                         SEQUENCE LISTING

<110>  Terranol A/S
 
<120>  Microorganism

<130>  P035262WO

<150>  GB0822937.9
<151>  2008-12-16

<160>  28  

<170>  PatentIn version 3.5

<210>  1
<211>  1320
<212>  DNA
<213>  Lactococcus lactis subsp. lactis


<220>
<221>  CDS
<222>  (1)..(1320)

<400>  1
atg gct tac ttt aac gac atc gca cct atc aaa tac gaa ggt aca aaa         48
Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys           
1               5                   10                  15                

act aaa aat atg ttt gcc ttt cgc cat tat aat cca gaa gaa gta gtt         96
Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val           
            20                  25                  30                    

gct ggt aaa aca atg gaa gaa caa ctt cat ttt gcc ctt gca ttt tgg        144
Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp           
        35                  40                  45                        

cat aca att aca atg gat ggg tca gat ccc ttt ggg gga gca aca atg        192
His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met           
    50                  55                  60                            

gaa cgc cct tgg gat ttg gaa ggt ggt tct gaa ctt gac cgt gct cac        240
Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His           
65                  70                  75                  80            

cgt cga gta gat gct ttc ttt gaa att gct gaa aaa tta ggt gtt aaa        288
Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys           
                85                  90                  95                

tat tat tgt ttc cat gat att gat att gca cct act gga aat tct ttg        336
Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu           
            100                 105                 110                   

aaa gaa ttt tat gct aat ttg gac gaa att act gac cac ctt ctt gaa        384
Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu           
        115                 120                 125                       

aaa caa aaa gca aca ggg att aaa tta ctt tgg aat aca gca aac atg        432
Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met           
    130                 135                 140                           

ttt tca aat ccc cgc tat atg aat ggt gtt tca act tct aat cgt gct        480
Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala           
145                 150                 155                 160           

gaa gtc ttt gct tat ggt gct gca caa gtt aaa aaa ggt ctt gaa ctt        528
Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu           
                165                 170                 175               

tct aaa aaa ctc ggt ggt gaa aat tac gtc ttc tgg ggt ggt cgt gaa        576
Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu           
            180                 185                 190                   

ggt tat gaa tca ctt ttg aat aca gat atg ggt ctt gaa atg gat cat        624
Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His           
        195                 200                 205                       

atg gca aaa ttc ttc cat ttg gca att gat tat gca aaa tca atc aac        672
Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn           
    210                 215                 220                           

cac ttg ccc att ttc ttg att gaa cca aaa cca aaa gaa cca atg act        720
His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr           
225                 230                 235                 240           

cac caa tat gat ttt gac tca gca aca gct ctt gct ttc ttg caa aaa        768
His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys           
                245                 250                 255               

tat gat ttg gac aaa tat ttc aaa ctc aat ctt gaa aca aat cat gct        816
Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala           
            260                 265                 270                   

tgg ttg gct gga cac act ttt gaa cac gaa tta aat act gct cgt act        864
Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr           
        275                 280                 285                       

ttc aat gct ttg ggt tct att gat gcc aat caa gga aat tac ttg ctt        912
Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu           
    290                 295                 300                           

ggt tgg gat aca gat gaa ttc cca aca ctt gtt att gat atc aca ctt        960
Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu           
305                 310                 315                 320           

gcg atg cac caa att ctt ttg aac ggt gga ctt ggc aaa ggt gga att       1008
Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile           
                325                 330                 335               

aac ttt gat gcg aaa gta cgt cgt aca agt ttc aaa gca gaa gat tta       1056
Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu           
            340                 345                 350                   

att ctt gct cat att gca ggg atg gat act tat gcg cgt gct ttg aaa       1104
Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys           
        355                 360                 365                       

ggt gca gca gca atc att gaa gat aaa ttc ttg tct gat att gtt gac       1152
Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp           
    370                 375                 380                           

gaa cgt tat agt tca tac aaa aat aca gaa gtt ggt caa tcc att gaa       1200
Glu Arg Tyr Ser Ser Tyr Lys Asn Thr Glu Val Gly Gln Ser Ile Glu           
385                 390                 395                 400           

aat gga aca gca act ttt gaa agt ctt gcc gca ttt gca ctt gaa tat       1248
Asn Gly Thr Ala Thr Phe Glu Ser Leu Ala Ala Phe Ala Leu Glu Tyr           
                405                 410                 415               

ggt gat gat att gaa ctt gat tct aat cac ttg gaa tac atc aaa tca       1296
Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser           
            420                 425                 430                   

gta ttg aat gac tat ctt gtt taa                                       1320
Val Leu Asn Asp Tyr Leu Val                                               
        435                                                               


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
gctagccatg gcttacttta acgacatcgc acctatc                                37


<210>  3
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
ctcgagccta ggctaaacaa gatagtcatt caatactgat ttg                         43


<210>  4
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
gctagccatg gccactaccc catttgatgc tccagataag                             40


<210>  5
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ctcgagccta ggctagtgtt tcaattcact ttccatcttg gcc                         43


<210>  6
<211>  1383
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Thermoanaerobacter thermohydrosulfuricus xylose 
       isomerase gene


<220>
<221>  CDS
<222>  (49)..(1365)

<400>  6
gaattcctag aaataatttt gtttaacttt aagaaggagg agctagcc atg gaa tat        57
                                                     Met Glu Tyr          
                                                     1                    

ttc aaa aac gtg cca cag atc aag tat gaa ggt cct aaa agc aat aac        105
Phe Lys Asn Val Pro Gln Ile Lys Tyr Glu Gly Pro Lys Ser Asn Asn           
    5                   10                  15                            

cct tat gca ttt aag ttc tat aac cca gat gaa att ata gat gga aaa        153
Pro Tyr Ala Phe Lys Phe Tyr Asn Pro Asp Glu Ile Ile Asp Gly Lys           
20                  25                  30                  35            

cca tta aaa gaa cac tta aga ttt agc gta gcc tac tgg cat aca ttt        201
Pro Leu Lys Glu His Leu Arg Phe Ser Val Ala Tyr Trp His Thr Phe           
                40                  45                  50                

acc gct aac gga acg gat cca ttt ggt gca ccg act atg cag cgt cct        249
Thr Ala Asn Gly Thr Asp Pro Phe Gly Ala Pro Thr Met Gln Arg Pro           
            55                  60                  65                    

tgg gat cat ttt acc gac cct atg gac ata gca aaa gca cgt gtg gaa        297
Trp Asp His Phe Thr Asp Pro Met Asp Ile Ala Lys Ala Arg Val Glu           
        70                  75                  80                        

gcc gca ttc gag ctt ttt gaa aaa ttg gat gtt cca ttc ttc tgt ttt        345
Ala Ala Phe Glu Leu Phe Glu Lys Leu Asp Val Pro Phe Phe Cys Phe           
    85                  90                  95                            

cat gac aga gat ata gct ccg gaa ggt gaa aca ttg aga gaa acc aac        393
His Asp Arg Asp Ile Ala Pro Glu Gly Glu Thr Leu Arg Glu Thr Asn           
100                 105                 110                 115           

aaa aac tta gat act atc gtt gct atg att aaa gac tac tta aaa acg        441
Lys Asn Leu Asp Thr Ile Val Ala Met Ile Lys Asp Tyr Leu Lys Thr           
                120                 125                 130               

tca aag act aaa gtt ctt tgg ggc act gct aat ttg ttt tct aat cca        489
Ser Lys Thr Lys Val Leu Trp Gly Thr Ala Asn Leu Phe Ser Asn Pro           
            135                 140                 145                   

cgt ttc gtg cat ggc gct gcc aca tca tgt aat gca gac gta ttt gct        537
Arg Phe Val His Gly Ala Ala Thr Ser Cys Asn Ala Asp Val Phe Ala           
        150                 155                 160                       

tat gca gcc gct caa gtt aaa aag gcc tta gag att acc aaa gag tta        585
Tyr Ala Ala Ala Gln Val Lys Lys Ala Leu Glu Ile Thr Lys Glu Leu           
    165                 170                 175                           

gga ggc cag aat tat gtt ttc tgg ggt ggt cgt gag gga tat gag aca        633
Gly Gly Gln Asn Tyr Val Phe Trp Gly Gly Arg Glu Gly Tyr Glu Thr           
180                 185                 190                 195           

ctt tta aat act gat atg gag ttg gaa tta gat aat tta gca aga ttc        681
Leu Leu Asn Thr Asp Met Glu Leu Glu Leu Asp Asn Leu Ala Arg Phe           
                200                 205                 210               

tta cac atg gca gta gaa tat gct cag gaa att ggt ttt gaa gga cag        729
Leu His Met Ala Val Glu Tyr Ala Gln Glu Ile Gly Phe Glu Gly Gln           
            215                 220                 225                   

ttc ttg atc gag cct aaa cca aag gaa cca aca aag cat cag tat gat        777
Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys His Gln Tyr Asp           
        230                 235                 240                       

ttc gac gct gct tct gta cac gcc ttt ttg aag aag tat gat ttg gat        825
Phe Asp Ala Ala Ser Val His Ala Phe Leu Lys Lys Tyr Asp Leu Asp           
    245                 250                 255                           

aaa tac ttt aag ttg aac ata gag gct aat cac gca acg ttg gca ggt        873
Lys Tyr Phe Lys Leu Asn Ile Glu Ala Asn His Ala Thr Leu Ala Gly           
260                 265                 270                 275           

cac gat ttt caa cac gaa ttg aga tac gcc cgt att aat aac atg tta        921
His Asp Phe Gln His Glu Leu Arg Tyr Ala Arg Ile Asn Asn Met Leu           
                280                 285                 290               

ggt tcc ata gat gcc aac atg ggt gac atg ttg ctg ggt tgg gat act        969
Gly Ser Ile Asp Ala Asn Met Gly Asp Met Leu Leu Gly Trp Asp Thr           
            295                 300                 305                   

gat caa tac cca acg gat att aga atg aca act tta gca atg tac gag       1017
Asp Gln Tyr Pro Thr Asp Ile Arg Met Thr Thr Leu Ala Met Tyr Glu           
        310                 315                 320                       

gtc att aaa atg gga ggt ttt aac aaa gga ggt ttg aat ttc gat gct       1065
Val Ile Lys Met Gly Gly Phe Asn Lys Gly Gly Leu Asn Phe Asp Ala           
    325                 330                 335                           

aaa gtg cgt cgt gcc tct ttt gaa cct gaa gac ctt ttt ctt gga cat       1113
Lys Val Arg Arg Ala Ser Phe Glu Pro Glu Asp Leu Phe Leu Gly His           
340                 345                 350                 355           

att gcc gga atg gat gca ttt gca aaa ggt ttc aag gtc gct tat aag       1161
Ile Ala Gly Met Asp Ala Phe Ala Lys Gly Phe Lys Val Ala Tyr Lys           
                360                 365                 370               

ctt gtt aag gat ggt gta ttt gat aga ttc att gaa gag aga tac aaa       1209
Leu Val Lys Asp Gly Val Phe Asp Arg Phe Ile Glu Glu Arg Tyr Lys           
            375                 380                 385                   

tcc tat cgt gaa ggt ata ggt gct gaa atc gtt tca ggt aag gcc aat       1257
Ser Tyr Arg Glu Gly Ile Gly Ala Glu Ile Val Ser Gly Lys Ala Asn           
        390                 395                 400                       

ttt aag act tta gag gaa tat gca ttg aat aac cca aaa atc gaa aac       1305
Phe Lys Thr Leu Glu Glu Tyr Ala Leu Asn Asn Pro Lys Ile Glu Asn           
    405                 410                 415                           

aaa agc ggt aaa cag gaa ctg ctg gaa tct att ttg aat caa tat ttg       1353
Lys Ser Gly Lys Gln Glu Leu Leu Glu Ser Ile Leu Asn Gln Tyr Leu           
420                 425                 430                 435           

ttc tct gaa tag cctaggctcg aggaattc                                   1383
Phe Ser Glu                                                               
                                                                          


<210>  7
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Thermoanaerobacter thermosulphurigenes xylose 
       isomerase gene


<220>
<221>  CDS
<222>  (49)..(1368)

<400>  7
gaattcctag aaataatttt gtttaacttt aagaaggagg agctagcc atg gcc aag        57
                                                     Met Ala Lys          
                                                     1                    

tat ttt gag aat gtt tcc aag att aag tat gaa ggt cct aag tca aac        105
Tyr Phe Glu Asn Val Ser Lys Ile Lys Tyr Glu Gly Pro Lys Ser Asn           
    5                   10                  15                            

aac cct tac tcc ttc aaa ttc tat aat cca gaa gaa gtt ata gac ggt        153
Asn Pro Tyr Ser Phe Lys Phe Tyr Asn Pro Glu Glu Val Ile Asp Gly           
20                  25                  30                  35            

aaa acg atg gag gaa cac tta aga ttt tct att gct tac tgg cac aca        201
Lys Thr Met Glu Glu His Leu Arg Phe Ser Ile Ala Tyr Trp His Thr           
                40                  45                  50                

ttt act gcc gac ggc aca gac caa ttc gga aag gct act atg caa aga        249
Phe Thr Ala Asp Gly Thr Asp Gln Phe Gly Lys Ala Thr Met Gln Arg           
            55                  60                  65                    

ccg tgg aac cat tac act gat cca atg gac ata gcc aaa gcc aga gtg        297
Pro Trp Asn His Tyr Thr Asp Pro Met Asp Ile Ala Lys Ala Arg Val           
        70                  75                  80                        

gag gct gca ttc gag ttc ttc gat aag ata aac gcc cct tac ttc tgc        345
Glu Ala Ala Phe Glu Phe Phe Asp Lys Ile Asn Ala Pro Tyr Phe Cys           
    85                  90                  95                            

ttc cat gat cgt gac att gct ccg gaa ggc gat acc ttg aga gaa acc        393
Phe His Asp Arg Asp Ile Ala Pro Glu Gly Asp Thr Leu Arg Glu Thr           
100                 105                 110                 115           

aac aaa aat ctt gac acc att gtc gca atg ata aaa gat tat ttg aag        441
Asn Lys Asn Leu Asp Thr Ile Val Ala Met Ile Lys Asp Tyr Leu Lys           
                120                 125                 130               

acg tct aaa acc aaa gtt ttg tgg ggt acg gca aac tta ttt tct aat        489
Thr Ser Lys Thr Lys Val Leu Trp Gly Thr Ala Asn Leu Phe Ser Asn           
            135                 140                 145                   

ccg aga ttt gtt cat ggt gct tcc aca tcc tgc aac gca gat gtc ttt        537
Pro Arg Phe Val His Gly Ala Ser Thr Ser Cys Asn Ala Asp Val Phe           
        150                 155                 160                       

gct tat tcc gct gct caa gtc aaa aag gct ctt gag ata acc aaa gaa        585
Ala Tyr Ser Ala Ala Gln Val Lys Lys Ala Leu Glu Ile Thr Lys Glu           
    165                 170                 175                           

tta ggt ggt gaa aac tac gtt ttc tgg ggt ggc aga gaa ggt tat gag        633
Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu Gly Tyr Glu           
180                 185                 190                 195           

act ctt tta aat aca gat atg gaa ttt gaa ctt gac aat ttc gca aga        681
Thr Leu Leu Asn Thr Asp Met Glu Phe Glu Leu Asp Asn Phe Ala Arg           
                200                 205                 210               

ttc ttg cac atg gct gtc gat tat gcc aag gag ata ggt ttt gaa ggc        729
Phe Leu His Met Ala Val Asp Tyr Ala Lys Glu Ile Gly Phe Glu Gly           
            215                 220                 225                   

cag ttc ttg att gaa ccg aaa cca aag gaa cct acc aaa cat cag tat        777
Gln Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys His Gln Tyr           
        230                 235                 240                       

gac ttt gat gtc gca aat gtt ttg gct ttc ttg aga aaa tac gat tta        825
Asp Phe Asp Val Ala Asn Val Leu Ala Phe Leu Arg Lys Tyr Asp Leu           
    245                 250                 255                           

gat aag tat ttc aaa gta aat att gaa gct aat cat gca acg ttg gcc        873
Asp Lys Tyr Phe Lys Val Asn Ile Glu Ala Asn His Ala Thr Leu Ala           
260                 265                 270                 275           

ttc cat gat ttc cag cac gag ttg aga tac gct aga atc aat gga gtc        921
Phe His Asp Phe Gln His Glu Leu Arg Tyr Ala Arg Ile Asn Gly Val           
                280                 285                 290               

tta gga tct atc gat gct aat acc ggt gat atg ctg ctg gga tgg gat        969
Leu Gly Ser Ile Asp Ala Asn Thr Gly Asp Met Leu Leu Gly Trp Asp           
            295                 300                 305                   

act gat caa ttt ccg acc gat att cgt atg act acc ctg gca atg tat       1017
Thr Asp Gln Phe Pro Thr Asp Ile Arg Met Thr Thr Leu Ala Met Tyr           
        310                 315                 320                       

gaa gtt att aag atg ggt gga ttt gat aaa ggc ggt ctg aac ttc gat       1065
Glu Val Ile Lys Met Gly Gly Phe Asp Lys Gly Gly Leu Asn Phe Asp           
    325                 330                 335                           

gca aaa gta aga aga gct tct ttt gaa cct gaa gat ttg ttt tta gga       1113
Ala Lys Val Arg Arg Ala Ser Phe Glu Pro Glu Asp Leu Phe Leu Gly           
340                 345                 350                 355           

cac atc gct ggc atg gac gca ttt gct aaa ggt ttt aag gta gcc tat       1161
His Ile Ala Gly Met Asp Ala Phe Ala Lys Gly Phe Lys Val Ala Tyr           
                360                 365                 370               

aag ctt gta aaa gat aga gtg ttc gac aaa ttc atc gaa gag aga tat       1209
Lys Leu Val Lys Asp Arg Val Phe Asp Lys Phe Ile Glu Glu Arg Tyr           
            375                 380                 385                   

gct tct tat aag gac gga ata gga gcc gat ata gtt tcc ggt aag gcc       1257
Ala Ser Tyr Lys Asp Gly Ile Gly Ala Asp Ile Val Ser Gly Lys Ala           
        390                 395                 400                       

gat ttc aga tct ctt gag aaa tac gcc ttg gaa aga tca caa atc gtg       1305
Asp Phe Arg Ser Leu Glu Lys Tyr Ala Leu Glu Arg Ser Gln Ile Val           
    405                 410                 415                           

aac aaa tca ggc cgt caa gaa ttg tta gaa tca att ctt aat caa tac       1353
Asn Lys Ser Gly Arg Gln Glu Leu Leu Glu Ser Ile Leu Asn Gln Tyr           
420                 425                 430                 435           

ctg ttc gct gaa tag cctaggctcg aggaattc                               1386
Leu Phe Ala Glu                                                           
                                                                          


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
tagctagcat gtcgtacttc cccactgtcg ac                                     32


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
ataagctttc aggtgtagat aaagcggttg acc                                    33


<210>  10
<211>  1320
<212>  DNA
<213>  Lactococcus lactis subsp. lactis

<400>  10
atggcttact ttaacgacat cgcacctatc aaatacgaag gtacaaaaac taaaaatatg       60

tttgcctttc gtcattataa tccagaagaa gtagttgctg gtaaaacaat ggaagaacaa      120

cttcattttg cccttgcatt ttggcataca attacgatgg atgggtcaga tccctttggg      180

ggagcaacaa tggaacgtcc ttgggatttg gaaggtggtt ctgaacttga ccgtgctcac      240

cgtcgagtag atgctttctt tgaaattgct gaaaaattag gtgttaaata ttattgtttc      300

catgatattg atattgcacc tactggaaat tctttgaaag aattttatgc taatttggac      360

gaaattactg accaccttct tgaaaaacaa aaagcaacag gcattaaatt actttggaat      420

acagcaaaca tgttttcaaa tccccgctat atgaatggtg tttcaacttc taatcgtgct      480

gaagtctttg cttatggtgc tgcacaagtt aaaaaaggtc ttgaactttc taaaaaactc      540

ggtggtgaaa attatgtctt ctggggtggt cgtgaaggtt atgaatcact tttgaataca      600

gatatgggtc ttgaaatgga tcatatggca aaattcttcc atttggcaat tgattatgca      660

aaatcaatca accacttgcc tattttcttg attgaaccaa aaccaaaaga accaatgact      720

caccaatatg attttgactc agcaacagct cttgctttct tgcaaaaata tgacttggac      780

aaatacttca aactcaatct tgaaacaaat catgcttggt tggctgggca cacttttgaa      840

cacgaattaa atactgcacg tactttcaat gctttgggtt ctattgatgc caatcaagga      900

aattacttgc ttggttggga tacagatgaa ttcccaacac ttgttattga tatcacactt      960

gcgatgcacc aaattctttt gaacggtgga cttggcaaag gtggaattaa ctttgatgcg     1020

aaagtacgtc gtacaagttt caaagcagaa gatttaattc ttgctcatat tgcagggatg     1080

gatacttatg cgcgtgcttt gaaaggtgca gcagcaatca ttgaagataa attcttgtct     1140

gatattgttg acgaacgtta tagttcatac aaaaatacag aagttggtca atccattgaa     1200

aatggaacag caacttttga aagtcttgct gcatttgcac ttgaacatgg tgacgatatt     1260

gaacttgatt ctaatcactt ggaatacatc aaatcagtat tgaatgacta tcttgtttaa     1320


<210>  11
<211>  439
<212>  PRT
<213>  Lactococcus lactis subsp. lactis

<400>  11

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Lys Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Glu Ser Leu Ala Ala Phe Ala Leu Glu His 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  12
<211>  1320
<212>  DNA
<213>  Lactococcus lactis subsp. lactis

<400>  12
atggcttact ttaacgacat cgcacctatc aaatacgaag gtacaaaaac taaaaatatg       60

tttgcctttc gccattataa tccagaagaa gtagttgctg gtaaaacaat ggaagaacaa      120

cttcattttg cccttgcatt ttggcataca attacaatgg atgggtcaga cccctttggg      180

ggagcaacaa tggaacgtcc ttgggatttg gaaggtggtt ctgaacttga ccgtgctcac      240

cgtcgagtag atgctttctt tgaaattgct gaaaaattag gtgttaaata ttattgtttc      300

catgatattg atattgcacc tactggaaat tctttgaaag aattttatgc taatttggac      360

gaaattactg accaccttct tgaaaaacaa aaagcaacag ggattaaatt actttggaat      420

acagcaaaca tgttttcaaa tccccgctat atgaatggtg tttcaacttc taaccgtgct      480

gaagtctttg cttatggtgc tgcacaagtt aaaaaaggtc ttgaactttc taaaaaactc      540

ggtggtgaaa attacgtctt ctggggtggt cgtgaaggtt atgaatcact tttgaataca      600

gatatgggtc ttgaaatgga tcatatggca aaattcttcc atttggcaat tgattatgca      660

aaatcaatca accacttgcc cattttcttg atcgaaccaa aaccaaaaga accaatgact      720

caccaatatg attttgactc agcaacagct cttgctttct tgcaaaaata tgatttggac      780

aaatatttca aactcaatct tgaaacaaat catgcttggt tggctggaca cacttttgaa      840

cacgaattaa atactgctcg tactttcaat gctttgggtt ctattgatgc caatcaagga      900

aattacttgc ttggttggga tacagatgaa ttcccaacac ttgttattga tatcacactt      960

gcgatgcacc aaattctttt gaacggtgga cttggcaaag gtggaattaa ctttgatgcg     1020

aaagtacgtc gtacaagttt caaagcagaa gatttaattc ttgctcatat tgcagggatg     1080

gatacttatg cgcgtgcttt gaaaggtgca gcagcaatca ttgaagataa attcttgtct     1140

gatattgttg acgaacgtta tagttcatac aaaaatacag aagttggaca atccattgaa     1200

aatggaacag caacttttaa aagtcttgcc gcatttgcac ttgaacatgg tgacgatatt     1260

gaacttgatt ctaatcactt ggaatacatc aaatcagtat tgaatgacta tcttgtttaa     1320


<210>  13
<211>  439
<212>  PRT
<213>  Lactococcus lactis subsp. lactis

<400>  13

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Lys Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Lys Ser Leu Ala Ala Phe Ala Leu Glu His 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  14
<211>  439
<212>  PRT
<213>  Lactococcus lactis subsp. lactis

<400>  14

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Lys Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Glu Ser Leu Ala Ala Phe Ala Leu Glu Tyr 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  15
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Met Glu Tyr Phe Lys Asn Val Pro Gln Ile Lys Tyr Glu Gly Pro Lys 
1               5                   10                  15      


Ser Asn Asn Pro Tyr Ala Phe Lys Phe Tyr Asn Pro Asp Glu Ile Ile 
            20                  25                  30          


Asp Gly Lys Pro Leu Lys Glu His Leu Arg Phe Ser Val Ala Tyr Trp 
        35                  40                  45              


His Thr Phe Thr Ala Asn Gly Thr Asp Pro Phe Gly Ala Pro Thr Met 
    50                  55                  60                  


Gln Arg Pro Trp Asp His Phe Thr Asp Pro Met Asp Ile Ala Lys Ala 
65                  70                  75                  80  


Arg Val Glu Ala Ala Phe Glu Leu Phe Glu Lys Leu Asp Val Pro Phe 
                85                  90                  95      


Phe Cys Phe His Asp Arg Asp Ile Ala Pro Glu Gly Glu Thr Leu Arg 
            100                 105                 110         


Glu Thr Asn Lys Asn Leu Asp Thr Ile Val Ala Met Ile Lys Asp Tyr 
        115                 120                 125             


Leu Lys Thr Ser Lys Thr Lys Val Leu Trp Gly Thr Ala Asn Leu Phe 
    130                 135                 140                 


Ser Asn Pro Arg Phe Val His Gly Ala Ala Thr Ser Cys Asn Ala Asp 
145                 150                 155                 160 


Val Phe Ala Tyr Ala Ala Ala Gln Val Lys Lys Ala Leu Glu Ile Thr 
                165                 170                 175     


Lys Glu Leu Gly Gly Gln Asn Tyr Val Phe Trp Gly Gly Arg Glu Gly 
            180                 185                 190         


Tyr Glu Thr Leu Leu Asn Thr Asp Met Glu Leu Glu Leu Asp Asn Leu 
        195                 200                 205             


Ala Arg Phe Leu His Met Ala Val Glu Tyr Ala Gln Glu Ile Gly Phe 
    210                 215                 220                 


Glu Gly Gln Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys His 
225                 230                 235                 240 


Gln Tyr Asp Phe Asp Ala Ala Ser Val His Ala Phe Leu Lys Lys Tyr 
                245                 250                 255     


Asp Leu Asp Lys Tyr Phe Lys Leu Asn Ile Glu Ala Asn His Ala Thr 
            260                 265                 270         


Leu Ala Gly His Asp Phe Gln His Glu Leu Arg Tyr Ala Arg Ile Asn 
        275                 280                 285             


Asn Met Leu Gly Ser Ile Asp Ala Asn Met Gly Asp Met Leu Leu Gly 
    290                 295                 300                 


Trp Asp Thr Asp Gln Tyr Pro Thr Asp Ile Arg Met Thr Thr Leu Ala 
305                 310                 315                 320 


Met Tyr Glu Val Ile Lys Met Gly Gly Phe Asn Lys Gly Gly Leu Asn 
                325                 330                 335     


Phe Asp Ala Lys Val Arg Arg Ala Ser Phe Glu Pro Glu Asp Leu Phe 
            340                 345                 350         


Leu Gly His Ile Ala Gly Met Asp Ala Phe Ala Lys Gly Phe Lys Val 
        355                 360                 365             


Ala Tyr Lys Leu Val Lys Asp Gly Val Phe Asp Arg Phe Ile Glu Glu 
    370                 375                 380                 


Arg Tyr Lys Ser Tyr Arg Glu Gly Ile Gly Ala Glu Ile Val Ser Gly 
385                 390                 395                 400 


Lys Ala Asn Phe Lys Thr Leu Glu Glu Tyr Ala Leu Asn Asn Pro Lys 
                405                 410                 415     


Ile Glu Asn Lys Ser Gly Lys Gln Glu Leu Leu Glu Ser Ile Leu Asn 
            420                 425                 430         


Gln Tyr Leu Phe Ser Glu 
        435             


<210>  16
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Ala Lys Tyr Phe Glu Asn Val Ser Lys Ile Lys Tyr Glu Gly Pro 
1               5                   10                  15      


Lys Ser Asn Asn Pro Tyr Ser Phe Lys Phe Tyr Asn Pro Glu Glu Val 
            20                  25                  30          


Ile Asp Gly Lys Thr Met Glu Glu His Leu Arg Phe Ser Ile Ala Tyr 
        35                  40                  45              


Trp His Thr Phe Thr Ala Asp Gly Thr Asp Gln Phe Gly Lys Ala Thr 
    50                  55                  60                  


Met Gln Arg Pro Trp Asn His Tyr Thr Asp Pro Met Asp Ile Ala Lys 
65                  70                  75                  80  


Ala Arg Val Glu Ala Ala Phe Glu Phe Phe Asp Lys Ile Asn Ala Pro 
                85                  90                  95      


Tyr Phe Cys Phe His Asp Arg Asp Ile Ala Pro Glu Gly Asp Thr Leu 
            100                 105                 110         


Arg Glu Thr Asn Lys Asn Leu Asp Thr Ile Val Ala Met Ile Lys Asp 
        115                 120                 125             


Tyr Leu Lys Thr Ser Lys Thr Lys Val Leu Trp Gly Thr Ala Asn Leu 
    130                 135                 140                 


Phe Ser Asn Pro Arg Phe Val His Gly Ala Ser Thr Ser Cys Asn Ala 
145                 150                 155                 160 


Asp Val Phe Ala Tyr Ser Ala Ala Gln Val Lys Lys Ala Leu Glu Ile 
                165                 170                 175     


Thr Lys Glu Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Thr Leu Leu Asn Thr Asp Met Glu Phe Glu Leu Asp Asn 
        195                 200                 205             


Phe Ala Arg Phe Leu His Met Ala Val Asp Tyr Ala Lys Glu Ile Gly 
    210                 215                 220                 


Phe Glu Gly Gln Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Val Ala Asn Val Leu Ala Phe Leu Arg Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Val Asn Ile Glu Ala Asn His Ala 
            260                 265                 270         


Thr Leu Ala Phe His Asp Phe Gln His Glu Leu Arg Tyr Ala Arg Ile 
        275                 280                 285             


Asn Gly Val Leu Gly Ser Ile Asp Ala Asn Thr Gly Asp Met Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Gln Phe Pro Thr Asp Ile Arg Met Thr Thr Leu 
305                 310                 315                 320 


Ala Met Tyr Glu Val Ile Lys Met Gly Gly Phe Asp Lys Gly Gly Leu 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Ala Ser Phe Glu Pro Glu Asp Leu 
            340                 345                 350         


Phe Leu Gly His Ile Ala Gly Met Asp Ala Phe Ala Lys Gly Phe Lys 
        355                 360                 365             


Val Ala Tyr Lys Leu Val Lys Asp Arg Val Phe Asp Lys Phe Ile Glu 
    370                 375                 380                 


Glu Arg Tyr Ala Ser Tyr Lys Asp Gly Ile Gly Ala Asp Ile Val Ser 
385                 390                 395                 400 


Gly Lys Ala Asp Phe Arg Ser Leu Glu Lys Tyr Ala Leu Glu Arg Ser 
                405                 410                 415     


Gln Ile Val Asn Lys Ser Gly Arg Gln Glu Leu Leu Glu Ser Ile Leu 
            420                 425                 430         


Asn Gln Tyr Leu Phe Ala Glu 
        435                 


<210>  17
<211>  1320
<212>  DNA
<213>  Lactococcus lactis

<400>  17
atggcttact ttaacgacat cgcacctatc aaatacgaag gtacaaaaac taaaaatatg       60

tttgcctttc gccattataa tccagaagaa gtagttgctg gtaaaacaat ggaagaacaa      120

cttcattttg cccttgcatt ttggcataca attacaatgg atgggtcaga tccctttggg      180

ggagcaacaa tggaacgccc ttgggatttg gaaggtggtt ctgaacttga ccgtgctcac      240

cgtcgagtag atgctttctt tgaaattgct gaaaaattag gtgttaaata ttattgtttc      300

catgatattg atattgcacc tactggaaat tctttgaaag aattttatgc taatttggac      360

gaaattactg accaccttct tgaaaaacaa aaagcaacag ggattaaatt actttggaat      420

acagcaaaca tgttttcaaa tccccgctat atgaatggtg tttcaacttc taatcgtgct      480

gaagtctttg cttatggtgc tgcacaagtt aaaaaaggtc ttgaactttc taaaaaactc      540

ggtggtgaaa attacgtctt ctggggtggt cgtgaaggtt atgaatcact tttgaataca      600

gatatgggtc ttgaaatgga tcatatggca aaattcttcc atttggcaat tgattatgca      660

aaatcaatca accacttgcc cattttcttg attgaaccaa aaccaaaaga accaatgact      720

caccaatatg attttgactc agcaacagct cttgctttct tgcaaaaata tgatttggac      780

aaatatttca aactcaatct tgaaacaaat catgcttggt tggctggaca cacttttgaa      840

cacgaattaa atactgctcg tactttcaat gctttgggtt ctattgatgc caatcaagga      900

aattacttgc ttggttggga tacagatgaa ttcccaacac ttgttattga tatcacactt      960

gcgatgcacc aaattctttt gaacggtgga cttggcaaag gtggaattaa ctttgatgcg     1020

aaagtacgtc gtacaagttt caaagcagaa gatttaattc ttgctcatat tgcagggatg     1080

gatacttatg cgcgtgcttt gaaaggtgca gcagcaatca ttgaagataa attcttgtct     1140

gatattgttg acgaacgtta tagttcatac agaaatacag aagttggtca atccattgaa     1200

aatggaacag caacttttga aagtcttgcc gcatttgcac ttgaatatgg tgatgatatt     1260

gaacttgatt ctaatcactt ggaatacatc aaatcagtat tgaatgacta tcttgtttaa     1320


<210>  18
<211>  439
<212>  PRT
<213>  Lactococcus lactis

<400>  18

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Arg Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Glu Ser Leu Ala Ala Phe Ala Leu Glu Tyr 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  19
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of Lactococcus xylose isomerase

<400>  19

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Arg Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Lys Ser Leu Ala Ala Phe Ala Leu Glu His 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  20
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of Lactococcus xylose isomerase

<400>  20

Met Ala Tyr Phe Asn Asp Ile Ala Pro Ile Lys Tyr Glu Gly Thr Lys 
1               5                   10                  15      


Thr Lys Asn Met Phe Ala Phe Arg His Tyr Asn Pro Glu Glu Val Val 
            20                  25                  30          


Ala Gly Lys Thr Met Glu Glu Gln Leu His Phe Ala Leu Ala Phe Trp 
        35                  40                  45              


His Thr Ile Thr Met Asp Gly Ser Asp Pro Phe Gly Gly Ala Thr Met 
    50                  55                  60                  


Glu Arg Pro Trp Asp Leu Glu Gly Gly Ser Glu Leu Asp Arg Ala His 
65                  70                  75                  80  


Arg Arg Val Asp Ala Phe Phe Glu Ile Ala Glu Lys Leu Gly Val Lys 
                85                  90                  95      


Tyr Tyr Cys Phe His Asp Ile Asp Ile Ala Pro Thr Gly Asn Ser Leu 
            100                 105                 110         


Lys Glu Phe Tyr Ala Asn Leu Asp Glu Ile Thr Asp His Leu Leu Glu 
        115                 120                 125             


Lys Gln Lys Ala Thr Gly Ile Lys Leu Leu Trp Asn Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Tyr Met Asn Gly Val Ser Thr Ser Asn Arg Ala 
145                 150                 155                 160 


Glu Val Phe Ala Tyr Gly Ala Ala Gln Val Lys Lys Gly Leu Glu Leu 
                165                 170                 175     


Ser Lys Lys Leu Gly Gly Glu Asn Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Ser Leu Leu Asn Thr Asp Met Gly Leu Glu Met Asp His 
        195                 200                 205             


Met Ala Lys Phe Phe His Leu Ala Ile Asp Tyr Ala Lys Ser Ile Asn 
    210                 215                 220                 


His Leu Pro Ile Phe Leu Ile Glu Pro Lys Pro Lys Glu Pro Met Thr 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ser Ala Thr Ala Leu Ala Phe Leu Gln Lys 
                245                 250                 255     


Tyr Asp Leu Asp Lys Tyr Phe Lys Leu Asn Leu Glu Thr Asn His Ala 
            260                 265                 270         


Trp Leu Ala Gly His Thr Phe Glu His Glu Leu Asn Thr Ala Arg Thr 
        275                 280                 285             


Phe Asn Ala Leu Gly Ser Ile Asp Ala Asn Gln Gly Asn Tyr Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Thr Leu Val Ile Asp Ile Thr Leu 
305                 310                 315                 320 


Ala Met His Gln Ile Leu Leu Asn Gly Gly Leu Gly Lys Gly Gly Ile 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Thr Ser Phe Lys Ala Glu Asp Leu 
            340                 345                 350         


Ile Leu Ala His Ile Ala Gly Met Asp Thr Tyr Ala Arg Ala Leu Lys 
        355                 360                 365             


Gly Ala Ala Ala Ile Ile Glu Asp Lys Phe Leu Ser Asp Ile Val Asp 
    370                 375                 380                 


Glu Arg Tyr Ser Ser Tyr Arg Asn Thr Glu Val Gly Gln Ser Ile Glu 
385                 390                 395                 400 


Asn Gly Thr Ala Thr Phe Lys Ser Leu Ala Ala Phe Ala Leu Glu Tyr 
                405                 410                 415     


Gly Asp Asp Ile Glu Leu Asp Ser Asn His Leu Glu Tyr Ile Lys Ser 
            420                 425                 430         


Val Leu Asn Asp Tyr Leu Val 
        435                 


<210>  21
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
gttgacgaac gatatagttc atacaaaaat acagaagttg g                           41


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
ccaacttctg tatttttgta tgaactatat cgttcgtcaa c                           41


<210>  23
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
acagcaactt ttaaaagctt agccgcattt gcacttgaat atggtgatga tattg            55


<210>  24
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
caatatcatc accatattca agtgcaaatg cggctaagct tttaaaagtt gctgt            55


<210>  25
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
acagcaactt ttaaaagctt agccgcattt gcacttgaac atggtgatga tattg            55


<210>  26
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
caatatcatc accatgttca agtgcaaatg cggctaagct tttaaaagtt gctgt            55


<210>  27
<211>  1320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of Lactococcus xylose isomerase

<400>  27
atggcttact ttaacgacat cgcacctatc aaatacgaag gtacaaaaac taaaaatatg       60

tttgcctttc gccattataa tccagaagaa gtagttgctg gtaaaacaat ggaagaacaa      120

cttcattttg cccttgcatt ttggcataca attacaatgg atgggtcaga tccctttggg      180

ggagcaacaa tggaacgccc ttgggatttg gaaggtggtt ctgaacttga ccgtgctcac      240

cgtcgagtag atgctttctt tgaaattgct gaaaaattag gtgttaaata ttattgtttc      300

catgatattg atattgcacc tactggaaat tctttgaaag aattttatgc taatttggac      360

gaaattactg accaccttct tgaaaaacaa aaagcaacag ggattaaatt actttggaat      420

acagcaaaca tgttttcaaa tccccgctat atgaatggtg tttcaacttc taatcgtgct      480

gaagtctttg cttatggtgc tgcacaagtt aaaaaaggtc ttgaactttc taaaaaactc      540

ggtggtgaaa attacgtctt ctggggtggt cgtgaaggtt atgaatcact tttgaataca      600

gatatgggtc ttgaaatgga tcatatggca aaattcttcc atttggcaat tgattatgca      660

aaatcaatca accacttgcc cattttcttg attgaaccaa aaccaaaaga accaatgact      720

caccaatatg attttgactc agcaacagct cttgctttct tgcaaaaata tgatttggac      780

aaatatttca aactcaatct tgaaacaaat catgcttggt tggctggaca cacttttgaa      840

cacgaattaa atactgctcg tactttcaat gctttgggtt ctattgatgc caatcaagga      900

aattacttgc ttggttggga tacagatgaa ttcccaacac ttgttattga tatcacactt      960

gcgatgcacc aaattctttt gaacggtgga cttggcaaag gtggaattaa ctttgatgcg     1020

aaagtacgtc gtacaagttt caaagcagaa gatttaattc ttgctcatat tgcagggatg     1080

gatacttatg cgcgtgcttt gaaaggtgca gcagcaatca ttgaagataa attcttgtct     1140

gatattgttg acgaacgtta tagttcatac agaaatacag aagttggtca atccattgaa     1200

aatggaacag caacttttaa aagcttagcc gcatttgcac ttgaacatgg tgatgatatt     1260

gaacttgatt ctaatcactt ggaatacatc aaatcagtat tgaatgacta tcttgtttaa     1320


<210>  28
<211>  1320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of Lactococcus xylose isomerase

<400>  28
atggcttact ttaacgacat cgcacctatc aaatacgaag gtacaaaaac taaaaatatg       60

tttgcctttc gccattataa tccagaagaa gtagttgctg gtaaaacaat ggaagaacaa      120

cttcattttg cccttgcatt ttggcataca attacaatgg atgggtcaga tccctttggg      180

ggagcaacaa tggaacgccc ttgggatttg gaaggtggtt ctgaacttga ccgtgctcac      240

cgtcgagtag atgctttctt tgaaattgct gaaaaattag gtgttaaata ttattgtttc      300

catgatattg atattgcacc tactggaaat tctttgaaag aattttatgc taatttggac      360

gaaattactg accaccttct tgaaaaacaa aaagcaacag ggattaaatt actttggaat      420

acagcaaaca tgttttcaaa tccccgctat atgaatggtg tttcaacttc taatcgtgct      480

gaagtctttg cttatggtgc tgcacaagtt aaaaaaggtc ttgaactttc taaaaaactc      540

ggtggtgaaa attacgtctt ctggggtggt cgtgaaggtt atgaatcact tttgaataca      600

gatatgggtc ttgaaatgga tcatatggca aaattcttcc atttggcaat tgattatgca      660

aaatcaatca accacttgcc cattttcttg attgaaccaa aaccaaaaga accaatgact      720

caccaatatg attttgactc agcaacagct cttgctttct tgcaaaaata tgatttggac      780

aaatatttca aactcaatct tgaaacaaat catgcttggt tggctggaca cacttttgaa      840

cacgaattaa atactgctcg tactttcaat gctttgggtt ctattgatgc caatcaagga      900

aattacttgc ttggttggga tacagatgaa ttcccaacac ttgttattga tatcacactt      960

gcgatgcacc aaattctttt gaacggtgga cttggcaaag gtggaattaa ctttgatgcg     1020

aaagtacgtc gtacaagttt caaagcagaa gatttaattc ttgctcatat tgcagggatg     1080

gatacttatg cgcgtgcttt gaaaggtgca gcagcaatca ttgaagataa attcttgtct     1140

gatattgttg acgaacgtta tagttcatac agaaatacag aagttggtca atccattgaa     1200

aatggaacag caacttttaa aagcttagcc gcatttgcac ttgaatatgg tgatgatatt     1260

gaacttgatt ctaatcactt ggaatacatc aaatcagtat tgaatgacta tcttgtttaa     1320
