                         SEQUENCE LISTING

<110>  California Institute of Technology
       Arnold, Frances H.
       Heinselman, Pete
       Minshull, Jeremy
       Govindarajan, Sridhar
       Villalobos, Alan
 
<120>  STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASES

<130>  00016-046WO1

<140>  Not yet known
<141>  2010-03-12

<150>  US 61/205,284
<151>  2009-01-16

<150>  US 61/167,003
<151>  2009-04-06

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1083
<212>  DNA
<213>  Humicola insolens


<220>
<221>  CDS
<222>  (1)..(1083)

<400>  1
ggt aac ccc ttt gaa ggt gtt cag ctg tgg gct aat aac tat tat aga         48
Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg           
1               5                   10                  15                

tct gag gta cat aca ctg gcc att ccg caa att aca gac ccc gcg ttg         96
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu           
            20                  25                  30                    

cgt gcc gca gct agt gct gtg gct gag gtg cca agt ttt caa tgg ctg        144
Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu           
        35                  40                  45                        

gac aga aat gta aca gtg gat act ttg ttg gta cag act ttg tca gaa        192
Asp Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu Ser Glu           
    50                  55                  60                            

atc cgt gag gcc aat caa gca ggt gct aat ccc caa tat gca gcg caa        240
Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Gln Tyr Ala Ala Gln           
65                  70                  75                  80            

atc gtg gtc tat gat ctg ccc gat aga gac tgt gca gct gcc gcc tcg        288
Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser           
                85                  90                  95                

aat ggt gaa tgg gca ata gcg aac aac ggt gta aac aat tac aaa gct        336
Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala           
            100                 105                 110                   

tac att aat aga att aga gag ata ttg ata agt ttt tcg gac gtt aga        384
Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg           
        115                 120                 125                       

acg ata tta gtc att gag cca gat agt cta gct aat atg gtc aca aat        432
Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn           
    130                 135                 140                           

atg aat gtc ccg aag tgt tcc ggt gca gcc agc act tat agg gaa tta        480
Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu           
145                 150                 155                 160           

acc ata tat gca ctg aag caa ttg gat ctg cct cat gtc gct atg tac        528
Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr           
                165                 170                 175               

atg gat gcc ggc cac gct gga tgg tta ggc tgg ccg gca aac att cag        576
Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln           
            180                 185                 190                   

cca gcc gca gaa ttg ttt gcc aaa att tac gaa gat gct gga aag cct        624
Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro           
        195                 200                 205                       

aga gca gtg aga ggt ctt gca act aat gtt gct aat tac aat gca tgg        672
Arg Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp           
    210                 215                 220                           

tca gtt tca tcc cct cca cca tac aca agt cca aat cca aac tac gat        720
Ser Val Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp           
225                 230                 235                 240           

gaa aag cat tat atc gaa gca ttc aga ccc tta tta gaa gcc cgt ggt        768
Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly           
                245                 250                 255               

ttc cca gcc caa ttt ata gtg gat cag gga aga tca ggt aag caa cca        816
Phe Pro Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro           
            260                 265                 270                   

act ggc caa aag gag tgg ggg cat tgg tgt aat gct att ggc aca gga        864
Thr Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly           
        275                 280                 285                       

ttt ggt atg aga cct act gct aat acc ggt cac cag tat gtg gat gct        912
Phe Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala           
    290                 295                 300                           

ttt gtt tgg gtt aaa ccg ggc ggt gaa tgc gac ggg acc agc gat act        960
Phe Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr           
305                 310                 315                 320           

acg gcg gcc aga tat gat tat cat tgt ggt ctg gaa gat gca tta aaa       1008
Thr Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys           
                325                 330                 335               

cca gct cct gaa gcc ggc cag tgg ttc aac gaa tac ttc att caa ttg       1056
Pro Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu           
            340                 345                 350                   

ctt agg aac gct aac ccg ccc ttt taa                                   1083
Leu Arg Asn Ala Asn Pro Pro Phe                                           
        355                 360                                           


<210>  2
<211>  360
<212>  PRT
<213>  Humicola insolens

<400>  2

Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 
1               5                   10                  15      


Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 
            20                  25                  30          


Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 
        35                  40                  45              


Asp Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu Ser Glu 
    50                  55                  60                  


Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Gln Tyr Ala Ala Gln 
65                  70                  75                  80  


Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 
                85                  90                  95      


Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala 
            100                 105                 110         


Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 
        115                 120                 125             


Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 
    130                 135                 140                 


Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu 
145                 150                 155                 160 


Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr 
                165                 170                 175     


Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln 
            180                 185                 190         


Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro 
        195                 200                 205             


Arg Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 
    210                 215                 220                 


Ser Val Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 
225                 230                 235                 240 


Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly 
                245                 250                 255     


Phe Pro Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro 
            260                 265                 270         


Thr Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly 
        275                 280                 285             


Phe Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala 
    290                 295                 300                 


Phe Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr 
305                 310                 315                 320 


Thr Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys 
                325                 330                 335     


Pro Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu 
            340                 345                 350         


Leu Arg Asn Ala Asn Pro Pro Phe 
        355                 360 


<210>  3
<211>  1077
<212>  DNA
<213>  Hypocrea jecorina


<220>
<221>  CDS
<222>  (1)..(1077)

<400>  3
ggt aat cca ttc gtt ggg gtg aca ccc tgg gcg aac gcc tat tat gct         48
Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr Ala           
1               5                   10                  15                

tct gag gtt tca tcc cta gct att ccc tct tta aca ggt gca atg gct         96
Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala           
            20                  25                  30                    

aca gcc gcc gct gcc gtt gca aag gtc cct tcc ttc atg tgg ctg gat        144
Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Met Trp Leu Asp           
        35                  40                  45                        

act ttg gac aaa acc ccc tta atg gaa caa acg ttg gct gat ata cgt        192
Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile Arg           
    50                  55                  60                            

act gcg aat aaa aac ggc ggc aat tat gct gga caa ttt gtg gtt tat        240
Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr           
65                  70                  75                  80            

gac ctg ccg gat aga gat tgt gct gca cta gcg agc aac ggg gag tac        288
Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu Tyr           
                85                  90                  95                

agc att gcg gat ggc ggt gtc gca aag tac aaa aac tat ata gat act        336
Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp Thr           
            100                 105                 110                   

atc agg caa ata gtt gtc gaa tac agt gat att cgt acg ctg ctt gta        384
Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile Arg Thr Leu Leu Val           
        115                 120                 125                       

atc gaa ccc gat tcc tta gcg aac ttg gta aca aat cta ggt act ccg        432
Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu Gly Thr Pro           
    130                 135                 140                           

aag tgt gcg aac gcg cag agt gct tat ctt gag tgc atc aat tat gca        480
Lys Cys Ala Asn Ala Gln Ser Ala Tyr Leu Glu Cys Ile Asn Tyr Ala           
145                 150                 155                 160           

gtc acc cag ttg aat ttg cca aac gtt gca atg tat ctt gat gct ggt        528
Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala Gly           
                165                 170                 175               

cat gcc ggg tgg ttg ggt tgg cca gca aat cag gat ccc gct gcg cag        576
His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala Gln           
            180                 185                 190                   

ctg ttt gca aat gtt tac aaa aat gcc tca agt cct aga gcg ctg agg        624
Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg           
        195                 200                 205                       

ggt ctt gca aca aat gtt gct aat tac aac gga tgg aat att acc tca        672
Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr Ser           
    210                 215                 220                           

ccc cca tca tac aca caa gga aat gct gtt tac aat gaa aaa ctt tat        720
Pro Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu Lys Leu Tyr           
225                 230                 235                 240           

att cat gcc att ggt cca ctg ctg gct aat cac gga tgg agt aat gcc        768
Ile His Ala Ile Gly Pro Leu Leu Ala Asn His Gly Trp Ser Asn Ala           
                245                 250                 255               

ttt ttc att aca gat caa ggg aga agt ggt aaa caa cct act gga caa        816
Phe Phe Ile Thr Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly Gln           
            260                 265                 270                   

caa caa tgg ggt gac tgg tgt aat gtt atc ggt act ggg ttt ggc atc        864
Gln Gln Trp Gly Asp Trp Cys Asn Val Ile Gly Thr Gly Phe Gly Ile           
        275                 280                 285                       

aga cca tca gcg aat acg ggt gat tca ttg ttg gac tca ttt gtt tgg        912
Arg Pro Ser Ala Asn Thr Gly Asp Ser Leu Leu Asp Ser Phe Val Trp           
    290                 295                 300                           

gtt aaa ccc ggg ggt gaa tgt gat gga acg agt gat tct tct gct cca        960
Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Ser Ser Ala Pro           
305                 310                 315                 320           

agg ttc gat tct cat tgc gca tta cca gat gct ttg cag cca gca cct       1008
Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala Pro           
                325                 330                 335               

caa gca gga gct tgg ttc caa gct tat ttt gta caa tta ctg act aac       1056
Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu Thr Asn           
            340                 345                 350                   

gcc aat cct agt ttt cta taa                                           1077
Ala Asn Pro Ser Phe Leu                                                   
        355                                                               


<210>  4
<211>  358
<212>  PRT
<213>  Hypocrea jecorina

<400>  4

Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 
1               5                   10                  15      


Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala 
            20                  25                  30          


Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Met Trp Leu Asp 
        35                  40                  45              


Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile Arg 
    50                  55                  60                  


Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr 
65                  70                  75                  80  


Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu Tyr 
                85                  90                  95      


Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp Thr 
            100                 105                 110         


Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile Arg Thr Leu Leu Val 
        115                 120                 125             


Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu Gly Thr Pro 
    130                 135                 140                 


Lys Cys Ala Asn Ala Gln Ser Ala Tyr Leu Glu Cys Ile Asn Tyr Ala 
145                 150                 155                 160 


Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala Gly 
                165                 170                 175     


His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala Gln 
            180                 185                 190         


Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg 
        195                 200                 205             


Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr Ser 
    210                 215                 220                 


Pro Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu Lys Leu Tyr 
225                 230                 235                 240 


Ile His Ala Ile Gly Pro Leu Leu Ala Asn His Gly Trp Ser Asn Ala 
                245                 250                 255     


Phe Phe Ile Thr Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly Gln 
            260                 265                 270         


Gln Gln Trp Gly Asp Trp Cys Asn Val Ile Gly Thr Gly Phe Gly Ile 
        275                 280                 285             


Arg Pro Ser Ala Asn Thr Gly Asp Ser Leu Leu Asp Ser Phe Val Trp 
    290                 295                 300                 


Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Ser Ser Ala Pro 
305                 310                 315                 320 


Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala Pro 
                325                 330                 335     


Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu Thr Asn 
            340                 345                 350         


Ala Asn Pro Ser Phe Leu 
        355             


<210>  5
<211>  1077
<212>  DNA
<213>  Chaetomium thermophilium


<220>
<221>  CDS
<222>  (1)..(1077)

<400>  5
ggt aac cct ttc agt ggt gtg cag tta tgg gct aat act tac tat tct         48
Gly Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser           
1               5                   10                  15                

tca gaa gtc cac acc tta gct atc cca agc tta agt cca gaa tta gcg         96
Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu Ala           
            20                  25                  30                    

gct aag gcg gcg aaa gta gct gaa gtg cca tca ttc caa tgg tta gat        144
Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln Trp Leu Asp           
        35                  40                  45                        

aga aac gtg act gtg gat act ctg ttt tct ggt aca ctt gct gag ata        192
Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu Ile           
    50                  55                  60                            

agg gcg gct aac caa agg gga gct aat cca cca tat gct ggc atc ttt        240
Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe           
65                  70                  75                  80            

gtg gtt tat gac ctt cct gat aga gat tgt gct gcc gct gca agc aat        288
Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn           
                85                  90                  95                

ggt gaa tgg agt ata gct aac aac ggt gct aac aac tat aag aga tat        336
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr           
            100                 105                 110                   

atc gat aga att aga gaa ttg ttg att cag tac tca gat atc agg aca        384
Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr           
        115                 120                 125                       

att ttg gtt att gaa cca gac agt cta gca aat atg gtt act aac atg        432
Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met           
    130                 135                 140                           

aac gta caa aaa tgt tct aac gca gca tct acg tat aaa gaa ctg act        480
Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr           
145                 150                 155                 160           

gtg tat gca ttg aaa cag ttg aac ttg cca cac gta gcc atg tat atg        528
Val Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met           
                165                 170                 175               

gat gca ggt cac gcc ggc tgg tta ggc tgg ccc gct aat ata cag cct        576
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro           
            180                 185                 190                   

gcc gca gaa tta ttc gcg caa ata tac aga gac gct gga cgt ccg gct        624
Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro Ala           
        195                 200                 205                       

gcg gtc agg ggt ctt gcc act aac gtt gca aat tac aac gct tgg tca        672
Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser           
    210                 215                 220                           

ata gcg agt cct cca tcg tac aca agc cct aac cca aac tac gat gag        720
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu           
225                 230                 235                 240           

aag cat tac ata gaa gca ttt gct cct ttg ctt cgt aac caa ggt ttt        768
Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe           
                245                 250                 255               

gat gca aag ttt atc gtc gat acc gga aga aac ggc aag cag ccg aca        816
Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr           
            260                 265                 270                   

ggg cag cta gaa tgg ggg cac tgg tgc aat gtc aag ggt acg ggt ttc        864
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe           
        275                 280                 285                       

ggt gtt aga ccc acg gct aac act ggg cat gag ttg gtt gat gca ttc        912
Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe           
    290                 295                 300                           

gtt tgg gta aaa ccc gga gga gag tca gac ggt act tct gat act agt        960
Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Ser           
305                 310                 315                 320           

gct gcc aga tac gat tac cac tgt ggc ctt tct gat gct ttg aca cca       1008
Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Ser Asp Ala Leu Thr Pro           
                325                 330                 335               

gcc cct gaa gcc ggg caa tgg ttc cag gcc tac ttc gaa caa cta ttg       1056
Ala Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu           
            340                 345                 350                   

att aac gca aac cca cca tag                                           1077
Ile Asn Ala Asn Pro Pro                                                   
        355                                                               


<210>  6
<211>  358
<212>  PRT
<213>  Chaetomium thermophilium

<400>  6

Gly Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 
1               5                   10                  15      


Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu Ala 
            20                  25                  30          


Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln Trp Leu Asp 
        35                  40                  45              


Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu Ile 
    50                  55                  60                  


Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe 
65                  70                  75                  80  


Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn 
                85                  90                  95      


Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 
            100                 105                 110         


Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 
        115                 120                 125             


Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 
    130                 135                 140                 


Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr 
145                 150                 155                 160 


Val Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met 
                165                 170                 175     


Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro 
            180                 185                 190         


Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro Ala 
        195                 200                 205             


Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser 
    210                 215                 220                 


Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 
225                 230                 235                 240 


Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 
                245                 250                 255     


Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 
            260                 265                 270         


Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 
        275                 280                 285             


Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 
    290                 295                 300                 


Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Ser 
305                 310                 315                 320 


Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Ser Asp Ala Leu Thr Pro 
                325                 330                 335     


Ala Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu 
            340                 345                 350         


Ile Asn Ala Asn Pro Pro 
        355             


<210>  7
<211>  267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBD Linker


<220>
<221>  CDS
<222>  (1)..(267)

<400>  7
gct agc tgc tca agc gtc tgg ggc caa tgt ggt ggc cag aat tgg tcg         48
Ala Ser Cys Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser           
1               5                   10                  15                

ggt ccg act tgc tgt gct tcc gga agc aca tgc gtc tac tcc aac gac         96
Gly Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp           
            20                  25                  30                    

tat tac tcc cag tgt ctt ccc ggc gct gca agc tca agc tcg tcc acg        144
Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr           
        35                  40                  45                        

cgc gcc gcg tcg acg act tct cga gta tcc ccc aca aca tcc cgg tcg        192
Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser           
    50                  55                  60                            

agc tcc gcg acg cct cca cct ggt tct act act acc aga gta cct cca        240
Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro           
65                  70                  75                  80            

gtc gga tcg gga acc gct acg tat tca                                    267
Val Gly Ser Gly Thr Ala Thr Tyr Ser                                       
                85                                                        


<210>  8
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Ala Ser Cys Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 
1               5                   10                  15      


Gly Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 
            20                  25                  30          


Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 
        35                  40                  45              


Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 
    50                  55                  60                  


Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro 
65                  70                  75                  80  


Val Gly Ser Gly Thr Ala Thr Tyr Ser 
                85                  


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer CBH2L

<400>  9
gctgaacgtg tcatcggtta c                                                 21


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer RSQ3080

<400>  10
gcaacacctg gcaattcctt acc                                               23


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer CBH2LPCR

<400>  11
gctgaacgtg tcatcgttac ttag                                              24


