                         SEQUENCE LISTING

<110>  Centre National de la Recherche Scientifique (CNRS)
       Universite Claude Bernard Lyon 1
 
<120>  MONOCLONAL ANTIBODIES DIRECTED AGAINST LG4-5 DOMAIN OF ALPHA3 
       CHAIN OF HUMAN LAMININ-5

<130>  D27206

<150>  EP08305975.8
<151>  2008-12-18

<160>  40    

<170>  PatentIn version 3.3

<210>  1
<211>  1713
<212>  PRT
<213>  homo sapiens

<400>  1

Met Gly Trp Leu Trp Ile Phe Gly Ala Ala Leu Gly Gln Cys Leu Gly 
1               5                   10                  15      


Tyr Ser Ser Gln Gln Gln Arg Val Pro Phe Leu Gln Pro Pro Gly Gln 
            20                  25                  30          


Ser Gln Leu Gln Ala Ser Tyr Val Glu Phe Arg Pro Ser Gln Gly Cys 
        35                  40                  45              


Ser Pro Gly Tyr Tyr Arg Asp His Lys Gly Leu Tyr Thr Gly Arg Cys 
    50                  55                  60                  


Val Pro Cys Asn Cys Asn Gly His Ser Asn Gln Cys Gln Asp Gly Ser 
65                  70                  75                  80  


Gly Ile Cys Val Asn Cys Gln His Asn Thr Ala Gly Glu His Cys Glu 
                85                  90                  95      


Arg Cys Gln Glu Gly Tyr Tyr Gly Asn Ala Val His Gly Ser Cys Arg 
            100                 105                 110         


Ala Cys Pro Cys Pro His Thr Asn Ser Phe Ala Thr Gly Cys Val Val 
        115                 120                 125             


Asn Gly Gly Asp Val Arg Cys Ser Cys Lys Ala Gly Tyr Thr Gly Thr 
    130                 135                 140                 


Gln Cys Glu Arg Cys Ala Pro Gly Tyr Phe Gly Asn Pro Gln Lys Phe 
145                 150                 155                 160 


Gly Gly Ser Cys Gln Pro Cys Ser Cys Asn Ser Asn Gly Gln Leu Gly 
                165                 170                 175     


Ser Cys His Pro Leu Thr Gly Asp Cys Ile Asn Gln Glu Pro Lys Asp 
            180                 185                 190         


Ser Ser Pro Ala Glu Glu Cys Asp Asp Cys Asp Ser Cys Val Met Thr 
        195                 200                 205             


Leu Leu Asn Asp Leu Ala Thr Met Gly Glu Gln Leu Arg Leu Val Lys 
    210                 215                 220                 


Ser Gln Leu Gln Gly Leu Ser Ala Ser Ala Gly Leu Leu Glu Gln Met 
225                 230                 235                 240 


Arg His Met Glu Thr Gln Ala Lys Asp Leu Arg Asn Gln Leu Leu Asn 
                245                 250                 255     


Tyr Arg Ser Ala Ile Ser Asn His Gly Ser Lys Ile Glu Gly Leu Glu 
            260                 265                 270         


Arg Glu Leu Thr Asp Leu Asn Gln Glu Phe Glu Thr Leu Gln Glu Lys 
        275                 280                 285             


Ala Gln Val Asn Ser Arg Lys Ala Gln Thr Leu Asn Asn Asn Val Asn 
    290                 295                 300                 


Arg Ala Thr Gln Ser Ala Lys Glu Leu Asp Val Lys Ile Lys Asn Val 
305                 310                 315                 320 


Ile Arg Asn Val His Ile Leu Leu Lys Gln Ile Ser Gly Thr Asp Gly 
                325                 330                 335     


Glu Gly Asn Asn Val Pro Ser Gly Asp Phe Ser Arg Glu Trp Ala Glu 
            340                 345                 350         


Ala Gln Arg Met Met Arg Glu Leu Arg Asn Arg Asn Phe Gly Lys His 
        355                 360                 365             


Leu Arg Glu Ala Glu Ala Asp Lys Arg Glu Ser Gln Leu Leu Leu Asn 
    370                 375                 380                 


Arg Ile Arg Thr Trp Gln Lys Thr His Gln Gly Glu Asn Asn Gly Leu 
385                 390                 395                 400 


Ala Asn Ser Ile Arg Asp Ser Leu Asn Glu Tyr Glu Ala Lys Leu Ser 
                405                 410                 415     


Asp Leu Arg Ala Arg Leu Gln Glu Ala Ala Ala Gln Ala Lys Gln Ala 
            420                 425                 430         


Asn Gly Leu Asn Gln Glu Asn Glu Arg Ala Leu Gly Ala Ile Gln Arg 
        435                 440                 445             


Gln Val Lys Glu Ile Asn Ser Leu Gln Ser Asp Phe Thr Lys Tyr Leu 
    450                 455                 460                 


Thr Thr Ala Asp Ser Ser Leu Leu Gln Thr Asn Ile Ala Leu Gln Leu 
465                 470                 475                 480 


Met Glu Lys Ser Gln Lys Glu Tyr Glu Lys Leu Ala Ala Ser Leu Asn 
                485                 490                 495     


Glu Ala Arg Gln Glu Leu Ser Asp Lys Val Arg Glu Leu Ser Arg Ser 
            500                 505                 510         


Ala Gly Lys Thr Ser Leu Val Glu Glu Ala Glu Lys His Ala Arg Ser 
        515                 520                 525             


Leu Gln Glu Leu Ala Lys Gln Leu Glu Glu Ile Lys Arg Asn Ala Ser 
    530                 535                 540                 


Gly Asp Glu Leu Val Arg Cys Ala Val Asp Ala Ala Thr Ala Tyr Glu 
545                 550                 555                 560 


Asn Ile Leu Asn Ala Ile Lys Ala Ala Glu Asp Ala Ala Asn Arg Ala 
                565                 570                 575     


Ala Ser Ala Ser Glu Ser Ala Leu Gln Thr Val Ile Lys Glu Asp Leu 
            580                 585                 590         


Pro Arg Lys Ala Lys Thr Leu Ser Ser Asn Ser Asp Lys Leu Leu Asn 
        595                 600                 605             


Glu Ala Lys Met Thr Gln Lys Lys Leu Lys Gln Glu Val Ser Pro Ala 
    610                 615                 620                 


Leu Asn Asn Leu Gln Gln Thr Leu Asn Ile Val Thr Val Gln Lys Glu 
625                 630                 635                 640 


Val Ile Asp Thr Asn Leu Thr Thr Leu Arg Asp Gly Leu His Gly Ile 
                645                 650                 655     


Gln Arg Gly Asp Ile Asp Ala Met Ile Ser Ser Ala Lys Ser Met Val 
            660                 665                 670         


Arg Lys Ala Asn Asp Ile Thr Asp Glu Val Leu Asp Gly Leu Asn Pro 
        675                 680                 685             


Ile Gln Thr Asp Val Glu Arg Ile Lys Asp Thr Tyr Gly Arg Thr Gln 
    690                 695                 700                 


Asn Glu Asp Phe Lys Lys Ala Leu Thr Asp Ala Asp Asn Ser Val Asn 
705                 710                 715                 720 


Lys Leu Thr Asn Lys Leu Pro Asp Leu Trp Arg Lys Ile Glu Ser Ile 
                725                 730                 735     


Asn Gln Gln Leu Leu Pro Leu Gly Asn Ile Ser Asp Asn Met Asp Arg 
            740                 745                 750         


Ile Arg Glu Leu Ile Gln Gln Ala Arg Asp Ala Ala Ser Lys Val Ala 
        755                 760                 765             


Val Pro Met Arg Phe Asn Gly Lys Ser Gly Val Glu Val Arg Leu Pro 
    770                 775                 780                 


Asn Asp Leu Glu Asp Leu Lys Gly Tyr Thr Ser Leu Ser Leu Phe Leu 
785                 790                 795                 800 


Gln Arg Pro Asn Ser Arg Glu Asn Gly Gly Thr Glu Asn Met Phe Val 
                805                 810                 815     


Met Tyr Leu Gly Asn Lys Asp Ala Ser Arg Asp Tyr Ile Gly Met Ala 
            820                 825                 830         


Val Val Asp Gly Gln Leu Thr Cys Val Tyr Asn Leu Gly Asp Arg Glu 
        835                 840                 845             


Ala Glu Leu Gln Val Asp Gln Ile Leu Thr Lys Ser Glu Thr Lys Glu 
    850                 855                 860                 


Ala Val Met Asp Arg Val Lys Phe Gln Arg Ile Tyr Gln Phe Ala Arg 
865                 870                 875                 880 


Leu Asn Tyr Thr Lys Gly Ala Thr Ser Ser Lys Pro Glu Thr Pro Gly 
                885                 890                 895     


Val Tyr Asp Met Asp Gly Arg Asn Ser Asn Thr Leu Leu Asn Leu Asp 
            900                 905                 910         


Pro Glu Asn Val Val Phe Tyr Val Gly Gly Tyr Pro Pro Asp Phe Lys 
        915                 920                 925             


Leu Pro Ser Arg Leu Ser Phe Pro Pro Tyr Lys Gly Cys Ile Glu Leu 
    930                 935                 940                 


Asp Asp Leu Asn Glu Asn Val Leu Ser Leu Tyr Asn Phe Lys Lys Thr 
945                 950                 955                 960 


Phe Asn Leu Asn Thr Thr Glu Val Glu Pro Cys Arg Arg Arg Lys Glu 
                965                 970                 975     


Glu Ser Asp Lys Asn Tyr Phe Glu Gly Thr Gly Tyr Ala Arg Val Pro 
            980                 985                 990         


Thr Gln Pro His Ala Pro Ile Pro  Thr Phe Gly Gln Thr  Ile Gln Thr 
        995                 1000                 1005             


Thr Val  Asp Arg Gly Leu Leu  Phe Phe Ala Glu Asn  Gly Asp Arg 
    1010                 1015                 1020             


Phe Ile  Ser Leu Asn Ile Glu  Asp Gly Lys Leu Met  Val Arg Tyr 
    1025                 1030                 1035             


Lys Leu  Asn Ser Glu Leu Pro  Lys Glu Arg Gly Val  Gly Asp Ala 
    1040                 1045                 1050             


Ile Asn  Asn Gly Arg Asp His  Ser Ile Gln Ile Lys  Ile Gly Lys 
    1055                 1060                 1065             


Leu Gln  Lys Arg Met Trp Ile  Asn Val Asp Val Gln  Asn Thr Ile 
    1070                 1075                 1080             


Ile Asp  Gly Glu Val Phe Asp  Phe Ser Thr Tyr Tyr  Leu Gly Gly 
    1085                 1090                 1095             


Ile Pro  Ile Ala Ile Arg Glu  Arg Phe Asn Ile Ser  Thr Pro Ala 
    1100                 1105                 1110             


Phe Arg  Gly Cys Met Lys Asn  Leu Lys Lys Thr Ser  Gly Val Val 
    1115                 1120                 1125             


Arg Leu  Asn Asp Thr Val Gly  Val Thr Lys Lys Cys  Ser Glu Asp 
    1130                 1135                 1140             


Trp Lys  Leu Val Arg Ser Ala  Ser Phe Ser Arg Gly  Gly Gln Leu 
    1145                 1150                 1155             


Ser Phe  Thr Asp Leu Gly Leu  Pro Pro Thr Asp His  Leu Gln Ala 
    1160                 1165                 1170             


Ser Phe  Gly Phe Gln Thr Phe  Gln Pro Ser Gly Ile  Leu Leu Asp 
    1175                 1180                 1185             


His Gln  Thr Trp Thr Arg Asn  Leu Gln Val Thr Leu  Glu Asp Gly 
    1190                 1195                 1200             


Tyr Ile  Glu Leu Ser Thr Ser  Asp Ser Gly Gly Pro  Ile Phe Lys 
    1205                 1210                 1215             


Ser Pro  Gln Thr Tyr Met Asp  Gly Leu Leu His Tyr  Val Ser Val 
    1220                 1225                 1230             


Ile Ser  Asp Asn Ser Gly Leu  Arg Leu Leu Ile Asp  Asp Gln Leu 
    1235                 1240                 1245             


Leu Arg  Asn Ser Lys Arg Leu  Lys His Ile Ser Ser  Ser Arg Gln 
    1250                 1255                 1260             


Ser Leu  Arg Leu Gly Gly Ser  Asn Phe Glu Gly Cys  Ile Ser Asn 
    1265                 1270                 1275             


Val Phe  Val Gln Arg Leu Ser  Leu Ser Pro Glu Val  Leu Asp Leu 
    1280                 1285                 1290             


Thr Ser  Asn Ser Leu Lys Arg  Asp Val Ser Leu Gly  Gly Cys Ser 
    1295                 1300                 1305             


Leu Asn  Lys Pro Pro Phe Leu  Met Leu Leu Lys Gly  Ser Thr Arg 
    1310                 1315                 1320             


Phe Asn  Lys Thr Lys Thr Phe  Arg Ile Asn Gln Leu  Leu Gln Asp 
    1325                 1330                 1335             


Thr Pro  Val Ala Ser Pro Arg  Ser Val Lys Val Trp  Gln Asp Ala 
    1340                 1345                 1350             


Cys Ser  Pro Leu Pro Lys Thr  Gln Ala Asn His Gly  Ala Leu Gln 
    1355                 1360                 1365             


Phe Gly  Asp Ile Pro Thr Ser  His Leu Leu Phe Lys  Leu Pro Gln 
    1370                 1375                 1380             


Glu Leu  Leu Lys Pro Arg Ser  Gln Phe Ala Val Asp  Met Gln Thr 
    1385                 1390                 1395             


Thr Ser  Ser Arg Gly Leu Val  Phe His Thr Gly Thr  Lys Asn Ser 
    1400                 1405                 1410             


Phe Met  Ala Leu Tyr Leu Ser  Lys Gly Arg Leu Val  Phe Ala Leu 
    1415                 1420                 1425             


Gly Thr  Asp Gly Lys Lys Leu  Arg Ile Lys Ser Lys  Glu Lys Cys 
    1430                 1435                 1440             


Asn Asp  Gly Lys Trp His Thr  Val Val Phe Gly His  Asp Gly Glu 
    1445                 1450                 1455             


Lys Gly  Arg Leu Val Val Asp  Gly Leu Arg Ala Arg  Glu Gly Ser 
    1460                 1465                 1470             


Leu Pro  Gly Asn Ser Thr Ile  Ser Ile Arg Ala Pro  Val Tyr Leu 
    1475                 1480                 1485             


Gly Ser  Pro Pro Ser Gly Lys  Pro Lys Ser Leu Pro  Thr Asn Ser 
    1490                 1495                 1500             


Phe Val  Gly Cys Leu Lys Asn  Phe Gln Leu Asp Ser  Lys Pro Leu 
    1505                 1510                 1515             


Tyr Thr  Pro Ser Ser Ser Phe  Gly Val Ser Ser Cys  Leu Gly Gly 
    1520                 1525                 1530             


Pro Leu  Glu Lys Gly Ile Tyr  Phe Ser Glu Glu Gly  Gly His Val 
    1535                 1540                 1545             


Val Leu  Ala His Ser Val Leu  Leu Gly Pro Glu Phe  Lys Leu Val 
    1550                 1555                 1560             


Phe Ser  Ile Arg Pro Arg Ser  Leu Thr Gly Ile Leu  Ile His Ile 
    1565                 1570                 1575             


Gly Ser  Gln Pro Gly Lys His  Leu Cys Val Tyr Leu  Glu Ala Gly 
    1580                 1585                 1590             


Lys Val  Thr Ala Ser Met Asp  Ser Gly Ala Gly Gly  Thr Ser Thr 
    1595                 1600                 1605             


Ser Val  Thr Pro Lys Gln Ser  Leu Cys Asp Gly Gln  Trp His Ser 
    1610                 1615                 1620             


Val Ala  Val Thr Ile Lys Gln  His Ile Leu His Leu  Glu Leu Asp 
    1625                 1630                 1635             


Thr Asp  Ser Ser Tyr Thr Ala  Gly Gln Ile Pro Phe  Pro Pro Ala 
    1640                 1645                 1650             


Ser Thr  Gln Glu Pro Leu His  Leu Gly Gly Ala Pro  Ala Asn Leu 
    1655                 1660                 1665             


Thr Thr  Leu Arg Ile Pro Val  Trp Lys Ser Phe Phe  Gly Cys Leu 
    1670                 1675                 1680             


Arg Asn  Ile His Val Asn His  Ile Pro Val Pro Val  Thr Glu Ala 
    1685                 1690                 1695             


Leu Glu  Val Gln Gly Pro Val  Ser Leu Asn Gly Cys  Pro Asp Gln 
    1700                 1705                 1710             


<210>  2
<211>  183
<212>  PRT
<213>  homo sapiens

<400>  2

Met Gly Trp Leu Trp Ile Phe Gly Ala Ala Leu Gly Gln Cys Leu Gly 
1               5                   10                  15      


Tyr Ser Ser Gln Gln Gln Arg Val Pro Phe Leu Gln Pro Pro Gly Gln 
            20                  25                  30          


Ser Gln Leu Gln Ala Ser Tyr Val Glu Phe Arg Pro Ser Lys Val Ser 
        35                  40                  45              


Ser Tyr Gly Gly Tyr Leu Thr Tyr Gln Ala Lys Ser Phe Ala Leu Pro 
    50                  55                  60                  


Gly Asp Met Val Leu Leu Glu Lys Lys Pro Asp Val Gln Leu Thr Gly 
65                  70                  75                  80  


Gln His Met Ser Ile Ile Tyr Glu Glu Thr Asn Thr Pro Arg Pro Asp 
                85                  90                  95      


Arg Leu His His Gly Arg Val His Val Val Glu Gly Asn Phe Arg His 
            100                 105                 110         


Ala Ser Ser Arg Ala Pro Val Ser Arg Glu Glu Leu Met Thr Val Leu 
        115                 120                 125             


Ser Arg Leu Ala Asp Val Arg Ile Gln Gly Leu Tyr Phe Thr Glu Thr 
    130                 135                 140                 


Gln Arg Leu Thr Leu Ser Glu Val Gly Leu Glu Glu Ala Ser Asp Thr 
145                 150                 155                 160 


Gly Ser Gly Arg Ile Ala Leu Ala Val Glu Ile Cys Ala Cys Pro Pro 
                165                 170                 175     


Ala Tyr Ala Gly Asp Ser Cys 
            180             


<210>  3
<211>  353
<212>  PRT
<213>  artificial

<220>
<223>  LG4-5 domain of alpha 3 chain of human laminin-5

<400>  3

Gln Ala Asn His Gly Ala Leu Gln Phe Gly Asp Ile Pro Thr Ser His 
1               5                   10                  15      


Leu Leu Phe Lys Leu Pro Gln Glu Leu Leu Lys Pro Arg Ser Gln Phe 
            20                  25                  30          


Ala Val Asp Met Gln Thr Thr Ser Ser Arg Gly Leu Val Phe His Thr 
        35                  40                  45              


Gly Thr Lys Asn Ser Phe Met Ala Leu Tyr Leu Ser Lys Gly Arg Leu 
    50                  55                  60                  


Val Phe Ala Leu Gly Thr Asp Gly Lys Lys Leu Arg Ile Lys Ser Lys 
65                  70                  75                  80  


Glu Lys Cys Asn Asp Gly Lys Trp His Thr Val Val Phe Gly His Asp 
                85                  90                  95      


Gly Glu Lys Gly Arg Leu Val Val Asp Gly Leu Arg Ala Arg Glu Gly 
            100                 105                 110         


Ser Leu Pro Gly Asn Ser Thr Ile Ser Ile Arg Ala Pro Val Tyr Leu 
        115                 120                 125             


Gly Ser Pro Pro Ser Gly Lys Pro Lys Ser Leu Pro Thr Asn Ser Phe 
    130                 135                 140                 


Val Gly Cys Leu Lys Asn Phe Gln Leu Asp Ser Lys Pro Leu Tyr Thr 
145                 150                 155                 160 


Pro Ser Ser Ser Phe Gly Val Ser Ser Cys Leu Gly Gly Pro Leu Glu 
                165                 170                 175     


Lys Gly Ile Tyr Phe Ser Glu Glu Gly Gly His Val Val Leu Ala His 
            180                 185                 190         


Ser Val Leu Leu Gly Pro Glu Phe Lys Leu Val Phe Ser Ile Arg Pro 
        195                 200                 205             


Arg Ser Leu Thr Gly Ile Leu Ile His Ile Gly Ser Gln Pro Gly Lys 
    210                 215                 220                 


His Leu Cys Val Tyr Leu Glu Ala Gly Lys Val Thr Ala Ser Met Asp 
225                 230                 235                 240 


Ser Gly Ala Gly Gly Thr Ser Thr Ser Val Thr Pro Lys Gln Ser Leu 
                245                 250                 255     


Cys Asp Gly Gln Trp His Ser Val Ala Val Thr Ile Lys Gln His Ile 
            260                 265                 270         


Leu His Leu Glu Leu Asp Thr Asp Ser Ser Tyr Thr Ala Gly Gln Ile 
        275                 280                 285             


Pro Phe Pro Pro Ala Ser Thr Gln Glu Pro Leu His Leu Gly Gly Ala 
    290                 295                 300                 


Pro Ala Asn Leu Thr Thr Leu Arg Ile Pro Val Trp Lys Ser Phe Phe 
305                 310                 315                 320 


Gly Cys Leu Arg Asn Ile His Val Asn His Ile Pro Val Pro Val Thr 
                325                 330                 335     


Glu Ala Leu Glu Val Gln Gly Pro Val Ser Leu Asn Gly Cys Pro Asp 
            340                 345                 350         


Gln 
    


<210>  4
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 domain of Immunoglobulin light chain

<400>  4

Gln Ser Leu Leu Asp Ser Thr Gly Arg Thr Tyr 
1               5                   10      


<210>  5
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 domain of Immunoglobulin light chain

<400>  5

Trp Gln Gly Thr His Phe Pro His Thr 
1               5                   


<210>  6
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 domain of Immunoglobulin heavy chain

<400>  6

Gly Tyr Thr Phe Thr Glu Tyr Thr 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  CDR2 domain of Immunoglobulin heavy chain

<400>  7

Ile Asn Pro Lys Asn Gly Asp Thr 
1               5               


<210>  8
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 domain of Immunoglobulin heavy chain

<400>  8

Ala Ser Pro Asp Leu Pro Pro Met Asp Tyr 
1               5                   10  


<210>  9
<211>  112
<212>  PRT
<213>  artificial

<220>
<223>  LCVR immunoglobulin

<400>  9

Asp Ile Gln Leu Thr Gln Ser Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Thr Gly Arg Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Leu Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  10
<211>  116
<212>  PRT
<213>  artificial

<220>
<223>  HCVR Immunoglobulin

<400>  10

Val Lys Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Glu Tyr Thr 
            20                  25                  30          


Ile His Trp Val Lys Gln Ser His Gly Lys Thr Leu Glu Trp Ile Gly 
        35                  40                  45              


Gly Ile Asn Pro Lys Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
    50                  55                  60                  


Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Asn Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Phe Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ser Pro Asp Leu Pro Pro Met Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  11
<211>  111
<212>  PRT
<213>  artificial

<220>
<223>  Humanized LCVR Immunoglobulin


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  X = Q, V or E

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  X = T or S

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  X = S or P

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  X = I or L

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  X = K or R

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  X = L or F

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  X = L, Q or H

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  X = K or R

<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  X = K, N, D or H

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  X = L, R or W

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  X = T or S

<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  X = L or V

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  X = L or V

<400>  11

Asp Ile Xaa Leu Thr Gln Ser Pro Leu Xaa Leu Xaa Val Thr Xaa Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Xaa Ser Ser Gln Ser Leu Leu Ser Thr 
            20                  25                  30          


Gly Arg Thr Tyr Leu Asn Trp Xaa Xaa Gln Arg Pro Gly Gln Ser Pro 
        35                  40                  45              


Xaa Arg Leu Ile Tyr Leu Val Ser Xaa Xaa Asp Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Xaa Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser 
65                  70                  75                  80  


Arg Val Glu Ala Glu Asp Xaa Gly Xaa Tyr Tyr Cys Trp Gln Gly Thr 
                85                  90                  95      


His Phe Pro His Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  12
<211>  116
<212>  PRT
<213>  artificial

<220>
<223>  Humanized HCVR Immunoglobulin


<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  X = T, A or V

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  X = I, M or V

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  X = G, D, Y, M, W, E or I

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  X = F or L

<400>  12

Val Lys Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Xaa Ser Gly Tyr Thr Phe Thr Glu Tyr Thr 
            20                  25                  30          


Xaa His Trp Val Lys Gln Ser His Gly Lys Thr Leu Glu Trp Ile Gly 
        35                  40                  45              


Xaa Ile Asn Pro Lys Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
    50                  55                  60                  


Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Asn Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Xaa Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ser Pro Asp Leu Pro Pro Met Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  13
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  VH-FOR PCR primer

<400>  13
tgaggagacg gtgaccgtgg tcccttggcc ccag                                 34


<210>  14
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  VH-BACK PCR primer

<400>  14
aggtsmarct gcagsagtcw gg                                              22


<210>  15
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  VL-FOR PCR primer

<400>  15
gttagatctc cagcttggtc cc                                              22


<210>  16
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  VL-BACK PCR primer

<400>  16
gacattcagc tgacccagtc tcca                                            24


<210>  17
<211>  396
<212>  DNA
<213>  mus musculus


<220>
<221>  misc_feature
<223>  3A11-VH domain

<400>  17
gaggtccaac tgcagcagtc agggggaggc ttagtgcagc ctggagggtc cctgaaactc     60

tcctgtgcaa cctctggatt cactttcagt gactattaca tgttttgggt tcgccagact    120

ccagagaaga ggctggagtg ggtcgcacac attaccaata taggtggtaa cacctattat    180

ccagacactg taaagggccg attcaccatc tccagagaca atgacaagaa caccctgtac    240

ctgcaaatga gccgtctgaa gtctgaagac acagccatgt attactgtgc aagacccccc    300

tcctatggta actacgggta cttcaatgtc tggggccaag ggaccacggt caccgtctcc    360

tcactggggc caagggacca cggtcaccgc tcctca                              396


<210>  18
<211>  132
<212>  PRT
<213>  mus musculus


<220>
<221>  misc_feature
<223>  3A11-VH domain

<400>  18

Glu Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Thr Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Tyr Met Phe Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala His Ile Thr Asn Ile Gly Gly Asn Thr Tyr Tyr Pro Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Arg Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Pro Ser Tyr Gly Asn Tyr Gly Tyr Phe Asn Val Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Val Thr Val Ser Ser Leu Gly Pro Arg Asp His Gly 
        115                 120                 125             


His Arg Ser Ser 
    130         


<210>  19
<211>  372
<212>  DNA
<213>  mus musculus


<220>
<221>  misc_feature
<223>  3A11-VL domain

<400>  19
gacattgtgg tcattcaatc tccagcttct ttggctgtgt ctctagggca gagagccacc     60

atctcctgca gagccagtga aagtgttgaa tattatggca caagtttaat gcagtggtac    120

caacagaaac caggacagcc acccaaactc ctcatctata ctgcgtccaa cgtagaatct    180

ggggtccctg ccaggtttag tggcagtggg tctgggacag acttcagcct caacatccat    240

cctgtggagg aggatgatat tgcaatgtat ttctgtcagc aaagtaggaa ggttccgtac    300

acgttcggag gggggaccaa gctggaaata aaacgggctg atgctgcacc aactgtatcc    360

atcttcccac ca                                                        372


<210>  20
<211>  124
<212>  PRT
<213>  mus musculus


<220>
<221>  misc_feature
<223>  3A11-VL domain

<400>  20

Asp Ile Val Val Ile Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr 
            20                  25                  30          


Gly Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Ala Ser Asn Val Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg 
                85                  90                  95      


Lys Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
        115                 120                 


<210>  21
<211>  351
<212>  DNA
<213>  mus musculus


<220>
<221>  misc_feature
<223>  15G5-VH domain

<400>  21
tcagggactg tgctggcaag gcctggggct tccgtgagga tgtcctgcaa ggcttctggc     60

tacagcttag ccacctactg gatgcactgg gtaaaacaga ggcctggaca gggtctagaa    120

tggattggtt ctatttatcc tggaaatggt gaaactacct acaaccagaa gttcaaggac    180

aaggccagac tgactgcagt cacatccgcc agcactgcct acatggagtt cagcagcctg    240

acaattgagg actctgcggt ctattactgt acaagagaga gggccgacgt atattactat    300

ggtatggact attggggcca agggaccacg gtcaccgtct cctcaaaggg c             351


<210>  22
<211>  117
<212>  PRT
<213>  mus musculus


<220>
<221>  misc_feature
<223>  15G5-VH domain

<400>  22

Ser Gly Thr Val Leu Ala Arg Pro Gly Ala Ser Val Arg Met Ser Cys 
1               5                   10                  15      


Lys Ala Ser Gly Tyr Ser Leu Ala Thr Tyr Trp Met His Trp Val Lys 
            20                  25                  30          


Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Ser Ile Tyr Pro Gly 
        35                  40                  45              


Asn Gly Glu Thr Thr Tyr Asn Gln Lys Phe Lys Asp Lys Ala Arg Leu 
    50                  55                  60                  


Thr Ala Val Thr Ser Ala Ser Thr Ala Tyr Met Glu Phe Ser Ser Leu 
65                  70                  75                  80  


Thr Ile Glu Asp Ser Ala Val Tyr Tyr Cys Thr Arg Glu Arg Ala Asp 
                85                  90                  95      


Val Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Lys Gly 
        115         


<210>  23
<211>  357
<212>  DNA
<213>  mus musculus


<220>
<221>  misc_feature
<223>  15G5-VL domain

<400>  23
caaattgttc tcacccagtc tccagcaatc atgtctgcat ctccagggga gaaggtctcc     60

ataacctgca gtgccagctc aagtatagct cacatgtact ggttccagca gaagccagac    120

acttctccca aactctggat ttacagcaca tccaacctgg cttctggagt cccttctcgc    180

ttcagtggca gtggatctgg gacctcttac tctctcacaa tcagccgaat ggaggctgaa    240

gatgctgcca cttattactg ccaccaaagg agtagttacc cattcacgtt cggctcgggg    300

acaaagttgg aagtaaaacg ggctgatgct gcaccaactg tatccatctt cccacca       357


<210>  24
<211>  119
<212>  PRT
<213>  mus musculus


<220>
<221>  misc_feature
<223>  15G5-VL domain

<400>  24

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Ser Ile Thr Cys Ser Ala Ser Ser Ser Ile Ala His Met 
            20                  25                  30          


Tyr Trp Phe Gln Gln Lys Pro Asp Thr Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys His Gln Arg Ser Ser Tyr Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Val Lys Arg Ala Asp Ala Ala Pro 
            100                 105                 110         


Thr Val Ser Ile Phe Pro Pro 
        115                 


<210>  25
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  1H12 mAb epitope from LG4/5

<400>  25

Asp Ser Lys Pro Leu Tyr Thr Pro Ser Ser Ser Phe Gly Val 
1               5                   10                  


<210>  26
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  1H12 mAb epitope from Family 1

<400>  26

Tyr Gln Glu Glu Thr Pro Ala Ser Ser Phe Ser Arg 
1               5                   10          


<210>  27
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  1H12 mAb epitope from Family 2

<400>  27

Leu Pro Val Pro Ala Tyr Asn Met Ser Ser Lys Phe 
1               5                   10          


<210>  28
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  1H12 mAb epitope from Family 3

<400>  28

Ala Pro Gly Lys Ala Thr Trp Pro Ser Ser Lys Phe 
1               5                   10          


<210>  29
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR1 from 3A11-VH domain

<400>  29

Gly Phe Thr Phe Ser Asp Tyr Tyr 
1               5               


<210>  30
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR2 from 3A11-VH domain

<400>  30

Ile Thr Asn Ile Gly Gly Asn Thr 
1               5               


<210>  31
<211>  14
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR3 from 3A11-VH domain

<400>  31

Ala Arg Pro Pro Ser Tyr Gly Asn Tyr Gly Tyr Phe Asn Val 
1               5                   10                  


<210>  32
<211>  10
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR1 from 3A11-VL domain

<400>  32

Glu Ser Val Glu Tyr Tyr Gly Thr Ser Leu 
1               5                   10  


<210>  33
<211>  3
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR2 from 3A11-VL domain

<400>  33

Thr Ala Ser 
1           


<210>  34
<211>  9
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR3 from 3A11-VL domain

<400>  34

Gln Gln Ser Arg Lys Val Pro Tyr Thr 
1               5                   


<210>  35
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR1 from 15G5-VH domain

<400>  35

Gly Tyr Ser Leu Ala Thr Tyr Trp 
1               5               


<210>  36
<211>  8
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR2 from 15G5-VH domain

<400>  36

Ile Tyr Pro Gly Asn Gly Glu Thr 
1               5               


<210>  37
<211>  14
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR3 from 15G5-VH domain

<400>  37

Thr Arg Glu Arg Ala Asp Val Tyr Tyr Tyr Gly Met Asp Tyr 
1               5                   10                  


<210>  38
<211>  5
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR1 from 15G5-VL domain

<400>  38

Ser Ser Ile Ala His 
1               5   


<210>  39
<211>  3
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR2 from 15G5-VL domain

<400>  39

Ser Thr Ser 
1           


<210>  40
<211>  9
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  CDR3 from 15G5-VL domain

<400>  40

His Gln Arg Ser Ser Tyr Pro Phe Thr 
1               5                   


