
                                SEQUENCE LISTING

<110> Baker, Jeffrey P.
      Cheek, Robert L.
      Dixon, Eric P.
      Fischer, Timothy J.
      Knapp, Steven L.
      Simkins, Stephen G.
      Whitehead, Clark M.

<120> MATRIX METALLOPROTEINASE-7 (MMP-7)
  MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE
  DETECTION OF OVARIAN CANCER

<130> 046143/386567

<150> 61/157,650          
<151> 2009-03-05  

<160> 5

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 267
<212> PRT
<213> Homo sapiens

<400> 1
Met Arg Leu Thr Val Leu Cys Ala Val Cys Leu Leu Pro Gly Ser Leu
 1               5                  10                  15      
Ala Leu Pro Leu Pro Gln Glu Ala Gly Gly Met Ser Glu Leu Gln Trp
            20                  25                  30          
Glu Gln Ala Gln Asp Tyr Leu Lys Arg Phe Tyr Leu Tyr Asp Ser Glu
        35                  40                  45              
Thr Lys Asn Ala Asn Ser Leu Glu Ala Lys Leu Lys Glu Met Gln Lys
    50                  55                  60                  
Phe Phe Gly Leu Pro Ile Thr Gly Met Leu Asn Ser Arg Val Ile Glu
65                  70                  75                  80  
Ile Met Gln Lys Pro Arg Cys Gly Val Pro Asp Val Ala Glu Tyr Ser
                85                  90                  95      
Leu Phe Pro Asn Ser Pro Lys Trp Thr Ser Lys Val Val Thr Tyr Arg
            100                 105                 110         
Ile Val Ser Tyr Thr Arg Asp Leu Pro His Ile Thr Val Asp Arg Leu
        115                 120                 125             
Val Ser Lys Ala Leu Asn Met Trp Gly Lys Glu Ile Pro Leu His Phe
    130                 135                 140                 
Arg Lys Val Val Trp Gly Thr Ala Asp Ile Met Ile Gly Phe Ala Arg
145                 150                 155                 160 
Gly Ala His Gly Asp Ser Tyr Pro Phe Asp Gly Pro Gly Asn Thr Leu
                165                 170                 175     
Ala His Ala Phe Ala Pro Gly Thr Gly Leu Gly Gly Asp Ala His Phe
            180                 185                 190         
Asp Glu Asp Glu Arg Trp Thr Asp Gly Ser Ser Leu Gly Ile Asn Phe
        195                 200                 205             
Leu Tyr Ala Ala Thr His Glu Leu Gly His Ser Leu Gly Met Gly His
    210                 215                 220                 
Ser Ser Asp Pro Asn Ala Val Met Tyr Pro Thr Tyr Gly Asn Gly Asp
225                 230                 235                 240 
Pro Gln Asn Phe Lys Leu Ser Gln Asp Asp Ile Lys Gly Ile Gln Lys
                245                 250                 255     
Leu Tyr Gly Lys Arg Ser Asn Ser Arg Lys Lys
            260                 265         


<210> 2
<211> 1147
<212> DNA
<213> Homo sapiens

<400> 2
accaaatcaa ccataggtcc aagaacaatt gtctctggac ggcagctatg cgactcaccg 60
tgctgtgtgc tgtgtgcctg ctgcctggca gcctggccct gccgctgcct caggaggcgg 120
gaggcatgag tgagctacag tgggaacagg ctcaggacta tctcaagaga ttttatctct 180
atgactcaga aacaaaaaat gccaacagtt tagaagccaa actcaaggag atgcaaaaat 240
tctttggcct acctataact ggaatgttaa actcccgcgt catagaaata atgcagaagc 300
ccagatgtgg agtgccagat gttgcagaat actcactatt tccaaatagc ccaaaatgga 360
cttccaaagt ggtcacctac aggatcgtat catatactcg agacttaccg catattacag 420
tggatcgatt agtgtcaaag gctttaaaca tgtggggcaa agagatcccc ctgcatttca 480
ggaaagttgt atggggaact gctgacatca tgattggctt tgcgcgagga gctcatgggg 540
actcctaccc atttgatggg ccaggaaaca cgctggctca tgcctttgcg cctgggacag 600
gtctcggagg agatgctcac ttcgatgagg atgaacgctg gacggatggt agcagtctag 660
ggattaactt cctgtatgct gcaactcatg aacttggcca ttctttgggt atgggacatt 720
cctctgatcc taatgcagtg atgtatccaa cctatggaaa tggagatccc caaaatttta 780
aactttccca ggatgatatt aaaggcattc agaaactata tggaaagaga agtaattcaa 840
gaaagaaata gaaacttcag gcagaacatc cattcattca ttcattggat tgtatatcat 900
tgttgcacaa tcagaattga taagcactgt tcctccactc catttagcaa ttatgtcacc 960
cttttttatt gcagttggtt tttgaatgtc tttcactcct tttaaggata aactccttta 1020
tggtgtgact gtgtcttatt catctatact tgcagtgggt agatgtcaat aaatgttaca 1080
tacacaaata aataaaatgt ttattccatg gtaaatttaa aaaaaaaaaa aaaaaaaaaa 1140
aaaaaaa                                                           1147

<210> 3
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope sequence for MMP-7 monoclonal antibody
      designated as 5G11.9

<400> 3
Ser Tyr Thr Arg Asp Leu Pro His Ile Thr Val Asp Arg Leu Val Ser
 1               5                  10                  15      
Lys Ala Leu Asn
            20  


<210> 4
<211> 63
<212> PRT
<213> Artificial Sequence

<220> 
<223> Epitope sequence for MMP-7 monoclonal antibody
      designated as 15H8.12

<400> 4
Asp Ser Glu Thr Lys Asn Ala Asn Ser Leu Glu Ala Lys Leu Lys Glu
 1               5                  10                  15      
Met Gln Lys Phe Phe Gly Leu Pro Ile Thr Gly Met Leu Asn Ser Arg
            20                  25                  30          
Val Ile Glu Ile Met Gln Lys Pro Arg Cys Gly Val Pro Asp Val Ala
        35                  40                  45              
Glu Tyr Ser Leu Phe Pro Asn Ser Pro Lys Trp Thr Ser Lys Val
    50                  55                  60              


<210> 5
<211> 279
<212> PRT
<213> Artificial Sequence

<220> 
<223> MMP-7 sequence fused to a small polypeptide linker
      and a carboxy-terminal hexahistidine tag
      (immunogenic polypeptide)

<400> 5
Met Arg Leu Thr Val Leu Cys Ala Val Cys Leu Leu Pro Gly Ser Leu
 1               5                  10                  15      
Ala Leu Pro Leu Pro Gln Glu Ala Gly Gly Met Ser Glu Leu Gln Trp
            20                  25                  30          
Glu Gln Ala Gln Asp Tyr Leu Lys Arg Phe Tyr Leu Tyr Asp Ser Glu
        35                  40                  45              
Thr Lys Asn Ala Asn Ser Leu Glu Ala Lys Leu Lys Glu Met Gln Lys
    50                  55                  60                  
Phe Phe Gly Leu Pro Ile Thr Gly Met Leu Asn Ser Arg Val Ile Glu
65                  70                  75                  80  
Ile Met Gln Lys Pro Arg Cys Gly Val Pro Asp Val Ala Glu Tyr Ser
                85                  90                  95      
Leu Phe Pro Asn Ser Pro Lys Trp Thr Ser Lys Val Val Thr Tyr Arg
            100                 105                 110         
Ile Val Ser Tyr Thr Arg Asp Leu Pro His Ile Thr Val Asp Arg Leu
        115                 120                 125             
Val Ser Lys Ala Leu Asn Met Trp Gly Lys Glu Ile Pro Leu His Phe
    130                 135                 140                 
Arg Lys Val Val Trp Gly Thr Ala Asp Ile Met Ile Gly Phe Ala Arg
145                 150                 155                 160 
Gly Ala His Gly Asp Ser Tyr Pro Phe Asp Gly Pro Gly Asn Thr Leu
                165                 170                 175     
Ala His Ala Phe Ala Pro Gly Thr Gly Leu Gly Gly Asp Ala His Phe
            180                 185                 190         
Asp Glu Asp Glu Arg Trp Thr Asp Gly Ser Ser Leu Gly Ile Asn Phe
        195                 200                 205             
Leu Tyr Ala Ala Thr His Glu Leu Gly His Ser Leu Gly Met Gly His
    210                 215                 220                 
Ser Ser Asp Pro Asn Ala Val Met Tyr Pro Thr Tyr Gly Asn Gly Asp
225                 230                 235                 240 
Pro Gln Asn Phe Lys Leu Ser Gln Asp Asp Ile Lys Gly Ile Gln Lys
                245                 250                 255     
Leu Tyr Gly Lys Arg Ser Asn Ser Arg Lys Lys Glu Phe Leu Glu Gly
            260                 265                 270         
Gly His His His His His His
        275                 

