                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Liao, James C
       Atsumi, Shota
 
<120>  CONVERSION OF CO2 TO HIGHER ALCOHOLS USING PHOTOSYNTHETIC 
       MICROORGANISMS

<130>  00011-016WO1

<140>  Not yet assigned
<141>  2009-12-18

<150>  US 61/139,593
<151>  2008-12-20

<150>  US 61/219,322
<151>  2009-06-22

<160>  73    

<170>  PatentIn version 3.5

<210>  1
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  1
gccaccggtc tccaattcta tacagtagga gattacctat tag                         43


<210>  2
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  2
cgggatcctt atttagaagt gtcaacaacg tat                                    33


<210>  3
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  3
ggcgagctcc gatcgctttg ggacttggaa cggt                                   34


<210>  4
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  4
ggcgagctca aatcaccagc tgaaacggtg aagt                                   34


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  5
cgcctaggaa ccgttcctgc gcgatcgctc tta                                    33


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  6
cgcctaggta agcgggccac ggcagcgaaa ggg                                    33


<210>  7
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  7
cgggatcctt atttagtaaa atcaatgacc att                                    33


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  8
cgggatcctt agcgggcggc ttcgtatata cgg                                    33


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  9
cgggatcctt atgatttatt ttgttcagca aat                                    33


<210>  10
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  10
gagtggcaat tgatgcccaa gacgcaatct gccgcag                                37


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  11
ggtatatctc cttcttttag agcttgtcca tcgtttcgaa t                           41


<210>  12
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  12
aacgatggac aagctctaaa agaaggagat ataccatgaa aactctgccc aaagagcgtc       60


<210>  13
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  13
cgggatcctt agtagcggcc gggacgatga acg                                    33


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  14
ggaagatctt tcgtgtcgct caaggcgcac tccc                                   34


<210>  15
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  15
ggaagatctg tcttgccacg ccgagcacct ggtc                                   34


<210>  16
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  16
cgggatccga tatctggcga aaatgagacg ttg                                    33


<210>  17
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  17
gggcctgcag gatatcaaat tacgccccgc cctgc                                  35


<210>  18
<211>  1647
<212>  DNA
<213>  Lactococcus lactis


<220>
<221>  CDS
<222>  (1)..(1647)

<400>  18
atg tat aca gta gga gat tac cta tta gac cga tta cac gag tta gga         48
Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly           
1               5                   10                  15                

att gaa gaa att ttt gga gtc cct gga gac tat aac tta caa ttt tta         96
Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu           
            20                  25                  30                    

gat caa att att tcc cgc aag gat atg aaa tgg gtc gga aat gct aat        144
Asp Gln Ile Ile Ser Arg Lys Asp Met Lys Trp Val Gly Asn Ala Asn           
        35                  40                  45                        

gaa tta aat gct tca tat atg gct gat ggc tat gct cgt act aaa aaa        192
Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys           
    50                  55                  60                            

gct gcc gca ttt ctt aca acc ttt gga gta ggt gaa ttg agt gca gtt        240
Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val           
65                  70                  75                  80            

aat gga tta gca gga agt tac gcc gaa aat tta cca gta gta gaa ata        288
Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile           
                85                  90                  95                

gtg gga tca cct aca tca aaa gtt caa aat gaa gga aaa ttt gtt cat        336
Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His           
            100                 105                 110                   

cat acg ctg gct gac ggt gat ttt aaa cac ttt atg aaa atg cac gaa        384
His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu           
        115                 120                 125                       

cct gtt aca gca gct cga act tta ctg aca gca gaa aat gca acc gtt        432
Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val           
    130                 135                 140                           

gaa att gac cga gta ctt tct gca cta tta aaa gaa aga aaa cct gtc        480
Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val           
145                 150                 155                 160           

tat atc aac tta cca gtt gat gtt gct gct gca aaa gca gag aaa ccc        528
Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro           
                165                 170                 175               

tca ctc cct ttg aaa aaa gaa aac tca act tca aat aca agt gac caa        576
Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln           
            180                 185                 190                   

gag atc ttg aac aaa att caa gaa agc ttg aaa aat gcc aaa aaa cca        624
Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro           
        195                 200                 205                       

atc gtg att aca gga cat gaa ata att agt ttt ggc tta gaa aaa aca        672
Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr           
    210                 215                 220                           

gtc tct caa ttt att tca aag aca aaa cta cct att acg aca tta aac        720
Val Ser Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn           
225                 230                 235                 240           

ttt gga aaa agt tca gtt gat gaa gct ctc cct tca ttt tta gga atc        768
Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile           
                245                 250                 255               

tat aat ggt aaa ctc tca gag cct aat ctt aaa gaa ttc gtg gaa tca        816
Tyr Asn Gly Lys Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser           
            260                 265                 270                   

gcc gac ttc atc ctg atg ctt gga gtt aaa ctc aca gac tct tca aca        864
Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr           
        275                 280                 285                       

gga gcc ttc act cat cat tta aat gaa aat aaa atg att tca ctg aat        912
Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn           
    290                 295                 300                           

ata gat gaa gga aaa ata ttt aac gaa agc atc caa aat ttt gat ttt        960
Ile Asp Glu Gly Lys Ile Phe Asn Glu Ser Ile Gln Asn Phe Asp Phe           
305                 310                 315                 320           

gaa tcc ctc atc tcc tct ctc tta gac cta agc gaa ata gaa tac aaa       1008
Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys           
                325                 330                 335               

gga aaa tat atc gat aaa aag caa gaa gac ttt gtt cca tca aat gcg       1056
Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala           
            340                 345                 350                   

ctt tta tca caa gac cgc cta tgg caa gca gtt gaa aac cta act caa       1104
Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln           
        355                 360                 365                       

agc aat gaa aca atc gtt gct gaa caa ggg aca tca ttc ttt ggc gct       1152
Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala           
    370                 375                 380                           

tca tca att ttc tta aaa cca aag agt cat ttt att ggt caa ccc tta       1200
Ser Ser Ile Phe Leu Lys Pro Lys Ser His Phe Ile Gly Gln Pro Leu           
385                 390                 395                 400           

tgg gga tca att gga tat aca ttc cca gca gca tta gga agc caa att       1248
Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile           
                405                 410                 415               

gca gat aaa gaa agc aga cac ctt tta ttt att ggt gat ggt tca ctt       1296
Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu           
            420                 425                 430                   

caa ctt acg gtg caa gaa tta gga tta gca atc aga gaa aaa att aat       1344
Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn           
        435                 440                 445                       

cca att tgc ttt att atc aat aat gat ggt tat aca gtc gaa aga gaa       1392
Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu           
    450                 455                 460                           

att cat gga cca aat caa agc tac aat gat att cca atg tgg aat tac       1440
Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr           
465                 470                 475                 480           

tca aaa tta cca gaa tca ttt gga gca aca gaa gaa cga gta gtc tcg       1488
Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Glu Arg Val Val Ser           
                485                 490                 495               

aaa atc gtt aga act gaa aat gaa ttt gtg tct gtc atg aaa gaa gct       1536
Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala           
            500                 505                 510                   

caa gca gat cca aat aga atg tac tgg att gag tta att ttg gca aaa       1584
Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys           
        515                 520                 525                       

gaa gat gca cca aaa gta ctg aaa aaa atg ggc aaa cta ttt gct gaa       1632
Glu Asp Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu           
    530                 535                 540                           

caa aat aaa tca taa                                                   1647
Gln Asn Lys Ser                                                           
545                                                                       


<210>  19
<211>  548
<212>  PRT
<213>  Lactococcus lactis

<400>  19

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser Arg Lys Asp Met Lys Trp Val Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln 
            180                 185                 190         


Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro 
        195                 200                 205             


Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Ser Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Lys Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Lys Ile Phe Asn Glu Ser Ile Gln Asn Phe Asp Phe 
305                 310                 315                 320 


Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys 
                325                 330                 335     


Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala 
            340                 345                 350         


Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Ser Ile Phe Leu Lys Pro Lys Ser His Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Glu Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys 
        515                 520                 525             


Glu Asp Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys Ser 
545             


<210>  20
<211>  1692
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1692)

<400>  20
atg tct gaa att act ctt gga aaa tac tta ttt gaa aga ttg aag caa         48
Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln           
1               5                   10                  15                

gtt aat gtt aac acc att ttt ggg cta cca ggc gac ttc aac ttg tcc         96
Val Asn Val Asn Thr Ile Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser           
            20                  25                  30                    

cta ttg gac aag att tac gag gta gat gga ttg aga tgg gct ggt aat        144
Leu Leu Asp Lys Ile Tyr Glu Val Asp Gly Leu Arg Trp Ala Gly Asn           
        35                  40                  45                        

gca aat gag ctg aac gcc gcc tat gcc gcc gat ggt tac gca cgc atc        192
Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile           
    50                  55                  60                            

aag ggt tta tct gtg ctg gta act act ttt ggc gta ggt gaa tta tcc        240
Lys Gly Leu Ser Val Leu Val Thr Thr Phe Gly Val Gly Glu Leu Ser           
65                  70                  75                  80            

gcc ttg aat ggt att gca gga tcg tat gca gaa cac gtc ggt gta ctg        288
Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu           
                85                  90                  95                

cat gtt gtt ggt gtc ccc tct atc tcc gct cag gct aag caa ttg ttg        336
His Val Val Gly Val Pro Ser Ile Ser Ala Gln Ala Lys Gln Leu Leu           
            100                 105                 110                   

ttg cat cat acc ttg ggt aac ggt gat ttt acc gtt ttt cac aga atg        384
Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met           
        115                 120                 125                       

tcc gcc aat atc tca gaa act aca tca atg att aca gac att gct aca        432
Ser Ala Asn Ile Ser Glu Thr Thr Ser Met Ile Thr Asp Ile Ala Thr           
    130                 135                 140                           

gcc cct tca gaa atc gat agg ttg atc agg aca aca ttt ata aca caa        480
Ala Pro Ser Glu Ile Asp Arg Leu Ile Arg Thr Thr Phe Ile Thr Gln           
145                 150                 155                 160           

agg cct agc tac ttg ggg ttg cca gcg aat ttg gta gat cta aag gtt        528
Arg Pro Ser Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Lys Val           
                165                 170                 175               

cct ggt tct ctt ttg gaa aaa ccg att gat cta tca tta aaa cct aac        576
Pro Gly Ser Leu Leu Glu Lys Pro Ile Asp Leu Ser Leu Lys Pro Asn           
            180                 185                 190                   

gat ccc gaa gct gaa aag gaa gtt att gat acc gta cta gaa ttg atc        624
Asp Pro Glu Ala Glu Lys Glu Val Ile Asp Thr Val Leu Glu Leu Ile           
        195                 200                 205                       

cag aat tcg aaa aac cct gtt ata cta tcg gat gcc tgt gct tct agg        672
Gln Asn Ser Lys Asn Pro Val Ile Leu Ser Asp Ala Cys Ala Ser Arg           
    210                 215                 220                           

cac aac gtt aaa aaa gaa acc cag aag tta att gat ttg acg caa ttc        720
His Asn Val Lys Lys Glu Thr Gln Lys Leu Ile Asp Leu Thr Gln Phe           
225                 230                 235                 240           

cca gct ttt gtg aca cct cta ggt aaa ggg tca ata gat gaa cag cat        768
Pro Ala Phe Val Thr Pro Leu Gly Lys Gly Ser Ile Asp Glu Gln His           
                245                 250                 255               

ccc aga tat ggc ggt gtt tat gtg gga acg ctg tcc aaa caa gac gtg        816
Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Gln Asp Val           
            260                 265                 270                   

aaa cag gcc gtt gag tcg gct gat ttg atc ctt tcg gtc ggt gct ttg        864
Lys Gln Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu           
        275                 280                 285                       

ctc tct gat ttt aac aca ggt tcg ttt tcc tac tcc tac aag act aaa        912
Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys           
    290                 295                 300                           

aat gta gtg gag ttt cat tcc gat tac gta aag gtg aag aac gct acg        960
Asn Val Val Glu Phe His Ser Asp Tyr Val Lys Val Lys Asn Ala Thr           
305                 310                 315                 320           

ttc ctc ggt gta caa atg aaa ttt gca cta caa aac tta ctg aag gtt       1008
Phe Leu Gly Val Gln Met Lys Phe Ala Leu Gln Asn Leu Leu Lys Val           
                325                 330                 335               

att ccc gat gtt gtt aag ggc tac aag agc gtt ccc gta cca acc aaa       1056
Ile Pro Asp Val Val Lys Gly Tyr Lys Ser Val Pro Val Pro Thr Lys           
            340                 345                 350                   

act ccc gca aac aaa ggt gta cct gct agc acg ccc ttg aaa caa gag       1104
Thr Pro Ala Asn Lys Gly Val Pro Ala Ser Thr Pro Leu Lys Gln Glu           
        355                 360                 365                       

tgg ttg tgg aac gaa ttg tcc aaa ttc ttg caa gaa ggt gat gtt atc       1152
Trp Leu Trp Asn Glu Leu Ser Lys Phe Leu Gln Glu Gly Asp Val Ile           
    370                 375                 380                           

att tcc gag acc ggc acg tct gcc ttc ggt atc aat caa act atc ttt       1200
Ile Ser Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Ile Phe           
385                 390                 395                 400           

cct aag gac gcc tac ggt atc tcg cag gtg ttg tgg ggg tcc atc ggt       1248
Pro Lys Asp Ala Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly           
                405                 410                 415               

ttt aca aca gga gca act tta ggt gct gcc ttt gcc gct gag gag att       1296
Phe Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile           
            420                 425                 430                   

gac ccc aac aag aga gtc atc tta ttc ata ggt gac ggg tct ttg cag       1344
Asp Pro Asn Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln           
        435                 440                 445                       

tta acc gtc caa gaa atc tcc acc atg atc aga tgg ggg tta aag ccg       1392
Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro           
    450                 455                 460                           

tat ctt ttt gtc ctt aac aac gac ggc tac act atc gaa aag ctg att       1440
Tyr Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile           
465                 470                 475                 480           

cat ggg cct cac gca gag tac aac gaa atc cag acc tgg gat cac ctc       1488
His Gly Pro His Ala Glu Tyr Asn Glu Ile Gln Thr Trp Asp His Leu           
                485                 490                 495               

gcc ctg ttg ccc gca ttt ggt gcg aaa aag tac gaa aat cac aag atc       1536
Ala Leu Leu Pro Ala Phe Gly Ala Lys Lys Tyr Glu Asn His Lys Ile           
            500                 505                 510                   

gcc act acg ggt gag tgg gat gcc tta acc act gat tca gag ttc cag       1584
Ala Thr Thr Gly Glu Trp Asp Ala Leu Thr Thr Asp Ser Glu Phe Gln           
        515                 520                 525                       

aaa aac tcg gtg atc aga cta att gaa ctg aaa ctg ccc gtc ttt gat       1632
Lys Asn Ser Val Ile Arg Leu Ile Glu Leu Lys Leu Pro Val Phe Asp           
    530                 535                 540                           

gct ccg gaa agt ttg atc aaa caa gcg caa ttg act gcc gct aca aat       1680
Ala Pro Glu Ser Leu Ile Lys Gln Ala Gln Leu Thr Ala Ala Thr Asn           
545                 550                 555                 560           

gcc aaa caa taa                                                       1692
Ala Lys Gln                                                               
                                                                          


<210>  21
<211>  563
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  21

Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln 
1               5                   10                  15      


Val Asn Val Asn Thr Ile Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser 
            20                  25                  30          


Leu Leu Asp Lys Ile Tyr Glu Val Asp Gly Leu Arg Trp Ala Gly Asn 
        35                  40                  45              


Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile 
    50                  55                  60                  


Lys Gly Leu Ser Val Leu Val Thr Thr Phe Gly Val Gly Glu Leu Ser 
65                  70                  75                  80  


Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu 
                85                  90                  95      


His Val Val Gly Val Pro Ser Ile Ser Ala Gln Ala Lys Gln Leu Leu 
            100                 105                 110         


Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met 
        115                 120                 125             


Ser Ala Asn Ile Ser Glu Thr Thr Ser Met Ile Thr Asp Ile Ala Thr 
    130                 135                 140                 


Ala Pro Ser Glu Ile Asp Arg Leu Ile Arg Thr Thr Phe Ile Thr Gln 
145                 150                 155                 160 


Arg Pro Ser Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Lys Val 
                165                 170                 175     


Pro Gly Ser Leu Leu Glu Lys Pro Ile Asp Leu Ser Leu Lys Pro Asn 
            180                 185                 190         


Asp Pro Glu Ala Glu Lys Glu Val Ile Asp Thr Val Leu Glu Leu Ile 
        195                 200                 205             


Gln Asn Ser Lys Asn Pro Val Ile Leu Ser Asp Ala Cys Ala Ser Arg 
    210                 215                 220                 


His Asn Val Lys Lys Glu Thr Gln Lys Leu Ile Asp Leu Thr Gln Phe 
225                 230                 235                 240 


Pro Ala Phe Val Thr Pro Leu Gly Lys Gly Ser Ile Asp Glu Gln His 
                245                 250                 255     


Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Gln Asp Val 
            260                 265                 270         


Lys Gln Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu 
        275                 280                 285             


Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys 
    290                 295                 300                 


Asn Val Val Glu Phe His Ser Asp Tyr Val Lys Val Lys Asn Ala Thr 
305                 310                 315                 320 


Phe Leu Gly Val Gln Met Lys Phe Ala Leu Gln Asn Leu Leu Lys Val 
                325                 330                 335     


Ile Pro Asp Val Val Lys Gly Tyr Lys Ser Val Pro Val Pro Thr Lys 
            340                 345                 350         


Thr Pro Ala Asn Lys Gly Val Pro Ala Ser Thr Pro Leu Lys Gln Glu 
        355                 360                 365             


Trp Leu Trp Asn Glu Leu Ser Lys Phe Leu Gln Glu Gly Asp Val Ile 
    370                 375                 380                 


Ile Ser Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Ile Phe 
385                 390                 395                 400 


Pro Lys Asp Ala Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly 
                405                 410                 415     


Phe Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile 
            420                 425                 430         


Asp Pro Asn Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln 
        435                 440                 445             


Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro 
    450                 455                 460                 


Tyr Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile 
465                 470                 475                 480 


His Gly Pro His Ala Glu Tyr Asn Glu Ile Gln Thr Trp Asp His Leu 
                485                 490                 495     


Ala Leu Leu Pro Ala Phe Gly Ala Lys Lys Tyr Glu Asn His Lys Ile 
            500                 505                 510         


Ala Thr Thr Gly Glu Trp Asp Ala Leu Thr Thr Asp Ser Glu Phe Gln 
        515                 520                 525             


Lys Asn Ser Val Ile Arg Leu Ile Glu Leu Lys Leu Pro Val Phe Asp 
    530                 535                 540                 


Ala Pro Glu Ser Leu Ile Lys Gln Ala Gln Leu Thr Ala Ala Thr Asn 
545                 550                 555                 560 


Ala Lys Gln 
            


<210>  22
<211>  1908
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1908)

<400>  22
atg gca cct gtt aca att gaa aag ttc gta aat caa gaa gaa cga cac         48
Met Ala Pro Val Thr Ile Glu Lys Phe Val Asn Gln Glu Glu Arg His           
1               5                   10                  15                

ctt gtt tcc aac cga tca gca aca att ccg ttt ggt gaa tac ata ttt         96
Leu Val Ser Asn Arg Ser Ala Thr Ile Pro Phe Gly Glu Tyr Ile Phe           
            20                  25                  30                    

aaa aga ttg ttg tcc atc gat acg aaa tca gtt ttc ggt gtt cct ggt        144
Lys Arg Leu Leu Ser Ile Asp Thr Lys Ser Val Phe Gly Val Pro Gly           
        35                  40                  45                        

gac ttc aac tta tct cta tta gaa tat ctc tat tca cct agt gtt gaa        192
Asp Phe Asn Leu Ser Leu Leu Glu Tyr Leu Tyr Ser Pro Ser Val Glu           
    50                  55                  60                            

tca gct ggc cta aga tgg gtc ggc acg tgt aat gaa ctg aac gcc gct        240
Ser Ala Gly Leu Arg Trp Val Gly Thr Cys Asn Glu Leu Asn Ala Ala           
65                  70                  75                  80            

tat gcg gcc gac gga tat tcc cgt tac tct aat aag att ggc tgt tta        288
Tyr Ala Ala Asp Gly Tyr Ser Arg Tyr Ser Asn Lys Ile Gly Cys Leu           
                85                  90                  95                

ata acc acg tat ggc gtt ggt gaa tta agc gcc ttg aac ggt ata gcc        336
Ile Thr Thr Tyr Gly Val Gly Glu Leu Ser Ala Leu Asn Gly Ile Ala           
            100                 105                 110                   

ggt tcg ttc gct gaa aat gtc aaa gtt ttg cac att gtt ggt gtg gcc        384
Gly Ser Phe Ala Glu Asn Val Lys Val Leu His Ile Val Gly Val Ala           
        115                 120                 125                       

aag tcc ata gat tcg cgt tca agt aac ttt agt gat cgg aac cta cat        432
Lys Ser Ile Asp Ser Arg Ser Ser Asn Phe Ser Asp Arg Asn Leu His           
    130                 135                 140                           

cat ttg gtc cca cag cta cat gat tca aat ttt aaa ggg cca aat cat        480
His Leu Val Pro Gln Leu His Asp Ser Asn Phe Lys Gly Pro Asn His           
145                 150                 155                 160           

aaa gta tat cat gat atg gta aaa gat aga gtc gct tgc tcg gta gcc        528
Lys Val Tyr His Asp Met Val Lys Asp Arg Val Ala Cys Ser Val Ala           
                165                 170                 175               

tac ttg gag gat att gaa act gca tgt gac caa gtc gat aat gtt atc        576
Tyr Leu Glu Asp Ile Glu Thr Ala Cys Asp Gln Val Asp Asn Val Ile           
            180                 185                 190                   

cgc gat att tac aag tat tct aaa cct ggt tat att ttt gtt cct gca        624
Arg Asp Ile Tyr Lys Tyr Ser Lys Pro Gly Tyr Ile Phe Val Pro Ala           
        195                 200                 205                       

gat ttt gcg gat atg tct gtt aca tgt gat aat ttg gtt aat gtt cca        672
Asp Phe Ala Asp Met Ser Val Thr Cys Asp Asn Leu Val Asn Val Pro           
    210                 215                 220                           

cgt ata tct caa caa gat tgt ata gta tac cct tct gaa aac caa ttg        720
Arg Ile Ser Gln Gln Asp Cys Ile Val Tyr Pro Ser Glu Asn Gln Leu           
225                 230                 235                 240           

tct gac ata atc aac aag att act agt tgg ata tat tcc agt aaa aca        768
Ser Asp Ile Ile Asn Lys Ile Thr Ser Trp Ile Tyr Ser Ser Lys Thr           
                245                 250                 255               

cct gcg atc ctt gga gac gta ctg act gat agg tat ggt gtg agt aac        816
Pro Ala Ile Leu Gly Asp Val Leu Thr Asp Arg Tyr Gly Val Ser Asn           
            260                 265                 270                   

ttt ttg aac aag ctt atc tgc aaa act ggg att tgg aat ttt tcc act        864
Phe Leu Asn Lys Leu Ile Cys Lys Thr Gly Ile Trp Asn Phe Ser Thr           
        275                 280                 285                       

gtt atg gga aaa tct gta att gat gag tca aac cca act tat atg ggt        912
Val Met Gly Lys Ser Val Ile Asp Glu Ser Asn Pro Thr Tyr Met Gly           
    290                 295                 300                           

caa tat aat ggt aaa gaa ggt tta aaa caa gtc tat gaa cat ttt gaa        960
Gln Tyr Asn Gly Lys Glu Gly Leu Lys Gln Val Tyr Glu His Phe Glu           
305                 310                 315                 320           

ctg tgc gac ttg gtc ttg cat ttt gga gtc gac atc aat gaa att aat       1008
Leu Cys Asp Leu Val Leu His Phe Gly Val Asp Ile Asn Glu Ile Asn           
                325                 330                 335               

aat ggg cat tat act ttt act tat aaa cca aat gct aaa atc att caa       1056
Asn Gly His Tyr Thr Phe Thr Tyr Lys Pro Asn Ala Lys Ile Ile Gln           
            340                 345                 350                   

ttt cat ccg aat tat att cgc ctt gtg gac act agg cag ggc aat gag       1104
Phe His Pro Asn Tyr Ile Arg Leu Val Asp Thr Arg Gln Gly Asn Glu           
        355                 360                 365                       

caa atg ttc aaa gga atc aat ttt gcc cct att tta aaa gaa cta tac       1152
Gln Met Phe Lys Gly Ile Asn Phe Ala Pro Ile Leu Lys Glu Leu Tyr           
    370                 375                 380                           

aag cgc att gac gtt tct aaa ctt tct ttg caa tat gat tca aat gta       1200
Lys Arg Ile Asp Val Ser Lys Leu Ser Leu Gln Tyr Asp Ser Asn Val           
385                 390                 395                 400           

act caa tat acg aac gaa aca atg cgg tta gaa gat cct acc aat gga       1248
Thr Gln Tyr Thr Asn Glu Thr Met Arg Leu Glu Asp Pro Thr Asn Gly           
                405                 410                 415               

caa tca agc att att aca caa gtt cac tta caa aag acg atg cct aaa       1296
Gln Ser Ser Ile Ile Thr Gln Val His Leu Gln Lys Thr Met Pro Lys           
            420                 425                 430                   

ttt ttg aac cct ggt gat gtt gtc gtt tgt gaa aca ggc tct ttt caa       1344
Phe Leu Asn Pro Gly Asp Val Val Val Cys Glu Thr Gly Ser Phe Gln           
        435                 440                 445                       

ttc tct gtt cgt gat ttc gcg ttt cct tcg caa tta aaa tat ata tcg       1392
Phe Ser Val Arg Asp Phe Ala Phe Pro Ser Gln Leu Lys Tyr Ile Ser           
    450                 455                 460                           

caa gga ttt ttc ctt tcc att ggc atg gcc ctt cct gcc gcc cta ggt       1440
Gln Gly Phe Phe Leu Ser Ile Gly Met Ala Leu Pro Ala Ala Leu Gly           
465                 470                 475                 480           

gtt gga att gcc atg caa gac cac tca aac gct cac atc aat ggt ggc       1488
Val Gly Ile Ala Met Gln Asp His Ser Asn Ala His Ile Asn Gly Gly           
                485                 490                 495               

aac gta aaa gag gac tat aag cca aga tta att ttg ttt gaa ggt gac       1536
Asn Val Lys Glu Asp Tyr Lys Pro Arg Leu Ile Leu Phe Glu Gly Asp           
            500                 505                 510                   

ggt gca gca cag atg aca atc caa gaa ctg agc acc att ctg aag tgc       1584
Gly Ala Ala Gln Met Thr Ile Gln Glu Leu Ser Thr Ile Leu Lys Cys           
        515                 520                 525                       

aat att cca cta gaa gtt atc att tgg aac aat aac ggc tac act att       1632
Asn Ile Pro Leu Glu Val Ile Ile Trp Asn Asn Asn Gly Tyr Thr Ile           
    530                 535                 540                           

gaa aga gcc atc atg ggc cct acc agg tcg tat aac gac gtt atg tct       1680
Glu Arg Ala Ile Met Gly Pro Thr Arg Ser Tyr Asn Asp Val Met Ser           
545                 550                 555                 560           

tgg aaa tgg acc aaa cta ttt gaa gca ttc gga gac ttc gac gga aag       1728
Trp Lys Trp Thr Lys Leu Phe Glu Ala Phe Gly Asp Phe Asp Gly Lys           
                565                 570                 575               

tat act aat agc act ctc att caa tgt ccc tct aaa tta gca ctg aaa       1776
Tyr Thr Asn Ser Thr Leu Ile Gln Cys Pro Ser Lys Leu Ala Leu Lys           
            580                 585                 590                   

ttg gag gag ctt aag aat tca aac aaa aga agc ggg ata gaa ctt tta       1824
Leu Glu Glu Leu Lys Asn Ser Asn Lys Arg Ser Gly Ile Glu Leu Leu           
        595                 600                 605                       

gaa gtc aaa tta ggc gaa ttg gat ttc ccc gaa cag cta aag tgc atg       1872
Glu Val Lys Leu Gly Glu Leu Asp Phe Pro Glu Gln Leu Lys Cys Met           
    610                 615                 620                           

gtt gaa gca gcg gca ctt aaa aga aat aaa aaa tag                       1908
Val Glu Ala Ala Ala Leu Lys Arg Asn Lys Lys                               
625                 630                 635                               


<210>  23
<211>  635
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  23

Met Ala Pro Val Thr Ile Glu Lys Phe Val Asn Gln Glu Glu Arg His 
1               5                   10                  15      


Leu Val Ser Asn Arg Ser Ala Thr Ile Pro Phe Gly Glu Tyr Ile Phe 
            20                  25                  30          


Lys Arg Leu Leu Ser Ile Asp Thr Lys Ser Val Phe Gly Val Pro Gly 
        35                  40                  45              


Asp Phe Asn Leu Ser Leu Leu Glu Tyr Leu Tyr Ser Pro Ser Val Glu 
    50                  55                  60                  


Ser Ala Gly Leu Arg Trp Val Gly Thr Cys Asn Glu Leu Asn Ala Ala 
65                  70                  75                  80  


Tyr Ala Ala Asp Gly Tyr Ser Arg Tyr Ser Asn Lys Ile Gly Cys Leu 
                85                  90                  95      


Ile Thr Thr Tyr Gly Val Gly Glu Leu Ser Ala Leu Asn Gly Ile Ala 
            100                 105                 110         


Gly Ser Phe Ala Glu Asn Val Lys Val Leu His Ile Val Gly Val Ala 
        115                 120                 125             


Lys Ser Ile Asp Ser Arg Ser Ser Asn Phe Ser Asp Arg Asn Leu His 
    130                 135                 140                 


His Leu Val Pro Gln Leu His Asp Ser Asn Phe Lys Gly Pro Asn His 
145                 150                 155                 160 


Lys Val Tyr His Asp Met Val Lys Asp Arg Val Ala Cys Ser Val Ala 
                165                 170                 175     


Tyr Leu Glu Asp Ile Glu Thr Ala Cys Asp Gln Val Asp Asn Val Ile 
            180                 185                 190         


Arg Asp Ile Tyr Lys Tyr Ser Lys Pro Gly Tyr Ile Phe Val Pro Ala 
        195                 200                 205             


Asp Phe Ala Asp Met Ser Val Thr Cys Asp Asn Leu Val Asn Val Pro 
    210                 215                 220                 


Arg Ile Ser Gln Gln Asp Cys Ile Val Tyr Pro Ser Glu Asn Gln Leu 
225                 230                 235                 240 


Ser Asp Ile Ile Asn Lys Ile Thr Ser Trp Ile Tyr Ser Ser Lys Thr 
                245                 250                 255     


Pro Ala Ile Leu Gly Asp Val Leu Thr Asp Arg Tyr Gly Val Ser Asn 
            260                 265                 270         


Phe Leu Asn Lys Leu Ile Cys Lys Thr Gly Ile Trp Asn Phe Ser Thr 
        275                 280                 285             


Val Met Gly Lys Ser Val Ile Asp Glu Ser Asn Pro Thr Tyr Met Gly 
    290                 295                 300                 


Gln Tyr Asn Gly Lys Glu Gly Leu Lys Gln Val Tyr Glu His Phe Glu 
305                 310                 315                 320 


Leu Cys Asp Leu Val Leu His Phe Gly Val Asp Ile Asn Glu Ile Asn 
                325                 330                 335     


Asn Gly His Tyr Thr Phe Thr Tyr Lys Pro Asn Ala Lys Ile Ile Gln 
            340                 345                 350         


Phe His Pro Asn Tyr Ile Arg Leu Val Asp Thr Arg Gln Gly Asn Glu 
        355                 360                 365             


Gln Met Phe Lys Gly Ile Asn Phe Ala Pro Ile Leu Lys Glu Leu Tyr 
    370                 375                 380                 


Lys Arg Ile Asp Val Ser Lys Leu Ser Leu Gln Tyr Asp Ser Asn Val 
385                 390                 395                 400 


Thr Gln Tyr Thr Asn Glu Thr Met Arg Leu Glu Asp Pro Thr Asn Gly 
                405                 410                 415     


Gln Ser Ser Ile Ile Thr Gln Val His Leu Gln Lys Thr Met Pro Lys 
            420                 425                 430         


Phe Leu Asn Pro Gly Asp Val Val Val Cys Glu Thr Gly Ser Phe Gln 
        435                 440                 445             


Phe Ser Val Arg Asp Phe Ala Phe Pro Ser Gln Leu Lys Tyr Ile Ser 
    450                 455                 460                 


Gln Gly Phe Phe Leu Ser Ile Gly Met Ala Leu Pro Ala Ala Leu Gly 
465                 470                 475                 480 


Val Gly Ile Ala Met Gln Asp His Ser Asn Ala His Ile Asn Gly Gly 
                485                 490                 495     


Asn Val Lys Glu Asp Tyr Lys Pro Arg Leu Ile Leu Phe Glu Gly Asp 
            500                 505                 510         


Gly Ala Ala Gln Met Thr Ile Gln Glu Leu Ser Thr Ile Leu Lys Cys 
        515                 520                 525             


Asn Ile Pro Leu Glu Val Ile Ile Trp Asn Asn Asn Gly Tyr Thr Ile 
    530                 535                 540                 


Glu Arg Ala Ile Met Gly Pro Thr Arg Ser Tyr Asn Asp Val Met Ser 
545                 550                 555                 560 


Trp Lys Trp Thr Lys Leu Phe Glu Ala Phe Gly Asp Phe Asp Gly Lys 
                565                 570                 575     


Tyr Thr Asn Ser Thr Leu Ile Gln Cys Pro Ser Lys Leu Ala Leu Lys 
            580                 585                 590         


Leu Glu Glu Leu Lys Asn Ser Asn Lys Arg Ser Gly Ile Glu Leu Leu 
        595                 600                 605             


Glu Val Lys Leu Gly Glu Leu Asp Phe Pro Glu Gln Leu Lys Cys Met 
    610                 615                 620                 


Val Glu Ala Ala Ala Leu Lys Arg Asn Lys Lys 
625                 630                 635 


<210>  24
<211>  1830
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1830)

<400>  24
atg aat tct agc tat aca cag aga tat gca ctg ccg aag tgt ata gca         48
Met Asn Ser Ser Tyr Thr Gln Arg Tyr Ala Leu Pro Lys Cys Ile Ala           
1               5                   10                  15                

ata tca gat tat ctt ttc cat cgg ctc aac cag ctg aac ata cat acc         96
Ile Ser Asp Tyr Leu Phe His Arg Leu Asn Gln Leu Asn Ile His Thr           
            20                  25                  30                    

ata ttt gga ctc tcc gga gaa ttt agc atg ccg ttg ctg gat aaa cta        144
Ile Phe Gly Leu Ser Gly Glu Phe Ser Met Pro Leu Leu Asp Lys Leu           
        35                  40                  45                        

tac aac att ccg aac tta cga tgg gcc ggt aat tct aat gag tta aat        192
Tyr Asn Ile Pro Asn Leu Arg Trp Ala Gly Asn Ser Asn Glu Leu Asn           
    50                  55                  60                            

gct gcc tac gca gca gat gga tac tca cga cta aaa ggc ttg gga tgt        240
Ala Ala Tyr Ala Ala Asp Gly Tyr Ser Arg Leu Lys Gly Leu Gly Cys           
65                  70                  75                  80            

ctc ata aca acc ttt ggt gta ggc gaa tta tcg gca atc aat ggc gtg        288
Leu Ile Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile Asn Gly Val           
                85                  90                  95                

gcc gga tct tac gct gaa cat gta gga ata ctt cac ata gtg ggt atg        336
Ala Gly Ser Tyr Ala Glu His Val Gly Ile Leu His Ile Val Gly Met           
            100                 105                 110                   

ccg cca aca agt gca caa acg aaa caa cta cta ctg cat cat act ctg        384
Pro Pro Thr Ser Ala Gln Thr Lys Gln Leu Leu Leu His His Thr Leu           
        115                 120                 125                       

ggc aat ggt gat ttc acg gta ttt cat aga ata gcc agt gat gta gca        432
Gly Asn Gly Asp Phe Thr Val Phe His Arg Ile Ala Ser Asp Val Ala           
    130                 135                 140                           

tgc tat aca aca ttg att att gac tct gaa tta tgt gcc gac gaa gtc        480
Cys Tyr Thr Thr Leu Ile Ile Asp Ser Glu Leu Cys Ala Asp Glu Val           
145                 150                 155                 160           

gat aag tgc atc aaa aag gct tgg ata gaa cag agg cca gta tac atg        528
Asp Lys Cys Ile Lys Lys Ala Trp Ile Glu Gln Arg Pro Val Tyr Met           
                165                 170                 175               

ggc atg cct gtc aac cag gta aat ctc ccg att gaa tca gca agg ctt        576
Gly Met Pro Val Asn Gln Val Asn Leu Pro Ile Glu Ser Ala Arg Leu           
            180                 185                 190                   

aat aca cct ctg gat tta caa ttg cat aaa aac gac cca gac gta gag        624
Asn Thr Pro Leu Asp Leu Gln Leu His Lys Asn Asp Pro Asp Val Glu           
        195                 200                 205                       

aaa gaa gtt att tct cga ata ttg agt ttt ata tac aaa agc cag aat        672
Lys Glu Val Ile Ser Arg Ile Leu Ser Phe Ile Tyr Lys Ser Gln Asn           
    210                 215                 220                           

ccg gca atc atc gta gat gca tgt act agt cga cag aat tta atc gag        720
Pro Ala Ile Ile Val Asp Ala Cys Thr Ser Arg Gln Asn Leu Ile Glu           
225                 230                 235                 240           

gag act aaa gag ctt tgt aat agg ctt aaa ttt cca gtt ttt gtt aca        768
Glu Thr Lys Glu Leu Cys Asn Arg Leu Lys Phe Pro Val Phe Val Thr           
                245                 250                 255               

cct atg ggt aag ggt aca gta aac gaa aca gac ccg caa ttt ggg ggc        816
Pro Met Gly Lys Gly Thr Val Asn Glu Thr Asp Pro Gln Phe Gly Gly           
            260                 265                 270                   

gta ttc acg ggc tcg ata tca gcc cca gaa gta aga gaa gta gtt gat        864
Val Phe Thr Gly Ser Ile Ser Ala Pro Glu Val Arg Glu Val Val Asp           
        275                 280                 285                       

ttt gcc gat ttt atc atc gtc att ggt tgc atg ctc tcc gaa ttc agc        912
Phe Ala Asp Phe Ile Ile Val Ile Gly Cys Met Leu Ser Glu Phe Ser           
    290                 295                 300                           

acg tca act ttc cac ttc caa tat aaa act aag aat tgt gcg cta cta        960
Thr Ser Thr Phe His Phe Gln Tyr Lys Thr Lys Asn Cys Ala Leu Leu           
305                 310                 315                 320           

tat tct aca tct gtg aaa ttg aaa aat gcc aca tat cct gac ttg agc       1008
Tyr Ser Thr Ser Val Lys Leu Lys Asn Ala Thr Tyr Pro Asp Leu Ser           
                325                 330                 335               

att aaa tta cta cta cag aaa ata tta gca aat ctt gat gaa tct aaa       1056
Ile Lys Leu Leu Leu Gln Lys Ile Leu Ala Asn Leu Asp Glu Ser Lys           
            340                 345                 350                   

ctg tct tac caa cca agc gaa caa ccc agt atg atg gtt cca aga cct       1104
Leu Ser Tyr Gln Pro Ser Glu Gln Pro Ser Met Met Val Pro Arg Pro           
        355                 360                 365                       

tac cca gca gga aat gtc ctc ttg aga caa gaa tgg gtc tgg aat gaa       1152
Tyr Pro Ala Gly Asn Val Leu Leu Arg Gln Glu Trp Val Trp Asn Glu           
    370                 375                 380                           

ata tcc cat tgg ttc caa cca ggt gac ata atc ata aca gaa act ggt       1200
Ile Ser His Trp Phe Gln Pro Gly Asp Ile Ile Ile Thr Glu Thr Gly           
385                 390                 395                 400           

gct tct gca ttt gga gtt aac cag acc aga ttt ccg gta aat aca cta       1248
Ala Ser Ala Phe Gly Val Asn Gln Thr Arg Phe Pro Val Asn Thr Leu           
                405                 410                 415               

ggt att tcg caa gct ctt tgg gga tct gtc gga tat aca atg ggg gcg       1296
Gly Ile Ser Gln Ala Leu Trp Gly Ser Val Gly Tyr Thr Met Gly Ala           
            420                 425                 430                   

tgt ctt ggg gca gaa ttt gct gtt caa gag ata aac aag gat aaa ttc       1344
Cys Leu Gly Ala Glu Phe Ala Val Gln Glu Ile Asn Lys Asp Lys Phe           
        435                 440                 445                       

ccc gca act aaa cat aga gtt att ctg ttt atg ggt gac ggt gct ttc       1392
Pro Ala Thr Lys His Arg Val Ile Leu Phe Met Gly Asp Gly Ala Phe           
    450                 455                 460                           

caa ttg aca gtt caa gaa tta tcc aca att gtt aag tgg gga ttg aca       1440
Gln Leu Thr Val Gln Glu Leu Ser Thr Ile Val Lys Trp Gly Leu Thr           
465                 470                 475                 480           

cct tat att ttt gtg atg aat aac caa ggt tac tct gtg gac agg ttt       1488
Pro Tyr Ile Phe Val Met Asn Asn Gln Gly Tyr Ser Val Asp Arg Phe           
                485                 490                 495               

ttg cat cac agg tca gat gct agt tat tac gat atc caa cct tgg aac       1536
Leu His His Arg Ser Asp Ala Ser Tyr Tyr Asp Ile Gln Pro Trp Asn           
            500                 505                 510                   

tac ttg gga tta ttg cga gta ttt ggt tgc acg aac tac gaa acg aaa       1584
Tyr Leu Gly Leu Leu Arg Val Phe Gly Cys Thr Asn Tyr Glu Thr Lys           
        515                 520                 525                       

aaa att att act gtt gga gaa ttc aga tcc atg atc agt gac cca aac       1632
Lys Ile Ile Thr Val Gly Glu Phe Arg Ser Met Ile Ser Asp Pro Asn           
    530                 535                 540                           

ttt gcg acc aat gac aaa att cgg atg ata gag att atg cta cca cca       1680
Phe Ala Thr Asn Asp Lys Ile Arg Met Ile Glu Ile Met Leu Pro Pro           
545                 550                 555                 560           

agg gat gtt cca cag gct ctg ctt gac agg tgg gtg gta gaa aaa gaa       1728
Arg Asp Val Pro Gln Ala Leu Leu Asp Arg Trp Val Val Glu Lys Glu           
                565                 570                 575               

cag agc aaa caa gtg caa gag gag aac gaa aat tct agc gca gta aat       1776
Gln Ser Lys Gln Val Gln Glu Glu Asn Glu Asn Ser Ser Ala Val Asn           
            580                 585                 590                   

acg cca act cca gaa ttc caa cca ctt cta aaa aaa aat caa gtt gga       1824
Thr Pro Thr Pro Glu Phe Gln Pro Leu Leu Lys Lys Asn Gln Val Gly           
        595                 600                 605                       

tac tga                                                               1830
Tyr                                                                       
                                                                          


<210>  25
<211>  609
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  25

Met Asn Ser Ser Tyr Thr Gln Arg Tyr Ala Leu Pro Lys Cys Ile Ala 
1               5                   10                  15      


Ile Ser Asp Tyr Leu Phe His Arg Leu Asn Gln Leu Asn Ile His Thr 
            20                  25                  30          


Ile Phe Gly Leu Ser Gly Glu Phe Ser Met Pro Leu Leu Asp Lys Leu 
        35                  40                  45              


Tyr Asn Ile Pro Asn Leu Arg Trp Ala Gly Asn Ser Asn Glu Leu Asn 
    50                  55                  60                  


Ala Ala Tyr Ala Ala Asp Gly Tyr Ser Arg Leu Lys Gly Leu Gly Cys 
65                  70                  75                  80  


Leu Ile Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile Asn Gly Val 
                85                  90                  95      


Ala Gly Ser Tyr Ala Glu His Val Gly Ile Leu His Ile Val Gly Met 
            100                 105                 110         


Pro Pro Thr Ser Ala Gln Thr Lys Gln Leu Leu Leu His His Thr Leu 
        115                 120                 125             


Gly Asn Gly Asp Phe Thr Val Phe His Arg Ile Ala Ser Asp Val Ala 
    130                 135                 140                 


Cys Tyr Thr Thr Leu Ile Ile Asp Ser Glu Leu Cys Ala Asp Glu Val 
145                 150                 155                 160 


Asp Lys Cys Ile Lys Lys Ala Trp Ile Glu Gln Arg Pro Val Tyr Met 
                165                 170                 175     


Gly Met Pro Val Asn Gln Val Asn Leu Pro Ile Glu Ser Ala Arg Leu 
            180                 185                 190         


Asn Thr Pro Leu Asp Leu Gln Leu His Lys Asn Asp Pro Asp Val Glu 
        195                 200                 205             


Lys Glu Val Ile Ser Arg Ile Leu Ser Phe Ile Tyr Lys Ser Gln Asn 
    210                 215                 220                 


Pro Ala Ile Ile Val Asp Ala Cys Thr Ser Arg Gln Asn Leu Ile Glu 
225                 230                 235                 240 


Glu Thr Lys Glu Leu Cys Asn Arg Leu Lys Phe Pro Val Phe Val Thr 
                245                 250                 255     


Pro Met Gly Lys Gly Thr Val Asn Glu Thr Asp Pro Gln Phe Gly Gly 
            260                 265                 270         


Val Phe Thr Gly Ser Ile Ser Ala Pro Glu Val Arg Glu Val Val Asp 
        275                 280                 285             


Phe Ala Asp Phe Ile Ile Val Ile Gly Cys Met Leu Ser Glu Phe Ser 
    290                 295                 300                 


Thr Ser Thr Phe His Phe Gln Tyr Lys Thr Lys Asn Cys Ala Leu Leu 
305                 310                 315                 320 


Tyr Ser Thr Ser Val Lys Leu Lys Asn Ala Thr Tyr Pro Asp Leu Ser 
                325                 330                 335     


Ile Lys Leu Leu Leu Gln Lys Ile Leu Ala Asn Leu Asp Glu Ser Lys 
            340                 345                 350         


Leu Ser Tyr Gln Pro Ser Glu Gln Pro Ser Met Met Val Pro Arg Pro 
        355                 360                 365             


Tyr Pro Ala Gly Asn Val Leu Leu Arg Gln Glu Trp Val Trp Asn Glu 
    370                 375                 380                 


Ile Ser His Trp Phe Gln Pro Gly Asp Ile Ile Ile Thr Glu Thr Gly 
385                 390                 395                 400 


Ala Ser Ala Phe Gly Val Asn Gln Thr Arg Phe Pro Val Asn Thr Leu 
                405                 410                 415     


Gly Ile Ser Gln Ala Leu Trp Gly Ser Val Gly Tyr Thr Met Gly Ala 
            420                 425                 430         


Cys Leu Gly Ala Glu Phe Ala Val Gln Glu Ile Asn Lys Asp Lys Phe 
        435                 440                 445             


Pro Ala Thr Lys His Arg Val Ile Leu Phe Met Gly Asp Gly Ala Phe 
    450                 455                 460                 


Gln Leu Thr Val Gln Glu Leu Ser Thr Ile Val Lys Trp Gly Leu Thr 
465                 470                 475                 480 


Pro Tyr Ile Phe Val Met Asn Asn Gln Gly Tyr Ser Val Asp Arg Phe 
                485                 490                 495     


Leu His His Arg Ser Asp Ala Ser Tyr Tyr Asp Ile Gln Pro Trp Asn 
            500                 505                 510         


Tyr Leu Gly Leu Leu Arg Val Phe Gly Cys Thr Asn Tyr Glu Thr Lys 
        515                 520                 525             


Lys Ile Ile Thr Val Gly Glu Phe Arg Ser Met Ile Ser Asp Pro Asn 
    530                 535                 540                 


Phe Ala Thr Asn Asp Lys Ile Arg Met Ile Glu Ile Met Leu Pro Pro 
545                 550                 555                 560 


Arg Asp Val Pro Gln Ala Leu Leu Asp Arg Trp Val Val Glu Lys Glu 
                565                 570                 575     


Gln Ser Lys Gln Val Gln Glu Glu Asn Glu Asn Ser Ser Ala Val Asn 
            580                 585                 590         


Thr Pro Thr Pro Glu Phe Gln Pro Leu Leu Lys Lys Asn Gln Val Gly 
        595                 600                 605             


Tyr 
    


<210>  26
<211>  1665
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  CDS
<222>  (1)..(1665)

<400>  26
ttg aag agt gaa tac aca att gga aga tat ttg tta gac cgt tta tca         48
Leu Lys Ser Glu Tyr Thr Ile Gly Arg Tyr Leu Leu Asp Arg Leu Ser           
1               5                   10                  15                

gag ttg ggt att cgg cat atc ttt ggt gta cct gga gat tac aat cta         96
Glu Leu Gly Ile Arg His Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu           
            20                  25                  30                    

tcc ttt tta gac tat ata atg gag tac aaa ggg ata gat tgg gtt gga        144
Ser Phe Leu Asp Tyr Ile Met Glu Tyr Lys Gly Ile Asp Trp Val Gly           
        35                  40                  45                        

aat tgc aat gaa ttg aat gct ggg tat gct gct gat gga tat gca aga        192
Asn Cys Asn Glu Leu Asn Ala Gly Tyr Ala Ala Asp Gly Tyr Ala Arg           
    50                  55                  60                            

ata aat gga att gga gcc ata ctt aca aca ttt ggt gtt gga gaa tta        240
Ile Asn Gly Ile Gly Ala Ile Leu Thr Thr Phe Gly Val Gly Glu Leu           
65                  70                  75                  80            

agt gcc att aac gca att gct ggg gca tac gct gag caa gtt cca gtt        288
Ser Ala Ile Asn Ala Ile Ala Gly Ala Tyr Ala Glu Gln Val Pro Val           
                85                  90                  95                

gtt aaa att aca ggt atc ccc aca gca aaa gtt agg gac aat gga tta        336
Val Lys Ile Thr Gly Ile Pro Thr Ala Lys Val Arg Asp Asn Gly Leu           
            100                 105                 110                   

tat gta cac cac aca tta ggt gac gga agg ttt gat cac ttt ttt gaa        384
Tyr Val His His Thr Leu Gly Asp Gly Arg Phe Asp His Phe Phe Glu           
        115                 120                 125                       

atg ttt aga gaa gta aca gtt gct gag gca tta cta agc gaa gaa aat        432
Met Phe Arg Glu Val Thr Val Ala Glu Ala Leu Leu Ser Glu Glu Asn           
    130                 135                 140                           

gca gca caa gaa att gat cgt gtt ctt att tca tgc tgg aga caa aaa        480
Ala Ala Gln Glu Ile Asp Arg Val Leu Ile Ser Cys Trp Arg Gln Lys           
145                 150                 155                 160           

cgt cct gtt ctt ata aat tta ccg att gat gta tat gat aaa cca att        528
Arg Pro Val Leu Ile Asn Leu Pro Ile Asp Val Tyr Asp Lys Pro Ile           
                165                 170                 175               

aac aaa cca tta aag cca tta ctc gat tat act att tca agt aac aaa        576
Asn Lys Pro Leu Lys Pro Leu Leu Asp Tyr Thr Ile Ser Ser Asn Lys           
            180                 185                 190                   

gag gct gca tgt gaa ttt gtt aca gaa ata gta cct ata ata aat agg        624
Glu Ala Ala Cys Glu Phe Val Thr Glu Ile Val Pro Ile Ile Asn Arg           
        195                 200                 205                       

gca aaa aag cct gtt att ctt gca gat tat gga gta tat cgt tac caa        672
Ala Lys Lys Pro Val Ile Leu Ala Asp Tyr Gly Val Tyr Arg Tyr Gln           
    210                 215                 220                           

gtt caa cat gtg ctt aaa aac ttg gcc gaa aaa acc gga ttt cct gtg        720
Val Gln His Val Leu Lys Asn Leu Ala Glu Lys Thr Gly Phe Pro Val           
225                 230                 235                 240           

gct aca cta agt atg gga aaa ggt gtt ttc aat gaa gca cac cct caa        768
Ala Thr Leu Ser Met Gly Lys Gly Val Phe Asn Glu Ala His Pro Gln           
                245                 250                 255               

ttt att ggt gtt tat aat ggt gat gta agt tct cct tat tta agg cag        816
Phe Ile Gly Val Tyr Asn Gly Asp Val Ser Ser Pro Tyr Leu Arg Gln           
            260                 265                 270                   

cga gtt gat gaa gca gac tgc att att agc gtt ggt gta aaa ttg acg        864
Arg Val Asp Glu Ala Asp Cys Ile Ile Ser Val Gly Val Lys Leu Thr           
        275                 280                 285                       

gat tca acc aca ggg gga ttt tct cat gga ttt tct aaa agg aat gta        912
Asp Ser Thr Thr Gly Gly Phe Ser His Gly Phe Ser Lys Arg Asn Val           
    290                 295                 300                           

att cac att gat cct ttt tca ata aag gca aaa ggt aaa aaa tat gca        960
Ile His Ile Asp Pro Phe Ser Ile Lys Ala Lys Gly Lys Lys Tyr Ala           
305                 310                 315                 320           

cct att acg atg aaa gat gct tta aca gaa tta aca agt aaa att gag       1008
Pro Ile Thr Met Lys Asp Ala Leu Thr Glu Leu Thr Ser Lys Ile Glu           
                325                 330                 335               

cat aga aac ttt gag gat tta gat ata aag cct tac aaa tca gat aat       1056
His Arg Asn Phe Glu Asp Leu Asp Ile Lys Pro Tyr Lys Ser Asp Asn           
            340                 345                 350                   

caa aag tat ttt gca aaa gag aag cca att aca caa aaa cgt ttt ttt       1104
Gln Lys Tyr Phe Ala Lys Glu Lys Pro Ile Thr Gln Lys Arg Phe Phe           
        355                 360                 365                       

gag cgt att gct cac ttt ata aaa gaa aaa gat gta tta tta gca gaa       1152
Glu Arg Ile Ala His Phe Ile Lys Glu Lys Asp Val Leu Leu Ala Glu           
    370                 375                 380                           

cag ggt aca tgc ttt ttt ggt gcg tca acc ata caa cta ccc aaa gat       1200
Gln Gly Thr Cys Phe Phe Gly Ala Ser Thr Ile Gln Leu Pro Lys Asp           
385                 390                 395                 400           

gca act ttt att ggt caa cct tta tgg gga tct att gga tac aca ctt       1248
Ala Thr Phe Ile Gly Gln Pro Leu Trp Gly Ser Ile Gly Tyr Thr Leu           
                405                 410                 415               

cct gct tta tta ggt tca caa tta gct gat caa aaa agg cgt aat att       1296
Pro Ala Leu Leu Gly Ser Gln Leu Ala Asp Gln Lys Arg Arg Asn Ile           
            420                 425                 430                   

ctt tta att ggg gat ggt gca ttt caa atg aca gca caa gaa att tca       1344
Leu Leu Ile Gly Asp Gly Ala Phe Gln Met Thr Ala Gln Glu Ile Ser           
        435                 440                 445                       

aca atg ctt cgt tta caa atc aaa cct att att ttt tta att aat aac       1392
Thr Met Leu Arg Leu Gln Ile Lys Pro Ile Ile Phe Leu Ile Asn Asn           
    450                 455                 460                           

gat ggt tat aca att gaa cgt gct att cat ggt aga gaa caa gta tat       1440
Asp Gly Tyr Thr Ile Glu Arg Ala Ile His Gly Arg Glu Gln Val Tyr           
465                 470                 475                 480           

aac aat att caa atg tgg cga tat cat aat gtt cca aag gtt tta ggt       1488
Asn Asn Ile Gln Met Trp Arg Tyr His Asn Val Pro Lys Val Leu Gly           
                485                 490                 495               

cct aaa gaa tgc agc tta acc ttt aaa gta caa agt gaa act gaa ctt       1536
Pro Lys Glu Cys Ser Leu Thr Phe Lys Val Gln Ser Glu Thr Glu Leu           
            500                 505                 510                   

gaa aag gct ctt tta gtg gca gat aag gat tgt gaa cat ttg att ttt       1584
Glu Lys Ala Leu Leu Val Ala Asp Lys Asp Cys Glu His Leu Ile Phe           
        515                 520                 525                       

ata gaa gtt gtt atg gat cgt tat gat aaa ccc gag cct tta gaa cgt       1632
Ile Glu Val Val Met Asp Arg Tyr Asp Lys Pro Glu Pro Leu Glu Arg           
    530                 535                 540                           

ctt tcg aaa cgt ttt gca aat caa aat aat tag                           1665
Leu Ser Lys Arg Phe Ala Asn Gln Asn Asn                                   
545                 550                                                   


<210>  27
<211>  554
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  27

Leu Lys Ser Glu Tyr Thr Ile Gly Arg Tyr Leu Leu Asp Arg Leu Ser 
1               5                   10                  15      


Glu Leu Gly Ile Arg His Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu 
            20                  25                  30          


Ser Phe Leu Asp Tyr Ile Met Glu Tyr Lys Gly Ile Asp Trp Val Gly 
        35                  40                  45              


Asn Cys Asn Glu Leu Asn Ala Gly Tyr Ala Ala Asp Gly Tyr Ala Arg 
    50                  55                  60                  


Ile Asn Gly Ile Gly Ala Ile Leu Thr Thr Phe Gly Val Gly Glu Leu 
65                  70                  75                  80  


Ser Ala Ile Asn Ala Ile Ala Gly Ala Tyr Ala Glu Gln Val Pro Val 
                85                  90                  95      


Val Lys Ile Thr Gly Ile Pro Thr Ala Lys Val Arg Asp Asn Gly Leu 
            100                 105                 110         


Tyr Val His His Thr Leu Gly Asp Gly Arg Phe Asp His Phe Phe Glu 
        115                 120                 125             


Met Phe Arg Glu Val Thr Val Ala Glu Ala Leu Leu Ser Glu Glu Asn 
    130                 135                 140                 


Ala Ala Gln Glu Ile Asp Arg Val Leu Ile Ser Cys Trp Arg Gln Lys 
145                 150                 155                 160 


Arg Pro Val Leu Ile Asn Leu Pro Ile Asp Val Tyr Asp Lys Pro Ile 
                165                 170                 175     


Asn Lys Pro Leu Lys Pro Leu Leu Asp Tyr Thr Ile Ser Ser Asn Lys 
            180                 185                 190         


Glu Ala Ala Cys Glu Phe Val Thr Glu Ile Val Pro Ile Ile Asn Arg 
        195                 200                 205             


Ala Lys Lys Pro Val Ile Leu Ala Asp Tyr Gly Val Tyr Arg Tyr Gln 
    210                 215                 220                 


Val Gln His Val Leu Lys Asn Leu Ala Glu Lys Thr Gly Phe Pro Val 
225                 230                 235                 240 


Ala Thr Leu Ser Met Gly Lys Gly Val Phe Asn Glu Ala His Pro Gln 
                245                 250                 255     


Phe Ile Gly Val Tyr Asn Gly Asp Val Ser Ser Pro Tyr Leu Arg Gln 
            260                 265                 270         


Arg Val Asp Glu Ala Asp Cys Ile Ile Ser Val Gly Val Lys Leu Thr 
        275                 280                 285             


Asp Ser Thr Thr Gly Gly Phe Ser His Gly Phe Ser Lys Arg Asn Val 
    290                 295                 300                 


Ile His Ile Asp Pro Phe Ser Ile Lys Ala Lys Gly Lys Lys Tyr Ala 
305                 310                 315                 320 


Pro Ile Thr Met Lys Asp Ala Leu Thr Glu Leu Thr Ser Lys Ile Glu 
                325                 330                 335     


His Arg Asn Phe Glu Asp Leu Asp Ile Lys Pro Tyr Lys Ser Asp Asn 
            340                 345                 350         


Gln Lys Tyr Phe Ala Lys Glu Lys Pro Ile Thr Gln Lys Arg Phe Phe 
        355                 360                 365             


Glu Arg Ile Ala His Phe Ile Lys Glu Lys Asp Val Leu Leu Ala Glu 
    370                 375                 380                 


Gln Gly Thr Cys Phe Phe Gly Ala Ser Thr Ile Gln Leu Pro Lys Asp 
385                 390                 395                 400 


Ala Thr Phe Ile Gly Gln Pro Leu Trp Gly Ser Ile Gly Tyr Thr Leu 
                405                 410                 415     


Pro Ala Leu Leu Gly Ser Gln Leu Ala Asp Gln Lys Arg Arg Asn Ile 
            420                 425                 430         


Leu Leu Ile Gly Asp Gly Ala Phe Gln Met Thr Ala Gln Glu Ile Ser 
        435                 440                 445             


Thr Met Leu Arg Leu Gln Ile Lys Pro Ile Ile Phe Leu Ile Asn Asn 
    450                 455                 460                 


Asp Gly Tyr Thr Ile Glu Arg Ala Ile His Gly Arg Glu Gln Val Tyr 
465                 470                 475                 480 


Asn Asn Ile Gln Met Trp Arg Tyr His Asn Val Pro Lys Val Leu Gly 
                485                 490                 495     


Pro Lys Glu Cys Ser Leu Thr Phe Lys Val Gln Ser Glu Thr Glu Leu 
            500                 505                 510         


Glu Lys Ala Leu Leu Val Ala Asp Lys Asp Cys Glu His Leu Ile Phe 
        515                 520                 525             


Ile Glu Val Val Met Asp Arg Tyr Asp Lys Pro Glu Pro Leu Glu Arg 
    530                 535                 540                 


Leu Ser Lys Arg Phe Ala Asn Gln Asn Asn 
545                 550                 


<210>  28
<211>  1056
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1056)

<400>  28
atg cct tcg caa gtc att cct gaa aaa caa aag gct att gtc ttt tat         48
Met Pro Ser Gln Val Ile Pro Glu Lys Gln Lys Ala Ile Val Phe Tyr           
1               5                   10                  15                

gag aca gat gga aaa ttg gaa tat aaa gac gtc aca gtt ccg gaa cct         96
Glu Thr Asp Gly Lys Leu Glu Tyr Lys Asp Val Thr Val Pro Glu Pro           
            20                  25                  30                    

aag cct aac gaa att tta gtc cac gtt aaa tat tct ggt gtt tgt cat        144
Lys Pro Asn Glu Ile Leu Val His Val Lys Tyr Ser Gly Val Cys His           
        35                  40                  45                        

agt gac ttg cac gcg tgg cac ggt gat tgg cca ttt caa ttg aaa ttt        192
Ser Asp Leu His Ala Trp His Gly Asp Trp Pro Phe Gln Leu Lys Phe           
    50                  55                  60                            

cca tta atc ggt ggt cac gaa ggt gct ggt gtt gtt gtt aag ttg gga        240
Pro Leu Ile Gly Gly His Glu Gly Ala Gly Val Val Val Lys Leu Gly           
65                  70                  75                  80            

tct aac gtt aag ggc tgg aaa gtc ggt gat ttt gca ggt ata aaa tgg        288
Ser Asn Val Lys Gly Trp Lys Val Gly Asp Phe Ala Gly Ile Lys Trp           
                85                  90                  95                

ttg aat ggg act tgc atg tcc tgt gaa tat tgt gaa gta ggt aat gaa        336
Leu Asn Gly Thr Cys Met Ser Cys Glu Tyr Cys Glu Val Gly Asn Glu           
            100                 105                 110                   

tct caa tgt cct tat ttg gat ggt act ggc ttc aca cat gat ggt act        384
Ser Gln Cys Pro Tyr Leu Asp Gly Thr Gly Phe Thr His Asp Gly Thr           
        115                 120                 125                       

ttt caa gaa tac gca act gcc gat gcc gtt caa gct gcc cat att cca        432
Phe Gln Glu Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro           
    130                 135                 140                           

cca aac gtc aat ctt gct gaa gtt gcc cca atc ttg tgt gca ggt atc        480
Pro Asn Val Asn Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile           
145                 150                 155                 160           

act gtt tat aag gcg ttg aaa aga gcc aat gtg ata cca ggc caa tgg        528
Thr Val Tyr Lys Ala Leu Lys Arg Ala Asn Val Ile Pro Gly Gln Trp           
                165                 170                 175               

gtc act ata tcc ggt gca tgc ggt ggc ttg ggt tct ctg gca atc caa        576
Val Thr Ile Ser Gly Ala Cys Gly Gly Leu Gly Ser Leu Ala Ile Gln           
            180                 185                 190                   

tac gcc ctt gct atg ggt tac agg gtc att ggt atc gat ggt ggt aat        624
Tyr Ala Leu Ala Met Gly Tyr Arg Val Ile Gly Ile Asp Gly Gly Asn           
        195                 200                 205                       

gcc aag cga aag tta ttt gaa caa tta ggc gga gaa ata ttc atc gat        672
Ala Lys Arg Lys Leu Phe Glu Gln Leu Gly Gly Glu Ile Phe Ile Asp           
    210                 215                 220                           

ttc acg gaa gaa aaa gac att gtt ggt gct ata ata aag gcc act aat        720
Phe Thr Glu Glu Lys Asp Ile Val Gly Ala Ile Ile Lys Ala Thr Asn           
225                 230                 235                 240           

ggc ggt tct cat gga gtt att aat gtg tct gtt tct gaa gca gct atc        768
Gly Gly Ser His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile           
                245                 250                 255               

gag gct tct acg agg tat tgt agg ccc aat ggt act gtc gtc ctg gtt        816
Glu Ala Ser Thr Arg Tyr Cys Arg Pro Asn Gly Thr Val Val Leu Val           
            260                 265                 270                   

ggt atg cca gct cat gct tac tgc aat tcc gat gtt ttc aat caa gtt        864
Gly Met Pro Ala His Ala Tyr Cys Asn Ser Asp Val Phe Asn Gln Val           
        275                 280                 285                       

gta aaa tca atc tcc atc gtt gga tct tgt gtt gga aat aga gct gat        912
Val Lys Ser Ile Ser Ile Val Gly Ser Cys Val Gly Asn Arg Ala Asp           
    290                 295                 300                           

aca agg gag gct tta gat ttc ttc gcc aga ggt ttg atc aaa tct ccg        960
Thr Arg Glu Ala Leu Asp Phe Phe Ala Arg Gly Leu Ile Lys Ser Pro           
305                 310                 315                 320           

atc cac tta gct ggc cta tcg gat gtt cct gaa att ttt gca aag atg       1008
Ile His Leu Ala Gly Leu Ser Asp Val Pro Glu Ile Phe Ala Lys Met           
                325                 330                 335               

gag aag ggt gaa att gtt ggt aga tat gtt gtt gag act tct aaa tga       1056
Glu Lys Gly Glu Ile Val Gly Arg Tyr Val Val Glu Thr Ser Lys               
            340                 345                 350                   


<210>  29
<211>  351
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  29

Met Pro Ser Gln Val Ile Pro Glu Lys Gln Lys Ala Ile Val Phe Tyr 
1               5                   10                  15      


Glu Thr Asp Gly Lys Leu Glu Tyr Lys Asp Val Thr Val Pro Glu Pro 
            20                  25                  30          


Lys Pro Asn Glu Ile Leu Val His Val Lys Tyr Ser Gly Val Cys His 
        35                  40                  45              


Ser Asp Leu His Ala Trp His Gly Asp Trp Pro Phe Gln Leu Lys Phe 
    50                  55                  60                  


Pro Leu Ile Gly Gly His Glu Gly Ala Gly Val Val Val Lys Leu Gly 
65                  70                  75                  80  


Ser Asn Val Lys Gly Trp Lys Val Gly Asp Phe Ala Gly Ile Lys Trp 
                85                  90                  95      


Leu Asn Gly Thr Cys Met Ser Cys Glu Tyr Cys Glu Val Gly Asn Glu 
            100                 105                 110         


Ser Gln Cys Pro Tyr Leu Asp Gly Thr Gly Phe Thr His Asp Gly Thr 
        115                 120                 125             


Phe Gln Glu Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro 
    130                 135                 140                 


Pro Asn Val Asn Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile 
145                 150                 155                 160 


Thr Val Tyr Lys Ala Leu Lys Arg Ala Asn Val Ile Pro Gly Gln Trp 
                165                 170                 175     


Val Thr Ile Ser Gly Ala Cys Gly Gly Leu Gly Ser Leu Ala Ile Gln 
            180                 185                 190         


Tyr Ala Leu Ala Met Gly Tyr Arg Val Ile Gly Ile Asp Gly Gly Asn 
        195                 200                 205             


Ala Lys Arg Lys Leu Phe Glu Gln Leu Gly Gly Glu Ile Phe Ile Asp 
    210                 215                 220                 


Phe Thr Glu Glu Lys Asp Ile Val Gly Ala Ile Ile Lys Ala Thr Asn 
225                 230                 235                 240 


Gly Gly Ser His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile 
                245                 250                 255     


Glu Ala Ser Thr Arg Tyr Cys Arg Pro Asn Gly Thr Val Val Leu Val 
            260                 265                 270         


Gly Met Pro Ala His Ala Tyr Cys Asn Ser Asp Val Phe Asn Gln Val 
        275                 280                 285             


Val Lys Ser Ile Ser Ile Val Gly Ser Cys Val Gly Asn Arg Ala Asp 
    290                 295                 300                 


Thr Arg Glu Ala Leu Asp Phe Phe Ala Arg Gly Leu Ile Lys Ser Pro 
305                 310                 315                 320 


Ile His Leu Ala Gly Leu Ser Asp Val Pro Glu Ile Phe Ala Lys Met 
                325                 330                 335     


Glu Lys Gly Glu Ile Val Gly Arg Tyr Val Val Glu Thr Ser Lys 
            340                 345                 350     


<210>  30
<211>  1725
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1725)

<400>  30
atg gag atg ttg tct gga gcc gag atg gtc gtc cga tcg ctt atc gat         48
Met Glu Met Leu Ser Gly Ala Glu Met Val Val Arg Ser Leu Ile Asp           
1               5                   10                  15                

cag ggc gtt aaa caa gta ttc ggt tat ccc gga ggc gca gtc ctt gat         96
Gln Gly Val Lys Gln Val Phe Gly Tyr Pro Gly Gly Ala Val Leu Asp           
            20                  25                  30                    

att tat gat gca ttg cat acc gtg ggt ggt att gat cat gta tta gtt        144
Ile Tyr Asp Ala Leu His Thr Val Gly Gly Ile Asp His Val Leu Val           
        35                  40                  45                        

cgt cat gag cag gcg gcg gtg cat atg gcc gat ggc ctg gcg cgc gcg        192
Arg His Glu Gln Ala Ala Val His Met Ala Asp Gly Leu Ala Arg Ala           
    50                  55                  60                            

acc ggg gaa gtc ggc gtc gtg ctg gta acg tcg ggt cca ggg gcg acc        240
Thr Gly Glu Val Gly Val Val Leu Val Thr Ser Gly Pro Gly Ala Thr           
65                  70                  75                  80            

aat gcg att act ggc atc gcc acc gct tat atg gat tcc att cca tta        288
Asn Ala Ile Thr Gly Ile Ala Thr Ala Tyr Met Asp Ser Ile Pro Leu           
                85                  90                  95                

gtt gtc ctt tcc ggg cag gta gcg acc tcg ttg ata ggt tac gat gcc        336
Val Val Leu Ser Gly Gln Val Ala Thr Ser Leu Ile Gly Tyr Asp Ala           
            100                 105                 110                   

ttt cag gag tgc gac atg gtg ggg att tcg cga ccg gtg gtt aaa cac        384
Phe Gln Glu Cys Asp Met Val Gly Ile Ser Arg Pro Val Val Lys His           
        115                 120                 125                       

agt ttt ctg gtt aag caa acg gaa gac att ccg cag gtg ctg aaa aag        432
Ser Phe Leu Val Lys Gln Thr Glu Asp Ile Pro Gln Val Leu Lys Lys           
    130                 135                 140                           

gct ttc tgg ctg gcg gca agt ggt cgc cca gga cca gta gtc gtt gat        480
Ala Phe Trp Leu Ala Ala Ser Gly Arg Pro Gly Pro Val Val Val Asp           
145                 150                 155                 160           

tta ccg aaa gat att ctt aat ccg gcg aac aaa tta ccc tat gtc tgg        528
Leu Pro Lys Asp Ile Leu Asn Pro Ala Asn Lys Leu Pro Tyr Val Trp           
                165                 170                 175               

ccg gag tcg gtc agt atg cgt tct tac aat ccc act act acc gga cat        576
Pro Glu Ser Val Ser Met Arg Ser Tyr Asn Pro Thr Thr Thr Gly His           
            180                 185                 190                   

aaa ggg caa att aag cgt gct ctg caa acg ctg gta gcg gca aaa aaa        624
Lys Gly Gln Ile Lys Arg Ala Leu Gln Thr Leu Val Ala Ala Lys Lys           
        195                 200                 205                       

ccg gtt gtc tac gta ggc ggt ggg gca atc acg gcg ggc tgc cat cag        672
Pro Val Val Tyr Val Gly Gly Gly Ala Ile Thr Ala Gly Cys His Gln           
    210                 215                 220                           

cag ttg aaa gaa acg gtg gag gcg ttg aat ctg ccc gtt gtt tgc tca        720
Gln Leu Lys Glu Thr Val Glu Ala Leu Asn Leu Pro Val Val Cys Ser           
225                 230                 235                 240           

ttg atg ggg ctg ggg gcg ttt ccg gca acg cat cgt cag gca ctg ggc        768
Leu Met Gly Leu Gly Ala Phe Pro Ala Thr His Arg Gln Ala Leu Gly           
                245                 250                 255               

atg ctg gga atg cac ggt acc tac gaa gcc aat atg acg atg cat aac        816
Met Leu Gly Met His Gly Thr Tyr Glu Ala Asn Met Thr Met His Asn           
            260                 265                 270                   

gcg gat gtg att ttc gcc gtc ggg gta cga ttt gat gac cga acg acg        864
Ala Asp Val Ile Phe Ala Val Gly Val Arg Phe Asp Asp Arg Thr Thr           
        275                 280                 285                       

aac aat ctg gca aag tac tgc cca aat gcc act gtt ctg cat atc gat        912
Asn Asn Leu Ala Lys Tyr Cys Pro Asn Ala Thr Val Leu His Ile Asp           
    290                 295                 300                           

att gat cct act tcc att tct aaa acc gtg act gcg gat atc ccg att        960
Ile Asp Pro Thr Ser Ile Ser Lys Thr Val Thr Ala Asp Ile Pro Ile           
305                 310                 315                 320           

gtg ggg gat gct cgc cag gtc ctc gaa caa atg ctt gaa ctc ttg tcg       1008
Val Gly Asp Ala Arg Gln Val Leu Glu Gln Met Leu Glu Leu Leu Ser           
                325                 330                 335               

caa gaa tcc gcc cat caa cca ctg gat gag atc cgc gac tgg tgg cag       1056
Gln Glu Ser Ala His Gln Pro Leu Asp Glu Ile Arg Asp Trp Trp Gln           
            340                 345                 350                   

caa att gaa cag tgg cgc gct cgt cag tgc ctg aaa tat gac act cac       1104
Gln Ile Glu Gln Trp Arg Ala Arg Gln Cys Leu Lys Tyr Asp Thr His           
        355                 360                 365                       

agt gaa aag att aaa ccg cag gcg gtg atc gag act ctt tgg cgg ttg       1152
Ser Glu Lys Ile Lys Pro Gln Ala Val Ile Glu Thr Leu Trp Arg Leu           
    370                 375                 380                           

acg aag gga gac gct tac gtg acg tcc gat gtc ggg cag cac cag atg       1200
Thr Lys Gly Asp Ala Tyr Val Thr Ser Asp Val Gly Gln His Gln Met           
385                 390                 395                 400           

ttt gct gca ctt tat tat cca ttc gac aaa ccg cgt cgc tgg atc aat       1248
Phe Ala Ala Leu Tyr Tyr Pro Phe Asp Lys Pro Arg Arg Trp Ile Asn           
                405                 410                 415               

tcc ggt ggc ctc ggc acg atg ggt ttt ggt tta cct gcg gca ctg ggc       1296
Ser Gly Gly Leu Gly Thr Met Gly Phe Gly Leu Pro Ala Ala Leu Gly           
            420                 425                 430                   

gtc aaa atg gcg ttg cca gaa gaa acc gtg gtt tgc gtc act ggc gac       1344
Val Lys Met Ala Leu Pro Glu Glu Thr Val Val Cys Val Thr Gly Asp           
        435                 440                 445                       

ggc agt att cag atg aac atc cag gaa ctg tct acc gcg ttg caa tac       1392
Gly Ser Ile Gln Met Asn Ile Gln Glu Leu Ser Thr Ala Leu Gln Tyr           
    450                 455                 460                           

gag ttg ccc gta ctg gtg gtg aat ctc aat aac cgc tat ctg ggg atg       1440
Glu Leu Pro Val Leu Val Val Asn Leu Asn Asn Arg Tyr Leu Gly Met           
465                 470                 475                 480           

gtg aag cag tgg cag gac atg atc tat tcc ggc cgt cat tca caa tct       1488
Val Lys Gln Trp Gln Asp Met Ile Tyr Ser Gly Arg His Ser Gln Ser           
                485                 490                 495               

tat atg caa tcg cta ccc gat ttc gtc cgt ctg gcg gaa gcc tat ggg       1536
Tyr Met Gln Ser Leu Pro Asp Phe Val Arg Leu Ala Glu Ala Tyr Gly           
            500                 505                 510                   

cat gtc ggg atc cag att tct cat ccg cat gag ctg gaa agc aaa ctt       1584
His Val Gly Ile Gln Ile Ser His Pro His Glu Leu Glu Ser Lys Leu           
        515                 520                 525                       

agc gag gcg ctg gaa cag gtg cgc aat aat cgc ctg gtg ttt gtt gat       1632
Ser Glu Ala Leu Glu Gln Val Arg Asn Asn Arg Leu Val Phe Val Asp           
    530                 535                 540                           

gtt acc gtc gat ggc agc gag cac gtc tac ccg atg cag att cgc ggg       1680
Val Thr Val Asp Gly Ser Glu His Val Tyr Pro Met Gln Ile Arg Gly           
545                 550                 555                 560           

ggc gga atg gat gaa atg tgg tta agc aaa acg gag aga acc tga           1725
Gly Gly Met Asp Glu Met Trp Leu Ser Lys Thr Glu Arg Thr                   
                565                 570                                   


<210>  31
<211>  574
<212>  PRT
<213>  Escherichia coli

<400>  31

Met Glu Met Leu Ser Gly Ala Glu Met Val Val Arg Ser Leu Ile Asp 
1               5                   10                  15      


Gln Gly Val Lys Gln Val Phe Gly Tyr Pro Gly Gly Ala Val Leu Asp 
            20                  25                  30          


Ile Tyr Asp Ala Leu His Thr Val Gly Gly Ile Asp His Val Leu Val 
        35                  40                  45              


Arg His Glu Gln Ala Ala Val His Met Ala Asp Gly Leu Ala Arg Ala 
    50                  55                  60                  


Thr Gly Glu Val Gly Val Val Leu Val Thr Ser Gly Pro Gly Ala Thr 
65                  70                  75                  80  


Asn Ala Ile Thr Gly Ile Ala Thr Ala Tyr Met Asp Ser Ile Pro Leu 
                85                  90                  95      


Val Val Leu Ser Gly Gln Val Ala Thr Ser Leu Ile Gly Tyr Asp Ala 
            100                 105                 110         


Phe Gln Glu Cys Asp Met Val Gly Ile Ser Arg Pro Val Val Lys His 
        115                 120                 125             


Ser Phe Leu Val Lys Gln Thr Glu Asp Ile Pro Gln Val Leu Lys Lys 
    130                 135                 140                 


Ala Phe Trp Leu Ala Ala Ser Gly Arg Pro Gly Pro Val Val Val Asp 
145                 150                 155                 160 


Leu Pro Lys Asp Ile Leu Asn Pro Ala Asn Lys Leu Pro Tyr Val Trp 
                165                 170                 175     


Pro Glu Ser Val Ser Met Arg Ser Tyr Asn Pro Thr Thr Thr Gly His 
            180                 185                 190         


Lys Gly Gln Ile Lys Arg Ala Leu Gln Thr Leu Val Ala Ala Lys Lys 
        195                 200                 205             


Pro Val Val Tyr Val Gly Gly Gly Ala Ile Thr Ala Gly Cys His Gln 
    210                 215                 220                 


Gln Leu Lys Glu Thr Val Glu Ala Leu Asn Leu Pro Val Val Cys Ser 
225                 230                 235                 240 


Leu Met Gly Leu Gly Ala Phe Pro Ala Thr His Arg Gln Ala Leu Gly 
                245                 250                 255     


Met Leu Gly Met His Gly Thr Tyr Glu Ala Asn Met Thr Met His Asn 
            260                 265                 270         


Ala Asp Val Ile Phe Ala Val Gly Val Arg Phe Asp Asp Arg Thr Thr 
        275                 280                 285             


Asn Asn Leu Ala Lys Tyr Cys Pro Asn Ala Thr Val Leu His Ile Asp 
    290                 295                 300                 


Ile Asp Pro Thr Ser Ile Ser Lys Thr Val Thr Ala Asp Ile Pro Ile 
305                 310                 315                 320 


Val Gly Asp Ala Arg Gln Val Leu Glu Gln Met Leu Glu Leu Leu Ser 
                325                 330                 335     


Gln Glu Ser Ala His Gln Pro Leu Asp Glu Ile Arg Asp Trp Trp Gln 
            340                 345                 350         


Gln Ile Glu Gln Trp Arg Ala Arg Gln Cys Leu Lys Tyr Asp Thr His 
        355                 360                 365             


Ser Glu Lys Ile Lys Pro Gln Ala Val Ile Glu Thr Leu Trp Arg Leu 
    370                 375                 380                 


Thr Lys Gly Asp Ala Tyr Val Thr Ser Asp Val Gly Gln His Gln Met 
385                 390                 395                 400 


Phe Ala Ala Leu Tyr Tyr Pro Phe Asp Lys Pro Arg Arg Trp Ile Asn 
                405                 410                 415     


Ser Gly Gly Leu Gly Thr Met Gly Phe Gly Leu Pro Ala Ala Leu Gly 
            420                 425                 430         


Val Lys Met Ala Leu Pro Glu Glu Thr Val Val Cys Val Thr Gly Asp 
        435                 440                 445             


Gly Ser Ile Gln Met Asn Ile Gln Glu Leu Ser Thr Ala Leu Gln Tyr 
    450                 455                 460                 


Glu Leu Pro Val Leu Val Val Asn Leu Asn Asn Arg Tyr Leu Gly Met 
465                 470                 475                 480 


Val Lys Gln Trp Gln Asp Met Ile Tyr Ser Gly Arg His Ser Gln Ser 
                485                 490                 495     


Tyr Met Gln Ser Leu Pro Asp Phe Val Arg Leu Ala Glu Ala Tyr Gly 
            500                 505                 510         


His Val Gly Ile Gln Ile Ser His Pro His Glu Leu Glu Ser Lys Leu 
        515                 520                 525             


Ser Glu Ala Leu Glu Gln Val Arg Asn Asn Arg Leu Val Phe Val Asp 
    530                 535                 540                 


Val Thr Val Asp Gly Ser Glu His Val Tyr Pro Met Gln Ile Arg Gly 
545                 550                 555                 560 


Gly Gly Met Asp Glu Met Trp Leu Ser Lys Thr Glu Arg Thr 
                565                 570                 


<210>  32
<211>  492
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(492)

<400>  32
atg cgc cgg ata tta tca gtc tta ctc gaa aat gaa tca ggc gcg tta         48
Met Arg Arg Ile Leu Ser Val Leu Leu Glu Asn Glu Ser Gly Ala Leu           
1               5                   10                  15                

tcc cgc gtg att ggc ctt ttt tcc cag cgt ggc tac aac att gaa agc         96
Ser Arg Val Ile Gly Leu Phe Ser Gln Arg Gly Tyr Asn Ile Glu Ser           
            20                  25                  30                    

ctg acc gtt gcg cca acc gac gat ccg aca tta tcg cgt atg acc atc        144
Leu Thr Val Ala Pro Thr Asp Asp Pro Thr Leu Ser Arg Met Thr Ile           
        35                  40                  45                        

cag acc gtg ggc gat gaa aaa gta ctt gag cag atc gaa aag caa tta        192
Gln Thr Val Gly Asp Glu Lys Val Leu Glu Gln Ile Glu Lys Gln Leu           
    50                  55                  60                            

cac aaa ctg gtc gat gtc ttg cgc gtg agt gag ttg ggg cag ggc gcg        240
His Lys Leu Val Asp Val Leu Arg Val Ser Glu Leu Gly Gln Gly Ala           
65                  70                  75                  80            

cat gtt gag cgg gaa atc atg ctg gtg aaa att cag gcc agc ggt tac        288
His Val Glu Arg Glu Ile Met Leu Val Lys Ile Gln Ala Ser Gly Tyr           
                85                  90                  95                

ggg cgt gac gaa gtg aaa cgt aat acg gaa ata ttc cgt ggg caa att        336
Gly Arg Asp Glu Val Lys Arg Asn Thr Glu Ile Phe Arg Gly Gln Ile           
            100                 105                 110                   

atc gat gtc aca ccc tcg ctt tat acc gtt caa tta gca ggc acc agc        384
Ile Asp Val Thr Pro Ser Leu Tyr Thr Val Gln Leu Ala Gly Thr Ser           
        115                 120                 125                       

ggt aag ctt gat gca ttt tta gca tcg att cgc gat gtg gcg aaa att        432
Gly Lys Leu Asp Ala Phe Leu Ala Ser Ile Arg Asp Val Ala Lys Ile           
    130                 135                 140                           

gtg gag gtt gct cgc tct ggt gtg gtc gga ctt tcg cgc ggc gat aaa        480
Val Glu Val Ala Arg Ser Gly Val Val Gly Leu Ser Arg Gly Asp Lys           
145                 150                 155                 160           

ata atg cgt tga                                                        492
Ile Met Arg                                                               
                                                                          


<210>  33
<211>  163
<212>  PRT
<213>  Escherichia coli

<400>  33

Met Arg Arg Ile Leu Ser Val Leu Leu Glu Asn Glu Ser Gly Ala Leu 
1               5                   10                  15      


Ser Arg Val Ile Gly Leu Phe Ser Gln Arg Gly Tyr Asn Ile Glu Ser 
            20                  25                  30          


Leu Thr Val Ala Pro Thr Asp Asp Pro Thr Leu Ser Arg Met Thr Ile 
        35                  40                  45              


Gln Thr Val Gly Asp Glu Lys Val Leu Glu Gln Ile Glu Lys Gln Leu 
    50                  55                  60                  


His Lys Leu Val Asp Val Leu Arg Val Ser Glu Leu Gly Gln Gly Ala 
65                  70                  75                  80  


His Val Glu Arg Glu Ile Met Leu Val Lys Ile Gln Ala Ser Gly Tyr 
                85                  90                  95      


Gly Arg Asp Glu Val Lys Arg Asn Thr Glu Ile Phe Arg Gly Gln Ile 
            100                 105                 110         


Ile Asp Val Thr Pro Ser Leu Tyr Thr Val Gln Leu Ala Gly Thr Ser 
        115                 120                 125             


Gly Lys Leu Asp Ala Phe Leu Ala Ser Ile Arg Asp Val Ala Lys Ile 
    130                 135                 140                 


Val Glu Val Ala Arg Ser Gly Val Val Gly Leu Ser Arg Gly Asp Lys 
145                 150                 155                 160 


Ile Met Arg 
            


<210>  34
<211>  1476
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1476)

<400>  34
atg gct aac tac ttc aat aca ctg aat ctg cgc cag cag ctg gca cag         48
Met Ala Asn Tyr Phe Asn Thr Leu Asn Leu Arg Gln Gln Leu Ala Gln           
1               5                   10                  15                

ctg ggc aaa tgt cgc ttt atg ggc cgc gat gaa ttc gcc gat ggc gcg         96
Leu Gly Lys Cys Arg Phe Met Gly Arg Asp Glu Phe Ala Asp Gly Ala           
            20                  25                  30                    

agc tac ctt cag ggt aaa aaa gta gtc atc gtc ggc tgt ggc gca cag        144
Ser Tyr Leu Gln Gly Lys Lys Val Val Ile Val Gly Cys Gly Ala Gln           
        35                  40                  45                        

ggt ctg aac cag ggc ctg aac atg cgt gat tct ggt ctc gat atc tcc        192
Gly Leu Asn Gln Gly Leu Asn Met Arg Asp Ser Gly Leu Asp Ile Ser           
    50                  55                  60                            

tac gct ctg cgt aaa gaa gcg att gcc gag aag cgc gcg tcc tgg cgt        240
Tyr Ala Leu Arg Lys Glu Ala Ile Ala Glu Lys Arg Ala Ser Trp Arg           
65                  70                  75                  80            

aaa gcg acc gaa aat ggt ttt aaa gtg ggt act tac gaa gaa ctg atc        288
Lys Ala Thr Glu Asn Gly Phe Lys Val Gly Thr Tyr Glu Glu Leu Ile           
                85                  90                  95                

cca cag gcg gat ctg gtg att aac ctg acg ccg gac aag cag cac tct        336
Pro Gln Ala Asp Leu Val Ile Asn Leu Thr Pro Asp Lys Gln His Ser           
            100                 105                 110                   

gat gta gtg cgc acc gta cag cca ctg atg aaa gac ggc gcg gcg ctg        384
Asp Val Val Arg Thr Val Gln Pro Leu Met Lys Asp Gly Ala Ala Leu           
        115                 120                 125                       

ggc tac tcg cac ggt ttc aac atc gtc gaa gtg ggc gag cag atc cgt        432
Gly Tyr Ser His Gly Phe Asn Ile Val Glu Val Gly Glu Gln Ile Arg           
    130                 135                 140                           

aaa gat atc acc gta gtg atg gtt gcg ccg aaa tgc cca ggc acc gaa        480
Lys Asp Ile Thr Val Val Met Val Ala Pro Lys Cys Pro Gly Thr Glu           
145                 150                 155                 160           

gtg cgt gaa gag tac aaa cgt ggg ttc ggc gta ccg acg ctg att gcc        528
Val Arg Glu Glu Tyr Lys Arg Gly Phe Gly Val Pro Thr Leu Ile Ala           
                165                 170                 175               

gtt cac ccg gaa aac gat ccg aaa ggc gaa ggc atg gcg att gcc aaa        576
Val His Pro Glu Asn Asp Pro Lys Gly Glu Gly Met Ala Ile Ala Lys           
            180                 185                 190                   

gcc tgg gcg gct gca acc ggt ggt cac cgt gcg ggt gtg ctg gaa tcg        624
Ala Trp Ala Ala Ala Thr Gly Gly His Arg Ala Gly Val Leu Glu Ser           
        195                 200                 205                       

tcc ttc gtt gcg gaa gtg aaa tct gac ctg atg ggc gag caa acc atc        672
Ser Phe Val Ala Glu Val Lys Ser Asp Leu Met Gly Glu Gln Thr Ile           
    210                 215                 220                           

ctg tgc ggt atg ttg cag gct ggc tct ctg ctg tgc ttc gac aag ctg        720
Leu Cys Gly Met Leu Gln Ala Gly Ser Leu Leu Cys Phe Asp Lys Leu           
225                 230                 235                 240           

gtg gaa gaa ggt acc gat cca gca tac gca gaa aaa ctg att cag ttc        768
Val Glu Glu Gly Thr Asp Pro Ala Tyr Ala Glu Lys Leu Ile Gln Phe           
                245                 250                 255               

ggt tgg gaa acc atc acc gaa gca ctg aaa cag ggc ggc atc acc ctg        816
Gly Trp Glu Thr Ile Thr Glu Ala Leu Lys Gln Gly Gly Ile Thr Leu           
            260                 265                 270                   

atg atg gac cgt ctc tct aac ccg gcg aaa ctg cgt gct tat gcg ctt        864
Met Met Asp Arg Leu Ser Asn Pro Ala Lys Leu Arg Ala Tyr Ala Leu           
        275                 280                 285                       

tct gaa cag ctg aaa gag atc atg gca ccc ctg ttc cag aaa cat atg        912
Ser Glu Gln Leu Lys Glu Ile Met Ala Pro Leu Phe Gln Lys His Met           
    290                 295                 300                           

gac gac atc atc tcc ggc gaa ttc tct tcc ggt atg atg gcg gac tgg        960
Asp Asp Ile Ile Ser Gly Glu Phe Ser Ser Gly Met Met Ala Asp Trp           
305                 310                 315                 320           

gcc aac gat gat aag aaa ctg ctg acc tgg cgt gaa gag acc ggc aaa       1008
Ala Asn Asp Asp Lys Lys Leu Leu Thr Trp Arg Glu Glu Thr Gly Lys           
                325                 330                 335               

acc gcg ttt gaa acc gcg ccg cag tat gaa ggc aaa atc ggc gag cag       1056
Thr Ala Phe Glu Thr Ala Pro Gln Tyr Glu Gly Lys Ile Gly Glu Gln           
            340                 345                 350                   

gag tac ttc gat aaa ggc gta ctg atg att gcg atg gtg aaa gcg ggc       1104
Glu Tyr Phe Asp Lys Gly Val Leu Met Ile Ala Met Val Lys Ala Gly           
        355                 360                 365                       

gtt gaa ctg gcg ttc gaa acc atg gtc gat tcc ggc atc att gaa gag       1152
Val Glu Leu Ala Phe Glu Thr Met Val Asp Ser Gly Ile Ile Glu Glu           
    370                 375                 380                           

tct gca tat tat gaa tca ctg cac gag ctg ccg ctg att gcc aac acc       1200
Ser Ala Tyr Tyr Glu Ser Leu His Glu Leu Pro Leu Ile Ala Asn Thr           
385                 390                 395                 400           

atc gcc cgt aag cgt ctg tac gaa atg aac gtg gtt atc tct gat acc       1248
Ile Ala Arg Lys Arg Leu Tyr Glu Met Asn Val Val Ile Ser Asp Thr           
                405                 410                 415               

gct gag tac ggt aac tat ctg ttc tct tac gct tgt gtg ccg ttg ctg       1296
Ala Glu Tyr Gly Asn Tyr Leu Phe Ser Tyr Ala Cys Val Pro Leu Leu           
            420                 425                 430                   

aaa ccg ttt atg gca gag ctg caa ccg ggc gac ctg ggt aaa gct att       1344
Lys Pro Phe Met Ala Glu Leu Gln Pro Gly Asp Leu Gly Lys Ala Ile           
        435                 440                 445                       

ccg gaa ggc gcg gta gat aac ggg caa ctg cgt gat gtg aac gaa gcg       1392
Pro Glu Gly Ala Val Asp Asn Gly Gln Leu Arg Asp Val Asn Glu Ala           
    450                 455                 460                           

att cgc agc cat gcg att gag cag gta ggt aag aaa ctg cgc ggc tat       1440
Ile Arg Ser His Ala Ile Glu Gln Val Gly Lys Lys Leu Arg Gly Tyr           
465                 470                 475                 480           

atg aca gat atg aaa cgt att gct gtt gcg ggt taa                       1476
Met Thr Asp Met Lys Arg Ile Ala Val Ala Gly                               
                485                 490                                   


<210>  35
<211>  491
<212>  PRT
<213>  Escherichia coli

<400>  35

Met Ala Asn Tyr Phe Asn Thr Leu Asn Leu Arg Gln Gln Leu Ala Gln 
1               5                   10                  15      


Leu Gly Lys Cys Arg Phe Met Gly Arg Asp Glu Phe Ala Asp Gly Ala 
            20                  25                  30          


Ser Tyr Leu Gln Gly Lys Lys Val Val Ile Val Gly Cys Gly Ala Gln 
        35                  40                  45              


Gly Leu Asn Gln Gly Leu Asn Met Arg Asp Ser Gly Leu Asp Ile Ser 
    50                  55                  60                  


Tyr Ala Leu Arg Lys Glu Ala Ile Ala Glu Lys Arg Ala Ser Trp Arg 
65                  70                  75                  80  


Lys Ala Thr Glu Asn Gly Phe Lys Val Gly Thr Tyr Glu Glu Leu Ile 
                85                  90                  95      


Pro Gln Ala Asp Leu Val Ile Asn Leu Thr Pro Asp Lys Gln His Ser 
            100                 105                 110         


Asp Val Val Arg Thr Val Gln Pro Leu Met Lys Asp Gly Ala Ala Leu 
        115                 120                 125             


Gly Tyr Ser His Gly Phe Asn Ile Val Glu Val Gly Glu Gln Ile Arg 
    130                 135                 140                 


Lys Asp Ile Thr Val Val Met Val Ala Pro Lys Cys Pro Gly Thr Glu 
145                 150                 155                 160 


Val Arg Glu Glu Tyr Lys Arg Gly Phe Gly Val Pro Thr Leu Ile Ala 
                165                 170                 175     


Val His Pro Glu Asn Asp Pro Lys Gly Glu Gly Met Ala Ile Ala Lys 
            180                 185                 190         


Ala Trp Ala Ala Ala Thr Gly Gly His Arg Ala Gly Val Leu Glu Ser 
        195                 200                 205             


Ser Phe Val Ala Glu Val Lys Ser Asp Leu Met Gly Glu Gln Thr Ile 
    210                 215                 220                 


Leu Cys Gly Met Leu Gln Ala Gly Ser Leu Leu Cys Phe Asp Lys Leu 
225                 230                 235                 240 


Val Glu Glu Gly Thr Asp Pro Ala Tyr Ala Glu Lys Leu Ile Gln Phe 
                245                 250                 255     


Gly Trp Glu Thr Ile Thr Glu Ala Leu Lys Gln Gly Gly Ile Thr Leu 
            260                 265                 270         


Met Met Asp Arg Leu Ser Asn Pro Ala Lys Leu Arg Ala Tyr Ala Leu 
        275                 280                 285             


Ser Glu Gln Leu Lys Glu Ile Met Ala Pro Leu Phe Gln Lys His Met 
    290                 295                 300                 


Asp Asp Ile Ile Ser Gly Glu Phe Ser Ser Gly Met Met Ala Asp Trp 
305                 310                 315                 320 


Ala Asn Asp Asp Lys Lys Leu Leu Thr Trp Arg Glu Glu Thr Gly Lys 
                325                 330                 335     


Thr Ala Phe Glu Thr Ala Pro Gln Tyr Glu Gly Lys Ile Gly Glu Gln 
            340                 345                 350         


Glu Tyr Phe Asp Lys Gly Val Leu Met Ile Ala Met Val Lys Ala Gly 
        355                 360                 365             


Val Glu Leu Ala Phe Glu Thr Met Val Asp Ser Gly Ile Ile Glu Glu 
    370                 375                 380                 


Ser Ala Tyr Tyr Glu Ser Leu His Glu Leu Pro Leu Ile Ala Asn Thr 
385                 390                 395                 400 


Ile Ala Arg Lys Arg Leu Tyr Glu Met Asn Val Val Ile Ser Asp Thr 
                405                 410                 415     


Ala Glu Tyr Gly Asn Tyr Leu Phe Ser Tyr Ala Cys Val Pro Leu Leu 
            420                 425                 430         


Lys Pro Phe Met Ala Glu Leu Gln Pro Gly Asp Leu Gly Lys Ala Ile 
        435                 440                 445             


Pro Glu Gly Ala Val Asp Asn Gly Gln Leu Arg Asp Val Asn Glu Ala 
    450                 455                 460                 


Ile Arg Ser His Ala Ile Glu Gln Val Gly Lys Lys Leu Arg Gly Tyr 
465                 470                 475                 480 


Met Thr Asp Met Lys Arg Ile Ala Val Ala Gly 
                485                 490     


<210>  36
<211>  1851
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1851)

<400>  36
atg cct aag tac cgt tcc gcc acc acc act cat ggt cgt aat atg gcg         48
Met Pro Lys Tyr Arg Ser Ala Thr Thr Thr His Gly Arg Asn Met Ala           
1               5                   10                  15                

ggt gct cgt gcg ctg tgg cgc gcc acc gga atg acc gac gcc gat ttc         96
Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Met Thr Asp Ala Asp Phe           
            20                  25                  30                    

ggt aag ccg att atc gcg gtt gtg aac tcg ttc acc caa ttt gta ccg        144
Gly Lys Pro Ile Ile Ala Val Val Asn Ser Phe Thr Gln Phe Val Pro           
        35                  40                  45                        

ggt cac gtc cat ctg cgc gat ctc ggt aaa ctg gtc gcc gaa caa att        192
Gly His Val His Leu Arg Asp Leu Gly Lys Leu Val Ala Glu Gln Ile           
    50                  55                  60                            

gaa gcg gct ggc ggc gtt gcc aaa gag ttc aac acc att gcg gtg gat        240
Glu Ala Ala Gly Gly Val Ala Lys Glu Phe Asn Thr Ile Ala Val Asp           
65                  70                  75                  80            

gat ggg att gcc atg ggc cac ggg ggg atg ctt tat tca ctg cca tct        288
Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser           
                85                  90                  95                

cgc gaa ctg atc gct gat tcc gtt gag tat atg gtc aac gcc cac tgc        336
Arg Glu Leu Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Cys           
            100                 105                 110                   

gcc gac gcc atg gtc tgc atc tct aac tgc gac aaa atc acc ccg ggg        384
Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly           
        115                 120                 125                       

atg ctg atg gct tcc ctg cgc ctg aat att ccg gtg atc ttt gtt tcc        432
Met Leu Met Ala Ser Leu Arg Leu Asn Ile Pro Val Ile Phe Val Ser           
    130                 135                 140                           

ggc ggc ccg atg gag gcc ggg aaa acc aaa ctt tcc gat cag atc atc        480
Gly Gly Pro Met Glu Ala Gly Lys Thr Lys Leu Ser Asp Gln Ile Ile           
145                 150                 155                 160           

aag ctc gat ctg gtt gat gcg atg atc cag ggc gca gac ccg aaa gta        528
Lys Leu Asp Leu Val Asp Ala Met Ile Gln Gly Ala Asp Pro Lys Val           
                165                 170                 175               

tct gac tcc cag agc gat cag gtt gaa cgt tcc gcg tgt ccg acc tgc        576
Ser Asp Ser Gln Ser Asp Gln Val Glu Arg Ser Ala Cys Pro Thr Cys           
            180                 185                 190                   

ggt tcc tgc tcc ggg atg ttt acc gct aac tca atg aac tgc ctg acc        624
Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys Leu Thr           
        195                 200                 205                       

gaa gcg ctg ggc ctg tcg cag ccg ggc aac ggc tcg ctg ctg gca acc        672
Glu Ala Leu Gly Leu Ser Gln Pro Gly Asn Gly Ser Leu Leu Ala Thr           
    210                 215                 220                           

cac gcc gac cgt aag cag ctg ttc ctt aat gct ggt aaa cgc att gtt        720
His Ala Asp Arg Lys Gln Leu Phe Leu Asn Ala Gly Lys Arg Ile Val           
225                 230                 235                 240           

gaa ttg acc aaa cgt tat tac gag caa aac gac gaa agt gca ctg ccg        768
Glu Leu Thr Lys Arg Tyr Tyr Glu Gln Asn Asp Glu Ser Ala Leu Pro           
                245                 250                 255               

cgt aat atc gcc agt aag gcg gcg ttt gaa aac gcc atg acg ctg gat        816
Arg Asn Ile Ala Ser Lys Ala Ala Phe Glu Asn Ala Met Thr Leu Asp           
            260                 265                 270                   

atc gcg atg ggt gga tcg act aac acc gta ctt cac ctg ctg gcg gcg        864
Ile Ala Met Gly Gly Ser Thr Asn Thr Val Leu His Leu Leu Ala Ala           
        275                 280                 285                       

gcg cag gaa gcg gaa atc gac ttc acc atg agt gat atc gat aag ctt        912
Ala Gln Glu Ala Glu Ile Asp Phe Thr Met Ser Asp Ile Asp Lys Leu           
    290                 295                 300                           

tcc cgc aag gtt cca cag ctg tgt aaa gtt gcg ccg agc acc cag aaa        960
Ser Arg Lys Val Pro Gln Leu Cys Lys Val Ala Pro Ser Thr Gln Lys           
305                 310                 315                 320           

tac cat atg gaa gat gtt cac cgt gct ggt ggt gtt atc ggt att ctc       1008
Tyr His Met Glu Asp Val His Arg Ala Gly Gly Val Ile Gly Ile Leu           
                325                 330                 335               

ggc gaa ctg gat cgc gcg ggg tta ctg aac cgt gat gtg aaa aac gta       1056
Gly Glu Leu Asp Arg Ala Gly Leu Leu Asn Arg Asp Val Lys Asn Val           
            340                 345                 350                   

ctt ggc ctg acg ttg ccg caa acg ctg gaa caa tac gac gtt atg ctg       1104
Leu Gly Leu Thr Leu Pro Gln Thr Leu Glu Gln Tyr Asp Val Met Leu           
        355                 360                 365                       

acc cag gat gac gcg gta aaa aat atg ttc cgc gca ggt cct gca ggc       1152
Thr Gln Asp Asp Ala Val Lys Asn Met Phe Arg Ala Gly Pro Ala Gly           
    370                 375                 380                           

att cgt acc aca cag gca ttc tcg caa gat tgc cgt tgg gat acg ctg       1200
Ile Arg Thr Thr Gln Ala Phe Ser Gln Asp Cys Arg Trp Asp Thr Leu           
385                 390                 395                 400           

gac gac gat cgc gcc aat ggc tgt atc cgc tcg ctg gaa cac gcc tac       1248
Asp Asp Asp Arg Ala Asn Gly Cys Ile Arg Ser Leu Glu His Ala Tyr           
                405                 410                 415               

agc aaa gac ggc ggc ctg gcg gtg ctc tac ggt aac ttt gcg gaa aac       1296
Ser Lys Asp Gly Gly Leu Ala Val Leu Tyr Gly Asn Phe Ala Glu Asn           
            420                 425                 430                   

ggc tgc atc gtg aaa acg gca ggc gtc gat gac agc atc ctc aaa ttc       1344
Gly Cys Ile Val Lys Thr Ala Gly Val Asp Asp Ser Ile Leu Lys Phe           
        435                 440                 445                       

acc ggc ccg gcg aaa gtg tac gaa agc cag gac gat gcg gta gaa gcg       1392
Thr Gly Pro Ala Lys Val Tyr Glu Ser Gln Asp Asp Ala Val Glu Ala           
    450                 455                 460                           

att ctc ggc ggt aaa gtt gtc gcc gga gat gtg gta gta att cgc tat       1440
Ile Leu Gly Gly Lys Val Val Ala Gly Asp Val Val Val Ile Arg Tyr           
465                 470                 475                 480           

gaa ggc ccg aaa ggc ggt ccg ggg atg cag gaa atg ctc tac cca acc       1488
Glu Gly Pro Lys Gly Gly Pro Gly Met Gln Glu Met Leu Tyr Pro Thr           
                485                 490                 495               

agc ttc ctg aaa tca atg ggt ctc ggc aaa gcc tgt gcg ctg atc acc       1536
Ser Phe Leu Lys Ser Met Gly Leu Gly Lys Ala Cys Ala Leu Ile Thr           
            500                 505                 510                   

gac ggt cgt ttc tct ggt ggc acc tct ggt ctt tcc atc ggc cac gtc       1584
Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly His Val           
        515                 520                 525                       

tca ccg gaa gcg gca agc ggc ggc agc att ggc ctg att gaa gat ggt       1632
Ser Pro Glu Ala Ala Ser Gly Gly Ser Ile Gly Leu Ile Glu Asp Gly           
    530                 535                 540                           

gac ctg atc gct atc gac atc ccg aac cgt ggc att cag tta cag gta       1680
Asp Leu Ile Ala Ile Asp Ile Pro Asn Arg Gly Ile Gln Leu Gln Val           
545                 550                 555                 560           

agc gat gcc gaa ctg gcg gcg cgt cgt gaa gcg cag gac gct cga ggt       1728
Ser Asp Ala Glu Leu Ala Ala Arg Arg Glu Ala Gln Asp Ala Arg Gly           
                565                 570                 575               

gac aaa gcc tgg acg ccg aaa aat cgt gaa cgt cag gtc tcc ttt gcc       1776
Asp Lys Ala Trp Thr Pro Lys Asn Arg Glu Arg Gln Val Ser Phe Ala           
            580                 585                 590                   

ctg cgt gct tat gcc agc ctg gca acc agc gcc gac aaa ggc gcg gtg       1824
Leu Arg Ala Tyr Ala Ser Leu Ala Thr Ser Ala Asp Lys Gly Ala Val           
        595                 600                 605                       

cgc gat aaa tcg aaa ctg ggg ggt taa                                   1851
Arg Asp Lys Ser Lys Leu Gly Gly                                           
    610                 615                                               


<210>  37
<211>  616
<212>  PRT
<213>  Escherichia coli

<400>  37

Met Pro Lys Tyr Arg Ser Ala Thr Thr Thr His Gly Arg Asn Met Ala 
1               5                   10                  15      


Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Met Thr Asp Ala Asp Phe 
            20                  25                  30          


Gly Lys Pro Ile Ile Ala Val Val Asn Ser Phe Thr Gln Phe Val Pro 
        35                  40                  45              


Gly His Val His Leu Arg Asp Leu Gly Lys Leu Val Ala Glu Gln Ile 
    50                  55                  60                  


Glu Ala Ala Gly Gly Val Ala Lys Glu Phe Asn Thr Ile Ala Val Asp 
65                  70                  75                  80  


Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser 
                85                  90                  95      


Arg Glu Leu Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Cys 
            100                 105                 110         


Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly 
        115                 120                 125             


Met Leu Met Ala Ser Leu Arg Leu Asn Ile Pro Val Ile Phe Val Ser 
    130                 135                 140                 


Gly Gly Pro Met Glu Ala Gly Lys Thr Lys Leu Ser Asp Gln Ile Ile 
145                 150                 155                 160 


Lys Leu Asp Leu Val Asp Ala Met Ile Gln Gly Ala Asp Pro Lys Val 
                165                 170                 175     


Ser Asp Ser Gln Ser Asp Gln Val Glu Arg Ser Ala Cys Pro Thr Cys 
            180                 185                 190         


Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys Leu Thr 
        195                 200                 205             


Glu Ala Leu Gly Leu Ser Gln Pro Gly Asn Gly Ser Leu Leu Ala Thr 
    210                 215                 220                 


His Ala Asp Arg Lys Gln Leu Phe Leu Asn Ala Gly Lys Arg Ile Val 
225                 230                 235                 240 


Glu Leu Thr Lys Arg Tyr Tyr Glu Gln Asn Asp Glu Ser Ala Leu Pro 
                245                 250                 255     


Arg Asn Ile Ala Ser Lys Ala Ala Phe Glu Asn Ala Met Thr Leu Asp 
            260                 265                 270         


Ile Ala Met Gly Gly Ser Thr Asn Thr Val Leu His Leu Leu Ala Ala 
        275                 280                 285             


Ala Gln Glu Ala Glu Ile Asp Phe Thr Met Ser Asp Ile Asp Lys Leu 
    290                 295                 300                 


Ser Arg Lys Val Pro Gln Leu Cys Lys Val Ala Pro Ser Thr Gln Lys 
305                 310                 315                 320 


Tyr His Met Glu Asp Val His Arg Ala Gly Gly Val Ile Gly Ile Leu 
                325                 330                 335     


Gly Glu Leu Asp Arg Ala Gly Leu Leu Asn Arg Asp Val Lys Asn Val 
            340                 345                 350         


Leu Gly Leu Thr Leu Pro Gln Thr Leu Glu Gln Tyr Asp Val Met Leu 
        355                 360                 365             


Thr Gln Asp Asp Ala Val Lys Asn Met Phe Arg Ala Gly Pro Ala Gly 
    370                 375                 380                 


Ile Arg Thr Thr Gln Ala Phe Ser Gln Asp Cys Arg Trp Asp Thr Leu 
385                 390                 395                 400 


Asp Asp Asp Arg Ala Asn Gly Cys Ile Arg Ser Leu Glu His Ala Tyr 
                405                 410                 415     


Ser Lys Asp Gly Gly Leu Ala Val Leu Tyr Gly Asn Phe Ala Glu Asn 
            420                 425                 430         


Gly Cys Ile Val Lys Thr Ala Gly Val Asp Asp Ser Ile Leu Lys Phe 
        435                 440                 445             


Thr Gly Pro Ala Lys Val Tyr Glu Ser Gln Asp Asp Ala Val Glu Ala 
    450                 455                 460                 


Ile Leu Gly Gly Lys Val Val Ala Gly Asp Val Val Val Ile Arg Tyr 
465                 470                 475                 480 


Glu Gly Pro Lys Gly Gly Pro Gly Met Gln Glu Met Leu Tyr Pro Thr 
                485                 490                 495     


Ser Phe Leu Lys Ser Met Gly Leu Gly Lys Ala Cys Ala Leu Ile Thr 
            500                 505                 510         


Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly His Val 
        515                 520                 525             


Ser Pro Glu Ala Ala Ser Gly Gly Ser Ile Gly Leu Ile Glu Asp Gly 
    530                 535                 540                 


Asp Leu Ile Ala Ile Asp Ile Pro Asn Arg Gly Ile Gln Leu Gln Val 
545                 550                 555                 560 


Ser Asp Ala Glu Leu Ala Ala Arg Arg Glu Ala Gln Asp Ala Arg Gly 
                565                 570                 575     


Asp Lys Ala Trp Thr Pro Lys Asn Arg Glu Arg Gln Val Ser Phe Ala 
            580                 585                 590         


Leu Arg Ala Tyr Ala Ser Leu Ala Thr Ser Ala Asp Lys Gly Ala Val 
        595                 600                 605             


Arg Asp Lys Ser Lys Leu Gly Gly 
    610                 615     


<210>  38
<211>  1545
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1545)

<400>  38
atg gct gac tcg caa ccc ctg tcc ggt gct ccg gaa ggt gcc gaa tat         48
Met Ala Asp Ser Gln Pro Leu Ser Gly Ala Pro Glu Gly Ala Glu Tyr           
1               5                   10                  15                

tta aga gca gtg ctg cgc gcg ccg gtt tac gag gcg gcg cag gtt acg         96
Leu Arg Ala Val Leu Arg Ala Pro Val Tyr Glu Ala Ala Gln Val Thr           
            20                  25                  30                    

ccg cta caa aaa atg gaa aaa ctg tcg tcg cgt ctt gat aac gtc att        144
Pro Leu Gln Lys Met Glu Lys Leu Ser Ser Arg Leu Asp Asn Val Ile           
        35                  40                  45                        

ctg gtg aag cgc gaa gat cgc cag cca gtg cac agc ttt aag ctg cgc        192
Leu Val Lys Arg Glu Asp Arg Gln Pro Val His Ser Phe Lys Leu Arg           
    50                  55                  60                            

ggc gca tac gcc atg atg gcg ggc ctg acg gaa gaa cag aaa gcg cac        240
Gly Ala Tyr Ala Met Met Ala Gly Leu Thr Glu Glu Gln Lys Ala His           
65                  70                  75                  80            

ggc gtg atc act gct tct gcg ggt aac cac gcg cag ggc gtc gcg ttt        288
Gly Val Ile Thr Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala Phe           
                85                  90                  95                

tct tct gcg cgg tta ggc gtg aag gcc ctg atc gtt atg cca acc gcc        336
Ser Ser Ala Arg Leu Gly Val Lys Ala Leu Ile Val Met Pro Thr Ala           
            100                 105                 110                   

acc gcc gac atc aaa gtc gac gcg gtg cgc ggc ttc ggc ggc gaa gtg        384
Thr Ala Asp Ile Lys Val Asp Ala Val Arg Gly Phe Gly Gly Glu Val           
        115                 120                 125                       

ctg ctc cac ggc gcg aac ttt gat gaa gcg aaa gcc aaa gcg atc gaa        432
Leu Leu His Gly Ala Asn Phe Asp Glu Ala Lys Ala Lys Ala Ile Glu           
    130                 135                 140                           

ctg tca cag cag cag ggg ttc acc tgg gtg ccg ccg ttc gac cat ccg        480
Leu Ser Gln Gln Gln Gly Phe Thr Trp Val Pro Pro Phe Asp His Pro           
145                 150                 155                 160           

atg gtg att gcc ggg caa ggc acg ctg gcg ctg gaa ctg ctc cag cag        528
Met Val Ile Ala Gly Gln Gly Thr Leu Ala Leu Glu Leu Leu Gln Gln           
                165                 170                 175               

gac gcc cat ctc gac cgc gta ttt gtg cca gtc ggc ggc ggc ggt ctg        576
Asp Ala His Leu Asp Arg Val Phe Val Pro Val Gly Gly Gly Gly Leu           
            180                 185                 190                   

gct gct ggc gtg gcg gtg ctg atc aaa caa ctg atg ccg caa atc aaa        624
Ala Ala Gly Val Ala Val Leu Ile Lys Gln Leu Met Pro Gln Ile Lys           
        195                 200                 205                       

gtg atc gcc gta gaa gcg gaa gac tcc gcc tgc ctg aaa gca gcg ctg        672
Val Ile Ala Val Glu Ala Glu Asp Ser Ala Cys Leu Lys Ala Ala Leu           
    210                 215                 220                           

gat gcg ggt cat ccg gtt gat ctg ccg cgc gta ggg cta ttt gct gaa        720
Asp Ala Gly His Pro Val Asp Leu Pro Arg Val Gly Leu Phe Ala Glu           
225                 230                 235                 240           

ggc gta gcg gta aaa cgc atc ggt gac gaa acc ttc cgt tta tgc cag        768
Gly Val Ala Val Lys Arg Ile Gly Asp Glu Thr Phe Arg Leu Cys Gln           
                245                 250                 255               

gag tat ctc gac gac atc atc acc gtc gat agc gat gcg atc tgt gcg        816
Glu Tyr Leu Asp Asp Ile Ile Thr Val Asp Ser Asp Ala Ile Cys Ala           
            260                 265                 270                   

gcg atg aag gat tta ttc gaa gat gtg cgc gcg gtg gcg gaa ccc tct        864
Ala Met Lys Asp Leu Phe Glu Asp Val Arg Ala Val Ala Glu Pro Ser           
        275                 280                 285                       

ggc gcg ctg gcg ctg gcg gga atg aaa aaa tat atc gcc ctg cac aac        912
Gly Ala Leu Ala Leu Ala Gly Met Lys Lys Tyr Ile Ala Leu His Asn           
    290                 295                 300                           

att cgc ggc gaa cgg ctg gcg cat att ctt tcc ggt gcc aac gtg aac        960
Ile Arg Gly Glu Arg Leu Ala His Ile Leu Ser Gly Ala Asn Val Asn           
305                 310                 315                 320           

ttc cac ggc ctg cgc tac gtc tca gaa cgc tgc gaa ctg ggc gaa cag       1008
Phe His Gly Leu Arg Tyr Val Ser Glu Arg Cys Glu Leu Gly Glu Gln           
                325                 330                 335               

cgt gaa gcg ttg ttg gcg gtg acc att ccg gaa gaa aaa ggc agc ttc       1056
Arg Glu Ala Leu Leu Ala Val Thr Ile Pro Glu Glu Lys Gly Ser Phe           
            340                 345                 350                   

ctc aaa ttc tgc caa ctg ctt ggc ggg cgt tcg gtc acc gag ttc aac       1104
Leu Lys Phe Cys Gln Leu Leu Gly Gly Arg Ser Val Thr Glu Phe Asn           
        355                 360                 365                       

tac cgt ttt gcc gat gcc aaa aac gcc tgc atc ttt gtc ggt gtg cgc       1152
Tyr Arg Phe Ala Asp Ala Lys Asn Ala Cys Ile Phe Val Gly Val Arg           
    370                 375                 380                           

ctg agc cgc ggc ctc gaa gag cgc aaa gaa att ttg cag atg ctc aac       1200
Leu Ser Arg Gly Leu Glu Glu Arg Lys Glu Ile Leu Gln Met Leu Asn           
385                 390                 395                 400           

gac ggc ggc tac agc gtg gtt gat ctc tcc gac gac gaa atg gcg aag       1248
Asp Gly Gly Tyr Ser Val Val Asp Leu Ser Asp Asp Glu Met Ala Lys           
                405                 410                 415               

cta cac gtg cgc tat atg gtc ggc gga cgt cca tcg cat ccg ttg cag       1296
Leu His Val Arg Tyr Met Val Gly Gly Arg Pro Ser His Pro Leu Gln           
            420                 425                 430                   

gaa cgc ctc tac agc ttc gaa ttc ccg gaa tca ccg ggc gcg ctg ctg       1344
Glu Arg Leu Tyr Ser Phe Glu Phe Pro Glu Ser Pro Gly Ala Leu Leu           
        435                 440                 445                       

cgc ttc ctc aac acg ctg ggt acg tac tgg aac att tct ttg ttc cac       1392
Arg Phe Leu Asn Thr Leu Gly Thr Tyr Trp Asn Ile Ser Leu Phe His           
    450                 455                 460                           

tat cgc agc cat ggc acc gac tac ggg cgc gta ctg gcg gcg ttc gaa       1440
Tyr Arg Ser His Gly Thr Asp Tyr Gly Arg Val Leu Ala Ala Phe Glu           
465                 470                 475                 480           

ctt ggc gac cat gaa ccg gat ttc gaa acc cgg ctg aat gag ctg ggc       1488
Leu Gly Asp His Glu Pro Asp Phe Glu Thr Arg Leu Asn Glu Leu Gly           
                485                 490                 495               

tac gat tgc cac gac gaa acc aat aac ccg gcg ttc agg ttc ttt ttg       1536
Tyr Asp Cys His Asp Glu Thr Asn Asn Pro Ala Phe Arg Phe Phe Leu           
            500                 505                 510                   

gcg ggt tag                                                           1545
Ala Gly                                                                   
                                                                          


<210>  39
<211>  514
<212>  PRT
<213>  Escherichia coli

<400>  39

Met Ala Asp Ser Gln Pro Leu Ser Gly Ala Pro Glu Gly Ala Glu Tyr 
1               5                   10                  15      


Leu Arg Ala Val Leu Arg Ala Pro Val Tyr Glu Ala Ala Gln Val Thr 
            20                  25                  30          


Pro Leu Gln Lys Met Glu Lys Leu Ser Ser Arg Leu Asp Asn Val Ile 
        35                  40                  45              


Leu Val Lys Arg Glu Asp Arg Gln Pro Val His Ser Phe Lys Leu Arg 
    50                  55                  60                  


Gly Ala Tyr Ala Met Met Ala Gly Leu Thr Glu Glu Gln Lys Ala His 
65                  70                  75                  80  


Gly Val Ile Thr Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala Phe 
                85                  90                  95      


Ser Ser Ala Arg Leu Gly Val Lys Ala Leu Ile Val Met Pro Thr Ala 
            100                 105                 110         


Thr Ala Asp Ile Lys Val Asp Ala Val Arg Gly Phe Gly Gly Glu Val 
        115                 120                 125             


Leu Leu His Gly Ala Asn Phe Asp Glu Ala Lys Ala Lys Ala Ile Glu 
    130                 135                 140                 


Leu Ser Gln Gln Gln Gly Phe Thr Trp Val Pro Pro Phe Asp His Pro 
145                 150                 155                 160 


Met Val Ile Ala Gly Gln Gly Thr Leu Ala Leu Glu Leu Leu Gln Gln 
                165                 170                 175     


Asp Ala His Leu Asp Arg Val Phe Val Pro Val Gly Gly Gly Gly Leu 
            180                 185                 190         


Ala Ala Gly Val Ala Val Leu Ile Lys Gln Leu Met Pro Gln Ile Lys 
        195                 200                 205             


Val Ile Ala Val Glu Ala Glu Asp Ser Ala Cys Leu Lys Ala Ala Leu 
    210                 215                 220                 


Asp Ala Gly His Pro Val Asp Leu Pro Arg Val Gly Leu Phe Ala Glu 
225                 230                 235                 240 


Gly Val Ala Val Lys Arg Ile Gly Asp Glu Thr Phe Arg Leu Cys Gln 
                245                 250                 255     


Glu Tyr Leu Asp Asp Ile Ile Thr Val Asp Ser Asp Ala Ile Cys Ala 
            260                 265                 270         


Ala Met Lys Asp Leu Phe Glu Asp Val Arg Ala Val Ala Glu Pro Ser 
        275                 280                 285             


Gly Ala Leu Ala Leu Ala Gly Met Lys Lys Tyr Ile Ala Leu His Asn 
    290                 295                 300                 


Ile Arg Gly Glu Arg Leu Ala His Ile Leu Ser Gly Ala Asn Val Asn 
305                 310                 315                 320 


Phe His Gly Leu Arg Tyr Val Ser Glu Arg Cys Glu Leu Gly Glu Gln 
                325                 330                 335     


Arg Glu Ala Leu Leu Ala Val Thr Ile Pro Glu Glu Lys Gly Ser Phe 
            340                 345                 350         


Leu Lys Phe Cys Gln Leu Leu Gly Gly Arg Ser Val Thr Glu Phe Asn 
        355                 360                 365             


Tyr Arg Phe Ala Asp Ala Lys Asn Ala Cys Ile Phe Val Gly Val Arg 
    370                 375                 380                 


Leu Ser Arg Gly Leu Glu Glu Arg Lys Glu Ile Leu Gln Met Leu Asn 
385                 390                 395                 400 


Asp Gly Gly Tyr Ser Val Val Asp Leu Ser Asp Asp Glu Met Ala Lys 
                405                 410                 415     


Leu His Val Arg Tyr Met Val Gly Gly Arg Pro Ser His Pro Leu Gln 
            420                 425                 430         


Glu Arg Leu Tyr Ser Phe Glu Phe Pro Glu Ser Pro Gly Ala Leu Leu 
        435                 440                 445             


Arg Phe Leu Asn Thr Leu Gly Thr Tyr Trp Asn Ile Ser Leu Phe His 
    450                 455                 460                 


Tyr Arg Ser His Gly Thr Asp Tyr Gly Arg Val Leu Ala Ala Phe Glu 
465                 470                 475                 480 


Leu Gly Asp His Glu Pro Asp Phe Glu Thr Arg Leu Asn Glu Leu Gly 
                485                 490                 495     


Tyr Asp Cys His Asp Glu Thr Asn Asn Pro Ala Phe Arg Phe Phe Leu 
            500                 505                 510         


Ala Gly 
        


<210>  40
<211>  1572
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1572)

<400>  40
atg agc cag caa gtc att att ttc gat acc aca ttg cgc gac ggt gaa         48
Met Ser Gln Gln Val Ile Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu           
1               5                   10                  15                

cag gcg tta cag gca agc ttg agt gtg aaa gaa aaa ctg caa att gcg         96
Gln Ala Leu Gln Ala Ser Leu Ser Val Lys Glu Lys Leu Gln Ile Ala           
            20                  25                  30                    

ctg gcc ctt gag cgt atg ggt gtt gac gtg atg gaa gtc ggt ttc ccc        144
Leu Ala Leu Glu Arg Met Gly Val Asp Val Met Glu Val Gly Phe Pro           
        35                  40                  45                        

gtc tct tcg ccg ggc gat ttt gaa tcg gtg caa acc atc gcc cgc cag        192
Val Ser Ser Pro Gly Asp Phe Glu Ser Val Gln Thr Ile Ala Arg Gln           
    50                  55                  60                            

gtt aaa aac agc cgc gta tgt gcg tta gct cgc tgc gtg gaa aaa gat        240
Val Lys Asn Ser Arg Val Cys Ala Leu Ala Arg Cys Val Glu Lys Asp           
65                  70                  75                  80            

atc gac gtg gcg gcc gaa tcc ctg aaa gtc gcc gaa gcc ttc cgt att        288
Ile Asp Val Ala Ala Glu Ser Leu Lys Val Ala Glu Ala Phe Arg Ile           
                85                  90                  95                

cat acc ttt att gcc act tcg cca atg cac atc gcc acc aag ctg cgc        336
His Thr Phe Ile Ala Thr Ser Pro Met His Ile Ala Thr Lys Leu Arg           
            100                 105                 110                   

agc acg ctg gac gag gtg atc gaa cgc gct atc tat atg gtg aaa cgc        384
Ser Thr Leu Asp Glu Val Ile Glu Arg Ala Ile Tyr Met Val Lys Arg           
        115                 120                 125                       

gcc cgt aat tac acc gat gat gtt gaa ttt tct tgc gaa gat gcc ggg        432
Ala Arg Asn Tyr Thr Asp Asp Val Glu Phe Ser Cys Glu Asp Ala Gly           
    130                 135                 140                           

cgt aca ccc att gcc gat ctg gcg cga gtg gtc gaa gcg gcg att aat        480
Arg Thr Pro Ile Ala Asp Leu Ala Arg Val Val Glu Ala Ala Ile Asn           
145                 150                 155                 160           

gcc ggt gcc acc acc atc aac att ccg gac acc gtg ggc tac acc atg        528
Ala Gly Ala Thr Thr Ile Asn Ile Pro Asp Thr Val Gly Tyr Thr Met           
                165                 170                 175               

ccg ttt gag ttc gcc gga atc atc agc ggc ctg tat gaa cgc gtg cct        576
Pro Phe Glu Phe Ala Gly Ile Ile Ser Gly Leu Tyr Glu Arg Val Pro           
            180                 185                 190                   

aac atc gac aaa gcc att atc tcc gta cat acc cac gac gat ttg ggc        624
Asn Ile Asp Lys Ala Ile Ile Ser Val His Thr His Asp Asp Leu Gly           
        195                 200                 205                       

ctg gcg gtc gga aac tca ctg gcg gcg gta cat gcc ggt gca cgc cag        672
Leu Ala Val Gly Asn Ser Leu Ala Ala Val His Ala Gly Ala Arg Gln           
    210                 215                 220                           

gtg gaa ggc gca atg aac ggg atc ggc gag cgt gcc gga aac tgt tcc        720
Val Glu Gly Ala Met Asn Gly Ile Gly Glu Arg Ala Gly Asn Cys Ser           
225                 230                 235                 240           

ctg gaa gaa gtc atc atg gcg atc aaa gtt cgt aag gat att ctc aac        768
Leu Glu Glu Val Ile Met Ala Ile Lys Val Arg Lys Asp Ile Leu Asn           
                245                 250                 255               

gtc cac acc gcc att aat cac cag gag ata tgg cgc acc agc cag tta        816
Val His Thr Ala Ile Asn His Gln Glu Ile Trp Arg Thr Ser Gln Leu           
            260                 265                 270                   

gtt agc cag att tgt aat atg ccg atc ccg gca aac aaa gcc att gtt        864
Val Ser Gln Ile Cys Asn Met Pro Ile Pro Ala Asn Lys Ala Ile Val           
        275                 280                 285                       

ggc agc ggc gca ttc gca cac tcc tcc ggt ata cac cag gat ggc gtg        912
Gly Ser Gly Ala Phe Ala His Ser Ser Gly Ile His Gln Asp Gly Val           
    290                 295                 300                           

ctg aaa aac cgc gaa aac tac gaa atc atg aca cca gaa tct att ggt        960
Leu Lys Asn Arg Glu Asn Tyr Glu Ile Met Thr Pro Glu Ser Ile Gly           
305                 310                 315                 320           

ctg aac caa atc cag ctg aat ctg acc tct cgt tcg ggg cgt gcg gcg       1008
Leu Asn Gln Ile Gln Leu Asn Leu Thr Ser Arg Ser Gly Arg Ala Ala           
                325                 330                 335               

gtg aaa cat cgc atg gat gag atg ggg tat aaa gaa agt gaa tat aat       1056
Val Lys His Arg Met Asp Glu Met Gly Tyr Lys Glu Ser Glu Tyr Asn           
            340                 345                 350                   

tta gac aat ttg tac gat gct ttc ctg aag ctg gcg gac aaa aaa ggt       1104
Leu Asp Asn Leu Tyr Asp Ala Phe Leu Lys Leu Ala Asp Lys Lys Gly           
        355                 360                 365                       

cag gtg ttt gat tac gat ctg gag gcg ctg gcc ttc atc ggt aag cag       1152
Gln Val Phe Asp Tyr Asp Leu Glu Ala Leu Ala Phe Ile Gly Lys Gln           
    370                 375                 380                           

caa gaa gag ccg gag cat ttc cgt ctg gat tac ttc agc gtg cag tct       1200
Gln Glu Glu Pro Glu His Phe Arg Leu Asp Tyr Phe Ser Val Gln Ser           
385                 390                 395                 400           

ggc tct aac gat atc gcc acc gcc gcc gtc aaa ctg gcc tgt ggc gaa       1248
Gly Ser Asn Asp Ile Ala Thr Ala Ala Val Lys Leu Ala Cys Gly Glu           
                405                 410                 415               

gaa gtc aaa gca gaa gcc gcc aac ggt aac ggt ccg gtc gat gcc gtc       1296
Glu Val Lys Ala Glu Ala Ala Asn Gly Asn Gly Pro Val Asp Ala Val           
            420                 425                 430                   

tat cag gca att aac cgc atc act gaa tat aac gtc gaa ctg gtg aaa       1344
Tyr Gln Ala Ile Asn Arg Ile Thr Glu Tyr Asn Val Glu Leu Val Lys           
        435                 440                 445                       

tac agc ctg acc gcc aaa ggc cac ggt aaa gat gcg ctg ggt cag gtg       1392
Tyr Ser Leu Thr Ala Lys Gly His Gly Lys Asp Ala Leu Gly Gln Val           
    450                 455                 460                           

gat atc gtc gct aac tac aac ggt cgc cgc ttc cac ggc gtc ggc ctg       1440
Asp Ile Val Ala Asn Tyr Asn Gly Arg Arg Phe His Gly Val Gly Leu           
465                 470                 475                 480           

gct acc gat att gtc gag tca tct gcc aaa gcc atg gtg cac gtt ctg       1488
Ala Thr Asp Ile Val Glu Ser Ser Ala Lys Ala Met Val His Val Leu           
                485                 490                 495               

aac aat atc tgg cgt gcc gca gaa gtc gaa aaa gag ttg caa cgc aaa       1536
Asn Asn Ile Trp Arg Ala Ala Glu Val Glu Lys Glu Leu Gln Arg Lys           
            500                 505                 510                   

gct caa cac aac gaa aac aac aag gaa acc gtg tga                       1572
Ala Gln His Asn Glu Asn Asn Lys Glu Thr Val                               
        515                 520                                           


<210>  41
<211>  523
<212>  PRT
<213>  Escherichia coli

<400>  41

Met Ser Gln Gln Val Ile Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu 
1               5                   10                  15      


Gln Ala Leu Gln Ala Ser Leu Ser Val Lys Glu Lys Leu Gln Ile Ala 
            20                  25                  30          


Leu Ala Leu Glu Arg Met Gly Val Asp Val Met Glu Val Gly Phe Pro 
        35                  40                  45              


Val Ser Ser Pro Gly Asp Phe Glu Ser Val Gln Thr Ile Ala Arg Gln 
    50                  55                  60                  


Val Lys Asn Ser Arg Val Cys Ala Leu Ala Arg Cys Val Glu Lys Asp 
65                  70                  75                  80  


Ile Asp Val Ala Ala Glu Ser Leu Lys Val Ala Glu Ala Phe Arg Ile 
                85                  90                  95      


His Thr Phe Ile Ala Thr Ser Pro Met His Ile Ala Thr Lys Leu Arg 
            100                 105                 110         


Ser Thr Leu Asp Glu Val Ile Glu Arg Ala Ile Tyr Met Val Lys Arg 
        115                 120                 125             


Ala Arg Asn Tyr Thr Asp Asp Val Glu Phe Ser Cys Glu Asp Ala Gly 
    130                 135                 140                 


Arg Thr Pro Ile Ala Asp Leu Ala Arg Val Val Glu Ala Ala Ile Asn 
145                 150                 155                 160 


Ala Gly Ala Thr Thr Ile Asn Ile Pro Asp Thr Val Gly Tyr Thr Met 
                165                 170                 175     


Pro Phe Glu Phe Ala Gly Ile Ile Ser Gly Leu Tyr Glu Arg Val Pro 
            180                 185                 190         


Asn Ile Asp Lys Ala Ile Ile Ser Val His Thr His Asp Asp Leu Gly 
        195                 200                 205             


Leu Ala Val Gly Asn Ser Leu Ala Ala Val His Ala Gly Ala Arg Gln 
    210                 215                 220                 


Val Glu Gly Ala Met Asn Gly Ile Gly Glu Arg Ala Gly Asn Cys Ser 
225                 230                 235                 240 


Leu Glu Glu Val Ile Met Ala Ile Lys Val Arg Lys Asp Ile Leu Asn 
                245                 250                 255     


Val His Thr Ala Ile Asn His Gln Glu Ile Trp Arg Thr Ser Gln Leu 
            260                 265                 270         


Val Ser Gln Ile Cys Asn Met Pro Ile Pro Ala Asn Lys Ala Ile Val 
        275                 280                 285             


Gly Ser Gly Ala Phe Ala His Ser Ser Gly Ile His Gln Asp Gly Val 
    290                 295                 300                 


Leu Lys Asn Arg Glu Asn Tyr Glu Ile Met Thr Pro Glu Ser Ile Gly 
305                 310                 315                 320 


Leu Asn Gln Ile Gln Leu Asn Leu Thr Ser Arg Ser Gly Arg Ala Ala 
                325                 330                 335     


Val Lys His Arg Met Asp Glu Met Gly Tyr Lys Glu Ser Glu Tyr Asn 
            340                 345                 350         


Leu Asp Asn Leu Tyr Asp Ala Phe Leu Lys Leu Ala Asp Lys Lys Gly 
        355                 360                 365             


Gln Val Phe Asp Tyr Asp Leu Glu Ala Leu Ala Phe Ile Gly Lys Gln 
    370                 375                 380                 


Gln Glu Glu Pro Glu His Phe Arg Leu Asp Tyr Phe Ser Val Gln Ser 
385                 390                 395                 400 


Gly Ser Asn Asp Ile Ala Thr Ala Ala Val Lys Leu Ala Cys Gly Glu 
                405                 410                 415     


Glu Val Lys Ala Glu Ala Ala Asn Gly Asn Gly Pro Val Asp Ala Val 
            420                 425                 430         


Tyr Gln Ala Ile Asn Arg Ile Thr Glu Tyr Asn Val Glu Leu Val Lys 
        435                 440                 445             


Tyr Ser Leu Thr Ala Lys Gly His Gly Lys Asp Ala Leu Gly Gln Val 
    450                 455                 460                 


Asp Ile Val Ala Asn Tyr Asn Gly Arg Arg Phe His Gly Val Gly Leu 
465                 470                 475                 480 


Ala Thr Asp Ile Val Glu Ser Ser Ala Lys Ala Met Val His Val Leu 
                485                 490                 495     


Asn Asn Ile Trp Arg Ala Ala Glu Val Glu Lys Glu Leu Gln Arg Lys 
            500                 505                 510         


Ala Gln His Asn Glu Asn Asn Lys Glu Thr Val 
        515                 520             


<210>  42
<211>  1095
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1095)

<400>  42
gtg atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att         48
Val Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile           
1               5                   10                  15                

ggt ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc         96
Gly Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg           
            20                  25                  30                    

aac cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc        144
Asn Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly           
        35                  40                  45                        

gca gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa        192
Ala Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu           
    50                  55                  60                            

ggt tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg        240
Gly Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro           
65                  70                  75                  80            

aag tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg        288
Lys Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu           
                85                  90                  95                

ctg cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca        336
Leu Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala           
            100                 105                 110                   

aaa ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att        384
Lys Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile           
        115                 120                 125                       

gcc gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc        432
Ala Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly           
    130                 135                 140                           

atc tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa        480
Ile Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu           
145                 150                 155                 160           

aaa gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc        528
Lys Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile           
                165                 170                 175               

gcc cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg        576
Ala Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr           
            180                 185                 190                   

tcg atc gat aaa gcc aac gtg ctg caa tcc tct att tta tgg cgg gag        624
Ser Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu           
        195                 200                 205                       

atc gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cat        672
Ile Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His           
    210                 215                 220                           

atg tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag        720
Met Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln           
225                 230                 235                 240           

ttt gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac        768
Phe Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp           
                245                 250                 255               

gag tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc        816
Glu Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser           
            260                 265                 270                   

ctg aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca        864
Leu Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala           
        275                 280                 285                       

cca gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt        912
Pro Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu           
    290                 295                 300                           

tcg ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct        960
Ser Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala           
305                 310                 315                 320           

tgc gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc       1008
Cys Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg           
                325                 330                 335               

acc ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg       1056
Thr Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met           
            340                 345                 350                   

ggc gat atc att gcc cgc tat gta gca gaa ggg gtg taa                   1095
Gly Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                           
        355                 360                                           


<210>  43
<211>  364
<212>  PRT
<213>  Escherichia coli

<400>  43

Val Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile 
1               5                   10                  15      


Gly Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg 
            20                  25                  30          


Asn Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly 
        35                  40                  45              


Ala Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu 
    50                  55                  60                  


Gly Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro 
65                  70                  75                  80  


Lys Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu 
                85                  90                  95      


Leu Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala 
            100                 105                 110         


Lys Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile 
        115                 120                 125             


Ala Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly 
    130                 135                 140                 


Ile Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu 
145                 150                 155                 160 


Lys Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile 
                165                 170                 175     


Ala Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr 
            180                 185                 190         


Ser Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu 
        195                 200                 205             


Ile Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His 
    210                 215                 220                 


Met Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln 
225                 230                 235                 240 


Phe Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp 
                245                 250                 255     


Glu Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser 
            260                 265                 270         


Leu Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala 
        275                 280                 285             


Pro Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu 
    290                 295                 300                 


Ser Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala 
305                 310                 315                 320 


Cys Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg 
                325                 330                 335     


Thr Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met 
            340                 345                 350         


Gly Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360                 


<210>  44
<211>  1401
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1401)

<400>  44
atg gct aag acg tta tac gaa aaa ttg ttc gac gct cac gtt gtg tac         48
Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr           
1               5                   10                  15                

gaa gcc gaa aac gaa acc cca ctg tta tat atc gac cgc cac ctg gtg         96
Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val           
            20                  25                  30                    

cat gaa gtg acc tca ccg cag gcg ttc gat ggt ctg cgc gcc cac ggt        144
His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly           
        35                  40                  45                        

cgc ccg gta cgt cag ccg ggc aaa acc ttc gct acc atg gat cac aac        192
Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn           
    50                  55                  60                            

gtc tct acc cag acc aaa gac att aat gcc tgc ggt gaa atg gcg cgt        240
Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg           
65                  70                  75                  80            

atc cag atg cag gaa ctg atc aaa aac tgc aaa gaa ttt ggc gtc gaa        288
Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu           
                85                  90                  95                

ctg tat gac ctg aat cac ccg tat cag ggg atc gtc cac gta atg ggg        336
Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly           
            100                 105                 110                   

ccg gaa cag ggc gtc acc ttg ccg ggg atg acc att gtc tgc ggc gac        384
Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp           
        115                 120                 125                       

tcg cat acc gcc acc cac ggc gcg ttt ggc gca ctg gcc ttt ggt atc        432
Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile           
    130                 135                 140                           

ggc act tcc gaa gtt gaa cac gta ctg gca acg caa acc ctg aaa cag        480
Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln           
145                 150                 155                 160           

ggc cgc gca aaa acc atg aaa att gaa gtc cag ggc aaa gcc gcg ccg        528
Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro           
                165                 170                 175               

ggc att acc gca aaa gat atc gtg ctg gca att atc ggt aaa acc ggt        576
Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly           
            180                 185                 190                   

agc gca ggc ggc acc ggg cat gtg gtg gag ttt tgc ggc gaa gca atc        624
Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile           
        195                 200                 205                       

cgt gat tta agc atg gaa ggt cgt atg acc ctg tgc aat atg gca atc        672
Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile           
    210                 215                 220                           

gaa atg ggc gca aaa gcc ggt ctg gtt gca ccg gac gaa acc acc ttt        720
Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe           
225                 230                 235                 240           

aac tat gtc aaa ggc cgt ctg cat gcg ccg aaa ggc aaa gat ttc gac        768
Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp           
                245                 250                 255               

gac gcc gtt gcc tac tgg aaa acc ctg caa acc gac gaa ggc gca act        816
Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr           
            260                 265                 270                   

ttc gat acc gtt gtc act ctg caa gca gaa gaa att tca ccg cag gtc        864
Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val           
        275                 280                 285                       

acc tgg ggc acc aat ccc ggc cag gtg att tcc gtg aac gac aat att        912
Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile           
    290                 295                 300                           

ccc gat ccg gct tcg ttt gcc gat ccg gtt gaa cgc gcg tcg gca gaa        960
Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu           
305                 310                 315                 320           

aaa gcg ctg gcc tat atg ggg ctg aaa ccg ggt att ccg ctg acc gaa       1008
Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu           
                325                 330                 335               

gtg gct atc gac aaa gtg ttt atc ggt tcc tgt acc aac tcg cgc att       1056
Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile           
            340                 345                 350                   

gaa gat tta cgc gcg gca gcg gag atc gcc aaa ggg cga aaa gtc gcg       1104
Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala           
        355                 360                 365                       

cca ggc gtg cag gca ctg gtg gtt ccc ggc tct ggc ccg gta aaa gcc       1152
Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala           
    370                 375                 380                           

cag gcg gaa gcg gaa ggt ctg gat aaa atc ttt att gaa gcc ggt ttt       1200
Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe           
385                 390                 395                 400           

gaa tgg cgc ttg cct ggc tgc tca atg tgt ctg gcg atg aac aac gac       1248
Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asn Asp           
                405                 410                 415               

cgt ctg aat ccg ggc gaa cgt tgt gcc tcc acc agc aac cgt aac ttt       1296
Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe           
            420                 425                 430                   

gaa ggc cgc cag ggg cgc ggc ggg cgc acg cat ctg gtc agc ccg gca       1344
Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala           
        435                 440                 445                       

atg gct gcc gct gct gct gtg acc gga cat ttc gcc gac att cgc aac       1392
Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn           
    450                 455                 460                           

att aaa taa                                                           1401
Ile Lys                                                                   
465                                                                       


<210>  45
<211>  466
<212>  PRT
<213>  Escherichia coli

<400>  45

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  46
<211>  606
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(606)

<400>  46
atg gca gag aaa ttt atc aaa cac aca ggc ctg gtg gtt ccg ctg gat         48
Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp           
1               5                   10                  15                

gcc gcc aat gtc gat acc gat gca atc atc ccg aaa cag ttt ttg cag         96
Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln           
            20                  25                  30                    

aaa gtg acc cgt acg ggt ttt ggc gcg cat ctg ttt aac gac tgg cgt        144
Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg           
        35                  40                  45                        

ttt ctg gat gaa aaa ggc caa cag cca aac ccg gac ttc gtg ctg aac        192
Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn           
    50                  55                  60                            

ttc ccg cag tat cag ggc gct tcc att ttg ctg gca cga gaa aac ttc        240
Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe           
65                  70                  75                  80            

ggc tgt ggc tct tcg cgt gag cac gcg ccc tgg gca ttg acc gac tac        288
Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr           
                85                  90                  95                

ggt ttt aaa gtg gtg att gcg ccg agt ttt gct gac atc ttc tac ggc        336
Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly           
            100                 105                 110                   

aat agc ttt aac aac cag ctg ctg ccg gtg aaa tta agc gat gca gaa        384
Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu           
        115                 120                 125                       

gtg gac gaa ctg ttt gcg ctg gtg aaa gct aat ccg ggg atc cat ttc        432
Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe           
    130                 135                 140                           

gac gtg gat ctg gaa gcg caa gag gtg aaa gcg gga gag aaa acc tat        480
Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr           
145                 150                 155                 160           

cgc ttt acc atc gat gcc ttc cgc cgc cac tgc atg atg aac ggt ctg        528
Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu           
                165                 170                 175               

gac agt att ggg ctt acc ttg cag cac gac gac gcc att gcc gct tat        576
Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr           
            180                 185                 190                   

gaa gca aaa caa cct gcg ttt atg aat taa                                606
Glu Ala Lys Gln Pro Ala Phe Met Asn                                       
        195                 200                                           


<210>  47
<211>  201
<212>  PRT
<213>  Escherichia coli

<400>  47

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  48
<211>  1476
<212>  DNA
<213>  Methanococcus jannaschii


<220>
<221>  CDS
<222>  (1)..(1476)

<400>  48
atg atg gta agg ata ttt gat aca aca ctt aga gat gga gag caa aca         48
Met Met Val Arg Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu Gln Thr           
1               5                   10                  15                

cca gga gtt tct tta aca cca aat gat aag tta gag ata gca aaa aaa         96
Pro Gly Val Ser Leu Thr Pro Asn Asp Lys Leu Glu Ile Ala Lys Lys           
            20                  25                  30                    

ttg gat gag ctt gga gtt gat gtt ata gag gca ggt tca gct ata act        144
Leu Asp Glu Leu Gly Val Asp Val Ile Glu Ala Gly Ser Ala Ile Thr           
        35                  40                  45                        

tca aaa gga gag aga gaa gga ata aaa tta ata aca aaa gaa ggt tta        192
Ser Lys Gly Glu Arg Glu Gly Ile Lys Leu Ile Thr Lys Glu Gly Leu           
    50                  55                  60                            

aat gca gaa atc tgc tca ttt gtt aga gct tta cct gta gat att gat        240
Asn Ala Glu Ile Cys Ser Phe Val Arg Ala Leu Pro Val Asp Ile Asp           
65                  70                  75                  80            

gct gcc tta gaa tgt gat gta gat agt gtc cat tta gta gtg cca aca        288
Ala Ala Leu Glu Cys Asp Val Asp Ser Val His Leu Val Val Pro Thr           
                85                  90                  95                

tct cca ata cac atg aaa tat aag ctt aga aaa aca gaa gat gag gtt        336
Ser Pro Ile His Met Lys Tyr Lys Leu Arg Lys Thr Glu Asp Glu Val           
            100                 105                 110                   

tta gag aca gct tta aag gct gta gag tat gct aaa gaa cat gga ttg        384
Leu Glu Thr Ala Leu Lys Ala Val Glu Tyr Ala Lys Glu His Gly Leu           
        115                 120                 125                       

att gtt gag tta tct gca gag gat gca aca aga agt gat gta aat ttc        432
Ile Val Glu Leu Ser Ala Glu Asp Ala Thr Arg Ser Asp Val Asn Phe           
    130                 135                 140                           

tta ata aaa cta ttt aat gaa ggg gaa aag gtt gga gca gac aga gtt        480
Leu Ile Lys Leu Phe Asn Glu Gly Glu Lys Val Gly Ala Asp Arg Val           
145                 150                 155                 160           

tgt gtt tgt gac aca gta gga gtt tta act cca caa aag agt cag gaa        528
Cys Val Cys Asp Thr Val Gly Val Leu Thr Pro Gln Lys Ser Gln Glu           
                165                 170                 175               

tta ttt aaa aaa ata act gaa aat gtt aat tta ccg gtc tca gtt cat        576
Leu Phe Lys Lys Ile Thr Glu Asn Val Asn Leu Pro Val Ser Val His           
            180                 185                 190                   

tgc cac aac gac ttt gga atg gct act gct aat act tgc tca gca gtt        624
Cys His Asn Asp Phe Gly Met Ala Thr Ala Asn Thr Cys Ser Ala Val           
        195                 200                 205                       

tta ggt gga gct gtt cag tgc cac gta aca gtt aat ggt att gga gag        672
Leu Gly Gly Ala Val Gln Cys His Val Thr Val Asn Gly Ile Gly Glu           
    210                 215                 220                           

aga gca gga aat gcc tca ttg gaa gag gtt gtt gct gct tta aaa ata        720
Arg Ala Gly Asn Ala Ser Leu Glu Glu Val Val Ala Ala Leu Lys Ile           
225                 230                 235                 240           

ctc tat ggc tat gat act aag ata aag atg gaa aag tta tat gag gtt        768
Leu Tyr Gly Tyr Asp Thr Lys Ile Lys Met Glu Lys Leu Tyr Glu Val           
                245                 250                 255               

tca aga att gtc tca aga ttg atg aaa ctt cct gtt cca cca aat aaa        816
Ser Arg Ile Val Ser Arg Leu Met Lys Leu Pro Val Pro Pro Asn Lys           
            260                 265                 270                   

gca att gtt ggg gac aat gca ttt gct cat gaa gca gga ata cat gtt        864
Ala Ile Val Gly Asp Asn Ala Phe Ala His Glu Ala Gly Ile His Val           
        275                 280                 285                       

gat gga tta ata aaa aat act gaa acc tat gag cca ata aaa cca gaa        912
Asp Gly Leu Ile Lys Asn Thr Glu Thr Tyr Glu Pro Ile Lys Pro Glu           
    290                 295                 300                           

atg gtt ggg aat aga aga aga att att ttg ggt aag cat tct ggt aga        960
Met Val Gly Asn Arg Arg Arg Ile Ile Leu Gly Lys His Ser Gly Arg           
305                 310                 315                 320           

aaa gct tta aaa tac aaa ctt gat ttg atg ggc ata aac gtt agt gat       1008
Lys Ala Leu Lys Tyr Lys Leu Asp Leu Met Gly Ile Asn Val Ser Asp           
                325                 330                 335               

gag caa tta aat aaa ata tat gaa aga gtt aaa gaa ttt ggg gat ttg       1056
Glu Gln Leu Asn Lys Ile Tyr Glu Arg Val Lys Glu Phe Gly Asp Leu           
            340                 345                 350                   

ggt aaa tac att tca gac gct gat ttg ttg gct ata gtt aga gaa gtt       1104
Gly Lys Tyr Ile Ser Asp Ala Asp Leu Leu Ala Ile Val Arg Glu Val           
        355                 360                 365                       

act gga aaa ttg gta gaa gag aaa atc aaa tta gat gaa tta act gtt       1152
Thr Gly Lys Leu Val Glu Glu Lys Ile Lys Leu Asp Glu Leu Thr Val           
    370                 375                 380                           

gta tct gga aat aaa ata aca cca att gca tct gtt aaa ctc cat tat       1200
Val Ser Gly Asn Lys Ile Thr Pro Ile Ala Ser Val Lys Leu His Tyr           
385                 390                 395                 400           

aaa gga gaa gat ata act tta ata gaa act gct tat ggt gtt gga ccg       1248
Lys Gly Glu Asp Ile Thr Leu Ile Glu Thr Ala Tyr Gly Val Gly Pro           
                405                 410                 415               

gta gat gca gca ata aat gct gtg aga aag gca ata agt gga gtt gca       1296
Val Asp Ala Ala Ile Asn Ala Val Arg Lys Ala Ile Ser Gly Val Ala           
            420                 425                 430                   

gat att aag ttg gta gag tat aga gtt gaa gca att ggt gga gga act       1344
Asp Ile Lys Leu Val Glu Tyr Arg Val Glu Ala Ile Gly Gly Gly Thr           
        435                 440                 445                       

gat gcg tta ata gag gtt gtt gtt aaa tta aga aaa gga act gaa att       1392
Asp Ala Leu Ile Glu Val Val Val Lys Leu Arg Lys Gly Thr Glu Ile           
    450                 455                 460                           

gtt gaa gtt aga aaa tca gac gct gat ata ata agg gct tct gta gat       1440
Val Glu Val Arg Lys Ser Asp Ala Asp Ile Ile Arg Ala Ser Val Asp           
465                 470                 475                 480           

gct gta atg gaa gga atc aat atg tta ttg aat taa                       1476
Ala Val Met Glu Gly Ile Asn Met Leu Leu Asn                               
                485                 490                                   


<210>  49
<211>  491
<212>  PRT
<213>  Methanococcus jannaschii

<400>  49

Met Met Val Arg Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu Gln Thr 
1               5                   10                  15      


Pro Gly Val Ser Leu Thr Pro Asn Asp Lys Leu Glu Ile Ala Lys Lys 
            20                  25                  30          


Leu Asp Glu Leu Gly Val Asp Val Ile Glu Ala Gly Ser Ala Ile Thr 
        35                  40                  45              


Ser Lys Gly Glu Arg Glu Gly Ile Lys Leu Ile Thr Lys Glu Gly Leu 
    50                  55                  60                  


Asn Ala Glu Ile Cys Ser Phe Val Arg Ala Leu Pro Val Asp Ile Asp 
65                  70                  75                  80  


Ala Ala Leu Glu Cys Asp Val Asp Ser Val His Leu Val Val Pro Thr 
                85                  90                  95      


Ser Pro Ile His Met Lys Tyr Lys Leu Arg Lys Thr Glu Asp Glu Val 
            100                 105                 110         


Leu Glu Thr Ala Leu Lys Ala Val Glu Tyr Ala Lys Glu His Gly Leu 
        115                 120                 125             


Ile Val Glu Leu Ser Ala Glu Asp Ala Thr Arg Ser Asp Val Asn Phe 
    130                 135                 140                 


Leu Ile Lys Leu Phe Asn Glu Gly Glu Lys Val Gly Ala Asp Arg Val 
145                 150                 155                 160 


Cys Val Cys Asp Thr Val Gly Val Leu Thr Pro Gln Lys Ser Gln Glu 
                165                 170                 175     


Leu Phe Lys Lys Ile Thr Glu Asn Val Asn Leu Pro Val Ser Val His 
            180                 185                 190         


Cys His Asn Asp Phe Gly Met Ala Thr Ala Asn Thr Cys Ser Ala Val 
        195                 200                 205             


Leu Gly Gly Ala Val Gln Cys His Val Thr Val Asn Gly Ile Gly Glu 
    210                 215                 220                 


Arg Ala Gly Asn Ala Ser Leu Glu Glu Val Val Ala Ala Leu Lys Ile 
225                 230                 235                 240 


Leu Tyr Gly Tyr Asp Thr Lys Ile Lys Met Glu Lys Leu Tyr Glu Val 
                245                 250                 255     


Ser Arg Ile Val Ser Arg Leu Met Lys Leu Pro Val Pro Pro Asn Lys 
            260                 265                 270         


Ala Ile Val Gly Asp Asn Ala Phe Ala His Glu Ala Gly Ile His Val 
        275                 280                 285             


Asp Gly Leu Ile Lys Asn Thr Glu Thr Tyr Glu Pro Ile Lys Pro Glu 
    290                 295                 300                 


Met Val Gly Asn Arg Arg Arg Ile Ile Leu Gly Lys His Ser Gly Arg 
305                 310                 315                 320 


Lys Ala Leu Lys Tyr Lys Leu Asp Leu Met Gly Ile Asn Val Ser Asp 
                325                 330                 335     


Glu Gln Leu Asn Lys Ile Tyr Glu Arg Val Lys Glu Phe Gly Asp Leu 
            340                 345                 350         


Gly Lys Tyr Ile Ser Asp Ala Asp Leu Leu Ala Ile Val Arg Glu Val 
        355                 360                 365             


Thr Gly Lys Leu Val Glu Glu Lys Ile Lys Leu Asp Glu Leu Thr Val 
    370                 375                 380                 


Val Ser Gly Asn Lys Ile Thr Pro Ile Ala Ser Val Lys Leu His Tyr 
385                 390                 395                 400 


Lys Gly Glu Asp Ile Thr Leu Ile Glu Thr Ala Tyr Gly Val Gly Pro 
                405                 410                 415     


Val Asp Ala Ala Ile Asn Ala Val Arg Lys Ala Ile Ser Gly Val Ala 
            420                 425                 430         


Asp Ile Lys Leu Val Glu Tyr Arg Val Glu Ala Ile Gly Gly Gly Thr 
        435                 440                 445             


Asp Ala Leu Ile Glu Val Val Val Lys Leu Arg Lys Gly Thr Glu Ile 
    450                 455                 460                 


Val Glu Val Arg Lys Ser Asp Ala Asp Ile Ile Arg Ala Ser Val Asp 
465                 470                 475                 480 


Ala Val Met Glu Gly Ile Asn Met Leu Leu Asn 
                485                 490     


<210>  50
<211>  264
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(264)

<400>  50
atg atg caa cat cag gtc aat gta tcg gct cgc ttc aat cca gaa acc         48
Met Met Gln His Gln Val Asn Val Ser Ala Arg Phe Asn Pro Glu Thr           
1               5                   10                  15                

tta gaa cgt gtt tta cgc gtg gtg cgt cat cgt ggt ttc cac gtc tgc         96
Leu Glu Arg Val Leu Arg Val Val Arg His Arg Gly Phe His Val Cys           
            20                  25                  30                    

tca atg aat atg gcc gcc gcc agc gat gca caa aat ata aat atc gaa        144
Ser Met Asn Met Ala Ala Ala Ser Asp Ala Gln Asn Ile Asn Ile Glu           
        35                  40                  45                        

ttg acc gtt gcc agc cca cgg tcg gtc gac tta ctg ttt agt cag tta        192
Leu Thr Val Ala Ser Pro Arg Ser Val Asp Leu Leu Phe Ser Gln Leu           
    50                  55                  60                            

aat aaa ctg gtg gac gtc gca cac gtt gcc atc tgc cag agc aca acc        240
Asn Lys Leu Val Asp Val Ala His Val Ala Ile Cys Gln Ser Thr Thr           
65                  70                  75                  80            

aca tca caa caa atc cgc gcc tga                                        264
Thr Ser Gln Gln Ile Arg Ala                                               
                85                                                        


<210>  51
<211>  87
<212>  PRT
<213>  Escherichia coli

<400>  51

Met Met Gln His Gln Val Asn Val Ser Ala Arg Phe Asn Pro Glu Thr 
1               5                   10                  15      


Leu Glu Arg Val Leu Arg Val Val Arg His Arg Gly Phe His Val Cys 
            20                  25                  30          


Ser Met Asn Met Ala Ala Ala Ser Asp Ala Gln Asn Ile Asn Ile Glu 
        35                  40                  45              


Leu Thr Val Ala Ser Pro Arg Ser Val Asp Leu Leu Phe Ser Gln Leu 
    50                  55                  60                  


Asn Lys Leu Val Asp Val Ala His Val Ala Ile Cys Gln Ser Thr Thr 
65                  70                  75                  80  


Thr Ser Gln Gln Ile Arg Ala 
                85          


<210>  52
<211>  582
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(582)

<400>  52
ttg ttg tta aaa caa ctg tcg gat cgt aaa cct gcg gat tgc gtc gtg         48
Leu Leu Leu Lys Gln Leu Ser Asp Arg Lys Pro Ala Asp Cys Val Val           
1               5                   10                  15                

acc aca gat gtg ggg cag cac cag atg tgg gct gcg cag cac atc gcc         96
Thr Thr Asp Val Gly Gln His Gln Met Trp Ala Ala Gln His Ile Ala           
            20                  25                  30                    

cac act cgc ccg gaa aat ttc atc acc tcc agc ggt tta ggt acc atg        144
His Thr Arg Pro Glu Asn Phe Ile Thr Ser Ser Gly Leu Gly Thr Met           
        35                  40                  45                        

ggt ttt ggt tta ccg gcg gcg gtt ggc gca caa gtc gcg cga ccg aac        192
Gly Phe Gly Leu Pro Ala Ala Val Gly Ala Gln Val Ala Arg Pro Asn           
    50                  55                  60                            

gat acc gtt gtc tgt atc tcc ggt gac ggc tct ttc atg atg aat gtg        240
Asp Thr Val Val Cys Ile Ser Gly Asp Gly Ser Phe Met Met Asn Val           
65                  70                  75                  80            

caa gag ctg ggc acc gta aaa cgc aag cag tta ccg ttg aaa atc gtc        288
Gln Glu Leu Gly Thr Val Lys Arg Lys Gln Leu Pro Leu Lys Ile Val           
                85                  90                  95                

tta ctc gat aac caa cgg tta ggg atg gtt cga caa tgg cag caa ctg        336
Leu Leu Asp Asn Gln Arg Leu Gly Met Val Arg Gln Trp Gln Gln Leu           
            100                 105                 110                   

ttt ttt cag gaa cga tac agc gaa acc acc ctt act gat aac ccc gat        384
Phe Phe Gln Glu Arg Tyr Ser Glu Thr Thr Leu Thr Asp Asn Pro Asp           
        115                 120                 125                       

ttc ctc atg tta gcc agc gcc ttc ggc atc cat ggc caa cac atc acc        432
Phe Leu Met Leu Ala Ser Ala Phe Gly Ile His Gly Gln His Ile Thr           
    130                 135                 140                           

cgg aaa gac cag gtt gaa gcg gca ctc gac acc atg ctg aac agt gat        480
Arg Lys Asp Gln Val Glu Ala Ala Leu Asp Thr Met Leu Asn Ser Asp           
145                 150                 155                 160           

ggg cca tac ctg ctt cat gtc tca atc gac gaa ctt gag aac gtc tgg        528
Gly Pro Tyr Leu Leu His Val Ser Ile Asp Glu Leu Glu Asn Val Trp           
                165                 170                 175               

ccg ctg gtg ccg cct ggc gcc agt aat tca gaa atg ttg gag aaa tta        576
Pro Leu Val Pro Pro Gly Ala Ser Asn Ser Glu Met Leu Glu Lys Leu           
            180                 185                 190                   

tca tga                                                                582
Ser                                                                       
                                                                          


<210>  53
<211>  193
<212>  PRT
<213>  Escherichia coli

<400>  53

Leu Leu Leu Lys Gln Leu Ser Asp Arg Lys Pro Ala Asp Cys Val Val 
1               5                   10                  15      


Thr Thr Asp Val Gly Gln His Gln Met Trp Ala Ala Gln His Ile Ala 
            20                  25                  30          


His Thr Arg Pro Glu Asn Phe Ile Thr Ser Ser Gly Leu Gly Thr Met 
        35                  40                  45              


Gly Phe Gly Leu Pro Ala Ala Val Gly Ala Gln Val Ala Arg Pro Asn 
    50                  55                  60                  


Asp Thr Val Val Cys Ile Ser Gly Asp Gly Ser Phe Met Met Asn Val 
65                  70                  75                  80  


Gln Glu Leu Gly Thr Val Lys Arg Lys Gln Leu Pro Leu Lys Ile Val 
                85                  90                  95      


Leu Leu Asp Asn Gln Arg Leu Gly Met Val Arg Gln Trp Gln Gln Leu 
            100                 105                 110         


Phe Phe Gln Glu Arg Tyr Ser Glu Thr Thr Leu Thr Asp Asn Pro Asp 
        115                 120                 125             


Phe Leu Met Leu Ala Ser Ala Phe Gly Ile His Gly Gln His Ile Thr 
    130                 135                 140                 


Arg Lys Asp Gln Val Glu Ala Ala Leu Asp Thr Met Leu Asn Ser Asp 
145                 150                 155                 160 


Gly Pro Tyr Leu Leu His Val Ser Ile Asp Glu Leu Glu Asn Val Trp 
                165                 170                 175     


Pro Leu Val Pro Pro Gly Ala Ser Asn Ser Glu Met Leu Glu Lys Leu 
            180                 185                 190         


Ser 
    


<210>  54
<211>  291
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(291)

<400>  54
atg caa aac aca act cat gac aac gta att ctg gag ctc acc gtt cgc         48
Met Gln Asn Thr Thr His Asp Asn Val Ile Leu Glu Leu Thr Val Arg           
1               5                   10                  15                

aac cat ccg ggc gta atg acc cac gtt tgt ggc ctt ttt gcc cgc cgc         96
Asn His Pro Gly Val Met Thr His Val Cys Gly Leu Phe Ala Arg Arg           
            20                  25                  30                    

gct ttt aac gtt gaa ggc att ctt tgt ctg ccg att cag gac agc gac        144
Ala Phe Asn Val Glu Gly Ile Leu Cys Leu Pro Ile Gln Asp Ser Asp           
        35                  40                  45                        

aaa agc cat atc tgg cta ctg gtc aat gac gac cag cgt ctg gag cag        192
Lys Ser His Ile Trp Leu Leu Val Asn Asp Asp Gln Arg Leu Glu Gln           
    50                  55                  60                            

atg ata agc caa atc gat aag ctg gaa gat gtc gtg aaa gtg cag cgt        240
Met Ile Ser Gln Ile Asp Lys Leu Glu Asp Val Val Lys Val Gln Arg           
65                  70                  75                  80            

aat cag tcc gat ccg acg atg ttt aac aag atc gcg gtg ttt ttt cag        288
Asn Gln Ser Asp Pro Thr Met Phe Asn Lys Ile Ala Val Phe Phe Gln           
                85                  90                  95                

taa                                                                    291


<210>  55
<211>  96
<212>  PRT
<213>  Escherichia coli

<400>  55

Met Gln Asn Thr Thr His Asp Asn Val Ile Leu Glu Leu Thr Val Arg 
1               5                   10                  15      


Asn His Pro Gly Val Met Thr His Val Cys Gly Leu Phe Ala Arg Arg 
            20                  25                  30          


Ala Phe Asn Val Glu Gly Ile Leu Cys Leu Pro Ile Gln Asp Ser Asp 
        35                  40                  45              


Lys Ser His Ile Trp Leu Leu Val Asn Asp Asp Gln Arg Leu Glu Gln 
    50                  55                  60                  


Met Ile Ser Gln Ile Asp Lys Leu Glu Asp Val Val Lys Val Gln Arg 
65                  70                  75                  80  


Asn Gln Ser Asp Pro Thr Met Phe Asn Lys Ile Ala Val Phe Phe Gln 
                85                  90                  95      


<210>  56
<211>  1689
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1689)

<400>  56
atg gca agt tcg ggc aca aca tcg acg cgt aag cgc ttt acc ggc gca         48
Met Ala Ser Ser Gly Thr Thr Ser Thr Arg Lys Arg Phe Thr Gly Ala           
1               5                   10                  15                

gaa ttt atc gtt cat ttc ctg gaa cag cag ggc att aag att gtg aca         96
Glu Phe Ile Val His Phe Leu Glu Gln Gln Gly Ile Lys Ile Val Thr           
            20                  25                  30                    

ggc att ccg ggc ggt tct atc ctg cct gtt tac gat gcc tta agc caa        144
Gly Ile Pro Gly Gly Ser Ile Leu Pro Val Tyr Asp Ala Leu Ser Gln           
        35                  40                  45                        

agc acg caa atc cgc cat att ctg gcc cgt cat gaa cag ggc gcg ggc        192
Ser Thr Gln Ile Arg His Ile Leu Ala Arg His Glu Gln Gly Ala Gly           
    50                  55                  60                            

ttt atc gct cag gga atg gcg cgc acc gac ggt aaa ccg gcg gtc tgt        240
Phe Ile Ala Gln Gly Met Ala Arg Thr Asp Gly Lys Pro Ala Val Cys           
65                  70                  75                  80            

atg gcc tgt agc gga ccg ggt gcg act aac ctg gtg acc gcc att gcc        288
Met Ala Cys Ser Gly Pro Gly Ala Thr Asn Leu Val Thr Ala Ile Ala           
                85                  90                  95                

gat gcg cgg ctg gac tcc atc ccg ctg att tgc atc act ggt cag gtt        336
Asp Ala Arg Leu Asp Ser Ile Pro Leu Ile Cys Ile Thr Gly Gln Val           
            100                 105                 110                   

ccc gcc tcg atg atc ggc acc gac gcc ttc cag gaa gtg gac acc tac        384
Pro Ala Ser Met Ile Gly Thr Asp Ala Phe Gln Glu Val Asp Thr Tyr           
        115                 120                 125                       

ggc atc tct atc ccc atc acc aaa cac aac tat ctg gtc aga cat atc        432
Gly Ile Ser Ile Pro Ile Thr Lys His Asn Tyr Leu Val Arg His Ile           
    130                 135                 140                           

gaa gaa ctc ccg cag gtc atg agc gat gcc ttc cgc att gcg caa tca        480
Glu Glu Leu Pro Gln Val Met Ser Asp Ala Phe Arg Ile Ala Gln Ser           
145                 150                 155                 160           

ggc cgc cca ggc ccg gtg tgg ata gac att cct aag gat gtg caa acg        528
Gly Arg Pro Gly Pro Val Trp Ile Asp Ile Pro Lys Asp Val Gln Thr           
                165                 170                 175               

gca gtt ttt gag att gaa aca cag ccc gct atg gca gaa aaa gcc gcc        576
Ala Val Phe Glu Ile Glu Thr Gln Pro Ala Met Ala Glu Lys Ala Ala           
            180                 185                 190                   

gcc ccc gcc ttt agc gaa gaa agc att cgt gac gca gcg gcg atg att        624
Ala Pro Ala Phe Ser Glu Glu Ser Ile Arg Asp Ala Ala Ala Met Ile           
        195                 200                 205                       

aac gct gcc aaa cgc ccg gtg ctt tat ctg ggc ggc ggt gtg atc aat        672
Asn Ala Ala Lys Arg Pro Val Leu Tyr Leu Gly Gly Gly Val Ile Asn           
    210                 215                 220                           

gcg ccc gca cgg gtg cgt gaa ctg gcg gag aaa gcg caa ctg cct acc        720
Ala Pro Ala Arg Val Arg Glu Leu Ala Glu Lys Ala Gln Leu Pro Thr           
225                 230                 235                 240           

acc atg act tta atg gcg ctg ggc atg ttg cca aaa gcg cat ccg ttg        768
Thr Met Thr Leu Met Ala Leu Gly Met Leu Pro Lys Ala His Pro Leu           
                245                 250                 255               

tcg ctg ggt atg ctg ggg atg cac ggc gtg cgc agc acc aac tat att        816
Ser Leu Gly Met Leu Gly Met His Gly Val Arg Ser Thr Asn Tyr Ile           
            260                 265                 270                   

ttg cag gag gcg gat ttg ttg ata gtg ctc ggt gcg cgt ttt gat gac        864
Leu Gln Glu Ala Asp Leu Leu Ile Val Leu Gly Ala Arg Phe Asp Asp           
        275                 280                 285                       

cgg gcg att ggc aaa acc gag cag ttc tgt ccg aat gcc aaa atc att        912
Arg Ala Ile Gly Lys Thr Glu Gln Phe Cys Pro Asn Ala Lys Ile Ile           
    290                 295                 300                           

cat gtc gat atc gac cgt gca gag ctg ggt aaa atc aag cag ccg cac        960
His Val Asp Ile Asp Arg Ala Glu Leu Gly Lys Ile Lys Gln Pro His           
305                 310                 315                 320           

gtg gcg att cag gcg gat gtt gat gac gtg ctg gcg cag ttg atc ccg       1008
Val Ala Ile Gln Ala Asp Val Asp Asp Val Leu Ala Gln Leu Ile Pro           
                325                 330                 335               

ctg gtg gaa gcg caa ccg cgt gca gag tgg cac cag ttg gta gcg gat       1056
Leu Val Glu Ala Gln Pro Arg Ala Glu Trp His Gln Leu Val Ala Asp           
            340                 345                 350                   

ttg cag cgt gag ttt ccg tgt cca atc ccg aaa gcg tgc gat ccg tta       1104
Leu Gln Arg Glu Phe Pro Cys Pro Ile Pro Lys Ala Cys Asp Pro Leu           
        355                 360                 365                       

agc cat tac ggc ctg atc aac gcc gtt gcc gcc tgt gtc gat gac aat       1152
Ser His Tyr Gly Leu Ile Asn Ala Val Ala Ala Cys Val Asp Asp Asn           
    370                 375                 380                           

gca att atc acc acc gac gtt ggt cag cat cag atg tgg acc gcg caa       1200
Ala Ile Ile Thr Thr Asp Val Gly Gln His Gln Met Trp Thr Ala Gln           
385                 390                 395                 400           

gct tat ccg ctc aat cgc cca cgc cag tgg ctg acc tcc ggt ggg ctg       1248
Ala Tyr Pro Leu Asn Arg Pro Arg Gln Trp Leu Thr Ser Gly Gly Leu           
                405                 410                 415               

ggc acg atg ggt ttt ggc ctg cct gcg gcg att ggc gct gcg ctg gcg       1296
Gly Thr Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala Leu Ala           
            420                 425                 430                   

aac ccg gat cgc aaa gtg ttg tgt ttc tcc ggc gac ggc agc ctg atg       1344
Asn Pro Asp Arg Lys Val Leu Cys Phe Ser Gly Asp Gly Ser Leu Met           
        435                 440                 445                       

atg aat att cag gag atg gcg acc gcc agt gaa aat cag ctg gat gtc       1392
Met Asn Ile Gln Glu Met Ala Thr Ala Ser Glu Asn Gln Leu Asp Val           
    450                 455                 460                           

aaa atc att ctg atg aac aac gaa gcg ctg ggg ctg gtg cat cag caa       1440
Lys Ile Ile Leu Met Asn Asn Glu Ala Leu Gly Leu Val His Gln Gln           
465                 470                 475                 480           

cag agt ctg ttc tac gag caa ggc gtt ttt gcc gcc acc tat ccg ggc       1488
Gln Ser Leu Phe Tyr Glu Gln Gly Val Phe Ala Ala Thr Tyr Pro Gly           
                485                 490                 495               

aaa atc aac ttt atg cag att gcc gcc gga ttc ggc ctc gaa acc tgt       1536
Lys Ile Asn Phe Met Gln Ile Ala Ala Gly Phe Gly Leu Glu Thr Cys           
            500                 505                 510                   

gat ttg aat aac gaa gcc gat ccg cag gct tca ttg cag gaa atc atc       1584
Asp Leu Asn Asn Glu Ala Asp Pro Gln Ala Ser Leu Gln Glu Ile Ile           
        515                 520                 525                       

aat cgc cct ggc ccg gcg ctg atc cat gtg cgc att gat gcc gaa gaa       1632
Asn Arg Pro Gly Pro Ala Leu Ile His Val Arg Ile Asp Ala Glu Glu           
    530                 535                 540                           

aaa gtt tac ccg atg gtg ccg cca ggt gcg gcg aat act gaa atg gtg       1680
Lys Val Tyr Pro Met Val Pro Pro Gly Ala Ala Asn Thr Glu Met Val           
545                 550                 555                 560           

ggg gaa taa                                                           1689
Gly Glu                                                                   
                                                                          


<210>  57
<211>  562
<212>  PRT
<213>  Escherichia coli

<400>  57

Met Ala Ser Ser Gly Thr Thr Ser Thr Arg Lys Arg Phe Thr Gly Ala 
1               5                   10                  15      


Glu Phe Ile Val His Phe Leu Glu Gln Gln Gly Ile Lys Ile Val Thr 
            20                  25                  30          


Gly Ile Pro Gly Gly Ser Ile Leu Pro Val Tyr Asp Ala Leu Ser Gln 
        35                  40                  45              


Ser Thr Gln Ile Arg His Ile Leu Ala Arg His Glu Gln Gly Ala Gly 
    50                  55                  60                  


Phe Ile Ala Gln Gly Met Ala Arg Thr Asp Gly Lys Pro Ala Val Cys 
65                  70                  75                  80  


Met Ala Cys Ser Gly Pro Gly Ala Thr Asn Leu Val Thr Ala Ile Ala 
                85                  90                  95      


Asp Ala Arg Leu Asp Ser Ile Pro Leu Ile Cys Ile Thr Gly Gln Val 
            100                 105                 110         


Pro Ala Ser Met Ile Gly Thr Asp Ala Phe Gln Glu Val Asp Thr Tyr 
        115                 120                 125             


Gly Ile Ser Ile Pro Ile Thr Lys His Asn Tyr Leu Val Arg His Ile 
    130                 135                 140                 


Glu Glu Leu Pro Gln Val Met Ser Asp Ala Phe Arg Ile Ala Gln Ser 
145                 150                 155                 160 


Gly Arg Pro Gly Pro Val Trp Ile Asp Ile Pro Lys Asp Val Gln Thr 
                165                 170                 175     


Ala Val Phe Glu Ile Glu Thr Gln Pro Ala Met Ala Glu Lys Ala Ala 
            180                 185                 190         


Ala Pro Ala Phe Ser Glu Glu Ser Ile Arg Asp Ala Ala Ala Met Ile 
        195                 200                 205             


Asn Ala Ala Lys Arg Pro Val Leu Tyr Leu Gly Gly Gly Val Ile Asn 
    210                 215                 220                 


Ala Pro Ala Arg Val Arg Glu Leu Ala Glu Lys Ala Gln Leu Pro Thr 
225                 230                 235                 240 


Thr Met Thr Leu Met Ala Leu Gly Met Leu Pro Lys Ala His Pro Leu 
                245                 250                 255     


Ser Leu Gly Met Leu Gly Met His Gly Val Arg Ser Thr Asn Tyr Ile 
            260                 265                 270         


Leu Gln Glu Ala Asp Leu Leu Ile Val Leu Gly Ala Arg Phe Asp Asp 
        275                 280                 285             


Arg Ala Ile Gly Lys Thr Glu Gln Phe Cys Pro Asn Ala Lys Ile Ile 
    290                 295                 300                 


His Val Asp Ile Asp Arg Ala Glu Leu Gly Lys Ile Lys Gln Pro His 
305                 310                 315                 320 


Val Ala Ile Gln Ala Asp Val Asp Asp Val Leu Ala Gln Leu Ile Pro 
                325                 330                 335     


Leu Val Glu Ala Gln Pro Arg Ala Glu Trp His Gln Leu Val Ala Asp 
            340                 345                 350         


Leu Gln Arg Glu Phe Pro Cys Pro Ile Pro Lys Ala Cys Asp Pro Leu 
        355                 360                 365             


Ser His Tyr Gly Leu Ile Asn Ala Val Ala Ala Cys Val Asp Asp Asn 
    370                 375                 380                 


Ala Ile Ile Thr Thr Asp Val Gly Gln His Gln Met Trp Thr Ala Gln 
385                 390                 395                 400 


Ala Tyr Pro Leu Asn Arg Pro Arg Gln Trp Leu Thr Ser Gly Gly Leu 
                405                 410                 415     


Gly Thr Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala Leu Ala 
            420                 425                 430         


Asn Pro Asp Arg Lys Val Leu Cys Phe Ser Gly Asp Gly Ser Leu Met 
        435                 440                 445             


Met Asn Ile Gln Glu Met Ala Thr Ala Ser Glu Asn Gln Leu Asp Val 
    450                 455                 460                 


Lys Ile Ile Leu Met Asn Asn Glu Ala Leu Gly Leu Val His Gln Gln 
465                 470                 475                 480 


Gln Ser Leu Phe Tyr Glu Gln Gly Val Phe Ala Ala Thr Tyr Pro Gly 
                485                 490                 495     


Lys Ile Asn Phe Met Gln Ile Ala Ala Gly Phe Gly Leu Glu Thr Cys 
            500                 505                 510         


Asp Leu Asn Asn Glu Ala Asp Pro Gln Ala Ser Leu Gln Glu Ile Ile 
        515                 520                 525             


Asn Arg Pro Gly Pro Ala Leu Ile His Val Arg Ile Asp Ala Glu Glu 
    530                 535                 540                 


Lys Val Tyr Pro Met Val Pro Pro Gly Ala Ala Asn Thr Glu Met Val 
545                 550                 555                 560 


Gly Glu 
        


<210>  58
<211>  2577
<212>  DNA
<213>  Clostridium acetobutylicum


<220>
<221>  CDS
<222>  (1)..(2577)

<400>  58
atg aaa gtt aca aat caa aaa gaa cta aaa caa aag cta aat gaa ttg         48
Met Lys Val Thr Asn Gln Lys Glu Leu Lys Gln Lys Leu Asn Glu Leu           
1               5                   10                  15                

aga gaa gcg caa aag aag ttt gca acc tat act caa gag caa gtt gat         96
Arg Glu Ala Gln Lys Lys Phe Ala Thr Tyr Thr Gln Glu Gln Val Asp           
            20                  25                  30                    

aaa att ttt aaa caa tgt gcc ata gcc gca gct aaa gaa aga ata aac        144
Lys Ile Phe Lys Gln Cys Ala Ile Ala Ala Ala Lys Glu Arg Ile Asn           
        35                  40                  45                        

tta gct aaa tta gca gta gaa gaa aca gga ata ggt ctt gta gaa gat        192
Leu Ala Lys Leu Ala Val Glu Glu Thr Gly Ile Gly Leu Val Glu Asp           
    50                  55                  60                            

aaa att ata aaa aat cat ttt gca gca gaa tat ata tac aat aaa tat        240
Lys Ile Ile Lys Asn His Phe Ala Ala Glu Tyr Ile Tyr Asn Lys Tyr           
65                  70                  75                  80            

aaa aat gaa aaa act tgt ggc ata ata gac cat gac gat tct tta ggc        288
Lys Asn Glu Lys Thr Cys Gly Ile Ile Asp His Asp Asp Ser Leu Gly           
                85                  90                  95                

ata aca aag gtt gct gaa cca att gga att gtt gca gcc ata gtt cct        336
Ile Thr Lys Val Ala Glu Pro Ile Gly Ile Val Ala Ala Ile Val Pro           
            100                 105                 110                   

act act aat cca act tcc aca gca att ttc aaa tca tta att tct tta        384
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu           
        115                 120                 125                       

aaa aca aga aac gca ata ttc ttt tca cca cat cca cgt gca aaa aaa        432
Lys Thr Arg Asn Ala Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys           
    130                 135                 140                           

tct aca att gct gca gca aaa tta att tta gat gca gct gtt aaa gca        480
Ser Thr Ile Ala Ala Ala Lys Leu Ile Leu Asp Ala Ala Val Lys Ala           
145                 150                 155                 160           

gga gca cct aaa aat ata ata ggc tgg ata gat gag cca tca ata gaa        528
Gly Ala Pro Lys Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu           
                165                 170                 175               

ctt tct caa gat ttg atg agt gaa gct gat ata ata tta gca aca gga        576
Leu Ser Gln Asp Leu Met Ser Glu Ala Asp Ile Ile Leu Ala Thr Gly           
            180                 185                 190                   

ggt cct tca atg gtt aaa gcg gcc tat tca tct gga aaa cct gca att        624
Gly Pro Ser Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile           
        195                 200                 205                       

ggt gtt gga gca gga aat aca cca gca ata ata gat gag agt gca gat        672
Gly Val Gly Ala Gly Asn Thr Pro Ala Ile Ile Asp Glu Ser Ala Asp           
    210                 215                 220                           

ata gat atg gca gta agc tcc ata att tta tca aag act tat gac aat        720
Ile Asp Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn           
225                 230                 235                 240           

gga gta ata tgc gct tct gaa caa tca ata tta gtt atg aat tca ata        768
Gly Val Ile Cys Ala Ser Glu Gln Ser Ile Leu Val Met Asn Ser Ile           
                245                 250                 255               

tac gaa aaa gtt aaa gag gaa ttt gta aaa cga gga tca tat ata ctc        816
Tyr Glu Lys Val Lys Glu Glu Phe Val Lys Arg Gly Ser Tyr Ile Leu           
            260                 265                 270                   

aat caa aat gaa ata gct aaa ata aaa gaa act atg ttt aaa aat gga        864
Asn Gln Asn Glu Ile Ala Lys Ile Lys Glu Thr Met Phe Lys Asn Gly           
        275                 280                 285                       

gct att aat gct gac ata gtt gga aaa tct gct tat ata att gct aaa        912
Ala Ile Asn Ala Asp Ile Val Gly Lys Ser Ala Tyr Ile Ile Ala Lys           
    290                 295                 300                           

atg gca gga att gaa gtt cct caa act aca aag ata ctt ata ggc gaa        960
Met Ala Gly Ile Glu Val Pro Gln Thr Thr Lys Ile Leu Ile Gly Glu           
305                 310                 315                 320           

gta caa tct gtt gaa aaa agc gag ctg ttc tca cat gaa aaa cta tca       1008
Val Gln Ser Val Glu Lys Ser Glu Leu Phe Ser His Glu Lys Leu Ser           
                325                 330                 335               

cca gta ctt gca atg tat aaa gtt aag gat ttt gat gaa gct cta aaa       1056
Pro Val Leu Ala Met Tyr Lys Val Lys Asp Phe Asp Glu Ala Leu Lys           
            340                 345                 350                   

aag gca caa agg cta ata gaa tta ggt gga agt gga cac acg tca tct       1104
Lys Ala Gln Arg Leu Ile Glu Leu Gly Gly Ser Gly His Thr Ser Ser           
        355                 360                 365                       

tta tat ata gat tca caa aac aat aag gat aaa gtt aaa gaa ttt gga       1152
Leu Tyr Ile Asp Ser Gln Asn Asn Lys Asp Lys Val Lys Glu Phe Gly           
    370                 375                 380                           

tta gca atg aaa act tca agg aca ttt att aac atg cct tct tca cag       1200
Leu Ala Met Lys Thr Ser Arg Thr Phe Ile Asn Met Pro Ser Ser Gln           
385                 390                 395                 400           

gga gca agc gga gat tta tac aat ttt gcg ata gca cca tca ttt act       1248
Gly Ala Ser Gly Asp Leu Tyr Asn Phe Ala Ile Ala Pro Ser Phe Thr           
                405                 410                 415               

ctt gga tgc ggc act tgg gga gga aac tct gta tcg caa aat gta gag       1296
Leu Gly Cys Gly Thr Trp Gly Gly Asn Ser Val Ser Gln Asn Val Glu           
            420                 425                 430                   

cct aaa cat tta tta aat att aaa agt gtt gct gaa aga agg gaa aat       1344
Pro Lys His Leu Leu Asn Ile Lys Ser Val Ala Glu Arg Arg Glu Asn           
        435                 440                 445                       

atg ctt tgg ttt aaa gtg cca caa aaa ata tat ttt aaa tat gga tgt       1392
Met Leu Trp Phe Lys Val Pro Gln Lys Ile Tyr Phe Lys Tyr Gly Cys           
    450                 455                 460                           

ctt aga ttt gca tta aaa gaa tta aaa gat atg aat aag aaa aga gcc       1440
Leu Arg Phe Ala Leu Lys Glu Leu Lys Asp Met Asn Lys Lys Arg Ala           
465                 470                 475                 480           

ttt ata gta aca gat aaa gat ctt ttt aaa ctt gga tat gtt aat aaa       1488
Phe Ile Val Thr Asp Lys Asp Leu Phe Lys Leu Gly Tyr Val Asn Lys           
                485                 490                 495               

ata aca aag gta cta gat gag ata gat att aaa tac agt ata ttt aca       1536
Ile Thr Lys Val Leu Asp Glu Ile Asp Ile Lys Tyr Ser Ile Phe Thr           
            500                 505                 510                   

gat att aaa tct gat cca act att gat tca gta aaa aaa ggt gct aaa       1584
Asp Ile Lys Ser Asp Pro Thr Ile Asp Ser Val Lys Lys Gly Ala Lys           
        515                 520                 525                       

gaa atg ctt aac ttt gaa cct gat act ata atc tct att ggt ggt gga       1632
Glu Met Leu Asn Phe Glu Pro Asp Thr Ile Ile Ser Ile Gly Gly Gly           
    530                 535                 540                           

tcg cca atg gat gca gca aag gtt atg cac ttg tta tat gaa tat cca       1680
Ser Pro Met Asp Ala Ala Lys Val Met His Leu Leu Tyr Glu Tyr Pro           
545                 550                 555                 560           

gaa gca gaa att gaa aat cta gct ata aac ttt atg gat ata aga aag       1728
Glu Ala Glu Ile Glu Asn Leu Ala Ile Asn Phe Met Asp Ile Arg Lys           
                565                 570                 575               

aga ata tgc aat ttc cct aaa tta ggt aca aag gcg att tca gta gct       1776
Arg Ile Cys Asn Phe Pro Lys Leu Gly Thr Lys Ala Ile Ser Val Ala           
            580                 585                 590                   

att cct aca act gct ggt acc ggt tca gag gca aca cct ttt gca gtt       1824
Ile Pro Thr Thr Ala Gly Thr Gly Ser Glu Ala Thr Pro Phe Ala Val           
        595                 600                 605                       

ata act aat gat gaa aca gga atg aaa tac cct tta act tct tat gaa       1872
Ile Thr Asn Asp Glu Thr Gly Met Lys Tyr Pro Leu Thr Ser Tyr Glu           
    610                 615                 620                           

ttg acc cca aac atg gca ata ata gat act gaa tta atg tta aat atg       1920
Leu Thr Pro Asn Met Ala Ile Ile Asp Thr Glu Leu Met Leu Asn Met           
625                 630                 635                 640           

cct aga aaa tta aca gca gca act gga ata gat gca tta gtt cat gct       1968
Pro Arg Lys Leu Thr Ala Ala Thr Gly Ile Asp Ala Leu Val His Ala           
                645                 650                 655               

ata gaa gca tat gtt tcg gtt atg gct acg gat tat act gat gaa tta       2016
Ile Glu Ala Tyr Val Ser Val Met Ala Thr Asp Tyr Thr Asp Glu Leu           
            660                 665                 670                   

gcc tta aga gca ata aaa atg ata ttt aaa tat ttg cct aga gcc tat       2064
Ala Leu Arg Ala Ile Lys Met Ile Phe Lys Tyr Leu Pro Arg Ala Tyr           
        675                 680                 685                       

aaa aat ggg act aac gac att gaa gca aga gaa aaa atg gca cat gcc       2112
Lys Asn Gly Thr Asn Asp Ile Glu Ala Arg Glu Lys Met Ala His Ala           
    690                 695                 700                           

tct aat att gcg ggg atg gca ttt gca aat gct ttc tta ggt gta tgc       2160
Ser Asn Ile Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Val Cys           
705                 710                 715                 720           

cat tca atg gct cat aaa ctt ggg gca atg cat cac gtt cca cat gga       2208
His Ser Met Ala His Lys Leu Gly Ala Met His His Val Pro His Gly           
                725                 730                 735               

att gct tgt gct gta tta ata gaa gaa gtt att aaa tat aac gct aca       2256
Ile Ala Cys Ala Val Leu Ile Glu Glu Val Ile Lys Tyr Asn Ala Thr           
            740                 745                 750                   

gac tgt cca aca aag caa aca gca ttc cct caa tat aaa tct cct aat       2304
Asp Cys Pro Thr Lys Gln Thr Ala Phe Pro Gln Tyr Lys Ser Pro Asn           
        755                 760                 765                       

gct aag aga aaa tat gct gaa att gca gag tat ttg aat tta aag ggt       2352
Ala Lys Arg Lys Tyr Ala Glu Ile Ala Glu Tyr Leu Asn Leu Lys Gly           
    770                 775                 780                           

act agc gat acc gaa aag gta aca gcc tta ata gaa gct att tca aag       2400
Thr Ser Asp Thr Glu Lys Val Thr Ala Leu Ile Glu Ala Ile Ser Lys           
785                 790                 795                 800           

tta aag ata gat ttg agt att cca caa aat ata agt gcc gct gga ata       2448
Leu Lys Ile Asp Leu Ser Ile Pro Gln Asn Ile Ser Ala Ala Gly Ile           
                805                 810                 815               

aat aaa aaa gat ttt tat aat acg cta gat aaa atg tca gag ctt gct       2496
Asn Lys Lys Asp Phe Tyr Asn Thr Leu Asp Lys Met Ser Glu Leu Ala           
            820                 825                 830                   

ttt gat gac caa tgt aca aca gct aat cct agg tat cca ctt ata agt       2544
Phe Asp Asp Gln Cys Thr Thr Ala Asn Pro Arg Tyr Pro Leu Ile Ser           
        835                 840                 845                       

gaa ctt aag gat atc tat ata aaa tca ttt taa                           2577
Glu Leu Lys Asp Ile Tyr Ile Lys Ser Phe                                   
    850                 855                                               


<210>  59
<211>  858
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  59

Met Lys Val Thr Asn Gln Lys Glu Leu Lys Gln Lys Leu Asn Glu Leu 
1               5                   10                  15      


Arg Glu Ala Gln Lys Lys Phe Ala Thr Tyr Thr Gln Glu Gln Val Asp 
            20                  25                  30          


Lys Ile Phe Lys Gln Cys Ala Ile Ala Ala Ala Lys Glu Arg Ile Asn 
        35                  40                  45              


Leu Ala Lys Leu Ala Val Glu Glu Thr Gly Ile Gly Leu Val Glu Asp 
    50                  55                  60                  


Lys Ile Ile Lys Asn His Phe Ala Ala Glu Tyr Ile Tyr Asn Lys Tyr 
65                  70                  75                  80  


Lys Asn Glu Lys Thr Cys Gly Ile Ile Asp His Asp Asp Ser Leu Gly 
                85                  90                  95      


Ile Thr Lys Val Ala Glu Pro Ile Gly Ile Val Ala Ala Ile Val Pro 
            100                 105                 110         


Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu 
        115                 120                 125             


Lys Thr Arg Asn Ala Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys 
    130                 135                 140                 


Ser Thr Ile Ala Ala Ala Lys Leu Ile Leu Asp Ala Ala Val Lys Ala 
145                 150                 155                 160 


Gly Ala Pro Lys Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu 
                165                 170                 175     


Leu Ser Gln Asp Leu Met Ser Glu Ala Asp Ile Ile Leu Ala Thr Gly 
            180                 185                 190         


Gly Pro Ser Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile 
        195                 200                 205             


Gly Val Gly Ala Gly Asn Thr Pro Ala Ile Ile Asp Glu Ser Ala Asp 
    210                 215                 220                 


Ile Asp Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn 
225                 230                 235                 240 


Gly Val Ile Cys Ala Ser Glu Gln Ser Ile Leu Val Met Asn Ser Ile 
                245                 250                 255     


Tyr Glu Lys Val Lys Glu Glu Phe Val Lys Arg Gly Ser Tyr Ile Leu 
            260                 265                 270         


Asn Gln Asn Glu Ile Ala Lys Ile Lys Glu Thr Met Phe Lys Asn Gly 
        275                 280                 285             


Ala Ile Asn Ala Asp Ile Val Gly Lys Ser Ala Tyr Ile Ile Ala Lys 
    290                 295                 300                 


Met Ala Gly Ile Glu Val Pro Gln Thr Thr Lys Ile Leu Ile Gly Glu 
305                 310                 315                 320 


Val Gln Ser Val Glu Lys Ser Glu Leu Phe Ser His Glu Lys Leu Ser 
                325                 330                 335     


Pro Val Leu Ala Met Tyr Lys Val Lys Asp Phe Asp Glu Ala Leu Lys 
            340                 345                 350         


Lys Ala Gln Arg Leu Ile Glu Leu Gly Gly Ser Gly His Thr Ser Ser 
        355                 360                 365             


Leu Tyr Ile Asp Ser Gln Asn Asn Lys Asp Lys Val Lys Glu Phe Gly 
    370                 375                 380                 


Leu Ala Met Lys Thr Ser Arg Thr Phe Ile Asn Met Pro Ser Ser Gln 
385                 390                 395                 400 


Gly Ala Ser Gly Asp Leu Tyr Asn Phe Ala Ile Ala Pro Ser Phe Thr 
                405                 410                 415     


Leu Gly Cys Gly Thr Trp Gly Gly Asn Ser Val Ser Gln Asn Val Glu 
            420                 425                 430         


Pro Lys His Leu Leu Asn Ile Lys Ser Val Ala Glu Arg Arg Glu Asn 
        435                 440                 445             


Met Leu Trp Phe Lys Val Pro Gln Lys Ile Tyr Phe Lys Tyr Gly Cys 
    450                 455                 460                 


Leu Arg Phe Ala Leu Lys Glu Leu Lys Asp Met Asn Lys Lys Arg Ala 
465                 470                 475                 480 


Phe Ile Val Thr Asp Lys Asp Leu Phe Lys Leu Gly Tyr Val Asn Lys 
                485                 490                 495     


Ile Thr Lys Val Leu Asp Glu Ile Asp Ile Lys Tyr Ser Ile Phe Thr 
            500                 505                 510         


Asp Ile Lys Ser Asp Pro Thr Ile Asp Ser Val Lys Lys Gly Ala Lys 
        515                 520                 525             


Glu Met Leu Asn Phe Glu Pro Asp Thr Ile Ile Ser Ile Gly Gly Gly 
    530                 535                 540                 


Ser Pro Met Asp Ala Ala Lys Val Met His Leu Leu Tyr Glu Tyr Pro 
545                 550                 555                 560 


Glu Ala Glu Ile Glu Asn Leu Ala Ile Asn Phe Met Asp Ile Arg Lys 
                565                 570                 575     


Arg Ile Cys Asn Phe Pro Lys Leu Gly Thr Lys Ala Ile Ser Val Ala 
            580                 585                 590         


Ile Pro Thr Thr Ala Gly Thr Gly Ser Glu Ala Thr Pro Phe Ala Val 
        595                 600                 605             


Ile Thr Asn Asp Glu Thr Gly Met Lys Tyr Pro Leu Thr Ser Tyr Glu 
    610                 615                 620                 


Leu Thr Pro Asn Met Ala Ile Ile Asp Thr Glu Leu Met Leu Asn Met 
625                 630                 635                 640 


Pro Arg Lys Leu Thr Ala Ala Thr Gly Ile Asp Ala Leu Val His Ala 
                645                 650                 655     


Ile Glu Ala Tyr Val Ser Val Met Ala Thr Asp Tyr Thr Asp Glu Leu 
            660                 665                 670         


Ala Leu Arg Ala Ile Lys Met Ile Phe Lys Tyr Leu Pro Arg Ala Tyr 
        675                 680                 685             


Lys Asn Gly Thr Asn Asp Ile Glu Ala Arg Glu Lys Met Ala His Ala 
    690                 695                 700                 


Ser Asn Ile Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Val Cys 
705                 710                 715                 720 


His Ser Met Ala His Lys Leu Gly Ala Met His His Val Pro His Gly 
                725                 730                 735     


Ile Ala Cys Ala Val Leu Ile Glu Glu Val Ile Lys Tyr Asn Ala Thr 
            740                 745                 750         


Asp Cys Pro Thr Lys Gln Thr Ala Phe Pro Gln Tyr Lys Ser Pro Asn 
        755                 760                 765             


Ala Lys Arg Lys Tyr Ala Glu Ile Ala Glu Tyr Leu Asn Leu Lys Gly 
    770                 775                 780                 


Thr Ser Asp Thr Glu Lys Val Thr Ala Leu Ile Glu Ala Ile Ser Lys 
785                 790                 795                 800 


Leu Lys Ile Asp Leu Ser Ile Pro Gln Asn Ile Ser Ala Ala Gly Ile 
                805                 810                 815     


Asn Lys Lys Asp Phe Tyr Asn Thr Leu Asp Lys Met Ser Glu Leu Ala 
            820                 825                 830         


Phe Asp Asp Gln Cys Thr Thr Ala Asn Pro Arg Tyr Pro Leu Ile Ser 
        835                 840                 845             


Glu Leu Lys Asp Ile Tyr Ile Lys Ser Phe 
    850                 855             


<210>  60
<211>  1551
<212>  DNA
<213>  Leptospira interrogans


<220>
<221>  CDS
<222>  (1)..(1551)

<400>  60
atg aca aaa gta gaa act cga ttg gaa att tta gac gta act ttg aga         48
Met Thr Lys Val Glu Thr Arg Leu Glu Ile Leu Asp Val Thr Leu Arg           
1               5                   10                  15                

gac ggg gag cag acc aga ggg gtc agt ttt tcc act tcc gaa aaa cta         96
Asp Gly Glu Gln Thr Arg Gly Val Ser Phe Ser Thr Ser Glu Lys Leu           
            20                  25                  30                    

aat atc gca aaa ttt cta tta caa aaa cta aat gta gat cgg gta gag        144
Asn Ile Ala Lys Phe Leu Leu Gln Lys Leu Asn Val Asp Arg Val Glu           
        35                  40                  45                        

att gcg tct gca aga gtt tct aaa ggg gaa ttg gaa acg gtc caa aaa        192
Ile Ala Ser Ala Arg Val Ser Lys Gly Glu Leu Glu Thr Val Gln Lys           
    50                  55                  60                            

atc atg gaa tgg gct gca aca gaa cag ctt acg gaa aga atc gaa atc        240
Ile Met Glu Trp Ala Ala Thr Glu Gln Leu Thr Glu Arg Ile Glu Ile           
65                  70                  75                  80            

tta ggt ttt gta gac ggg aat aaa acc gta gat tgg atc aaa gat agt        288
Leu Gly Phe Val Asp Gly Asn Lys Thr Val Asp Trp Ile Lys Asp Ser           
                85                  90                  95                

ggg gct aag gtt tta aat ctt ttg act aag gga tcg ctt cat cat tta        336
Gly Ala Lys Val Leu Asn Leu Leu Thr Lys Gly Ser Leu His His Leu           
            100                 105                 110                   

gaa aaa caa tta ggc aaa act ccg aaa gaa ttc ttt aca gac gtt tct        384
Glu Lys Gln Leu Gly Lys Thr Pro Lys Glu Phe Phe Thr Asp Val Ser           
        115                 120                 125                       

ttt gta ata gaa tac gcg atc aaa agc gga ctt aaa ata aac gta tat        432
Phe Val Ile Glu Tyr Ala Ile Lys Ser Gly Leu Lys Ile Asn Val Tyr           
    130                 135                 140                           

tta gaa gat tgg tcc aac ggt ttc aga aac agt cca gat tac gtc aaa        480
Leu Glu Asp Trp Ser Asn Gly Phe Arg Asn Ser Pro Asp Tyr Val Lys           
145                 150                 155                 160           

tcg ctc gta gaa cat cta agt aaa gaa cat ata gaa aga att ttt ctt        528
Ser Leu Val Glu His Leu Ser Lys Glu His Ile Glu Arg Ile Phe Leu           
                165                 170                 175               

cca gac acg tta ggc gtt ctt tcg cca gaa gag acg ttt caa gga gtg        576
Pro Asp Thr Leu Gly Val Leu Ser Pro Glu Glu Thr Phe Gln Gly Val           
            180                 185                 190                   

gat tca ctc att caa aaa tac ccg gat att cat ttt gaa ttt cac gga        624
Asp Ser Leu Ile Gln Lys Tyr Pro Asp Ile His Phe Glu Phe His Gly           
        195                 200                 205                       

cat aac gac tac gat ctt tcc gtg gca aat agt ctt caa gcg att cgt        672
His Asn Asp Tyr Asp Leu Ser Val Ala Asn Ser Leu Gln Ala Ile Arg           
    210                 215                 220                           

gcc gga gtc aaa ggt ctt cac gct tct ata aat ggt ctc gga gaa aga        720
Ala Gly Val Lys Gly Leu His Ala Ser Ile Asn Gly Leu Gly Glu Arg           
225                 230                 235                 240           

gcc gga aat act ccg ttg gaa gca ctc gta acc acg att cat gat aag        768
Ala Gly Asn Thr Pro Leu Glu Ala Leu Val Thr Thr Ile His Asp Lys           
                245                 250                 255               

tct aac tct aaa acg aac ata aac gaa att gca att acg gaa gca agc        816
Ser Asn Ser Lys Thr Asn Ile Asn Glu Ile Ala Ile Thr Glu Ala Ser           
            260                 265                 270                   

cgt ctt gta gaa gta ttc agc gga aaa aga att tct gca aat aga ccg        864
Arg Leu Val Glu Val Phe Ser Gly Lys Arg Ile Ser Ala Asn Arg Pro           
        275                 280                 285                       

atc gta gga gaa gac gtg ttt act cag acc gcg gga gta cac gca gac        912
Ile Val Gly Glu Asp Val Phe Thr Gln Thr Ala Gly Val His Ala Asp           
    290                 295                 300                           

gga gac aaa aaa gga aat tta tac gca aat cct att tta ccg gaa aga        960
Gly Asp Lys Lys Gly Asn Leu Tyr Ala Asn Pro Ile Leu Pro Glu Arg           
305                 310                 315                 320           

ttt ggt agg aaa aga agt tac gcg tta ggc aaa ctt gca ggt aag gcg       1008
Phe Gly Arg Lys Arg Ser Tyr Ala Leu Gly Lys Leu Ala Gly Lys Ala           
                325                 330                 335               

agt atc tcc gaa aat gta aaa caa ctc gga atg gtt tta agt gaa gtg       1056
Ser Ile Ser Glu Asn Val Lys Gln Leu Gly Met Val Leu Ser Glu Val           
            340                 345                 350                   

gtt tta caa aag gtt tta gaa agg gtg atc gaa tta gga gat cag aat       1104
Val Leu Gln Lys Val Leu Glu Arg Val Ile Glu Leu Gly Asp Gln Asn           
        355                 360                 365                       

aaa cta gtg aca cct gaa gat ctt cca ttt atc att gcg gac gtt tct       1152
Lys Leu Val Thr Pro Glu Asp Leu Pro Phe Ile Ile Ala Asp Val Ser           
    370                 375                 380                           

gga aga acc gga gaa aag gta ctt aca atc aaa tct tgt aat att cat       1200
Gly Arg Thr Gly Glu Lys Val Leu Thr Ile Lys Ser Cys Asn Ile His           
385                 390                 395                 400           

tcc gga att gga att cgt cct cac gca caa att gaa ttg gaa tat cag       1248
Ser Gly Ile Gly Ile Arg Pro His Ala Gln Ile Glu Leu Glu Tyr Gln           
                405                 410                 415               

gga aag att cat aag gaa att tct gaa gga gac gga ggg tat gat gcg       1296
Gly Lys Ile His Lys Glu Ile Ser Glu Gly Asp Gly Gly Tyr Asp Ala           
            420                 425                 430                   

ttt atg aat gca ctt act aaa att acg aat cgc ctc ggt att agt att       1344
Phe Met Asn Ala Leu Thr Lys Ile Thr Asn Arg Leu Gly Ile Ser Ile           
        435                 440                 445                       

cct aaa ttg ata gat tac gaa gta agg att cct cct ggt gga aaa aca       1392
Pro Lys Leu Ile Asp Tyr Glu Val Arg Ile Pro Pro Gly Gly Lys Thr           
    450                 455                 460                           

gat gca ctt gta gaa act agg atc acc tgg aac aag tcc tta gat tta       1440
Asp Ala Leu Val Glu Thr Arg Ile Thr Trp Asn Lys Ser Leu Asp Leu           
465                 470                 475                 480           

gaa gag gac cag act ttc aaa acg atg gga gtt cat ccg gat caa acg       1488
Glu Glu Asp Gln Thr Phe Lys Thr Met Gly Val His Pro Asp Gln Thr           
                485                 490                 495               

gtt gca gcg gtt cat gca act gaa aag atg ctc aat caa att cta caa       1536
Val Ala Ala Val His Ala Thr Glu Lys Met Leu Asn Gln Ile Leu Gln           
            500                 505                 510                   

cca tgg caa atc taa                                                   1551
Pro Trp Gln Ile                                                           
        515                                                               


<210>  61
<211>  516
<212>  PRT
<213>  Leptospira interrogans

<400>  61

Met Thr Lys Val Glu Thr Arg Leu Glu Ile Leu Asp Val Thr Leu Arg 
1               5                   10                  15      


Asp Gly Glu Gln Thr Arg Gly Val Ser Phe Ser Thr Ser Glu Lys Leu 
            20                  25                  30          


Asn Ile Ala Lys Phe Leu Leu Gln Lys Leu Asn Val Asp Arg Val Glu 
        35                  40                  45              


Ile Ala Ser Ala Arg Val Ser Lys Gly Glu Leu Glu Thr Val Gln Lys 
    50                  55                  60                  


Ile Met Glu Trp Ala Ala Thr Glu Gln Leu Thr Glu Arg Ile Glu Ile 
65                  70                  75                  80  


Leu Gly Phe Val Asp Gly Asn Lys Thr Val Asp Trp Ile Lys Asp Ser 
                85                  90                  95      


Gly Ala Lys Val Leu Asn Leu Leu Thr Lys Gly Ser Leu His His Leu 
            100                 105                 110         


Glu Lys Gln Leu Gly Lys Thr Pro Lys Glu Phe Phe Thr Asp Val Ser 
        115                 120                 125             


Phe Val Ile Glu Tyr Ala Ile Lys Ser Gly Leu Lys Ile Asn Val Tyr 
    130                 135                 140                 


Leu Glu Asp Trp Ser Asn Gly Phe Arg Asn Ser Pro Asp Tyr Val Lys 
145                 150                 155                 160 


Ser Leu Val Glu His Leu Ser Lys Glu His Ile Glu Arg Ile Phe Leu 
                165                 170                 175     


Pro Asp Thr Leu Gly Val Leu Ser Pro Glu Glu Thr Phe Gln Gly Val 
            180                 185                 190         


Asp Ser Leu Ile Gln Lys Tyr Pro Asp Ile His Phe Glu Phe His Gly 
        195                 200                 205             


His Asn Asp Tyr Asp Leu Ser Val Ala Asn Ser Leu Gln Ala Ile Arg 
    210                 215                 220                 


Ala Gly Val Lys Gly Leu His Ala Ser Ile Asn Gly Leu Gly Glu Arg 
225                 230                 235                 240 


Ala Gly Asn Thr Pro Leu Glu Ala Leu Val Thr Thr Ile His Asp Lys 
                245                 250                 255     


Ser Asn Ser Lys Thr Asn Ile Asn Glu Ile Ala Ile Thr Glu Ala Ser 
            260                 265                 270         


Arg Leu Val Glu Val Phe Ser Gly Lys Arg Ile Ser Ala Asn Arg Pro 
        275                 280                 285             


Ile Val Gly Glu Asp Val Phe Thr Gln Thr Ala Gly Val His Ala Asp 
    290                 295                 300                 


Gly Asp Lys Lys Gly Asn Leu Tyr Ala Asn Pro Ile Leu Pro Glu Arg 
305                 310                 315                 320 


Phe Gly Arg Lys Arg Ser Tyr Ala Leu Gly Lys Leu Ala Gly Lys Ala 
                325                 330                 335     


Ser Ile Ser Glu Asn Val Lys Gln Leu Gly Met Val Leu Ser Glu Val 
            340                 345                 350         


Val Leu Gln Lys Val Leu Glu Arg Val Ile Glu Leu Gly Asp Gln Asn 
        355                 360                 365             


Lys Leu Val Thr Pro Glu Asp Leu Pro Phe Ile Ile Ala Asp Val Ser 
    370                 375                 380                 


Gly Arg Thr Gly Glu Lys Val Leu Thr Ile Lys Ser Cys Asn Ile His 
385                 390                 395                 400 


Ser Gly Ile Gly Ile Arg Pro His Ala Gln Ile Glu Leu Glu Tyr Gln 
                405                 410                 415     


Gly Lys Ile His Lys Glu Ile Ser Glu Gly Asp Gly Gly Tyr Asp Ala 
            420                 425                 430         


Phe Met Asn Ala Leu Thr Lys Ile Thr Asn Arg Leu Gly Ile Ser Ile 
        435                 440                 445             


Pro Lys Leu Ile Asp Tyr Glu Val Arg Ile Pro Pro Gly Gly Lys Thr 
    450                 455                 460                 


Asp Ala Leu Val Glu Thr Arg Ile Thr Trp Asn Lys Ser Leu Asp Leu 
465                 470                 475                 480 


Glu Glu Asp Gln Thr Phe Lys Thr Met Gly Val His Pro Asp Gln Thr 
                485                 490                 495     


Val Ala Ala Val His Ala Thr Glu Lys Met Leu Asn Gln Ile Leu Gln 
            500                 505                 510         


Pro Trp Gln Ile 
        515     


<210>  62
<211>  1398
<212>  DNA
<213>  Leptospira interrogans


<220>
<221>  CDS
<222>  (1)..(1398)

<400>  62
atg aag aca atg ttc gaa aaa att tgg gaa gat cat cta gtc gga gaa         48
Met Lys Thr Met Phe Glu Lys Ile Trp Glu Asp His Leu Val Gly Glu           
1               5                   10                  15                

cta gat gct gga tcc tat cta atc tat ata gat cgc cat ctc att cat         96
Leu Asp Ala Gly Ser Tyr Leu Ile Tyr Ile Asp Arg His Leu Ile His           
            20                  25                  30                    

gaa gtt aca agt cct cag gcg ttt gaa gga ctt aaa ctt gca ggc aga        144
Glu Val Thr Ser Pro Gln Ala Phe Glu Gly Leu Lys Leu Ala Gly Arg           
        35                  40                  45                        

aag gtt cgt cgt cct gaa gct act ttt gcc aca atg gat cat aac gtt        192
Lys Val Arg Arg Pro Glu Ala Thr Phe Ala Thr Met Asp His Asn Val           
    50                  55                  60                            

tct act aga aca cgt gat tta agt ctg gcc gat cct gtt tcc gca att        240
Ser Thr Arg Thr Arg Asp Leu Ser Leu Ala Asp Pro Val Ser Ala Ile           
65                  70                  75                  80            

caa atg cag act tta aaa aag aac tgc gac gaa aac gga atc cgc gtt        288
Gln Met Gln Thr Leu Lys Lys Asn Cys Asp Glu Asn Gly Ile Arg Val           
                85                  90                  95                

tat gat ttt caa aac cct gac caa gga atc att cac gta atc gct cct        336
Tyr Asp Phe Gln Asn Pro Asp Gln Gly Ile Ile His Val Ile Ala Pro           
            100                 105                 110                   

gaa atg gga ctg act cat cct gga atg aca atc gta tgc gga gat tct        384
Glu Met Gly Leu Thr His Pro Gly Met Thr Ile Val Cys Gly Asp Ser           
        115                 120                 125                       

cat act tct aca cac ggt gcg ttt ggt gcg ctt gct ttc ggg atc gga        432
His Thr Ser Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile Gly           
    130                 135                 140                           

acc agc gaa gta gag cac gtt ctt gcg act caa acc tta gtt caa aaa        480
Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Val Gln Lys           
145                 150                 155                 160           

aga gca aaa aca atg gag att aga gtc gat gga aaa ctt tcc gat aag        528
Arg Ala Lys Thr Met Glu Ile Arg Val Asp Gly Lys Leu Ser Asp Lys           
                165                 170                 175               

gtc aca gca aaa gac atc att ctt gcg atc att gga aaa att gga acc        576
Val Thr Ala Lys Asp Ile Ile Leu Ala Ile Ile Gly Lys Ile Gly Thr           
            180                 185                 190                   

gca ggt gcg aca ggt tat gtg atc gaa tat aga ggt tct gca att caa        624
Ala Gly Ala Thr Gly Tyr Val Ile Glu Tyr Arg Gly Ser Ala Ile Gln           
        195                 200                 205                       

gcc ctc agt atg gaa gct aga atg act att tgt aat atg tct atc gaa        672
Ala Leu Ser Met Glu Ala Arg Met Thr Ile Cys Asn Met Ser Ile Glu           
    210                 215                 220                           

gcg gga gct aga gca ggt tta atc gca cca gat gaa act act ttt aat        720
Ala Gly Ala Arg Ala Gly Leu Ile Ala Pro Asp Glu Thr Thr Phe Asn           
225                 230                 235                 240           

tat att caa gga aag gac ttt tct cca aaa gga gtc gaa tgg gat ctt        768
Tyr Ile Gln Gly Lys Asp Phe Ser Pro Lys Gly Val Glu Trp Asp Leu           
                245                 250                 255               

gcg gtc aaa aaa tgg aaa cac tat gta acg gac gaa ggt gct aaa ttt        816
Ala Val Lys Lys Trp Lys His Tyr Val Thr Asp Glu Gly Ala Lys Phe           
            260                 265                 270                   

gat aga acc gta att ctt cat gca gat gaa atc gct cct atg gta act        864
Asp Arg Thr Val Ile Leu His Ala Asp Glu Ile Ala Pro Met Val Thr           
        275                 280                 285                       

tgg gga act tct ccc agt cag gtt gtt tcg ata aaa gga gtc gtt cca        912
Trp Gly Thr Ser Pro Ser Gln Val Val Ser Ile Lys Gly Val Val Pro           
    290                 295                 300                           

gat cca aaa gat gca aat gat ccg gtg gaa aaa att gga att gag tct        960
Asp Pro Lys Asp Ala Asn Asp Pro Val Glu Lys Ile Gly Ile Glu Ser           
305                 310                 315                 320           

gcg ctt aaa tat atg gat ctc aaa tcg ggc cag aag ata gaa gac att       1008
Ala Leu Lys Tyr Met Asp Leu Lys Ser Gly Gln Lys Ile Glu Asp Ile           
                325                 330                 335               

tca att aat aaa gtg ttt atc ggt tcc tgt act aat tct aga atc gaa       1056
Ser Ile Asn Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile Glu           
            340                 345                 350                   

gat tta aga gcg gcc gct gct acc gta aaa gga aaa aaa gtt tcc tct       1104
Asp Leu Arg Ala Ala Ala Ala Thr Val Lys Gly Lys Lys Val Ser Ser           
        355                 360                 365                       

aag gtt cag gcg att gtg gtt ccc ggt tca ggc aga gtc aaa cgt cag       1152
Lys Val Gln Ala Ile Val Val Pro Gly Ser Gly Arg Val Lys Arg Gln           
    370                 375                 380                           

gcg gaa caa gaa ggt ctg gat aaa att ttt acc gcg gcc ggt ttt gaa       1200
Ala Glu Gln Glu Gly Leu Asp Lys Ile Phe Thr Ala Ala Gly Phe Glu           
385                 390                 395                 400           

tgg aga aat cca ggc tgt tct atg tgt ctt gcg atg aac gac gac gta       1248
Trp Arg Asn Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asp Asp Val           
                405                 410                 415               

tta gaa ccg gga gat cgt tgt gct tct act tct aac cga aac ttt gaa       1296
Leu Glu Pro Gly Asp Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe Glu           
            420                 425                 430                   

ggt cgt caa gga aaa ggt gga aga acc cat cta gta gga ccg gaa atg       1344
Gly Arg Gln Gly Lys Gly Gly Arg Thr His Leu Val Gly Pro Glu Met           
        435                 440                 445                       

gcc gcc gcc gcg gct atc gaa ggc cat ttt gtg gat att cga aac tgg       1392
Ala Ala Ala Ala Ala Ile Glu Gly His Phe Val Asp Ile Arg Asn Trp           
    450                 455                 460                           

aaa taa                                                               1398
Lys                                                                       
465                                                                       


<210>  63
<211>  465
<212>  PRT
<213>  Leptospira interrogans

<400>  63

Met Lys Thr Met Phe Glu Lys Ile Trp Glu Asp His Leu Val Gly Glu 
1               5                   10                  15      


Leu Asp Ala Gly Ser Tyr Leu Ile Tyr Ile Asp Arg His Leu Ile His 
            20                  25                  30          


Glu Val Thr Ser Pro Gln Ala Phe Glu Gly Leu Lys Leu Ala Gly Arg 
        35                  40                  45              


Lys Val Arg Arg Pro Glu Ala Thr Phe Ala Thr Met Asp His Asn Val 
    50                  55                  60                  


Ser Thr Arg Thr Arg Asp Leu Ser Leu Ala Asp Pro Val Ser Ala Ile 
65                  70                  75                  80  


Gln Met Gln Thr Leu Lys Lys Asn Cys Asp Glu Asn Gly Ile Arg Val 
                85                  90                  95      


Tyr Asp Phe Gln Asn Pro Asp Gln Gly Ile Ile His Val Ile Ala Pro 
            100                 105                 110         


Glu Met Gly Leu Thr His Pro Gly Met Thr Ile Val Cys Gly Asp Ser 
        115                 120                 125             


His Thr Ser Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile Gly 
    130                 135                 140                 


Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Val Gln Lys 
145                 150                 155                 160 


Arg Ala Lys Thr Met Glu Ile Arg Val Asp Gly Lys Leu Ser Asp Lys 
                165                 170                 175     


Val Thr Ala Lys Asp Ile Ile Leu Ala Ile Ile Gly Lys Ile Gly Thr 
            180                 185                 190         


Ala Gly Ala Thr Gly Tyr Val Ile Glu Tyr Arg Gly Ser Ala Ile Gln 
        195                 200                 205             


Ala Leu Ser Met Glu Ala Arg Met Thr Ile Cys Asn Met Ser Ile Glu 
    210                 215                 220                 


Ala Gly Ala Arg Ala Gly Leu Ile Ala Pro Asp Glu Thr Thr Phe Asn 
225                 230                 235                 240 


Tyr Ile Gln Gly Lys Asp Phe Ser Pro Lys Gly Val Glu Trp Asp Leu 
                245                 250                 255     


Ala Val Lys Lys Trp Lys His Tyr Val Thr Asp Glu Gly Ala Lys Phe 
            260                 265                 270         


Asp Arg Thr Val Ile Leu His Ala Asp Glu Ile Ala Pro Met Val Thr 
        275                 280                 285             


Trp Gly Thr Ser Pro Ser Gln Val Val Ser Ile Lys Gly Val Val Pro 
    290                 295                 300                 


Asp Pro Lys Asp Ala Asn Asp Pro Val Glu Lys Ile Gly Ile Glu Ser 
305                 310                 315                 320 


Ala Leu Lys Tyr Met Asp Leu Lys Ser Gly Gln Lys Ile Glu Asp Ile 
                325                 330                 335     


Ser Ile Asn Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile Glu 
            340                 345                 350         


Asp Leu Arg Ala Ala Ala Ala Thr Val Lys Gly Lys Lys Val Ser Ser 
        355                 360                 365             


Lys Val Gln Ala Ile Val Val Pro Gly Ser Gly Arg Val Lys Arg Gln 
    370                 375                 380                 


Ala Glu Gln Glu Gly Leu Asp Lys Ile Phe Thr Ala Ala Gly Phe Glu 
385                 390                 395                 400 


Trp Arg Asn Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asp Asp Val 
                405                 410                 415     


Leu Glu Pro Gly Asp Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe Glu 
            420                 425                 430         


Gly Arg Gln Gly Lys Gly Gly Arg Thr His Leu Val Gly Pro Glu Met 
        435                 440                 445             


Ala Ala Ala Ala Ala Ile Glu Gly His Phe Val Asp Ile Arg Asn Trp 
    450                 455                 460                 


Lys 
465 


<210>  64
<211>  621
<212>  DNA
<213>  Leptospira interrogans


<220>
<221>  CDS
<222>  (1)..(621)

<400>  64
atg aaa ccc ttt act ata tta aat gga att gcc gcc tta ctg gac aga         48
Met Lys Pro Phe Thr Ile Leu Asn Gly Ile Ala Ala Leu Leu Asp Arg           
1               5                   10                  15                

ccc aac gtg gat acg gat cag atc att cca aaa caa ttt tta cgg aag         96
Pro Asn Val Asp Thr Asp Gln Ile Ile Pro Lys Gln Phe Leu Arg Lys           
            20                  25                  30                    

ata gaa cga acc ggt ttc gga gtt cat ctg ttt cac gat tgg aga tac        144
Ile Glu Arg Thr Gly Phe Gly Val His Leu Phe His Asp Trp Arg Tyr           
        35                  40                  45                        

tta gac gac gcg ggt acc aaa ctc aat cct gat ttt tcc ctc aat caa        192
Leu Asp Asp Ala Gly Thr Lys Leu Asn Pro Asp Phe Ser Leu Asn Gln           
    50                  55                  60                            

gaa cga tat aag gga gct tct atc ctt atc acc aga gat aac ttt ggt        240
Glu Arg Tyr Lys Gly Ala Ser Ile Leu Ile Thr Arg Asp Asn Phe Gly           
65                  70                  75                  80            

tgt gga tct tcc aga gaa cac gct cct tgg gct tta gaa gac tac ggg        288
Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Glu Asp Tyr Gly           
                85                  90                  95                

ttt agg gca atc att gct cct tct tac gcg gat att ttt ttc aac aac        336
Phe Arg Ala Ile Ile Ala Pro Ser Tyr Ala Asp Ile Phe Phe Asn Asn           
            100                 105                 110                   

tgc ttt aaa aac gga atg ctt cca gtc att tta aaa tcg gaa gaa gta        384
Cys Phe Lys Asn Gly Met Leu Pro Val Ile Leu Lys Ser Glu Glu Val           
        115                 120                 125                       

gaa gag ctg ttc cat ttg gtt tcg act aac gta gga gcg aaa gtc ata        432
Glu Glu Leu Phe His Leu Val Ser Thr Asn Val Gly Ala Lys Val Ile           
    130                 135                 140                           

gtg gat ctg gac aaa caa act gta acc gga ccg act gga aaa ata tat        480
Val Asp Leu Asp Lys Gln Thr Val Thr Gly Pro Thr Gly Lys Ile Tyr           
145                 150                 155                 160           

tat ttt gaa gtg gat tct ttt cgt aaa tac tgt ctt tat aac gga ctt        528
Tyr Phe Glu Val Asp Ser Phe Arg Lys Tyr Cys Leu Tyr Asn Gly Leu           
                165                 170                 175               

gat gac ata ggt cta act cta aaa caa gaa agt aaa att gga gag ttt        576
Asp Asp Ile Gly Leu Thr Leu Lys Gln Glu Ser Lys Ile Gly Glu Phe           
            180                 185                 190                   

gaa aaa aag cag aaa gaa gtt gaa cct tgg tta tac gcc ata taa            621
Glu Lys Lys Gln Lys Glu Val Glu Pro Trp Leu Tyr Ala Ile                   
        195                 200                 205                       


<210>  65
<211>  206
<212>  PRT
<213>  Leptospira interrogans

<400>  65

Met Lys Pro Phe Thr Ile Leu Asn Gly Ile Ala Ala Leu Leu Asp Arg 
1               5                   10                  15      


Pro Asn Val Asp Thr Asp Gln Ile Ile Pro Lys Gln Phe Leu Arg Lys 
            20                  25                  30          


Ile Glu Arg Thr Gly Phe Gly Val His Leu Phe His Asp Trp Arg Tyr 
        35                  40                  45              


Leu Asp Asp Ala Gly Thr Lys Leu Asn Pro Asp Phe Ser Leu Asn Gln 
    50                  55                  60                  


Glu Arg Tyr Lys Gly Ala Ser Ile Leu Ile Thr Arg Asp Asn Phe Gly 
65                  70                  75                  80  


Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Glu Asp Tyr Gly 
                85                  90                  95      


Phe Arg Ala Ile Ile Ala Pro Ser Tyr Ala Asp Ile Phe Phe Asn Asn 
            100                 105                 110         


Cys Phe Lys Asn Gly Met Leu Pro Val Ile Leu Lys Ser Glu Glu Val 
        115                 120                 125             


Glu Glu Leu Phe His Leu Val Ser Thr Asn Val Gly Ala Lys Val Ile 
    130                 135                 140                 


Val Asp Leu Asp Lys Gln Thr Val Thr Gly Pro Thr Gly Lys Ile Tyr 
145                 150                 155                 160 


Tyr Phe Glu Val Asp Ser Phe Arg Lys Tyr Cys Leu Tyr Asn Gly Leu 
                165                 170                 175     


Asp Asp Ile Gly Leu Thr Leu Lys Gln Glu Ser Lys Ile Gly Glu Phe 
            180                 185                 190         


Glu Lys Lys Gln Lys Glu Val Glu Pro Trp Leu Tyr Ala Ile 
        195                 200                 205     


<210>  66
<211>  1077
<212>  DNA
<213>  Leptospira interrogans


<220>
<221>  CDS
<222>  (1)..(1077)

<400>  66
atg aag aat gta gca gta ctt tca gga gac gga atc gga ccg gaa gtc         48
Met Lys Asn Val Ala Val Leu Ser Gly Asp Gly Ile Gly Pro Glu Val           
1               5                   10                  15                

atg gag ata gcc atc tcc gtt ttg aaa aag gct ctc ggt gca aaa gtt         96
Met Glu Ile Ala Ile Ser Val Leu Lys Lys Ala Leu Gly Ala Lys Val           
            20                  25                  30                    

tcc gag ttt caa ttt aaa gaa gga ttt gta ggt gga atc gca atc gat        144
Ser Glu Phe Gln Phe Lys Glu Gly Phe Val Gly Gly Ile Ala Ile Asp           
        35                  40                  45                        

aaa act gga cac cca ctt cca ccg gaa act ctt aaa cta tgt gaa gaa        192
Lys Thr Gly His Pro Leu Pro Pro Glu Thr Leu Lys Leu Cys Glu Glu           
    50                  55                  60                            

tct tcc gca att ctt ttc gga agt gtg gga ggt cct aaa tgg gaa aca        240
Ser Ser Ala Ile Leu Phe Gly Ser Val Gly Gly Pro Lys Trp Glu Thr           
65                  70                  75                  80            

ctc cct ccg gaa aaa caa ccg gaa cga ggg gca ctt cta cct ttg aga        288
Leu Pro Pro Glu Lys Gln Pro Glu Arg Gly Ala Leu Leu Pro Leu Arg           
                85                  90                  95                

aaa cat ttt gat cta ttt gca aac tta aga cct gcg atc att tat cca        336
Lys His Phe Asp Leu Phe Ala Asn Leu Arg Pro Ala Ile Ile Tyr Pro           
            100                 105                 110                   

gag ttg aaa aat gct tct cca gtt cgt tct gat att att gga aac gga        384
Glu Leu Lys Asn Ala Ser Pro Val Arg Ser Asp Ile Ile Gly Asn Gly           
        115                 120                 125                       

tta gat att ctc ata tta aga gag tta acc gga gga att tat ttt gga        432
Leu Asp Ile Leu Ile Leu Arg Glu Leu Thr Gly Gly Ile Tyr Phe Gly           
    130                 135                 140                           

caa cca aaa gga aga gaa gga tca ggt cag gaa gaa ttt gca tac gac        480
Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Glu Glu Phe Ala Tyr Asp           
145                 150                 155                 160           

acg atg aag tat tcc aga aga gaa atc gaa agg att gct aaa gtc gca        528
Thr Met Lys Tyr Ser Arg Arg Glu Ile Glu Arg Ile Ala Lys Val Ala           
                165                 170                 175               

ttc cag gcg gcc aga aaa aga aat aat aaa gtg act agt atc gat aaa        576
Phe Gln Ala Ala Arg Lys Arg Asn Asn Lys Val Thr Ser Ile Asp Lys           
            180                 185                 190                   

gca aac gtc ttg act act tcc gtt ttt tgg aag gaa gta gta atc gaa        624
Ala Asn Val Leu Thr Thr Ser Val Phe Trp Lys Glu Val Val Ile Glu           
        195                 200                 205                       

ttg cat aag aaa gaa ttt tca gac gtc caa ttg aat cat ctt tat gtg        672
Leu His Lys Lys Glu Phe Ser Asp Val Gln Leu Asn His Leu Tyr Val           
    210                 215                 220                           

gac aat gcg gcg atg cag tta atc gta aat ccg aaa caa ttc gac gtg        720
Asp Asn Ala Ala Met Gln Leu Ile Val Asn Pro Lys Gln Phe Asp Val           
225                 230                 235                 240           

gtt ctt tgt gag aat atg ttt ggt gat att ctt tcg gac gag gct tcc        768
Val Leu Cys Glu Asn Met Phe Gly Asp Ile Leu Ser Asp Glu Ala Ser           
                245                 250                 255               

atc att acg ggt tca atc gga atg ctt cct tct gcc tct ctt tcc gaa        816
Ile Ile Thr Gly Ser Ile Gly Met Leu Pro Ser Ala Ser Leu Ser Glu           
            260                 265                 270                   

tct gga ttt gga ttg tat gaa cct tct ggt ggt tct gcg ccg gac ata        864
Ser Gly Phe Gly Leu Tyr Glu Pro Ser Gly Gly Ser Ala Pro Asp Ile           
        275                 280                 285                       

gcc gga aaa gga gtg gca aat ccg att gct caa gta ttg agt gcg gcg        912
Ala Gly Lys Gly Val Ala Asn Pro Ile Ala Gln Val Leu Ser Ala Ala           
    290                 295                 300                           

ttg atg tta cgt tat tct ttt tct atg gaa gaa gaa gca aac aag ata        960
Leu Met Leu Arg Tyr Ser Phe Ser Met Glu Glu Glu Ala Asn Lys Ile           
305                 310                 315                 320           

gaa acc gcc gtg cgt aaa acg att gcc tcc gga aaa aga acc aga gac       1008
Glu Thr Ala Val Arg Lys Thr Ile Ala Ser Gly Lys Arg Thr Arg Asp           
                325                 330                 335               

ata gcg gaa gta gga tct acg atc gta gga act aaa gaa atc ggt caa       1056
Ile Ala Glu Val Gly Ser Thr Ile Val Gly Thr Lys Glu Ile Gly Gln           
            340                 345                 350                   

ttg atc gaa tcc ttt ctc taa                                           1077
Leu Ile Glu Ser Phe Leu                                                   
        355                                                               


<210>  67
<211>  358
<212>  PRT
<213>  Leptospira interrogans

<400>  67

Met Lys Asn Val Ala Val Leu Ser Gly Asp Gly Ile Gly Pro Glu Val 
1               5                   10                  15      


Met Glu Ile Ala Ile Ser Val Leu Lys Lys Ala Leu Gly Ala Lys Val 
            20                  25                  30          


Ser Glu Phe Gln Phe Lys Glu Gly Phe Val Gly Gly Ile Ala Ile Asp 
        35                  40                  45              


Lys Thr Gly His Pro Leu Pro Pro Glu Thr Leu Lys Leu Cys Glu Glu 
    50                  55                  60                  


Ser Ser Ala Ile Leu Phe Gly Ser Val Gly Gly Pro Lys Trp Glu Thr 
65                  70                  75                  80  


Leu Pro Pro Glu Lys Gln Pro Glu Arg Gly Ala Leu Leu Pro Leu Arg 
                85                  90                  95      


Lys His Phe Asp Leu Phe Ala Asn Leu Arg Pro Ala Ile Ile Tyr Pro 
            100                 105                 110         


Glu Leu Lys Asn Ala Ser Pro Val Arg Ser Asp Ile Ile Gly Asn Gly 
        115                 120                 125             


Leu Asp Ile Leu Ile Leu Arg Glu Leu Thr Gly Gly Ile Tyr Phe Gly 
    130                 135                 140                 


Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Glu Glu Phe Ala Tyr Asp 
145                 150                 155                 160 


Thr Met Lys Tyr Ser Arg Arg Glu Ile Glu Arg Ile Ala Lys Val Ala 
                165                 170                 175     


Phe Gln Ala Ala Arg Lys Arg Asn Asn Lys Val Thr Ser Ile Asp Lys 
            180                 185                 190         


Ala Asn Val Leu Thr Thr Ser Val Phe Trp Lys Glu Val Val Ile Glu 
        195                 200                 205             


Leu His Lys Lys Glu Phe Ser Asp Val Gln Leu Asn His Leu Tyr Val 
    210                 215                 220                 


Asp Asn Ala Ala Met Gln Leu Ile Val Asn Pro Lys Gln Phe Asp Val 
225                 230                 235                 240 


Val Leu Cys Glu Asn Met Phe Gly Asp Ile Leu Ser Asp Glu Ala Ser 
                245                 250                 255     


Ile Ile Thr Gly Ser Ile Gly Met Leu Pro Ser Ala Ser Leu Ser Glu 
            260                 265                 270         


Ser Gly Phe Gly Leu Tyr Glu Pro Ser Gly Gly Ser Ala Pro Asp Ile 
        275                 280                 285             


Ala Gly Lys Gly Val Ala Asn Pro Ile Ala Gln Val Leu Ser Ala Ala 
    290                 295                 300                 


Leu Met Leu Arg Tyr Ser Phe Ser Met Glu Glu Glu Ala Asn Lys Ile 
305                 310                 315                 320 


Glu Thr Ala Val Arg Lys Thr Ile Ala Ser Gly Lys Arg Thr Arg Asp 
                325                 330                 335     


Ile Ala Glu Val Gly Ser Thr Ile Val Gly Thr Lys Glu Ile Gly Gln 
            340                 345                 350         


Leu Ile Glu Ser Phe Leu 
        355             


<210>  68
<211>  1161
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1161)

<400>  68
atg aca tcg gaa aac ccg tta ctg gcg ctg cga gag aaa atc agc gcg         48
Met Thr Ser Glu Asn Pro Leu Leu Ala Leu Arg Glu Lys Ile Ser Ala           
1               5                   10                  15                

ctg gat gaa aaa tta tta gcg tta ctg gca gaa cgg cgc gaa ctg gcc         96
Leu Asp Glu Lys Leu Leu Ala Leu Leu Ala Glu Arg Arg Glu Leu Ala           
            20                  25                  30                    

gtc gag gtg gga aaa gcc aaa ctg ctc tcg cat cgc ccg gta cgt gat        144
Val Glu Val Gly Lys Ala Lys Leu Leu Ser His Arg Pro Val Arg Asp           
        35                  40                  45                        

att gat cgt gaa cgc gat ttg ctg gaa aga tta att acg ctc ggt aaa        192
Ile Asp Arg Glu Arg Asp Leu Leu Glu Arg Leu Ile Thr Leu Gly Lys           
    50                  55                  60                            

gcg cac cat ctg gac gcc cat tac att act cgc ctg ttc cag ctc atc        240
Ala His His Leu Asp Ala His Tyr Ile Thr Arg Leu Phe Gln Leu Ile           
65                  70                  75                  80            

att gaa gat tcc gta tta act cag cag gct ttg ctc caa caa cat ctc        288
Ile Glu Asp Ser Val Leu Thr Gln Gln Ala Leu Leu Gln Gln His Leu           
                85                  90                  95                

aat aaa att aat ccg cac tca gca cgc atc gct ttt ctc ggc ccc aaa        336
Asn Lys Ile Asn Pro His Ser Ala Arg Ile Ala Phe Leu Gly Pro Lys           
            100                 105                 110                   

ggt tct tat tcc cat ctt gcg gcg cgc cag tat gct gcc cgt cac ttt        384
Gly Ser Tyr Ser His Leu Ala Ala Arg Gln Tyr Ala Ala Arg His Phe           
        115                 120                 125                       

gag caa ttc att gaa agt ggc tgc gcc aaa ttt gcc gat att ttt aat        432
Glu Gln Phe Ile Glu Ser Gly Cys Ala Lys Phe Ala Asp Ile Phe Asn           
    130                 135                 140                           

cag gtg gaa acc ggc cag gcc gac tat gcc gtc gta ccg att gaa aat        480
Gln Val Glu Thr Gly Gln Ala Asp Tyr Ala Val Val Pro Ile Glu Asn           
145                 150                 155                 160           

acc agc tcc ggt gcc ata aac gac gtt tac gat ctg ctg caa cat acc        528
Thr Ser Ser Gly Ala Ile Asn Asp Val Tyr Asp Leu Leu Gln His Thr           
                165                 170                 175               

agc ttg tcg att gtt ggc gag atg acg tta act atc gac cat tgt ttg        576
Ser Leu Ser Ile Val Gly Glu Met Thr Leu Thr Ile Asp His Cys Leu           
            180                 185                 190                   

ttg gtc tcc ggc act act gat tta tcc acc atc aat acg gtc tac agc        624
Leu Val Ser Gly Thr Thr Asp Leu Ser Thr Ile Asn Thr Val Tyr Ser           
        195                 200                 205                       

cat ccg cag cca ttc cag caa tgc agc aaa ttc ctt aat cgt tat ccg        672
His Pro Gln Pro Phe Gln Gln Cys Ser Lys Phe Leu Asn Arg Tyr Pro           
    210                 215                 220                           

cac tgg aag att gaa tat acc gaa agt acg tct gcg gca atg gaa aag        720
His Trp Lys Ile Glu Tyr Thr Glu Ser Thr Ser Ala Ala Met Glu Lys           
225                 230                 235                 240           

gtt gca cag gca aaa tca ccg cat gtt gct gcg ttg gga agc gaa gct        768
Val Ala Gln Ala Lys Ser Pro His Val Ala Ala Leu Gly Ser Glu Ala           
                245                 250                 255               

ggc ggc act ttg tac ggt ttg cag gta ctg gag cgt att gaa gca aat        816
Gly Gly Thr Leu Tyr Gly Leu Gln Val Leu Glu Arg Ile Glu Ala Asn           
            260                 265                 270                   

cag cga caa aac ttc acc cga ttt gtg gtg ttg gcg cgt aaa gcc att        864
Gln Arg Gln Asn Phe Thr Arg Phe Val Val Leu Ala Arg Lys Ala Ile           
        275                 280                 285                       

aac gtg tct gat cag gtt ccg gcg aaa acc acg ttg tta atg gcg acc        912
Asn Val Ser Asp Gln Val Pro Ala Lys Thr Thr Leu Leu Met Ala Thr           
    290                 295                 300                           

ggg caa caa gcc ggt gcg ctg gtt gaa gcg ttg ctg gta ctg cgc aac        960
Gly Gln Gln Ala Gly Ala Leu Val Glu Ala Leu Leu Val Leu Arg Asn           
305                 310                 315                 320           

cac aat ctg att atg acc cgt ctg gaa tca cgc ccg att cac ggt aat       1008
His Asn Leu Ile Met Thr Arg Leu Glu Ser Arg Pro Ile His Gly Asn           
                325                 330                 335               

cca tgg gaa gag atg ttc tat ctg gat att cag gcc aat ctt gaa tca       1056
Pro Trp Glu Glu Met Phe Tyr Leu Asp Ile Gln Ala Asn Leu Glu Ser           
            340                 345                 350                   

gcg gaa atg caa aaa gca ttg aaa gag tta ggg gaa atc acc cgt tca       1104
Ala Glu Met Gln Lys Ala Leu Lys Glu Leu Gly Glu Ile Thr Arg Ser           
        355                 360                 365                       

atg aag gta ttg ggc tgt tac cca agt gag aac gta gtg cct gtt gat       1152
Met Lys Val Leu Gly Cys Tyr Pro Ser Glu Asn Val Val Pro Val Asp           
    370                 375                 380                           

cca acc tga                                                           1161
Pro Thr                                                                   
385                                                                       


<210>  69
<211>  386
<212>  PRT
<213>  Escherichia coli

<400>  69

Met Thr Ser Glu Asn Pro Leu Leu Ala Leu Arg Glu Lys Ile Ser Ala 
1               5                   10                  15      


Leu Asp Glu Lys Leu Leu Ala Leu Leu Ala Glu Arg Arg Glu Leu Ala 
            20                  25                  30          


Val Glu Val Gly Lys Ala Lys Leu Leu Ser His Arg Pro Val Arg Asp 
        35                  40                  45              


Ile Asp Arg Glu Arg Asp Leu Leu Glu Arg Leu Ile Thr Leu Gly Lys 
    50                  55                  60                  


Ala His His Leu Asp Ala His Tyr Ile Thr Arg Leu Phe Gln Leu Ile 
65                  70                  75                  80  


Ile Glu Asp Ser Val Leu Thr Gln Gln Ala Leu Leu Gln Gln His Leu 
                85                  90                  95      


Asn Lys Ile Asn Pro His Ser Ala Arg Ile Ala Phe Leu Gly Pro Lys 
            100                 105                 110         


Gly Ser Tyr Ser His Leu Ala Ala Arg Gln Tyr Ala Ala Arg His Phe 
        115                 120                 125             


Glu Gln Phe Ile Glu Ser Gly Cys Ala Lys Phe Ala Asp Ile Phe Asn 
    130                 135                 140                 


Gln Val Glu Thr Gly Gln Ala Asp Tyr Ala Val Val Pro Ile Glu Asn 
145                 150                 155                 160 


Thr Ser Ser Gly Ala Ile Asn Asp Val Tyr Asp Leu Leu Gln His Thr 
                165                 170                 175     


Ser Leu Ser Ile Val Gly Glu Met Thr Leu Thr Ile Asp His Cys Leu 
            180                 185                 190         


Leu Val Ser Gly Thr Thr Asp Leu Ser Thr Ile Asn Thr Val Tyr Ser 
        195                 200                 205             


His Pro Gln Pro Phe Gln Gln Cys Ser Lys Phe Leu Asn Arg Tyr Pro 
    210                 215                 220                 


His Trp Lys Ile Glu Tyr Thr Glu Ser Thr Ser Ala Ala Met Glu Lys 
225                 230                 235                 240 


Val Ala Gln Ala Lys Ser Pro His Val Ala Ala Leu Gly Ser Glu Ala 
                245                 250                 255     


Gly Gly Thr Leu Tyr Gly Leu Gln Val Leu Glu Arg Ile Glu Ala Asn 
            260                 265                 270         


Gln Arg Gln Asn Phe Thr Arg Phe Val Val Leu Ala Arg Lys Ala Ile 
        275                 280                 285             


Asn Val Ser Asp Gln Val Pro Ala Lys Thr Thr Leu Leu Met Ala Thr 
    290                 295                 300                 


Gly Gln Gln Ala Gly Ala Leu Val Glu Ala Leu Leu Val Leu Arg Asn 
305                 310                 315                 320 


His Asn Leu Ile Met Thr Arg Leu Glu Ser Arg Pro Ile His Gly Asn 
                325                 330                 335     


Pro Trp Glu Glu Met Phe Tyr Leu Asp Ile Gln Ala Asn Leu Glu Ser 
            340                 345                 350         


Ala Glu Met Gln Lys Ala Leu Lys Glu Leu Gly Glu Ile Thr Arg Ser 
        355                 360                 365             


Met Lys Val Leu Gly Cys Tyr Pro Ser Glu Asn Val Val Pro Val Asp 
    370                 375                 380                 


Pro Thr 
385     


<210>  70
<211>  1122
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1122)

<400>  70
atg gtt gct gaa ttg acc gca tta cgc gat caa att gat gaa gtc gat         48
Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp           
1               5                   10                  15                

aaa gcg ctg ctg aat tta tta gcg aag cgt ctg gaa ctg gtt gct gaa         96
Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu           
            20                  25                  30                    

gtg ggc gag gtg aaa agc cgc ttt gga ctg cct att tat gtt ccg gag        144
Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu           
        35                  40                  45                        

cgc gag gca tct atg ttg gcc tcg cgt cgt gca gag gcg gaa gct ctg        192
Arg Glu Ala Ser Met Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu           
    50                  55                  60                            

ggt gta ccg cca gat ctg att gag gat gtt ttg cgt cgg gtg atg cgt        240
Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg           
65                  70                  75                  80            

gaa tct tac tcc agt gaa aac gac aaa gga ttt aaa aca ctt tgt ccg        288
Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro           
                85                  90                  95                

tca ctg cgt ccg gtg gtt atc gtc ggc ggt ggc ggt cag atg gga cgc        336
Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg           
            100                 105                 110                   

ctg ttc gag aag atg ctg acc ctc tcg ggt tat cag gtg cgg att ctg        384
Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu           
        115                 120                 125                       

gag caa cat gac tgg gat cga gcg gct gat att gtt gcc gat gcc gga        432
Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly           
    130                 135                 140                           

atg gtg att gtt agt gtg cca atc cac gtt act gag caa gtt att ggc        480
Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly           
145                 150                 155                 160           

aaa tta ccg cct tta ccg aaa gat tgt att ctg gtc gat ctg gca tca        528
Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser           
                165                 170                 175               

gtg aaa aat ggg cca tta cag gcc atg ctg gtg gcg cat gat ggt ccg        576
Val Lys Asn Gly Pro Leu Gln Ala Met Leu Val Ala His Asp Gly Pro           
            180                 185                 190                   

gtg ctg ggg cta cac ccg atg ttc ggt ccg gac agc ggt agc ctg gca        624
Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala           
        195                 200                 205                       

aag caa gtt gtg gtc tgg tgt gat gga cgt aaa ccg gaa gca tac caa        672
Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln           
    210                 215                 220                           

tgg ttt ctg gag caa att cag gtc tgg ggc gct cgg ctg cat cgt att        720
Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile           
225                 230                 235                 240           

agc gcc gtc gag cac gat cag aat atg gcg ttt att cag gca ctg cgc        768
Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg           
                245                 250                 255               

cac ttt gct act ttt gct tac ggg ctg cac ctg gca gaa gaa aat gtt        816
His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val           
            260                 265                 270                   

cag ctt gag caa ctt ctg gcg ctc tct tcg ccg att tac cgc ctt gag        864
Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu           
        275                 280                 285                       

ctg gcg atg gtc ggg cga ctg ttt gct cag gat ccg cag ctt tat gcc        912
Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala           
    290                 295                 300                           

gac atc att atg tcg tca gag cgt aat ctg gcg tta atc aaa cgt tac        960
Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr           
305                 310                 315                 320           

tat aag cgt ttc ggc gag gcg att gag ttg ctg gag cag ggc gat aag       1008
Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys           
                325                 330                 335               

cag gcg ttt att gac agt ttc cgc aag gtg gag cac tgg ttc ggc gat       1056
Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp           
            340                 345                 350                   

tac gca cag cgt ttt cag agt gaa agc cgc gtg tta ttg cgt cag gcg       1104
Tyr Ala Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala           
        355                 360                 365                       

aat gac aat cgc cag taa                                               1122
Asn Asp Asn Arg Gln                                                       
    370                                                                   


<210>  71
<211>  373
<212>  PRT
<213>  Escherichia coli

<400>  71

Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp 
1               5                   10                  15      


Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu 
            20                  25                  30          


Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu 
        35                  40                  45              


Arg Glu Ala Ser Met Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu 
    50                  55                  60                  


Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg 
65                  70                  75                  80  


Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro 
                85                  90                  95      


Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg 
            100                 105                 110         


Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu 
        115                 120                 125             


Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly 
    130                 135                 140                 


Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly 
145                 150                 155                 160 


Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser 
                165                 170                 175     


Val Lys Asn Gly Pro Leu Gln Ala Met Leu Val Ala His Asp Gly Pro 
            180                 185                 190         


Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala 
        195                 200                 205             


Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln 
    210                 215                 220                 


Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile 
225                 230                 235                 240 


Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg 
                245                 250                 255     


His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val 
            260                 265                 270         


Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu 
        275                 280                 285             


Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala 
    290                 295                 300                 


Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr 
305                 310                 315                 320 


Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys 
                325                 330                 335     


Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp 
            340                 345                 350         


Tyr Ala Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala 
        355                 360                 365             


Asn Asp Asn Arg Gln 
    370             


<210>  72
<211>  1716
<212>  DNA
<213>  Bacillus subtilis


<220>
<221>  CDS
<222>  (1)..(1716)

<400>  72
atg ttg aca aaa gca aca aaa gaa caa aaa tcc ctt gtg aaa aac aga         48
Met Leu Thr Lys Ala Thr Lys Glu Gln Lys Ser Leu Val Lys Asn Arg           
1               5                   10                  15                

ggg gcg gag ctt gtt gtt gat tgc tta gtg gag caa ggt gtc aca cat         96
Gly Ala Glu Leu Val Val Asp Cys Leu Val Glu Gln Gly Val Thr His           
            20                  25                  30                    

gta ttt ggc att cca ggt gca aaa att gat gcg gta ttt gac gct tta        144
Val Phe Gly Ile Pro Gly Ala Lys Ile Asp Ala Val Phe Asp Ala Leu           
        35                  40                  45                        

caa gat aaa gga cct gaa att atc gtt gcc cgg cac gaa caa aac gca        192
Gln Asp Lys Gly Pro Glu Ile Ile Val Ala Arg His Glu Gln Asn Ala           
    50                  55                  60                            

gca ttc atg gcc caa gca gtc ggc cgt tta act gga aaa ccg gga gtc        240
Ala Phe Met Ala Gln Ala Val Gly Arg Leu Thr Gly Lys Pro Gly Val           
65                  70                  75                  80            

gtg tta gtc aca tca gga ccg ggt gcc tct aac ttg gca aca ggc ctg        288
Val Leu Val Thr Ser Gly Pro Gly Ala Ser Asn Leu Ala Thr Gly Leu           
                85                  90                  95                

ctg aca gcg aac act gaa gga gac cct gtc gtt gcg ctt gct gga aac        336
Leu Thr Ala Asn Thr Glu Gly Asp Pro Val Val Ala Leu Ala Gly Asn           
            100                 105                 110                   

gtg atc cgt gca gat cgt tta aaa cgg aca cat caa tct ttg gat aat        384
Val Ile Arg Ala Asp Arg Leu Lys Arg Thr His Gln Ser Leu Asp Asn           
        115                 120                 125                       

gcg gcg cta ttc cag ccg att aca aaa tac agt gta gaa gtt caa gat        432
Ala Ala Leu Phe Gln Pro Ile Thr Lys Tyr Ser Val Glu Val Gln Asp           
    130                 135                 140                           

gta aaa aat ata ccg gaa gct gtt aca aat gca ttt agg ata gcg tca        480
Val Lys Asn Ile Pro Glu Ala Val Thr Asn Ala Phe Arg Ile Ala Ser           
145                 150                 155                 160           

gca ggg cag gct ggg gcc gct ttt gtg agc ttt ccg caa gat gtt gtg        528
Ala Gly Gln Ala Gly Ala Ala Phe Val Ser Phe Pro Gln Asp Val Val           
                165                 170                 175               

aat gaa gtc aca aat acg aaa aac gtg cgt gct gtt gca gcg cca aaa        576
Asn Glu Val Thr Asn Thr Lys Asn Val Arg Ala Val Ala Ala Pro Lys           
            180                 185                 190                   

ctc ggt cct gca gca gat gat gca atc agt gcg gcc ata gca aaa atc        624
Leu Gly Pro Ala Ala Asp Asp Ala Ile Ser Ala Ala Ile Ala Lys Ile           
        195                 200                 205                       

caa aca gca aaa ctt cct gtc gtt ttg gtc ggc atg aaa ggc gga aga        672
Gln Thr Ala Lys Leu Pro Val Val Leu Val Gly Met Lys Gly Gly Arg           
    210                 215                 220                           

ccg gaa gca att aaa gcg gtt cgc aag ctt ttg aaa aag gtt cag ctt        720
Pro Glu Ala Ile Lys Ala Val Arg Lys Leu Leu Lys Lys Val Gln Leu           
225                 230                 235                 240           

cca ttt gtt gaa aca tat caa gct gcc ggt acc ctt tct aga gat tta        768
Pro Phe Val Glu Thr Tyr Gln Ala Ala Gly Thr Leu Ser Arg Asp Leu           
                245                 250                 255               

gag gat caa tat ttt ggc cgt atc ggt ttg ttc cgc aac cag cct ggc        816
Glu Asp Gln Tyr Phe Gly Arg Ile Gly Leu Phe Arg Asn Gln Pro Gly           
            260                 265                 270                   

gat tta ctg cta gag cag gca gat gtt gtt ctg acg atc ggc tat gac        864
Asp Leu Leu Leu Glu Gln Ala Asp Val Val Leu Thr Ile Gly Tyr Asp           
        275                 280                 285                       

ccg att gaa tat gat ccg aaa ttc tgg aat atc aat gga gac cgg aca        912
Pro Ile Glu Tyr Asp Pro Lys Phe Trp Asn Ile Asn Gly Asp Arg Thr           
    290                 295                 300                           

att atc cat tta gac gag att atc gct gac att gat cat gct tac cag        960
Ile Ile His Leu Asp Glu Ile Ile Ala Asp Ile Asp His Ala Tyr Gln           
305                 310                 315                 320           

cct gat ctt gaa ttg atc ggt gac att ccg tcc acg atc aat cat atc       1008
Pro Asp Leu Glu Leu Ile Gly Asp Ile Pro Ser Thr Ile Asn His Ile           
                325                 330                 335               

gaa cac gat gct gtg aaa gtg gaa ttt gca gag cgt gag cag aaa atc       1056
Glu His Asp Ala Val Lys Val Glu Phe Ala Glu Arg Glu Gln Lys Ile           
            340                 345                 350                   

ctt tct gat tta aaa caa tat atg cat gaa ggt gag cag gtg cct gca       1104
Leu Ser Asp Leu Lys Gln Tyr Met His Glu Gly Glu Gln Val Pro Ala           
        355                 360                 365                       

gat tgg aaa tca gac aga gcg cac cct ctt gaa atc gtt aaa gag ttg       1152
Asp Trp Lys Ser Asp Arg Ala His Pro Leu Glu Ile Val Lys Glu Leu           
    370                 375                 380                           

cgt aat gca gtc gat gat cat gtt aca gta act tgc gat atc ggt tcg       1200
Arg Asn Ala Val Asp Asp His Val Thr Val Thr Cys Asp Ile Gly Ser           
385                 390                 395                 400           

cac gcc att tgg atg tca cgt tat ttc cgc agc tac gag ccg tta aca       1248
His Ala Ile Trp Met Ser Arg Tyr Phe Arg Ser Tyr Glu Pro Leu Thr           
                405                 410                 415               

tta atg atc agt aac ggt atg caa aca ctc ggc gtt gcg ctt cct tgg       1296
Leu Met Ile Ser Asn Gly Met Gln Thr Leu Gly Val Ala Leu Pro Trp           
            420                 425                 430                   

gca atc ggc gct tca ttg gtg aaa ccg gga gaa aaa gtg gtt tct gtc       1344
Ala Ile Gly Ala Ser Leu Val Lys Pro Gly Glu Lys Val Val Ser Val           
        435                 440                 445                       

tct ggt gac ggc ggt ttc tta ttc tca gca atg gaa tta gag aca gca       1392
Ser Gly Asp Gly Gly Phe Leu Phe Ser Ala Met Glu Leu Glu Thr Ala           
    450                 455                 460                           

gtt cga cta aaa gca cca att gta cac att gta tgg aac gac agc aca       1440
Val Arg Leu Lys Ala Pro Ile Val His Ile Val Trp Asn Asp Ser Thr           
465                 470                 475                 480           

tat gac atg gtt gca ttc cag caa ttg aaa aaa tat aac cgt aca tct       1488
Tyr Asp Met Val Ala Phe Gln Gln Leu Lys Lys Tyr Asn Arg Thr Ser           
                485                 490                 495               

gcg gtc gat ttc gga aat atc gat atc gtg aaa tat gcg gaa agc ttc       1536
Ala Val Asp Phe Gly Asn Ile Asp Ile Val Lys Tyr Ala Glu Ser Phe           
            500                 505                 510                   

gga gca act ggc ttg cgc gta gaa tca cca gac cag ctg gca gat gtt       1584
Gly Ala Thr Gly Leu Arg Val Glu Ser Pro Asp Gln Leu Ala Asp Val           
        515                 520                 525                       

ctg cgt caa ggc atg aac gct gaa ggt cct gtc atc atc gat gtc ccg       1632
Leu Arg Gln Gly Met Asn Ala Glu Gly Pro Val Ile Ile Asp Val Pro           
    530                 535                 540                           

gtt gac tac agt gat aac att aat tta gca agt gac aag ctt ccg aaa       1680
Val Asp Tyr Ser Asp Asn Ile Asn Leu Ala Ser Asp Lys Leu Pro Lys           
545                 550                 555                 560           

gaa ttc ggg gaa ctc atg aaa acg aaa gct ctc tag                       1716
Glu Phe Gly Glu Leu Met Lys Thr Lys Ala Leu                               
                565                 570                                   


<210>  73
<211>  571
<212>  PRT
<213>  Bacillus subtilis

<400>  73

Met Leu Thr Lys Ala Thr Lys Glu Gln Lys Ser Leu Val Lys Asn Arg 
1               5                   10                  15      


Gly Ala Glu Leu Val Val Asp Cys Leu Val Glu Gln Gly Val Thr His 
            20                  25                  30          


Val Phe Gly Ile Pro Gly Ala Lys Ile Asp Ala Val Phe Asp Ala Leu 
        35                  40                  45              


Gln Asp Lys Gly Pro Glu Ile Ile Val Ala Arg His Glu Gln Asn Ala 
    50                  55                  60                  


Ala Phe Met Ala Gln Ala Val Gly Arg Leu Thr Gly Lys Pro Gly Val 
65                  70                  75                  80  


Val Leu Val Thr Ser Gly Pro Gly Ala Ser Asn Leu Ala Thr Gly Leu 
                85                  90                  95      


Leu Thr Ala Asn Thr Glu Gly Asp Pro Val Val Ala Leu Ala Gly Asn 
            100                 105                 110         


Val Ile Arg Ala Asp Arg Leu Lys Arg Thr His Gln Ser Leu Asp Asn 
        115                 120                 125             


Ala Ala Leu Phe Gln Pro Ile Thr Lys Tyr Ser Val Glu Val Gln Asp 
    130                 135                 140                 


Val Lys Asn Ile Pro Glu Ala Val Thr Asn Ala Phe Arg Ile Ala Ser 
145                 150                 155                 160 


Ala Gly Gln Ala Gly Ala Ala Phe Val Ser Phe Pro Gln Asp Val Val 
                165                 170                 175     


Asn Glu Val Thr Asn Thr Lys Asn Val Arg Ala Val Ala Ala Pro Lys 
            180                 185                 190         


Leu Gly Pro Ala Ala Asp Asp Ala Ile Ser Ala Ala Ile Ala Lys Ile 
        195                 200                 205             


Gln Thr Ala Lys Leu Pro Val Val Leu Val Gly Met Lys Gly Gly Arg 
    210                 215                 220                 


Pro Glu Ala Ile Lys Ala Val Arg Lys Leu Leu Lys Lys Val Gln Leu 
225                 230                 235                 240 


Pro Phe Val Glu Thr Tyr Gln Ala Ala Gly Thr Leu Ser Arg Asp Leu 
                245                 250                 255     


Glu Asp Gln Tyr Phe Gly Arg Ile Gly Leu Phe Arg Asn Gln Pro Gly 
            260                 265                 270         


Asp Leu Leu Leu Glu Gln Ala Asp Val Val Leu Thr Ile Gly Tyr Asp 
        275                 280                 285             


Pro Ile Glu Tyr Asp Pro Lys Phe Trp Asn Ile Asn Gly Asp Arg Thr 
    290                 295                 300                 


Ile Ile His Leu Asp Glu Ile Ile Ala Asp Ile Asp His Ala Tyr Gln 
305                 310                 315                 320 


Pro Asp Leu Glu Leu Ile Gly Asp Ile Pro Ser Thr Ile Asn His Ile 
                325                 330                 335     


Glu His Asp Ala Val Lys Val Glu Phe Ala Glu Arg Glu Gln Lys Ile 
            340                 345                 350         


Leu Ser Asp Leu Lys Gln Tyr Met His Glu Gly Glu Gln Val Pro Ala 
        355                 360                 365             


Asp Trp Lys Ser Asp Arg Ala His Pro Leu Glu Ile Val Lys Glu Leu 
    370                 375                 380                 


Arg Asn Ala Val Asp Asp His Val Thr Val Thr Cys Asp Ile Gly Ser 
385                 390                 395                 400 


His Ala Ile Trp Met Ser Arg Tyr Phe Arg Ser Tyr Glu Pro Leu Thr 
                405                 410                 415     


Leu Met Ile Ser Asn Gly Met Gln Thr Leu Gly Val Ala Leu Pro Trp 
            420                 425                 430         


Ala Ile Gly Ala Ser Leu Val Lys Pro Gly Glu Lys Val Val Ser Val 
        435                 440                 445             


Ser Gly Asp Gly Gly Phe Leu Phe Ser Ala Met Glu Leu Glu Thr Ala 
    450                 455                 460                 


Val Arg Leu Lys Ala Pro Ile Val His Ile Val Trp Asn Asp Ser Thr 
465                 470                 475                 480 


Tyr Asp Met Val Ala Phe Gln Gln Leu Lys Lys Tyr Asn Arg Thr Ser 
                485                 490                 495     


Ala Val Asp Phe Gly Asn Ile Asp Ile Val Lys Tyr Ala Glu Ser Phe 
            500                 505                 510         


Gly Ala Thr Gly Leu Arg Val Glu Ser Pro Asp Gln Leu Ala Asp Val 
        515                 520                 525             


Leu Arg Gln Gly Met Asn Ala Glu Gly Pro Val Ile Ile Asp Val Pro 
    530                 535                 540                 


Val Asp Tyr Ser Asp Asn Ile Asn Leu Ala Ser Asp Lys Leu Pro Lys 
545                 550                 555                 560 


Glu Phe Gly Glu Leu Met Lys Thr Lys Ala Leu 
                565                 570     


