                         SEQUENCE LISTING

<110>  Liu, Jim
       Li, Ming
       Lan, Tang
       Ye, Liu
       Zheng, Liu
 
<120>  POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  11571.204-WO

<150>  US 61/150,528
<151>  2009-02-06

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  2340
<212>  DNA
<213>  Aspergillus terreus


<220>
<221>  CDS
<222>  (24)..(188)

<220>
<221>  sig_peptide
<222>  (24)..(83)

<220>
<221>  mat_peptide
<222>  (84)..(2226)

<220>
<221>  CDS
<222>  (238)..(276)

<220>
<221>  CDS
<222>  (326)..(441)

<220>
<221>  CDS
<222>  (484)..(592)

<220>
<221>  CDS
<222>  (644)..(872)

<220>
<221>  CDS
<222>  (923)..(1085)

<220>
<221>  CDS
<222>  (1135)..(1281)

<220>
<221>  CDS
<222>  (1329)..(1569)

<220>
<221>  CDS
<222>  (1615)..(2226)

<400>  1
ctacaagcac aaaagttttc agg atg aag tgg acc tcc tcg ctc ctc ctc tta       53
                          Met Lys Trp Thr Ser Ser Leu Leu Leu Leu         
                          -20                 -15                         

ctg tcc gtg atc ggt cag gct act cat gcc ctg acc cca gca gaa tgg        101
Leu Ser Val Ile Gly Gln Ala Thr His Ala Leu Thr Pro Ala Glu Trp           
-10                 -5              -1  1               5                 

cgc agc cag tca atc tac ttc ctg ttg acc gat cgc ttt ggt cga acg        149
Arg Ser Gln Ser Ile Tyr Phe Leu Leu Thr Asp Arg Phe Gly Arg Thr           
            10                  15                  20                    

gac aat tcc aca act gcc gct tgc gat acc tct gac aga gtgtgtacca         198
Asp Asn Ser Thr Thr Ala Ala Cys Asp Thr Ser Asp Arg                       
        25                  30                  35                        

ttcgatattg gacttggttg catgtactga tgtgtgcag gta tac tgc ggt ggt         252
                                           Val Tyr Cys Gly Gly            
                                                           40             

agc tgg cag gga atc atc aac caa gtatgaggga ggtgtcgcgg acagacccag       306
Ser Trp Gln Gly Ile Ile Asn Gln                                           
                45                                                        

ccgatactaa cgctgccag ctc gat tac atc caa ggg atg gga ttc act gcc       358
                     Leu Asp Tyr Ile Gln Gly Met Gly Phe Thr Ala          
                         50                  55                           

atc tgg atc acc ccg gtc act gga cag ttc tac gaa aac acg ggc gac        406
Ile Trp Ile Thr Pro Val Thr Gly Gln Phe Tyr Glu Asn Thr Gly Asp           
60                  65                  70                  75            

ggc acc tct tac cat gga tac tgg cag cag gac at  gtatatcctc             451
Gly Thr Ser Tyr His Gly Tyr Trp Gln Gln Asp Ile                           
                80                  85                                    

cctctccttc gagttccatg ctaatcatgc ag c tac gat ctc aac tac aac tac      505
                                      Tyr Asp Leu Asn Tyr Asn Tyr         
                                              90                          

gga acg gcc caa gac ctc aaa aac cta gcc aat gct ttg cac gag cgc        553
Gly Thr Ala Gln Asp Leu Lys Asn Leu Ala Asn Ala Leu His Glu Arg           
95                  100                 105                 110           

ggc atg tat ttg atg gtt gat gtg gtt gcc aac cac atg gtcagtcttg         602
Gly Met Tyr Leu Met Val Asp Val Val Ala Asn His Met                       
                115                 120                                   

ttcttttgga tatatgatag agacgtattg aaccttttta g ggc tat gac gga gcg      658
                                              Gly Tyr Asp Gly Ala         
                                                  125                     

gga aac acc gtg gac tac agt gtt ttc aac ccc ttc tcc tcc tcc tcc        706
Gly Asn Thr Val Asp Tyr Ser Val Phe Asn Pro Phe Ser Ser Ser Ser           
    130                 135                 140                           

tac ttt cac cca tac tgc ctc atc tcc aat tac gac aac cag acc aat        754
Tyr Phe His Pro Tyr Cys Leu Ile Ser Asn Tyr Asp Asn Gln Thr Asn           
145                 150                 155                 160           

gtt gaa gac tgc tgg ctg ggc gat acc acc gtt tcg ctg cca gat ctc        802
Val Glu Asp Cys Trp Leu Gly Asp Thr Thr Val Ser Leu Pro Asp Leu           
                165                 170                 175               

gac acg aca agc aca gcc gtg cgg aac atc tgg tac gac tgg gtg gca        850
Asp Thr Thr Ser Thr Ala Val Arg Asn Ile Trp Tyr Asp Trp Val Ala           
            180                 185                 190                   

gat ttg gtc gcc aac tat tct a gtcggtagcc cacgtcaaat ataggaaaat         902
Asp Leu Val Ala Asn Tyr Ser                                               
        195                                                               

cctcatactg acggacatag tc  gac ggt ctg cgt gtc gac act gta aaa cac      954
                      Ile Asp Gly Leu Arg Val Asp Thr Val Lys His         
                                          205                 210         

gtc gaa aaa gac ttt tgg ccc ggc tat aac agc gca gca ggc gtc tac       1002
Val Glu Lys Asp Phe Trp Pro Gly Tyr Asn Ser Ala Ala Gly Val Tyr           
                215                 220                 225               

tgt gtc ggc gag gtc tac tca ggc gat cct gca tac aca tgc ccg tac       1050
Cys Val Gly Glu Val Tyr Ser Gly Asp Pro Ala Tyr Thr Cys Pro Tyr           
            230                 235                 240                   

cag aac tac atg gac ggt gtg ctc aac tat cca at  gtaagctcct            1095
Gln Asn Tyr Met Asp Gly Val Leu Asn Tyr Pro Ile                           
        245                 250                                           

tgcctacccg gcacctattc ttatgctgac agagtttag t tac tac cag ctt ctc      1150
                                             Tyr Tyr Gln Leu Leu          
                                             255                          

tat gcg ttt gag tcg tcc agc ggc agc atc agc gat ctc tac aac atg       1198
Tyr Ala Phe Glu Ser Ser Ser Gly Ser Ile Ser Asp Leu Tyr Asn Met           
260                 265                 270                 275           

atc agc tcc gtt gcc tcc agc tgc aag gat ccc aca ctc ctg ggg aac       1246
Ile Ser Ser Val Ala Ser Ser Cys Lys Asp Pro Thr Leu Leu Gly Asn           
                280                 285                 290               

ttc atc gag aac cac gat aac ccc cgc ttc gct tc  gtgagtctta            1291
Phe Ile Glu Asn His Asp Asn Pro Arg Phe Ala Ser                           
            295                 300                                       

cctctttgct cctgcaatgc ctcactaata tctgcag c tac acg agc gac tac        1344
                                           Tyr Thr Ser Asp Tyr            
                                               305                        

tcg cag gct aag aac gtg atc acc ttc atc ttc ctg agc gac ggt atc       1392
Ser Gln Ala Lys Asn Val Ile Thr Phe Ile Phe Leu Ser Asp Gly Ile           
    310                 315                 320                           

ccc atc gtc tac gcc gga cag gaa cag cac tac agc gga ggc agc gac       1440
Pro Ile Val Tyr Ala Gly Gln Glu Gln His Tyr Ser Gly Gly Ser Asp           
325                 330                 335                 340           

cca gct aac cgc gag gcc acc tgg ctg tcc gga tac tcc acc agc gct       1488
Pro Ala Asn Arg Glu Ala Thr Trp Leu Ser Gly Tyr Ser Thr Ser Ala           
                345                 350                 355               

acg ctg tac acc tgg atc gcc acc aca aac cag atc cgc agc ctg gcg       1536
Thr Leu Tyr Thr Trp Ile Ala Thr Thr Asn Gln Ile Arg Ser Leu Ala           
            360                 365                 370                   

atc tcc aag gac gcg gga tac gtg cag gcc aag gtatacgcac gccatcccaa     1589
Ile Ser Lys Asp Ala Gly Tyr Val Gln Ala Lys                               
        375                 380                                           

tctcccacca cgctaacaca aacag aac aac ccc ttc tac tcc gac tcc aac       1641
                            Asn Asn Pro Phe Tyr Ser Asp Ser Asn           
                                385                 390                   

acc atc gcc atg cgc aag ggc acg aca gcc ggc gcg caa gtc atc acc       1689
Thr Ile Ala Met Arg Lys Gly Thr Thr Ala Gly Ala Gln Val Ile Thr           
        395                 400                 405                       

gtc ctc agc aac aag ggc gcc tcc ggc agc tcc tac act ctc tct ttg       1737
Val Leu Ser Asn Lys Gly Ala Ser Gly Ser Ser Tyr Thr Leu Ser Leu           
    410                 415                 420                           

agt ggt acc ggc tac tcc gcc ggc gcg acc ctg gtc gag acg tac acc       1785
Ser Gly Thr Gly Tyr Ser Ala Gly Ala Thr Leu Val Glu Thr Tyr Thr           
425                 430                 435                 440           

tgc acc acg gtg act gta gac tcg agc ggc aac ctg ccc gtc cca atg       1833
Cys Thr Thr Val Thr Val Asp Ser Ser Gly Asn Leu Pro Val Pro Met           
                445                 450                 455               

aca tcc ggc ttg ccg cga gtg ttt gtc ccg tcg tcc tgg gtg aat ggg       1881
Thr Ser Gly Leu Pro Arg Val Phe Val Pro Ser Ser Trp Val Asn Gly           
            460                 465                 470                   

agc gcg ctt tgc aac aca gaa tgc acg gcc gcc acg tcc atc tct gtt       1929
Ser Ala Leu Cys Asn Thr Glu Cys Thr Ala Ala Thr Ser Ile Ser Val           
        475                 480                 485                       

ctc ttc gag gaa ctg gtt acg acg acc tac ggg gag aac att tat ctg       1977
Leu Phe Glu Glu Leu Val Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu           
    490                 495                 500                           

agc ggc tcg atc agc cag ctg ggt agt tgg aat acg gcc tcg gct gtt       2025
Ser Gly Ser Ile Ser Gln Leu Gly Ser Trp Asn Thr Ala Ser Ala Val           
505                 510                 515                 520           

gcc ctg tcg gcg agt caa tat acc tcg tcc aac ccg gag tgg tat gtg       2073
Ala Leu Ser Ala Ser Gln Tyr Thr Ser Ser Asn Pro Glu Trp Tyr Val           
                525                 530                 535               

agt gtg acc ttg cct gtg ggc acg tcg ttc cag tac aag ttc atc aag       2121
Ser Val Thr Leu Pro Val Gly Thr Ser Phe Gln Tyr Lys Phe Ile Lys           
            540                 545                 550                   

aag ggg tcg gac gga agt gtt gtg tgg gag agt gat ccg aac cgg tcg       2169
Lys Gly Ser Asp Gly Ser Val Val Trp Glu Ser Asp Pro Asn Arg Ser           
        555                 560                 565                       

tat acc gtt ccg gct ggg tgc gag ggc gcg acg gtg aca gtt gct gat       2217
Tyr Thr Val Pro Ala Gly Cys Glu Gly Ala Thr Val Thr Val Ala Asp           
    570                 575                 580                           

act tgg agg tgatgctcgc cagctgaagc tgtatactct tgaacaatgt               2266
Thr Trp Arg                                                               
585                                                                       

gttcacaggc ttcatgcttg tcctctctgc ttcgggataa tctaggcagt aggaatagat     2326

tgtcgttgaa ggtc                                                       2340


<210>  2
<211>  607
<212>  PRT
<213>  Aspergillus terreus

<400>  2

Met Lys Trp Thr Ser Ser Leu Leu Leu Leu Leu Ser Val Ile Gly Gln 
-20                 -15                 -10                 -5  


Ala Thr His Ala Leu Thr Pro Ala Glu Trp Arg Ser Gln Ser Ile Tyr 
            -1  1               5                   10          


Phe Leu Leu Thr Asp Arg Phe Gly Arg Thr Asp Asn Ser Thr Thr Ala 
        15                  20                  25              


Ala Cys Asp Thr Ser Asp Arg Val Tyr Cys Gly Gly Ser Trp Gln Gly 
    30                  35                  40                  


Ile Ile Asn Gln Leu Asp Tyr Ile Gln Gly Met Gly Phe Thr Ala Ile 
45                  50                  55                  60  


Trp Ile Thr Pro Val Thr Gly Gln Phe Tyr Glu Asn Thr Gly Asp Gly 
                65                  70                  75      


Thr Ser Tyr His Gly Tyr Trp Gln Gln Asp Ile Tyr Asp Leu Asn Tyr 
            80                  85                  90          


Asn Tyr Gly Thr Ala Gln Asp Leu Lys Asn Leu Ala Asn Ala Leu His 
        95                  100                 105             


Glu Arg Gly Met Tyr Leu Met Val Asp Val Val Ala Asn His Met Gly 
    110                 115                 120                 


Tyr Asp Gly Ala Gly Asn Thr Val Asp Tyr Ser Val Phe Asn Pro Phe 
125                 130                 135                 140 


Ser Ser Ser Ser Tyr Phe His Pro Tyr Cys Leu Ile Ser Asn Tyr Asp 
                145                 150                 155     


Asn Gln Thr Asn Val Glu Asp Cys Trp Leu Gly Asp Thr Thr Val Ser 
            160                 165                 170         


Leu Pro Asp Leu Asp Thr Thr Ser Thr Ala Val Arg Asn Ile Trp Tyr 
        175                 180                 185             


Asp Trp Val Ala Asp Leu Val Ala Asn Tyr Ser Ile Asp Gly Leu Arg 
    190                 195                 200                 


Val Asp Thr Val Lys His Val Glu Lys Asp Phe Trp Pro Gly Tyr Asn 
205                 210                 215                 220 


Ser Ala Ala Gly Val Tyr Cys Val Gly Glu Val Tyr Ser Gly Asp Pro 
                225                 230                 235     


Ala Tyr Thr Cys Pro Tyr Gln Asn Tyr Met Asp Gly Val Leu Asn Tyr 
            240                 245                 250         


Pro Ile Tyr Tyr Gln Leu Leu Tyr Ala Phe Glu Ser Ser Ser Gly Ser 
        255                 260                 265             


Ile Ser Asp Leu Tyr Asn Met Ile Ser Ser Val Ala Ser Ser Cys Lys 
    270                 275                 280                 


Asp Pro Thr Leu Leu Gly Asn Phe Ile Glu Asn His Asp Asn Pro Arg 
285                 290                 295                 300 


Phe Ala Ser Tyr Thr Ser Asp Tyr Ser Gln Ala Lys Asn Val Ile Thr 
                305                 310                 315     


Phe Ile Phe Leu Ser Asp Gly Ile Pro Ile Val Tyr Ala Gly Gln Glu 
            320                 325                 330         


Gln His Tyr Ser Gly Gly Ser Asp Pro Ala Asn Arg Glu Ala Thr Trp 
        335                 340                 345             


Leu Ser Gly Tyr Ser Thr Ser Ala Thr Leu Tyr Thr Trp Ile Ala Thr 
    350                 355                 360                 


Thr Asn Gln Ile Arg Ser Leu Ala Ile Ser Lys Asp Ala Gly Tyr Val 
365                 370                 375                 380 


Gln Ala Lys Asn Asn Pro Phe Tyr Ser Asp Ser Asn Thr Ile Ala Met 
                385                 390                 395     


Arg Lys Gly Thr Thr Ala Gly Ala Gln Val Ile Thr Val Leu Ser Asn 
            400                 405                 410         


Lys Gly Ala Ser Gly Ser Ser Tyr Thr Leu Ser Leu Ser Gly Thr Gly 
        415                 420                 425             


Tyr Ser Ala Gly Ala Thr Leu Val Glu Thr Tyr Thr Cys Thr Thr Val 
    430                 435                 440                 


Thr Val Asp Ser Ser Gly Asn Leu Pro Val Pro Met Thr Ser Gly Leu 
445                 450                 455                 460 


Pro Arg Val Phe Val Pro Ser Ser Trp Val Asn Gly Ser Ala Leu Cys 
                465                 470                 475     


Asn Thr Glu Cys Thr Ala Ala Thr Ser Ile Ser Val Leu Phe Glu Glu 
            480                 485                 490         


Leu Val Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Ser Gly Ser Ile 
        495                 500                 505             


Ser Gln Leu Gly Ser Trp Asn Thr Ala Ser Ala Val Ala Leu Ser Ala 
    510                 515                 520                 


Ser Gln Tyr Thr Ser Ser Asn Pro Glu Trp Tyr Val Ser Val Thr Leu 
525                 530                 535                 540 


Pro Val Gly Thr Ser Phe Gln Tyr Lys Phe Ile Lys Lys Gly Ser Asp 
                545                 550                 555     


Gly Ser Val Val Trp Glu Ser Asp Pro Asn Arg Ser Tyr Thr Val Pro 
            560                 565                 570         


Ala Gly Cys Glu Gly Ala Thr Val Thr Val Ala Asp Thr Trp Arg 
        575                 580                 585         


<210>  3
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is glu or asp

<400>  3

Ile Xaa Gly Arg 
1               


<210>  4
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<400>  4

Asp Asp Asp Asp Lys 
1               5   


<210>  5
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<400>  5

Leu Val Pro Arg Gly Ser 
1               5       


<210>  6
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<400>  6

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  7
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<400>  7

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


