                         SEQUENCE LISTING

<110>  Board of Regents, The University of Texas System
       Matz, Mikhail V.
       Hunt, Marguerite E.
 
<120>  ISOLATION AND CHARACTERIZATION OF NOVEL GREEN FLUORESCENT 
       PROTEINS FROM COPEPODS

<130>  UTAU:2228

<140>  Concurrently herewith
<141>  2010-03-09

<150>  61/162,112
<151>  2009-03-20

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  222
<212>  PRT
<213>  Pontella mimocerami

<400>  1

Met Pro Asn Met Lys Leu Glu Cys Arg Ile Ser Gly Thr Met Asn Gly 
1               5                   10                  15      


Glu Glu Phe Glu Leu Val Gly Asn Gly Asp Gly Asn Thr Asp Glu Gly 
            20                  25                  30          


Arg Met Thr Asn Lys Met Lys Ser Thr Lys Gly Pro Leu Ser Phe Ser 
        35                  40                  45              


Pro Tyr Leu Leu Ser His Val Leu Gly Tyr Gly Tyr Tyr His Tyr Ala 
    50                  55                  60                  


Thr Phe Pro Ala Gly Tyr Glu Asn Val Tyr Leu His Ala Met Lys Asn 
65                  70                  75                  80  


Gly Gly Tyr Ser Asn Thr Arg Thr Glu Arg Tyr Glu Asp Gly Gly Ile 
                85                  90                  95      


Ile Ser Ala Thr Phe Asn Tyr Arg Tyr Glu Gly Asp Lys Ile Ile Gly 
            100                 105                 110         


Asp Phe Lys Val Val Gly Thr Gly Phe Pro Thr Asn Ser Ile Ile Phe 
        115                 120                 125             


Thr Asp Lys Ile Ile Lys Ser Asn Pro Thr Cys Glu His Ile Tyr Pro 
    130                 135                 140                 


Lys Ala Asp Asn Ile Leu Val Asn Ala Tyr Thr Arg Thr Trp Met Leu 
145                 150                 155                 160 


Arg Asp Gly Gly Tyr Tyr Ser Ala Gln Val Asn Asn His Met His Phe 
                165                 170                 175     


Lys Ser Ala Ile His Pro Thr Met Leu Gln Asn Gly Gly Ser Met Phe 
            180                 185                 190         


Thr Tyr Arg Lys Val Glu Glu Leu His Thr Gln Thr Glu Val Gly Ile 
        195                 200                 205             


Val Glu Tyr Gln His Val Phe Lys Thr Pro Thr Ala Phe Ala 
    210                 215                 220         


<210>  2
<211>  666
<212>  DNA
<213>  Pontella mimocerami

<400>  2
atgcccaaca tgaagcttga gtgccgcatc tccggaacca tgaacggaga ggagtttgaa       60

cttgttggta atggagatgg aaacactgat gagggacgca tgaccaacaa gatgaagtcc      120

accaagggac ctctctcctt ctctccctac ttgctctccc atgttcttgg ctatggatac      180

taccactatg ctaccttccc tgctggatat gaaaatgtct acctccatgc catgaagaat      240

ggaggttact ctaacacaag aactgagagg tatgaggatg gaggtatcat ttctgctacc      300

ttcaactaca gatatgaagg agacaagatc attggggact tcaaggttgt tggaactgga      360

ttccctacca acagcatcat cttcactgac aaaataatca agtccaaccc tacctgtgag      420

cacatctacc ccaaggctga caatattctt gtgaatgcct acaccagaac ctggatgctt      480

agagatggtg gatactactc tgctcaggtc aacaaccaca tgcacttcaa gagtgccatc      540

catcccacca tgctccagaa tggtggatcc atgttcacct acagaaaggt tgaggagctc      600

cacacacaaa ctgaagttgg tattgttgag taccagcatg tcttcaaaac tccaactgct      660

tttgct                                                                 666


<210>  3
<211>  222
<212>  PRT
<213>  Pontella mimocerami

<400>  3

Met Pro Asn Met Lys Leu Glu Cys Arg Ile Ser Gly Thr Met Asn Gly 
1               5                   10                  15      


Glu Glu Phe Lys Leu Val Gly Ala Gly Glu Gly Asn Thr Asp Glu Gly 
            20                  25                  30          


Arg Met Thr Asn Lys Val Lys Ser Thr Lys Gly Pro Leu Pro Phe Ser 
        35                  40                  45              


Pro Tyr Leu Leu Ser His Val Leu Gly Tyr Gly Tyr Tyr His Tyr Ala 
    50                  55                  60                  


Thr Phe Pro Ala Gly Tyr Glu Asn Val Tyr Leu His Ala Met Lys Asn 
65                  70                  75                  80  


Gly Gly Tyr Ser Asn Thr Arg Thr Glu Arg Tyr Glu Asp Gly Gly Ile 
                85                  90                  95      


Ile Ser Ala Thr Phe Asn Tyr Arg Tyr Glu Gly Asp Lys Ile Ile Gly 
            100                 105                 110         


Asp Phe Lys Val Val Gly Thr Gly Phe Pro Thr Asn Ser Ile Ile Phe 
        115                 120                 125             


Thr Asp Lys Ile Ile Lys Ser Asn Pro Thr Cys Glu His Ile Tyr Pro 
    130                 135                 140                 


Lys Ala Asp Asn Ile Leu Val Asn Ala Tyr Thr Arg Thr Trp Met Leu 
145                 150                 155                 160 


Arg Asp Gly Gly Tyr Tyr Ser Ala Gln Val Asn Asn His Met His Phe 
                165                 170                 175     


Lys Ser Ala Ile His Pro Thr Met Leu Gln Asn Gly Gly Ser Met Phe 
            180                 185                 190         


Thr Tyr Arg Lys Val Glu Glu Leu His Thr Gln Thr Glu Val Gly Ile 
        195                 200                 205             


Val Glu Tyr Gln His Val Phe Lys Arg Pro Thr Ala Phe Ala 
    210                 215                 220         


<210>  4
<211>  666
<212>  DNA
<213>  Pontella mimocerami

<400>  4
atgcccaaca tgaagcttga gtgccgtatc tccggaacca tgaacggaga ggagtttaaa       60

cttgttggtg ctggagaagg aaacactgat gagggacgca tgaccaacaa ggtgaagtcc      120

accaagggac ctctcccctt ctctccctac ttgctctctc acgttcttgg ctatggatac      180

taccactatg ctaccttccc tgctggatat gaaaatgtct acctccatgc catgaagaat      240

ggaggttact ccaacaccag aactgagagg tatgaggacg gaggtatcat ttctgctacc      300

ttcaactaca gatatgaagg agacaagatc attggagact tcaaggttgt tggaactgga      360

ttccccacca acagcatcat cttcactgac aagattatca agtccaaccc tacctgtgag      420

cacatatacc ccaaggctga caatattctt gtgaatgcct acaccagaac ctggatgctt      480

agagatggtg gatactactc tgctcaggtc aacaaccaca tgcacttcaa gagtgccatc      540

catcccacca tgctccagaa tggtggatcc atgttcacct acagaaaggt tgaggagctc      600

cacacacaaa ctgaagttgg tattgttgag taccagcatg tcttcaagag gccaactgct      660

tttgct                                                                 666


<210>  5
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant amino acid sequence of pmim GFP1 from Pontella mimocerami

<400>  5

Met Pro Asn Met Glu Leu Glu Cys Arg Ile Ser Gly Thr Met Asn Gly 
1               5                   10                  15      


Glu Glu Phe Glu Leu Val Gly Asn Gly Asp Gly Asn Thr Asp Glu Gly 
            20                  25                  30          


Arg Met Thr Asn Lys Met Lys Ser Thr Lys Gly Pro Leu Ser Phe Ser 
        35                  40                  45              


Pro Tyr Leu Leu Ser His Val Leu Gly Tyr Gly Tyr Tyr His Tyr Ala 
    50                  55                  60                  


Thr Phe Pro Ala Gly Tyr Glu Asn Val Tyr Leu His Ala Met Lys Asn 
65                  70                  75                  80  


Gly Gly Tyr Ser Asn Thr Arg Thr Glu Arg Tyr Glu Asp Gly Gly Ile 
                85                  90                  95      


Ile Ser Ala Thr Phe Asn Tyr Arg Tyr Glu Gly Asp Lys Ile Ile Gly 
            100                 105                 110         


Asp Phe Lys Val Val Gly Thr Gly Phe Pro Thr Asn Ser Ile Ile Phe 
        115                 120                 125             


Thr Asp Lys Ile Ile Lys Ser Asn Pro Thr Cys Glu His Ile Tyr Pro 
    130                 135                 140                 


Lys Ala Asp Asn Ile Leu Val Asn Ala Tyr Thr Arg Thr Trp Met Leu 
145                 150                 155                 160 


Arg Asp Gly Gly Tyr Tyr Ser Ala Gln Val Asn Asn His Met His Phe 
                165                 170                 175     


Lys Ser Ala Ile His Pro Thr Met Leu Gln Asn Gly Gly Ser Met Phe 
            180                 185                 190         


Thr Tyr Arg Lys Val Glu Glu Leu His Thr Gln Thr Glu Val Gly Ile 
        195                 200                 205             


Val Glu Tyr Gln His Val Phe Lys Thr Pro Thr Ala Phe Ala 
    210                 215                 220         


<210>  6
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant nucleic acid sequence of pmim GFP1 from Pontella 
       mimocerami

<400>  6
atgcccaaca tggagcttga gtgccgcatc tccggaacca tgaacggaga ggagtttgaa       60

cttgttggta atggagatgg aaacactgat gagggacgca tgaccaacaa gatgaagtcc      120

accaagggac ctctctcctt ctctccctac ttgctctccc atgttcttgg ctatggatac      180

taccactatg ctaccttccc tgctggatat gaaaatgtct acctccatgc catgaagaat      240

ggaggttact ctaacacaag aactgagagg tatgaggatg gaggtatcat ttctgctacc      300

ttcaactaca gatatgaagg agacaagatc attggggact tcaaggttgt tggaactgga      360

ttccctacca acagcatcat cttcactgac aaaataatca agtccaaccc tacctgtgag      420

cacatctacc ccaaggctga caatattctt gtgaatgcct acaccagaac ctggatgctt      480

agagatggtg gatactactc tgctcaggtc aacaaccaca tgcacttcaa gagtgccatc      540

catcccacca tgctccagaa tggtggatcc atgttcacct acagaaaggt tgaggagctc      600

cacacacaaa ctgaagttgg tattgttgag taccagcatg tcttcaaaac tccaactgct      660

tttgct                                                                 666


<210>  7
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant amino acid sequence of pmim GFP1 from Pontella mimocerami

<400>  7

Met Pro Asn Met Thr Leu Glu Ser Ala Ile Ser Gly Thr Met Asn Gly 
1               5                   10                  15      


Glu Glu Phe Glu Leu Val Gly Asn Gly Asp Gly Asn Thr Asp Glu Gly 
            20                  25                  30          


Arg Met Thr Asn Lys Met Lys Ser Thr Lys Gly Pro Leu Ser Phe Ser 
        35                  40                  45              


Pro Tyr Leu Leu Ser His Val Leu Gly Tyr Gly Tyr Tyr His Tyr Ala 
    50                  55                  60                  


Thr Phe Pro Ala Gly Tyr Glu Asn Val Tyr Leu His Ala Met Lys Asn 
65                  70                  75                  80  


Gly Gly Tyr Ser Asn Thr Arg Thr Glu Arg Tyr Glu Asp Gly Gly Ile 
                85                  90                  95      


Ile Ser Ala Thr Phe Asn Tyr Arg Tyr Glu Gly Asp Lys Ile Ile Gly 
            100                 105                 110         


Asp Phe Lys Val Val Gly Thr Gly Phe Pro Thr Asn Ser Ile Ile Phe 
        115                 120                 125             


Thr Asp Lys Ile Ile Lys Ser Asn Pro Thr Cys Glu His Ile Tyr Pro 
    130                 135                 140                 


Lys Ala Asp Asn Ile Leu Val Asn Ala Tyr Thr Arg Thr Trp Met Leu 
145                 150                 155                 160 


Arg Asp Gly Gly Tyr Tyr Ser Ala Gln Val Asn Asn His Met His Phe 
                165                 170                 175     


Lys Ser Ala Ile His Pro Thr Met Leu Gln Asn Gly Gly Ser Met Phe 
            180                 185                 190         


Thr Tyr Arg Lys Val Glu Glu Leu His Thr Gln Thr Glu Val Gly Ile 
        195                 200                 205             


Val Glu Tyr Gln His Val Phe Lys Thr Pro Thr Ala Phe Ala 
    210                 215                 220         


<210>  8
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant nucleic acid sequence of pmim GFP1 from Pontella 
       mimocerami

<400>  8
atgcccaaca tgacgcttga gtccgccatc tccggaacca tgaacggaga ggagtttgaa       60

cttgttggta atggagatgg aaacactgat gagggacgca tgaccaacaa gatgaagtcc      120

accaagggac ctctctcctt ctctccctac ttgctctccc atgttcttgg ctatggatac      180

taccactatg ctaccttccc tgctggatat gaaaatgtct acctccatgc catgaagaat      240

ggaggttact ctaacacaag aactgagagg tatgaggatg gaggtatcat ttctgctacc      300

ttcaactaca gatatgaagg agacaagatc attggggact tcaaggttgt tggaactgga      360

ttccctacca acagcatcat cttcactgac aaaataatca agtccaaccc tacctgtgag      420

cacatctacc ccaaggctga caatattctt gtgaatgcct acaccagaac ctggatgctt      480

agagatggtg gatactactc tgctcaggtc aacaaccaca tgcacttcaa gagtgccatc      540

catcccacca tgctccagaa tggtggatcc atgttcacct acagaaaggt tgaggagctc      600

cacacacaaa ctgaagttgg tattgttgag taccagcatg tcttcaaaac tccaactgct      660

tttgct                                                                 666


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide for PCR

<400>  9
aagcagtggt atcaacgcag agtcgcagtc ggtactv                                37


<210>  10
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide for PCR

<400>  10
agtggactat ccatgaacgc aaagcagtgg tatcaacgca gagt                        44


<210>  11
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide for PCR

<400>  11
ttgattgatt gaaggagaaa tatcatg                                           27


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide for PCR

<400>  12
catcaccatc accatcacta aa                                                22


