                         SEQUENCE LISTING

<110>  Mymetics Corporation
 
<120>  Novel gp41 antigens

<130>  BR066824

<150>  61/202 215
<151>  2009-02-06

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  1

Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
1               5                   10                  15      


Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
            20                  25                  30          


Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu 
        35                  40                  45              


Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile 
    50                  55                  60                  


Cys Thr Thr Ala Val Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu 
65                  70                  75                  80  


Glu Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile 
                85                  90                  95      


Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn 
            100                 105                 110         


Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala 
        115                 120                 125             


Ser Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr 
    130                 135                 140         


<210>  2
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 clade B without S

<400>  2

Ser Gln Thr Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
1               5                   10                  15      


Lys Trp Ala Ser Leu Trp Asn Trp Phe Asp Ile Thr Asn Trp Leu Trp 
            20                  25                  30          


Tyr Ile Lys 
        35  


<210>  3
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 clade C type without spacer

<400>  3

Ser Gln Thr Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Ala Leu Asp 
1               5                   10                  15      


Ser Trp Lys Asn Leu Trp Asn Trp Phe Ser Ile Thr Asn Trp Leu Trp 
            20                  25                  30          


Tyr Ile Lys 
        35  


<210>  4
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 clade B with S LGC

<400>  4

Ser Gln Thr Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
1               5                   10                  15      


Lys Trp Ala Ser Leu Trp Asn Trp Phe Asp Ile Thr Asn Trp Leu Trp 
            20                  25                  30          


Tyr Ile Lys Leu Gly Cys 
        35              


<210>  5
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 clade B with S LSC

<400>  5

Ser Gln Thr Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
1               5                   10                  15      


Lys Trp Ala Ser Leu Trp Asn Trp Phe Asp Ile Thr Asn Trp Leu Trp 
            20                  25                  30          


Tyr Ile Lys Leu Ser Cys 
        35              


<210>  6
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 clade C type with S

<400>  6

Ser Gln Thr Gln Gln Glu Lys Asn Glu Gln Leu Leu Ala Leu Asp Ser 
1               5                   10                  15      


Trp Lys Asn Leu Trp Asn Trp Phe Ser Ile Thr Asn Trp Leu Trp Tyr 
            20                  25                  30          


Ile Lys Leu Gly Cys 
        35          


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2F5 epitope

<400>  7

Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn 
1               5                   10                  15      


<210>  8
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calveolin binding domain

<400>  8

Ser Leu Glu Gln Ile Trp Asn Asn Met Thr Trp Met Gln Trp Asp Lys 
1               5                   10                  15      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer

<400>  9

Leu Glu His Ser His His His 
1               5           


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer with cysteine

<400>  10

Leu Glu His Ser His His His Cys 
1               5               


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer

<400>  11

Leu Glu His His His His 
1               5       


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer with cysteine

<400>  12

Leu Glu His His His His Cys 
1               5           


<210>  13
<211>  54
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu 
    50                  


<210>  14
<211>  54
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Asp Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu 
    50                  


<210>  15
<211>  47
<212>  PRT
<213>  Homo sapiens

<400>  15

Ser Leu Glu Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg 
1               5                   10                  15      


Glu Ile Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser 
            20                  25                  30          


Gln Asn Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
        35                  40                  45          


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  16

Ser Gly Gly Arg Gly Gly Ser 
1               5           


<210>  17
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Ser Gly Gly Arg Gly Gly Ser Ser Leu Glu 
    50                  55                  60                  


Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
65                  70                  75                  80  


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
                85                  90                  95      


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
            100                 105             


<210>  18
<211>  108
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Asp Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Ser Gly Gly Arg Gly Gly Ser Ser Leu Glu 
    50                  55                  60                  


Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
65                  70                  75                  80  


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
                85                  90                  95      


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
            100                 105             


<210>  19
<211>  115
<212>  PRT
<213>  Homo sapiens

<400>  19

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Ser Gly Gly Arg Gly Gly Ser Ser Leu Glu 
    50                  55                  60                  


Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
65                  70                  75                  80  


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
                85                  90                  95      


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Leu Glu His His 
            100                 105                 110         


His His Cys 
        115 


<210>  20
<211>  116
<212>  PRT
<213>  Homo sapiens

<400>  20

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Ser Gly Gly Arg Gly Gly Ser Ser Leu Glu 
    50                  55                  60                  


Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
65                  70                  75                  80  


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
                85                  90                  95      


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Leu Glu His Ser 
            100                 105                 110         


His His His Cys 
        115     


<210>  21
<211>  348
<212>  DNA
<213>  Homo sapiens

<400>  21
atgcaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct       60

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca      120

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcagtggagg tagaggtgga      180

tcctctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac      240

aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat      300

gaacaagaat tattggaatt agatctggaa cattctcatc accactgc                   348


<210>  22
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41 Nde1

<400>  22
ggaatccaca tatgcaggcc agacaattat tg                                     32


<210>  23
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41-Bam1

<400>  23
accgttggat ccacctctac ctccactgag ctgttgatcc tttaggtatc                  50


<210>  24
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41-Bam2

<400>  24
ggaatccagg atcctctctg gaacagattt ggaatcac                               38


<210>  25
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41-Xho1

<400>  25
gcccggctcg agatctaatt ccaataattc ttgttcattc ttttc                       45


<210>  26
<211>  411
<212>  DNA
<213>  Homo sapiens

<400>  26
atgcaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct       60

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca      120

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggtagc      180

tctggaaaac tcattagcac cactgctgtg ccttggaatg ctagttggag taataaatct      240

ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac      300

acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa      360

gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat a               411


<210>  27
<211>  330
<212>  DNA
<213>  Homo sapiens

<400>  27
atgcaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct       60

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca      120

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcagtggagg tagaggtgga      180

tcctctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac      240

aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat      300

gaacaagaat tattggaatt agatctcgag                                       330


<210>  28
<211>  345
<212>  DNA
<213>  Homo sapiens

<400>  28
atgcaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct       60

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca      120

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcagtggagg tagaggtgga      180

tcctctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac      240

aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat      300

gaacaagaat tattggaatt agatctggaa catcatcacc actgc                      345


<210>  29
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prime gp41B-C-D1

<400>  29
taattccata tgcaggccag acaattattg tctg                                   34


<210>  30
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41B-C-R2

<400>  30
attccgctcg agttattagc agtggtgatg agaatgttcc ag                          42


<210>  31
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41B-C-R1

<400>  31
gtgatgagaa tgttccagat ctaattccaa taattcttgt tcatt                       45


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T7 prom

<400>  32
taatacgact cactataggg                                                   20


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T7 term

<400>  33
gctagttatt gctcagcgg                                                    19


<210>  34
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41B-C3-R1

<400>  34
gtgatgagaa tgttccagat ctaattccaa taattcttgt tcatt                       45


<210>  35
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer gp41B-C3-R2

<400>  35
attccgctcg agttattagc agtggtgatg agaatgttcc ag                          42


<210>  36
<211>  390
<212>  DNA
<213>  Homo sapiens

<400>  36
atgcaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct       60

attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca      120

agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat tgacggtagc      180

agtggaggta gaggtggatc caatgctagt tggagtaata aatctctgga acagatttgg      240

aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac      300

tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta      360

gatctcgagc accaccacca ccaccactga                                       390


<210>  37
<211>  129
<212>  PRT
<213>  Homo sapiens

<400>  37

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Leu Gly Ile Asp Gly Ser Ser Gly Gly Arg 
    50                  55                  60                  


Gly Gly Ser Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp 
65                  70                  75                  80  


Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr 
                85                  90                  95      


Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys 
            100                 105                 110         


Asn Glu Gln Glu Leu Leu Glu Leu Asp Leu Glu His His His His His 
        115                 120                 125             


His 
    


<210>  38
<211>  110
<212>  PRT
<213>  Homo sapiens

<400>  38

Met Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn 
1               5                   10                  15      


Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val 
            20                  25                  30          


Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
        35                  40                  45              


Leu Lys Asp Gln Gln Leu Ser Gly Gly Arg Gly Gly Ser Ser Leu Glu 
    50                  55                  60                  


Gln Ile Trp Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
65                  70                  75                  80  


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
                85                  90                  95      


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Leu Glu 
            100                 105                 110 


