                         SEQUENCE LISTING

<110>  Syracuse University
 
<120>  SYSTEM AND METHOD FOR THE HETEROLOGOUS EXPRESSION OF POLYKETIDE 
       SYNTHASE GENE CLUSTERS

<130>  156P156

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  1517
<212>  DNA
<213>  Myxococcus xanthus

<400>  1
atggcgatgg aactgaaaca aagcctgaag cttgcccaac agctggtgat gacgccgcag       60

ctgcagcaag ccatcaagct cctccaactg tctcgcatgg aactgctcga gcaggtccgc      120

gaggagatgg agcagaaccc gctgctggag caaccagacg agcaagcgcc gggggatgtg      180

ggagacaagg aacccgggga agcctccctg gaagcagaca acatggaggt gccgcgggac      240

gtggatctgc ccgcggccac cagcgacacc gccacggagt tcaaggcgga cggggagggc      300

cccccggaaa tcgactggga gcagtacctc aacagctacc agttcaatga gcccaccacg      360

gcctccaaca agggcaacgt ggccacggac gacatgccgt cgttcgaggc caacctcgtc      420

aagaaggcgg acctggtcga ccacatccag gagcagctgg gcacgctgcg cctgaatgac      480

gccgagcgcc gcatcgccat gctcatcctg ggcaacctgg atgacgacgg ctacctcaag      540

ctgccggaag tggacgggga tccgctcatc cgcctggcca acgaggcgga cgtgcccatg      600

cacgtcgcgg agcgcacgct gcggcgcatc cagatgttgg atccgcgcgg ctgcggcgcc      660

cgtgacttgc aggagtgcct gctcatccag ctccagggca tcagggagcc gcacgcgccg      720

ctgttgggcc tcatcatcaa gcggcacatg aagtacctgg agagcaagaa cctgcccgcc      780

atcgccaagg acctgaaggt caccttggaa gaggtggtgg gggcggtgcg gctgctcccg      840

aagctggacc cgaagccggg ccgcaacttc agcggggacg acgcgcagta catctccccc      900

gacgtgttcg tctacaagat gggggacgac tacacggtgg tgctcaacga tgacggcctg      960

tccaagctgc gcatctccgg cacctaccgg aacgcgctga agacgggcgc ggtgggcccg     1020

ggccagacga aggacttcat ccaggacaag ctgcgcagcg cgatgtggct catccgctcc     1080

atccaccagc ggcagcggac catctacaaa gtcaccgaag catcgtgaag ttccagcggg     1140

acttcctgga caagggcatt gcctacctca agccgctcat cctccgggac gtggccgagg     1200

acatcggcat gcacgagtcc acggtgagcc gcgtcaccac cagcaagtac gtgcacacac     1260

cgcagggcat cttcgagctg aagtacttct tcaactcgtc catcgcccgc gtctccggtg     1320

aggacaccgc gagcgaggcg gtgaagcacc acatcaagca gttggtggcg caggaagacg     1380

cccgcaaccc gtactcggac cagaaaatcg tcgagctgct gcgctcgcag ggcaccgaga     1440

ttgcccgccg cacggtggcc aagtaccgcg aggtgctggg catcctcccc agcagcaagc     1500

gcaagcggta ctactga                                                    1517


<210>  2
<211>  1434
<212>  DNA
<213>  Escherichia coli

<400>  2
atgaagcaag gtttgcaact caggcttagc caacaactgg cgatgacgcc acagctccaa       60

caggcaattc gtctgttgca gttgtcgacg ctggaacttc agcaggagct acagcaggcg      120

ctggagagta atccgctgct tgagcaaatc gacactcatg aagaaatcga cacccgcgaa      180

acgcaagaca gtgaaacgct ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa      240

gagctgccgc tcgatgccag ttgggacacc atttacaccg ctggtacacc atccggcacc      300

agcggtgact acattgacga cgagctgccg gtctaccagg gcgaaacgac gcagaccttg      360

caggattacc tgatgtggca ggttgagctg acaccgtttt ccgacactga ccgcgctatt      420

gctacctcta tcgtcgatgc cgttgatgaa accggttatc tgactgtccc gctggaagat      480

attctcgaaa gtataggcga tgaagagatc gacatcgacg aagttgaagc cgtccttaag      540

cggatccaac ggtttgatcc ggtcggtgtg gcggcaaaag atctgcgtga ctgtctgctg      600

atccaactct cccaattcga taagaccacg ccgtggctgg aagaggccag actgatcatt      660

agcgatcatc tcgatctgtt agccaatcac gacttccgca ctttaatgcg cgtcacgcgt      720

ctgaaagaag atgtgctgaa agaagccgtc aatctgatcc agtcgctcga tccgcgcccc      780

gggcagtcga tccagactgg cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag      840

cataacggtc actggacggt agaactcaac agtgacagca ttccgcgtct gcaaatcaac      900

cagcactacg cctcgatgtg caataacgcg cgcaacgatg gtgacagcca gtttatccgc      960

agcaatctgc aggatgccaa atggttgatc aagagtctgg aaagccgtaa cgatacgcta     1020

ctgcgcgtga gtcgctgtat cgttgaacag cagcaagcct tctttgagca aggtgaagaa     1080

tatatgaaac cgatggtact ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg     1140

atatctcgcg tgaccacgca aaaatacctg catagtccac gaggcatttt tgaactgaag     1200

tatttctttt ccagtcacgt caataccgag ggcggcggcg aagcttcctc cacggcgatt     1260

cgtgcgctgg tgaagaaatt aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc     1320

aagttaacct ctttgctgtc ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag     1380

taccgagagt ctttatccat tccgccgtca aaccagcgta aacaactcgt ttga           1434


<210>  3
<211>  1494
<212>  DNA
<213>  Pseudomonas putida

<400>  3
ctacatcagt cgcttgcgtt cgctcgacgg tgcgatgccg agggactcgc ggtacttggc       60

gacggtgcga cgggctacct ggatgccttg tgcctccagt aaaccagcga tcttgctgtc      120

actcaatggc tttttctgat tttccgccgc aaccagtttc ttgatgatcg cgcggatcgc      180

cgtggacgag cattctccgc cttcggaggt gctgacatgg ctggagaaaa agtatttcag      240

ttcgtagatg ccacgcgggg tgtgcatgta tttctgcgtg gtaacccgcg aaatggtcga      300

ttcgtgcatg cctacggctt cggcaatgtc atgcagtacc aacggcttca tcgcttcgtc      360

gccgtggtcg aggaagccgc gctggtgctc gacgatctgc gtggcaacct tcatcagggt      420

ttcgttacgg ctttgcaggc tcttgatgaa ccagcgcgct tcctgcagct ggttgcgcat      480

gaaggtgttg tcggcgctgg tgtcggcacg gcgcacgaag ccggcgtatt gtgggttgac      540

gcgcaggcgt gggatggctt cctggttcag ttctaccagc caacggtcgc tgtccttgcg      600

cacgatgacg tcgggcacca cgtactcggg ctcgctggac tcgatctgcg aaccagggcg      660

cgggttaagg ctttgcacca gttcgatgac ctggcgcagc tcgtcttcct tgattttcat      720

ccgccgcatc agctggctgt agtcacggct gccgagcagg tcgatgaaat cggtgaccag      780

gcgcttggct tcggtcatcc acggagttgt agcgggcagc tggcgcagtt gcagcaacag      840

gcattcgccg aggttgcggg cgccaacgcc agccggctcg aactgctgga tgcggtgcag      900

taccgcttcg acctcgtcca gttcgatatc cagctccggg tcgaaaccgg cgcagatttc      960

ttcgagtgtg tcttccaggt agccctggcc gttgatgctg tcgatcaggg tcacggcaat     1020

caagcggtcg gtgtcggaca tcggtgccag gttcaactgc cacagcaggt ggctttgcag     1080

gctttcgccg gccgatgtgc gcgtggtgaa gtcccattcg tcgtcatcgt tgctcggcag     1140

gctgctggcg ctggtctggt agatgtcttc ccaagcggta tcgaccggga gctcgttggg     1200

gatgcgctcg ctccactcac cgtcttccag gttgtcggcg ctgacggtgc tttcctggaa     1260

actgttgtcc tgaacttcgg cggccggctt gttctcggcg ttgtcggcca tcgggtcgct     1320

gttgtcgaag tcttcgccgt cttcctgacg ttcgagcatc gggttcgact ccagcgcttc     1380

ctggatttcc tgttggaggt ccagggtaga aagctggagc aggcggatgg cctgttgcaa     1440

ctgaggtgtc atcgtcagtt gctggcccat ttttaggacg agcgatggtt tcat           1494


<210>  4
<211>  16
<212>  DNA
<213>  Myxococcus xanthus


<220>
<221>  misc_feature
<222>  (8)..(11)
<223>  n is a, c, g, or t

<400>  4
tggcacgnnn nttgcw                                                       16


<210>  5
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gacgagatct gttggaaggt cggcaactgg                                        30


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
cttaagatct tccgtgaagt actggcgcac                                        30


<210>  7
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
tgcgcatatg gcgatggaac tgaaacaaag c                                      31


<210>  8
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
tgcggaattc tcagtagtac cgcttgcgct t                                      31


<210>  9
<211>  3444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  9
gcatgccctt gtcctccggt gttgtggtct gcggtgggcg cgctcgcgcg gcgcccgtcc       60

ggccgggcgg acccaggcgc tgcctcgccc gtcccgtacg ccccagcctg caagggcccg      120

ttcgaggctg gaccgaccgc ggttggggcg gtctccgtgc ggcgcggcgg ggcgatcacg      180

gaccgaccac ggctcgaaga acggtcgaag accggctgcc acgctcacgc cgagcggccg      240

cccccagccc ggccgctcta gaaataattt tgtttaactt taagaaggag atataccatg      300

ggcagcgcta gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat      360

atggctaatg accatgatta cggattcact ggcgtcgttt tacaacgtcg tgactgggaa      420

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cactggcgta      480

atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat      540

ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc      600

ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc      660

ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga      720

atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc      780

agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct      840

gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac      900

gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg      960

aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac     1020

cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg     1080

ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag     1140

tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa     1200

ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc     1260

cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg     1320

ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg     1380

aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg     1440

agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc     1500

tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt     1560

ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc     1620

acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg     1680

aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc     1740

tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg     1800

tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata     1860

ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat     1920

ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat     1980

acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc     2040

agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat     2100

atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg     2160

atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga     2220

cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag     2280

tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc     2340

tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac     2400

agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag     2460

tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc     2520

agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc     2580

cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat     2640

ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga     2700

cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag     2760

cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg     2820

ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga     2880

ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga     2940

ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc     3000

atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc     3060

tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct     3120

gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc     3180

gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca     3240

acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg     3300

cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact     3360

cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt     3420

tggtctggtg tcaaaaataa gctt                                            3444


<210>  10
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  10
mgngargcnn wnsmnatgga yccncarcan mg                                     32


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  11
ggrtcnccna rnswngtncc ngtnccrtg                                         29


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is a, c, g, or t

<400>  12
ggwcdachgg haancchaar gg                                                22


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<400>  13
ggcakccaty tygccargtc ncckgt                                            26


