                         SEQUENCE LISTING

<110>  Luminos, LLC
 
<120>  METHODS AND COMPOSITIONS RELATING TO ENZYME ASSAYS

<130>  LUM-11052/47

<150>  12/353,723
<151>  2009-01-14

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  substrate for human JmjD2a


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  METHYLATION, tri-methyl

<400>  1

Ala Arg Thr Lys Gln Thr Ala Arg Lys Ser Thr Gly Gly Lys Ala 
1               5                   10                  15  


<210>  2
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  substrate for histone lysine demethylases


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  METHYLATION, dimethyl

<400>  2

Ala Arg Thr Lys Gln Thr Ala Arg Lys Ser Thr Gly Gly Lys Ala Pro 
1               5                   10                  15      


Arg Lys Gln Leu Ala 
            20      


<210>  3
<211>  350
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Ser Glu Ser Glu Thr Leu Asn Pro Ser Ala Arg Ile Met Thr 
1               5                   10                  15      


Phe Tyr Pro Thr Met Glu Glu Phe Arg Asn Phe Ser Arg Tyr Ile Ala 
            20                  25                  30          


Tyr Ile Glu Ser Gln Gly Ala His Arg Ala Gly Leu Ala Lys Val Val 
        35                  40                  45              


Pro Pro Lys Glu Trp Lys Pro Arg Ala Ser Tyr Asp Asp Ile Asp Asp 
    50                  55                  60                  


Leu Val Ile Pro Ala Pro Ile Gln Gln Leu Val Thr Gly Gln Ser Gly 
65                  70                  75                  80  


Leu Phe Thr Gln Tyr Asn Ile Gln Lys Lys Ala Met Thr Val Arg Glu 
                85                  90                  95      


Phe Arg Lys Ile Ala Asn Ser Asp Lys Tyr Cys Thr Pro Arg Tyr Ser 
            100                 105                 110         


Glu Phe Glu Glu Leu Glu Arg Lys Tyr Trp Lys Asn Leu Thr Phe Asn 
        115                 120                 125             


Pro Pro Ile Tyr Gly Ala Asp Val Asn Gly Thr Leu Tyr Glu Lys His 
    130                 135                 140                 


Val Asp Glu Trp Asn Ile Gly Arg Leu Arg Thr Ile Leu Asp Leu Val 
145                 150                 155                 160 


Glu Lys Glu Ser Gly Ile Thr Ile Glu Gly Val Asn Thr Pro Tyr Leu 
                165                 170                 175     


Tyr Phe Gly Met Trp Lys Thr Ser Phe Ala Trp His Thr Glu Asp Met 
            180                 185                 190         


Asp Leu Tyr Ser Ile Asn Tyr Leu His Phe Gly Glu Pro Lys Ser Trp 
        195                 200                 205             


Tyr Ser Val Pro Pro Glu His Gly Lys Arg Leu Glu Arg Leu Ala Lys 
    210                 215                 220                 


Gly Phe Phe Pro Gly Ser Ala Gln Ser Cys Glu Ala Phe Leu Arg His 
225                 230                 235                 240 


Lys Met Thr Leu Ile Ser Pro Leu Met Leu Lys Lys Tyr Gly Ile Pro 
                245                 250                 255     


Phe Asp Lys Val Thr Gln Glu Ala Gly Glu Phe Met Ile Thr Phe Pro 
            260                 265                 270         


Tyr Gly Tyr His Ala Gly Phe Asn His Gly Phe Asn Cys Ala Glu Ser 
        275                 280                 285             


Thr Asn Phe Ala Thr Arg Arg Trp Ile Glu Tyr Gly Lys Gln Ala Val 
    290                 295                 300                 


Leu Cys Ser Cys Arg Lys Asp Met Val Lys Ile Ser Met Asp Val Phe 
305                 310                 315                 320 


Val Arg Lys Phe Gln Pro Glu Arg Tyr Lys Leu Trp Lys Ala Gly Lys 
                325                 330                 335     


Asp Asn Thr Val Ile Asp His Thr Leu Pro Thr Pro Glu Ala 
            340                 345                 350 


<210>  4
<211>  702
<212>  PRT
<213>  Homo sapiens

<400>  4

Leu Pro Pro Pro Pro Pro Gln Ala Pro Pro Glu Glu Glu Asn Glu Ser 
1               5                   10                  15      


Glu Pro Glu Glu Pro Ser Gly Val Glu Gly Ala Ala Phe Gln Ser Arg 
            20                  25                  30          


Leu Pro His Asp Arg Met Thr Ser Gln Glu Ala Ala Cys Phe Pro Asp 
        35                  40                  45              


Ile Ile Ser Gly Pro Gln Gln Thr Gln Lys Val Phe Leu Phe Ile Arg 
    50                  55                  60                  


Asn Arg Thr Leu Gln Leu Trp Leu Asp Asn Pro Lys Ile Gln Leu Thr 
65                  70                  75                  80  


Phe Glu Ala Thr Leu Gln Gln Leu Glu Ala Pro Tyr Asn Ser Asp Thr 
                85                  90                  95      


Val Leu Val His Arg Val His Ser Tyr Leu Glu Arg His Gly Leu Ile 
            100                 105                 110         


Asn Phe Gly Ile Tyr Lys Arg Ile Lys Pro Leu Pro Thr Lys Lys Thr 
        115                 120                 125             


Gly Lys Val Ile Ile Ile Gly Ser Gly Val Ser Gly Leu Ala Ala Ala 
    130                 135                 140                 


Arg Gln Leu Gln Ser Phe Gly Met Asp Val Thr Leu Leu Glu Ala Arg 
145                 150                 155                 160 


Asp Arg Val Gly Gly Arg Val Ala Thr Phe Arg Lys Gly Asn Tyr Val 
                165                 170                 175     


Ala Asp Leu Gly Ala Met Val Val Thr Gly Leu Gly Gly Asn Pro Met 
            180                 185                 190         


Ala Val Val Ser Lys Gln Val Asn Met Glu Leu Ala Lys Ile Lys Gln 
        195                 200                 205             


Lys Cys Pro Leu Tyr Glu Ala Asn Gly Gln Ala Val Pro Lys Glu Lys 
    210                 215                 220                 


Asp Glu Met Val Glu Gln Glu Phe Asn Arg Leu Leu Glu Ala Thr Ser 
225                 230                 235                 240 


Tyr Leu Ser His Gln Leu Asp Phe Asn Val Leu Asn Asn Lys Pro Val 
                245                 250                 255     


Ser Leu Gly Gln Ala Leu Glu Val Val Ile Gln Leu Gln Glu Lys His 
            260                 265                 270         


Val Lys Asp Glu Gln Ile Glu His Trp Lys Lys Ile Val Lys Thr Gln 
        275                 280                 285             


Glu Glu Leu Lys Glu Leu Leu Asn Lys Met Val Asn Leu Lys Glu Lys 
    290                 295                 300                 


Ile Lys Glu Leu His Gln Gln Tyr Lys Glu Ala Ser Glu Val Lys Pro 
305                 310                 315                 320 


Pro Arg Asp Ile Thr Ala Glu Phe Leu Val Lys Ser Lys His Arg Asp 
                325                 330                 335     


Leu Thr Ala Leu Cys Lys Glu Tyr Asp Glu Leu Ala Glu Thr Gln Gly 
            340                 345                 350         


Lys Leu Glu Glu Lys Leu Gln Glu Leu Glu Ala Asn Pro Pro Ser Asp 
        355                 360                 365             


Val Tyr Leu Ser Ser Arg Asp Arg Gln Ile Leu Asp Trp His Phe Ala 
    370                 375                 380                 


Asn Leu Glu Phe Ala Asn Ala Thr Pro Leu Ser Thr Leu Ser Leu Lys 
385                 390                 395                 400 


His Trp Asp Gln Asp Asp Asp Phe Glu Phe Thr Gly Ser His Leu Thr 
                405                 410                 415     


Val Arg Asn Gly Tyr Ser Cys Val Pro Val Ala Leu Ala Glu Gly Leu 
            420                 425                 430         


Asp Ile Lys Leu Asn Thr Ala Val Arg Gln Val Arg Tyr Thr Ala Ser 
        435                 440                 445             


Gly Cys Glu Val Ile Ala Val Asn Thr Arg Ser Thr Ser Gln Thr Phe 
    450                 455                 460                 


Ile Tyr Lys Cys Asp Ala Val Leu Cys Thr Leu Pro Leu Gly Val Leu 
465                 470                 475                 480 


Lys Gln Gln Pro Pro Ala Val Gln Phe Val Pro Pro Leu Pro Glu Trp 
                485                 490                 495     


Lys Thr Ser Ala Val Gln Arg Met Gly Phe Gly Asn Leu Asn Lys Val 
            500                 505                 510         


Val Leu Cys Phe Asp Arg Val Phe Trp Asp Pro Ser Val Asn Leu Phe 
        515                 520                 525             


Gly His Val Gly Ser Thr Thr Ala Ser Arg Gly Glu Leu Phe Leu Phe 
    530                 535                 540                 


Trp Asn Leu Tyr Lys Ala Pro Ile Leu Leu Ala Leu Val Ala Gly Glu 
545                 550                 555                 560 


Ala Ala Gly Ile Met Glu Asn Ile Ser Asp Asp Val Ile Val Gly Arg 
                565                 570                 575     


Cys Leu Ala Ile Leu Lys Gly Ile Phe Gly Ser Ser Ala Val Pro Gln 
            580                 585                 590         


Pro Lys Glu Thr Val Val Ser Arg Trp Arg Ala Asp Pro Trp Ala Arg 
        595                 600                 605             


Gly Ser Tyr Ser Tyr Val Ala Ala Gly Ser Ser Gly Asn Asp Tyr Asp 
    610                 615                 620                 


Leu Met Ala Gln Pro Ile Thr Pro Gly Pro Ser Ile Pro Gly Ala Pro 
625                 630                 635                 640 


Gln Pro Ile Pro Arg Leu Phe Phe Ala Gly Glu His Thr Ile Arg Asn 
                645                 650                 655     


Tyr Pro Ala Thr Val His Gly Ala Leu Leu Ser Gly Leu Arg Glu Ala 
            660                 665                 670         


Gly Arg Ile Ala Asp Gln Phe Leu Gly Ala Met Tyr Thr Leu Pro Arg 
        675                 680                 685             


Gln Ala Thr Pro Gly Val Pro Ala Gln Gln Ser Pro Ser Met 
    690                 695                 700         


