                         SEQUENCE LISTING

<110>  The Henry M. Jackson Foundation For The Advancement of 
       Military Medicine, Inc.
 
<120>  METHODS AND COMPOSITIONS BASED ON SHIGA TOXIN TYPE 2 PROTEIN

<130>  50111/117WO3

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1

Asn His Thr Pro Pro Gly Ser Tyr 
1               5               


<210>  2
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Thr His Ile Ser Val 
1               5   


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Gln Gly Ala Arg Ser Val Arg Ala Val Asn Glu Glu Ser Gln Pro Glu 
1               5                   10                  15      


<210>  4
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asn 
    50                  55                  60                  


His Thr Pro Pro Gly Ser Tyr Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Ser His Val Thr Phe Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His His Ala Ser Arg Val Ala 
            260                 265                 270         


Arg Met Ala Ser Asp Glu Phe Pro Ser Met Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  5
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asn 
    50                  55                  60                  


His Thr Pro Pro Gly Ser Tyr Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Thr His Ile Ser Val Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His His Ala Ser Arg Val Ala 
            260                 265                 270         


Arg Met Ala Ser Asp Glu Phe Pro Ser Met Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  6
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asn 
    50                  55                  60                  


His Thr Pro Pro Gly Ser Tyr Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Ser His Val Thr Phe Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His Gln Gly Ala Arg Ser Val 
            260                 265                 270         


Arg Ala Val Asn Glu Glu Ser Gln Pro Glu Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  7
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asp 
    50                  55                  60                  


Ser Gly Ser Gly Asp Asn Leu Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Thr His Ile Ser Val Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His Gln Gly Ala Arg Ser Val 
            260                 265                 270         


Arg Ala Val Asn Glu Glu Ser Gln Pro Glu Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  8
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asn 
    50                  55                  60                  


His Thr Pro Pro Gly Ser Tyr Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Thr His Ile Ser Val Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His Gln Gly Ala Arg Ser Val 
            260                 265                 270         


Arg Ala Val Asn Glu Glu Ser Gln Pro Glu Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  9
<211>  1227
<212>  DNA
<213>  Escherichia coli

<400>  9
atgaaaataa ttatttttag agtgctaact tttttctttg ttatcttttc agttaatgtg       60

gtggcgaagg aatttacctt agacttctcg actgcaaaga cgtatgtaga ttcgctgaat      120

gtcattcgct ctgcaatagg tactccatta cagactattt catcaggagg tacgtcttta      180

ctgatgattg atagtggctc aggggataat ttgtttgcag ttgatgtcag agggatagat      240

ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa tttatatgtg      300

acaggatttg ttaacaggac aaataatgtt ttttatcgct ttgctgattt ttcacatgtt      360

acctttccag gtacaacagc ggttacattg tctggtgaca gtagctatac cacgttacag      420

cgtgttgcag ggatcagtcg tacggggatg cagataaatc gccattcgtt gactacttct      480

tatctggatt taatgtcgca tagtggaacc tcactgacgc agtctgtggc aagagcgatg      540

ttacggtttg ttactgtgac agctgaagct ttacgttttc ggcaaataca gaggggattt      600

cgtacaacac tggatgatct cagtgggcgt tcttatgtaa tgactgctga agatgttgat      660

cttacattga actggggaag gttgagtagc gtcctgcctg actatcatgg acaagactct      720

gttcgtgtag gaagaatttc ttttggaagc attaatgcaa ttctgggaag cgtggcatta      780

atactgaatt gtcatcatca tgcatcgcga gttgccagaa tggcatctga tgagtttcct      840

tctatgtgtc cggcagatgg aagagtccgt gggattacgc acaataaaat attgtgggat      900

tcatccactc tgggggcaat tctgatgcgc agaactatta gcagttgagg gggtaaaatg      960

aaaaaaacat tattaatagc tgcatcgctt tcattttttt cagcaagtgc gctggcgacg     1020

cctgattgtg taactggaaa ggtggagtat acaaaatata atgatgacga tacctttaca     1080

gttaaagtgg gtgataaaga attatttacc aacagatgga atcttcagtc tcttcttctc     1140

agtgcgcaaa ttacggggat gactgtaacc attaaaacta atgcctgtca taatggaggg     1200

ggattcagcg aagttatttt tcgttga                                         1227


<210>  10
<211>  948
<212>  DNA
<213>  Escherichia coli

<400>  10
atgaaaataa ttatttttag agtgctaact tttttctttg ttatcttttc agttaatgtg       60

gtggcgaagg aatttacctt agacttctcg actgcaaaga cgtatgtaga ttcgctgaat      120

gtcattcgct ctgcaatagg tactccatta cagactattt catcaggagg tacgtcttta      180

ctgatgattg atagtggctc aggggataat ttgtttgcag ttgatgtcag agggatagat      240

ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa tttatatgtg      300

acaggatttg ttaacaggac aaataatgtt ttttatcgct ttgctgattt ttcacatgtt      360

acctttccag gtacaacagc ggttacattg tctggtgaca gtagctatac cacgttacag      420

cgtgttgcag ggatcagtcg tacggggatg cagataaatc gccattcgtt gactacttct      480

tatctggatt taatgtcgca tagtggaacc tcactgacgc agtctgtggc aagagcgatg      540

ttacggtttg ttactgtgac agctgaagct ttacgttttc ggcaaataca gaggggattt      600

cgtacaacac tggatgatct cagtgggcgt tcttatgtaa tgactgctga agatgttgat      660

cttacattga actggggaag gttgagtagc gtcctgcctg actatcatgg acaagactct      720

gttcgtgtag gaagaatttc ttttggaagc attaatgcaa ttctgggaag cgtggcatta      780

atactgaatt gtcatcatca tgcatcgcga gttgccagaa tggcatctga tgagtttcct      840

tctatgtgtc cggcagatgg aagagtccgt gggattacgc acaataaaat attgtgggat      900

tcatccactc tgggggcaat tctgatgcgc agaactatta gcagttga                   948


<210>  11
<211>  270
<212>  DNA
<213>  Escherichia coli

<400>  11
atgaaaaaaa cattattaat agctgcatcg ctttcatttt tttcagcaag tgcgctggcg       60

acgcctgatt gtgtaactgg aaaggtggag tatacaaaat ataatgatga cgataccttt      120

acagttaaag tgggtgataa agaattattt accaacagat ggaatcttca gtctcttctt      180

ctcagtgcgc aaattacggg gatgactgta accattaaaa ctaatgcctg tcataatgga      240

gggggattca gcgaagttat ttttcgttga                                       270


<210>  12
<211>  315
<212>  PRT
<213>  Escherichia coli

<400>  12

Met Lys Ile Ile Ile Phe Arg Val Leu Thr Phe Phe Phe Val Ile Phe 
1               5                   10                  15      


Ser Val Asn Val Val Ala Lys Glu Phe Thr Leu Asp Phe Ser Thr Ala 
            20                  25                  30          


Lys Thr Tyr Val Asp Ser Leu Asn Val Ile Arg Ser Ala Ile Gly Thr 
        35                  40                  45              


Pro Leu Gln Thr Ile Ser Ser Gly Gly Thr Ser Leu Leu Met Ile Asp 
    50                  55                  60                  


Ser Gly Ser Gly Asp Asn Leu Phe Ala Val Asp Val Arg Gly Ile Asp 
65                  70                  75                  80  


Pro Glu Glu Gly Arg Phe Asn Asn Leu Arg Leu Ile Val Glu Arg Asn 
                85                  90                  95      


Asn Leu Tyr Val Thr Gly Phe Val Asn Arg Thr Asn Asn Val Phe Tyr 
            100                 105                 110         


Arg Phe Ala Asp Phe Ser His Val Thr Phe Pro Gly Thr Thr Ala Val 
        115                 120                 125             


Thr Leu Ser Gly Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Gly 
    130                 135                 140                 


Ile Ser Arg Thr Gly Met Gln Ile Asn Arg His Ser Leu Thr Thr Ser 
145                 150                 155                 160 


Tyr Leu Asp Leu Met Ser His Ser Gly Thr Ser Leu Thr Gln Ser Val 
                165                 170                 175     


Ala Arg Ala Met Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Gly Phe Arg Thr Thr Leu Asp Asp Leu Ser 
        195                 200                 205             


Gly Arg Ser Tyr Val Met Thr Ala Glu Asp Val Asp Leu Thr Leu Asn 
    210                 215                 220                 


Trp Gly Arg Leu Ser Ser Val Leu Pro Asp Tyr His Gly Gln Asp Ser 
225                 230                 235                 240 


Val Arg Val Gly Arg Ile Ser Phe Gly Ser Ile Asn Ala Ile Leu Gly 
                245                 250                 255     


Ser Val Ala Leu Ile Leu Asn Cys His His His Ala Ser Arg Val Ala 
            260                 265                 270         


Arg Met Ala Ser Asp Glu Phe Pro Ser Met Cys Pro Ala Asp Gly Arg 
        275                 280                 285             


Val Arg Gly Ile Thr His Asn Lys Ile Leu Trp Asp Ser Ser Thr Leu 
    290                 295                 300                 


Gly Ala Ile Leu Met Arg Arg Thr Ile Ser Ser 
305                 310                 315 


<210>  13
<211>  89
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Lys Lys Thr Leu Leu Ile Ala Ala Ser Leu Ser Phe Phe Ser Ala 
1               5                   10                  15      


Ser Ala Leu Ala Thr Pro Asp Cys Val Thr Gly Lys Val Glu Tyr Thr 
            20                  25                  30          


Lys Tyr Asn Asp Asp Asp Thr Phe Thr Val Lys Val Gly Asp Lys Glu 
        35                  40                  45              


Leu Phe Thr Asn Arg Trp Asn Leu Gln Ser Leu Leu Leu Ser Ala Gln 
    50                  55                  60                  


Ile Thr Gly Met Thr Val Thr Ile Lys Thr Asn Ala Cys His Asn Gly 
65                  70                  75                  80  


Gly Gly Phe Ser Glu Val Ile Phe Arg 
                85                  


<210>  14
<211>  1241
<212>  DNA
<213>  Escherichia coli

<400>  14
atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc       60

tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat      120

agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg      180

tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat      240

gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg      300

gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata      360

tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa      420

cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca      480

tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt      540

ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt      600

cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc      660

actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc      720

agagtgggga gaatatcctt taataatata tcagcgatac tggggactgt ggccgttata      780

ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca      840

gaatgtcaga taactggcga caggcctgtt ataaaaataa acaatacatt atgggaaagt      900

aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa      960

aggagttaag catgaagaag atgtttatgg cggttttatt tgcattagct tctgttaatg     1020

caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat gaggatgaca     1080

catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat ctgcaaccgt     1140

tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat     1200

caggctccgg atttgctgaa gtgcagttta ataatgactg a                         1241


<210>  15
<211>  960
<212>  DNA
<213>  Escherichia coli

<400>  15
atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc       60

tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat      120

agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg      180

tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat      240

gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg      300

gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata      360

tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa      420

cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca      480

tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt      540

ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt      600

cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc      660

actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc      720

agagtgggga gaatatcctt taataatata tcagcgatac tggggactgt ggccgttata      780

ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca      840

gaatgtcaga taactggcga caggcctgtt ataaaaataa acaatacatt atgggaaagt      900

aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa      960


<210>  16
<211>  270
<212>  DNA
<213>  Escherichia coli

<400>  16
atgaagaaga tgtttatggc ggttttattt gcattagctt ctgttaatgc aatggcggcg       60

gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac atttacagtg      120

aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt actgcaaagt      180

gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc aggctccgga      240

tttgctgaag tgcagtttaa taatgactga                                       270


<210>  17
<211>  319
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Lys Cys Ile Leu Phe Lys Trp Val Leu Cys Leu Leu Leu Gly Phe 
1               5                   10                  15      


Ser Ser Val Ser Tyr Ser Arg Glu Phe Thr Ile Asp Phe Ser Thr Gln 
            20                  25                  30          


Gln Ser Tyr Val Ser Ser Leu Asn Ser Ile Arg Thr Glu Ile Ser Thr 
        35                  40                  45              


Pro Leu Glu His Ile Ser Gln Gly Thr Thr Ser Val Ser Val Ile Asn 
    50                  55                  60                  


His Thr Pro Pro Gly Ser Tyr Phe Ala Val Asp Ile Arg Gly Leu Asp 
65                  70                  75                  80  


Val Tyr Gln Ala Arg Phe Asp His Leu Arg Leu Ile Ile Glu Gln Asn 
                85                  90                  95      


Asn Leu Tyr Val Ala Gly Phe Val Asn Thr Ala Thr Asn Thr Phe Tyr 
            100                 105                 110         


Arg Phe Ser Asp Phe Thr His Ile Ser Val Pro Gly Val Thr Thr Val 
        115                 120                 125             


Ser Met Thr Thr Asp Ser Ser Tyr Thr Thr Leu Gln Arg Val Ala Ala 
    130                 135                 140                 


Leu Glu Arg Ser Gly Met Gln Ile Ser Arg His Ser Leu Val Ser Ser 
145                 150                 155                 160 


Tyr Leu Ala Leu Met Glu Phe Ser Gly Asn Thr Met Thr Arg Asp Ala 
                165                 170                 175     


Ser Arg Ala Val Leu Arg Phe Val Thr Val Thr Ala Glu Ala Leu Arg 
            180                 185                 190         


Phe Arg Gln Ile Gln Arg Glu Phe Arg Gln Ala Leu Ser Glu Thr Ala 
        195                 200                 205             


Pro Val Tyr Thr Met Thr Pro Gly Asp Val Asp Leu Thr Leu Asn Trp 
    210                 215                 220                 


Gly Arg Ile Ser Asn Val Leu Pro Glu Tyr Arg Gly Glu Asp Gly Val 
225                 230                 235                 240 


Arg Val Gly Arg Ile Ser Phe Asn Asn Ile Ser Ala Ile Leu Gly Thr 
                245                 250                 255     


Val Ala Val Ile Leu Asn Cys His His Gln Gly Ala Arg Ser Val Arg 
            260                 265                 270         


Ala Val Asn Glu Glu Ser Gln Pro Glu Cys Gln Ile Thr Gly Asp Arg 
        275                 280                 285             


Pro Val Ile Lys Ile Asn Asn Thr Leu Trp Glu Ser Asn Thr Ala Ala 
    290                 295                 300                 


Ala Phe Leu Asn Arg Lys Ser Gln Phe Leu Tyr Thr Thr Gly Lys 
305                 310                 315                 


<210>  18
<211>  89
<212>  PRT
<213>  Escherichia coli

<400>  18

Met Lys Lys Met Phe Met Ala Val Leu Phe Ala Leu Ala Ser Val Asn 
1               5                   10                  15      


Ala Met Ala Ala Asp Cys Ala Lys Gly Lys Ile Glu Phe Ser Lys Tyr 
            20                  25                  30          


Asn Glu Asp Asp Thr Phe Thr Val Lys Val Asp Gly Lys Glu Tyr Trp 
        35                  40                  45              


Thr Ser Arg Trp Asn Leu Gln Pro Leu Leu Gln Ser Ala Gln Leu Thr 
    50                  55                  60                  


Gly Met Thr Val Thr Ile Lys Ser Ser Thr Cys Glu Ser Gly Ser Gly 
65                  70                  75                  80  


Phe Ala Glu Val Gln Phe Asn Asn Asp 
                85                  


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19

Ala His Ile Ser Leu 
1               5   


<210>  20
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20
taaggaggac agctatg                                                      17


<210>  21
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21
gatcggatcc ccctgtaacg aagtttgcgt aacagc                                 36


<210>  22
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  22
gatcgaattc tcgcttacga tcatcaaaga gatcatacc                              39


<210>  23
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23
gatcggatcc agcaagggcc accatatcac ataccgcc                               38


<210>  24
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24
caggggaatt caccatgcga aattttttta acaaatgc                               38


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  25
gaacatatat ctcaggggac cac                                               23


<210>  26
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  26
gtggtcccct gagatatatg ttctaatgga gtacctattg cagagcg                     47


<210>  27
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27
ttacggtttg ttactgtgac agctgaagc                                         29


<210>  28
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  28
gcttcagctg tcacagtaac aaaccgtaaa actgctctgg atgcatctct ggt              53


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  29
cagataaatc gccattcgtt ga                                                22


<210>  30
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  30
tcaacgaatg gcgatttatc tgcattccgg aacgttccag cgc                         43


<210>  31
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  31
ggtacgtctt tactgatgat taaccacacc ccaccgggca gttattttgc                  50


<210>  32
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  32
gcaaaataac tgcccggtgg ggtgtggtta atcatcagta aagacgtacc                  50


<210>  33
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  33
tatgtgacag gatttgttaa caggac                                            26


<210>  34
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  34
gtcctgttaa caaatcctgt cacatataaa ttattttgct caataatcag acgaagatgg       60


<210>  35
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  35
aggaggacag ctatgaaaat aattattttt agagtgcta                              39


<210>  36
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36
gatcggatcc taaggaggac agctatgaaa ataatt                                 36


<210>  37
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  37
ggtggtggtg acgaaaaata acttcgctga atcc                                   34


<210>  38
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  38
cagtggtggt ggtggtggtg acgaaaaata ac                                     32


<210>  39
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  39
gatcgaattc tcagtggtgg tggtggtggt g                                      31


<210>  40
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  40
aaccacaccc caccgggcag ttattttgca gttgatgtca gaggg                       45


<210>  41
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  41
ataactgccc ggtggggtgt ggttaatcat cagtaaagac gtacc                       45


<210>  42
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  42
acacatatat cagtgccagg tacaacagcg gttacattgt ctgg                        44


<210>  43
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  43
acctggcact gatatatgtg taaaatcagc aaagcgataa aaaaca                      46


<210>  44
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  44
gtgaatgaag agagtcaacc agaatgtccg gcagatggaa gagtccg                     47


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  45
ttctggttga ctctcttcat tcac                                              24


<210>  46
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  46
ggcattaata ctgaattgtc atcatcaggg ggcgcgttct gttcgc                      46


<210>  47
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  47
atgatgacaa ttcagtatta atgcc                                             25


<210>  48
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  48
aggaggacag ctatgaagtg tatattattt aaatgggt                               38


<210>  49
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  49
gatcggatcc taaggaggac agctatgaag tgta                                   34


<210>  50
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  50
ggtggtggtg gtcattatta aactgcactt c                                      31


<210>  51
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  51
cagtggtggt ggtggtggtg gtcattatta aa                                     32


<210>  52
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  52
gatcggatcc ctggtatcgt attacttcag cc                                     32


<210>  53
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  53
gatcgaattc ctgcacacta cgaaaccagc                                        30


<210>  54
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  54
tcagtgacag gatttgttaa caggac                                            26


<210>  55
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  55
gtcctgttaa caaatcctgt cactgataaa ttatttcgtt caacaataag ccg              53


