                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Method for Improving the Yield of a Polypeptide

<130>  27179-WO-PCT

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  1137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized sequence

<400>  1
ttcttaggta agatcgctaa gaacccagaa gcctctatga acgtttccca aatgatttct       60

tactggggtt acccatctga aatgcacaag gtcattaccg ctgacggtta cattttgcaa      120

gtttaccgta tcccacacgg taagaacaac gctaaccatt tgggtcaaag accagttgtc      180

tttttgcaac acggtttatt gggttccgct actaactgga tctccaattt gccaaagaac      240

tctctaggtt tcttgttggc tgatgctggt tacgatgtct ggttaggtaa ctctagaggt      300

aacacctggg ctcaagaaca cttgtactac tctccagact ccccagaatt ctgggctttc      360

tccttcgacg aaatggctga atacgactta ccatctacta tcgacttcat tttgagaaga      420

actggtcaaa agaaattgca ttacgttggt cactcccaag gtactactat cggtttcatt      480

gccttctcca cctccccaac cttggctgaa aagatcaagg tcttctacgc tttagcccca      540

gttgctactg ttaagtacac caaatctttg ttcaacaaat tggctttgat cccacacttc      600

ttgttcaaga ttatctttgg tgacaagatg ttctatccac acaccttttt ggaacaattc      660

ttgggtgtcg aaatgtgttc tagagaaacc ttggatgtct tgtgtaagaa cgctttattc      720

gctatcactg gtgttgacaa caagaacttt aacatgtcta gattggatgt ttacattgcc      780

cacaacccag ccggtacctc cgtccaaaac accttgcact ggagacaagc tgttaagtct      840

ggtaagttcc aagccttcga ctggggtgcc ccataccaaa acttgatgca ctaccaccaa      900

ccaactcctc caatttacaa tttgaccgct atgaacgttc caattgccgt ctggtccgct      960

gataacgatt tgctagctga ccctcaagat gtcgacttct tgttgtctaa gttgtctaac     1020

ttgatctacc acaaggaaat cccaaactac aaccatttgg atttcatctg ggctatggac     1080

gctccacaag aagtttacaa cgaaatcgtc tctttgatgg ctgaagacaa gaaataa        1137


<210>  2
<211>  397
<212>  PRT
<213>  Calf

<400>  2

Met Trp Trp Leu Leu Val Thr Val Cys Phe Ile His Met Ser Gly Asn 
1               5                   10                  15      


Ala Phe Cys Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met 
            20                  25                  30          


Asn Val Ser Gln Met Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Met His 
        35                  40                  45              


Lys Val Ile Thr Ala Asp Gly Tyr Ile Leu Gln Val Tyr Arg Ile Pro 
    50                  55                  60                  


His Gly Lys Asn Asn Ala Asn His Leu Gly Gln Arg Pro Val Val Phe 
65                  70                  75                  80  


Leu Gln His Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu 
                85                  90                  95      


Pro Lys Asn Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val 
            100                 105                 110         


Trp Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Gln Glu His Leu Tyr 
        115                 120                 125             


Tyr Ser Pro Asp Ser Pro Glu Phe Trp Ala Phe Ser Phe Asp Glu Met 
    130                 135                 140                 


Ala Glu Tyr Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Arg Arg Thr 
145                 150                 155                 160 


Gly Gln Lys Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile 
                165                 170                 175     


Gly Phe Ile Ala Phe Ser Thr Ser Pro Thr Leu Ala Glu Lys Ile Lys 
            180                 185                 190         


Val Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser 
        195                 200                 205             


Leu Phe Asn Lys Leu Ala Leu Ile Pro His Phe Leu Phe Lys Ile Ile 
    210                 215                 220                 


Phe Gly Asp Lys Met Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu 
225                 230                 235                 240 


Gly Val Glu Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn 
                245                 250                 255     


Ala Leu Phe Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser 
            260                 265                 270         


Arg Leu Asp Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln 
        275                 280                 285             


Asn Thr Leu His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala 
    290                 295                 300                 


Phe Asp Trp Gly Ala Pro Tyr Gln Asn Leu Met His Tyr His Gln Pro 
305                 310                 315                 320 


Thr Pro Pro Ile Tyr Asn Leu Thr Ala Met Asn Val Pro Ile Ala Val 
                325                 330                 335     


Trp Ser Ala Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Phe 
            340                 345                 350         


Leu Leu Ser Lys Leu Ser Asn Leu Ile Tyr His Lys Glu Ile Pro Asn 
        355                 360                 365             


Tyr Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Glu Val 
    370                 375                 380                 


Tyr Asn Glu Ile Val Ser Leu Met Ala Glu Asp Lys Lys 
385                 390                 395         


<210>  3
<211>  1137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE protein feature optimized (PFO) variant KL8, 1 extra 
       glycosylation site added

<400>  3
tttttgggta agattgccaa gaacccagaa gcctccatga acgtttctca aatgatctct       60

tactggggtt acccatctga aatgcacaag gttatcactg ctgacggtta catcttacaa      120

gtctacagaa tcccacacgg taagaacaat gctaaccatt tgggtcaaag accagttgtt      180

ttcttgcaac atggtctatt aggttctgcc accaactgga tctccaactt gccaaacaac      240

tctttgggtt tcttgttggc tgatgctggt tacgatgtct ggttaggtaa ctccagaggt      300

aacacctggg ctcaagaaca cttgtactac tctccagatt ctccagaatt ctgggctttc      360

tctttcgacg aaatggctga atacgacttg ccatctacca ttgacttcat cttgagaaga      420

accggtcaaa agaaattgca ctacgttggt cactctcaag gtaccaccat tggtttcatt      480

gctttctcca cttctccaac tttggctgaa aagatcaagg ttttctacgc tttggctcca      540

gttgccaccg tcaagtacac caaatcttta ttcaacaaat tggctttgat tccacacttc      600

ttattcaaga tcatcttcgg tgacaagatg ttctatcctc acactttctt ggaacaattc      660

ttgggtgttg aaatgtgttc cagagaaact ttggatgtct tgtgtaagaa cgctttgttt      720

gccatcactg gtgttgacaa caagaacttc aacatgtccc gtttggatgt ctacattgct      780

cacaacccag ctggtacttc cgttcaaaac actttgcact ggagacaagc tgtcaaatct      840

ggtaagttcc aagcctttga ctggggtgct ccataccaaa acttgatgca ctaccaccaa      900

ccaaccccac caatctacaa cttgactgcc atgaacgttc caattgctgt ctggtccgct      960

gacaacgatt tgttggctga ccctcaagat gtcgacttct tgctatccaa gttgtccaac     1020

ttgatctacc acaaggaaat tccaaactac aaccatttgg atttcatctg ggctatggac     1080

gctccacaag aagtctacaa cgaaattgtc tctttgatgg ctgaagacaa gaagtaa        1137


<210>  4
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL8, 1 extra glycosylation site added

<400>  4

Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met Asn Val Ser 
1               5                   10                  15      


Gln Met Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Met His Lys Val Ile 
            20                  25                  30          


Thr Ala Asp Gly Tyr Ile Leu Gln Val Tyr Arg Ile Pro His Gly Lys 
        35                  40                  45              


Asn Asn Ala Asn His Leu Gly Gln Arg Pro Val Val Phe Leu Gln His 
    50                  55                  60                  


Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro Asn Asn 
65                  70                  75                  80  


Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly 
                85                  90                  95      


Asn Ser Arg Gly Asn Thr Trp Ala Gln Glu His Leu Tyr Tyr Ser Pro 
            100                 105                 110         


Asp Ser Pro Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Glu Tyr 
        115                 120                 125             


Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Arg Arg Thr Gly Gln Lys 
    130                 135                 140                 


Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Phe Ser Thr Ser Pro Thr Leu Ala Glu Lys Ile Lys Val Phe Tyr 
                165                 170                 175     


Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn 
            180                 185                 190         


Lys Leu Ala Leu Ile Pro His Phe Leu Phe Lys Ile Ile Phe Gly Asp 
        195                 200                 205             


Lys Met Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu Gly Val Glu 
    210                 215                 220                 


Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn Ala Leu Phe 
225                 230                 235                 240 


Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp 
                245                 250                 255     


Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu 
            260                 265                 270         


His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe Asp Trp 
        275                 280                 285             


Gly Ala Pro Tyr Gln Asn Leu Met His Tyr His Gln Pro Thr Pro Pro 
    290                 295                 300                 


Ile Tyr Asn Leu Thr Ala Met Asn Val Pro Ile Ala Val Trp Ser Ala 
305                 310                 315                 320 


Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Phe Leu Leu Ser 
                325                 330                 335     


Lys Leu Ser Asn Leu Ile Tyr His Lys Glu Ile Pro Asn Tyr Asn His 
            340                 345                 350         


Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Glu Val Tyr Asn Glu 
        355                 360                 365             


Ile Val Ser Leu Met Ala Glu Asp Lys Lys 
    370                 375             


<210>  5
<211>  1137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL9, 5 extra glycosylation sites added

<400>  5
tttttgggta agattgccaa gaacccagaa gcctccatga acgtttctca aatgatctct       60

tactggggtt acccatctga aatgcacaag gtcatcactg ctgatggtta catcttacaa      120

gtctacagaa ttccacacgg taagaacaac tccaaccatt tgggtcaaag accagttgtc      180

tttttgcaac acggtctatt aggttctgct accaactgga tctccaactt gccaaacaac      240

tctttgggtt tcttgttggc tgatgccggt tacgatgtct ggttaggtaa ctcccgtggt      300

aacacctggg ctcaagaaca cttgtactac tctccagact ctccagaatt ctgggctttc      360

tctttcgacg aaatggctga atacgacttg ccatctacca ttgacttcat cttgaacaag      420

accggtcaaa agaagttgca ctacgttggt cactctcaag gtaccaccat tggtttcatc      480

gctttctcca cttctccaac tttggctgaa aagatcaagg ttttctacgc tttggctcca      540

gttgctaccg tcaagtacac caaatcttta ttcaacaaat tggctttgat tccacacttc      600

ttattcaaga tcatcttcgg tgacaagatg ttctacccac acactttctt ggaacaattc      660

ttgggtgttg aaatgtgttc cagagaaact ttggatgtct tgtgtaagaa cgctttgttt      720

gccatcactg gtgttgacaa caagaacttc aacatgtcca gattagatgt ctacattgct      780

cacaacccag ccggtacttc tgttcaaaac actttgcact ggagacaagc tgtcaagtct      840

ggtaagttcc aagccttcga ctggggtgct ccataccaaa acttgatgca ctacaaccaa      900

tccactccac ctatctacaa cttgactgcc atgaacgttc caattgctgt ctggtctgct      960

gacaacgatt tgttggctga ccctcaagat gtcgatttct tgctatccaa gttgtccaac     1020

ttgacctacc acaaggaaat tccaaactac aaccatttgg acttcatctg ggctatggac     1080

gctccacaag aagtttacaa cgaaatcgtt tctttgatgg ctgaagacaa aaaataa        1137


<210>  6
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL9, 5 extra glycosylation sites added

<400>  6

Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met Asn Val Ser 
1               5                   10                  15      


Gln Met Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Met His Lys Val Ile 
            20                  25                  30          


Thr Ala Asp Gly Tyr Ile Leu Gln Val Tyr Arg Ile Pro His Gly Lys 
        35                  40                  45              


Asn Asn Ser Asn His Leu Gly Gln Arg Pro Val Val Phe Leu Gln His 
    50                  55                  60                  


Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro Asn Asn 
65                  70                  75                  80  


Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly 
                85                  90                  95      


Asn Ser Arg Gly Asn Thr Trp Ala Gln Glu His Leu Tyr Tyr Ser Pro 
            100                 105                 110         


Asp Ser Pro Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Glu Tyr 
        115                 120                 125             


Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Asn Lys Thr Gly Gln Lys 
    130                 135                 140                 


Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Phe Ser Thr Ser Pro Thr Leu Ala Glu Lys Ile Lys Val Phe Tyr 
                165                 170                 175     


Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn 
            180                 185                 190         


Lys Leu Ala Leu Ile Pro His Phe Leu Phe Lys Ile Ile Phe Gly Asp 
        195                 200                 205             


Lys Met Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu Gly Val Glu 
    210                 215                 220                 


Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn Ala Leu Phe 
225                 230                 235                 240 


Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp 
                245                 250                 255     


Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu 
            260                 265                 270         


His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe Asp Trp 
        275                 280                 285             


Gly Ala Pro Tyr Gln Asn Leu Met His Tyr Asn Gln Ser Thr Pro Pro 
    290                 295                 300                 


Ile Tyr Asn Leu Thr Ala Met Asn Val Pro Ile Ala Val Trp Ser Ala 
305                 310                 315                 320 


Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Phe Leu Leu Ser 
                325                 330                 335     


Lys Leu Ser Asn Leu Thr Tyr His Lys Glu Ile Pro Asn Tyr Asn His 
            340                 345                 350         


Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Glu Val Tyr Asn Glu 
        355                 360                 365             


Ile Val Ser Leu Met Ala Glu Asp Lys Lys 
    370                 375             


<210>  7
<211>  1137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL11, pI shift of 6.96 to 7.74

<400>  7
tttttgggta agattgccaa gaacccagaa gcctccatga acgtctctca aatgatctct       60

tactggggtt acccatctga agaacacaag gttaccactg aagacggtta catcttggaa      120

gtcaacagaa ttccacacgg taagaagaac tctgaaaaca ccggtcaaag acctgttgtt      180

ttcttgcaac acggtctatt gggttctgct accaactgga tctccaactt gccaaagaac      240

tctttaggtt tcttgttggc tgacgctggt tacgatgtct ggttaggtaa ctcccgtggt      300

aacacctggg ctagaaagca tttgtactac tctccagatt ccaaggaatt ctgggctttc      360

tctttcgatg aaatggccaa gtacgacttg ccatctacca ttgacttcat cttgagaaga      420

accggtcaaa agaaattgca ctacgttggt cactctcaag gtaccaccat cggtttcatt      480

gctttctcca cttctccaga attggctgaa aagatcaaga ctttctacgc tttggctcca      540

gttgctaccg ttaagtacac caaatcttta ttcaacaaat tggctttgat tccacacttc      600

ttgttcaaga tcattttcgg tgacaagatg ttctacccac acactttctt ggaacaattt      660

ttgggtgtcg aaatgtgttc cagagaaact ttggatgtct tatgtaagaa cgctttgttt      720

gccatcactg gtgttgacaa caagaacttc aacatgtcca gattagatgt ttacattgct      780

cacaacccag ccggtacttc cgttcaaaac actttgcact ggagacaagc tgtcaaatct      840

ggtaagttcc aagcctacga ctggggttct ccagaccaaa acagaatgca ctaccatcaa      900

tccactccac caatctacaa cttgactgct atgaacgttc caactgctgt ctggtctgct      960

gacaacgatt tgttggctga ccctcaagat gtcaagaact tgctatccaa gttgtccaac     1020

ttgatctacc acaaggaaat tccaaactac aaccatttgg acttcatctg gggtgaagat     1080

gctccacaag aagtttacaa cgaaatcgtc tctttgatga aggaagacaa gaaataa        1137


<210>  8
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL11, pI shift of 6.96 to 7.74

<400>  8

Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met Asn Val Ser 
1               5                   10                  15      


Gln Met Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Glu His Lys Val Thr 
            20                  25                  30          


Thr Glu Asp Gly Tyr Ile Leu Glu Val Asn Arg Ile Pro His Gly Lys 
        35                  40                  45              


Lys Asn Ser Glu Asn Thr Gly Gln Arg Pro Val Val Phe Leu Gln His 
    50                  55                  60                  


Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro Lys Asn 
65                  70                  75                  80  


Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly 
                85                  90                  95      


Asn Ser Arg Gly Asn Thr Trp Ala Arg Lys His Leu Tyr Tyr Ser Pro 
            100                 105                 110         


Asp Ser Lys Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Lys Tyr 
        115                 120                 125             


Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Arg Arg Thr Gly Gln Lys 
    130                 135                 140                 


Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Phe Ser Thr Ser Pro Glu Leu Ala Glu Lys Ile Lys Thr Phe Tyr 
                165                 170                 175     


Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn 
            180                 185                 190         


Lys Leu Ala Leu Ile Pro His Phe Leu Phe Lys Ile Ile Phe Gly Asp 
        195                 200                 205             


Lys Met Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu Gly Val Glu 
    210                 215                 220                 


Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn Ala Leu Phe 
225                 230                 235                 240 


Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp 
                245                 250                 255     


Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu 
            260                 265                 270         


His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Tyr Asp Trp 
        275                 280                 285             


Gly Ser Pro Asp Gln Asn Arg Met His Tyr His Gln Ser Thr Pro Pro 
    290                 295                 300                 


Ile Tyr Asn Leu Thr Ala Met Asn Val Pro Thr Ala Val Trp Ser Ala 
305                 310                 315                 320 


Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Lys Asn Leu Leu Ser 
                325                 330                 335     


Lys Leu Ser Asn Leu Ile Tyr His Lys Glu Ile Pro Asn Tyr Asn His 
            340                 345                 350         


Leu Asp Phe Ile Trp Gly Glu Asp Ala Pro Gln Glu Val Tyr Asn Glu 
        355                 360                 365             


Ile Val Ser Leu Met Lys Glu Asp Lys Lys 
    370                 375             


<210>  9
<211>  1137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL12, pI shift from 6.96 to 6.7

<400>  9
tttttgggta agattgccaa gaacccagaa gcctccatga acgtttctga aatcatctct       60

tactggggtt acccatctga agaacacgaa gttaccactg aagatggtta cattttggaa      120

gttaaccgta ttccacacgg taagaagaac tctgaacaca ccggtaagag acctgttgtt      180

ttcttacaac acggtctatt aggttctgct accaactgga tctctaactt gccaaagaac      240

tctttgggtt tcttgttggc tgatgctggt tacgatgtct ggttaggtaa ctccagaggt      300

aacacctggt ccagaaagca caagacttta tctccagact ccaaggaatt ctgggctttc      360

tctttcgacg aaatggccaa gtacgatttg ccatctacca ttgacttcat cttaaagaag      420

actggtcaaa agaaattgca ctacgtcggt cactctcaag gtaccaccat tggtttcatc      480

gctttctcca cctctccaga attggccaag aagatcaaga ctttctacgc tttggctcca      540

gttgctaccg tcaaatacac caaatcttta ttcaacaaat tggctcattt gccagaattt      600

ttgttcaagg acttgttcgg tgacaaggaa ttctacccac acactttctt ggaacaattc      660

ttgggtgttg aaatgtgttc cagagaaact ttggatgtct tgtgtaagaa cgctttgttt      720

gccatcactg gtgtcgacaa caagaacttc aacatgtcca gattggatgt ttacattgct      780

cacaacccag ctggtacttc cgtccaaaac actttgcact ggagacaagc tgtcaaatct      840

ggtaagttcc aagccttcga ctggggttct ccagaccaaa acatgaagca ctaccatcaa      900

tccactccac cagaatacaa cgttaccgac atgaaggttc caactgctgt ctggtctgct      960

gacaacgatt tgttggctga ccctcaagat gttgacttct tgttgtccaa attgtccaac     1020

ttgatctacc acaaggaaat cccacactac aaccatttgg acttcatctg gggtgaagat     1080

gctccacaag aagtctacaa cgaaattatc agattgatga aggaagacaa gaagtaa        1137


<210>  10
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE PFO variant KL12, pI shift from 6.96 to 6.7

<400>  10

Phe Leu Gly Lys Ile Ala Lys Asn Pro Glu Ala Ser Met Asn Val Ser 
1               5                   10                  15      


Glu Ile Ile Ser Tyr Trp Gly Tyr Pro Ser Glu Glu His Glu Val Thr 
            20                  25                  30          


Thr Glu Asp Gly Tyr Ile Leu Glu Val Asn Arg Ile Pro His Gly Lys 
        35                  40                  45              


Lys Asn Ser Glu His Thr Gly Lys Arg Pro Val Val Phe Leu Gln His 
    50                  55                  60                  


Gly Leu Leu Gly Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro Lys Asn 
65                  70                  75                  80  


Ser Leu Gly Phe Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly 
                85                  90                  95      


Asn Ser Arg Gly Asn Thr Trp Ser Arg Lys His Lys Thr Leu Ser Pro 
            100                 105                 110         


Asp Ser Lys Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Lys Tyr 
        115                 120                 125             


Asp Leu Pro Ser Thr Ile Asp Phe Ile Leu Lys Lys Thr Gly Gln Lys 
    130                 135                 140                 


Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Phe Ser Thr Ser Pro Glu Leu Ala Lys Lys Ile Lys Thr Phe Tyr 
                165                 170                 175     


Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn 
            180                 185                 190         


Lys Leu Ala His Leu Pro Glu Phe Leu Phe Lys Asp Leu Phe Gly Asp 
        195                 200                 205             


Lys Glu Phe Tyr Pro His Thr Phe Leu Glu Gln Phe Leu Gly Val Glu 
    210                 215                 220                 


Met Cys Ser Arg Glu Thr Leu Asp Val Leu Cys Lys Asn Ala Leu Phe 
225                 230                 235                 240 


Ala Ile Thr Gly Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp 
                245                 250                 255     


Val Tyr Ile Ala His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu 
            260                 265                 270         


His Trp Arg Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe Asp Trp 
        275                 280                 285             


Gly Ser Pro Asp Gln Asn Met Lys His Tyr His Gln Ser Thr Pro Pro 
    290                 295                 300                 


Glu Tyr Asn Val Thr Asp Met Lys Val Pro Thr Ala Val Trp Ser Ala 
305                 310                 315                 320 


Asp Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Phe Leu Leu Ser 
                325                 330                 335     


Lys Leu Ser Asn Leu Ile Tyr His Lys Glu Ile Pro His Tyr Asn His 
            340                 345                 350         


Leu Asp Phe Ile Trp Gly Glu Asp Ala Pro Gln Glu Val Tyr Asn Glu 
        355                 360                 365             


Ile Ile Arg Leu Met Lys Glu Asp Lys Lys 
    370                 375             


<210>  11
<211>  1191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE variant with native signal sequence fused to  -MAT factor 
       signal pre(pro-)sequence

<400>  11
atgtggtggt tattggttac cgtttgtttc attcacatgt ccggtaacgc tttctgtttc       60

ttgggtaaga tcgccaagaa cccagaagcc tccatgaacg tttcccaaat gatctcttac      120

tggggttacc catctgaaat gcacaaggtc atcactgctg acggttacat cttacaagtc      180

tacagaatcc cacacggtaa gaacaatgcc aaccatttgg gtcaaagacc agttgttttc      240

ttgcaacacg gtctattagg ttctgctacc aactggatct ccaacttgcc aaagaactct      300

ttgggtttct tgttggctga tgctggttac gatgtctggt tgggtaactc cagaggtaac      360

acctgggctc aagaacactt gtactactct ccagattctc cagaattctg ggctttctcc      420

ttcgacgaaa tggctgaata cgacttgcca tctaccattg acttcatctt gagaagaacc      480

ggtcaaaaga aattgcacta cgttggtcac tctcaaggta ccaccattgg tttcattgct      540

ttctccactt ctccaacttt ggctgaaaag atcaaggttt tctacgcttt ggctccagtt      600

gccaccgtca agtacaccaa gtctttattc aacaaattgg ctttgattcc acacttcttg      660

ttcaagatca tctttggtga caagatgttc tacccacaca ctttcttgga acaattcttg      720

ggtgttgaaa tgtgttctcg tgaaactttg gatgttctat gtaagaacgc tttgtttgcc      780

atcactggtg ttgacaacaa gaacttcaac atgtccagat tggacgtcta cattgctcac      840

aacccagccg gtacttctgt tcaaaacact ttgcactgga gacaagctgt caaatctggt      900

aagttccaag cctttgactg gggtgctcca taccaaaact tgatgcacta ccatcaacca      960

actccaccaa tttacaactt gactgccatg aacgttccaa ttgctgtctg gtctgctgac     1020

aacgacttat tagctgatcc tcaagatgtc gatttcttgt tgtccaagtt gtccaacttg     1080

atctaccaca aggaaatccc aaactacaac catttggatt tcatctgggc tatggacgct     1140

cctcaagaag tctacaacga aattgtctct ttgatggctg aagacaagaa a              1191


<210>  12
<211>  2710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE AN3, CPO gene tAG fusion with Kex site (KR)

<400>  12
atgtcgttcc gatctctact cgccctgagc ggcctcgtct gcacagggtt ggcaaatgtg       60

atttccaagc gcgcgacctt ggattcatgg ttgagcaacg aagcgaccgt ggctcgtact      120

gccatcctga ataacatcgg ggcggacggt gcttgggtgt cgggcgcgga ctctggcatt      180

gtcgttgcta gtcccagcac ggataacccg gactacttct acacctggac tcgcgactct      240

ggtctcgtcc tcaagaccct cgtcgatctc ttccgaaatg gagataccag tctcctctcc      300

accattgaga actacatctc cgcccaggca attgtccagg gtatcagtaa cccctctggt      360

gatctgtcca gcggcgctgg tctcggtgaa cccaagttca atgtcgatga gactgcctac      420

actggttctt ggggacggcc gcagcgagat ggtccggctc tgagagcaac tgctatgatc      480

ggcttcgggc agtggctgct tgacaatggc tacaccagca ccgcaacgga cattgtttgg      540

cccctcgtta ggaacgacct gtcgtatgtg gctcaatact ggaaccagac aggatatgat      600

ctctgggaag aagtcaatgg ctcgtctttc tttacgattg ctgtgcaaca ccgcgccctt      660

gtcgaaggta gtgccttcgc gacggccgtc ggctcgtcct gctcctggtg tgattctcag      720

gcacccgaaa ttctctgcta cctgcagtcc ttctggaccg gcagcttcat tctggccaac      780

ttcgatagca gccgttccgg caaggacgca aacaccctcc tgggaagcat ccacaccttt      840

gatcctgagg ccgcatgcga cgactccacc ttccagccct gctccccgcg cgcgctcgcc      900

aaccacaagg aggttgtaga ctctttccgc tcaatctata ccctcaacga tggtctcagt      960

gacagcgagg ctgttgcggt gggtcggtac cctgaggaca cgtactacaa cggcaacccg     1020

tggttcctgt gcaccttggc tgccgcagag cagttgtacg atgctctata ccagtgggac     1080

aagcaggggt cgttggaggt cacagatgtg tcgctggact tcttcaaggc actgtacagc     1140

gatgctgcta ctggcaccta ctcttcgtcc agttcgactt atagtagcat tgtagatgcc     1200

gtgaagactt tcgccgatgg cttcgtctct attgtggaaa ctcacgccgc aagcaacggc     1260

tccatgtccg agcaatacga caagtctgat ggcgagcagc tttccgctcg cgacctgacc     1320

tggtcttatg ctgctctgct gaccgccaac aaccgtcgta actccgtcgt gcctgcttct     1380

tggggcgaga cctctgccag cagcgtgccc ggcacctgtg cggccacatc tgccattggt     1440

acctacagca gtgtgactgt cacctcgtgg ccgagtatcg tggctactgg cggcaccact     1500

acgacggcta cccccactgg atccggcagc gtgacctcga ccagcaagac caccgcgact     1560

gctagcaagc gcttcctggg caagattgcc aagaaccccg aggccagcat gaacgtttcc     1620

cagatgatct cttactgggg ttacccctcc gaggagcaca aggtcaccac cgaggatggc     1680

tacatcctgg aggtcaaccg tatcccccac ggcaagaaga actccgagaa caccggccag     1740

cgtcccgttg tctttctaca acacggtctg cttggctctg ccaccaactg gatctccaac     1800

ctccccaaga actctcttgg tttcctcctt gccgatgctg gttacgatgt ctggctaggc     1860

aactcccgtg gtaacacctg ggctcaggag cacctctact actctcctga ctccgacgag     1920

ttctgggctt tctccttcga cgagatggcc gagtacgacc tccccagcac catcgacttc     1980

atcctccgcc gcactggcca ggagcagctg cactacgtcg gtcactccca gggtactacc     2040

atcggtttca ttgctttctc cacctccccc gagcttgctg agaagatcaa gaccttctac     2100

gctcttgctc ctgttgccac tgtcaagtac accaagtccc tcttcaacaa gctggctctg     2160

atccccgagt tcctcttcaa ggacctcttc ggtgacaaga tgttctaccc ccacaccttc     2220

ctggagcagt tcctgggtgt tgagatgtgc tctcgcgaaa ccctcgatgt cctttgcaag     2280

aacgccctct tcgccatcac cggtgttgac aacaagaact tcaacatgag ccgtctggat     2340

gtctacattg cccacaaccc tgctggtact tccgtccaga acaccctcca ctggcgccag     2400

gctgtcaagt ccggcaagtt ccaggcctac gactggggtt cccccgacca gaaccaggag     2460

cactacgacc agagcactcc tcccatctac aacctgaccg acatgaacgt ccccactgct     2520

gtctggtccg ccgacaacga cctccttgcc gacccccagg atgttgagaa cctcctcagc     2580

aagctcagca acctgatcta ccacaaggag atccccaact acaaccacct cgacttcatc     2640

tggggtgagg atgctcctca ggaagtctac aacgaaatag tatctctgat ggaagaggac     2700

aagaagtaaa                                                            2710


<210>  13
<211>  2710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE variant AN12, pI shift from 6.96 to 4.6

<400>  13
atgtcgttcc gatctctact cgccctgagc ggcctcgtct gcacagggtt ggcaaatgtg       60

atttccaagc gcgcgacctt ggattcatgg ttgagcaacg aagcgaccgt ggctcgtact      120

gccatcctga ataacatcgg ggcggacggt gcttgggtgt cgggcgcgga ctctggcatt      180

gtcgttgcta gtcccagcac ggataacccg gactacttct acacctggac tcgcgactct      240

ggtctcgtcc tcaagaccct cgtcgatctc ttccgaaatg gagataccag tctcctctcc      300

accattgaga actacatctc cgcccaggca attgtccagg gtatcagtaa cccctctggt      360

gatctgtcca gcggcgctgg tctcggtgaa cccaagttca atgtcgatga gactgcctac      420

actggttctt ggggacggcc gcagcgagat ggtccggctc tgagagcaac tgctatgatc      480

ggcttcgggc agtggctgct tgacaatggc tacaccagca ccgcaacgga cattgtttgg      540

cccctcgtta ggaacgacct gtcgtatgtg gctcaatact ggaaccagac aggatatgat      600

ctctgggaag aagtcaatgg ctcgtctttc tttacgattg ctgtgcaaca ccgcgccctt      660

gtcgaaggta gtgccttcgc gacggccgtc ggctcgtcct gctcctggtg tgattctcag      720

gcacccgaaa ttctctgcta cctgcagtcc ttctggaccg gcagcttcat tctggccaac      780

ttcgatagca gccgttccgg caaggacgca aacaccctcc tgggaagcat ccacaccttt      840

gatcctgagg ccgcatgcga cgactccacc ttccagccct gctccccgcg cgcgctcgcc      900

aaccacaagg aggttgtaga ctctttccgc tcaatctata ccctcaacga tggtctcagt      960

gacagcgagg ctgttgcggt gggtcggtac cctgaggaca cgtactacaa cggcaacccg     1020

tggttcctgt gcaccttggc tgccgcagag cagttgtacg atgctctata ccagtgggac     1080

aagcaggggt cgttggaggt cacagatgtg tcgctggact tcttcaaggc actgtacagc     1140

gatgctgcta ctggcaccta ctcttcgtcc agttcgactt atagtagcat tgtagatgcc     1200

gtgaagactt tcgccgatgg cttcgtctct attgtggaaa ctcacgccgc aagcaacggc     1260

tccatgtccg agcaatacga caagtctgat ggcgagcagc tttccgctcg cgacctgacc     1320

tggtcttatg ctgctctgct gaccgccaac aaccgtcgta actccgtcgt gcctgcttct     1380

tggggcgaga cctctgccag cagcgtgccc ggcacctgtg cggccacatc tgccattggt     1440

acctacagca gtgtgactgt cacctcgtgg ccgagtatcg tggctactgg cggcaccact     1500

acgacggcta cccccactgg atccggcagc gtgacctcga ccagcaagac caccgcgact     1560

gctagcaagc gcttcctggg caagattgcc aagaaccccg aggccagcat gaacgtttcc     1620

cagatgatct cctactgggg ttacccctcc gaggagtacg aggtcaccac tgaggatggc     1680

tacatcctgg aggtcaaccg tatcccccac ggcaagaaga actccgagaa cactggccag     1740

cgtcccgttg ttttcctgca gcacggtctg cttggttccg ccaccaactg gatctccaac     1800

ctccccaaca actcgctagg attcctcctt gccgatgccg gttacgatgt ctggctaggc     1860

aactcccgtg gtaacacctg ggctcgccgc aacctctact actctcctga ctccgttgag     1920

ttctgggctt tctccttcga cgagatggct gagtacgacc tccccagcac cattgacttc     1980

atcctggaaa agaccggcca ggagcagctg cactacgtcg gtcactccca gggtactacc     2040

atcggtttca ttgctttctc cacctccccc gagcttgctg agaagatcaa gaccttctac     2100

gctcttgctc ctgttgccac cgtcaagtac accaagtccc tcttcaacaa gcttgctctg     2160

atcccccagt ccctcttcaa ggacctcttc ggtgacaagg agttctaccc ccacaccttc     2220

ctggagcagt tcctggccac tgagatgtgc tctcgcgaaa ccctcgatgt cctctgcaag     2280

aacgccctct tcgccatcac cggtgttgac aacaagaact tcaacatgag ccgtctggat     2340

gtctaccttt ctcacaaccc tgctggcacc tccgtccaga acaccctcca ctggcgccag     2400

gctgtcaagt ccggcaagtt ccaggcctac gactggggtt ctcctgacca gaaccaggag     2460

cactacgacc agagcactcc tcccatctac aacctcaccg acatgaacgt ccccactgct     2520

gtctggtctg ccgacaacga cctccttgcc gacccccagg atgttgagaa cctcctcagc     2580

aagctcagca acctgatcta ccacaaggag atccccaact acaaccacct cgacttcatc     2640

tggggtgagg atgctcctca ggaagtctac aacgaaatag tatctctgat ggaagaagat     2700

aaaaagtaaa                                                            2710


<210>  14
<211>  902
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE variant AN12, pI shift from 6.96 to 4.6

<400>  14

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      


Leu Ala Asn Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser 
            20                  25                  30          


Asn Glu Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala 
        35                  40                  45              


Asp Gly Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser 
    50                  55                  60                  


Pro Ser Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser 
65                  70                  75                  80  


Gly Leu Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr 
                85                  90                  95      


Ser Leu Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val 
            100                 105                 110         


Gln Gly Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu 
        115                 120                 125             


Gly Glu Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp 
    130                 135                 140                 


Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile 
145                 150                 155                 160 


Gly Phe Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr 
                165                 170                 175     


Asp Ile Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln 
            180                 185                 190         


Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser 
        195                 200                 205             


Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser 
    210                 215                 220                 


Ala Phe Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln 
225                 230                 235                 240 


Ala Pro Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe 
                245                 250                 255     


Ile Leu Ala Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Ala Asn Thr 
            260                 265                 270         


Leu Leu Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp 
        275                 280                 285             


Ser Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu 
    290                 295                 300                 


Val Val Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser 
305                 310                 315                 320 


Asp Ser Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr 
                325                 330                 335     


Asn Gly Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu 
            340                 345                 350         


Tyr Asp Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr 
        355                 360                 365             


Asp Val Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Ala Thr 
    370                 375                 380                 


Gly Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala 
385                 390                 395                 400 


Val Lys Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala 
                405                 410                 415     


Ala Ser Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu 
            420                 425                 430         


Gln Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr 
        435                 440                 445             


Ala Asn Asn Arg Arg Asn Ser Val Val Pro Ala Ser Trp Gly Glu Thr 
    450                 455                 460                 


Ser Ala Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly 
465                 470                 475                 480 


Thr Tyr Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr 
                485                 490                 495     


Gly Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr 
            500                 505                 510         


Ser Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Arg Phe Leu Gly Lys 
        515                 520                 525             


Ile Ala Lys Asn Pro Glu Ala Ser Met Asn Val Ser Gln Met Ile Ser 
    530                 535                 540                 


Tyr Trp Gly Tyr Pro Ser Glu Glu Tyr Glu Val Thr Thr Glu Asp Gly 
545                 550                 555                 560 


Tyr Ile Leu Glu Val Asn Arg Ile Pro His Gly Lys Lys Asn Ser Glu 
                565                 570                 575     


Asn Thr Gly Gln Arg Pro Val Val Phe Leu Gln His Gly Leu Leu Gly 
            580                 585                 590         


Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro Asn Asn Ser Leu Gly Phe 
        595                 600                 605             


Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly Asn Ser Arg Gly 
    610                 615                 620                 


Asn Thr Trp Ala Arg Arg Asn Leu Tyr Tyr Ser Pro Asp Ser Val Glu 
625                 630                 635                 640 


Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Glu Tyr Asp Leu Pro Ser 
                645                 650                 655     


Thr Ile Asp Phe Ile Leu Glu Lys Thr Gly Gln Glu Gln Leu His Tyr 
            660                 665                 670         


Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe Ser Thr 
        675                 680                 685             


Ser Pro Glu Leu Ala Glu Lys Ile Lys Thr Phe Tyr Ala Leu Ala Pro 
    690                 695                 700                 


Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn Lys Leu Ala Leu 
705                 710                 715                 720 


Ile Pro Gln Ser Leu Phe Lys Asp Leu Phe Gly Asp Lys Glu Phe Tyr 
                725                 730                 735     


Pro His Thr Phe Leu Glu Gln Phe Leu Ala Thr Glu Met Cys Ser Arg 
            740                 745                 750         


Glu Thr Leu Asp Val Leu Cys Lys Asn Ala Leu Phe Ala Ile Thr Gly 
        755                 760                 765             


Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp Val Tyr Leu Ser 
    770                 775                 780                 


His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu His Trp Arg Gln 
785                 790                 795                 800 


Ala Val Lys Ser Gly Lys Phe Gln Ala Tyr Asp Trp Gly Ser Pro Asp 
                805                 810                 815     


Gln Asn Gln Glu His Tyr Asp Gln Ser Thr Pro Pro Ile Tyr Asn Leu 
            820                 825                 830         


Thr Asp Met Asn Val Pro Thr Ala Val Trp Ser Ala Asp Asn Asp Leu 
        835                 840                 845             


Leu Ala Asp Pro Gln Asp Val Glu Asn Leu Leu Ser Lys Leu Ser Asn 
    850                 855                 860                 


Leu Ile Tyr His Lys Glu Ile Pro Asn Tyr Asn His Leu Asp Phe Ile 
865                 870                 875                 880 


Trp Gly Glu Asp Ala Pro Gln Glu Val Tyr Asn Glu Ile Val Ser Leu 
                885                 890                 895     


Met Glu Glu Asp Lys Lys 
            900         


<210>  15
<211>  2710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGE variant AN13, pI shift from 6.96 to 4.88

<400>  15
atgtcgttcc gatctctact cgccctgagc ggcctcgtct gcacagggtt ggcaaatgtg       60

atttccaagc gcgcgacctt ggattcatgg ttgagcaacg aagcgaccgt ggctcgtact      120

gccatcctga ataacatcgg ggcggacggt gcttgggtgt cgggcgcgga ctctggcatt      180

gtcgttgcta gtcccagcac ggataacccg gactacttct acacctggac tcgcgactct      240

ggtctcgtcc tcaagaccct cgtcgatctc ttccgaaatg gagataccag tctcctctcc      300

accattgaga actacatctc cgcccaggca attgtccagg gtatcagtaa cccctctggt      360

gatctgtcca gcggcgctgg tctcggtgaa cccaagttca atgtcgatga gactgcctac      420

actggttctt ggggacggcc gcagcgagat ggtccggctc tgagagcaac tgctatgatc      480

ggcttcgggc agtggctgct tgacaatggc tacaccagca ccgcaacgga cattgtttgg      540

cccctcgtta ggaacgacct gtcgtatgtg gctcaatact ggaaccagac aggatatgat      600

ctctgggaag aagtcaatgg ctcgtctttc tttacgattg ctgtgcaaca ccgcgccctt      660

gtcgaaggta gtgccttcgc gacggccgtc ggctcgtcct gctcctggtg tgattctcag      720

gcacccgaaa ttctctgcta cctgcagtcc ttctggaccg gcagcttcat tctggccaac      780

ttcgatagca gccgttccgg caaggacgca aacaccctcc tgggaagcat ccacaccttt      840

gatcctgagg ccgcatgcga cgactccacc ttccagccct gctccccgcg cgcgctcgcc      900

aaccacaagg aggttgtaga ctctttccgc tcaatctata ccctcaacga tggtctcagt      960

gacagcgagg ctgttgcggt gggtcggtac cctgaggaca cgtactacaa cggcaacccg     1020

tggttcctgt gcaccttggc tgccgcagag cagttgtacg atgctctata ccagtgggac     1080

aagcaggggt cgttggaggt cacagatgtg tcgctggact tcttcaaggc actgtacagc     1140

gatgctgcta ctggcaccta ctcttcgtcc agttcgactt atagtagcat tgtagatgcc     1200

gtgaagactt tcgccgatgg cttcgtctct attgtggaaa ctcacgccgc aagcaacggc     1260

tccatgtccg agcaatacga caagtctgat ggcgagcagc tttccgctcg cgacctgacc     1320

tggtcttatg ctgctctgct gaccgccaac aaccgtcgta actccgtcgt gcctgcttct     1380

tggggcgaga cctctgccag cagcgtgccc ggcacctgtg cggccacatc tgccattggt     1440

acctacagca gtgtgactgt cacctcgtgg ccgagtatcg tggctactgg cggcaccact     1500

acgacggcta cccccactgg atccggcagc gtgacctcga ccagcaagac caccgcgact     1560

gctagcaagc gcctcttcgg caagctccac cccaaccccg aggccaacat gaacatctcc     1620

cagatcatct cctactgggg ttacccctcc gaggagtacg aggttgtcac cgaggatggc     1680

tacatcctcg aggtcaaccg tatcccccac ggcaagaaca acgccaacaa cactggccag     1740

cgtcccgttg ttttcctgca gcacggtctg cttggctctg ccagcaactg gatctccaac     1800

ctccccaaca actctcttgg tttcctcctt gccgatgctg gttacgatgt ctggctaggc     1860

aactcccgtg gtaacacctg gtcccgcaag cacaagaccc tcagccctga cagcgttgag     1920

ttctgggctt tctccttcga cgagatggcc aagtacgacc tccccgccac cattgacttc     1980

atcctggaaa agaccggcca ggagcagctc tactacgtcg gtcactccca gggtactacc     2040

attggtttca ttgctttctc caccaacccc gagcttgctg agaagatcaa gaccttctac     2100

gcccttgctc ctgttgccac cgtcaagtac accaagtccc tcttcaacaa gctggctctg     2160

atccccgact ccctcttcaa ggtcctcttc ggtgacaagg agttctaccc ccacaccttc     2220

ctggagcagt tcctggccac tgagatgtgc tctcgcgaaa ccctcgacct cctttgctcc     2280

aacgccctct tcgccatcac cggtgttgac aacaagaact tcaacatgag ccgtctggat     2340

gtctacctct cccacaaccc tgctggtact tccgtccaga acaccctcca ctggcgccag     2400

gctgtcaagt ccggcaagtt ccaggctttc gactggggtt ctcctgacca gaaccaggag     2460

cactacaacc agagcactcc tcctctgtac aacctgaccg acatgaacgt ccccactgct     2520

gtctggtccg gtggtcagga tcttctggcc gacccccagg atgttgacct cctcctcccc     2580

aagatcacca acctgatcta ccacaaggag atccccaact acaaccacct cgacttcatc     2640

tgggccatgg atgctcctca ggaagtctac aacgaaatag tatctctgat ggaagaggac     2700

aagaagtaaa                                                            2710


<210>  16
<211>  902
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGE variant AN13, pI shift from 6.96 to 4.88

<400>  16

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      


Leu Ala Asn Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser 
            20                  25                  30          


Asn Glu Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala 
        35                  40                  45              


Asp Gly Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser 
    50                  55                  60                  


Pro Ser Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser 
65                  70                  75                  80  


Gly Leu Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr 
                85                  90                  95      


Ser Leu Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val 
            100                 105                 110         


Gln Gly Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu 
        115                 120                 125             


Gly Glu Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp 
    130                 135                 140                 


Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile 
145                 150                 155                 160 


Gly Phe Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr 
                165                 170                 175     


Asp Ile Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln 
            180                 185                 190         


Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser 
        195                 200                 205             


Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser 
    210                 215                 220                 


Ala Phe Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln 
225                 230                 235                 240 


Ala Pro Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe 
                245                 250                 255     


Ile Leu Ala Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Ala Asn Thr 
            260                 265                 270         


Leu Leu Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp 
        275                 280                 285             


Ser Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu 
    290                 295                 300                 


Val Val Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser 
305                 310                 315                 320 


Asp Ser Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr 
                325                 330                 335     


Asn Gly Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu 
            340                 345                 350         


Tyr Asp Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr 
        355                 360                 365             


Asp Val Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Ala Thr 
    370                 375                 380                 


Gly Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala 
385                 390                 395                 400 


Val Lys Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala 
                405                 410                 415     


Ala Ser Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu 
            420                 425                 430         


Gln Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr 
        435                 440                 445             


Ala Asn Asn Arg Arg Asn Ser Val Val Pro Ala Ser Trp Gly Glu Thr 
    450                 455                 460                 


Ser Ala Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly 
465                 470                 475                 480 


Thr Tyr Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr 
                485                 490                 495     


Gly Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr 
            500                 505                 510         


Ser Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Arg Leu Phe Gly Lys 
        515                 520                 525             


Leu His Pro Asn Pro Glu Ala Asn Met Asn Ile Ser Gln Ile Ile Ser 
    530                 535                 540                 


Tyr Trp Gly Tyr Pro Ser Glu Glu Tyr Glu Val Val Thr Glu Asp Gly 
545                 550                 555                 560 


Tyr Ile Leu Glu Val Asn Arg Ile Pro His Gly Lys Asn Asn Ala Asn 
                565                 570                 575     


Asn Thr Gly Gln Arg Pro Val Val Phe Leu Gln His Gly Leu Leu Gly 
            580                 585                 590         


Ser Ala Ser Asn Trp Ile Ser Asn Leu Pro Asn Asn Ser Leu Gly Phe 
        595                 600                 605             


Leu Leu Ala Asp Ala Gly Tyr Asp Val Trp Leu Gly Asn Ser Arg Gly 
    610                 615                 620                 


Asn Thr Trp Ser Arg Lys His Lys Thr Leu Ser Pro Asp Ser Val Glu 
625                 630                 635                 640 


Phe Trp Ala Phe Ser Phe Asp Glu Met Ala Lys Tyr Asp Leu Pro Ala 
                645                 650                 655     


Thr Ile Asp Phe Ile Leu Glu Lys Thr Gly Gln Glu Gln Leu Tyr Tyr 
            660                 665                 670         


Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe Ser Thr 
        675                 680                 685             


Asn Pro Glu Leu Ala Glu Lys Ile Lys Thr Phe Tyr Ala Leu Ala Pro 
    690                 695                 700                 


Val Ala Thr Val Lys Tyr Thr Lys Ser Leu Phe Asn Lys Leu Ala Leu 
705                 710                 715                 720 


Ile Pro Asp Ser Leu Phe Lys Val Leu Phe Gly Asp Lys Glu Phe Tyr 
                725                 730                 735     


Pro His Thr Phe Leu Glu Gln Phe Leu Ala Thr Glu Met Cys Ser Arg 
            740                 745                 750         


Glu Thr Leu Asp Leu Leu Cys Ser Asn Ala Leu Phe Ala Ile Thr Gly 
        755                 760                 765             


Val Asp Asn Lys Asn Phe Asn Met Ser Arg Leu Asp Val Tyr Leu Ser 
    770                 775                 780                 


His Asn Pro Ala Gly Thr Ser Val Gln Asn Thr Leu His Trp Arg Gln 
785                 790                 795                 800 


Ala Val Lys Ser Gly Lys Phe Gln Ala Phe Asp Trp Gly Ser Pro Asp 
                805                 810                 815     


Gln Asn Gln Glu His Tyr Asn Gln Ser Thr Pro Pro Leu Tyr Asn Leu 
            820                 825                 830         


Thr Asp Met Asn Val Pro Thr Ala Val Trp Ser Gly Gly Gln Asp Leu 
        835                 840                 845             


Leu Ala Asp Pro Gln Asp Val Asp Leu Leu Leu Pro Lys Ile Thr Asn 
    850                 855                 860                 


Leu Ile Tyr His Lys Glu Ile Pro Asn Tyr Asn His Leu Asp Phe Ile 
865                 870                 875                 880 


Trp Ala Met Asp Ala Pro Gln Glu Val Tyr Asn Glu Ile Val Ser Leu 
                885                 890                 895     


Met Glu Glu Asp Lys Lys 
            900         


<210>  17
<211>  1342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chitinase (ZDU) wild-type

<400>  17
atgaagctct tctccatcct cctcgtcgct cttgtatctc tggtcagcgg tgctcaccac       60

caccgtcacc gtcacatgaa cgaccctgct cctgcttctt tccccggtcc ccaccaccag      120

caccacggta tccctcctca caagctggtt gctcgcaaca cttctgctga gtacaagtcc      180

attgcctact tcgtcaactg ggccatctac ggccgcaacc acaaccccca ggacatcccc      240

attgacaagc tcacccacat cctgtacgct ttcgccaacg tccgtgccaa cggcgaggtc      300

tacctctccg acccctggtc cgacattgac aagcgcttcc ccggtgacag ctggtccgac      360

accggcaaca acgtctacgg ctgtgtcaag cagctcaacc tcctcaagca gaagaaccgc      420

aacctcaagg tcctcctctc catcggtggc tggacctact cttccaactt cgtcaccccc      480

gccagcactg accagggtcg caagaccttc gcctcttctg ctgtcaagct ccttgccgac      540

cttggtttcg acggtctgga cattgactgg gaataccccg ccaacgaggc tcaggccacc      600

gacatggtcc tcctcctccg tgagatccgt gaggagcttg acgagtacgg ccgtgagcac      660

ggcaacggca cccacttcct gctctccatt gccacctccg ccggtccctc caagtacaac      720

accctgcaca tctcctccat gaacctctac ctcgacttct ggaacctgat ggcctacgac      780

tacgctggca gctgggatgc cactgctggc caccaggcca acctgtaccc cctccgtgat      840

gaccccgtca gcactccctt caacaccgac caggccatct ccgcctacct cactgctggt      900

gttcctgctc acaagctgat cctgggtatg cccctctacg gccgtgcttt caccaacacc      960

gacggtcccg gcaagccctt caacggtgtt ggtcagggca gctgggagaa cggtgtctgg     1020

gactacaagg cccttcctcc tgctggtgcc tccgtcagcg agctgcgctc catcggtgcc     1080

agctactcct acgatgctgg caagaaaacc atgatatctt acgacactcc tgctgttgct     1140

cgccagaagg ccgactacat ccgctccaag ggtcttggtg gtgccatgtg gtgggaaacc     1200

tccggtgaca aggtcggtgt tgagtccctg atctccaccg ttgttgactc tcttggtggt     1260

atcggtgctc ttgacaagtc cgtcaaccac ctggaatacc ccgagtccca gtacgacaac     1320

gtcaagaagg gtttccacta aa                                              1342


<210>  18
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chitinase (ZDU) wild-type

<400>  18

Met Lys Leu Phe Ser Ile Leu Leu Val Ala Leu Val Ser Leu Val Ser 
1               5                   10                  15      


Gly Ala His His His Arg His Arg His Met Asn Asp Pro Ala Pro Ala 
            20                  25                  30          


Ser Phe Pro Gly Pro His His Gln His His Gly Ile Pro Pro His Lys 
        35                  40                  45              


Leu Val Ala Arg Asn Thr Ser Ala Glu Tyr Lys Ser Ile Ala Tyr Phe 
    50                  55                  60                  


Val Asn Trp Ala Ile Tyr Gly Arg Asn His Asn Pro Gln Asp Ile Pro 
65                  70                  75                  80  


Ile Asp Lys Leu Thr His Ile Leu Tyr Ala Phe Ala Asn Val Arg Ala 
                85                  90                  95      


Asn Gly Glu Val Tyr Leu Ser Asp Pro Trp Ser Asp Ile Asp Lys Arg 
            100                 105                 110         


Phe Pro Gly Asp Ser Trp Ser Asp Thr Gly Asn Asn Val Tyr Gly Cys 
        115                 120                 125             


Val Lys Gln Leu Asn Leu Leu Lys Gln Lys Asn Arg Asn Leu Lys Val 
    130                 135                 140                 


Leu Leu Ser Ile Gly Gly Trp Thr Tyr Ser Ser Asn Phe Val Thr Pro 
145                 150                 155                 160 


Ala Ser Thr Asp Gln Gly Arg Lys Thr Phe Ala Ser Ser Ala Val Lys 
                165                 170                 175     


Leu Leu Ala Asp Leu Gly Phe Asp Gly Leu Asp Ile Asp Trp Glu Tyr 
            180                 185                 190         


Pro Ala Asn Glu Ala Gln Ala Thr Asp Met Val Leu Leu Leu Arg Glu 
        195                 200                 205             


Ile Arg Glu Glu Leu Asp Glu Tyr Gly Arg Glu His Gly Asn Gly Thr 
    210                 215                 220                 


His Phe Leu Leu Ser Ile Ala Thr Ser Ala Gly Pro Ser Lys Tyr Asn 
225                 230                 235                 240 


Thr Leu His Ile Ser Ser Met Asn Leu Tyr Leu Asp Phe Trp Asn Leu 
                245                 250                 255     


Met Ala Tyr Asp Tyr Ala Gly Ser Trp Asp Ala Thr Ala Gly His Gln 
            260                 265                 270         


Ala Asn Leu Tyr Pro Leu Arg Asp Asp Pro Val Ser Thr Pro Phe Asn 
        275                 280                 285             


Thr Asp Gln Ala Ile Ser Ala Tyr Leu Thr Ala Gly Val Pro Ala His 
    290                 295                 300                 


Lys Leu Ile Leu Gly Met Pro Leu Tyr Gly Arg Ala Phe Thr Asn Thr 
305                 310                 315                 320 


Asp Gly Pro Gly Lys Pro Phe Asn Gly Val Gly Gln Gly Ser Trp Glu 
                325                 330                 335     


Asn Gly Val Trp Asp Tyr Lys Ala Leu Pro Pro Ala Gly Ala Ser Val 
            340                 345                 350         


Ser Glu Leu Arg Ser Ile Gly Ala Ser Tyr Ser Tyr Asp Ala Gly Lys 
        355                 360                 365             


Lys Thr Met Ile Ser Tyr Asp Thr Pro Ala Val Ala Arg Gln Lys Ala 
    370                 375                 380                 


Asp Tyr Ile Arg Ser Lys Gly Leu Gly Gly Ala Met Trp Trp Glu Thr 
385                 390                 395                 400 


Ser Gly Asp Lys Val Gly Val Glu Ser Leu Ile Ser Thr Val Val Asp 
                405                 410                 415     


Ser Leu Gly Gly Ile Gly Ala Leu Asp Lys Ser Val Asn His Leu Glu 
            420                 425                 430         


Tyr Pro Glu Ser Gln Tyr Asp Asn Val Lys Lys Gly Phe His 
        435                 440                 445     


<210>  19
<211>  1342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chitinase variant ZDU-6

<400>  19
atgaagctct tctccatcct gctggtcgct cttgtctctt tagtgtctgg cgctcaccac       60

caccgtcacc gtcacatgaa cgaccctgct cctgcctcct tccccggtcc ccaccaccag      120

caccacggta tccctcctca caagcttgtt gctcgcaaca cttctgctga gtaccgctcg      180

gtggcctact tcgtcaactg ggccatctac ggccgcaacc acaaccccca ggatcttcct      240

gctgagaagc tcacccacgt cctctacgct ttcgccaacg tccgtcccac tggtgaagtc      300

tacctcaccg acacctggtc ggataccgac aagcactacc ccaccgacag ctggtccgac      360

actggtaaca acgtctacgg ctgcgtcaag cagctcttcc tgctcaagca gcagaaccgc      420

aacctcaagg tcctcctctc catcggtggc tggacctact cttccaactt cgctcagccc      480

gcctccactg atgccggtcg caagaccttc gcctccactg ccgtcaagct cctccaggat      540

cttggattcg atggcttgga tattgactgg gaataccccg agaacgacca gcaggcctcc      600

gacttcgtcc tcctcctcaa ggagatccgt gaggagctgg acaactactc tgctgctcac      660

gccaacggcc agcacttctt gttgactgtt gcctcccccg ctggtcccca gaactacaac      720

aagctccgtc tgcaggagat gaccccctac cttgacttct ggaacctgat ggcctacgac      780

tacgctggca gctgggattc tgttgctggc caccaggcca acctgtaccc cagcacctcc      840

aaccccgcca gcactccttt caacaccgac caggctgttg actactacat ctccgctggt      900

gttcctgcct ccaagattgt cctgggcatg cccttgtacg gccgtgcttt caccaacacc      960

gatggccctg gtaccccctt caacggtgtc ggccagggca gctgggagaa cggtgtgtgg     1020

gattacaagg cccttcctca ggctggtgcc accgaacaca ccgatgactc gattggtgcc     1080

tcctacagct acgaccccag cactcgtacc atgatctcct acgacacccc cgccgttgct     1140

gagatgaagg ctgagtacat ccgctccaag ggtctgggtg gtgccatgtg gtgggagact     1200

tctgccgaca agaccggtga ggattcgctg atcaccaccg tcgtcgaggg tgttggtggt     1260

gttggtgctc ttgaccagtc ccagaaccac ctggaatacc ccgagagcca gtacgacaac     1320

ctgcgcaacg gattccacta aa                                              1342


<210>  20
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chitinase variant ZDU-6

<400>  20

Met Lys Leu Phe Ser Ile Leu Leu Val Ala Leu Val Ser Leu Val Ser 
1               5                   10                  15      


Gly Ala His His His Arg His Arg His Met Asn Asp Pro Ala Pro Ala 
            20                  25                  30          


Ser Phe Pro Gly Pro His His Gln His His Gly Ile Pro Pro His Lys 
        35                  40                  45              


Leu Val Ala Arg Asn Thr Ser Ala Glu Tyr Arg Ser Val Ala Tyr Phe 
    50                  55                  60                  


Val Asn Trp Ala Ile Tyr Gly Arg Asn His Asn Pro Gln Asp Leu Pro 
65                  70                  75                  80  


Ala Glu Lys Leu Thr His Val Leu Tyr Ala Phe Ala Asn Val Arg Pro 
                85                  90                  95      


Thr Gly Glu Val Tyr Leu Thr Asp Thr Trp Ser Asp Thr Asp Lys His 
            100                 105                 110         


Tyr Pro Thr Asp Ser Trp Ser Asp Thr Gly Asn Asn Val Tyr Gly Cys 
        115                 120                 125             


Val Lys Gln Leu Phe Leu Leu Lys Gln Gln Asn Arg Asn Leu Lys Val 
    130                 135                 140                 


Leu Leu Ser Ile Gly Gly Trp Thr Tyr Ser Ser Asn Phe Ala Gln Pro 
145                 150                 155                 160 


Ala Ser Thr Asp Ala Gly Arg Lys Thr Phe Ala Ser Thr Ala Val Lys 
                165                 170                 175     


Leu Leu Gln Asp Leu Gly Phe Asp Gly Leu Asp Ile Asp Trp Glu Tyr 
            180                 185                 190         


Pro Glu Asn Asp Gln Gln Ala Ser Asp Phe Val Leu Leu Leu Lys Glu 
        195                 200                 205             


Ile Arg Glu Glu Leu Asp Asn Tyr Ser Ala Ala His Ala Asn Gly Gln 
    210                 215                 220                 


His Phe Leu Leu Thr Val Ala Ser Pro Ala Gly Pro Gln Asn Tyr Asn 
225                 230                 235                 240 


Lys Leu Arg Leu Gln Glu Met Thr Pro Tyr Leu Asp Phe Trp Asn Leu 
                245                 250                 255     


Met Ala Tyr Asp Tyr Ala Gly Ser Trp Asp Ser Val Ala Gly His Gln 
            260                 265                 270         


Ala Asn Leu Tyr Pro Ser Thr Ser Asn Pro Ala Ser Thr Pro Phe Asn 
        275                 280                 285             


Thr Asp Gln Ala Val Asp Tyr Tyr Ile Ser Ala Gly Val Pro Ala Ser 
    290                 295                 300                 


Lys Ile Val Leu Gly Met Pro Leu Tyr Gly Arg Ala Phe Thr Asn Thr 
305                 310                 315                 320 


Asp Gly Pro Gly Thr Pro Phe Asn Gly Val Gly Gln Gly Ser Trp Glu 
                325                 330                 335     


Asn Gly Val Trp Asp Tyr Lys Ala Leu Pro Gln Ala Gly Ala Thr Glu 
            340                 345                 350         


His Thr Asp Asp Ser Ile Gly Ala Ser Tyr Ser Tyr Asp Pro Ser Thr 
        355                 360                 365             


Arg Thr Met Ile Ser Tyr Asp Thr Pro Ala Val Ala Glu Met Lys Ala 
    370                 375                 380                 


Glu Tyr Ile Arg Ser Lys Gly Leu Gly Gly Ala Met Trp Trp Glu Thr 
385                 390                 395                 400 


Ser Ala Asp Lys Thr Gly Glu Asp Ser Leu Ile Thr Thr Val Val Glu 
                405                 410                 415     


Gly Val Gly Gly Val Gly Ala Leu Asp Gln Ser Gln Asn His Leu Glu 
            420                 425                 430         


Tyr Pro Glu Ser Gln Tyr Asp Asn Leu Arg Asn Gly Phe His 
        435                 440                 445     


<210>  21
<211>  1285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chitinase variant ZDU-7

<400>  21
atgaagctct tctccatcct gctggtcgct cttgtctctt tagtgtctgg cgctgccatc       60

tggaaccgtg atgctcctag cactgatctg gaggctcgtg ccaacgccgg ttaccgctct      120

gttgcctact tcgtcaactg ggccatctac ggccgtaacc acaaccccca ggatcttcct      180

gctgagaagc tgacccacgt cctctacgct ttcgccaacg tccgtcccga gactggcgag      240

gtctacttga ctgacacctg gtccgacacc gacaagcact accccaccga cagctggtcc      300

gacactggca acaacgtcta cggcaacgtc aagcagctct tcctgctcaa gcagcagaac      360

cgcaacctga aggtcctcct ctccatcggt ggctggacct actctcccaa cttcgctcag      420

cctgccagca ctgatgctgg ccgcaagacc ttcgccagca ctgctgtcaa gctcctccag      480

gatcttggat tcgatggtct tgacattgac tgggaatacc ccgccaacga ccagcaggcc      540

tccgacttcg tcctcctcct caaggagatc cgtgaggagc tcgacaacta ctctgctgct      600

cacgctcctg gataccactt cctgctgacc gttgcctccc ccgccggtcc tcagaactac      660

aacaagctcc gcctccagga gatgaccccc taccttgact tctggaactt gatggcctac      720

gactacgctg gcagctggga ttctgttgct ggtcaccagg ccaacctgta cccctccacc      780

tcgaaccccg cctctactcc cttcaacacc gaccaggctg ttgactacta catctccgct      840

ggtgttcctg cctccaagat tgtccttgga atgcctctgt acggccgtgc tttcaccaac      900

accgatggcc ctggtacccc cttcaacggt gtcggccagg gcagctggga gaacggtgtc      960

tgggattaca aggcccttcc ccaggctggt gccactgagc acaccgatga ctccatcggt     1020

gcctcctact cctacgaccc cagcactcgt accatgatct cctacgacac ccccgccgtt     1080

gctgagatga aggctgaata catccgctcc aagggcttgg gtggtgccat gtggtgggaa     1140

acctctgccg acaagaccgg tgaggactct ctgatcacca ccgttgttga gggtgttggt     1200

ggtgttggtg ctctggatca gtcccagaac cacctggaat accccgagtc ccagtacgac     1260

aacctgcgca acggcttcca ctaaa                                           1285


<210>  22
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chitinase variant ZDU-7

<400>  22

Met Lys Leu Phe Ser Ile Leu Leu Val Ala Leu Val Ser Leu Val Ser 
1               5                   10                  15      


Gly Ala Ala Ile Trp Asn Arg Asp Ala Pro Ser Thr Asp Leu Glu Ala 
            20                  25                  30          


Arg Ala Asn Ala Gly Tyr Arg Ser Val Ala Tyr Phe Val Asn Trp Ala 
        35                  40                  45              


Ile Tyr Gly Arg Asn His Asn Pro Gln Asp Leu Pro Ala Glu Lys Leu 
    50                  55                  60                  


Thr His Val Leu Tyr Ala Phe Ala Asn Val Arg Pro Glu Thr Gly Glu 
65                  70                  75                  80  


Val Tyr Leu Thr Asp Thr Trp Ser Asp Thr Asp Lys His Tyr Pro Thr 
                85                  90                  95      


Asp Ser Trp Ser Asp Thr Gly Asn Asn Val Tyr Gly Asn Val Lys Gln 
            100                 105                 110         


Leu Phe Leu Leu Lys Gln Gln Asn Arg Asn Leu Lys Val Leu Leu Ser 
        115                 120                 125             


Ile Gly Gly Trp Thr Tyr Ser Pro Asn Phe Ala Gln Pro Ala Ser Thr 
    130                 135                 140                 


Asp Ala Gly Arg Lys Thr Phe Ala Ser Thr Ala Val Lys Leu Leu Gln 
145                 150                 155                 160 


Asp Leu Gly Phe Asp Gly Leu Asp Ile Asp Trp Glu Tyr Pro Ala Asn 
                165                 170                 175     


Asp Gln Gln Ala Ser Asp Phe Val Leu Leu Leu Lys Glu Ile Arg Glu 
            180                 185                 190         


Glu Leu Asp Asn Tyr Ser Ala Ala His Ala Pro Gly Tyr His Phe Leu 
        195                 200                 205             


Leu Thr Val Ala Ser Pro Ala Gly Pro Gln Asn Tyr Asn Lys Leu Arg 
    210                 215                 220                 


Leu Gln Glu Met Thr Pro Tyr Leu Asp Phe Trp Asn Leu Met Ala Tyr 
225                 230                 235                 240 


Asp Tyr Ala Gly Ser Trp Asp Ser Val Ala Gly His Gln Ala Asn Leu 
                245                 250                 255     


Tyr Pro Ser Thr Ser Asn Pro Ala Ser Thr Pro Phe Asn Thr Asp Gln 
            260                 265                 270         


Ala Val Asp Tyr Tyr Ile Ser Ala Gly Val Pro Ala Ser Lys Ile Val 
        275                 280                 285             


Leu Gly Met Pro Leu Tyr Gly Arg Ala Phe Thr Asn Thr Asp Gly Pro 
    290                 295                 300                 


Gly Thr Pro Phe Asn Gly Val Gly Gln Gly Ser Trp Glu Asn Gly Val 
305                 310                 315                 320 


Trp Asp Tyr Lys Ala Leu Pro Gln Ala Gly Ala Thr Glu His Thr Asp 
                325                 330                 335     


Asp Ser Ile Gly Ala Ser Tyr Ser Tyr Asp Pro Ser Thr Arg Thr Met 
            340                 345                 350         


Ile Ser Tyr Asp Thr Pro Ala Val Ala Glu Met Lys Ala Glu Tyr Ile 
        355                 360                 365             


Arg Ser Lys Gly Leu Gly Gly Ala Met Trp Trp Glu Thr Ser Ala Asp 
    370                 375                 380                 


Lys Thr Gly Glu Asp Ser Leu Ile Thr Thr Val Val Glu Gly Val Gly 
385                 390                 395                 400 


Gly Val Gly Ala Leu Asp Gln Ser Gln Asn His Leu Glu Tyr Pro Glu 
                405                 410                 415     


Ser Gln Tyr Asp Asn Leu Arg Asn Gly Phe His 
            420                 425         


<210>  23
<211>  1525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-glucosidase wild-type ZTB-WT

<400>  23
atgtccttcc gctctcttct ggctctgtcc ggtctggtct gcaccggtct ggctatgacc       60

accactgaga ctcctaccac caccaccacc tccaccctcc ctcctgactt cctctggggt      120

ttcgccactg cctcctacca gattgagggt gctttcgacg aggatggccg tctgccctcc      180

atctgggata ccttctccaa gacccccggc aaggttgagg atggcaccaa cggtgatgtt      240

gcttgcgaca gctaccaccg cactggtgag gacattgaga tcctcaagaa gtacggtgcc      300

aagatctacc gcttctctct gagctggccc cgtatcattc ctcttggtgg tcgcaacgac      360

cccatcaacg agaagggcct ccagttctac tccaagttcc tggatgacct ccacgctgct      420

ggtatcgagc ccttcgtcac cctattccac tgggatcttc ctgacgagct gatgaagcgt      480

tacggtggta tgctcaacaa ggaagaattt gtcgccgact acgccaacta cgcccgtgtt      540

gttttcaacg cccttggcag caaggtcaag cactggatca ccttcaacga gccctggtgc      600

tcctccgtcc ttggtcacaa cactggcaag cacgctcctg gtcgtacctc cgaccgtacc      660

aagtcccccg agggtgatgg cacccgtgag ccctggattg tcggtcacaa cctccttgtt      720

gctcacggta ctgttgttga catctaccgc cgtgagttca aggagaagca gggtggtgag      780

atcggtatca ccctcaacgg tgactgggct gagccctggg accccgagaa ccccgccgat      840

gttgaggctt gcgaccgcaa gatcgagttc gccatctcct ggttcgccga ccccatctac      900

cacggcaagt accccgacag catggtcaag cagcttggtg accgtctgcc caagttcacc      960

cccgaggaga ttgctttcgt ccacggcagc aacgacttct acggcatgaa ccactactgc     1020

gagaactaca tccgcaaccg cactggtgag cctgaccccg aggacattgc tggcaacctg     1080

gacatcctga tggaggacaa gaacggcaac cccatcggtc ccgagactca gtgcgaatgg     1140

ctacgcccct tccccctagg attccgcaag ctcctcaagt ggctagcgga ccgctacaac     1200

aaccccaaga tctacgtgac cgagaacggc acctccgtca agggtgagtc cgacaagcct     1260

ctggaagaag tcctcaacga cgagttccgt gtccagtact accgtgacta catcggtgcc     1320

atggtcgatg ccgttgctca ggatggtgtc aacgtcaagg cctacatggc ctggtccctc     1380

ctcgacaact tcgaatggtc cgagggctac cgctctcgct tcggtgtcac ctacgtcgac     1440

tacaagaacg gccagaagcg tatccccaag aagtctgctc tggtcattgg tgagctgttc     1500

aacaagtaca tccgcaagga gtaaa                                           1525


<210>  24
<211>  507
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Beta-glucosidase wild-type ZTB-WT

<400>  24

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      


Leu Ala Met Thr Thr Thr Glu Thr Pro Thr Thr Thr Thr Thr Ser Thr 
            20                  25                  30          


Leu Pro Pro Asp Phe Leu Trp Gly Phe Ala Thr Ala Ser Tyr Gln Ile 
        35                  40                  45              


Glu Gly Ala Phe Asp Glu Asp Gly Arg Leu Pro Ser Ile Trp Asp Thr 
    50                  55                  60                  


Phe Ser Lys Thr Pro Gly Lys Val Glu Asp Gly Thr Asn Gly Asp Val 
65                  70                  75                  80  


Ala Cys Asp Ser Tyr His Arg Thr Gly Glu Asp Ile Glu Ile Leu Lys 
                85                  90                  95      


Lys Tyr Gly Ala Lys Ile Tyr Arg Phe Ser Leu Ser Trp Pro Arg Ile 
            100                 105                 110         


Ile Pro Leu Gly Gly Arg Asn Asp Pro Ile Asn Glu Lys Gly Leu Gln 
        115                 120                 125             


Phe Tyr Ser Lys Phe Leu Asp Asp Leu His Ala Ala Gly Ile Glu Pro 
    130                 135                 140                 


Phe Val Thr Leu Phe His Trp Asp Leu Pro Asp Glu Leu Met Lys Arg 
145                 150                 155                 160 


Tyr Gly Gly Met Leu Asn Lys Glu Glu Phe Val Ala Asp Tyr Ala Asn 
                165                 170                 175     


Tyr Ala Arg Val Val Phe Asn Ala Leu Gly Ser Lys Val Lys His Trp 
            180                 185                 190         


Ile Thr Phe Asn Glu Pro Trp Cys Ser Ser Val Leu Gly His Asn Thr 
        195                 200                 205             


Gly Lys His Ala Pro Gly Arg Thr Ser Asp Arg Thr Lys Ser Pro Glu 
    210                 215                 220                 


Gly Asp Gly Thr Arg Glu Pro Trp Ile Val Gly His Asn Leu Leu Val 
225                 230                 235                 240 


Ala His Gly Thr Val Val Asp Ile Tyr Arg Arg Glu Phe Lys Glu Lys 
                245                 250                 255     


Gln Gly Gly Glu Ile Gly Ile Thr Leu Asn Gly Asp Trp Ala Glu Pro 
            260                 265                 270         


Trp Asp Pro Glu Asn Pro Ala Asp Val Glu Ala Cys Asp Arg Lys Ile 
        275                 280                 285             


Glu Phe Ala Ile Ser Trp Phe Ala Asp Pro Ile Tyr His Gly Lys Tyr 
    290                 295                 300                 


Pro Asp Ser Met Val Lys Gln Leu Gly Asp Arg Leu Pro Lys Phe Thr 
305                 310                 315                 320 


Pro Glu Glu Ile Ala Phe Val His Gly Ser Asn Asp Phe Tyr Gly Met 
                325                 330                 335     


Asn His Tyr Cys Glu Asn Tyr Ile Arg Asn Arg Thr Gly Glu Pro Asp 
            340                 345                 350         


Pro Glu Asp Ile Ala Gly Asn Leu Asp Ile Leu Met Glu Asp Lys Asn 
        355                 360                 365             


Gly Asn Pro Ile Gly Pro Glu Thr Gln Cys Glu Trp Leu Arg Pro Phe 
    370                 375                 380                 


Pro Leu Gly Phe Arg Lys Leu Leu Lys Trp Leu Ala Asp Arg Tyr Asn 
385                 390                 395                 400 


Asn Pro Lys Ile Tyr Val Thr Glu Asn Gly Thr Ser Val Lys Gly Glu 
                405                 410                 415     


Ser Asp Lys Pro Leu Glu Glu Val Leu Asn Asp Glu Phe Arg Val Gln 
            420                 425                 430         


Tyr Tyr Arg Asp Tyr Ile Gly Ala Met Val Asp Ala Val Ala Gln Asp 
        435                 440                 445             


Gly Val Asn Val Lys Ala Tyr Met Ala Trp Ser Leu Leu Asp Asn Phe 
    450                 455                 460                 


Glu Trp Ser Glu Gly Tyr Arg Ser Arg Phe Gly Val Thr Tyr Val Asp 
465                 470                 475                 480 


Tyr Lys Asn Gly Gln Lys Arg Ile Pro Lys Lys Ser Ala Leu Val Ile 
                485                 490                 495     


Gly Glu Leu Phe Asn Lys Tyr Ile Arg Lys Glu 
            500                 505         


<210>  25
<211>  1501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-glucosidase variant ZTB-4

<400>  25
atgtccttcc gctctcttct ggctctgtcc ggtctggtct gcaccggtct ggcttctgcc       60

actgcctcca ccctccctcc tgacttcctc tggggtttcg ccactgccag ctaccagatt      120

gagggtgctg ttgatgagga tggccgtgga ccttccatct gggatacctt ctgcgctatc      180

cccggcaaga ttgccgatgg ctcttctggc gatgttgctt gcgacagcta ccaccgtacc      240

cacgaagata ttgctctcct caagtcctac ggtgtcaacg cctaccgctt ctccatctcc      300

tggagccgta tcatccctct tggtggccgc aacgaccctg tcaacgagaa gggcattgac      360

cactactcca agttcgtcga tgatttgctt gctgctggta tcaccccctt cgtcaccctc      420

ttccactggg atcttcccca ggctctcgag gaccgctacg gtggtctgct caacaaggaa      480

gagttcgtcg ccgacttcga gaactacgcc cgtgtcatgt tcgagcgctt cggtgaccgt      540

gtcaagcact ggatcacctt caacgagccc tggtgctctt ccgtccttgg atacaacacc      600

ggccagttcg ctcctggtcg tacctccgac cgcagcaagt cccccgttgg tgactcttcc      660

accgagccct ggattgtcgg ccacaacctg cttgttgctc acggtgctgc cgtcaagatc      720

taccgtgagg agttcaagcc tacccagggt ggtgagatcg gtatcaccct caacggtgac      780

tgggctgagc cctgggaccc cgagaacccc gccgatgttg aagcctgcga ccgcaagatc      840

gagttcgcca tctcctggtt cgccgacccc atctaccacg gtgactaccc tgacagcatg      900

cgcaagcagc tcggtgaccg tctgcccacc ttcacccccg aagaagtcgc tctggtcaag      960

ggcagcaacg acttctacgg catgaaccac tacactgcca actacatccg tcaccgtacc     1020

ggtgagcctg accccaacga cactgctggc aacctggaga tcctgctcca gaacaagaac     1080

ggcaactcga ttggtcccga gactcagtcc ccctggctac gccctcacgc tcagggtttc     1140

cgcaagctcc tcaactggct ctccgaccgc tacaactacc ccaagatcta cgtgaccgag     1200

aacggcacct cgctgaaggg tgagaacgac ctccccttgg agcagcttct gaacgatgac     1260

ttccgtgtgc agtacttccg tgactacatc ggtgccatgg ccgatgccta caccctcgat     1320

ggtgtcaacg tccgtgccta catggcctgg tcgctgatgg acaacttcga atgggcggaa     1380

ggctacgaga ctcgcttcgg tgtgacctac gttgactacg agaacggcca gaagcgctac     1440

cccaagaagt ctgctctggt catcggtgat atcttcgaca gcttgattga gaaggcataa     1500

a                                                                     1501


<210>  26
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Beta-glucosidase variant ZTB-4

<400>  26

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      


Leu Ala Ser Ala Thr Ala Ser Thr Leu Pro Pro Asp Phe Leu Trp Gly 
            20                  25                  30          


Phe Ala Thr Ala Ser Tyr Gln Ile Glu Gly Ala Val Asp Glu Asp Gly 
        35                  40                  45              


Arg Gly Pro Ser Ile Trp Asp Thr Phe Cys Ala Ile Pro Gly Lys Ile 
    50                  55                  60                  


Ala Asp Gly Ser Ser Gly Asp Val Ala Cys Asp Ser Tyr His Arg Thr 
65                  70                  75                  80  


His Glu Asp Ile Ala Leu Leu Lys Ser Tyr Gly Val Asn Ala Tyr Arg 
                85                  90                  95      


Phe Ser Ile Ser Trp Ser Arg Ile Ile Pro Leu Gly Gly Arg Asn Asp 
            100                 105                 110         


Pro Val Asn Glu Lys Gly Ile Asp His Tyr Ser Lys Phe Val Asp Asp 
        115                 120                 125             


Leu Leu Ala Ala Gly Ile Thr Pro Phe Val Thr Leu Phe His Trp Asp 
    130                 135                 140                 


Leu Pro Gln Ala Leu Glu Asp Arg Tyr Gly Gly Leu Leu Asn Lys Glu 
145                 150                 155                 160 


Glu Phe Val Ala Asp Phe Glu Asn Tyr Ala Arg Val Met Phe Glu Arg 
                165                 170                 175     


Phe Gly Asp Arg Val Lys His Trp Ile Thr Phe Asn Glu Pro Trp Cys 
            180                 185                 190         


Ser Ser Val Leu Gly Tyr Asn Thr Gly Gln Phe Ala Pro Gly Arg Thr 
        195                 200                 205             


Ser Asp Arg Ser Lys Ser Pro Val Gly Asp Ser Ser Thr Glu Pro Trp 
    210                 215                 220                 


Ile Val Gly His Asn Leu Leu Val Ala His Gly Ala Ala Val Lys Ile 
225                 230                 235                 240 


Tyr Arg Glu Glu Phe Lys Pro Thr Gln Gly Gly Glu Ile Gly Ile Thr 
                245                 250                 255     


Leu Asn Gly Asp Trp Ala Glu Pro Trp Asp Pro Glu Asn Pro Ala Asp 
            260                 265                 270         


Val Glu Ala Cys Asp Arg Lys Ile Glu Phe Ala Ile Ser Trp Phe Ala 
        275                 280                 285             


Asp Pro Ile Tyr His Gly Asp Tyr Pro Asp Ser Met Arg Lys Gln Leu 
    290                 295                 300                 


Gly Asp Arg Leu Pro Thr Phe Thr Pro Glu Glu Val Ala Leu Val Lys 
305                 310                 315                 320 


Gly Ser Asn Asp Phe Tyr Gly Met Asn His Tyr Thr Ala Asn Tyr Ile 
                325                 330                 335     


Arg His Arg Thr Gly Glu Pro Asp Pro Asn Asp Thr Ala Gly Asn Leu 
            340                 345                 350         


Glu Ile Leu Leu Gln Asn Lys Asn Gly Asn Ser Ile Gly Pro Glu Thr 
        355                 360                 365             


Gln Ser Pro Trp Leu Arg Pro His Ala Gln Gly Phe Arg Lys Leu Leu 
    370                 375                 380                 


Asn Trp Leu Ser Asp Arg Tyr Asn Tyr Pro Lys Ile Tyr Val Thr Glu 
385                 390                 395                 400 


Asn Gly Thr Ser Leu Lys Gly Glu Asn Asp Leu Pro Leu Glu Gln Leu 
                405                 410                 415     


Leu Asn Asp Asp Phe Arg Val Gln Tyr Phe Arg Asp Tyr Ile Gly Ala 
            420                 425                 430         


Met Ala Asp Ala Tyr Thr Leu Asp Gly Val Asn Val Arg Ala Tyr Met 
        435                 440                 445             


Ala Trp Ser Leu Met Asp Asn Phe Glu Trp Ala Glu Gly Tyr Glu Thr 
    450                 455                 460                 


Arg Phe Gly Val Thr Tyr Val Asp Tyr Glu Asn Gly Gln Lys Arg Tyr 
465                 470                 475                 480 


Pro Lys Lys Ser Ala Leu Val Ile Gly Asp Ile Phe Asp Ser Leu Ile 
                485                 490                 495     


Glu Lys Ala 
            


<210>  27
<211>  715
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Endoglucanase wild-type ZTC-WT

<400>  27
atgttcaagg ctctccttgc tgttggtttc gccattgctc tgaccttcgc ctccgctgct       60

cagaccatca ccggccagta cgactgcatt cctgctggtg cctacaccct ctgccagaac      120

ctctggggtg agtacgctgg tgttggcagc cagaacagca ctctgatctc caccaacggc      180

aacgccgtca cctggcagac caactggacc tgggccaaca accccaacac tgtcaagtcc      240

tgcgcttctc actctttccc cattgccagc tctgctccta ctgcctggaa ctggacctac      300

gtgaccgaga gccagggtat ccgtgccgat gtctcctacg acatctggtt cggcaaggcc      360

cagtccggca accccgccac ctccgccagc agctacgaga tcatgatctg gctatccggt      420

cttggtggta tccagcccgt cggtcaccag atcctttccg gtctgaacat tgctggtcac      480

acctggaacc tctggtccgg tcccaactcc aactggcagg tcttctcctt cgtcatctcc      540

tctggcgagg tccgcaactt ctctgccgac ctcaacgagt tcttccagta cctgatccag      600

tcccagggtg ttgcctccac tcagtacctc caggccatcc aggtcggtac tgagcccttc      660

gtcggctctg cctccctcct gaccgagtcc ttcgctgttg ctgtcaatgt ttaaa           715


<210>  28
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endoglucanase wild-type ZTC-WT

<400>  28

Met Phe Lys Ala Leu Leu Ala Val Gly Phe Ala Ile Ala Leu Thr Phe 
1               5                   10                  15      


Ala Ser Ala Ala Gln Thr Ile Thr Gly Gln Tyr Asp Cys Ile Pro Ala 
            20                  25                  30          


Gly Ala Tyr Thr Leu Cys Gln Asn Leu Trp Gly Glu Tyr Ala Gly Val 
        35                  40                  45              


Gly Ser Gln Asn Ser Thr Leu Ile Ser Thr Asn Gly Asn Ala Val Thr 
    50                  55                  60                  


Trp Gln Thr Asn Trp Thr Trp Ala Asn Asn Pro Asn Thr Val Lys Ser 
65                  70                  75                  80  


Cys Ala Ser His Ser Phe Pro Ile Ala Ser Ser Ala Pro Thr Ala Trp 
                85                  90                  95      


Asn Trp Thr Tyr Val Thr Glu Ser Gln Gly Ile Arg Ala Asp Val Ser 
            100                 105                 110         


Tyr Asp Ile Trp Phe Gly Lys Ala Gln Ser Gly Asn Pro Ala Thr Ser 
        115                 120                 125             


Ala Ser Ser Tyr Glu Ile Met Ile Trp Leu Ser Gly Leu Gly Gly Ile 
    130                 135                 140                 


Gln Pro Val Gly His Gln Ile Leu Ser Gly Leu Asn Ile Ala Gly His 
145                 150                 155                 160 


Thr Trp Asn Leu Trp Ser Gly Pro Asn Ser Asn Trp Gln Val Phe Ser 
                165                 170                 175     


Phe Val Ile Ser Ser Gly Glu Val Arg Asn Phe Ser Ala Asp Leu Asn 
            180                 185                 190         


Glu Phe Phe Gln Tyr Leu Ile Gln Ser Gln Gly Val Ala Ser Thr Gln 
        195                 200                 205             


Tyr Leu Gln Ala Ile Gln Val Gly Thr Glu Pro Phe Val Gly Ser Ala 
    210                 215                 220                 


Ser Leu Leu Thr Glu Ser Phe Ala Val Ala Val Asn Val 
225                 230                 235         


<210>  29
<211>  736
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Endoglucanase variant ZTC-5

<400>  29
atgttcaagg ctctccttgc tgttggtttc gccattgctc tgaccttcgc ctccgctcag       60

accttgactg gccagtacga ctgcgctcct gctggtgcct acaccctctg ccagaacctc      120

tggggtgagg acaacggtgt tggcagccag aactcgaccc tcatctccac ttctggcaac      180

accatctcct ggtccaccac ctacacctgg gccaacaacc ccaacgatgt caagtcctac      240

gccaacgtcc tctccaacac tgccaagggc atgaccctct ctgccgtccg cgctgctcct      300

accacctggc agtgggaata cgagagcaag tcctccggtc tgcgcgccga tgtctcctac      360

gacatctggt tcggtactgc tccctccggt gaccccgcca cttctgcttc ttcctacgag      420

atcatgatct ggctatccca gcgtggtggt atccagcctg ttggcagcaa gatcctctcc      480

ggtatctccg ttgctggcca cacctggaac ctctacgagg gtcccaactc caactgggaa      540

acctactctt tcgtcagcgc cgatggtgac attcgtgact tccgtgccga tctgaaggac      600

ttcttcgact accttgttga gaacgagggt gtctccgaca gccagtacct gcaggccatt      660

cagactggta ccgagccctt caccggctct gccaccctca acgtcgagtc cttctccgtt      720

gccgtcaatg tctaaa                                                      736


<210>  30
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endoglucanase variant ZTC-5

<400>  30

Met Phe Lys Ala Leu Leu Ala Val Gly Phe Ala Ile Ala Leu Thr Phe 
1               5                   10                  15      


Ala Ser Ala Gln Thr Leu Thr Gly Gln Tyr Asp Cys Ala Pro Ala Gly 
            20                  25                  30          


Ala Tyr Thr Leu Cys Gln Asn Leu Trp Gly Glu Asp Asn Gly Val Gly 
        35                  40                  45              


Ser Gln Asn Ser Thr Leu Ile Ser Thr Ser Gly Asn Thr Ile Ser Trp 
    50                  55                  60                  


Ser Thr Thr Tyr Thr Trp Ala Asn Asn Pro Asn Asp Val Lys Ser Tyr 
65                  70                  75                  80  


Ala Asn Val Leu Ser Asn Thr Ala Lys Gly Met Thr Leu Ser Ala Val 
                85                  90                  95      


Arg Ala Ala Pro Thr Thr Trp Gln Trp Glu Tyr Glu Ser Lys Ser Ser 
            100                 105                 110         


Gly Leu Arg Ala Asp Val Ser Tyr Asp Ile Trp Phe Gly Thr Ala Pro 
        115                 120                 125             


Ser Gly Asp Pro Ala Thr Ser Ala Ser Ser Tyr Glu Ile Met Ile Trp 
    130                 135                 140                 


Leu Ser Gln Arg Gly Gly Ile Gln Pro Val Gly Ser Lys Ile Leu Ser 
145                 150                 155                 160 


Gly Ile Ser Val Ala Gly His Thr Trp Asn Leu Tyr Glu Gly Pro Asn 
                165                 170                 175     


Ser Asn Trp Glu Thr Tyr Ser Phe Val Ser Ala Asp Gly Asp Ile Arg 
            180                 185                 190         


Asp Phe Arg Ala Asp Leu Lys Asp Phe Phe Asp Tyr Leu Val Glu Asn 
        195                 200                 205             


Glu Gly Val Ser Asp Ser Gln Tyr Leu Gln Ala Ile Gln Thr Gly Thr 
    210                 215                 220                 


Glu Pro Phe Thr Gly Ser Ala Thr Leu Asn Val Glu Ser Phe Ser Val 
225                 230                 235                 240 


Ala Val Asn Val 
                


