                         SEQUENCE LISTING

<110>  The Brigham and Women's Hospital
       University of Washington
       Case Western Reserve University
 
<120>  Secreted Phospholipase A2 Biomarkers for Arthritis

<130>  0092662-0060

<150>  US 61/146867
<151>  2009-01-23

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  997
<212>  DNA
<213>  Homo sapiens

<400>  1
gaaggaaaaa gagcaacaga tccagggagc attcacctgc cctgtctcca aacagccttg       60

tgcctcacct acccccaacc tcccagaggg agcagctatt taaggggagc aggagtgcag      120

aacaaacaag acggcctggg gatacaactc tggagtcctc tgagagagcc accaaggagg      180

agcaggggag cgacggccgg ggcagaagtt gagaccaccc agcagaggag ctaggccagt      240

ccatctgcat ttgtcaccca agaactctta ccatgaagac cctcctactg ttggcagtga      300

tcatgatctt tggcctactg caggcccatg ggaatttggt gaatttccac agaatgatca      360

agttgacgac aggaaaggaa gccgcactca gttatggctt ctacggctgc cactgtggcg      420

tgggtggcag aggatccccc aaggatgcaa cggatcgctg ctgtgtcact catgactgtt      480

gctacaaacg tctggagaaa cgtggatgtg gcaccaaatt tctgagctac aagtttagca      540

actcggggag cagaatcacc tgtgcaaaac aggactcctg cagaagtcaa ctgtgtgagt      600

gtgataaggc tgctgccacc tgttttgcta gaaacaagac gacctacaat aaaaagtacc      660

agtactattc caataaacac tgcagaggga gcacccctcg ttgctgagtc ccctcttccc      720

tggaaacctt ccacccagtg ctgaatttcc ctctctcata ccctccctcc ctaccctaac      780

caagttcctt ggccatgcag aaagcatccc tcacccatcc tagaggccag gcaggagccc      840

ttctataccc acccagaatg agacatccag cagatttcca gccttctact gctctcctcc      900

acctcaactc cgtgcttaac caaagaagct gtactccggg gggtctcttc tgaataaagc      960

aattagcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa                               997


<210>  2
<211>  144
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Lys Thr Leu Leu Leu Leu Ala Val Ile Met Ile Phe Gly Leu Leu 
1               5                   10                  15      


Gln Ala His Gly Asn Leu Val Asn Phe His Arg Met Ile Lys Leu Thr 
            20                  25                  30          


Thr Gly Lys Glu Ala Ala Leu Ser Tyr Gly Phe Tyr Gly Cys His Cys 
        35                  40                  45              


Gly Val Gly Gly Arg Gly Ser Pro Lys Asp Ala Thr Asp Arg Cys Cys 
    50                  55                  60                  


Val Thr His Asp Cys Cys Tyr Lys Arg Leu Glu Lys Arg Gly Cys Gly 
65                  70                  75                  80  


Thr Lys Phe Leu Ser Tyr Lys Phe Ser Asn Ser Gly Ser Arg Ile Thr 
                85                  90                  95      


Cys Ala Lys Gln Asp Ser Cys Arg Ser Gln Leu Cys Glu Cys Asp Lys 
            100                 105                 110         


Ala Ala Ala Thr Cys Phe Ala Arg Asn Lys Thr Thr Tyr Asn Lys Lys 
        115                 120                 125             


Tyr Gln Tyr Tyr Ser Asn Lys His Cys Arg Gly Ser Thr Pro Arg Cys 
    130                 135                 140                 


<210>  3
<211>  1911
<212>  DNA
<213>  Homo sapiens

<400>  3
cagggttcta cccggctggg tccaggcaga agtttttcct ccccacctcc gggtttgtcc       60

tcatcatcgg tcactcccat tcacagcttt aagattctgg aggccaagaa tttgactccc      120

cccggatcca tggtctgtgg ataccaatgt tccgactgga gacggggagc ccgcgagacc      180

cgggtctcca gggtctgccc aaggaagttg ctcatgggag cagacctcta gagcaggatt      240

tgaggccagg ccaaagagaa ccccagagat gaaaggcctc ctcccactgg cttggttcct      300

ggcttgtagt gtgcctgctg tgcaaggagg cttgctggac ctaaaatcaa tgatcgagaa      360

ggtgacaggg aagaacgccc tgacaaacta cggcttctac ggctgttact gcggctgggg      420

cggccgagga acccccaagg atggcaccga ttggtgctgt tgggcgcatg accactgcta      480

tgggcggctg gaggagaagg gctgcaacat tcgcacacag tcctacaaat acagattcgc      540

gtggggcgtg gtcacctgcg agcccgggcc cttctgccat gtgaacctct gtgcctgtga      600

ccggaagctc gtctactgcc tcaagagaaa cctacggagc tacaacccac agtaccaata      660

ctttcccaac atcctctgct cctaggcctc cccagcgagc tcctcccaga ccaagacttt      720

tgttctgttt ttctacaaca cagagtactg actctgcctg gttcctgaga gaggctccta      780

agtcacagac ctcagtcttt ctcgaagctt ggcggacccc cagggccaca ctgtaccctc      840

cagcgagtcc caggagagtg actctggtca taggacttgg tagggtccca gggtccctag      900

gcctccactt ctgagggcag cccctctggt gccaagagct ctcctccaac tcagggttgg      960

ctgtgtctct tttcttctct gaagacagcg tcctggctcc agttggaaca ctttcctgag     1020

atgcacttac ttctcagctt ctgcgatcag attatcatca ccaccaccct ccagagaatt     1080

tttacgcaag aagagccaaa ttgactctct aaatctggtg tatgggtatt aaataaaatt     1140

cattctcaag gctaataaaa accacattgg cattttcctc tgctgtgggg gatcgctggt     1200

gcctctttct ctgccactgg ggcaataaac ccaaagatgt ctacattatc tccgaaacag     1260

aagggaagat tagtaaatgc agggttttct gggatgagct tcaggctttc tcttgggcta     1320

attttcttac accttggggt cctctccagt attgggtctc attcttcctc gatggggtca     1380

gggaaagata actggtgatt atgccagctt cagcttccag gccagagagg gtggcattca     1440

aatcccagtg ctggcttctt cagctgtgtg gtcttggacc cgttactgaa cctctttgac     1500

tttcagtctc tttgagaaat aaactgtctt gttccttgca atgtaaaatg agacttctaa     1560

agcccacctt gatgctgata tggagaatgc tgaggttcta ggatttcaca cagcaggaat     1620

ttttttttaa taggtgtcag ctgtggggtt tattttttac aaagtaagga cattaaaaaa     1680

accaacccgt ctatcaattc ataaaagaaa ggatgttctg ataccaagac tgaaagaaga     1740

aaggatgtat tccaaaacaa aggaacatcc ttccaagaaa ggacctatgg cttctttatt     1800

ccgacatacc ccaaaataac tgcatgataa ataggtctat atttaaaaag ctctagtgtc     1860

gaatgttttc aaaataaaat ttaattttat gagaaaaaaa aaaaaaaaaa a              1911


<210>  4
<211>  138
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Lys Gly Leu Leu Pro Leu Ala Trp Phe Leu Ala Cys Ser Val Pro 
1               5                   10                  15      


Ala Val Gln Gly Gly Leu Leu Asp Leu Lys Ser Met Ile Glu Lys Val 
            20                  25                  30          


Thr Gly Lys Asn Ala Leu Thr Asn Tyr Gly Phe Tyr Gly Cys Tyr Cys 
        35                  40                  45              


Gly Trp Gly Gly Arg Gly Thr Pro Lys Asp Gly Thr Asp Trp Cys Cys 
    50                  55                  60                  


Trp Ala His Asp His Cys Tyr Gly Arg Leu Glu Glu Lys Gly Cys Asn 
65                  70                  75                  80  


Ile Arg Thr Gln Ser Tyr Lys Tyr Arg Phe Ala Trp Gly Val Val Thr 
                85                  90                  95      


Cys Glu Pro Gly Pro Phe Cys His Val Asn Leu Cys Ala Cys Asp Arg 
            100                 105                 110         


Lys Leu Val Tyr Cys Leu Lys Arg Asn Leu Arg Ser Tyr Asn Pro Gln 
        115                 120                 125             


Tyr Gln Tyr Phe Pro Asn Ile Leu Cys Ser 
    130                 135             


<210>  5
<211>  148
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Lys Leu Leu Val Leu Ala Val Leu Leu Thr Val Ala Ala Ala Asp 
1               5                   10                  15      


Ser Gly Ile Ser Pro Arg Ala Val Trp Gln Phe Arg Lys Met Ile Lys 
            20                  25                  30          


Cys Val Ile Pro Gly Ser Asp Pro Phe Leu Glu Tyr Asn Asn Tyr Gly 
        35                  40                  45              


Cys Tyr Cys Gly Leu Gly Gly Ser Gly Thr Pro Val Asp Glu Leu Asp 
    50                  55                  60                  


Lys Cys Cys Gln Thr His Asp Asn Cys Tyr Asp Gln Ala Lys Lys Leu 
65                  70                  75                  80  


Asp Ser Cys Lys Phe Leu Leu Asp Asn Pro Tyr Thr His Thr Tyr Ser 
                85                  90                  95      


Tyr Ser Cys Ser Gly Ser Ala Ile Thr Cys Ser Ser Lys Asn Lys Glu 
            100                 105                 110         


Cys Glu Ala Phe Ile Cys Asn Cys Asp Arg Asn Ala Ala Ile Cys Phe 
        115                 120                 125             


Ser Lys Ala Pro Tyr Asn Lys Ala His Lys Asn Leu Asp Thr Lys Lys 
    130                 135                 140                 


Tyr Cys Gln Ser 
145             


<210>  6
<211>  145
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Glu Leu Ala Leu Leu Cys Gly Leu Val Val Met Ala Gly Val Ile 
1               5                   10                  15      


Pro Ile Gln Gly Gly Ile Leu Asn Leu Asn Lys Met Val Lys Gln Val 
            20                  25                  30          


Thr Gly Lys Met Pro Ile Leu Ser Tyr Trp Pro Tyr Gly Cys His Cys 
        35                  40                  45              


Gly Leu Gly Gly Arg Gly Gln Pro Lys Asp Ala Thr Asp Trp Cys Cys 
    50                  55                  60                  


Gln Thr His Asp Cys Cys Tyr Asp His Leu Lys Thr Gln Gly Cys Ser 
65                  70                  75                  80  


Ile Tyr Lys Asp Tyr Tyr Arg Tyr Asn Phe Ser Gln Gly Asn Ile His 
                85                  90                  95      


Cys Ser Asp Lys Gly Ser Trp Cys Glu Gln Gln Leu Cys Ala Cys Asp 
            100                 105                 110         


Lys Glu Val Ala Phe Cys Leu Lys Arg Asn Leu Asp Thr Tyr Gln Lys 
        115                 120                 125             


Arg Leu Arg Phe Tyr Trp Arg Pro His Cys Arg Gly Gln Thr Pro Gly 
    130                 135                 140                 


Cys 
145 


<210>  7
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Lys Ser Pro His Val Leu Val Phe Leu Cys Leu Leu Val Ala Leu 
1               5                   10                  15      


Val Thr Gly Asn Leu Val Gln Phe Gly Val Met Ile Glu Lys Met Thr 
            20                  25                  30          


Gly Lys Ser Ala Leu Gln Tyr Asn Asp Tyr Gly Cys Tyr Cys Gly Ile 
        35                  40                  45              


Gly Gly Ser His Trp Pro Val Asp Gln Thr Asp Trp Cys Cys His Ala 
    50                  55                  60                  


His Asp Cys Cys Tyr Gly Arg Leu Glu Lys Leu Gly Cys Glu Pro Lys 
65                  70                  75                  80  


Leu Glu Lys Tyr Leu Phe Ser Val Ser Glu Arg Gly Ile Phe Cys Ala 
                85                  90                  95      


Gly Arg Thr Thr Cys Gln Arg Leu Thr Cys Glu Cys Asp Lys Arg Ala 
            100                 105                 110         


Ala Leu Cys Phe Arg Arg Asn Leu Gly Thr Tyr Asn Arg Lys Tyr Ala 
        115                 120                 125             


His Tyr Pro Asn Lys Leu Cys Thr Gly Pro Thr Pro Pro Cys 
    130                 135                 140         


<210>  8
<211>  168
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Lys Lys Phe Phe Thr Val Ala Ile Leu Ala Gly Ser Val Leu Ser 
1               5                   10                  15      


Thr Ala His Gly Ser Leu Leu Asn Leu Lys Ala Met Val Glu Ala Val 
            20                  25                  30          


Thr Gly Arg Ser Ala Ile Leu Ser Phe Val Gly Tyr Gly Cys Tyr Cys 
        35                  40                  45              


Gly Leu Gly Gly Arg Gly Gln Pro Lys Asp Glu Val Asp Trp Cys Cys 
    50                  55                  60                  


His Ala His Asp Cys Cys Tyr Gln Glu Leu Phe Asp Gln Gly Cys His 
65                  70                  75                  80  


Pro Tyr Val Asp His Tyr Asp His Thr Ile Glu Asn Asn Thr Glu Ile 
                85                  90                  95      


Val Cys Ser Asp Leu Asn Lys Thr Glu Cys Asp Lys Gln Thr Cys Met 
            100                 105                 110         


Cys Asp Lys Asn Met Val Leu Cys Leu Met Asn Gln Thr Tyr Arg Glu 
        115                 120                 125             


Glu Tyr Arg Gly Phe Leu Asn Val Tyr Cys Gln Gly Pro Thr Pro Asn 
    130                 135                 140                 


Cys Ser Ile Tyr Glu Pro Pro Pro Glu Glu Val Thr Cys Ser His Gln 
145                 150                 155                 160 


Ser Pro Ala Pro Pro Ala Pro Pro 
                165             


<210>  9
<211>  509
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Gly Val Gln Ala Gly Leu Phe Gly Met Leu Gly Phe Leu Gly Val 
1               5                   10                  15      


Ala Leu Gly Gly Ser Pro Ala Leu Arg Trp Tyr Arg Thr Ser Cys His 
            20                  25                  30          


Leu Thr Lys Ala Val Pro Gly Asn Pro Leu Gly Tyr Leu Ser Phe Leu 
        35                  40                  45              


Ala Lys Asp Ala Gln Gly Leu Ala Leu Ile His Ala Arg Trp Asp Ala 
    50                  55                  60                  


His Arg Arg Leu Gln Ser Cys Ser Trp Glu Asp Glu Pro Glu Leu Thr 
65                  70                  75                  80  


Ala Ala Tyr Gly Ala Leu Cys Ala His Glu Thr Ala Trp Gly Ser Phe 
                85                  90                  95      


Ile His Thr Pro Gly Pro Glu Leu Gln Arg Ala Leu Ala Thr Leu Gln 
            100                 105                 110         


Ser Gln Trp Glu Ala Cys Arg Ala Leu Glu Glu Ser Pro Ala Gly Ala 
        115                 120                 125             


Arg Lys Lys Arg Ala Ala Gly Gln Ser Gly Val Pro Gly Gly Gly His 
    130                 135                 140                 


Gln Arg Glu Lys Arg Gly Trp Thr Met Pro Gly Thr Leu Trp Cys Gly 
145                 150                 155                 160 


Val Gly Asp Ser Ala Gly Asn Ser Ser Glu Leu Gly Val Phe Gln Gly 
                165                 170                 175     


Pro Asp Leu Cys Cys Arg Glu His Asp Arg Cys Pro Gln Asn Ile Ser 
            180                 185                 190         


Pro Leu Gln Tyr Asn Tyr Gly Ile Arg Asn Tyr Arg Phe His Thr Ile 
        195                 200                 205             


Ser His Cys Asp Cys Asp Thr Arg Phe Gln Gln Cys Leu Gln Asn Gln 
    210                 215                 220                 


His Asp Ser Ile Ser Asp Ile Val Gly Val Ala Phe Phe Asn Val Leu 
225                 230                 235                 240 


Glu Ile Pro Cys Phe Val Leu Glu Glu Gln Glu Ala Cys Val Ala Trp 
                245                 250                 255     


Tyr Trp Trp Gly Gly Cys Arg Met Tyr Gly Thr Val Pro Leu Ala Arg 
            260                 265                 270         


Leu Gln Pro Arg Thr Phe Tyr Asn Ala Ser Trp Ser Ser Arg Ala Thr 
        275                 280                 285             


Ser Pro Thr Pro Ser Ser Arg Ser Pro Ala Pro Pro Lys Pro Arg Gln 
    290                 295                 300                 


Lys Gln His Leu Arg Lys Gly Pro Pro His Gln Lys Gly Ser Lys Arg 
305                 310                 315                 320 


Pro Ser Lys Ala Asn Thr Thr Ala Leu Gln Asp Pro Met Val Ser Pro 
                325                 330                 335     


Arg Leu Asp Val Ala Pro Thr Gly Leu Gln Gly Pro Gln Gly Gly Leu 
            340                 345                 350         


Lys Pro Gln Gly Ala Arg Trp Val Cys Arg Ser Phe Arg Arg His Leu 
        355                 360                 365             


Asp Gln Cys Glu His Gln Ile Gly Pro Arg Glu Ile Glu Phe Gln Leu 
    370                 375                 380                 


Leu Asn Ser Ala Gln Glu Pro Leu Phe His Cys Asn Cys Thr Arg Arg 
385                 390                 395                 400 


Leu Ala Arg Phe Leu Arg Leu His Ser Pro Pro Glu Val Thr Asn Met 
                405                 410                 415     


Leu Trp Glu Leu Leu Gly Thr Thr Cys Phe Lys Leu Ala Pro Pro Leu 
            420                 425                 430         


Asp Cys Val Glu Gly Lys Asn Cys Ser Arg Asp Pro Arg Ala Ile Arg 
        435                 440                 445             


Val Ser Ala Arg His Leu Arg Arg Leu Gln Gln Arg Arg His Gln Leu 
    450                 455                 460                 


Gln Asp Lys Gly Thr Asp Glu Arg Gln Pro Trp Pro Ser Glu Pro Leu 
465                 470                 475                 480 


Arg Gly Pro Met Ser Phe Tyr Asn Gln Cys Leu Gln Leu Thr Gln Ala 
                485                 490                 495     


Ala Arg Arg Pro Asp Arg Gln Gln Lys Ser Trp Ser Gln 
            500                 505                 


<210>  10
<211>  165
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Gly Pro Leu Pro Val Cys Leu Pro Ile Met Leu Leu Leu Leu Leu 
1               5                   10                  15      


Pro Ser Leu Leu Leu Leu Leu Leu Leu Pro Gly Pro Gly Ser Gly Glu 
            20                  25                  30          


Ala Ser Arg Ile Leu Arg Val His Arg Arg Gly Ile Leu Glu Leu Ala 
        35                  40                  45              


Gly Thr Val Gly Cys Val Gly Pro Arg Thr Pro Ile Ala Tyr Met Lys 
    50                  55                  60                  


Tyr Gly Cys Phe Cys Gly Leu Gly Gly His Gly Gln Pro Arg Asp Ala 
65                  70                  75                  80  


Ile Asp Trp Cys Cys His Gly His Asp Cys Cys Tyr Thr Arg Ala Glu 
                85                  90                  95      


Glu Ala Gly Cys Ser Pro Lys Thr Glu Arg Tyr Ser Trp Gln Cys Val 
            100                 105                 110         


Asn Gln Ser Val Leu Cys Gly Pro Ala Glu Asn Lys Cys Gln Glu Leu 
        115                 120                 125             


Leu Cys Lys Cys Asp Gln Glu Ile Ala Asn Cys Leu Ala Gln Thr Glu 
    130                 135                 140                 


Tyr Asn Leu Lys Tyr Leu Phe Tyr Pro Gln Phe Leu Cys Glu Pro Asp 
145                 150                 155                 160 


Ser Pro Lys Cys Asp 
                165 


<210>  11
<211>  189
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Ala Leu Leu Ser Arg Pro Ala Leu Thr Leu Leu Leu Leu Leu Met 
1               5                   10                  15      


Ala Ala Val Val Arg Cys Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg 
            20                  25                  30          


Ala Thr Leu Lys Thr Ile Arg Asn Gly Val His Lys Ile Asp Thr Tyr 
        35                  40                  45              


Leu Asn Ala Ala Leu Asp Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln 
    50                  55                  60                  


Tyr Lys Cys Ser Asp Gly Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys 
65                  70                  75                  80  


Pro Ser Pro Pro Asn Gly Cys Gly Ser Pro Leu Phe Gly Val His Leu 
                85                  90                  95      


Asn Ile Gly Ile Pro Ser Leu Thr Lys Cys Cys Asn Gln His Asp Arg 
            100                 105                 110         


Cys Tyr Glu Thr Cys Gly Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe 
        115                 120                 125             


Gln Tyr Cys Leu Ser Lys Ile Cys Arg Asp Val Gln Lys Thr Leu Gly 
    130                 135                 140                 


Leu Thr Gln His Val Gln Ala Cys Glu Thr Thr Val Glu Leu Leu Phe 
145                 150                 155                 160 


Asp Ser Val Ile His Leu Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg 
                165                 170                 175     


Ala Ala Cys Arg Cys His Tyr Glu Glu Lys Thr Asp Leu 
            180                 185                 


<210>  12
<211>  195
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Lys Leu Ala Ser Gly Phe Leu Val Leu Trp Leu Ser Leu Gly Gly 
1               5                   10                  15      


Gly Leu Ala Gln Ser Asp Thr Ser Pro Asp Thr Glu Glu Ser Tyr Ser 
            20                  25                  30          


Asp Trp Gly Leu Arg His Leu Arg Gly Ser Phe Glu Ser Val Asn Ser 
        35                  40                  45              


Tyr Phe Asp Ser Phe Leu Glu Leu Leu Gly Gly Lys Asn Gly Val Cys 
    50                  55                  60                  


Gln Tyr Arg Cys Arg Tyr Gly Lys Ala Pro Met Pro Arg Pro Gly Tyr 
65                  70                  75                  80  


Lys Pro Gln Glu Pro Asn Gly Cys Gly Ser Tyr Phe Leu Gly Leu Lys 
                85                  90                  95      


Val Pro Glu Ser Met Asp Leu Gly Ile Pro Ala Met Thr Lys Cys Cys 
            100                 105                 110         


Asn Gln Leu Asp Val Cys Tyr Asp Thr Cys Gly Ala Asn Lys Tyr Arg 
        115                 120                 125             


Cys Asp Ala Lys Phe Arg Trp Cys Leu His Ser Ile Cys Ser Asp Leu 
    130                 135                 140                 


Lys Arg Ser Leu Gly Phe Val Ser Lys Val Glu Ala Ala Cys Asp Ser 
145                 150                 155                 160 


Leu Val Asp Thr Val Phe Asn Thr Val Trp Thr Leu Gly Cys Arg Pro 
                165                 170                 175     


Phe Met Asn Ser Gln Arg Ala Ala Cys Ile Cys Ala Glu Glu Glu Lys 
            180                 185                 190         


Glu Glu Leu 
        195 


