                         SEQUENCE LISTING

<110>  UWS Ventures Ltd.
       UWS Ventures Ltd.
 
<120>  Fructanase (Lactabacillus paracasei)

<130>  55004WO

<150>  GB0903227,7
<151>  2009-02-26

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  31
<212>  DNA
<213>  Primer

<400>  1
acgtagctag cgctacaagt gcttcgtcta c                                    31


<210>  2
<211>  51
<212>  DNA
<213>  Primer

<400>  2
cgtaggatcc tcagtggtgg tggtggtggt gtttttcagt tagttgacca g              51


<210>  3
<211>  712
<212>  PRT
<213>  Lactobacillus paracasei

<400>  3

Met Ala Ser Ala Ser Ser Ala Ser Ser Thr Gln Ile Ser Gln Thr Asn 
1               5                   10                  15      


Thr Gly Ser Gln Pro Asn Asn Glu Thr Thr Gly Glu Thr Ala Gln Ser 
            20                  25                  30          


Ser Val Asn Ser Thr Ala Thr Ala Ser Ser Ser Ser Val Ala Asp Leu 
        35                  40                  45              


Pro Ser Ser Ser Asp Ser Lys Ser Ser Ile Gly Ser Thr Ile Ser Gln 
    50                  55                  60                  


Pro Thr Val Asp Lys Lys Glu Thr Ser Lys Ser Asp Thr Ala Asp Asn 
65                  70                  75                  80  


Asp Leu Thr Lys Ser Val Thr Thr Ser Asp Ser Asp Lys Ala Leu Pro 
                85                  90                  95      


Thr Ser Lys Thr Thr Leu Pro Thr Ser Asn Glu Gln Val Gln Ser Ser 
            100                 105                 110         


Val Gly Gln Ser Gln Thr Asp Gln Pro Ala Ser Ser Ala Thr Ile Ala 
        115                 120                 125             


Thr Asn Ala Val Thr Ser Asp Val Ser Gln Asn Asp Gln Tyr Asn Glu 
    130                 135                 140                 


Pro Tyr Arg Asn Gln Tyr His Tyr Ser Ser Ser Gln Asn Trp Ile Asn 
145                 150                 155                 160 


Asp Pro Asn Gly Leu Phe Tyr Asp Ser Lys Thr Gly Leu Tyr Asn Leu 
                165                 170                 175     


Tyr Tyr Gln Tyr Asn Pro Glu Gly Asn Gln Trp Gly Asn Met Ser Trp 
            180                 185                 190         


Gly His Ala Val Ser Lys Asp Leu Ile Asn Trp Thr Gln Glu Asp Val 
        195                 200                 205             


Ala Ile Pro Met Leu Gln Asn Gln Gly Trp Glu Asp Phe Thr Tyr Thr 
    210                 215                 220                 


Asn Thr Thr Gly Ser Leu Lys Asp Lys Gly Glu Val Arg Tyr Val Gly 
225                 230                 235                 240 


Val Pro Thr Thr Asn Trp Gly Asp Ala Asp Gly Lys Lys Ala Ile Phe 
                245                 250                 255     


Ser Gly Ser Ile Val Val Asp Thr Asn Asn Val Ser Gly Leu Gly Lys 
            260                 265                 270         


Asp Ala Ile Leu Ala Phe Tyr Thr Ala Asp Tyr Gln Ile Ala Thr Arg 
        275                 280                 285             


Lys Asn Asp Gly Ala Glu Asp Gly Trp Gly Thr Trp Ile Gly Leu Thr 
    290                 295                 300                 


Glu Ile Gln Glu Gln His Leu Ala Tyr Ser Leu Asp Gly Gly Lys Thr 
305                 310                 315                 320 


Phe Ile Gln Tyr Ser Lys Asp Gly Asn Ala Ala Asn Pro Gln Ala Ile 
                325                 330                 335     


Ile Pro Thr Ser Met Asn Gln Gly Gly Asp Ala Ala Asn Phe Arg Asp 
            340                 345                 350         


Pro Ser Val Val Tyr Asp Ala Val Asn Lys Gln Tyr Tyr Leu Thr Val 
        355                 360                 365             


Val Ser Gly Gln Gln Ala Leu Ile Tyr Lys Ser Ser Asn Leu Leu Asp 
    370                 375                 380                 


Trp Thr Tyr Ala Ser Lys Ile Glu Arg Glu Asn Asp Val Gly Asn Gly 
385                 390                 395                 400 


Val Trp Glu Cys Pro Ser Leu Val Pro Met Lys Val Ala Gly Thr Asn 
                405                 410                 415     


Glu Thr Lys Trp Val Phe Cys Ile Ser Val Gln Gln Gly Ala His Ala 
            420                 425                 430         


Thr Gly Ser Gly Met Gln Tyr Tyr Val Gly Asn Met Thr Ala Asp Gly 
        435                 440                 445             


Thr Trp Val Pro Glu Ser Ser Lys Thr Leu Gln Asn Pro Met Thr Met 
    450                 455                 460                 


Asp Ser Gly Glu Asp Phe Tyr Ala Gly Ile Pro Phe Ser Asn Met Pro 
465                 470                 475                 480 


Asp Gly Arg Thr Val Met Leu Ala Trp Gln Ser Asn Trp Ser Tyr Val 
                485                 490                 495     


Asp Glu Ala Lys Thr Ser Pro Trp Ser Gly Asn Met Thr Leu Pro Arg 
            500                 505                 510         


Glu Leu Ser Leu Lys Lys Asn Ala Asp Thr Thr Asp Gly Tyr Leu Leu 
        515                 520                 525             


Thr Asn Thr Val Val Lys Glu Ile Ala Asn Asn Glu Glu Ala Asn Val 
    530                 535                 540                 


Ile Asn Lys Ala Glu Ser Asn Phe Thr Val Ser Arg Ser Asp Glu Gln 
545                 550                 555                 560 


Val Gln Tyr Glu Gly Lys Gln Tyr Lys Ile Ser Ala Thr Phe Ser Trp 
                565                 570                 575     


Asp Glu Ala Asp Lys Pro Lys Ser Val Gly Phe Lys Leu Arg Val Ser 
            580                 585                 590         


Asp Asp Gln Lys Tyr Asp Met Ile Val Gly Tyr Asp Leu Thr Thr Gly 
        595                 600                 605             


Leu Leu Tyr Val Gln Arg Leu Asn Thr Gly Glu Pro Asn Met Gly Ala 
    610                 615                 620                 


Pro Arg Asp Lys Met Asn Ala Thr Val Asn Ala Asp Gly Ser Ile Thr 
625                 630                 635                 640 


Ile Thr Val Tyr Val Asp Glu Thr Ser Ile Glu Ala Phe Ala Asn Asp 
                645                 650                 655     


Gly Glu Lys Ser Ile Thr Gln Asn Phe Phe Met Arg Pro Glu Asn Ile 
            660                 665                 670         


Gly Asp Gln Ala Thr Thr Gly Val Tyr Val Tyr Ser Asn Asp Gly Thr 
        675                 680                 685             


Thr Lys Ile Ser Asp Leu Thr Ile Asn Pro Ile Thr Ser Ile Trp Asn 
    690                 695                 700                 


Ser Thr Gly Gln Leu Thr Glu Lys 
705                 710         


<210>  4
<211>  1376
<212>  PRT
<213>  Lactobacillus paracasei

<400>  4

Met Glu Met Asp Glu Lys Lys His Tyr Lys Met Tyr Lys Ser Lys Ser 
1               5                   10                  15      


Val Trp Val Phe Ala Cys Leu Ser Thr Cys Leu Ile Val Ser Phe Phe 
            20                  25                  30          


Asn Asp Gly Gln Asn Val Ser Ala Ala Thr Ser Ala Ser Ser Thr Gln 
        35                  40                  45              


Ile Ser Gln Thr Asn Thr Gly Ser Gln Pro Asn Asn Glu Thr Thr Gly 
    50                  55                  60                  


Glu Thr Ala Gln Ser Ser Val Asn Ser Thr Ala Thr Ala Ser Ser Ser 
65                  70                  75                  80  


Ser Val Ala Asp Leu Pro Ser Ser Ser Asp Ser Lys Ser Ser Ile Gly 
                85                  90                  95      


Ser Thr Ile Ser Gln Pro Thr Val Asp Lys Lys Glu Thr Ser Lys Ser 
            100                 105                 110         


Asp Thr Ala Asp Asn Asp Leu Thr Lys Ser Val Thr Thr Ser Asp Ser 
        115                 120                 125             


Asp Lys Ala Leu Pro Thr Ser Lys Thr Thr Leu Pro Thr Ser Asn Glu 
    130                 135                 140                 


Gln Val Gln Ser Ser Val Gly Gln Ser Gln Thr Asp Gln Ser Ala Ser 
145                 150                 155                 160 


Ser Ala Thr Ile Ala Thr Asn Ala Val Thr Ser Asp Val Ser Gln Asn 
                165                 170                 175     


Asp Gln Tyr Asn Glu Pro Tyr Arg Asn Gln Tyr His Tyr Ser Ser Ser 
            180                 185                 190         


Gln Asn Trp Ile Asn Asp Pro Asn Gly Leu Phe Tyr Asp Ser Lys Thr 
        195                 200                 205             


Gly Leu Tyr Asn Leu Tyr Tyr Gln Tyr Asn Pro Glu Gly Asn Gln Trp 
    210                 215                 220                 


Gly Asn Met Ser Trp Gly His Ala Val Ser Lys Asp Leu Ile Asn Trp 
225                 230                 235                 240 


Thr Gln Glu Asp Val Ala Ile Pro Met Leu Gln Asn Gln Gly Trp Glu 
                245                 250                 255     


Asp Phe Thr Tyr Thr Asn Thr Thr Gly Ser Leu Lys Asp Lys Gly Glu 
            260                 265                 270         


Val Arg Tyr Val Gly Val Pro Thr Thr Asn Trp Gly Asp Ala Asp Gly 
        275                 280                 285             


Lys Lys Ala Ile Phe Ser Gly Ser Ile Val Val Asp Thr Asn Asn Val 
    290                 295                 300                 


Ser Gly Leu Gly Lys Asp Ala Ile Leu Ala Phe Tyr Thr Ala Asp Tyr 
305                 310                 315                 320 


Gln Ile Ala Thr Arg Lys Asn Asp Gly Ala Glu Asp Gly Trp Gly Thr 
                325                 330                 335     


Trp Ile Gly Leu Thr Glu Ile Gln Glu Gln His Leu Ala Tyr Ser Leu 
            340                 345                 350         


Asp Gly Gly Lys Thr Phe Ile Gln Tyr Ser Lys Asp Gly Asn Ala Ala 
        355                 360                 365             


Asn Pro Gln Ala Ile Ile Pro Thr Ser Met Asn Gln Gly Gly Asp Ala 
    370                 375                 380                 


Ala Asn Phe Arg Asp Pro Ser Val Val Tyr Asp Ala Val Asn Lys Gln 
385                 390                 395                 400 


Tyr Tyr Leu Thr Val Val Ser Gly Gln Gln Ala Leu Ile Tyr Lys Ser 
                405                 410                 415     


Ser Asn Leu Leu Asp Trp Thr Tyr Ala Ser Lys Ile Glu Arg Glu Asn 
            420                 425                 430         


Asp Val Gly Asn Gly Val Trp Glu Cys Pro Ser Leu Val Pro Met Lys 
        435                 440                 445             


Val Ala Gly Thr Asn Glu Thr Lys Trp Val Phe Cys Ile Ser Val Gln 
    450                 455                 460                 


Gln Gly Ala His Ala Thr Gly Ser Gly Met Gln Tyr Tyr Val Gly Asn 
465                 470                 475                 480 


Met Thr Ala Asp Gly Thr Trp Val Pro Glu Ser Ser Lys Thr Leu Gln 
                485                 490                 495     


Asn Pro Met Thr Met Asp Ser Gly Glu Asp Phe Tyr Ala Gly Ile Pro 
            500                 505                 510         


Phe Ser Asn Met Pro Asp Gly Arg Thr Val Met Leu Ala Trp Gln Ser 
        515                 520                 525             


Asn Trp Ser Tyr Val Asp Glu Ala Lys Thr Ser Pro Trp Ser Gly Asn 
    530                 535                 540                 


Met Thr Leu Pro Arg Glu Leu Ser Leu Lys Lys Asn Ala Asp Thr Thr 
545                 550                 555                 560 


Asp Gly Tyr Leu Leu Thr Asn Thr Val Val Lys Glu Ile Ala Asn Asn 
                565                 570                 575     


Glu Glu Ala Asn Val Ile Asn Lys Ala Glu Ser Asn Phe Thr Val Ser 
            580                 585                 590         


Arg Ser Asp Glu Gln Val Gln Tyr Glu Gly Lys Gln Tyr Lys Ile Ser 
        595                 600                 605             


Ala Thr Phe Ser Trp Asp Glu Ala Asp Lys Pro Lys Ser Val Gly Phe 
    610                 615                 620                 


Lys Leu Arg Val Ser Asp Asp Gln Lys Tyr Asp Met Ile Val Gly Tyr 
625                 630                 635                 640 


Asp Leu Thr Thr Gly Leu Leu Tyr Val Gln Arg Leu Asn Thr Gly Glu 
                645                 650                 655     


Pro Asn Met Gly Ala Pro Arg Asp Lys Met Asn Ala Thr Val Asn Ala 
            660                 665                 670         


Asp Gly Ser Ile Thr Ile Thr Val Tyr Val Asp Glu Thr Ser Ile Glu 
        675                 680                 685             


Ala Phe Ala Asn Asp Gly Glu Lys Ser Ile Thr Gln Asn Phe Phe Met 
    690                 695                 700                 


Arg Pro Glu Asn Ile Gly Asp Gln Ala Thr Thr Gly Val Tyr Val Tyr 
705                 710                 715                 720 


Ser Asn Asp Gly Thr Thr Lys Ile Ser Asp Leu Thr Ile Asn Pro Ile 
                725                 730                 735     


Thr Ser Ile Trp Asn Ser Thr Gly Gln Leu Thr Glu Lys Phe Val Asp 
            740                 745                 750         


Glu Asn Gly Asn Thr Ile Ala Ser Asp Lys Ile Gln Thr Gly Arg Val 
        755                 760                 765             


Gly Gln Ser Tyr Thr Ser Glu Ser Ala Thr Ile Pro Gly Tyr Val Phe 
    770                 775                 780                 


Val Lys Glu Asn Thr Asp His Ile Asn Ser Asn Gln Leu Tyr Thr Thr 
785                 790                 795                 800 


Gln Asn Gln Thr Ile Thr Tyr Thr Tyr Arg Ala Ser Gln Ala Ser Val 
                805                 810                 815     


Val Thr Lys Asp Thr Thr Leu Ala Ala Gly Pro Ser Ala Ala Trp Asn 
            820                 825                 830         


Ala Ala Asp Asn Leu Val Gly Ala Thr Asp Ala Asp Gly Asn Ala Leu 
        835                 840                 845             


Ala Val Ser Asp Leu Thr Val Asn Gly Ala Val Asp Pro Lys Thr Pro 
    850                 855                 860                 


Gly Thr Tyr Thr Val Thr Tyr Ser Tyr Thr Asp Ala Thr Gly Asn Lys 
865                 870                 875                 880 


Ile Ser Lys Lys Ala Thr Val Thr Val Ile Ala Ser Lys Ala Asp Ile 
                885                 890                 895     


Val Thr Lys Asp Thr Thr Met Val Ala Gly Ala Ser Thr Ile Trp Asn 
            900                 905                 910         


Ala Ala Asp Asn Phe Val Glu Ala Lys Asn Ala Asp Gly Asn Ala Leu 
        915                 920                 925             


Thr Val Ser Asp Leu Met Ile Asn Gly Thr Val Asp Ser Lys Thr Pro 
    930                 935                 940                 


Gly Thr Tyr Thr Val Thr Tyr Ser Tyr Thr Asp Ala Ala Gly Asn Lys 
945                 950                 955                 960 


Ile Asn Lys Glu Ala Ile Val Thr Val Ile Ala Ser Lys Ala Asp Ile 
                965                 970                 975     


Val Thr Lys Asp Thr Thr Met Val Ala Gly Pro Ser Ala Ala Trp Asn 
            980                 985                 990         


Ala Val Asp Asn Phe Val Glu Ala  Thr Gly Ala Asp Gly  Asn Ala Leu 
        995                 1000                 1005             


Ala Leu  Ser Asp Leu Thr Val  Asn Gly Ala Val Asp  Pro Lys Thr 
    1010                 1015                 1020             


Pro Gly  Thr Tyr Thr Val Thr  Tyr Ser Tyr Thr Asp  Pro Ala Gly 
    1025                 1030                 1035             


Asn Lys  Ile Ser Lys Glu Ala  Thr Val Thr Val Ile  Ala Ser Lys 
    1040                 1045                 1050             


Ala Asp  Ile Val Thr Lys Asp  Thr Thr Met Val Ala  Gly Pro Ser 
    1055                 1060                 1065             


Ala Thr  Trp Asn Ala Val Asp  Asn Phe Val Glu Ala  Thr Gly Ala 
    1070                 1075                 1080             


Asp Gly  Asn Ala Leu Ala Leu  Ser Asp Leu Thr Val  Asn Gly Ala 
    1085                 1090                 1095             


Val Asp  Pro Lys Thr Pro Gly  Thr Tyr Thr Val Thr  Tyr Ser Tyr 
    1100                 1105                 1110             


Thr Asp  Val Ala Gly Asn Lys  Ile Ser Lys Glu Ala  Ile Val Thr 
    1115                 1120                 1125             


Val Ile  Ala Ser Lys Ala Asp  Ile Val Thr Lys Asp  Thr Thr Lys 
    1130                 1135                 1140             


Val Ala  Gly Pro Ser Ala Thr  Trp Asn Ala Ala Asp  Asn Leu Val 
    1145                 1150                 1155             


Ile Ala  Thr Asp Ala Lys Gly  Asn Ala Leu Ala Leu  Ser Asn Leu 
    1160                 1165                 1170             


Thr Val  Thr Gly Ser Val Asp  Ser Lys Thr Pro Gly  Thr Tyr Thr 
    1175                 1180                 1185             


Val Thr  Tyr Ser Tyr Thr Asp  Ala Ala Gly Asn Lys  Ile Ser Lys 
    1190                 1195                 1200             


Glu Ala  Thr Val Thr Val Ile  Ala Ser Lys Ala Asp  Ile Val Thr 
    1205                 1210                 1215             


Lys Asp  Thr Thr Met Val Ala  Gly Pro Ser Ala Ala  Trp Asn Ala 
    1220                 1225                 1230             


Ala Asn  Asn Leu Val Ser Ala  Thr Asp Ala Asp Gly  Asn Ala Leu 
    1235                 1240                 1245             


Ala Met  Ser Asn Leu Thr Val  Thr Gly Thr Val Asp  Leu Lys Thr 
    1250                 1255                 1260             


Gln Gly  Thr Tyr Thr Val Thr  Tyr Thr Tyr Thr Asp  Val Ala Gly 
    1265                 1270                 1275             


Asn Lys  Ile Ser Lys Glu Ala  Thr Val Thr Val Leu  Thr Glu Lys 
    1280                 1285                 1290             


Glu Thr  Asn Ile Glu Asp Asn  Thr Gly Ser Ser Ile  Ser Asn Asp 
    1295                 1300                 1305             


Arg Glu  Asn Pro Pro Ala Ser  Ile Thr Gly Lys Gly  Gly Asp Asp 
    1310                 1315                 1320             


Ile His  Gln Asn Ala Lys Thr  Thr Met Thr Lys Lys  Lys Thr Glu 
    1325                 1330                 1335             


Thr Leu  Pro Gln Ala Gly Asn  His Val Asn Glu Leu  Ala Ile Val 
    1340                 1345                 1350             


Leu Gly  Gln Met Ile Leu Ala  Ile Cys Val Gly Gly  Ile Leu Trp 
    1355                 1360                 1365             


Leu Lys  Arg Arg Val Lys Arg  Val 
    1370                 1375     


