agtgcctgaa 660
gatcatgtgc gagatcctga gttcacttca gataggcgac ttcctggtca aggtaaacga 720
tcgacgcatt ctagatggga tgtttgctat ctgtggtgtt tctgacagca agttccgtac 780
catctgctcc tcagtagaca agctggacaa ggtgtcctgg gaagaggtga agaatgagat 840
ggtgggagag aagggccttg cacctgaggt ggctgaccgc attggggact atgtccagca 900
acatggtggg gtatccctgg tggaacagct gctccaggat cctaaactat cccaaaacaa 960
gcaggccttg gagggcctgg gagacctgaa gttgctcttt gagtacctga ccctatttgg 1020
cattgatgac aaaatctcct ttgacctgag ccttgctcga gggctggatt actacactgg 1080
ggtgatctat gaggcagtgc tgctacagac cccagcccag gcaggggaag agcccctggg 1140
tgtgggcagt gtggctgctg gaggacgcta tgatgggcta gtgggcatgt tcgaccccaa 1200
agggcgcaag gtgccatgtg tggggctcag cattggggtg gagcggattt tctccatcgt 1260
ggaacagaga ctagaggctt tggaggagaa gatacggacc acggagacac aggtgcttgt 1320
ggcatctgca cagaagaagc tgctagagga aagactaaag cttgtctcag aactgtggga 1380
tgctgggatc aaggctgagc tgctgtacaa gaagaaccca aagctactga accagttaca 1440
gtactgtgag gaggcaggca tcccactggt ggctatcatc ggcgagcagg aactcaagga 1500
tggggtcatc aagctccgtt cagtgacgag cagggaagag gtggatgtcc gaagagaaga 1560
ccttgtggag gaaatcaaaa ggagaacagg ccagcccctc tgcatctgct gaactgaaca 1620
aactatcaga ggaaaggaag tgggactggc actatttgag gttaagacaa actgcatatg 1680
tacttcaatt gctttgcact tttccgtttc agcggaagac ctgaagagtg gtcagaacag 1740
agcctttgat ttttattatg gttattttat tgattattac tggcaaaaac ggccaggtac 1800
aacacctttt tcatacaagg cccaggaggc ttagtccagt ctgtgctcct gggctacaag 1860
gacccagcct gagatggtcc catctgcagg gccccgcacc agttggagca gatgcctccc 1920
caccaccaat tgccaaaggt ccaataaaat gcctcaacca cggaaaaaaa aaaaaaaaaa 1980
a                                                                 1981

<210> 4
<211> 509
<212> PRT
<213> Homo sapiens

<400> 4
Met Ala Glu Arg Ala Ala Leu Glu Glu Leu Val Lys Leu Gln Gly Glu
 1               5                  10                  15      
Arg Val Arg Gly Leu Lys Gln Gln Lys Ala Ser Ala Glu Leu Ile Glu
            20                  25                  30          
Glu Glu Val Ala Lys Leu Leu Lys Leu Lys Ala Gln Leu Gly Pro Asp
        35                  40                  45              
Glu Ser Lys Gln Lys Phe Val Leu Lys Thr Pro Lys Gly Thr Arg Asp
    50                  55                  60                  
Tyr Ser Pro Arg Gln Met Ala Val Arg Glu Lys Val Phe Asp Val Ile
65                  70                  75                  80  
Ile Arg Cys Phe Lys Arg His Gly Ala Glu Val Ile Asp Thr Pro Val
                85                  90                  95      
Phe Glu Leu Lys Glu Thr Leu Met Gly Lys Tyr Gly Glu Asp Ser Lys
            100                 105                 110         
Leu Ile Tyr Asp Leu Lys Asp Gln Gly Gly Glu Leu Leu Ser Leu Arg
        115                 120                 125             
Tyr Asp Leu Thr Val Pro Phe Ala Arg Tyr Leu Ala Met Asn Lys Leu
    130                 135                 140                 
Thr Asn Ile Lys Arg Tyr His Ile Ala Lys Val Tyr Arg Arg Asp Asn
145                 150                 155                 160 
Pro Ala Met Thr Arg Gly Arg Tyr Arg Glu Phe Tyr Gln Cys Asp Phe
                165                 170                 175     
Asp Ile Ala Gly Asn Phe Asp Pro Met Ile Pro Asp Ala Glu Cys Leu
            180                 185                 190         
Lys Ile Met Cys Glu Ile Leu Ser Ser Leu Gln Ile Gly Asp Phe Leu
        195                 200                 205             
Val Lys Val Asn Asp Arg Arg Ile Leu Asp Gly Met Phe Ala Ile Cys
    210                 215                 220                 
Gly Val Ser Asp Ser Lys Phe Arg Thr Ile Cys Ser Ser Val Asp Lys
225                 230                 235                 240 
Leu Asp Lys Val Ser Trp Glu Glu Val Lys Asn Glu Met Val Gly Glu
                245                 250                 255     
Lys Gly Leu Ala Pro Glu Val Ala Asp Arg Ile Gly Asp Tyr Val Gln
            260                 265                 270         
Gln His Gly Gly Val Ser Leu Val Glu Gln Leu Leu Gln Asp Pro Lys
        275                 280                 285             
Leu Ser Gln Asn Lys Gln Ala Leu Glu Gly Leu Gly Asp Leu Lys Leu
    290                 295                 300                 
Leu Phe Glu Tyr Leu Thr Leu Phe Gly Ile Asp Asp Lys Ile Ser Phe
305                 310                 315                 320 
Asp Leu Ser Leu Ala Arg Gly Leu Asp Tyr Tyr Thr Gly Val Ile Tyr
                325                 330                 335     
Glu Ala Val Leu Leu Gln Thr Pro Ala Gln Ala Gly Glu Glu Pro Leu
            340                 345                 350         
Gly Val Gly Ser Val Ala Ala Gly Gly Arg Tyr Asp Gly Leu Val Gly
        355                 360                 365             
Met Phe Asp Pro Lys Gly Arg Lys Val Pro Cys Val Gly Leu Ser Ile
    370                 375                 380                 
Gly Val Glu Arg Ile Phe Ser Ile Val Glu Gln Arg Leu Glu Ala Leu
385                 390                 395                 400 
Glu Glu Lys Ile Arg Thr Thr Glu Thr Gln Val Leu Val Ala Ser Ala
                405                 410                 415     
Gln Lys Lys Leu Leu Glu Glu Arg Leu Lys Leu Val Ser Glu Leu Trp
            420                 425                 430         
Asp Ala Gly Ile Lys Ala Glu Leu Leu Tyr Lys Lys Asn Pro Lys Leu
        435                 440                 445             
Leu Asn Gln Leu Gln Tyr Cys Glu Glu Ala Gly Ile Pro Leu Val Ala
    450                 455                 460                 
Ile Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser
465                 470                 475                 480 
Val Thr Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu
                485                 490                 495     
Glu Ile Lys Arg Arg Thr Gly Gln Pro Leu Cys Ile Cys
            500                 505                 


<210> 5
<211> 183
<212> DNA
<213> Homo sapiens

<400> 5
atggcagagc gtgcggcgct ggaggagctg gtgaaacttc agggagagcg cgtgcgaggc 60
ctcaagcagc agaaggccag cgccgagctg atcgaggagg aggtggcgaa actcctgaaa 120
ctgaaggcac agctgggtcc tgatgaaagc aaacagaaat ttgtgctcaa aacccccaag 180
tag                                                               183

<210> 6
<211> 60
<212> PRT
<213> Homo sapiens

<400> 6
Met Ala Glu Arg Ala Ala Leu Glu Glu Leu Val Lys Leu Gln Gly Glu
 1               5                  10                  15      
Arg Val Arg Gly Leu Lys Gln Gln Lys Ala Ser Ala Glu Leu Ile Glu
            20                  25                  30          
Glu Glu Val Ala Lys Leu Leu Lys Leu Lys Ala Gln Leu Gly Pro Asp
        35                  40                  45              
Glu Ser Lys Gln Lys Phe Val Leu Lys Thr Pro Lys
    50                  55                  60  


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 7
atagtgccag tcccacttcc                                             20

<210> 8
<211> 516
<212> DNA
<213> Homo sapiens

<400> 8
atggcagagc gtgcggcgct ggaggagctg gtgaaacttc agggagagcg cgtgcgaggc 60
ctcaagcagc agaaggccag cgccgagctg atcgaggagg aggtggcgaa actcctgaaa 120
ctgaaggcac agctgggtcc tgatgaaagc aaacagaaat ttgtgctcaa aacccccaag 180
gctttggagg agaagatacg gaccacggag acacaggtgc ttgtggcatc tgcacagaag 240
aagctgctag aggaaagact aaagcttgtc tcagaactgt gggatgctgg gatcaaggct 300
gagctgctgt acaagaagaa cccaaagcta ctgaaccagt tacagtactg tgaggaggca 360
ggcatcccac tggtggctat catcggcgag caggaactca aggatggggt catcaagctc 420
cgttcagtga cgagcaggga agaggtggat gtccgaagag aagaccttgt ggaggaaatc 480
aaaaggagaa caggccagcc cctctgcatc tgctga                           516

<210> 9
<211> 171
<212> PRT
<213> Homo sapiens

<400> 9
Met Ala Glu Arg Ala Ala Leu Glu Glu Leu Val Lys Leu Gln Gly Glu
 1               5                  10                  15      
Arg Val Arg Gly Leu Lys Gln Gln Lys Ala Ser Ala Glu Leu Ile Glu
            20                  25                  30          
Glu Glu Val Ala Lys Leu Leu Lys Leu Lys Ala Gln Leu Gly Pro Asp
        35                  40                  45              
Glu Ser Lys Gln Lys Phe Val Leu Lys Thr Pro Lys Ala Leu Glu Glu
    50                  55                  60                  
Lys Ile Arg Thr Thr Glu Thr Gln Val Leu Val Ala Ser Ala Gln Lys
65                  70                  75                  80  
Lys Leu Leu Glu Glu Arg Leu Lys Leu Val Ser Glu Leu Trp Asp Ala
                85                  90                  95      
Gly Ile Lys Ala Glu Leu Leu Tyr Lys Lys Asn Pro Lys Leu Leu Asn
            100                 105                 110         
Gln Leu Gln Tyr Cys Glu Glu Ala Gly Ile Pro Leu Val Ala Ile Ile
        115                 120                 125             
Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser Val Thr
    130                 135                 140                 
Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu Glu Ile
145                 150                 155                 160 
Lys Arg Arg Thr Gly Gln Pro Leu Cys Ile Cys
                165                 170 

<210> 10
<211> 636
<212> DNA
<213> Homo sapiens

<400> 10
atggcagagc gtgcggcgct ggaggagctg gtgaaacttc agggagagcg cgtgcgaggc 60
ctcaagcagc agaaggccag cgccgagctg atcgaggagg aggtggcgaa actcctgaaa 120
ctgaaggcac agctgggtcc tgatgaaagc aaacagaaat ttgtgctcaa aacccccaag 180
ggcacaagag actatagtcc ccggcagatg gcagttcgcg agaaggtgtt tgacgtaatc 240
atccgttgct tcaagcgcca cggtgcagaa gtcattgata cacctgtatt tgaactaaag 300
gctttggagg agaagatacg gaccacggag acacaggtgc ttgtggcatc tgcacagaag 360
aagctgctag aggaaagact aaagcttgtc tcagaactgt gggatgctgg gatcaaggct 420
gagctgctgt acaagaagaa cccaaagcta ctgaaccagt tacagtactg tgaggaggca 480
ggcatcccac tggtggctat catcggcgag caggaactca aggatggggt catcaagctc 540
cgttcagtga cgagcaggga agaggtggat gtccgaagag aagaccttgt ggaggaaatc 600
aaaaggagaa caggccagcc cctctgcatc tgctga                           636

<210> 11
<211> 211
<212> PRT
<213> Homo sapiens

<400> 11
Met Ala Glu Arg Ala Ala Leu Glu Glu Leu Val Lys Leu Gln Gly Glu
 1               5                  10                  15      
Arg Val Arg Gly Leu Lys Gln Gln Lys Ala Ser Ala Glu Leu Ile Glu
            20                  25                  30          
Glu Glu Val Ala Lys Leu Leu Lys Leu Lys Ala Gln Leu Gly Pro Asp
        35                  40                  45              
Glu Ser Lys Gln Lys Phe Val Leu Lys Thr Pro Lys Gly Thr Arg Asp
    50                  55                  60                  
Tyr Ser Pro Arg Gln Met Ala Val Arg Glu Lys Val Phe Asp Val Ile
65                  70                  75                  80  
Ile Arg Cys Phe Lys Arg His Gly Ala Glu Val Ile Asp Thr Pro Val
                85                  90                  95      
Phe Glu Leu Lys Ala Leu Glu Glu Lys Ile Arg Thr Thr Glu Thr Gln
            100                 105                 110         
Val Leu Val Ala Ser Ala Gln Lys Lys Leu Leu Glu Glu Arg Leu Lys
        115                 120                 125             
Leu Val Ser Glu Leu Trp Asp Ala Gly Ile Lys Ala Glu Leu Leu Tyr
    130                 135                 140                 
Lys Lys Asn Pro Lys Leu Leu Asn Gln Leu Gln Tyr Cys Glu Glu Ala
145                 150                 155                 160 
Gly Ile Pro Leu Val Ala Ile Ile Gly Glu Gln Glu Leu Lys Asp Gly
                165                 170                 175     
Val Ile Lys Leu Arg Ser Val Thr Ser Arg Glu Glu Val Asp Val Arg
            180                 185                 190         
Arg Glu Asp Leu Val Glu Glu Ile Lys Arg Arg Thr Gly Gln Pro Leu
        195                 200                 205             
Cys Ile Cys
    210     


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence hsH1-E2F1

<400> 12
tgaaactgaa ggcacagctg                                             20

<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence hsH1-E13R1

<400> 13
tcttctcttc ggacatccac                                             20

<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence rnH1-E02F1

<400> 14
aacagaagtt cgtcctcaaa ac                                          22

<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence rnH1-E12J13R2

<400> 15
tccacctctt ctctgctcgt ca                                          22

<210> 16
<211> 60
<212> PRT
<213> Pongo abelii

<400> 16
Asn Gln Leu Gln Tyr Cys Glu Glu Ala Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Glu Asp Gly Val Ile Lys Leu Arg Ser Val
            20                  25                  30          
Thr Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu Glu
        35                  40                  45              
Ile Lys Arg Arg Thr Gly Gln Pro Leu Cys Ile Cys
    50                  55                  60  


<210> 17
<211> 60
<212> PRT
<213> Bos taurus

<400> 17
Asn Gln Leu Gln Tyr Cys Glu Glu Thr Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser Val
            20                  25                  30          
Ala Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu Glu
        35                  40                  45              
Ile Lys Arg Arg Thr Ser Gln Pro Leu Cys Ile Cys
    50                  55                  60  


<210> 18
<211> 60
<212> PRT
<213> Mus musculus

<400> 18
Asn Gln Leu Gln Tyr Cys Glu Glu Ala Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser Val
            20                  25                  30          
Ala Ser Arg Glu Glu Val Asp Val Gln Arg Glu Asp Leu Val Glu Glu
        35                  40                  45              
Ile Arg Arg Arg Thr Asn Gln Pro Leu Ser Ile Cys
    50                  55                  60  


<210> 19
<211> 60
<212> PRT
<213> Mesocricetus auratus

<400> 19
Asn Gln Leu Gln Tyr Cys Glu Glu Thr Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser Val
            20                  25                  30          
Ala Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu Glu
        35                  40                  45              
Ile Arg Arg Arg Thr Asn Gln Pro Leu Tyr Val Cys
    50                  55                  60  


<210> 20
<211> 55
<212> PRT
<213> Fugu rubripes

<400> 20
Ser Gln Leu Gln His Cys Glu Glu Ser Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Leu Gly Glu Gln Glu Leu Lys Asn Gly Val Val Lys Leu Arg Asn Val
            20                  25                  30          
Ala Thr Arg Asp Glu Val Asp Ile Ser Arg Ala Asp Leu Ile Ala Glu
        35                  40                  45              
Ile Lys Lys Arg Thr Ser Ala
    50                  55  


<210> 21
<211> 56
<212> PRT
<213> Caenorhabditis elegans

<400> 21
Thr Gln Phe Gln Tyr Ala Glu Glu Arg Arg Ile Pro Leu Ala Ile Val
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Val Lys Leu Arg Asn Val
            20                  25                  30          
Val Thr Arg Asp Glu Gln Thr Ile Lys Leu Asp Gln Leu Ile Thr Ala
        35                  40                  45              
Val Arg Asp Thr Leu Ala Ala Leu
    50                  55      


<210> 22
<211> 58
<212> PRT
<213> Dictyostelium discoideum

<400> 22
Ala Gln Leu Asn Thr Ala Asp Glu Ser Asn Ile Pro Leu Ile Ile Ile
 1               5                  10                  15      
Ile Gly Lys Ser Glu Val Glu Thr Asn Ser Leu Ser Val Lys Thr Met
            20                  25                  30          
His Asp Arg Lys Gln Val Ser Ile Glu Arg Ser Asn Phe Thr Val Lys
        35                  40                  45              
Ile Lys Glu Ile Leu Ser Thr Ile Pro Lys
    50                  55              


<210> 23
<211> 56
<212> PRT
<213> Oryza sativa subsp. japonica

<400> 23
Asn His Leu Lys Tyr Ala Thr Gln Ser Gly Ile Pro Trp Met Val Leu
 1               5                  10                  15      
Val Gly Glu Ser Glu Ile Ser Ser Gly Lys Val Lys Leu Lys Asn Leu
            20                  25                  30          
Ala Ala Ser Gln Glu Glu Glu Val Asp Arg Thr Glu Phe Ala Gln Val
        35                  40                  45              
Leu Lys Gln Lys Leu Arg Asn Pro
    50                  55      


<210> 24
<211> 53
<212> DNA
<213> Homo sapiens

<400> 24
aagtcattga tacacctgta tttgaactaa aggaaacact gatgggaaag tat        53

<210> 25
<211> 18
<212> PRT
<213> Homo sapiens

<400> 25
Glu Val Ile Asp Thr Pro Val Phe Glu Leu Lys Glu Thr Leu Met Gly
 1               5                  10                  15      
Lys Tyr
        


<210> 26
<211> 62
<212> DNA
<213> Homo sapiens

<400> 26
gtcattgata cacctgtatt tgaactaaag gctttggagg agaagatacg gaccacggag 60
ac                                                                62

<210> 27
<211> 49
<212> DNA
<213> Homo sapiens

<400> 27
ggaacagaga ctagaggctt tggaggagaa gatacggacc acggagaca             49

<210> 28
<211> 16
<212> PRT
<213> Homo sapiens

<400> 28
Glu Gln Arg Leu Glu Ala Leu Glu Glu Lys Ile Arg Thr Thr Glu Thr
 1               5                  10                  15      


<210> 29
<211> 60
<212> PRT
<213> Homo sapiens

<400> 29
Asn Gln Leu Gln Tyr Cys Glu Glu Ala Gly Ile Pro Leu Val Ala Ile
 1               5                  10                  15      
Ile Gly Glu Gln Glu Leu Lys Asp Gly Val Ile Lys Leu Arg Ser Val
            20                  25                  30          
Thr Ser Arg Glu Glu Val Asp Val Arg Arg Glu Asp Leu Val Glu Glu
        35                  40                  45              
Ile Lys Arg Arg Thr Gly Gln Pro Leu Cys Ile Cys
    50                  55                  60  

    

