                         SEQUENZPROTOKOLL

<110>  Technische Universitt Dresden
 
<120>  Autokatalytischer Proteinlinker und seine Verwendung zur 
       Herstellung von Fusionsproteinen

<130>  00017P0123DEWO

<150>  DE 10 2009 008 252
<151>  2009-02-02

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  170
<212>  PRT
<213>  Bradyrhizobium japonicum

<400>  1

Trp Thr His Glu Val Lys Asp Gly Lys Ala Thr Ile Asn Leu Gly Asp 
1               5                   10                  15      


Lys Tyr Thr Ile Thr Ala Asn Glu Lys Asp Gly Thr Trp Thr Val Arg 
            20                  25                  30          


Asn Asn Gln Thr Gly His Val Thr Val Ile His Gly Asp Pro His Val 
        35                  40                  45              


Asp Ala Asp Gly Asp Gly Lys Asp Asp Phe Asp Phe Lys Lys Asp Met 
    50                  55                  60                  


Thr Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asn Asn Val Asp 
65                  70                  75                  80  


Tyr Gly Asn Gly Gln Ala Ile Ser Ser Lys Leu Thr Ile Thr Asn Gly 
                85                  90                  95      


His Asn Ala Ile Val Val Glu Gly Leu Gly Asp Asp Lys Asp Gly Lys 
            100                 105                 110         


Asn Asn Leu Arg Val Thr Gln Ser Asn Ala Gly Met Thr Leu Asp Glu 
        115                 120                 125             


Leu Thr Ser Asp Gly Ala Gln Thr Ile His Glu Ser Gly Gln Gly Trp 
    130                 135                 140                 


Val Asp Gly Ala Gly Arg Ala Val Asn Gln Ala Ser Ile Asp Asp Gly 
145                 150                 155                 160 


Glu Gln Gly Arg Gly Pro Gly Asn Tyr Gln 
                165                 170 


<210>  2
<211>  170
<212>  PRT
<213>  Bradyrhizobium japonicum

<400>  2

Trp Ser His Glu Val Arg Asp Gly Lys Ala Glu Ile Lys Leu Gly Asp 
1               5                   10                  15      


Lys Tyr Ser Ile Leu Val Asp Glu Asn Asp Gly Thr Val Leu Ile Arg 
            20                  25                  30          


Asn Ser Gln Thr Gly Lys Ile Thr Ser Ile Lys Gly Asp Pro His Val 
        35                  40                  45              


Asp Ala Asp Gly Asp Gly Lys Val Asp Phe Asp Phe Lys Lys Asn Met 
    50                  55                  60                  


Thr Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asp Thr Val Asp 
65                  70                  75                  80  


Ile Gly Lys Gly Lys Thr Met Ala Ser Lys Leu Thr Ile Thr Asn Gly 
                85                  90                  95      


Asp Asn Ala Met Val Val Glu Gly Leu Gly Asp Arg Phe Asp Gly Lys 
            100                 105                 110         


Asn Asn Leu Lys Val Thr Gln Ser Asn Ala Gly Arg Thr Leu Asp Gln 
        115                 120                 125             


Leu Thr Ser Asp Gly Ala Gln Thr Ile Tyr Glu Gln Pro Gly Ser Gly 
    130                 135                 140                 


Trp Val Asp Arg Ser Gly Arg Gln Val Asn Gln Glu Ile Ile Asp Ser 
145                 150                 155                 160 


Asn Glu Asn Pro Gly Thr Thr Ser Asp Ala 
                165                 170 


<210>  3
<211>  170
<212>  PRT
<213>  Bradyrhizobium japonicum

<400>  3

Trp Thr His Glu Val Lys Asp Gly Lys Ala Thr Ile Asn Leu Gly Asp 
1               5                   10                  15      


Lys Tyr Thr Ile Thr Ala Asn Glu Lys Asp Gly Thr Trp Thr Val Arg 
            20                  25                  30          


Asn Asn Gln Thr Gly His Val Thr Lys Ile His Gly Asp Pro His Val 
        35                  40                  45              


Asp Ala Asn Gly Asp Gly Lys Asp Asp Phe Asp Phe Lys Lys Asp Met 
    50                  55                  60                  


Thr Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asn Asn Val Asp 
65                  70                  75                  80  


Tyr Gly Asn Gly Glu Thr Ile Ser Ser Arg Leu Thr Ile Thr Asn Gly 
                85                  90                  95      


His Asn Ala Met Val Val Glu Gly Leu Gly Asp Asp Lys Asp Gly Lys 
            100                 105                 110         


Asn Asn Leu Arg Val Thr Gln Ser Asn Ala Gly Met Thr Leu Asp Glu 
        115                 120                 125             


Leu Thr Pro Asp Gly Ala Gln Thr Ile His Glu Ser Gly Gln Gly Trp 
    130                 135                 140                 


Val Asp Gly Ala Gly Gly Val Val Asn Gln Ala Ser Ile Asp Ala Gly 
145                 150                 155                 160 


Glu Gln Gly Arg Ala Pro Gly Asn Tyr Gln 
                165                 170 


<210>  4
<211>  170
<212>  PRT
<213>  Bradyrhizobium japonicum

<400>  4

Trp Ser His Glu Val Lys Asp Gly Lys Ala Thr Ile Asn Leu Gly Asp 
1               5                   10                  15      


Lys Tyr Thr Ile Thr Ala Asp Glu Lys Asp Gly Thr Trp Thr Val Arg 
            20                  25                  30          


Asn Asn Gln Thr Gly His Val Ser Arg Ile His Gly Asp Pro His Val 
        35                  40                  45              


Asp Ala Asn Gly Asp Gly Lys Asp Asp Phe Asp Phe Lys Lys Gly Met 
    50                  55                  60                  


Thr Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asp Thr Val Asn 
65                  70                  75                  80  


Tyr Gly Lys Gly Lys Thr Ile Ser Ser Lys Leu Thr Ile Thr Asn Gly 
                85                  90                  95      


Asp Asn Ala Met Val Val Glu Gly Leu Gly Asp Asp Lys Asp Gly Lys 
            100                 105                 110         


Asn Asn Leu Lys Val Thr Gln Ser Asn Ala Gly Arg Thr Leu Asp Gln 
        115                 120                 125             


Leu Thr Ser Asp Gly Ala Gln Thr Ile His Glu Thr Asn Gln Gly Trp 
    130                 135                 140                 


Val Asp Asn Ser Gly Arg Lys Val Thr Gln Ser Val Ile Asn His Asn 
145                 150                 155                 160 


Glu Asn Pro Asp Ala Pro Pro Asp Phe Lys 
                165                 170 


<210>  5
<211>  156
<212>  PRT
<213>  Burkholderia phytofirmans

<400>  5

Trp Ser Asn Thr Gln Val Asn Asp Asn Lys Ser Thr Ile Asp Leu Gly 
1               5                   10                  15      


Asn Tyr Lys Leu Asp Leu Asn Lys Lys Asp Ser Ser Met Leu Leu Thr 
            20                  25                  30          


Asp Lys Lys Ser Gly Glu Thr Thr Lys Val Trp Gly Asp Pro His Ile 
        35                  40                  45              


Asp Ser Asn Gly Thr Ser Asn Met Phe Asn Gly Pro Leu Ser Leu Asn 
    50                  55                  60                  


Leu Ser Asp Gly Thr Lys Ile Thr Val Gly Thr Gln Gly Lys Gly Asn 
65                  70                  75                  80  


Val Ser Tyr Ala Asp Lys Leu Thr Ile Thr Lys Gly Asn Asp Ala Tyr 
                85                  90                  95      


Leu Val Asn Gly Leu Ser Glu Lys Asp Ser Asn Pro Leu Thr Val Gln 
            100                 105                 110         


His Ala Gly Asn Gly Arg Gln Leu Asp Ala Met Thr Pro Asp Gly Tyr 
        115                 120                 125             


Ser Leu Val Ala Asn Gln Asn Gly Lys Gly Trp Ile Asp Pro Gln Thr 
    130                 135                 140                 


Gly His Ala Pro Thr Ala Ala Asp Phe Lys Lys His 
145                 150                 155     


<210>  6
<211>  171
<212>  PRT
<213>  Sphingomonas sp. S88

<400>  6

Ser Ala Ser Met Glu Gly Glu Ser Gln Ala Ser Ile Asp Leu Gly Asp 
1               5                   10                  15      


Gly Tyr Ser Leu Glu Leu Asp Glu Arg Asn Ser Glu Ile Arg Ile Tyr 
            20                  25                  30          


Asn Ala Ser Thr Gly Glu Thr Thr Arg Val Trp Gly Asp Pro His Val 
        35                  40                  45              


Asp Ile Asp Gly Lys His Ala Phe Asp Phe Trp Gly Thr Thr Ser Phe 
    50                  55                  60                  


Glu Leu Glu Asn Gly Thr Lys Ile Thr Ile Asp Thr Glu Gln Tyr Ala 
65                  70                  75                  80  


Ala Asn Pro Asn Glu Tyr Leu Ala Ser Lys Leu Thr Ile Thr Lys Gly 
                85                  90                  95      


Asp Gln Ala Ile Val Val Asp Gly Ile Ser Gln Asn Gln Ile Gly Asp 
            100                 105                 110         


Leu Ser Ile Thr Gln Ser Asn Asn Gly Gln Met Leu Asp Met Leu Thr 
        115                 120                 125             


Arg Asp Gly Phe Val Leu Gln Glu Asn Ala Thr Gly Ala Gly Trp Arg 
    130                 135                 140                 


Ser Glu Leu Thr Gly Asp Ile Ala Thr Gln Ala Asp Leu Asn Leu Thr 
145                 150                 155                 160 


Lys Val Gly Ala Ile Tyr Gly Pro Gly Ser Glu 
                165                 170     


<210>  7
<211>  148
<212>  PRT
<213>  Vibrio coralliilyticus BAA450

<400>  7

Ser Tyr Glu Pro Ser Ser Gly Lys Ala Thr Leu Glu Asn Asp Arg Tyr 
1               5                   10                  15      


Thr Ile Asn Ile Asp Glu Ser Ser Ser Glu Ile Glu Val Ile Asp Lys 
            20                  25                  30          


Gln Asn Pro Glu Asp Ser Phe Arg Ile Tyr Gly Asp Pro His Phe Asp 
        35                  40                  45              


Ile Gly Asn Asp Gly Asp Thr Asp Phe Asp Phe Lys Lys Asp Met Ser 
    50                  55                  60                  


Ile Glu Leu Asp Asp Gly Thr Lys Leu His Ile His Thr Thr Pro Thr 
65                  70                  75                  80  


Ser Asn Gly Glu Thr Leu Ala Thr Ser Leu Ala Ile Glu Glu Pro Asp 
                85                  90                  95      


Gly Ser Gly Trp Tyr Ile Glu Gly Ile Asp Ser Asp Gln Lys Gly Asp 
            100                 105                 110         


Leu Glu Val Lys Glu Tyr Asn Asn Ile Asn Tyr Ser Gly Gly Thr Val 
        115                 120                 125             


Asn Asp Asp Ala Ala Leu Glu Leu Gln Val Arg Asp Gly Asn Tyr Phe 
    130                 135                 140                 


Leu Asn Ser Asp 
145             


<210>  8
<211>  117
<212>  PRT
<213>  Myxococcus xanthus DK1622

<400>  8

Asn Ser Leu Lys Val Glu Glu Gly Thr Gly Lys Ile Thr Thr Pro Gly 
1               5                   10                  15      


Gly Tyr Thr Ile Glu Gln Leu Gly Gln Phe Glu Trp Arg Val Thr Gly 
            20                  25                  30          


Pro Asp Gly Lys Asn Thr Arg Val Trp Gly Asp Pro His Val Asp Glu 
        35                  40                  45              


Ser Asp Gly Gly Lys Phe Asp Phe Lys Arg Asp Thr Ser Phe Val Leu 
    50                  55                  60                  


Gly Asp Gly Thr Arg Ile Asn Cys Ser Cys Lys Pro Trp Gly Asn Gly 
65                  70                  75                  80  


Met Thr Val Thr Gly Gln Leu Asp Val Val Tyr Gly Asp Ser His Val 
                85                  90                  95      


Arg Val Thr Asp Ile Asp Lys Gly Lys Gly Lys Val Gly Gln Val Thr 
            100                 105                 110         


Asn Lys Gly Met Asp 
        115         


<210>  9
<211>  15
<212>  PRT
<213>  Unknown

<220>
<223>  EF-Hand like motif


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Val, Ile or Phe

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Ala, Ser, Ile or Glu

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be Asp, Asn, Gly or Ser

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Gly or Asn

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be Gly or Asp

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be Lys, Thr, Asp or Gly

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be Asp, Val, Ser, His, Thr or Gly

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be Asp, Asn, Ala or Lys
<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be Phe or Met

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Xaa can be Lys, Asn or Trp

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Xaa can be Lys, Gly or Arg

<400>  9

Xaa Asp Xaa Xaa Xaa Asp Xaa Xaa Xaa Xaa Xaa Asp Phe Xaa Xaa 
1               5                   10                  15  


<210>  10
<211>  12
<212>  PRT
<213>  Unknown

<220>
<223>  Consensus Sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Thr or Ser

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be Phe, Leu or Ile

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Gln, Asn, Glu or Val

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Asp, Ser, Glu or Gly

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be Asp or Asn

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be Lys or Arg

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be Ile or Leu

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be Thr, His or Asn

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Xaa can be Val, Ile or Cys

<400>  10

Xaa Xaa Xaa Leu Xaa Xaa Gly Thr Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  11
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  Consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Lys, Arg, Ser or Gln

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Thr, Ala or Asp

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be Ile or Val

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Thr, Glu or Val

<400>  11

Xaa Leu Xaa Xaa Xaa 
1               5   


<210>  12
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  Consensus Sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Val or Ile

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be Glu, Asn, Asp or Thr

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Gly or Asp

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be Leu or Ile

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Gly, Ser or Asp

<400>  12

Xaa Xaa Xaa Xaa Xaa 
1               5   


<210>  13
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  Consensus sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Thr or Asn

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be Ser, Pro, Arg or Tyr

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be Asp or Ser

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Ala, Tyr, Phe or Gly

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be Gln, Ser, Val or Thr

<400>  13

Xaa Xaa Xaa Gly Xaa Xaa 
1               5       


<210>  14
<211>  713
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GST-Blr1806-Fusionsprotein

<400>  14

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg 
    210                 215                 220                 


Gly Ser Pro Asn Ser Met Gln Tyr Leu Pro Val Ala Gly Leu Pro Val 
225                 230                 235                 240 


Val Gly Ala Pro Asp Ser Met Asn Gly Val Ala Pro Glu Gly Ala Val 
                245                 250                 255     


Val Thr Pro Thr Phe Asn Ala Met Leu Gly Gln Tyr Ala Pro Ala Ser 
            260                 265                 270         


Tyr Gln Tyr Leu Pro Val Ala Ser Pro Ala Leu Val Gly Thr Ala Val 
        275                 280                 285             


Gly Ser Val Val Pro Val Val Val Ala Pro Ala Val Val Thr Thr Gln 
    290                 295                 300                 


Ala Tyr Met Met Ala Pro Pro Pro Ser Thr Met Leu Met Ser Gln Leu 
305                 310                 315                 320 


Asn Gln Asn Ala Glu Pro Pro Val Asp Pro Val Trp Thr His Glu Val 
                325                 330                 335     


Lys Asp Gly Lys Ala Thr Ile Asn Leu Gly Asp Lys Tyr Thr Ile Thr 
            340                 345                 350         


Ala Asn Glu Lys Asp Gly Thr Trp Thr Val Arg Asn Asn Gln Thr Gly 
        355                 360                 365             


His Val Thr Val Ile His Gly Asp Pro His Val Asp Ala Asp Gly Asp 
    370                 375                 380                 


Gly Lys Asp Asp Phe Asp Phe Lys Lys Asp Met Thr Phe Gln Leu Asp 
385                 390                 395                 400 


Asp Gly Thr Lys Ile Thr Val Asn Asn Val Asp Tyr Gly Asn Gly Gln 
                405                 410                 415     


Ala Ile Ser Ser Lys Leu Thr Ile Thr Asn Gly His Asn Ala Ile Val 
            420                 425                 430         


Val Glu Gly Leu Gly Asp Asp Lys Asp Gly Lys Asn Asn Leu Arg Val 
        435                 440                 445             


Thr Gln Ser Asn Ala Gly Met Thr Leu Asp Glu Leu Thr Ser Asp Gly 
    450                 455                 460                 


Ala Gln Thr Ile His Glu Ser Gly Gln Gly Trp Val Asp Gly Ala Gly 
465                 470                 475                 480 


Arg Ala Val Asn Gln Ala Ser Ile Asp Asp Gly Glu Gln Gly Arg Gly 
                485                 490                 495     


Pro Gly Asn Tyr Gln Tyr Ala Ser Gly Ala Pro Thr Ser Thr Ala Pro 
            500                 505                 510         


Val Phe Phe Val Pro Pro Pro Pro Pro Pro Leu Ala Met Val Pro Val 
        515                 520                 525             


Ala Ala Ala Gln Val Pro Thr Gln Ser Ser Gln Pro Ala Pro Val Trp 
    530                 535                 540                 


Ser His Glu Val Arg Asp Gly Lys Ala Glu Ile Lys Leu Gly Asp Lys 
545                 550                 555                 560 


Tyr Ser Ile Leu Val Asp Glu Asn Asp Gly Thr Val Leu Ile Arg Asn 
                565                 570                 575     


Ser Gln Thr Gly Lys Ile Thr Ser Ile Lys Gly Asp Pro His Val Asp 
            580                 585                 590         


Ala Asp Gly Asp Gly Lys Val Asp Phe Asp Phe Lys Lys Asn Met Thr 
        595                 600                 605             


Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asp Thr Val Asp Ile 
    610                 615                 620                 


Gly Lys Gly Lys Thr Met Ala Ser Lys Leu Thr Ile Thr Asn Gly Asp 
625                 630                 635                 640 


Asn Ala Met Val Val Glu Gly Leu Gly Asp Arg Phe Asp Gly Lys Asn 
                645                 650                 655     


Asn Leu Lys Val Thr Gln Ser Asn Ala Gly Arg Thr Leu Asp Gln Leu 
            660                 665                 670         


Thr Ser Asp Gly Ala Gln Thr Ile Tyr Glu Gln Pro Gly Ser Gly Trp 
        675                 680                 685             


Val Asp Arg Ser Gly Arg Gln Val Asn Gln Glu Ile Ile Asp Ser Asn 
    690                 695                 700                 


Glu Asn Pro Gly Thr Thr Ser Asp Ala 
705                 710             


<210>  15
<211>  740
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GST-Blr1649-Fusionsprotein

<400>  15

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg 
    210                 215                 220                 


Gly Ser Pro Asn Ser Ala Tyr Leu Pro Val Ala Gly Leu Pro Val Val 
225                 230                 235                 240 


Gly Ala Leu Asp Ser Thr Val Asn Gly Val Gly Ser Glu Gly Val Val 
                245                 250                 255     


Ala Ser Pro Ala Phe Ser Thr Leu Leu Gly Gln Tyr Val Thr Pro Gly 
            260                 265                 270         


Ala Tyr Gln Tyr Leu Pro Ala Gly Ala Ala Val Val Val Ala Pro Val 
        275                 280                 285             


Val Val Pro Ala Gly Gly Val Thr Thr Ala Val Val Ala Pro Ala Val 
    290                 295                 300                 


Val Thr Pro Val Val Ala Ala Pro Val Tyr Met Met Ala Pro Pro Pro 
305                 310                 315                 320 


Pro Phe Met Leu Leu Ser Gln Gln Ser Ser Gly Ser Glu Pro Ser Val 
                325                 330                 335     


Asp Pro Val Trp Thr His Glu Val Lys Asp Gly Lys Ala Thr Ile Asn 
            340                 345                 350         


Leu Gly Asp Lys Tyr Thr Ile Thr Ala Asn Glu Lys Asp Gly Thr Trp 
        355                 360                 365             


Thr Val Arg Asn Asn Gln Thr Gly His Val Thr Lys Ile His Gly Asp 
    370                 375                 380                 


Pro His Val Asp Ala Asn Gly Asp Gly Lys Asp Asp Phe Asp Phe Lys 
385                 390                 395                 400 


Lys Asp Met Thr Phe Gln Leu Asp Asp Gly Thr Lys Ile Thr Val Asn 
                405                 410                 415     


Asn Val Asp Tyr Gly Asn Gly Glu Thr Ile Ser Ser Arg Leu Thr Ile 
            420                 425                 430         


Thr Asn Gly His Asn Ala Met Val Val Glu Gly Leu Gly Asp Asp Lys 
        435                 440                 445             


Asp Gly Lys Asn Asn Leu Arg Val Thr Gln Ser Asn Ala Gly Met Thr 
    450                 455                 460                 


Leu Asp Glu Leu Thr Pro Asp Gly Ala Gln Thr Ile His Glu Ser Gly 
465                 470                 475                 480 


Gln Gly Trp Val Asp Gly Ala Gly Gly Val Val Asn Gln Ala Ser Ile 
                485                 490                 495     


Asp Ala Gly Glu Gln Gly Arg Ala Pro Gly Asn Tyr Gln Ser Ala Ser 
            500                 505                 510         


Ala Thr Pro Ala Ser Ala Thr Pro Val Phe Phe Val Pro Pro Pro Pro 
        515                 520                 525             


Pro Pro Leu Ala Met Val Pro Phe Ala Val Ala Gln Thr Pro Ala Pro 
    530                 535                 540                 


Ser Ser Gln Pro Ala Pro Val Trp Ser His Glu Val Lys Asp Gly Lys 
545                 550                 555                 560 


Ala Thr Ile Asn Leu Gly Asp Lys Tyr Thr Ile Thr Ala Asp Glu Lys 
                565                 570                 575     


Asp Gly Thr Trp Thr Val Arg Asn Asn Gln Thr Gly His Val Ser Arg 
            580                 585                 590         


Ile His Gly Asp Pro His Val Asp Ala Asn Gly Asp Gly Lys Asp Asp 
        595                 600                 605             


Phe Asp Phe Lys Lys Gly Met Thr Phe Gln Leu Asp Asp Gly Thr Lys 
    610                 615                 620                 


Ile Thr Val Asp Thr Val Asn Tyr Gly Lys Gly Lys Thr Ile Ser Ser 
625                 630                 635                 640 


Lys Leu Thr Ile Thr Asn Gly Asp Asn Ala Met Val Val Glu Gly Leu 
                645                 650                 655     


Gly Asp Asp Lys Asp Gly Lys Asn Asn Leu Lys Val Thr Gln Ser Asn 
            660                 665                 670         


Ala Gly Arg Thr Leu Asp Gln Leu Thr Ser Asp Gly Ala Gln Thr Ile 
        675                 680                 685             


His Glu Thr Asn Gln Gly Trp Val Asp Asn Ser Gly Arg Lys Val Thr 
    690                 695                 700                 


Gln Ser Val Ile Asn His Asn Glu Asn Pro Asp Ala Pro Pro Asp Phe 
705                 710                 715                 720 


Lys Thr Met Met Arg Glu Ala Met Phe Arg Arg Ile Phe Gly Arg Asp 
                725                 730                 735     


Ala Gln Pro Ala 
            740 


