
                                SEQUENCE LISTING

<110> Cleveland Clinic Foundation, The
      Robert H. Silverman,
      Klein, Eric A.
      Weight, Christopher J.
      Nguyen, Carvell T.
      Gupta, Jaydip Das

<120> METHOD FOR DETECTION OF XMRV
  

<130> 3786.1043002

<150> 61/203,556          
<151> 2008-12-23  

<160> 39

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Q445F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag G1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 1
ggactttttg gagtggcttt gtt                                         23

<210> 2
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> Q538R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag G1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 2
gcgtaaaacc gaaagcaaaa t                                           21

<210> 3
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> 480F, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 3
acagagacac ttcccgcccc cg                                          22

<210> 4
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> 625F, forward primerfor a xenotropic murine
      leukemia virus related virus (XMRV) gag G2
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 4
gtaactaccc ctctgagtct aacct                                       25

<210> 5
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> 708R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag G2
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 5
cttcttgaca tccacagact ggtt                                        24

<210> 6
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> 668F, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 6
tccagcgcat tgcatc                                                 16

<210> 7
<211> 26
<212> DNA
<213> Unknown

<220> 
<223> 797F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag G3
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 7
ctcaggtcaa gtctagagtg ttttgt                                      26

<210> 8
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> 874R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag G3
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 8
cctcccaggt gacgatatat gg                                          22

<210> 9
<211> 14
<212> DNA
<213> Unknown

<220> 
<223> 834F, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 9
ccccacggac accc                                                   14

<210> 10
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> 4843F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag pol P1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 10
cgggacagaa ctatccagta tgtga                                       25

<210> 11
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> 4912R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag pol P1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 11
tggctttgct ggcatttact tg                                          22

<210> 12
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> 4873F, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 12
acctgcaccg cctgtg                                                 16

<210> 13
<211> 18
<212> DNA
<213> Unknown

<220> 
<223> 6124F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 13
ggccgagaga gggctact                                               18

<210> 14
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> 6197R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E1
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 14
tgatgatgat ggcttccagt atgc                                        24

<210> 15
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> 6159R, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 15
cacatcccca tttgcc                                                 16

<210> 16
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> ENV-F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E2
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 16
ccctagtggc caccaaacaa                                             20

<210> 17
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> ENV-R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E2
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 17
aaggccccaa ggtctgtatg t                                           21

<210> 18
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> 7192F, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 18
tcgagcagct ccaggcagcc a                                           21

<210> 19
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> ENV-F, forward primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E3
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 19
tcaggacaag ggtggtttga g                                           21

<210> 20
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> ENV-R, reverse primer for a xenotropic murine
      leukemia virus related virus (XMRV) gag env E3
      sequence used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 20
ggcccataat ggtggatatc a                                           21

<210> 21
<211> 27
<212> DNA
<213> Unknown

<220> 
<223> 7480, probe used in the quantitative real time
      polymerase chain reaction study of XMRV

<400> 21
ttaacaggtc cccatggttc acgacca                                     27

<210> 22
<211> 28
<212> DNA
<213> Unknown

<220> 
<223> 6200R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 22
cccatgatga tgatggcttc cagtatgc                                    28

<210> 23
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> 5922F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 23
gctaatgcta cctccctcct gg                                          22

<210> 24
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> 350F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 24
gagttcgtat tcccggccgc agc                                         23

<210> 25
<211> 28
<212> DNA
<213> Unknown

<220> 
<223> 718R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 25
ggtaacccag cgcctcttct tgacatcc                                    28

<210> 26
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> 5942F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 26
ggggacgatg acagacactt tcc                                         23

<210> 27
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> 6159R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 27
cacatcccca tttgccacag tag                                         23

<210> 28
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> 424F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 28
atcagttaac ctacccgagt cggac                                       25

<210> 29
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> 535R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 29
ggtttcggcg taaaaccgaa agc                                         23

<210> 30
<211> 26
<212> DNA
<213> Unknown

<220> 
<223> 6273R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 30
ggagcccact gaggaatcaa aacagg                                      26

<210> 31
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> 5922F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 31
gctaatgcta cctccctcct gg                                          22

<210> 32
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> 350F, forward primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 32
gagttcgtat tcccggccgc agc                                         23

<210> 33
<211> 28
<212> DNA
<213> Unknown

<220> 
<223> 718R, reverse primer used in the Tth-nested real
      time polymerase chain reaction for xenotropic
      murine virus (XMRV)

<400> 33
ggtaacccag cgcctcttct tgacatcc                                    28

<210> 34
<211> 38
<212> DNA
<213> Unknown

<220> 
<223> a 112 nts band amplified with forward primer 424
      and reverse primer 535R

<400> 34
atcagttaac ctacccgagt cggacttttt ggagtggc                         38

<210> 35
<211> 36
<212> DNA
<213> Unknown

<220> 
<223> a 112 nts band amplified with forward primer 424
      and reverse primer 535R

<400> 35
tttgttgggg gacgagagac agagacactt cccgcc                           36

<210> 36
<211> 38
<212> DNA
<213> Unknown

<220> 
<223> a 112 nts band amplified with forward primer 424
      and reverse primer 535R

<400> 36
cccgtctgaa tttttgcttt cggttttacg ccgaaacc                         38

<210> 37
<211> 80
<212> DNA
<213> Unknown

<220> 
<223> a 218 nts band amplified with forward primer 5942F
      and reverse primer 6159R

<400> 37
ggggacgatg acagacactt tccctaaact atattttgac ttgtgtgatt tagttggaga 60
caactgggat gacccggaac                                             80

<210> 38
<211> 80
<212> DNA
<213> Unknown

<220> 
<223> a 218 nts band amplified with forward primer 5942F
      and reverse primer 6159R

<400> 38
ccgatattgg agatggttgc cgctctcccg ggggaagaaa aaggacaaga ctatatgatt 60
tctatgtttg ccccggtcat                                             80

<210> 39
<211> 58
<212> DNA
<213> Unknown

<220> 
<223> a 218 nts band amplified with forward primer 5942F
      and reverse primer 6159R

<400> 39
actgtattaa cagggtgtgg agggccgaga gagggctact gtggcaaatg gggatgtg   58
