                         SEQUENCE LISTING

<110>  Gonzalez Grassi, Federico
       Izpisua Belmonte, Juan Carlos
       The Salk Institute for Biological Studies
 
<120>  Generation of Mouse induced-Pluripotent Stem Cells by Transient 
       Expression of a Single Non-Viral Polycistronic Vector

<130>  027731-000710PC

<150>  US 61/160,263
<151>  2009-03-13

<160>  85    

<170>  PatentIn version 3.3

<210>  1
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for probe generation 
       for Probe A for Oct4

<400>  1
tgggggtgat gggtcagca                                                  19


<210>  2
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for probe generation 
       for Probe A for Oct4

<400>  2
ctcctcggga gttggttcc                                                  19


<210>  3
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for probe generation 
       for Probe B for Sox2

<400>  3
acagctacgc gcacatgaac                                                 20


<210>  4
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for probe generation 
       for Probe B for Sox2

<400>  4
atgtgcgaca ggggcagtg                                                  19


<210>  5
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for probe generation 
       for Probe C for Klf4

<400>  5
atcaatgacg tgagcccctc                                                 20


<210>  6
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for probe generation 
       for Probe C for Klf4

<400>  6
tagtcctggt ggggagctg                                                  19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for probe generation 
       for Probe D for Myc

<400>  7
gtcgctacgt ccttctccc                                                  19


<210>  8
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for probe generation 
       for Probe D for Myc

<400>  8
cgaggtacag gatttgggcg                                                 20


<210>  9
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 1

<400>  9
ttgctggcgt ttttccatag g                                               21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 1

<400>  10
ctacatacct cgctctgcta a                                               21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 2

<400>  11
agcagcagat tacgcgcaga                                                 20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 2

<400>  12
tactctagct tcccggcaac                                                 20


<210>  13
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 3

<400>  13
acctatctca gcgatctgtc t                                               21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 3

<400>  14
cggccaactt acttctgaca a                                               21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 4

<400>  15
ttgtcagaag taagttggcc g                                               21


<210>  16
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 4

<400>  16
gccttcttga cgagttcttc t                                               21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 5

<400>  17
agaagaactc gtcaagaagg c                                               21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 5

<400>  18
ttgctcctgc cgagaaagta t                                               21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 6

<400>  19
atactttctc ggcaggagca a                                               21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 6

<400>  20
cttacatggc gatagctaga c                                               21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 7

<400>  21
attgtctgtt gtgcccagtc a                                               21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 7

<400>  22
actggaacaa cactcaaccc t                                               21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 8

<400>  23
gtctagctat cgccatgtaa g                                               21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 8

<400>  24
ctttccccgt caagctctaa a                                               21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 9

<400>  25
tttagagctt gacggggaaa g                                               21


<210>  26
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 9

<400>  26
ctggcgttac ccaacttaat c                                               21


<210>  27
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 10

<400>  27
gctgcgcgta accaccaca                                                  19


<210>  28
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 10

<400>  28
gaactaatga ccccgtaatt ga                                              22


<210>  29
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 11

<400>  29
gattaagttg ggtaacgcca g                                               21


<210>  30
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 11

<400>  30
ggcgtacttg gcatatgata c                                               21


<210>  31
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 12

<400>  31
tcaattacgg ggtcattagt tc                                              22


<210>  32
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 12

<400>  32
atacaaaatt gggggtgggg a                                               21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 13

<400>  33
ttatggtaat cgtgcgagag g                                               21


<210>  34
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 13

<400>  34
caataaccag cacgttgccc                                                 20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 14

<400>  35
gggcaacgtg ctggttattg                                                 20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 14

<400>  36
tgccatccct ccgcagaact                                                 20


<210>  37
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 15

<400>  37
ctgctggaga agtgggtgg                                                  19


<210>  38
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 15

<400>  38
agaaacatgg tctccagact c                                               21


<210>  39
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 16

<400>  39
ttccctctgt tcccgtcact                                                 20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 16

<400>  40
gcttgctgat ctccgagttg                                                 20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 17

<400>  41
ttacctcttc ctcccactcc                                                 20


<210>  42
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 17

<400>  42
gcttcatgtg agagagttcc t                                               21


<210>  43
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 18

<400>  43
tctgctcccg tccttctcc                                                  19


<210>  44
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 18

<400>  44
gtcgttgaac tcctcggtct                                                 20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 19

<400>  45
aacacacacg acttccccct                                                 20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 19

<400>  46
gtgggtggct gttcttttcc                                                 20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 20

<400>  47
tgaactgacc aggcactacc                                                 20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 20

<400>  48
ctcttcctcg tcgcagatga                                                 20


<210>  49
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 21

<400>  49
ctagagcttg cggaaccctt a                                               21


<210>  50
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 21

<400>  50
ccaatacgca aaccgcctct                                                 20


<210>  51
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number 22

<400>  51
gttatccgct cacaattcca c                                               21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number 22

<400>  52
cctatggaaa aacgccagca a                                               21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer to detect integration 
       of the transgene for primer pair number E

<400>  53
ctgatttggt tggtgtcttg c                                               21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer to detect integration 
       of the transgene for primer pair number E

<400>  54
aattccttac cagcctctgt g                                               21


<210>  55
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Oct4 5' UTR

<400>  55
taggtgagcc gtctttccac                                                 20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Oct4 5' UTR

<400>  56
gcttagccag gttcgaggat                                                 20


<210>  57
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Sox2 3' UTR

<400>  57
gaaacgacag ctgcggaaa                                                  19


<210>  58
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Sox2 3' UTR

<400>  58
tctagtcggc atcacggttt t                                               21


<210>  59
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Nanog ex1-2 junction

<400>  59
ttgcttacaa gggtctgcta ct                                              22


<210>  60
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Nanog ex1-2 junction

<400>  60
actggtagaa gaatcagggc t                                               21


<210>  61
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Utf1 ex2-3 3' UTR junction

<400>  61
acgtggagca tctacgaggt                                                 20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Utf1 ex2-3 3' UTR junction

<400>  62
tagactggga gtcgtttctg                                                 20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Zfp42 3' UTR

<400>  63
tgtcctcagg ctgggtagtc                                                 20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Zfp42 3' UTR

<400>  64
tgattttccg acgtatgcaa                                                 20


<210>  65
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Col6a2 ex2-3 junction

<400>  65
ccaccactga aaggaacaac aa                                              22


<210>  66
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Col6a2 ex2-3 junction

<400>  66
tccaacacga aatacacgtt gac                                             23


<210>  67
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Gremlin2 ex2-3' UTR junction

<400>  67
gaagcattgc cggtgcat                                                   18


<210>  68
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Gremlin2 ex2-3' UTR junction

<400>  68
tcctgcccca tgagttcac                                                  19


<210>  69
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Thy1 ex1-2 junction

<400>  69
ggaactcttg gcaccatgaa c                                               21


<210>  70
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Thy1 ex1-2 junction

<400>  70
gggacacctg caagactgag a                                               21


<210>  71
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Gapdh ex2-3 junction

<400>  71
aggtcggtgt gaacggattt g                                               21


<210>  72
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Gapdh ex2-3 junction

<400>  72
tgtagaccat gtagttgagg tca                                             23


<210>  73
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for forward primer for quantitative RT-PCR
       for Transgene Sox2-Klf4 junction

<400>  73
actgcccctg tcgcacat                                                   18


<210>  74
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for reverse primer for quantitative RT-PCR
       for Transgene Sox2-Klf4 junction

<400>  74
gcgtcgctga cagccatag                                                  19


<210>  75
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name mOctF

<400>  75
cgccaccatg gctggacacc tggctt                                          26


<210>  76
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name Bsp mOct R

<400>  76
tccggatccg tttgaatgca tgggagagc                                       29


<210>  77
<211>  85
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name AgPTV mSox F

<400>  77
accggtgcca cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt     60

cctatgtata acatgatgga gacgg                                           85


<210>  78
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name Bsp mSox R

<400>  78
tccggatccc atgtgcgaca ggggcagt                                        28


<210>  79
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name AgPTV mKlf F

<400>  79
accggtgcca cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt     60

cctatggctg tcagcgacgc tc                                              82


<210>  80
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name Bsp mKlf R

<400>  80
tccggatcca aagtgcctct tcatgtgtaa g                                    31


<210>  81
<211>  83
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name AgPTV mMyc F

<400>  81
accggtgcca cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt     60

cctatgcccc tcaacgtgaa ctt                                             83


<210>  82
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name Bsp mMyc R

<400>  82
tccggatcct gcaccagagt ttcgaagct                                       29


<210>  83
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name Myc R

<400>  83
ttatgcacca gagtttcgaa gct                                             23


<210>  84
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name AgPTV GFP F

<400>  84
accggtgcca cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt     60

cctatggtga gcaagggcga gg                                              82


<210>  85
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence for primer for construct generation for 
       primer name GFP R

<400>  85
ttacttgtac agctcgtcca t                                               21


