                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
 
<120>  Conversion of unipotent germline stem cells into pluripotent stem
       cells without exogenous factors

<130>  P1977 PCT

<150>  EP 09 00 2141.1
<151>  2009-02-16 

<160>  62    

<170>  PatentIn version 3.3

<210>  1
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4 forward primer"

<400>  1
ctgagggcca ggcaggagca cgag                                            24


<210>  2
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4 reverse primer"

<400>  2
ctgtagggag ggcttcgggc actt                                            24


<210>  3
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Sox2 forward primer"

<400>  3
gggtgcaaaa agaggagagt ag                                              22


<210>  4
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Sox2 reverse primer"

<400>  4
gcgcctaacg taccactaga ac                                              22


<210>  5
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: c-Myc forward primer"

<400>  5
cagaggagga acgagctgaa gcgc                                            24


<210>  6
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: c-Myc reverse primer"

<400>  6
ttatgcacca gagtttcgaa gctgttc                                         27


<210>  7
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog forward primer"

<400>  7
agggtctgct actgagatgc tctg                                            24


<210>  8
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog reverse primer"

<400>  8
caaccactgg tttttctgcc accg                                            24


<210>  9
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Dazl forward primer"

<400>  9
gcactcagtc ttcatcagca ac                                              22


<210>  10
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Dazl reverse primer"

<400>  10
ctatcttctg cacatccacg tc                                              22


<210>  11
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Stra8 forward primer"

<400>  11
ccagtctgat atcacagcct ca                                              22


<210>  12
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Stra8 reverse primer"

<400>  12
ttccttgacc tcctctaagc tg                                              22


<210>  13
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Tex18 forward primer"

<400>  13
ggggagggag tagtacctgt tt                                              22


<210>  14
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Tex18 reverse primer"

<400>  14
ccacaccctg gatacttcac tt                                              22


<210>  15
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Fig-a forward primer"

<400>  15
ggagcgaagg tctcagagaa ac                                              22


<210>  16
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Fig-a reverse primer"

<400>  16
tggtagctgt acaatggctc ac                                              22


<210>  17
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Piwil2 forward primer"

<400>  17
cctcctgtaa ctgggaactt gg                                              22


<210>  18
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Piwil2 reverse primer"

<400>  18
gcaccacaac accctactat ga                                              22


<210>  19
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Rex1 forward primer"

<400>  19
caccatccgg gatgaaagtg agat                                            24


<210>  20
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Rex1 reverse primer"

<400>  20
accagaaaat gtcgctttag tttc                                            24


<210>  21
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Utf1 forward primer"

<400>  21
ctcaaggaca aactccgaga ct                                              22


<210>  22
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Utf1 reverse primer"

<400>  22
agacttcgtc gtggaagaac tg                                              22


<210>  23
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: c-kit forward primer"

<400>  23
cagtgtgtta cgtggtcatg tg                                              22


<210>  24
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: c-kit reverse primer"

<400>  24
gtgtagcact caagtcgaaa cg                                              22


<210>  25
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: VASA forward primer"

<400>  25
cttgcagaga tgttcagcag ac                                              22


<210>  26
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: VASA reverse primer"

<400>  26
ctccaagagc ttgctctctc tc                                              22


<210>  27
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Eras forward primer"

<400>  27
tctgcgtgac cagtgcttgg c                                               21


<210>  28
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Eras reverse primer"

<400>  28
tcttcaggct acagagcagc c                                               21


<210>  29
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Cripto forward primer"

<400>  29
atggacgcaa ctgtgaacat gatgttcgca                                      30


<210>  30
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Cripto reverse primer"

<400>  30
ctttgaggtc ctggtccatc acgtgaccat                                      30


<210>  31
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Esg1 forward primer"

<400>  31
ataagcttga tctcgtcttc c                                               21


<210>  32
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Esg1 reverse primer"

<400>  32
cttgctagga tgtaacaaag c                                               21


<210>  33
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: FGF4 forward primer"

<400>  33
cagcgaggcg tggtgagcat cttcgga                                         27


<210>  34
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: FGF4 reverse primer"

<400>  34
cttcttggtc cgcccgttct tactgag                                         27


<210>  35
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Zfp57 forward primer"

<400>  35
atcacttgtg ctgccaaaga c                                               21


<210>  36
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Zfp57 reverse primer"

<400>  36
cttctcctcc tggattccat c                                               21


<210>  37
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: PUM1 forward primer"

<400>  37
tggaggcggc tataatacta                                                 20


<210>  38
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: PUM1 reverse primer"

<400>  38
ttcttttcac ctttgtcgtt                                                 20


<210>  39
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: PUM2 forward primer"

<400>  39
tagaatctcg gggaatggga                                                 20


<210>  40
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: PUM2 reverse primer"

<400>  40
tggtttattc gctgtcggat                                                 20


<210>  41
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: GFR-a forward primer"

<400>  41
actcctggat ttgctgatgt cgg                                             23


<210>  42
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: GFR-a reverse primer"

<400>  42
cgctgcggca ctcatcctt                                                  19


<210>  43
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Zfp145 forward primer"

<400>  43
tgctgcccaa ccacaagg                                                   18


<210>  44
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Zfp145 reverse primer"

<400>  44
cgctgaatga gccagtaaat                                                 20


<210>  45
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: b-Actin forward 
       primer"

<400>  45
cgtgcgtgac atcaaagaga agc                                             23


<210>  46
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: b-Actin reverse 
       primer"

<400>  46
atctgctgga aggtggacag tgag                                            24


<210>  47
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4, 1st. forward 
       primer"

<400>  47
gggattttta gattgggttt agaaaa                                          26


<210>  48
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4, 1st. reverse 
       primer"

<400>  48
ccaccctcta accttaacct ctaac                                           25


<210>  49
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4, 2nd. forward 
       primer"

<400>  49
tgaggagtgg ttttagaaat aattg                                           25


<210>  50
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oct3/4, 2nd. reverse 
       primer"

<400>  50
aatcctctca cccctacctt aaat                                            24


<210>  51
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog, 1st. forward 
       primer"

<400>  51
tttgtaggtg ggattaattg tgaa                                            24


<210>  52
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog, 1st. reverse 
       primer"

<400>  52
aaattttaaa caacaaccaa aaa                                             23


<210>  53
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog, 2nd. forward 
       primer"

<400>  53
tttgtaggtg ggattaattg tgaa                                            24


<210>  54
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Nanog, 2nd. reverse 
       primer"

<400>  54
aaaaaaacaa aacaccaacc aaat                                            24


<210>  55
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Igf2r, 1st. forward 
       primer"

<400>  55
gtagagtttt ttgaattttt ttgtt                                           25


<210>  56
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Igf2r, 1st. reverse 
       primer"

<400>  56
taaactataa ttctaattat accaaattac                                      30


<210>  57
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Igf2r, 2nd. forward 
       primer"

<400>  57
tggtattttt atgtatagtt aggatag                                         27


<210>  58
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: Igf2r, 2nd. reverse 
       primer"

<400>  58
aaaaattcta taatcaaaac caac                                            24


<210>  59
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: H19, 1st. forward 
       primer"

<400>  59
taaggagatt atgttttatt tttgga                                          26


<210>  60
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: H19, 1st. reverse 
       primer"

<400>  60
ccccctaata acatttataa cccc                                            24


<210>  61
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: H19, 2nd. forward 
       primer"

<400>  61
aaggagatta tgttttattt ttgga                                           25


<210>  62
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  /note="Description of artificial sequence: H19, 2nd. reverse 
       primer"

<400>  62
aaacttaaat aacccacaac attacc                                          26


